ZGRF1 gene cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

mRNA	Protein	Name
XM_012179475.2	XP_012034865.1	protein ZGRF1 isoform X3
XM_012179482.2	XP_012034872.1	protein ZGRF1 isoform X7
XM_012143087.2	XP_011998477.1	protein ZGRF1 isoform X4
XM_012143093.2	XP_011998483.1	protein ZGRF1 isoform X7
XM_012179473.2	XP_012034863.1	protein ZGRF1 isoform X1
XM_012179473.3	XP_012034863.2	LOW QUALITY PROTEIN: protein ZGRF1
XM_012179479.2	XP_012034869.1	protein ZGRF1 isoform X5
XM_012143085.2	XP_011998475.1	protein ZGRF1 isoform X3
XM_012143092.2	XP_011998482.1	protein ZGRF1 isoform X6
XM_012143089.2	XP_011998479.1	protein ZGRF1 isoform X5
XM_012179474.2	XP_012034864.1	protein ZGRF1 isoform X2
XM_012179477.2	XP_012034867.1	protein ZGRF1 isoform X4
XM_012179481.2	XP_012034871.1	protein ZGRF1 isoform X6
XM_012143084.2	XP_011998474.1	protein ZGRF1 isoform X2
XM_012143083.2	XP_011998473.1	protein ZGRF1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv21661	XM_012143092.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv21662	XM_012143093.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm39515	XM_012179481.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm39516	XM_012179482.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv21656	XM_012143083.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv21657	XM_012143084.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv21658	XM_012143085.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv21659	XM_012143087.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv21660	XM_012143089.2 Latest version!	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm39507	XM_012179473.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm39508	XM_012179474.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm39509	XM_012179475.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm39511	XM_012179477.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm39513	XM_012179479.2 Latest version!	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm81707	XM_012179473.3 Latest version!	Ovis aries zinc finger GRF-type containing 1 (ZGRF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ZGRF1 ( XM_012143092.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998482.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOv21661
Clone ID Related Accession (Same CDS sequence)	XM_012143092.2
Accession Version	XM_012143092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG AGGTAAATGA CTCTGAGCTA TGCTTCCCAA GTGGACAGAA AGTAAAATCT 
GCATGTCTTC CCCAAAGGCA AATTCACATA CCAGCTGTTT TTCAGTCTCC TGCTCATTAT 
AAGCAGATTT TTACATCTTG TCTCACAGAA CATTTAAATA TATTGCTATT TGGATTGGCA 
CAAAGGTTGC ACAAAGCTCT TTCAAAAGTT GACATATCAT TTTACACATC ATCAGATGGA 
GACAAACTGA AAAATGTAGA CAATAATGTG CCATCCTGCC ATCATCAGCA GCCTGCAAAA 
CTGGTCATGG TGAAAAAGGA AGGTCCAAAT AAGGGTCGTC TCTTTTACGC CTGTGATGGA 
CCCAAAGCTA ACCAATGTAA GTTTTTTAAG TGGTTTGAAG AAATGACCCC AGGATACTCA 
ACACAGGAAA AATCTCTACC TAGCATGGTT TTAAGTGATA CAAAAAGTAT TGGCTTCTAC 
TTAAGAAGTC AAAACATACC CCTCTATGAG GAATGCCAAC TTTTCGTGAG AAAAGGATTT 
GATTTTCAAA GAAAACGGTA CGGTAAACTA AAGAAGTTTA CTACTGTAGA TCCTGAATTT 
TATAATGAAC CTAAGAGCAA ACTTTATCTT AAACTGAGTC GGAAAGAAAG TTCATCTGCT 
TACAGCAAAG ATGATCTTTG GGTGGTTTCA AAAACCCTAG ACTTTGAATT GGATACTTTT 
ATTGCATGTA GTACTTTCTT TGGACCATCA TCTATCAATG AGGTAGAGCT TCTACCTTTG 
AAAGGTTATT TCCCTTCAAA CTGGCCCACT AACACAGTGG TCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAGTGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGCGTTTTT 
GGAGCAGGAA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA CGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCAAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAAGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998482.1
CDS	249..6173
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143092.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143092.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG AGGTAAATGA CTCTGAGCTA TGCTTCCCAA GTGGACAGAA AGTAAAATCT 
GCATGTCTTC CCCAAAGGCA AATTCACATA CCAGCTGTTT TTCAGTCTCC TGCTCATTAT 
AAGCAGATTT TTACATCTTG TCTCACAGAA CATTTAAATA TATTGCTATT TGGATTGGCA 
CAAAGGTTGC ACAAAGCTCT TTCAAAAGTT GACATATCAT TTTACACATC ATCAGATGGA 
GACAAACTGA AAAATGTAGA CAATAATGTG CCATCCTGCC ATCATCAGCA GCCTGCAAAA 
CTGGTCATGG TGAAAAAGGA AGGTCCAAAT AAGGGTCGTC TCTTTTACGC CTGTGATGGA 
CCCAAAGCTA ACCAATGTAA GTTTTTTAAG TGGTTTGAAG AAATGACCCC AGGATACTCA 
ACACAGGAAA AATCTCTACC TAGCATGGTT TTAAGTGATA CAAAAAGTAT TGGCTTCTAC 
TTAAGAAGTC AAAACATACC CCTCTATGAG GAATGCCAAC TTTTCGTGAG AAAAGGATTT 
GATTTTCAAA GAAAACGGTA CGGTAAACTA AAGAAGTTTA CTACTGTAGA TCCTGAATTT 
TATAATGAAC CTAAGAGCAA ACTTTATCTT AAACTGAGTC GGAAAGAAAG TTCATCTGCT 
TACAGCAAAG ATGATCTTTG GGTGGTTTCA AAAACCCTAG ACTTTGAATT GGATACTTTT 
ATTGCATGTA GTACTTTCTT TGGACCATCA TCTATCAATG AGGTAGAGCT TCTACCTTTG 
AAAGGTTATT TCCCTTCAAA CTGGCCCACT AACACAGTGG TCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAGTGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGCGTTTTT 
GGAGCAGGAA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA CGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCAAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAAGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143093.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998483.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

