Uba5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba5
Entrez Gene ID	300968
Full Name	ubiquitin-like modifier activating enzyme 5
Synonyms	Ube1dc1
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 5 Names ubiquitin-like modifier-activating enzyme 5 UFM1-activating enzyme ubiquitin-activating enzyme 5 ubiquitin-activating enzyme E1 domain-containing protein 1 ubiquitin-activating enzyme E1-domain containing 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595592.1	XP_017451081.1	ubiquitin-like modifier-activating enzyme 5 isoform X1
NM_001009669.1	NP_001009669.1	ubiquitin-like modifier-activating enzyme 5

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0506500	NP_001046919.1
Homo sapiens (human)	UBA5	NP_079094.1
Mus musculus (house mouse)	Uba5	NP_079968.2
Rattus norvegicus (Norway rat)	Uba5	NP_001009669.1
Danio rerio (zebrafish)	uba5	XP_691141.2
Drosophila melanogaster (fruit fly)	CG1749	NP_572722.2
Caenorhabditis elegans (roundworm)	T03F1.1	NP_491248.1
Pan troglodytes (chimpanzee)	UBA5	XP_516757.2
Xenopus tropicalis (tropical clawed frog)	uba5	NP_001004790.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009012	XP_319759.4
Bos taurus (cattle)	UBA5	NP_001094537.1
Gallus gallus (chicken)	UBA5	NP_001001765.1
Arabidopsis thaliana (thale cress)	AT1G05350	NP_172027.2
Canis lupus familiaris (dog)	UBA5	XP_003639874.1

Gene Ontology
Bibliography

Related articles in PubMed

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Colin E, Daniel J, Ziegler A, Wakim J, Scrivo A, Haack TB, Khiati S, Denommé AS, Amati-Bonneau P, Charif M, Procaccio V, Reynier P, Aleck KA, Botto LD, Herper CL, Kaiser CS, Nabbout R, N'Guyen S, Mora-Lorca JA, Assmann B, Christ S, Meitinger T, Strom TM, Prokisch H, , Miranda-Vizuete A, Hoffmann GF, Lenaers G, Bomont P, Liebau E, Bonneau D
American journal of human genetics99(3)695-703(2016 09)

UBA5 Mutations Cause a New Form of Autosomal Recessive Cerebellar Ataxia.
Duan R, Shi Y, Yu L, Zhang G, Li J, Lin Y, Guo J, Wang J, Shen L, Jiang H, Wang G, Tang B
PloS one11(2)e0149039(2016)

Modification of ASC1 by UFM1 is crucial for ERα transactivation and breast cancer development.
Yoo HM, Kang SH, Kim JY, Lee JE, Seong MW, Lee SW, Ka SH, Sou YS, Komatsu M, Tanaka K, Lee ST, Noh DY, Baek SH, Jeon YJ, Chung CH
Molecular cell56(2)261-274(2014 Oct)

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
Zhang Y, Zhang M, Wu J, Lei G, Li H
PloS one7(11)e48587(2012)

A novel type of E3 ligase for the Ufm1 conjugation system.
Tatsumi K, Sou YS, Tada N, Nakamura E, Iemura S, Natsume T, Kang SH, Chung CH, Kasahara M, Kominami E, Yamamoto M, Tanaka K, Komatsu M
The Journal of biological chemistry285(8)5417-27(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Uba5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00782	NM_001009669.1 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa62394	XM_017595592.1 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba5 ( NM_001009669.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009669.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), mRNA.

