nhsl1 gene cDNA ORF clone, Oryzias latipes(Japanese medaka)

mRNA	Protein	Name
XM_023953325.1	XP_023809093.1	NHS-like protein 1 isoform X7
XM_020714401.1	XP_020570060.1	NHS-like protein 1 isoform X1
XM_020714404.1	XP_020570063.1	NHS-like protein 1 isoform X3
XM_020714405.1	XP_020570064.1	NHS-like protein 1 isoform X4
XM_023953319.1	XP_023809087.1	NHS-like protein 1 isoform X1
XM_023953320.1	XP_023809088.1	NHS-like protein 1 isoform X2
XM_023953322.1	XP_023809090.1	NHS-like protein 1 isoform X4
XM_023953323.1	XP_023809091.1	NHS-like protein 1 isoform X5
XM_023953321.1	XP_023809089.1	NHS-like protein 1 isoform X3
XM_023953326.1	XP_023809094.1	NHS-like protein 1 isoform X8
XM_020714406.1	XP_020570065.1	NHS-like protein 1 isoform X5
XM_020714407.1	XP_020570066.1	NHS-like protein 1 isoform X6
XM_020714408.1	XP_020570067.1	NHS-like protein 1 isoform X7
XM_020714402.1	XP_020570061.1	NHS-like protein 1 isoform X1
XM_020714403.1	XP_020570062.1	NHS-like protein 1 isoform X2
XM_020714409.1	XP_020570068.1	NHS-like protein 1 isoform X8
XM_023953324.1	XP_023809092.1	NHS-like protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa114918	XM_023953319.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114919	XM_023953320.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114920	XM_023953321.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114921	XM_023953322.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114922	XM_023953323.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114923	XM_023953324.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114924	XM_023953325.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa114925	XM_023953326.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76379	XM_020714402.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76379	XM_020714401.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76380	XM_020714403.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76381	XM_020714404.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76382	XM_020714405.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76383	XM_020714406.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76384	XM_020714407.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76385	XM_020714408.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa76386	XM_020714409.1 Latest version!	Oryzias latipes NHS like 1 (nhsl1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

nhsl1 ( XM_023953319.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809087.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa114918
Clone ID Related Accession (Same CDS sequence)	XM_023953319.1
Accession Version	XM_023953319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GAAGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809087.1
CDS	329..5152
Translation	MPFHQRTVEPRRVSRLSARYGWKAREEEPGKRKTRRLVLFSSLDEVSCHTLTGLIHQLSDLSRHASDIFLGIEVEAGAVFRRSCRIQGRLQFLQDEVCRLDPKKVKIPVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953319.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953319.1

ORF Insert Sequence:

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GAAGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953320.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809088.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa114919
Clone ID Related Accession (Same CDS sequence)	XM_023953320.1
Accession Version	XM_023953320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACAT ACATGCACTG CCTCTCCTCG GAGCCGGCGG TCAGTTGCTG GCACGGCGCC 
TGCTATGAGG ACCCCTCTGG CCAGAACAAA ACTCTTTGCT CCTTCAAGCC CAGATGCGAG 
ATGGGGAACA AACTCCAGGT TCAGGCCCTC CCCAGTTCCA ACCCGTGCAG ACCGGAGGGG 
GGCCTGCGCA GGCGGCTGCT CTCTGTGTCG AAAGTGCACC AGAAGCCGGA CTCGCTCTGG 
ACTCCCAAAC CTCTGCTGGG ACCCGCGGCC GATGACTTAC AGAGGAAAAC ACAAAGTTGT 
CAGGATGGGA AAGCTGTGTC AAACCTGGAT GAGGAGAGCA AATGGACGGT ACACTACACG 
GCTCCTTGGC ACCAGCAGGA AAACGTCTTC CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG 
GACCTCCACC GCCAAGCCAA AGTCAACCTC AAGACCACCC TGCGAGAATG TGATAAGTTG 
AGGAAAGATG GTTTCCGGAG CTCCCAGTAC TACTCTCAGG GCCCGACCTT CTCCGAGCCC 
TTACAGTCCA GCAGCAGCCT GCAAGACGAG GAAGATGAGG AAAATGACAA GAAGTCTTTA 
GCATCCTCGA TGGAGGATGA TAAATCCCAG CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT 
GAAAGGGGCG AGATGTTGGA AGTTGACGGG CAGATGGTGT GGAATAAAGC GGTTCCCCTC 
CCGACGCCGG AGGAGAGGAT GAGGCAAGCA GCTCAGGCCG TGCCCACGGA CATAGTCCCC 
ATCAATGTCA CAGGGGCAGT GTTTGACCGC CAGGCGAGCA TCCGGCGCTC CCTCATTAAC 
ACTGATACCG TGTCCCGTCG GCCCAAGAAG GTCAAACGCA GAAAGACTAT ATCAGGGCTG 
CCTGACAACT TCAACCAGGA GCTAGCTAAA GGACGAGGCG GGGAACTTCG GCCACATTCC 
ATGTTCATCC CAGGACAGTA CTCCACTTTG GGGAGAGTTG GGAGTGTCAA CTCAACACTC 
CGCTTCGAGA AAAGAGACTC CAGCTGCCAG ACAGAAGAAG TGAAGATTGT CCCTCCATCT 
GTGAGAAGAA TTCGAGCACA AAGAGGGCAG GGAATCGCCG CTCAAATGGC TGGGATATCT 
GCTTCCTCTT CTACTGGAAG CATCTCCATC TCGAGCACAG ATGGCTTGAT TCTGCCCAAT 
CAGTTTTTCA GAGACCCTTC CCGCTTTCAC AGTCTGCCGC GACAGGGTGC CAGAGTGTCC 
CTCAGTGCTG ATCCCATCTA TAGCAGCACG CCCATTAAGT CAGAGGATCA GTTGCAGAGG 
AATATTGGAA AATTCCAGGT TGACGAAACA GCGGTACACA TCAGGAATGC CCCACGAACG 
AGCACCTTGC CCAGACCCAC GTCTCAGGAG GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA 