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CloneID	OOv21662
Clone ID Related Accession (Same CDS sequence)	XM_012143093.2
Accession Version	XM_012143093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG CTGGGAAACC ATTGACTACA 
ATTGTTTCAC AAGGTATACC AAAGTCTCCT CATCTGAACC AGGATCCTCA TCAGATGATA 
AAAGAAAATG AAGCTGAACC AAGTGAATCA CTTCAGTCTT TGCAGTTCTT TCCCTTGGGC 
ACTGAAGAAA AGACTGCTTT TCTAGCTGTT ATTCCTAAAC AAGTGGAGAG AGAAGCCGGT 
AAAGACCCCA CGCCTGTTGA GTTTCAAGGG CACCAAGTGA AAGGATCTGC TTCTAGCACT 
GTGGTGGTCA GAGGACACAG CTCGCAGGTC GAATGCAATC AGGTTTTGGA TGGCACTGTG 
TATGAAAGCC GCACAACAAA CGCTTGTTCT TTGACACCAG CGTTGCCTAG CACTTGTATG 
CAGACTGACT TCTTGCAGTT TCCAAATGAA GAAAACTTTC CAAAAGGAGA AACTAGCTTT 
ACTATTCAAG ACAATTTTCA AGTGAGAGCT GAGTTTAATA AGGACTTGAC TAAAAATGAT 
TCAATTTTAG ATTTCCCTCC TAAAGATTCA GACCATTTAT ATAATTATTT GGATAATAAT 
TTTAAATCAA CAGAGAGGAC TTCGTGGGAA AAAAATAAGG TGATGTCACC AGAACAGAAG 
ATCTCAACAT TAAGCCCTGT TTCTACTTTA TCTTTTAACT CAAGAGATGA TGACTTCATG 
CTAGAATTCT CTGAGGAGTC CCTGAAAACG CAAACTTTAC CTGTAATGAA AAAATCAAGT 
TCTCTGGAGA ATAAGAAGAA CCTTCAAAGG CTTTCCTTTG AAGTAAGTAG AACCCGAGCT 
CCACTTGTTA CTTTGCCACC TGCCAGTGGG CCACCTGACT TAGATTCTTG TTCATTTATG 
ATGGACCGTG ACAGGCAAAA GTCTTCCGGT TCTCCCATAT TCAGCCTGTG TGAAGAGTCA 
GCAGTTCCTT CTTTTAGCTT TGGGCCCAAG GACCAAAGTG AAGCTTCTTT CTCTAACGAA 
TCTAGGGAAG TGACTCCAAG TGATGTTTCA CTTCTTGATA ATACATCTAC TCAAAGCAAG 
TGGCTGAAAT ATCAAAATAT ACGCCAGTGC AACTTGACTG CTCCAGACAG AGTTGATGAG 
AAAGTAATAG ATGGCTTCTT TGCTGAGGCT GTTCCTGGGA TGCATTTTAG TAACACAGGT 
GAAAGAGAGA ATGATGCTGT GAATGAAAGT TCTTTAGACT CTGTGCATTT ACAAATGATG 
AAACACATGC TTCAACAGCA GCAGCAGGAT TTTAGCAGTC AAGATTCGGT TTGCAGAAAG 
AAGGCATTTT CTTTGAATGT AAACGAGGCT TCTAAGACCG AGGAAATTCA GAATTTGTTA 
GGAGAGTATG CCTACTACAA CTGCAGTCTA ACAGGAGGTT TACAGGAGGT AAATGACTCT 
GAGCTATGCT TCCCAAGTGG ACAGAAAGTA AAATCTGCAT GTCTTCCCCA AAGGCAAATT 
CACATACCAG CTGTTTTTCA GTCTCCTGCT CATTATAAGC AGATTTTTAC ATCTTGTCTC 
ACAGAACATT TAAATATATT GCTATTTGGA TTGGCACAAA GGTTGCACAA AGCTCTTTCA 
AAAGTTGACA TATCATTTTA CACATCATCA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTACGCCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TACTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATACAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACGGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGTCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AGTGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGC GTTTTTGGAG CAGGAAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTACGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCA AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAAGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998483.1
CDS	249..5309
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143093.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143093.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG CTGGGAAACC ATTGACTACA 
ATTGTTTCAC AAGGTATACC AAAGTCTCCT CATCTGAACC AGGATCCTCA TCAGATGATA 
AAAGAAAATG AAGCTGAACC AAGTGAATCA CTTCAGTCTT TGCAGTTCTT TCCCTTGGGC 
ACTGAAGAAA AGACTGCTTT TCTAGCTGTT ATTCCTAAAC AAGTGGAGAG AGAAGCCGGT 
AAAGACCCCA CGCCTGTTGA GTTTCAAGGG CACCAAGTGA AAGGATCTGC TTCTAGCACT 
GTGGTGGTCA GAGGACACAG CTCGCAGGTC GAATGCAATC AGGTTTTGGA TGGCACTGTG 
TATGAAAGCC GCACAACAAA CGCTTGTTCT TTGACACCAG CGTTGCCTAG CACTTGTATG 
CAGACTGACT TCTTGCAGTT TCCAAATGAA GAAAACTTTC CAAAAGGAGA AACTAGCTTT 
ACTATTCAAG ACAATTTTCA AGTGAGAGCT GAGTTTAATA AGGACTTGAC TAAAAATGAT 
TCAATTTTAG ATTTCCCTCC TAAAGATTCA GACCATTTAT ATAATTATTT GGATAATAAT 
TTTAAATCAA CAGAGAGGAC TTCGTGGGAA AAAAATAAGG TGATGTCACC AGAACAGAAG 
ATCTCAACAT TAAGCCCTGT TTCTACTTTA TCTTTTAACT CAAGAGATGA TGACTTCATG 
CTAGAATTCT CTGAGGAGTC CCTGAAAACG CAAACTTTAC CTGTAATGAA AAAATCAAGT 
TCTCTGGAGA ATAAGAAGAA CCTTCAAAGG CTTTCCTTTG AAGTAAGTAG AACCCGAGCT 
CCACTTGTTA CTTTGCCACC TGCCAGTGGG CCACCTGACT TAGATTCTTG TTCATTTATG 
ATGGACCGTG ACAGGCAAAA GTCTTCCGGT TCTCCCATAT TCAGCCTGTG TGAAGAGTCA 
GCAGTTCCTT CTTTTAGCTT TGGGCCCAAG GACCAAAGTG AAGCTTCTTT CTCTAACGAA 
TCTAGGGAAG TGACTCCAAG TGATGTTTCA CTTCTTGATA ATACATCTAC TCAAAGCAAG 
TGGCTGAAAT ATCAAAATAT ACGCCAGTGC AACTTGACTG CTCCAGACAG AGTTGATGAG 
AAAGTAATAG ATGGCTTCTT TGCTGAGGCT GTTCCTGGGA TGCATTTTAG TAACACAGGT 
GAAAGAGAGA ATGATGCTGT GAATGAAAGT TCTTTAGACT CTGTGCATTT ACAAATGATG 
AAACACATGC TTCAACAGCA GCAGCAGGAT TTTAGCAGTC AAGATTCGGT TTGCAGAAAG 
AAGGCATTTT CTTTGAATGT AAACGAGGCT TCTAAGACCG AGGAAATTCA GAATTTGTTA 
GGAGAGTATG CCTACTACAA CTGCAGTCTA ACAGGAGGTT TACAGGAGGT AAATGACTCT 
GAGCTATGCT TCCCAAGTGG ACAGAAAGTA AAATCTGCAT GTCTTCCCCA AAGGCAAATT 
CACATACCAG CTGTTTTTCA GTCTCCTGCT CATTATAAGC AGATTTTTAC ATCTTGTCTC 
ACAGAACATT TAAATATATT GCTATTTGGA TTGGCACAAA GGTTGCACAA AGCTCTTTCA 
AAAGTTGACA TATCATTTTA CACATCATCA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTACGCCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TACTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATACAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACGGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGTCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AGTGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGC GTTTTTGGAG CAGGAAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTACGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCA AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAAGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179481.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034871.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