Price & Availability↑

CloneID	ORa00782
Clone ID Related Accession (Same CDS sequence)	NM_001009669.1
Accession Version	NM_001009669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088757.1. On Jan 13, 2005 this sequence version replaced XM_217245.2. ##Evidence-Data-START## Transcript exon combination :: BC088757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGAGT CGGTGGAGCG GCTGCTGCAG CGGGTTGAGG AGCTGGAGCA GGAACTCGCC 
CGGGAGAGAA GCCGGCGGAT TGCGGGGGAC GGCCACTGCG GCCGGACCCG CATCCAGAAG 
ATGAGCGACG AGGTGGTGGA TTCCAATCCC TACAGCCGTC TGATGGCATT GAAACGGATG 
GGAGTTGTCA GTGACTATGA GAAAATCCGT ACCTATGCTG TAGCAATAGT AGGTGTTGGT 
GGAGTTGGTA GTGTGACTGC TGAAATGCTG ACAAGATGTG GCATTGGTAA GTTGCTACTC 
TTTGACTATG ACAAGGTGGA GCTGGCCAAC ATGAATCGAC TTTTCTTCCA GCCGTATCAA 
GCAGGGATGA GTAAGGTCCA AGCAGCAGAG CACACCCTGA GGAGCATTAA CCCTGATGTT 
CTGTTTGAAG TTCACAACTA TAATATCACC ACCGTGGAAC ACTTCGAACA TTTCATGAAT 
AGAATAAGTA ATGGGGGATT AGAAGAAGGA CAACCTGTTG ACCTAGTTCT TAGCTGTGTG 
GACAACTTTG AAGCTCGAAT GGCAATAAAT ACAGCTTGTA ATGAACTTGG TCAGACATGG 
ATGGAATCTG GGGTCAGTGA AAACGCAGTT TCCGGGCACA TCCAGCTTAT GGTCCCTGGA 
GAGTCGGCTT GCTTTGCGTG CGCACCACCA CTTGTAGTTG CTTCAAACAT TGATGAAAAA 
ACCCTGAAAC GAGAAGGCGT CTGCGCGGCC AGCCTCCCCA CCACCATGGG AGTGGTTGCA 
GGGATCTTGG TACAAAATGT ATTAAAGTTT CTGTTAAAAT TTGGGACTGT CAGTTTTTAC 
CTCGGATACA ATGCCATGCA GGACTTCTTC CCTACCATGT TCATGAAGCC AAACCCTCAG 
TGTGATGACA AAAACTGTAG GAAGCAACAA GAAGAATACA AGAAGAGGGC ACCAGCGCAG 
CCCACGCAGG AGACTGCGCC CCAAGAGGAG GAAGAGGTAG TTCATGAGGA CAACGAGTGG 
GGTATTGAGC TGGTGTCTGA GGTTTCAGAA GAGGAACTGA AGAACTCGTC AGGTCCTGTT 
CCCACCTTAC CTGAAGGAAT TACAGTGGCA TACACAGTCC CAAAGAAGAG AGAGGATTCT 
GTTTCTGAAG TAACGGTGGA AGATTCTGGT GAAAGCTTGG AAGATCTCAT GGCCAGGATG 
AAGAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009669.1
CDS	130..1341
Translation	MAESVERLLQRVEELEQELARERSRRIAGDGHCGRTRIQKMSDEVVDSNPYSRLMALKRMGVVSDYEKIRTYAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPYQAGMSKVQAAEHTLRSINPDVLFEVHNYNITTVEHFEHFMNRISNGGLEEGQPVDLVLSCVDNFEARMAINTACNELGQTWMESGVSENAVSGHIQLMVPGESACFACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYNAMQDFFPTMFMKPNPQCDDKNCRKQQEEYKKRAPAQPTQETAPQEEEEVVHEDNEWGIELVSEVSEEELKNSSGPVPTLPEGITVAYTVPKKREDSVSEVTVEDSGESLEDLMARMKKM

Target ORF information:

RefSeq Version	NM_001009669.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009669.1

ORF Insert Sequence:

ATGGCCGAGT CGGTGGAGCG GCTGCTGCAG CGGGTTGAGG AGCTGGAGCA GGAACTCGCC 
CGGGAGAGAA GCCGGCGGAT TGCGGGGGAC GGCCACTGCG GCCGGACCCG CATCCAGAAG 
ATGAGCGACG AGGTGGTGGA TTCCAATCCC TACAGCCGTC TGATGGCATT GAAACGGATG 
GGAGTTGTCA GTGACTATGA GAAAATCCGT ACCTATGCTG TAGCAATAGT AGGTGTTGGT 
GGAGTTGGTA GTGTGACTGC TGAAATGCTG ACAAGATGTG GCATTGGTAA GTTGCTACTC 
TTTGACTATG ACAAGGTGGA GCTGGCCAAC ATGAATCGAC TTTTCTTCCA GCCGTATCAA 
GCAGGGATGA GTAAGGTCCA AGCAGCAGAG CACACCCTGA GGAGCATTAA CCCTGATGTT 
CTGTTTGAAG TTCACAACTA TAATATCACC ACCGTGGAAC ACTTCGAACA TTTCATGAAT 
AGAATAAGTA ATGGGGGATT AGAAGAAGGA CAACCTGTTG ACCTAGTTCT TAGCTGTGTG 
GACAACTTTG AAGCTCGAAT GGCAATAAAT ACAGCTTGTA ATGAACTTGG TCAGACATGG 
ATGGAATCTG GGGTCAGTGA AAACGCAGTT TCCGGGCACA TCCAGCTTAT GGTCCCTGGA 
GAGTCGGCTT GCTTTGCGTG CGCACCACCA CTTGTAGTTG CTTCAAACAT TGATGAAAAA 
ACCCTGAAAC GAGAAGGCGT CTGCGCGGCC AGCCTCCCCA CCACCATGGG AGTGGTTGCA 
GGGATCTTGG TACAAAATGT ATTAAAGTTT CTGTTAAAAT TTGGGACTGT CAGTTTTTAC 
CTCGGATACA ATGCCATGCA GGACTTCTTC CCTACCATGT TCATGAAGCC AAACCCTCAG 
TGTGATGACA AAAACTGTAG GAAGCAACAA GAAGAATACA AGAAGAGGGC ACCAGCGCAG 
CCCACGCAGG AGACTGCGCC CCAAGAGGAG GAAGAGGTAG TTCATGAGGA CAACGAGTGG 
GGTATTGAGC TGGTGTCTGA GGTTTCAGAA GAGGAACTGA AGAACTCGTC AGGTCCTGTT 
CCCACCTTAC CTGAAGGAAT TACAGTGGCA TACACAGTCC CAAAGAAGAG AGAGGATTCT 
GTTTCTGAAG TAACGGTGGA AGATTCTGGT GAAAGCTTGG AAGATCTCAT GGCCAGGATG 
AAGAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba5 ( XM_017595592.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451081.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62394
Clone ID Related Accession (Same CDS sequence)	XM_017595592.1
Accession Version	XM_017595592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCGAC TTTTCTTCCA GCCGTATCAA GCAGGGATGA GTAAGGTCCA AGCAGCAGAG 
CACACCCTGA GGAGCATTAA CCCTGATGTT CTGTTTGAAG TTCACAACTA TAATATCACC 
ACCGTGGAAC ACTTCGAACA TTTCATGAAT AGAATAAGTA ATGGGGGATT AGAAGAAGGA 
CAACCTGTTG ACCTAGTTCT TAGCTGTGTG GACAACTTTG AAGCTCGAAT GGCAATAAAT 
ACAGCTTGTA ATGAACTTGG TCAGACATGG ATGGAATCTG GGGTCAGTGA AAACGCAGTT 
TCCGGGCACA TCCAGCTTAT GGTCCCTGGA GAGTCGGCTT GCTTTGCGTG CGCACCACCA 
CTTGTAGTTG CTTCAAACAT TGATGAAAAA ACCCTGAAAC GAGAAGGCGT CTGCGCGGCC 
AGCCTCCCCA CCACCATGGG AGTGGTTGCA GGGATCTTGG TACAAAATGT ATTAAAGTTT 
CTGTTAAAAT TTGGGACTGT CAGTTTTTAC CTCGGATACA ATGCCATGCA GGACTTCTTC 
CCTACCATGT TCATGAAGCC AAACCCTCAG TGTGATGACA AAAACTGTAG GAAGCAACAA 
GAAGAATACA AGAAGAGGGC ACCAGCGCAG CCCACGCAGG AGACTGCGCC CCAAGAGGAG 
GAAGAGGTAG TTCATGAGGA CAACGAGTGG GGTATTGAGC TGGTGTCTGA GGTTTCAGAA 
GAGGAACTGA AGAACTCGTC AGGTCCTGTT CCCACCTTAC CTGAAGGAAT TACAGTGGCA 
TACACAGTCC CAAAGAAGAG AGAGGATTCT GTTTCTGAAG TAACGGTGGA AGATTCTGGT 
GAAAGCTTGG AAGATCTCAT GGCCAGGATG AAGAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451081.1
CDS	107..988
Translation	MNRLFFQPYQAGMSKVQAAEHTLRSINPDVLFEVHNYNITTVEHFEHFMNRISNGGLEEGQPVDLVLSCVDNFEARMAINTACNELGQTWMESGVSENAVSGHIQLMVPGESACFACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYNAMQDFFPTMFMKPNPQCDDKNCRKQQEEYKKRAPAQPTQETAPQEEEEVVHEDNEWGIELVSEVSEEELKNSSGPVPTLPEGITVAYTVPKKREDSVSEVTVEDSGESLEDLMARMKKM