GGTGGTCCAG CATGTGTGGT CTCCCCTCAG GCAGCTTATT CCACCTCCTT CATTCCCAAT 
GCCACCCTGT CCAACTCCAC GGATGTTATC ACCCTCAGCT CATCTAGTCA TTTATCCTTA 
TCAGCTGTCT CAGCTTTGGC CACTTCTACC CCCACGCGTG CACCACAAGA GAACGGTGTG 
ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC CACTTGGATG GAAGTGTTCA TAGTCACAGT 
ACTTTGGCTC CCACTTCACC ATCATGTCCT CCAGAAGAAC AGTGGATCTA TGACTCACCC 
GAAAATGTGG TTCCACACCG CACTCTGACC TCCAGCTGTT CCACTCCCAT CAACCAGCTT 
TATAGCAGCC TGGACCATTC CTCACGGACT GATTCCAGCT CCCTCTACTC CCAGGACAAC 
GATGGATACT TCACCTCTAT GCACATGGAC TCAGGGCTGC GCTCCCGAAG TCACGGAAGT 
GGGCACGGCG CAGGAGCTGG GCGGTCCCGA CACAGCATGT ATGAGTGTCG CGAGATGGCC 
AATCGAGAAG ACTCTGGAAG CGTGTACAGC GACCGCTCTC TGTCGCGCAG CATCTCGCTC 
CGCAAGTCAA AGAAGCCACC GCCACCTCCG GCACGCACAG ATTCTCTGAG GCGCAAGCCT 
GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT CCAACAACTG TTGCCAATGG GGACGGTGCT 
AACATGCTCA ATGAAACGTT CCTTGCTAGC TTGCAGCAGA GCCTGCAAAT GGGACTGAGG 
GGGTCAAAGG GAAAGGGTAC CTCTCTATCT TCACCCTCTC ACAGCCCCAG CAGTGACTAT 
GATGACCCTT GGTTGTTGCG ACCGCGCAGT CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC 
GCCTCATTAG CTAACACAAA TGGTGTGTCT AATGTCTACT CATTATGCCA TGTCACGCCC 
ACGCATAGTG ACACCAGCAG TTTGCGTTCT GACTATGCAG AGTCCTGGGG ATACTACATG 
GACTACCCTC GTAACCCTGG AGACCAGGGG GGGCAGACCC CACCAGCCCA TGCTACAGAT 
AACATGTCAG CTGGGGGTCA TCCAGGAGTC CTACAGAATG GAGGAGAGAT TCACAGCAAT 
ACTCAAACAG ATGGAGCTCC AGGCCAGGAG GGAGGGCAGT CAGGGAAGCC CAAACCATCC 
AGCTCCTCAC CGGACAGGGT GCACAGACTT ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG 
TCCAACACGC CTACAGCCGG AACCCCGGTG CCAGCCTTCG TCAGATCCAT GTCTCCCTCC 
TCAGGGAGTC GGCCCAAACC CAAAGTGCCG GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT 
TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC TCCAACACTT CAGACTCACT AAAGAGTTCA 
GGTCCTCCTC TGCCACCCCC TAATCCCCTC CATCTCACCT CTTCTACCCC AAACACCCCT 
CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT CTTCCTCCAA ATTCTACATC AGCTCCCTCA 
TCTCCTCCTC CACCTCCTCC ATTACCCAAC TCTCCAAGAG AAACCTTCTT GGCCCCTCTA 
TCTGCGTCAC CAGAATTCCC TCCTCCTCCA TCTCCAGACC TGTTGATGCA CCACAGTTCG 
TCCTTAAATG GGAGCTTTAG TCCTCCACCT CCCCCTGAGA CCGGGATCCC TCCACCACCT 
CCACTGCCTG ACTTCACCCC AGCTTTTGGA TCCCCAAATA TTAGGAAATT GACAAAAAAT 
ACTCCTAAAC CACATCCTTC CAAACACTCC ACAGAGTCCC CCAAGCCCCT CATCACCCCC 
TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC GTCAAGCGAT CACAAGAGGA GATCACTGGG 
GAGCTGGACC AAATGAAACC TCAGGAAAAT GGACTGGACC TTCTCCAGGA GCTGAAACTC 
CATAGTCTAG ATCCATCCCC CATCCAGGAG CACACCACTG TCACGAGTAG CTCTCCAGAC 
GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT GTGACGAAAC TGCTGGAAGA GCTGAGTCTG 
GACTGCAGTT TTATAATCGA CACACAGGAT AGTGGTGTCA TCAATGGAAA AGCTGAGGAC 
CAGAGTTACT GTCTATCAAA TGGAAAAGAC AATGAGGAAG AGCTGCCACT GCCTAGCTGT 
CCAGAGAGAT GTGGAAGCTC CTCCATCAAA GCCAAGCCTC CGCCAGTCTC CAAGAAACCC 
AAACTTACCT TTGTTCCATC CCAACCAATC AGCGAGCCAG CTCAAACTCA AAATGACAAT 
GCAGCCAGTC TGTCGCCAGA GGATCAGGTC GATGCACAAA CGACAAATGA CGTAACAAAT 
AAGAGGAACA ACCGGGAGGA AGAGGCGTCA GAGAGCACAA AGGCTTCCAC TGAGAACCTG 
GACCAGAGCT GCCAAGATGC AGATCTTGAC CACGACATTT GCACTAATGG AGAGGTGTTT 
GATGAGGATG AAGAAGAGGA AGAAGGAGAA GGAGCAAGCA GCACCACGGG GTCCATCAGC 
TCAAAAGAGG AAGACACAAG TGAAGTTTTC GAATCCAGAC CGGCTGAACC TCCTTCACCC 
CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA ATGGTGACCC CGACTCCCTC CAAACCCCGA 
ACCACTGATG ACCTTTTTGC TGTCATACAC AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA 
GAGTCGGATG AAGACAAGTC CCGCCCCGGG GTTCAGGCAC AGTCTCCACC CTCCACCCCG 
TCACCCGTCT CTCCTGGGCC GGTTTCTCCG TCGGCCCGTC AGATGGGTTC CATCCAGCGA 
AACCTACGCA AGTCCGCCAC CAGCAGCGAC AACTTCAAGG CCCTCCTGCT TCGGAAGGGA 
AGCCGCTCTG AGAACAGTTT CCGCATGTCT GCTACTGAAA TGCTGCGCTC CACCGACCCG 
CGCTTCCAGC GAACTCGCTC CGAGTCTGCG CTGGACCCCC CCACTGCGTC TCCCACGTCA 
CCGATGGCGC CTCACAGCCC CTCTGCCTCT TTTGGGCGGA GTAAGAGGGT AACGGATGAG 
TGGAGCCGCT ACGATGCCCT GTCCTCGCCG ACATATTCGC CTGGATCAAA GTTCGGGCGC 
TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC AAATACAACA CTCGCAGCCG AATCCTCAGC 
AGCCCGATGA CTGTGATCTG TGAGCGTGAA GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG 
GTCGGCGAGA GCCGGGAAGG AGTCCAGACG CTTCCAGAAA CCAACGGCAC TTTATCCGAA 
GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809088.1
CDS	50..4864
Translation	MITYMHCLSSEPAVSCWHGACYEDPSGQNKTLCSFKPRCEMGNKLQVQALPSSNPCRPEGGLRRRLLSVSKVHQKPDSLWTPKPLLGPAADDLQRKTQSCQDGKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953320.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953320.1

ORF Insert Sequence:

ATGATCACAT ACATGCACTG CCTCTCCTCG GAGCCGGCGG TCAGTTGCTG GCACGGCGCC 
TGCTATGAGG ACCCCTCTGG CCAGAACAAA ACTCTTTGCT CCTTCAAGCC CAGATGCGAG 
ATGGGGAACA AACTCCAGGT TCAGGCCCTC CCCAGTTCCA ACCCGTGCAG ACCGGAGGGG 
GGCCTGCGCA GGCGGCTGCT CTCTGTGTCG AAAGTGCACC AGAAGCCGGA CTCGCTCTGG 
ACTCCCAAAC CTCTGCTGGG ACCCGCGGCC GATGACTTAC AGAGGAAAAC ACAAAGTTGT 
CAGGATGGGA AAGCTGTGTC AAACCTGGAT GAGGAGAGCA AATGGACGGT ACACTACACG 
GCTCCTTGGC ACCAGCAGGA AAACGTCTTC CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG 
GACCTCCACC GCCAAGCCAA AGTCAACCTC AAGACCACCC TGCGAGAATG TGATAAGTTG 
AGGAAAGATG GTTTCCGGAG CTCCCAGTAC TACTCTCAGG GCCCGACCTT CTCCGAGCCC 
TTACAGTCCA GCAGCAGCCT GCAAGACGAG GAAGATGAGG AAAATGACAA GAAGTCTTTA 