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CloneID	OSm39515
Clone ID Related Accession (Same CDS sequence)	XM_012179481.2
Accession Version	XM_012179481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG AGGTAAATGA CTCTGAGCTA TGCTTCCCAA GTGGACAGAA AGTAAAATCT 
GCATGTCTTC CCCAAAGGCA AATTCACATA CCAGCTGTTT TTCAGTCTCC TGCTCATTAT 
AAGCAGATTT TTACATCTTG TCTCACAGAA CATTTAAATA TATTGCTATT TGGATTGGCA 
CAAAGGTTGC ACAAAGCTCT TTCAAAAGTT GACATATCAT TTTACACATC ATCAGATGGA 
GACAAACTGA AAAATGTAGA CAATAATGTG CCATCCTGCC ATCATCAGCA GCCTGCAAAA 
CTGGTCATGG TGAAAAAGGA AGGTCCAAAT AAGGGTCGTC TCTTTTATAC CTGTGATGGA 
CCCAAAGCTA ACCAATGTAA GTTTTTTAAG TGGTTTGAAG AAATGACCCC AGGATATTCA 
ACACAGGAAA AATCTCTACC TAGCATGGTT TTAAGTGATA TAAAAAGTAT TGGCTTCTAC 
TTAAGAAGTC AAAACATACC CCTCTATGAG GAATGCCAAC TTTTCGTGAG AAAAGGATTT 
GATTTTCAAA GAAAACAGTA CGGTAAACTA AAGAAGTTTA CTACTGTAGA TCCTGAATTT 
TATAATGAAC CTAAGAGCAA ACTTTATCTT AAACTGAGTC GGAAAGAAAG TTCATCTGCT 
TACAGCAAAG ATGATCTTTG GGTGGTTTCA AAAACCCTAG ACTTTGAATT GGATACTTTT 
ATTGCATGTA GTACTTTCTT TGGACCATCA TCTATCAATG AGGTAGAGCT TCTACCTTTG 
AAAGGTTATT TCCCTTCAAA CTGGCCCACT AACACAGTGG CCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAATGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGTGTTTTC 
GGAGCAGGGA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA TGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCCAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAGGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034871.1
CDS	251..6175
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179481.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179481.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG AGGTAAATGA CTCTGAGCTA TGCTTCCCAA GTGGACAGAA AGTAAAATCT 
GCATGTCTTC CCCAAAGGCA AATTCACATA CCAGCTGTTT TTCAGTCTCC TGCTCATTAT 
AAGCAGATTT TTACATCTTG TCTCACAGAA CATTTAAATA TATTGCTATT TGGATTGGCA 
CAAAGGTTGC ACAAAGCTCT TTCAAAAGTT GACATATCAT TTTACACATC ATCAGATGGA 
GACAAACTGA AAAATGTAGA CAATAATGTG CCATCCTGCC ATCATCAGCA GCCTGCAAAA 
CTGGTCATGG TGAAAAAGGA AGGTCCAAAT AAGGGTCGTC TCTTTTATAC CTGTGATGGA 
CCCAAAGCTA ACCAATGTAA GTTTTTTAAG TGGTTTGAAG AAATGACCCC AGGATATTCA 
ACACAGGAAA AATCTCTACC TAGCATGGTT TTAAGTGATA TAAAAAGTAT TGGCTTCTAC 
TTAAGAAGTC AAAACATACC CCTCTATGAG GAATGCCAAC TTTTCGTGAG AAAAGGATTT 
GATTTTCAAA GAAAACAGTA CGGTAAACTA AAGAAGTTTA CTACTGTAGA TCCTGAATTT 
TATAATGAAC CTAAGAGCAA ACTTTATCTT AAACTGAGTC GGAAAGAAAG TTCATCTGCT 
TACAGCAAAG ATGATCTTTG GGTGGTTTCA AAAACCCTAG ACTTTGAATT GGATACTTTT 
ATTGCATGTA GTACTTTCTT TGGACCATCA TCTATCAATG AGGTAGAGCT TCTACCTTTG 
AAAGGTTATT TCCCTTCAAA CTGGCCCACT AACACAGTGG CCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAATGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGTGTTTTC 
GGAGCAGGGA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA TGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCCAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAGGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179482.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034872.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