Target ORF information:

RefSeq Version	XM_017595592.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 5 (Uba5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595592.1

ORF Insert Sequence:

ATGAATCGAC TTTTCTTCCA GCCGTATCAA GCAGGGATGA GTAAGGTCCA AGCAGCAGAG 
CACACCCTGA GGAGCATTAA CCCTGATGTT CTGTTTGAAG TTCACAACTA TAATATCACC 
ACCGTGGAAC ACTTCGAACA TTTCATGAAT AGAATAAGTA ATGGGGGATT AGAAGAAGGA 
CAACCTGTTG ACCTAGTTCT TAGCTGTGTG GACAACTTTG AAGCTCGAAT GGCAATAAAT 
ACAGCTTGTA ATGAACTTGG TCAGACATGG ATGGAATCTG GGGTCAGTGA AAACGCAGTT 
TCCGGGCACA TCCAGCTTAT GGTCCCTGGA GAGTCGGCTT GCTTTGCGTG CGCACCACCA 
CTTGTAGTTG CTTCAAACAT TGATGAAAAA ACCCTGAAAC GAGAAGGCGT CTGCGCGGCC 
AGCCTCCCCA CCACCATGGG AGTGGTTGCA GGGATCTTGG TACAAAATGT ATTAAAGTTT 
CTGTTAAAAT TTGGGACTGT CAGTTTTTAC CTCGGATACA ATGCCATGCA GGACTTCTTC 
CCTACCATGT TCATGAAGCC AAACCCTCAG TGTGATGACA AAAACTGTAG GAAGCAACAA 
GAAGAATACA AGAAGAGGGC ACCAGCGCAG CCCACGCAGG AGACTGCGCC CCAAGAGGAG 
GAAGAGGTAG TTCATGAGGA CAACGAGTGG GGTATTGAGC TGGTGTCTGA GGTTTCAGAA 
GAGGAACTGA AGAACTCGTC AGGTCCTGTT CCCACCTTAC CTGAAGGAAT TACAGTGGCA 
TACACAGTCC CAAAGAAGAG AGAGGATTCT GTTTCTGAAG TAACGGTGGA AGATTCTGGT 
GAAAGCTTGG AAGATCTCAT GGCCAGGATG AAGAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
American journal of human genetics99(3)695-703(2016 09)
Colin E,Daniel J,Ziegler A,Wakim J,Scrivo A,Haack TB,Khiati S,Denommé AS,Amati-Bonneau P,Charif M,Procaccio V,Reynier P,Aleck KA,Botto LD,Herper CL,Kaiser CS,Nabbout R,N'Guyen S,Mora-Lorca JA,Assmann B,Christ S,Meitinger T,Strom TM,Prokisch H,,Miranda-Vizuete A,Hoffmann GF,Lenaers G,Bomont P,Liebau E,Bonneau D

UBA5 Mutations Cause a New Form of Autosomal Recessive Cerebellar Ataxia.
PloS one11(2)e0149039(2016)
Duan R,Shi Y,Yu L,Zhang G,Li J,Lin Y,Guo J,Wang J,Shen L,Jiang H,Wang G,Tang B

Modification of ASC1 by UFM1 is crucial for ERα transactivation and breast cancer development.
Molecular cell56(2)261-274(2014 Oct)
Yoo HM,Kang SH,Kim JY,Lee JE,Seong MW,Lee SW,Ka SH,Sou YS,Komatsu M,Tanaka K,Lee ST,Noh DY,Baek SH,Jeon YJ,Chung CH

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
PloS one7(11)e48587(2012)
Zhang Y,Zhang M,Wu J,Lei G,Li H

A novel type of E3 ligase for the Ufm1 conjugation system.
The Journal of biological chemistry285(8)5417-27(2010 Feb)
Tatsumi K,Sou YS,Tada N,Nakamura E,Iemura S,Natsume T,Kang SH,Chung CH,Kasahara M,Kominami E,Yamamoto M,Tanaka K,Komatsu M

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Uba5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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