GCATCCTCGA TGGAGGATGA TAAATCCCAG CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT 
GAAAGGGGCG AGATGTTGGA AGTTGACGGG CAGATGGTGT GGAATAAAGC GGTTCCCCTC 
CCGACGCCGG AGGAGAGGAT GAGGCAAGCA GCTCAGGCCG TGCCCACGGA CATAGTCCCC 
ATCAATGTCA CAGGGGCAGT GTTTGACCGC CAGGCGAGCA TCCGGCGCTC CCTCATTAAC 
ACTGATACCG TGTCCCGTCG GCCCAAGAAG GTCAAACGCA GAAAGACTAT ATCAGGGCTG 
CCTGACAACT TCAACCAGGA GCTAGCTAAA GGACGAGGCG GGGAACTTCG GCCACATTCC 
ATGTTCATCC CAGGACAGTA CTCCACTTTG GGGAGAGTTG GGAGTGTCAA CTCAACACTC 
CGCTTCGAGA AAAGAGACTC CAGCTGCCAG ACAGAAGAAG TGAAGATTGT CCCTCCATCT 
GTGAGAAGAA TTCGAGCACA AAGAGGGCAG GGAATCGCCG CTCAAATGGC TGGGATATCT 
GCTTCCTCTT CTACTGGAAG CATCTCCATC TCGAGCACAG ATGGCTTGAT TCTGCCCAAT 
CAGTTTTTCA GAGACCCTTC CCGCTTTCAC AGTCTGCCGC GACAGGGTGC CAGAGTGTCC 
CTCAGTGCTG ATCCCATCTA TAGCAGCACG CCCATTAAGT CAGAGGATCA GTTGCAGAGG 
AATATTGGAA AATTCCAGGT TGACGAAACA GCGGTACACA TCAGGAATGC CCCACGAACG 
AGCACCTTGC CCAGACCCAC GTCTCAGGAG GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA 
GGTGGTCCAG CATGTGTGGT CTCCCCTCAG GCAGCTTATT CCACCTCCTT CATTCCCAAT 
GCCACCCTGT CCAACTCCAC GGATGTTATC ACCCTCAGCT CATCTAGTCA TTTATCCTTA 
TCAGCTGTCT CAGCTTTGGC CACTTCTACC CCCACGCGTG CACCACAAGA GAACGGTGTG 
ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC CACTTGGATG GAAGTGTTCA TAGTCACAGT 
ACTTTGGCTC CCACTTCACC ATCATGTCCT CCAGAAGAAC AGTGGATCTA TGACTCACCC 
GAAAATGTGG TTCCACACCG CACTCTGACC TCCAGCTGTT CCACTCCCAT CAACCAGCTT 
TATAGCAGCC TGGACCATTC CTCACGGACT GATTCCAGCT CCCTCTACTC CCAGGACAAC 
GATGGATACT TCACCTCTAT GCACATGGAC TCAGGGCTGC GCTCCCGAAG TCACGGAAGT 
GGGCACGGCG CAGGAGCTGG GCGGTCCCGA CACAGCATGT ATGAGTGTCG CGAGATGGCC 
AATCGAGAAG ACTCTGGAAG CGTGTACAGC GACCGCTCTC TGTCGCGCAG CATCTCGCTC 
CGCAAGTCAA AGAAGCCACC GCCACCTCCG GCACGCACAG ATTCTCTGAG GCGCAAGCCT 
GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT CCAACAACTG TTGCCAATGG GGACGGTGCT 
AACATGCTCA ATGAAACGTT CCTTGCTAGC TTGCAGCAGA GCCTGCAAAT GGGACTGAGG 
GGGTCAAAGG GAAAGGGTAC CTCTCTATCT TCACCCTCTC ACAGCCCCAG CAGTGACTAT 
GATGACCCTT GGTTGTTGCG ACCGCGCAGT CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC 
GCCTCATTAG CTAACACAAA TGGTGTGTCT AATGTCTACT CATTATGCCA TGTCACGCCC 
ACGCATAGTG ACACCAGCAG TTTGCGTTCT GACTATGCAG AGTCCTGGGG ATACTACATG 
GACTACCCTC GTAACCCTGG AGACCAGGGG GGGCAGACCC CACCAGCCCA TGCTACAGAT 
AACATGTCAG CTGGGGGTCA TCCAGGAGTC CTACAGAATG GAGGAGAGAT TCACAGCAAT 
ACTCAAACAG ATGGAGCTCC AGGCCAGGAG GGAGGGCAGT CAGGGAAGCC CAAACCATCC 
AGCTCCTCAC CGGACAGGGT GCACAGACTT ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG 
TCCAACACGC CTACAGCCGG AACCCCGGTG CCAGCCTTCG TCAGATCCAT GTCTCCCTCC 
TCAGGGAGTC GGCCCAAACC CAAAGTGCCG GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT 
TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC TCCAACACTT CAGACTCACT AAAGAGTTCA 
GGTCCTCCTC TGCCACCCCC TAATCCCCTC CATCTCACCT CTTCTACCCC AAACACCCCT 
CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT CTTCCTCCAA ATTCTACATC AGCTCCCTCA 
TCTCCTCCTC CACCTCCTCC ATTACCCAAC TCTCCAAGAG AAACCTTCTT GGCCCCTCTA 
TCTGCGTCAC CAGAATTCCC TCCTCCTCCA TCTCCAGACC TGTTGATGCA CCACAGTTCG 
TCCTTAAATG GGAGCTTTAG TCCTCCACCT CCCCCTGAGA CCGGGATCCC TCCACCACCT 
CCACTGCCTG ACTTCACCCC AGCTTTTGGA TCCCCAAATA TTAGGAAATT GACAAAAAAT 
ACTCCTAAAC CACATCCTTC CAAACACTCC ACAGAGTCCC CCAAGCCCCT CATCACCCCC 
TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC GTCAAGCGAT CACAAGAGGA GATCACTGGG 
GAGCTGGACC AAATGAAACC TCAGGAAAAT GGACTGGACC TTCTCCAGGA GCTGAAACTC 
CATAGTCTAG ATCCATCCCC CATCCAGGAG CACACCACTG TCACGAGTAG CTCTCCAGAC 
GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT GTGACGAAAC TGCTGGAAGA GCTGAGTCTG 
GACTGCAGTT TTATAATCGA CACACAGGAT AGTGGTGTCA TCAATGGAAA AGCTGAGGAC 
CAGAGTTACT GTCTATCAAA TGGAAAAGAC AATGAGGAAG AGCTGCCACT GCCTAGCTGT 
CCAGAGAGAT GTGGAAGCTC CTCCATCAAA GCCAAGCCTC CGCCAGTCTC CAAGAAACCC 
AAACTTACCT TTGTTCCATC CCAACCAATC AGCGAGCCAG CTCAAACTCA AAATGACAAT 
GCAGCCAGTC TGTCGCCAGA GGATCAGGTC GATGCACAAA CGACAAATGA CGTAACAAAT 
AAGAGGAACA ACCGGGAGGA AGAGGCGTCA GAGAGCACAA AGGCTTCCAC TGAGAACCTG 
GACCAGAGCT GCCAAGATGC AGATCTTGAC CACGACATTT GCACTAATGG AGAGGTGTTT 
GATGAGGATG AAGAAGAGGA AGAAGGAGAA GGAGCAAGCA GCACCACGGG GTCCATCAGC 
TCAAAAGAGG AAGACACAAG TGAAGTTTTC GAATCCAGAC CGGCTGAACC TCCTTCACCC 
CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA ATGGTGACCC CGACTCCCTC CAAACCCCGA 
ACCACTGATG ACCTTTTTGC TGTCATACAC AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA 
GAGTCGGATG AAGACAAGTC CCGCCCCGGG GTTCAGGCAC AGTCTCCACC CTCCACCCCG 
TCACCCGTCT CTCCTGGGCC GGTTTCTCCG TCGGCCCGTC AGATGGGTTC CATCCAGCGA 
AACCTACGCA AGTCCGCCAC CAGCAGCGAC AACTTCAAGG CCCTCCTGCT TCGGAAGGGA 
AGCCGCTCTG AGAACAGTTT CCGCATGTCT GCTACTGAAA TGCTGCGCTC CACCGACCCG 
CGCTTCCAGC GAACTCGCTC CGAGTCTGCG CTGGACCCCC CCACTGCGTC TCCCACGTCA 
CCGATGGCGC CTCACAGCCC CTCTGCCTCT TTTGGGCGGA GTAAGAGGGT AACGGATGAG 
TGGAGCCGCT ACGATGCCCT GTCCTCGCCG ACATATTCGC CTGGATCAAA GTTCGGGCGC 
TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC AAATACAACA CTCGCAGCCG AATCCTCAGC 
AGCCCGATGA CTGTGATCTG TGAGCGTGAA GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG 
GTCGGCGAGA GCCGGGAAGG AGTCCAGACG CTTCCAGAAA CCAACGGCAC TTTATCCGAA 
GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953321.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809089.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa114920