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CloneID	OSm39516
Clone ID Related Accession (Same CDS sequence)	XM_012179482.2
Accession Version	XM_012179482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG CTGGGAAACC ATTGACTACA 
ATTGTTTCAC AAGCTATACC AAAGTCTCCT CATCTGAACC AGGATCCTCA TCAGATGATA 
AAAGAAAATG AAGCTGAACC AAGTGAATCA CTTCAGTCTT TGCAGTTCTT TCCCTTGGGC 
ACTGAAGAAA AGACTGCTTT TCTAGCTGTT ATTCCTAAAC AAGTGGAGAG AGAAGCCGGT 
AAAGACCCCG CGCCTGTTGA GTTTCAAGGG CACCAAGTGA AAGGATCTGC TTCTAGCACT 
GTGGTGGTCA GAGGACACAG CTCGCAGGTC GAATGCAATC AGGTTTTGGA TGGCACTGTG 
TATGAAAGCC GCACAACAAA CGCTTGTTCT TTGACACCAG CGTTGCCTAG CACTTGTATG 
CAGACTGACT TCTTGCAGTT TCCAAATGAA GAAAACTTTC CAAAAGGAGA AACTAGCTTT 
ACTATTCAAG ACAATTTTCA AGTGAGAGCT GAGTTTAATA AGGACTTGAC TAAAAATGAT 
TCAATTTTAG ATTTCCCTCC TAAAGATTCA GACCATTTAT ATAATTATTT GGATAATAAT 
TTTAAATCAA CAGAGAGGAC TTCGTGGGAA AAAAATAAGG TGATGTCACC AGAACAGAAG 
ATCTCAACAT TAAGCCCTGT TTCTACTTTA TCTTTTAACT CAAGAGATGA TGACTTCATG 
CTAGAATTCT CTGAGGAGTC CCTGAAAACG CAAACTTTAC CTGTAATGAA AAAACCAAGT 
TCTCTGGAGA ATAAGAAGAA CCTTCAAAGG CTTTCCTTTG AAGTAAGTAG AACCCGAGCT 
CCACTTGTTA CTTTGCCACC TGCCAGTGGG CCACCTGACT TAGATTCTTG TTCATTTATG 
ATGGACTGTG ACAGGCAAAA GTCTTCCGGT TCTCCCATAT TCAGCCTGTG TGAAGAGTCA 
GCAGTTCCTT CTTTTAGCTT TGGGCCCAAG GACCAAAGTG AAGCTTCTTT CTCTAACGAA 
TCTAGGGAAG TGACTCCAAG TGATGTTTCA CTTCTTGATA ATACATCTAC TCAAAGCAAG 
TGGCTGAAAT ATCAAAATAT ACGCCAGTGC AACTTGACTG CTCCAGACAG AGTTGATGAG 
AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTCCTGGGA CGCATTTTAG TAACACAGGT 
GAAAGAGAGA ATGATGCTGT GAATGAAAGT TCTTTAGACT CTGTGCATTT ACAAATGATG 
AAACACATGC TTCTACAGCA GCAGCAGGAT TTTAGCAGTC AAGATTCGGT TTGCAGAAAG 
AAGGCATTTT CTTTGAATGT AAACGAGGCT TCTAAGACCG AGGAAATTCA GAATTTGTTA 
GGAGAGTATG CCTACTACAA CTGCAGTCTA ACAGGAGGTT TACAGGAGGT AAATGACTCT 
GAGCTATGCT TCCCAAGTGG ACAGAAAGTA AAATCTGCAT GTCTTCCCCA AAGGCAAATT 
CACATACCAG CTGTTTTTCA GTCTCCTGCT CATTATAAGC AGATTTTTAC ATCTTGTCTC 
ACAGAACATT TAAATATATT GCTATTTGGA TTGGCACAAA GGTTGCACAA AGCTCTTTCA 
AAAGTTGACA TATCATTTTA CACATCATCA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTATACCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TATTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATATAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACAGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGCCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AATGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGT GTTTTCGGAG CAGGGAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTATGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCC AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAGGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034872.1
CDS	251..5311
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179482.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179482.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG CTGGGAAACC ATTGACTACA 
ATTGTTTCAC AAGCTATACC AAAGTCTCCT CATCTGAACC AGGATCCTCA TCAGATGATA 
AAAGAAAATG AAGCTGAACC AAGTGAATCA CTTCAGTCTT TGCAGTTCTT TCCCTTGGGC 
ACTGAAGAAA AGACTGCTTT TCTAGCTGTT ATTCCTAAAC AAGTGGAGAG AGAAGCCGGT 
AAAGACCCCG CGCCTGTTGA GTTTCAAGGG CACCAAGTGA AAGGATCTGC TTCTAGCACT 
GTGGTGGTCA GAGGACACAG CTCGCAGGTC GAATGCAATC AGGTTTTGGA TGGCACTGTG 
TATGAAAGCC GCACAACAAA CGCTTGTTCT TTGACACCAG CGTTGCCTAG CACTTGTATG 
CAGACTGACT TCTTGCAGTT TCCAAATGAA GAAAACTTTC CAAAAGGAGA AACTAGCTTT 
ACTATTCAAG ACAATTTTCA AGTGAGAGCT GAGTTTAATA AGGACTTGAC TAAAAATGAT 
TCAATTTTAG ATTTCCCTCC TAAAGATTCA GACCATTTAT ATAATTATTT GGATAATAAT 
TTTAAATCAA CAGAGAGGAC TTCGTGGGAA AAAAATAAGG TGATGTCACC AGAACAGAAG 
ATCTCAACAT TAAGCCCTGT TTCTACTTTA TCTTTTAACT CAAGAGATGA TGACTTCATG 
CTAGAATTCT CTGAGGAGTC CCTGAAAACG CAAACTTTAC CTGTAATGAA AAAACCAAGT 
TCTCTGGAGA ATAAGAAGAA CCTTCAAAGG CTTTCCTTTG AAGTAAGTAG AACCCGAGCT 
CCACTTGTTA CTTTGCCACC TGCCAGTGGG CCACCTGACT TAGATTCTTG TTCATTTATG 
ATGGACTGTG ACAGGCAAAA GTCTTCCGGT TCTCCCATAT TCAGCCTGTG TGAAGAGTCA 
GCAGTTCCTT CTTTTAGCTT TGGGCCCAAG GACCAAAGTG AAGCTTCTTT CTCTAACGAA 
TCTAGGGAAG TGACTCCAAG TGATGTTTCA CTTCTTGATA ATACATCTAC TCAAAGCAAG 
TGGCTGAAAT ATCAAAATAT ACGCCAGTGC AACTTGACTG CTCCAGACAG AGTTGATGAG 
AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTCCTGGGA CGCATTTTAG TAACACAGGT 
GAAAGAGAGA ATGATGCTGT GAATGAAAGT TCTTTAGACT CTGTGCATTT ACAAATGATG 
AAACACATGC TTCTACAGCA GCAGCAGGAT TTTAGCAGTC AAGATTCGGT TTGCAGAAAG 
AAGGCATTTT CTTTGAATGT AAACGAGGCT TCTAAGACCG AGGAAATTCA GAATTTGTTA 
GGAGAGTATG CCTACTACAA CTGCAGTCTA ACAGGAGGTT TACAGGAGGT AAATGACTCT 
GAGCTATGCT TCCCAAGTGG ACAGAAAGTA AAATCTGCAT GTCTTCCCCA AAGGCAAATT 
CACATACCAG CTGTTTTTCA GTCTCCTGCT CATTATAAGC AGATTTTTAC ATCTTGTCTC 
ACAGAACATT TAAATATATT GCTATTTGGA TTGGCACAAA GGTTGCACAA AGCTCTTTCA 
AAAGTTGACA TATCATTTTA CACATCATCA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTATACCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TATTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATATAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACAGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGCCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AATGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGT GTTTTCGGAG CAGGGAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTATGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCC AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAGGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143083.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998473.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv21656
Clone ID Related Accession (Same CDS sequence)	XM_012143083.2
Accession Version	XM_012143083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA AGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998473.1
CDS	249..6776
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143083.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143083.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA AGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143084.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998474.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

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CloneID	OOv21657
Clone ID Related Accession (Same CDS sequence)	XM_012143084.2
Accession Version	XM_012143084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACATGG TCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAGTGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGCGTTTTT 
GGAGCAGGAA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA CGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCAAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAAGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998474.1
CDS	249..6773
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNMVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143084.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143084.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACATGG TCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAGTGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGCGTTTTT 
GGAGCAGGAA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA CGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCAAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAAGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143085.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998475.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

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CloneID	OOv21658
Clone ID Related Accession (Same CDS sequence)	XM_012143085.2
Accession Version	XM_012143085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA AGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998475.1
CDS	249..6716
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143085.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143085.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA AGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143087.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998477.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

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CloneID	OOv21659
Clone ID Related Accession (Same CDS sequence)	XM_012143087.2
Accession Version	XM_012143087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTACGCCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TACTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATACAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACGGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGTCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AGTGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGC GTTTTTGGAG CAGGAAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTACGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCA AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAAGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998477.1
CDS	249..6689
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGKEGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012143087.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143087.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTACGCCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TACTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATACAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACGGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGTCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AGTGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGC GTTTTTGGAG CAGGAAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTACGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCA AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAAGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012143089.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011998479.1 Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

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CloneID	OOv21660
Clone ID Related Accession (Same CDS sequence)	XM_012143089.2
Accession Version	XM_012143089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein ZGRF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942500.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012143089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG GAAGGAAGGT GGATTGCAGC 
ATGCAAGCCA GTGTGAACCA CAACTGGACC TTCTCCTTAA AGATTATCTT GAAAAACAAG 
CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011998479.1
CDS	249..6590
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCESISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQGIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVIDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKRYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCGRKVDCSMQASVNHNWTFSLKIILKNKQKKNRRKRLKKIN

Target ORF information:

RefSeq Version	XM_012143089.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012143089.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAG TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG GTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAATAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACG GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTACGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG GAAGGAAGGT GGATTGCAGC 
ATGCAAGCCA GTGTGAACCA CAACTGGACC TTCTCCTTAA AGATTATCTT GAAAAACAAG 
CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179473.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034863.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

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CloneID	OSm39507
Clone ID Related Accession (Same CDS sequence)	XM_012179473.2
Accession Version	XM_012179473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034863.1
CDS	251..6778
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179473.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179473.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179474.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034864.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

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CloneID	OSm39508
Clone ID Related Accession (Same CDS sequence)	XM_012179474.2
Accession Version	XM_012179474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACATGG CCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAATGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGTGTTTTC 
GGAGCAGGGA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA TGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCCAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAGGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034864.1
CDS	251..6775
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNMAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179474.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179474.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACATGG CCCACGCCTT ATTGGTTTGT 
AATGCCAGCA CAGAGCTGAC CACTTTAAAG AATATTCGTG ACTACTTTAA TCCAGCTACT 
CTGCCTCTAA CACAGTACCT GATAGCAATG TCTTCATCAA CTACAATGAA CAACAAGAGA 
GTCAATAAGA GAAAGTTTAT CCCACCAGCC TTCTCAAATA TCAACACAAA ATTTGAACTA 
CTCAGTATAG AAGCAACATT GAAGTTGGCT AGTGAGTTAA TTCAGGCGCA CAAGTTAAAC 
AAAGATCAGG CTGCAGCTCT AATTCAGATA GCGCAAATGA TGGCATCACG CCAGAGTGTT 
GAAGAAGCGA AGGAACTGGC AACTCACACC CTCCCTATCA CGATCATCCA CGGTGTTTTC 
GGAGCAGGGA AGAGTTACTT GCTGGCCGTG GTGATTTTGT TTTTTGTGCA ACTATTTGAA 
AAATGTGAAG CTCTTACAGT TGGAAATGCA AGACCGTGGA AACTTCTAAT TTCTTCTTCT 
ACTAATGTAG CTGTTGATAG AGTGCTTCTT GGGCTTCTTA GTCTTGGATT TGAAAAGTTT 
GTCAGAGTTG GGAGTGTCAG AAAGATTGCC AAGCCAGTTT TACCTTACAG TTTGTATGCT 
GGCTCAGAAA ATGAAAATGA ACAATTAAAA GAACTACATG CACTCATGAA GGAAGACTTG 
ACCCCTGTGG AAAGAGTCTA TGTGAGGAAA AGTATTGAGC AGCATAAACT GGGGACCAAT 
AAAACCTTGC TGAAACAGGT TCGAGTAGTT GGAGTTACCT GTGCAGCCTG CCCGTTCCCA 
TGCATGAATG ATTTGAAGTT CCCTGTGGTT GTCCTGGATG AGTGTAGTCA AATAACAGAA 
CCTGCTTCTC TCCTTCCCAT TGCAAGGTTT GAGTGTGAAA AGCTGATACT TGTTGGAGAT 
CCCCAACAGC TTCCTCCTAC TATTCAGGGT TCTGATGCAG CTCACGAAAA TGGACTGGAA 
CAAACTCTTT TTGACCGACT CTGCTTAATG GGTCACAAGC CAATTCTGTT GAGAACTCAG 
TACCGTTGTC ACCCCACAAT CAGTGCTATT GCTAATGATC TGTTTTATGA AGGAAATCTC 
ATGAATGGCA TTTCCGAAAC AGAGAGGAGC CCTTTACTGG AATGGCTTCC AACTCTGTGT 
TTCTATAACG TAAAAGGACT GGAACAGATT GAGAGAGGTA ACAGCTTTCA TAATGTGGCA 
GAAGCTGCTT TTACACTCAA GTTGATTCAA TCACTGATTG CAAGTGGAAT AGCGGGCTCC 
ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTCTGTCA TCTACTCAGT 
GCTGCGGACT TCGACCATCC TAACGTGAAA GCTGTGCAGG TGTCCACAGT AGATGCTTTT 
CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGCGTAA GGACCAGACA AGTAGGATTT 
ATTGATTCAG AAAAAAGAAT GAATGTTGCA TTGACCAGAG GAAGGAGGCA TTTGTTGATT 
GTAGGAAATT TAGCCTGTCT GAGGAAAAAT CGACTATGGG GGCGTGTGAT CCAACACTGT 
GAAGGGAGGG AAGGTGGATT GCAGCATGCA AGCCAGTGTG AACCACAACT GGACCTTCTC 
CTTAAAGATT ATCTTGAAAA ACAAGCAGAA GAAAAACAGA AGAAAAAGGC TGAAAAAGAT 
AAATTGACAG ATAAAAAATC TTATTCACAA AAAGACATGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179475.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034865.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

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CloneID	OSm39509
Clone ID Related Accession (Same CDS sequence)	XM_012179475.2
Accession Version	XM_012179475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034865.1
CDS	251..6718
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179475.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179475.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAGT GTGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179477.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034867.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

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CloneID	OSm39511
Clone ID Related Accession (Same CDS sequence)	XM_012179477.2
Accession Version	XM_012179477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTATACCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TATTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATATAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACAGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGCCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AATGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGT GTTTTCGGAG CAGGGAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTATGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCC AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAGGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034867.1
CDS	251..6691
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQCEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179477.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179477.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GATGGAGACA AACTGAAAAA TGTAGACAAT 
AATGTGCCAT CCTGCCATCA TCAGCAGCCT GCAAAACTGG TCATGGTGAA AAAGGAAGGT 
CCAAATAAGG GTCGTCTCTT TTATACCTGT GATGGACCCA AAGCTAACCA ATGTAAGTTT 
TTTAAGTGGT TTGAAGAAAT GACCCCAGGA TATTCAACAC AGGAAAAATC TCTACCTAGC 
ATGGTTTTAA GTGATATAAA AAGTATTGGC TTCTACTTAA GAAGTCAAAA CATACCCCTC 
TATGAGGAAT GCCAACTTTT CGTGAGAAAA GGATTTGATT TTCAAAGAAA ACAGTACGGT 
AAACTAAAGA AGTTTACTAC TGTAGATCCT GAATTTTATA ATGAACCTAA GAGCAAACTT 
TATCTTAAAC TGAGTCGGAA AGAAAGTTCA TCTGCTTACA GCAAAGATGA TCTTTGGGTG 
GTTTCAAAAA CCCTAGACTT TGAATTGGAT ACTTTTATTG CATGTAGTAC TTTCTTTGGA 
CCATCATCTA TCAATGAGGT AGAGCTTCTA CCTTTGAAAG GTTATTTCCC TTCAAACTGG 
CCCACTAACA CAGTGGCCCA CGCCTTATTG GTTTGTAATG CCAGCACAGA GCTGACCACT 
TTAAAGAATA TTCGTGACTA CTTTAATCCA GCTACTCTGC CTCTAACACA GTACCTGATA 
GCAATGTCTT CATCAACTAC AATGAACAAC AAGAGAGTCA ATAAGAGAAA GTTTATCCCA 
CCAGCCTTCT CAAATATCAA CACAAAATTT GAACTACTCA GTATAGAAGC AACATTGAAG 
TTGGCTAGTG AGTTAATTCA GGCGCACAAG TTAAACAAAG ATCAGGCTGC AGCTCTAATT 
CAGATAGCGC AAATGATGGC ATCACGCCAG AGTGTTGAAG AAGCGAAGGA ACTGGCAACT 
CACACCCTCC CTATCACGAT CATCCACGGT GTTTTCGGAG CAGGGAAGAG TTACTTGCTG 
GCCGTGGTGA TTTTGTTTTT TGTGCAACTA TTTGAAAAAT GTGAAGCTCT TACAGTTGGA 
AATGCAAGAC CGTGGAAACT TCTAATTTCT TCTTCTACTA ATGTAGCTGT TGATAGAGTG 
CTTCTTGGGC TTCTTAGTCT TGGATTTGAA AAGTTTGTCA GAGTTGGGAG TGTCAGAAAG 
ATTGCCAAGC CAGTTTTACC TTACAGTTTG TATGCTGGCT CAGAAAATGA AAATGAACAA 
TTAAAAGAAC TACATGCACT CATGAAGGAA GACTTGACCC CTGTGGAAAG AGTCTATGTG 
AGGAAAAGTA TTGAGCAGCA TAAACTGGGG ACCAATAAAA CCTTGCTGAA ACAGGTTCGA 
GTAGTTGGAG TTACCTGTGC AGCCTGCCCG TTCCCATGCA TGAATGATTT GAAGTTCCCT 
GTGGTTGTCC TGGATGAGTG TAGTCAAATA ACAGAACCTG CTTCTCTCCT TCCCATTGCA 
AGGTTTGAGT GTGAAAAGCT GATACTTGTT GGAGATCCCC AACAGCTTCC TCCTACTATT 
CAGGGTTCTG ATGCAGCTCA CGAAAATGGA CTGGAACAAA CTCTTTTTGA CCGACTCTGC 
TTAATGGGTC ACAAGCCAAT TCTGTTGAGA ACTCAGTACC GTTGTCACCC CACAATCAGT 
GCTATTGCTA ATGATCTGTT TTATGAAGGA AATCTCATGA ATGGCATTTC CGAAACAGAG 
AGGAGCCCTT TACTGGAATG GCTTCCAACT CTGTGTTTCT ATAACGTAAA AGGACTGGAA 
CAGATTGAGA GAGGTAACAG CTTTCATAAT GTGGCAGAAG CTGCTTTTAC ACTCAAGTTG 
ATTCAATCAC TGATTGCAAG TGGAATAGCG GGCTCCATGA TTGGGGTGAT AACATTATAC 
AAATCCCAGA TGTACAAGCT CTGTCATCTA CTCAGTGCTG CGGACTTCGA CCATCCTAAC 
GTGAAAGCTG TGCAGGTGTC CACAGTAGAT GCTTTTCAGG GAGCTGAAAA GGAGATCATT 
ATTCTGTCCT GCGTAAGGAC CAGACAAGTA GGATTTATTG ATTCAGAAAA AAGAATGAAT 
GTTGCATTGA CCAGAGGAAG GAGGCATTTG TTGATTGTAG GAAATTTAGC CTGTCTGAGG 
AAAAATCGAC TATGGGGGCG TGTGATCCAA CACTGTGAAG GGAGGGAAGG TGGATTGCAG 
CATGCAAGCC AGTGTGAACC ACAACTGGAC CTTCTCCTTA AAGATTATCT TGAAAAACAA 
GCAGAAGAAA AACAGAAGAA AAAGGCTGAA AAAGATAAAT TGACAGATAA AAAATCTTAT 
TCACAAAAAG ACATGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179479.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034869.1 Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