Clone ID Related Accession (Same CDS sequence)	XM_023953321.1
Accession Version	XM_023953321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGGGGCAGT GTTTGACCGC 
CAGGCGAGCA TCCGGCGCTC CCTCATTAAC ACTGATACCG TGTCCCGTCG GCCCAAGAAG 
GTCAAACGCA GAAAGACTAT ATCAGGGCTG CCTGACAACT TCAACCAGGA GCTAGCTAAA 
GGACGAGGCG GGGAACTTCG GCCACATTCC ATGTTCATCC CAGGACAGTA CTCCACTTTG 
GGGAGAGTTG GGAGTGTCAA CTCAACACTC CGCTTCGAGA AAAGAGACTC CAGCTGCCAG 
ACAGAAGAAG TGAAGATTGT CCCTCCATCT GTGAGAAGAA TTCGAGCACA AAGAGGGCAG 
GGAATCGCCG CTCAAATGGC TGGGATATCT GCTTCCTCTT CTACTGGAAG CATCTCCATC 
TCGAGCACAG ATGGCTTGAT TCTGCCCAAT CAGTTTTTCA GAGACCCTTC CCGCTTTCAC 
AGTCTGCCGC GACAGGGTGC CAGAGTGTCC CTCAGTGCTG ATCCCATCTA TAGCAGCACG 
CCCATTAAGT CAGAGGATCA GTTGCAGAGG AATATTGGAA AATTCCAGGT TGACGAAACA 
GCGGTACACA TCAGGAATGC CCCACGAACG AGCACCTTGC CCAGACCCAC GTCTCAGGAG 
GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA GGTGGTCCAG CATGTGTGGT CTCCCCTCAG 
GCAGCTTATT CCACCTCCTT CATTCCCAAT GCCACCCTGT CCAACTCCAC GGATGTTATC 
ACCCTCAGCT CATCTAGTCA TTTATCCTTA TCAGCTGTCT CAGCTTTGGC CACTTCTACC 
CCCACGCGTG CACCACAAGA GAACGGTGTG ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC 
CACTTGGATG GAAGTGTTCA TAGTCACAGT ACTTTGGCTC CCACTTCACC ATCATGTCCT 
CCAGAAGAAC AGTGGATCTA TGACTCACCC GAAAATGTGG TTCCACACCG CACTCTGACC 
TCCAGCTGTT CCACTCCCAT CAACCAGCTT TATAGCAGCC TGGACCATTC CTCACGGACT 
GATTCCAGCT CCCTCTACTC CCAGGACAAC GATGGATACT TCACCTCTAT GCACATGGAC 
TCAGGGCTGC GCTCCCGAAG TCACGGAAGT GGGCACGGCG CAGGAGCTGG GCGGTCCCGA 
CACAGCATGT ATGAGTGTCG CGAGATGGCC AATCGAGAAG ACTCTGGAAG CGTGTACAGC 
GACCGCTCTC TGTCGCGCAG CATCTCGCTC CGCAAGTCAA AGAAGCCACC GCCACCTCCG 
GCACGCACAG ATTCTCTGAG GCGCAAGCCT GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT 
CCAACAACTG TTGCCAATGG GGACGGTGCT AACATGCTCA ATGAAACGTT CCTTGCTAGC 
TTGCAGCAGA GCCTGCAAAT GGGACTGAGG GGGTCAAAGG GAAAGGGTAC CTCTCTATCT 
TCACCCTCTC ACAGCCCCAG CAGTGACTAT GATGACCCTT GGTTGTTGCG ACCGCGCAGT 
CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC GCCTCATTAG CTAACACAAA TGGTGTGTCT 
AATGTCTACT CATTATGCCA TGTCACGCCC ACGCATAGTG ACACCAGCAG TTTGCGTTCT 
GACTATGCAG AGTCCTGGGG ATACTACATG GACTACCCTC GTAACCCTGG AGACCAGGGG 
GGGCAGACCC CACCAGCCCA TGCTACAGAT AACATGTCAG CTGGGGGTCA TCCAGGAGTC 
CTACAGAATG GAGGAGAGAT TCACAGCAAT ACTCAAACAG ATGGAGCTCC AGGCCAGGAG 
GGAGGGCAGT CAGGGAAGCC CAAACCATCC AGCTCCTCAC CGGACAGGGT GCACAGACTT 
ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG TCCAACACGC CTACAGCCGG AACCCCGGTG 
CCAGCCTTCG TCAGATCCAT GTCTCCCTCC TCAGGGAGTC GGCCCAAACC CAAAGTGCCG 
GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC 
TCCAACACTT CAGACTCACT AAAGAGTTCA GGTCCTCCTC TGCCACCCCC TAATCCCCTC 
CATCTCACCT CTTCTACCCC AAACACCCCT CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT 
CTTCCTCCAA ATTCTACATC AGCTCCCTCA TCTCCTCCTC CACCTCCTCC ATTACCCAAC 
TCTCCAAGAG AAACCTTCTT GGCCCCTCTA TCTGCGTCAC CAGAATTCCC TCCTCCTCCA 
TCTCCAGACC TGTTGATGCA CCACAGTTCG TCCTTAAATG GGAGCTTTAG TCCTCCACCT 
CCCCCTGAGA CCGGGATCCC TCCACCACCT CCACTGCCTG ACTTCACCCC AGCTTTTGGA 
TCCCCAAATA TTAGGAAATT GACAAAAAAT ACTCCTAAAC CACATCCTTC CAAACACTCC 
ACAGAGTCCC CCAAGCCCCT CATCACCCCC TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC 
GTCAAGCGAT CACAAGAGGA GATCACTGGG GAGCTGGACC AAATGAAACC TCAGGAAAAT 
GGACTGGACC TTCTCCAGGA GCTGAAACTC CATAGTCTAG ATCCATCCCC CATCCAGGAG 
CACACCACTG TCACGAGTAG CTCTCCAGAC GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT 
GTGACGAAAC TGCTGGAAGA GCTGAGTCTG GACTGCAGTT TTATAATCGA CACACAGGAT 
AGTGGTGTCA TCAATGGAAA AGCTGAGGAC CAGAGTTACT GTCTATCAAA TGGAAAAGAC 
AATGAGGAAG AGCTGCCACT GCCTAGCTGT CCAGAGAGAT GTGGAAGCTC CTCCATCAAA 
GCCAAGCCTC CGCCAGTCTC CAAGAAACCC AAACTTACCT TTGTTCCATC CCAACCAATC 
AGCGAGCCAG CTCAAACTCA AAATGACAAT GCAGCCAGTC TGTCGCCAGA GGATCAGGTC 
GATGCACAAA CGACAAATGA CGTAACAAAT AAGAGGAACA ACCGGGAGGA AGAGGCGTCA 
GAGAGCACAA AGGCTTCCAC TGAGAACCTG GACCAGAGCT GCCAAGATGC AGATCTTGAC 
CACGACATTT GCACTAATGG AGAGGTGTTT GATGAGGATG AAGAAGAGGA AGAAGGAGAA 
GGAGCAAGCA GCACCACGGG GTCCATCAGC TCAAAAGAGG AAGACACAAG TGAAGTTTTC 
GAATCCAGAC CGGCTGAACC TCCTTCACCC CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA 
ATGGTGACCC CGACTCCCTC CAAACCCCGA ACCACTGATG ACCTTTTTGC TGTCATACAC 
AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA GAGTCGGATG AAGACAAGTC CCGCCCCGGG 
GTTCAGGCAC AGTCTCCACC CTCCACCCCG TCACCCGTCT CTCCTGGGCC GGTTTCTCCG 
TCGGCCCGTC AGATGGGTTC CATCCAGCGA AACCTACGCA AGTCCGCCAC CAGCAGCGAC 
AACTTCAAGG CCCTCCTGCT TCGGAAGGGA AGCCGCTCTG AGAACAGTTT CCGCATGTCT 
GCTACTGAAA TGCTGCGCTC CACCGACCCG CGCTTCCAGC GAACTCGCTC CGAGTCTGCG 
CTGGACCCCC CCACTGCGTC TCCCACGTCA CCGATGGCGC CTCACAGCCC CTCTGCCTCT 
TTTGGGCGGA GTAAGAGGGT AACGGATGAG TGGAGCCGCT ACGATGCCCT GTCCTCGCCG 
ACATATTCGC CTGGATCAAA GTTCGGGCGC TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC 
AAATACAACA CTCGCAGCCG AATCCTCAGC AGCCCGATGA CTGTGATCTG TGAGCGTGAA 
GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG GTCGGCGAGA GCCGGGAAGG AGTCCAGACG 
CTTCCAGAAA CCAACGGCAC TTTATCCGAA GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809089.1
CDS	1150..5874