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CloneID	OSm39513
Clone ID Related Accession (Same CDS sequence)	XM_012179479.2
Accession Version	XM_012179479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	protein ZGRF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG GAGGGAAGGT GGATTGCAGC 
ATGCAAGCCA GTGTGAACCA CAACTGGACC TTCTCCTTAA AGATTATCTT GAAAAACAAG 
CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034869.1
CDS	251..6592
Translation	MTTDYFLHLNLISEEIMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKTVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKINPEVLCEENYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKSTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLLMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILLREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGSEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATDQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPAPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKPSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDCDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCNLTAPDRVDEKVTDGFFAEAVPGTHFSNTGERENDAVNESSLDSVHLQMMKHMLLQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYTCDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDIKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVAHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTMNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPQQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCGGKVDCSMQASVNHNWTFSLKIILKNKQKKNRRKRLKKIN

Target ORF information:

RefSeq Version	XM_012179479.2
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179479.2

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAATAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA CGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA 
GAAGTGCTAT GTGAAGAAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA GCACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTTGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTCTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCAGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTGATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCGCGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGCAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ACCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACTGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCAAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGACGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC TACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAT 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATATAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGCCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAAT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGTGTT 
TTCGGAGCAG GGAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATTTGAA GTTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCCAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT TCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAGAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG GAGGGAAGGT GGATTGCAGC 
ATGCAAGCCA GTGTGAACCA CAACTGGACC TTCTCCTTAA AGATTATCTT GAAAAACAAG 
CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_012179473.3 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034863.2 Ovis aries zinc finger GRF-type containing 1 (ZGRF1), mRNA.

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CloneID	OSm81707
Clone ID Related Accession (Same CDS sequence)	XM_012179473.3
Accession Version	XM_012179473.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: protein ZGRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040257.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012179473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAGTAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA TGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTGACCCA 
GAAGTGCTAT GTGAANGAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA ACACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCTGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTAATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGTAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCCAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATCTGAA ATTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT CCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAAAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAAT ATGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034863.2
CDS	296..6823
Translation	MTTDYFLHLNLISEEVMESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGGCLESLFLKCPEVKPGDDLESDRYLITVEEVKVAGSTAVKQDVIKEAPALNSKRFMSSGRSLGCQPAGLKRKFTGFQGPRQVPKKMVITENSEPAASLESEKPGPVFLSPLYSTPPLFSTIGKKDTSSMPTDSENIVTYKNRERNGIPFSSVISTPSFKIDPEVLCEnullNYVCSPVSSVNKHSDSLLTDDESMKRDRLASHALGVSQNIRSKAQILALLKSTSTSTSKELNSEIIGHFPQIQPQGSLEIPAEPNCLIEQEQYAEVKNTESLHNQHQSENAMRNKSRWAMYLSSQSSPAHSSTVEGKDTEEKPKTQEGNLNLKDLSMQKRIQFFETCAEKGKRYNEDKPIDDNDQSWDQEGKLEIPSLYENSSLPINCSIVVNDGLLLDSDIQKNNKRSVDQNDQIGKKGSILVREKAQETNINGTPEKKYDHLQIESSLSNNSGISDDIFSKSTNDENLSSIHEPMSDISQPLVEVTFNLNNFETSDTEEESQESSKISQDSEYWVKATLVNSSNSRCENISCKEVGCEHLPLLTSTGGKLSETFPMKEILPSQFCDRTCVGYDTGTWKAGNTGKERKEVCSDTLSNFDSSLEWTDDRYVENDEGANKSISASFPSKSESMNANLHIPHFLNIATNQKPENNLFSEQAQTRPFIVGSDLDKNTNQVLPLTSSSGNSTQLLNAKQIHSKECIALDKSQTQASNSLCYPLGKTHISKDTESHIPESEEVGRSQSLSQDHIDVETSGESKQYWNNIRNSSEISGLVNSISLLKSLTEHSTALEGLEALKNTSFKQETLQTYEPDSNPEAGKPLTTIVSQAIPKSPHLNQDPHQMIKENEAEPSESLQSLQFFPLGTEEKTAFLAVIPKQVEREAGKDPTPVEFQGHQVKGSASSTVVVRGHSSQVECNQVLDGTVYESRTTNACSLTPALPSTCMQTDFLQFPNEENFPKGETSFTIQDNFQVRAEFNKDLTKNDSILDFPPKDSDHLYNYLDNNFKSTERTSWEKNKVMSPEQKISTLSPVSTLSFNSRDDDFMLEFSEESLKTQTLPVMKKSSSLENKKNLQRLSFEVSRTRAPLVTLPPASGPPDLDSCSFMMDRDRQKSSGSPIFSLCEESAVPSFSFGPKDQSEASFSNESREVTPSDVSLLDNTSTQSKWLKYQNIRQCHLTAPDRVDEKVTDGFFAEAVPGMHFSNTGERENDAVNESSLDSVHLQMMKHMLQQQQQDFSSQDSVCRKKAFSLNVNEASKTEEIQNLLGEYAYYNCSLTGGLQEVNDSELCFPSGQKVKSACLPQRQIHIPAVFQSPAHYKQIFTSCLTEHLNILLFGLAQRLHKALSKVDISFYTSSDGDKLKNVDNNVPSCHHQQPAKLVMVKKEGPNKGRLFYACDGPKANQCKFFKWFEEMTPGYSTQEKSLPSMVLSDTKSIGFYLRSQNIPLYEECQLFVRKGFDFQRKQYGKLKKFTTVDPEFYNEPKSKLYLKLSRKESSSAYSKDDLWVVSKTLDFELDTFIACSTFFGPSSINEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIRDYFNPATLPLTQYLIAMSSSTTVNNKRVNKRKFIPPAFSNINTKFELLSIEATLKLASELIQAHKLNKDQAAALIQIAQMMASRQSVEEAKELATHTLPITIIHGVFGAGKSYLLAVVILFFVQLFEKCEALTVGNARPWKLLISSSTNVAVDRVLLGLLSLGFEKFVRVGSVRKIAKPVLPYSLYAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPTISAIANDLFYEGNLMNGISETERSPLLEWLPTLCFYNVKGLEQIERGNSFHNVAEAAFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAADFDHPNVKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLACLRKNRLWGRVIQHCEGREGGLQHASQYEPQLDLLLKDYLEKQAEEKQKKKAEKDKLTDKKSYSQKDMV

Target ORF information:

RefSeq Version	XM_012179473.3
Organism	Ovis aries(sheep)
Definition	Ovis aries zinc finger GRF-type containing 1 (ZGRF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179473.3

ORF Insert Sequence:

ATGACTACTG ATTATTTTCT GCACCTGAAC CTAATTTCAG AAGAAGTAAT GGAAAGCCAA 
GAATTTATTG TACTATATAC TCATCAAAAG ATGAAGAAGT CAAAAGTGTG GCAAGATGGA 
ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT 
TTGGAGAGTT TATTTCTTAA GTGCCCTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT 
CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT 
GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT 
GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTTACTGGTT TTCAAGGACC ACGTCAAGTG 
CCAAAGAAAA TGGTTATTAC AGAAAATAGT GAACCAGCTG CTTCACTTGA GTCTGAGAAA 
CCTGGTCCTG TTTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTTC TACAATTGGC 
AAGAAAGATA CAAGTAGTAT GCCAACAGAC TCTGAGAACA TTGTCACTTA TAAGAACAGA 
GAGCGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTGACCCA 
GAAGTGCTAT GTGAANGAAA TTATGTTTGC TCTCCTGTCA GTTCTGTAAA TAAGCATTCA 
GATTCTTTAC TGACCGATGA TGAGTCCATG AAAAGAGATC GTTTGGCATC TCACGCTTTA 
GGAGTTTCAC AAAACATTAG AAGCAAAGCC CAGATATTGG CTCTTCTGAA GTCCACATCC 
ACAAGTACGT CTAAGGAGCT AAATTCTGAA ATTATAGGCC ATTTCCCTCA GATACAGCCA 
CAAGGAAGTT TAGAAATTCC TGCTGAACCA AATTGCTTAA TTGAACAGGA ACAGTATGCT 
GAAGTGAAGA ACACAGAAAG TTTGCACAAC CAGCATCAAT CAGAAAACGC CATGAGAAAT 
AAAAGCCGGT GGGCCATGTA TTTATCCTCA CAGAGTTCAC CTGCACATTC TTCTACAGTA 
GAAGGAAAAG ATACGGAAGA GAAACCCAAG ACCCAAGAAG GTAATTTAAA CTTGAAAGAC 
CTTTCGATGC AAAAAAGGAT CCAGTTCTTT GAAACATGTG CTGAAAAGGG AAAAAGGTAT 
AATGAAGATA AGCCAATAGA TGATAATGAT CAATCCTGGG ATCAAGAAGG AAAACTAGAA 
ATTCCTTCAC TCTATGAAAA CAGTAGCTTA CCAATTAATT GTAGCATTGT AGTAAATGAT 
GGCCTATTGT TAGATTCTGA CATTCAAAAA AATAATAAAA GATCTGTTGA TCAAAATGAC 
CAGATAGGCA AAAAAGGATC AATTCTTGTT AGAGAAAAAG CACAGGAGAC AAATATAAAT 
GGAACACCAG AAAAGAAGTA TGATCATCTA CAAATTGAAT CTTCACTAAG TAACAATTCT 
GGGATCTCTG ATGATATATT TTCTAAAAGC ACTAACGATG AAAACCTTAG TAGTATTCAT 
GAACCCATGA GTGATATATC ACAGCCACTT GTGGAAGTCA CATTTAATCT AAACAATTTC 
GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAATTTCCCA GGATTCAGAG 
TATTGGGTGA AGGCAACTTT GGTTAATAGC AGCAATTCAA GATGTGAGAA TATAAGTTGT 
AAAGAAGTTG GCTGTGAACA TCTGCCTCTC TTAACGTCTA CTGGGGGCAA ACTTTCAGAG 
ACATTTCCCA TGAAAGAGAT TCTGCCTTCA CAGTTTTGTG ATAGAACTTG TGTAGGTTAT 
GACACAGGAA CTTGGAAAGC TGGAAACACT GGGAAAGAAA GAAAGGAGGT GTGTAGTGAC 
ACATTAAGCA ATTTTGACTC ATCTTTAGAG TGGACTGATG ATAGATATGT AGAAAATGAT 
GAAGGCGCTA ATAAATCCAT TAGTGCTTCC TTCCCAAGTA AATCTGAAAG TATGAATGCA 
AATTTACATA TTCCTCATTT TTTAAACATA GCCACTAATC AGAAACCTGA AAACAACCTA 
TTTTCAGAAC AGGCTCAGAC TCGGCCTTTT ATTGTGGGAA GTGACTTAGA CAAAAATACC 
AATCAGGTTT TACCATTAAC CTCTAGCAGT GGTAACAGTA CCCAGCTATT AAATGCCAAA 
CAGATTCACT CTAAAGAATG TATTGCACTT GATAAATCAC AGACCCAAGC TTCCAATTCT 
TTGTGTTACC CTTTGGGAAA AACACATATT TCCAAAGACA CAGAATCACA TATTCCTGAA 
TCTGAAGAGG TGGGAAGGAG TCAAAGTTTA TCCCAGGACC ATATTGATGT AGAAACTTCT 
GGAGAAAGCA AACAATACTG GAATAATATT AGAAATTCTT CAGAAATTTC TGGATTAGTA 
AATAGCATTT CTCTCTTAAA ATCATTGACT GAACACAGTA CGGCTTTGGA AGGCTTGGAA 
GCATTGAAAA ATACCTCCTT TAAACAAGAA ACTCTGCAGA CATATGAGCC AGATAGCAAT 
CCTGAAGCTG GGAAACCATT GACTACAATT GTTTCACAAG CTATACCAAA GTCTCCTCAT 
CTGAACCAGG ATCCTCATCA GATGATAAAA GAAAATGAAG CTGAACCAAG TGAATCACTT 
CAGTCTTTGC AGTTCTTTCC CTTGGGCACT GAAGAAAAGA CTGCTTTTCT AGCTGTTATT 
CCTAAACAAG TGGAGAGAGA AGCCGGTAAA GACCCCACGC CTGTTGAGTT TCAAGGGCAC 
CAAGTGAAAG GATCTGCTTC TAGCACTGTG GTGGTCAGAG GACACAGCTC GCAGGTCGAA 
TGCAATCAGG TTTTGGATGG CACTGTGTAT GAAAGCCGCA CAACAAACGC TTGTTCTTTG 
ACACCAGCGT TGCCTAGTAC TTGTATGCAG ACTGACTTCT TGCAGTTTCC AAATGAAGAA 
AACTTTCCAA AAGGAGAAAC TAGCTTTACT ATTCAAGACA ATTTTCAAGT GAGAGCTGAG 
TTTAATAAGG ACTTGACTAA AAATGATTCA ATTTTAGATT TCCCTCCTAA AGATTCAGAC 
CATTTATATA ATTATTTGGA TAATAATTTT AAATCAACAG AGAGGACTTC GTGGGAAAAA 
AATAAGGTGA TGTCACCAGA ACAGAAGATC TCAACATTAA GCCCTGTTTC TACTTTATCT 
TTTAACTCAA GAGATGATGA CTTCATGCTA GAATTCTCTG AGGAGTCCCT GAAAACGCAA 