Translation	MKGGRRSASFHRERPAGLSRAFSWLSLSSLSEHRRRIFRSQHELHVPSPSSAHLEGSDRDGDDDSWVYKPQHKMAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953321.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953321.1

ORF Insert Sequence:

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGGGGCAGT GTTTGACCGC 
CAGGCGAGCA TCCGGCGCTC CCTCATTAAC ACTGATACCG TGTCCCGTCG GCCCAAGAAG 
GTCAAACGCA GAAAGACTAT ATCAGGGCTG CCTGACAACT TCAACCAGGA GCTAGCTAAA 
GGACGAGGCG GGGAACTTCG GCCACATTCC ATGTTCATCC CAGGACAGTA CTCCACTTTG 
GGGAGAGTTG GGAGTGTCAA CTCAACACTC CGCTTCGAGA AAAGAGACTC CAGCTGCCAG 
ACAGAAGAAG TGAAGATTGT CCCTCCATCT GTGAGAAGAA TTCGAGCACA AAGAGGGCAG 
GGAATCGCCG CTCAAATGGC TGGGATATCT GCTTCCTCTT CTACTGGAAG CATCTCCATC 
TCGAGCACAG ATGGCTTGAT TCTGCCCAAT CAGTTTTTCA GAGACCCTTC CCGCTTTCAC 
AGTCTGCCGC GACAGGGTGC CAGAGTGTCC CTCAGTGCTG ATCCCATCTA TAGCAGCACG 
CCCATTAAGT CAGAGGATCA GTTGCAGAGG AATATTGGAA AATTCCAGGT TGACGAAACA 
GCGGTACACA TCAGGAATGC CCCACGAACG AGCACCTTGC CCAGACCCAC GTCTCAGGAG 
GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA GGTGGTCCAG CATGTGTGGT CTCCCCTCAG 
GCAGCTTATT CCACCTCCTT CATTCCCAAT GCCACCCTGT CCAACTCCAC GGATGTTATC 
ACCCTCAGCT CATCTAGTCA TTTATCCTTA TCAGCTGTCT CAGCTTTGGC CACTTCTACC 
CCCACGCGTG CACCACAAGA GAACGGTGTG ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC 
CACTTGGATG GAAGTGTTCA TAGTCACAGT ACTTTGGCTC CCACTTCACC ATCATGTCCT 
CCAGAAGAAC AGTGGATCTA TGACTCACCC GAAAATGTGG TTCCACACCG CACTCTGACC 
TCCAGCTGTT CCACTCCCAT CAACCAGCTT TATAGCAGCC TGGACCATTC CTCACGGACT 
GATTCCAGCT CCCTCTACTC CCAGGACAAC GATGGATACT TCACCTCTAT GCACATGGAC 
TCAGGGCTGC GCTCCCGAAG TCACGGAAGT GGGCACGGCG CAGGAGCTGG GCGGTCCCGA 
CACAGCATGT ATGAGTGTCG CGAGATGGCC AATCGAGAAG ACTCTGGAAG CGTGTACAGC 
GACCGCTCTC TGTCGCGCAG CATCTCGCTC CGCAAGTCAA AGAAGCCACC GCCACCTCCG 
GCACGCACAG ATTCTCTGAG GCGCAAGCCT GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT 
CCAACAACTG TTGCCAATGG GGACGGTGCT AACATGCTCA ATGAAACGTT CCTTGCTAGC 
TTGCAGCAGA GCCTGCAAAT GGGACTGAGG GGGTCAAAGG GAAAGGGTAC CTCTCTATCT 
TCACCCTCTC ACAGCCCCAG CAGTGACTAT GATGACCCTT GGTTGTTGCG ACCGCGCAGT 
CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC GCCTCATTAG CTAACACAAA TGGTGTGTCT 
AATGTCTACT CATTATGCCA TGTCACGCCC ACGCATAGTG ACACCAGCAG TTTGCGTTCT 
GACTATGCAG AGTCCTGGGG ATACTACATG GACTACCCTC GTAACCCTGG AGACCAGGGG 
GGGCAGACCC CACCAGCCCA TGCTACAGAT AACATGTCAG CTGGGGGTCA TCCAGGAGTC 
CTACAGAATG GAGGAGAGAT TCACAGCAAT ACTCAAACAG ATGGAGCTCC AGGCCAGGAG 
GGAGGGCAGT CAGGGAAGCC CAAACCATCC AGCTCCTCAC CGGACAGGGT GCACAGACTT 
ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG TCCAACACGC CTACAGCCGG AACCCCGGTG 
CCAGCCTTCG TCAGATCCAT GTCTCCCTCC TCAGGGAGTC GGCCCAAACC CAAAGTGCCG 
GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC 
TCCAACACTT CAGACTCACT AAAGAGTTCA GGTCCTCCTC TGCCACCCCC TAATCCCCTC 
CATCTCACCT CTTCTACCCC AAACACCCCT CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT 
CTTCCTCCAA ATTCTACATC AGCTCCCTCA TCTCCTCCTC CACCTCCTCC ATTACCCAAC 
TCTCCAAGAG AAACCTTCTT GGCCCCTCTA TCTGCGTCAC CAGAATTCCC TCCTCCTCCA 
TCTCCAGACC TGTTGATGCA CCACAGTTCG TCCTTAAATG GGAGCTTTAG TCCTCCACCT 
CCCCCTGAGA CCGGGATCCC TCCACCACCT CCACTGCCTG ACTTCACCCC AGCTTTTGGA 
TCCCCAAATA TTAGGAAATT GACAAAAAAT ACTCCTAAAC CACATCCTTC CAAACACTCC 
ACAGAGTCCC CCAAGCCCCT CATCACCCCC TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC 
GTCAAGCGAT CACAAGAGGA GATCACTGGG GAGCTGGACC AAATGAAACC TCAGGAAAAT 
GGACTGGACC TTCTCCAGGA GCTGAAACTC CATAGTCTAG ATCCATCCCC CATCCAGGAG 
CACACCACTG TCACGAGTAG CTCTCCAGAC GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT 
GTGACGAAAC TGCTGGAAGA GCTGAGTCTG GACTGCAGTT TTATAATCGA CACACAGGAT 
AGTGGTGTCA TCAATGGAAA AGCTGAGGAC CAGAGTTACT GTCTATCAAA TGGAAAAGAC 
AATGAGGAAG AGCTGCCACT GCCTAGCTGT CCAGAGAGAT GTGGAAGCTC CTCCATCAAA 
GCCAAGCCTC CGCCAGTCTC CAAGAAACCC AAACTTACCT TTGTTCCATC CCAACCAATC 
AGCGAGCCAG CTCAAACTCA AAATGACAAT GCAGCCAGTC TGTCGCCAGA GGATCAGGTC 
GATGCACAAA CGACAAATGA CGTAACAAAT AAGAGGAACA ACCGGGAGGA AGAGGCGTCA 
GAGAGCACAA AGGCTTCCAC TGAGAACCTG GACCAGAGCT GCCAAGATGC AGATCTTGAC 
CACGACATTT GCACTAATGG AGAGGTGTTT GATGAGGATG AAGAAGAGGA AGAAGGAGAA 
GGAGCAAGCA GCACCACGGG GTCCATCAGC TCAAAAGAGG AAGACACAAG TGAAGTTTTC 
GAATCCAGAC CGGCTGAACC TCCTTCACCC CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA 
ATGGTGACCC CGACTCCCTC CAAACCCCGA ACCACTGATG ACCTTTTTGC TGTCATACAC 
AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA GAGTCGGATG AAGACAAGTC CCGCCCCGGG 
GTTCAGGCAC AGTCTCCACC CTCCACCCCG TCACCCGTCT CTCCTGGGCC GGTTTCTCCG 
TCGGCCCGTC AGATGGGTTC CATCCAGCGA AACCTACGCA AGTCCGCCAC CAGCAGCGAC 
AACTTCAAGG CCCTCCTGCT TCGGAAGGGA AGCCGCTCTG AGAACAGTTT CCGCATGTCT 
GCTACTGAAA TGCTGCGCTC CACCGACCCG CGCTTCCAGC GAACTCGCTC CGAGTCTGCG 
CTGGACCCCC CCACTGCGTC TCCCACGTCA CCGATGGCGC CTCACAGCCC CTCTGCCTCT 
TTTGGGCGGA GTAAGAGGGT AACGGATGAG TGGAGCCGCT ACGATGCCCT GTCCTCGCCG 
ACATATTCGC CTGGATCAAA GTTCGGGCGC TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC 
AAATACAACA CTCGCAGCCG AATCCTCAGC AGCCCGATGA CTGTGATCTG TGAGCGTGAA 
GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG GTCGGCGAGA GCCGGGAAGG AGTCCAGACG 
CTTCCAGAAA CCAACGGCAC TTTATCCGAA GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953322.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809090.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.

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CloneID	OJa114921
Clone ID Related Accession (Same CDS sequence)	XM_023953322.1
Accession Version	XM_023953322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGCTAAAGGA CGAGGCGGGG AACTTCGGCC ACATTCCATG 
TTCATCCCAG GACAGTACTC CACTTTGGGG AGAGTTGGGA GTGTCAACTC AACACTCCGC 
TTCGAGAAAA GAGACTCCAG CTGCCAGACA GAAGAAGTGA AGATTGTCCC TCCATCTGTG 
AGAAGAATTC GAGCACAAAG AGGGCAGGGA ATCGCCGCTC AAATGGCTGG GATATCTGCT 
TCCTCTTCTA CTGGAAGCAT CTCCATCTCG AGCACAGATG GCTTGATTCT GCCCAATCAG 
TTTTTCAGAG ACCCTTCCCG CTTTCACAGT CTGCCGCGAC AGGGTGCCAG AGTGTCCCTC 
AGTGCTGATC CCATCTATAG CAGCACGCCC ATTAAGTCAG AGGATCAGTT GCAGAGGAAT 
ATTGGAAAAT TCCAGGTTGA CGAAACAGCG GTACACATCA GGAATGCCCC ACGAACGAGC 
ACCTTGCCCA GACCCACGTC TCAGGAGGTG AGGAGGACGC AGTCCAGCGA GTGGGGAGGT 
GGTCCAGCAT GTGTGGTCTC CCCTCAGGCA GCTTATTCCA CCTCCTTCAT TCCCAATGCC 
ACCCTGTCCA ACTCCACGGA TGTTATCACC CTCAGCTCAT CTAGTCATTT ATCCTTATCA 
GCTGTCTCAG CTTTGGCCAC TTCTACCCCC ACGCGTGCAC CACAAGAGAA CGGTGTGACG 
CCTTCAGGCC CCCCCAGTGA GTCGGGCCAC TTGGATGGAA GTGTTCATAG TCACAGTACT 
TTGGCTCCCA CTTCACCATC ATGTCCTCCA GAAGAACAGT GGATCTATGA CTCACCCGAA 
AATGTGGTTC CACACCGCAC TCTGACCTCC AGCTGTTCCA CTCCCATCAA CCAGCTTTAT 
AGCAGCCTGG ACCATTCCTC ACGGACTGAT TCCAGCTCCC TCTACTCCCA GGACAACGAT 
GGATACTTCA CCTCTATGCA CATGGACTCA GGGCTGCGCT CCCGAAGTCA CGGAAGTGGG 
CACGGCGCAG GAGCTGGGCG GTCCCGACAC AGCATGTATG AGTGTCGCGA GATGGCCAAT 
CGAGAAGACT CTGGAAGCGT GTACAGCGAC CGCTCTCTGT CGCGCAGCAT CTCGCTCCGC 
AAGTCAAAGA AGCCACCGCC ACCTCCGGCA CGCACAGATT CTCTGAGGCG CAAGCCTGCC 
GCAAAAAAGC CCTTAGGTGG TGTTGGTCCA ACAACTGTTG CCAATGGGGA CGGTGCTAAC 
ATGCTCAATG AAACGTTCCT TGCTAGCTTG CAGCAGAGCC TGCAAATGGG ACTGAGGGGG 
TCAAAGGGAA AGGGTACCTC TCTATCTTCA CCCTCTCACA GCCCCAGCAG TGACTATGAT 
GACCCTTGGT TGTTGCGACC GCGCAGTCAG AGTAGCATCA GTGCAGGCAG CTCTGCCGCC 
TCATTAGCTA ACACAAATGG TGTGTCTAAT GTCTACTCAT TATGCCATGT CACGCCCACG 
CATAGTGACA CCAGCAGTTT GCGTTCTGAC TATGCAGAGT CCTGGGGATA CTACATGGAC 
TACCCTCGTA ACCCTGGAGA CCAGGGGGGG CAGACCCCAC CAGCCCATGC TACAGATAAC 
ATGTCAGCTG GGGGTCATCC AGGAGTCCTA CAGAATGGAG GAGAGATTCA CAGCAATACT 
CAAACAGATG GAGCTCCAGG CCAGGAGGGA GGGCAGTCAG GGAAGCCCAA ACCATCCAGC 
TCCTCACCGG ACAGGGTGCA CAGACTTACC TCCCCGTCAA GCGGTTACTC CAGCCAGTCC 
AACACGCCTA CAGCCGGAAC CCCGGTGCCA GCCTTCGTCA GATCCATGTC TCCCTCCTCA 
GGGAGTCGGC CCAAACCCAA AGTGCCGGAG AGGAAGTCCT CGCTCCTCTC TTCGGGTTCT 
GTCTCTTCGT CCTCTACTTC TTTGTCCTCC AACACTTCAG ACTCACTAAA GAGTTCAGGT 
CCTCCTCTGC CACCCCCTAA TCCCCTCCAT CTCACCTCTT CTACCCCAAA CACCCCTCTT 
GGTCCTCCTC CGCCCTTTCC TCCTCCTCTT CCTCCAAATT CTACATCAGC TCCCTCATCT 
CCTCCTCCAC CTCCTCCATT ACCCAACTCT CCAAGAGAAA CCTTCTTGGC CCCTCTATCT 
GCGTCACCAG AATTCCCTCC TCCTCCATCT CCAGACCTGT TGATGCACCA CAGTTCGTCC 
TTAAATGGGA GCTTTAGTCC TCCACCTCCC CCTGAGACCG GGATCCCTCC ACCACCTCCA 
CTGCCTGACT TCACCCCAGC TTTTGGATCC CCAAATATTA GGAAATTGAC AAAAAATACT 
CCTAAACCAC ATCCTTCCAA ACACTCCACA GAGTCCCCCA AGCCCCTCAT CACCCCCTTC 
GCCCTGCAGA GTGTCCAGCT GCGCTCCGTC AAGCGATCAC AAGAGGAGAT CACTGGGGAG 
CTGGACCAAA TGAAACCTCA GGAAAATGGA CTGGACCTTC TCCAGGAGCT GAAACTCCAT 
AGTCTAGATC CATCCCCCAT CCAGGAGCAC ACCACTGTCA CGAGTAGCTC TCCAGACGAA 
GAGCTGCGGG AGTCCACACC TTCGCCTGTG ACGAAACTGC TGGAAGAGCT GAGTCTGGAC 
TGCAGTTTTA TAATCGACAC ACAGGATAGT GGTGTCATCA ATGGAAAAGC TGAGGACCAG 
AGTTACTGTC TATCAAATGG AAAAGACAAT GAGGAAGAGC TGCCACTGCC TAGCTGTCCA 
GAGAGATGTG GAAGCTCCTC CATCAAAGCC AAGCCTCCGC CAGTCTCCAA GAAACCCAAA 
CTTACCTTTG TTCCATCCCA ACCAATCAGC GAGCCAGCTC AAACTCAAAA TGACAATGCA 
GCCAGTCTGT CGCCAGAGGA TCAGGTCGAT GCACAAACGA CAAATGACGT AACAAATAAG 
AGGAACAACC GGGAGGAAGA GGCGTCAGAG AGCACAAAGG CTTCCACTGA GAACCTGGAC 
CAGAGCTGCC AAGATGCAGA TCTTGACCAC GACATTTGCA CTAATGGAGA GGTGTTTGAT 
GAGGATGAAG AAGAGGAAGA AGGAGAAGGA GCAAGCAGCA CCACGGGGTC CATCAGCTCA 
AAAGAGGAAG ACACAAGTGA AGTTTTCGAA TCCAGACCGG CTGAACCTCC TTCACCCCAG 
TCGGCCGTAG GGGCAAGCGA GCAAAAAATG GTGACCCCGA CTCCCTCCAA ACCCCGAACC 
ACTGATGACC TTTTTGCTGT CATACACAGG TCAAAGCGAA AGGTTCTCGG TCGCAGAGAG 
TCGGATGAAG ACAAGTCCCG CCCCGGGGTT CAGGCACAGT CTCCACCCTC CACCCCGTCA 
CCCGTCTCTC CTGGGCCGGT TTCTCCGTCG GCCCGTCAGA TGGGTTCCAT CCAGCGAAAC 