ACTTTACCTG TAATGAAAAA ATCAAGTTCT CTGGAGAATA AGAAGAACCT TCAAAGGCTT 
TCCTTTGAAG TAAGTAGAAC CCGAGCTCCA CTTGTTACTT TGCCACCTGC CAGTGGGCCA 
CCTGACTTAG ATTCTTGTTC ATTTATGATG GACCGTGACA GGCAAAAGTC TTCCGGTTCT 
CCCATATTCA GCCTGTGTGA AGAGTCAGCA GTTCCTTCTT TTAGCTTTGG GCCCAAGGAC 
CAAAGTGAAG CTTCTTTCTC TAACGAATCT AGGGAAGTGA CTCCAAGTGA TGTTTCACTT 
CTTGATAATA CATCTACTCA AAGCAAGTGG CTGAAATATC AAAATATACG CCAGTGCCAC 
TTGACTGCTC CAGACAGAGT TGATGAGAAA GTAACAGATG GCTTCTTTGC TGAGGCTGTT 
CCTGGGATGC ATTTTAGTAA CACAGGTGAA AGAGAGAATG ATGCTGTGAA TGAAAGTTCT 
TTAGACTCTG TGCATTTACA AATGATGAAA CACATGCTTC AACAGCAGCA GCAGGATTTT 
AGCAGTCAAG ATTCGGTTTG CAGAAAGAAG GCATTTTCTT TGAATGTAAA CGAGGCTTCT 
AAGACCGAGG AAATTCAGAA TTTGTTAGGA GAGTATGCCT ACTACAACTG CAGTCTAACA 
GGAGGTTTAC AGGAGGTAAA TGACTCTGAG CTATGCTTCC CAAGTGGACA GAAAGTAAAA 
TCTGCATGTC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA TTTTTACATC TTGTCTCACA GAACATTTAA ATATATTGCT ATTTGGATTG 
GCACAAAGGT TGCACAAAGC TCTTTCAAAA GTTGACATAT CATTTTACAC ATCATCAGAT 
GGAGACAAAC TGAAAAATGT AGACAATAAT GTGCCATCCT GCCATCATCA GCAGCCTGCA 
AAACTGGTCA TGGTGAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA CGCCTGTGAT 
GGACCCAAAG CTAACCAATG TAAGTTTTTT AAGTGGTTTG AAGAAATGAC CCCAGGATAC 
TCAACACAGG AAAAATCTCT ACCTAGCATG GTTTTAAGTG ATACAAAAAG TATTGGCTTC 
TACTTAAGAA GTCAAAACAT ACCCCTCTAT GAGGAATGCC AACTTTTCGT GAGAAAAGGA 
TTTGATTTTC AAAGAAAACA GTACGGTAAA CTAAAGAAGT TTACTACTGT AGATCCTGAA 
TTTTATAATG AACCTAAGAG CAAACTTTAT CTTAAACTGA GTCGGAAAGA AAGTTCATCT 
GCTTACAGCA AAGATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA ATTGGATACT 
TTTATTGCAT GTAGTACTTT CTTTGGACCA TCATCTATCA ATGAGGTAGA GCTTCTACCT 
TTGAAAGGTT ATTTCCCTTC AAACTGGCCC ACTAACACAG TGGTCCACGC CTTATTGGTT 
TGTAATGCCA GCACAGAGCT GACCACTTTA AAGAATATTC GTGACTACTT TAATCCAGCT 
ACTCTGCCTC TAACACAGTA CCTGATAGCA ATGTCTTCAT CAACTACAGT GAACAACAAG 
AGAGTCAATA AGAGAAAGTT TATCCCACCA GCCTTCTCAA ATATCAACAC AAAATTTGAA 
CTACTCAGTA TAGAAGCAAC ATTGAAGTTG GCTAGTGAGT TAATTCAGGC GCACAAGTTA 
AACAAAGATC AGGCTGCAGC TCTAATTCAG ATAGCGCAAA TGATGGCATC ACGCCAGAGT 
GTTGAAGAAG CGAAGGAACT GGCAACTCAC ACCCTCCCTA TCACGATCAT CCACGGCGTT 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCC GTGGTGATTT TGTTTTTTGT GCAACTATTT 
GAAAAATGTG AAGCTCTTAC AGTTGGAAAT GCAAGACCGT GGAAACTTCT AATTTCTTCT 
TCTACTAATG TAGCTGTTGA TAGAGTGCTT CTTGGGCTTC TTAGTCTTGG ATTTGAAAAG 
TTTGTCAGAG TTGGGAGTGT CAGAAAGATT GCCAAGCCAG TTTTACCTTA CAGTTTGTAT 
GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTAC ATGCACTCAT GAAGGAAGAC 
TTGACCCCTG TGGAAAGAGT CTATGTGAGG AAAAGTATTG AGCAGCATAA ACTGGGGACC 
AATAAAACCT TGCTGAAACA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCGTTC 
CCATGCATGA ATGATCTGAA ATTCCCTGTG GTTGTCCTGG ATGAGTGTAG TCAAATAACA 
GAACCTGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT ACTTGTTGGA 
GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCACGA AAATGGACTG 
GAACAAACTC TTTTTGACCG ACTCTGCTTA ATGGGTCACA AGCCAATTCT GTTGAGAACT 
CAGTACCGTT GTCACCCCAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAT 
CTCATGAATG GCATTTCCGA AACAGAGAGG AGCCCTTTAC TGGAATGGCT CCCAACTCTG 
TGTTTCTATA ACGTAAAAGG ACTGGAACAG ATTGAAAGAG GTAACAGCTT TCATAATGTG 
GCAGAAGCTG CTTTTACACT CAAGTTGATT CAATCACTGA TTGCAAGTGG AATAGCGGGC 
TCCATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTCTG TCATCTACTC 
AGTGCTGCGG ACTTCGACCA TCCTAACGTG AAAGCTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGCG TAAGGACCAG ACAAGTAGGA 
TTTATTGATT CAGAAAAAAG AATGAATGTT GCATTGACCA GAGGAAGGAG GCATTTGTTG 
ATTGTAGGAA ATTTAGCCTG TCTGAGGAAA AATCGACTAT GGGGGCGTGT GATCCAACAC 
TGTGAAGGGA GGGAAGGTGG ATTGCAGCAT GCAAGCCAAT ATGAACCACA ACTGGACCTT 
CTCCTTAAAG ATTATCTTGA AAAACAAGCA GAAGAAAAAC AGAAGAAAAA GGCTGAAAAA 
GATAAATTGA CAGATAAAAA ATCTTATTCA CAAAAAGACA TGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ZGRF1
Entrez Gene ID	101118531
Full Name	zinc finger GRF-type containing 1
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ZGRF1 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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