CTACGCAAGT CCGCCACCAG CAGCGACAAC TTCAAGGCCC TCCTGCTTCG GAAGGGAAGC 
CGCTCTGAGA ACAGTTTCCG CATGTCTGCT ACTGAAATGC TGCGCTCCAC CGACCCGCGC 
TTCCAGCGAA CTCGCTCCGA GTCTGCGCTG GACCCCCCCA CTGCGTCTCC CACGTCACCG 
ATGGCGCCTC ACAGCCCCTC TGCCTCTTTT GGGCGGAGTA AGAGGGTAAC GGATGAGTGG 
AGCCGCTACG ATGCCCTGTC CTCGCCGACA TATTCGCCTG GATCAAAGTT CGGGCGCTCT 
CGCGCGCCGC CTTCCGCCGC CAGCAGCAAA TACAACACTC GCAGCCGAAT CCTCAGCAGC 
CCGATGACTG TGATCTGTGA GCGTGAAGGG GAGCTGACTG AGAGCGAATC TGGAGAGGTC 
GGCGAGAGCC GGGAAGGAGT CCAGACGCTT CCAGAAACCA ACGGCACTTT ATCCGAAGAG 
AGCAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809090.1
CDS	329..5020
Translation	MPFHQRTVEPRRVSRLSARYGWKAREEEPGKRKTRRLVLFSSLDEVSCHTLTGLIHQLSDLSRHASDIFLGIEVEAGAVFRRSCRIQGRLQFLQDEVCRLDPKKVKIPVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953322.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953322.1

ORF Insert Sequence:

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGCTAAAGGA CGAGGCGGGG AACTTCGGCC ACATTCCATG 
TTCATCCCAG GACAGTACTC CACTTTGGGG AGAGTTGGGA GTGTCAACTC AACACTCCGC 
TTCGAGAAAA GAGACTCCAG CTGCCAGACA GAAGAAGTGA AGATTGTCCC TCCATCTGTG 
AGAAGAATTC GAGCACAAAG AGGGCAGGGA ATCGCCGCTC AAATGGCTGG GATATCTGCT 
TCCTCTTCTA CTGGAAGCAT CTCCATCTCG AGCACAGATG GCTTGATTCT GCCCAATCAG 
TTTTTCAGAG ACCCTTCCCG CTTTCACAGT CTGCCGCGAC AGGGTGCCAG AGTGTCCCTC 
AGTGCTGATC CCATCTATAG CAGCACGCCC ATTAAGTCAG AGGATCAGTT GCAGAGGAAT 
ATTGGAAAAT TCCAGGTTGA CGAAACAGCG GTACACATCA GGAATGCCCC ACGAACGAGC 
ACCTTGCCCA GACCCACGTC TCAGGAGGTG AGGAGGACGC AGTCCAGCGA GTGGGGAGGT 
GGTCCAGCAT GTGTGGTCTC CCCTCAGGCA GCTTATTCCA CCTCCTTCAT TCCCAATGCC 
ACCCTGTCCA ACTCCACGGA TGTTATCACC CTCAGCTCAT CTAGTCATTT ATCCTTATCA 
GCTGTCTCAG CTTTGGCCAC TTCTACCCCC ACGCGTGCAC CACAAGAGAA CGGTGTGACG 
CCTTCAGGCC CCCCCAGTGA GTCGGGCCAC TTGGATGGAA GTGTTCATAG TCACAGTACT 
TTGGCTCCCA CTTCACCATC ATGTCCTCCA GAAGAACAGT GGATCTATGA CTCACCCGAA 
AATGTGGTTC CACACCGCAC TCTGACCTCC AGCTGTTCCA CTCCCATCAA CCAGCTTTAT 
AGCAGCCTGG ACCATTCCTC ACGGACTGAT TCCAGCTCCC TCTACTCCCA GGACAACGAT 
GGATACTTCA CCTCTATGCA CATGGACTCA GGGCTGCGCT CCCGAAGTCA CGGAAGTGGG 
CACGGCGCAG GAGCTGGGCG GTCCCGACAC AGCATGTATG AGTGTCGCGA GATGGCCAAT 
CGAGAAGACT CTGGAAGCGT GTACAGCGAC CGCTCTCTGT CGCGCAGCAT CTCGCTCCGC 
AAGTCAAAGA AGCCACCGCC ACCTCCGGCA CGCACAGATT CTCTGAGGCG CAAGCCTGCC 
GCAAAAAAGC CCTTAGGTGG TGTTGGTCCA ACAACTGTTG CCAATGGGGA CGGTGCTAAC 
ATGCTCAATG AAACGTTCCT TGCTAGCTTG CAGCAGAGCC TGCAAATGGG ACTGAGGGGG 
TCAAAGGGAA AGGGTACCTC TCTATCTTCA CCCTCTCACA GCCCCAGCAG TGACTATGAT 
GACCCTTGGT TGTTGCGACC GCGCAGTCAG AGTAGCATCA GTGCAGGCAG CTCTGCCGCC 
TCATTAGCTA ACACAAATGG TGTGTCTAAT GTCTACTCAT TATGCCATGT CACGCCCACG 
CATAGTGACA CCAGCAGTTT GCGTTCTGAC TATGCAGAGT CCTGGGGATA CTACATGGAC 
TACCCTCGTA ACCCTGGAGA CCAGGGGGGG CAGACCCCAC CAGCCCATGC TACAGATAAC 
ATGTCAGCTG GGGGTCATCC AGGAGTCCTA CAGAATGGAG GAGAGATTCA CAGCAATACT 
CAAACAGATG GAGCTCCAGG CCAGGAGGGA GGGCAGTCAG GGAAGCCCAA ACCATCCAGC 
TCCTCACCGG ACAGGGTGCA CAGACTTACC TCCCCGTCAA GCGGTTACTC CAGCCAGTCC 
AACACGCCTA CAGCCGGAAC CCCGGTGCCA GCCTTCGTCA GATCCATGTC TCCCTCCTCA 
GGGAGTCGGC CCAAACCCAA AGTGCCGGAG AGGAAGTCCT CGCTCCTCTC TTCGGGTTCT 
GTCTCTTCGT CCTCTACTTC TTTGTCCTCC AACACTTCAG ACTCACTAAA GAGTTCAGGT 
CCTCCTCTGC CACCCCCTAA TCCCCTCCAT CTCACCTCTT CTACCCCAAA CACCCCTCTT 
GGTCCTCCTC CGCCCTTTCC TCCTCCTCTT CCTCCAAATT CTACATCAGC TCCCTCATCT 
CCTCCTCCAC CTCCTCCATT ACCCAACTCT CCAAGAGAAA CCTTCTTGGC CCCTCTATCT 
GCGTCACCAG AATTCCCTCC TCCTCCATCT CCAGACCTGT TGATGCACCA CAGTTCGTCC 
TTAAATGGGA GCTTTAGTCC TCCACCTCCC CCTGAGACCG GGATCCCTCC ACCACCTCCA 
CTGCCTGACT TCACCCCAGC TTTTGGATCC CCAAATATTA GGAAATTGAC AAAAAATACT 
CCTAAACCAC ATCCTTCCAA ACACTCCACA GAGTCCCCCA AGCCCCTCAT CACCCCCTTC 
GCCCTGCAGA GTGTCCAGCT GCGCTCCGTC AAGCGATCAC AAGAGGAGAT CACTGGGGAG 
CTGGACCAAA TGAAACCTCA GGAAAATGGA CTGGACCTTC TCCAGGAGCT GAAACTCCAT 
AGTCTAGATC CATCCCCCAT CCAGGAGCAC ACCACTGTCA CGAGTAGCTC TCCAGACGAA 
GAGCTGCGGG AGTCCACACC TTCGCCTGTG ACGAAACTGC TGGAAGAGCT GAGTCTGGAC 
TGCAGTTTTA TAATCGACAC ACAGGATAGT GGTGTCATCA ATGGAAAAGC TGAGGACCAG 
AGTTACTGTC TATCAAATGG AAAAGACAAT GAGGAAGAGC TGCCACTGCC TAGCTGTCCA 
GAGAGATGTG GAAGCTCCTC CATCAAAGCC AAGCCTCCGC CAGTCTCCAA GAAACCCAAA 
CTTACCTTTG TTCCATCCCA ACCAATCAGC GAGCCAGCTC AAACTCAAAA TGACAATGCA 
GCCAGTCTGT CGCCAGAGGA TCAGGTCGAT GCACAAACGA CAAATGACGT AACAAATAAG 
AGGAACAACC GGGAGGAAGA GGCGTCAGAG AGCACAAAGG CTTCCACTGA GAACCTGGAC 
CAGAGCTGCC AAGATGCAGA TCTTGACCAC GACATTTGCA CTAATGGAGA GGTGTTTGAT 
GAGGATGAAG AAGAGGAAGA AGGAGAAGGA GCAAGCAGCA CCACGGGGTC CATCAGCTCA 
AAAGAGGAAG ACACAAGTGA AGTTTTCGAA TCCAGACCGG CTGAACCTCC TTCACCCCAG 
TCGGCCGTAG GGGCAAGCGA GCAAAAAATG GTGACCCCGA CTCCCTCCAA ACCCCGAACC 
ACTGATGACC TTTTTGCTGT CATACACAGG TCAAAGCGAA AGGTTCTCGG TCGCAGAGAG 
TCGGATGAAG ACAAGTCCCG CCCCGGGGTT CAGGCACAGT CTCCACCCTC CACCCCGTCA 
CCCGTCTCTC CTGGGCCGGT TTCTCCGTCG GCCCGTCAGA TGGGTTCCAT CCAGCGAAAC 
CTACGCAAGT CCGCCACCAG CAGCGACAAC TTCAAGGCCC TCCTGCTTCG GAAGGGAAGC 
CGCTCTGAGA ACAGTTTCCG CATGTCTGCT ACTGAAATGC TGCGCTCCAC CGACCCGCGC 
TTCCAGCGAA CTCGCTCCGA GTCTGCGCTG GACCCCCCCA CTGCGTCTCC CACGTCACCG 
ATGGCGCCTC ACAGCCCCTC TGCCTCTTTT GGGCGGAGTA AGAGGGTAAC GGATGAGTGG 
AGCCGCTACG ATGCCCTGTC CTCGCCGACA TATTCGCCTG GATCAAAGTT CGGGCGCTCT 
CGCGCGCCGC CTTCCGCCGC CAGCAGCAAA TACAACACTC GCAGCCGAAT CCTCAGCAGC 
CCGATGACTG TGATCTGTGA GCGTGAAGGG GAGCTGACTG AGAGCGAATC TGGAGAGGTC 
GGCGAGAGCC GGGAAGGAGT CCAGACGCTT CCAGAAACCA ACGGCACTTT ATCCGAAGAG 
AGCAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953323.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809091.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.

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CloneID	OJa114922
Clone ID Related Accession (Same CDS sequence)	XM_023953323.1
Accession Version	XM_023953323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGCTAAAGG ACGAGGCGGG 
GAACTTCGGC CACATTCCAT GTTCATCCCA GGACAGTACT CCACTTTGGG GAGAGTTGGG 
AGTGTCAACT CAACACTCCG CTTCGAGAAA AGAGACTCCA GCTGCCAGAC AGAAGAAGTG 
AAGATTGTCC CTCCATCTGT GAGAAGAATT CGAGCACAAA GAGGGCAGGG AATCGCCGCT 
CAAATGGCTG GGATATCTGC TTCCTCTTCT ACTGGAAGCA TCTCCATCTC GAGCACAGAT 
GGCTTGATTC TGCCCAATCA GTTTTTCAGA GACCCTTCCC GCTTTCACAG TCTGCCGCGA 
CAGGGTGCCA GAGTGTCCCT CAGTGCTGAT CCCATCTATA GCAGCACGCC CATTAAGTCA 
GAGGATCAGT TGCAGAGGAA TATTGGAAAA TTCCAGGTTG ACGAAACAGC GGTACACATC 
AGGAATGCCC CACGAACGAG CACCTTGCCC AGACCCACGT CTCAGGAGGT GAGGAGGACG 
CAGTCCAGCG AGTGGGGAGG TGGTCCAGCA TGTGTGGTCT CCCCTCAGGC AGCTTATTCC 
ACCTCCTTCA TTCCCAATGC CACCCTGTCC AACTCCACGG ATGTTATCAC CCTCAGCTCA 
TCTAGTCATT TATCCTTATC AGCTGTCTCA GCTTTGGCCA CTTCTACCCC CACGCGTGCA 
CCACAAGAGA ACGGTGTGAC GCCTTCAGGC CCCCCCAGTG AGTCGGGCCA CTTGGATGGA 
AGTGTTCATA GTCACAGTAC TTTGGCTCCC ACTTCACCAT CATGTCCTCC AGAAGAACAG 
TGGATCTATG ACTCACCCGA AAATGTGGTT CCACACCGCA CTCTGACCTC CAGCTGTTCC 
ACTCCCATCA ACCAGCTTTA TAGCAGCCTG GACCATTCCT CACGGACTGA TTCCAGCTCC 
CTCTACTCCC AGGACAACGA TGGATACTTC ACCTCTATGC ACATGGACTC AGGGCTGCGC 
TCCCGAAGTC ACGGAAGTGG GCACGGCGCA GGAGCTGGGC GGTCCCGACA CAGCATGTAT 
GAGTGTCGCG AGATGGCCAA TCGAGAAGAC TCTGGAAGCG TGTACAGCGA CCGCTCTCTG 
TCGCGCAGCA TCTCGCTCCG CAAGTCAAAG AAGCCACCGC CACCTCCGGC ACGCACAGAT 
TCTCTGAGGC GCAAGCCTGC CGCAAAAAAG CCCTTAGGTG GTGTTGGTCC AACAACTGTT 
GCCAATGGGG ACGGTGCTAA CATGCTCAAT GAAACGTTCC TTGCTAGCTT GCAGCAGAGC 
CTGCAAATGG GACTGAGGGG GTCAAAGGGA AAGGGTACCT CTCTATCTTC ACCCTCTCAC 
AGCCCCAGCA GTGACTATGA TGACCCTTGG TTGTTGCGAC CGCGCAGTCA GAGTAGCATC 
AGTGCAGGCA GCTCTGCCGC CTCATTAGCT AACACAAATG GTGTGTCTAA TGTCTACTCA 
TTATGCCATG TCACGCCCAC GCATAGTGAC ACCAGCAGTT TGCGTTCTGA CTATGCAGAG 
TCCTGGGGAT ACTACATGGA CTACCCTCGT AACCCTGGAG ACCAGGGGGG GCAGACCCCA 
CCAGCCCATG CTACAGATAA CATGTCAGCT GGGGGTCATC CAGGAGTCCT ACAGAATGGA 
GGAGAGATTC ACAGCAATAC TCAAACAGAT GGAGCTCCAG GCCAGGAGGG AGGGCAGTCA 
GGGAAGCCCA AACCATCCAG CTCCTCACCG GACAGGGTGC ACAGACTTAC CTCCCCGTCA 
AGCGGTTACT CCAGCCAGTC CAACACGCCT ACAGCCGGAA CCCCGGTGCC AGCCTTCGTC 
AGATCCATGT CTCCCTCCTC AGGGAGTCGG CCCAAACCCA AAGTGCCGGA GAGGAAGTCC 
TCGCTCCTCT CTTCGGGTTC TGTCTCTTCG TCCTCTACTT CTTTGTCCTC CAACACTTCA 
GACTCACTAA AGAGTTCAGG TCCTCCTCTG CCACCCCCTA ATCCCCTCCA TCTCACCTCT 
TCTACCCCAA ACACCCCTCT TGGTCCTCCT CCGCCCTTTC CTCCTCCTCT TCCTCCAAAT 
TCTACATCAG CTCCCTCATC TCCTCCTCCA CCTCCTCCAT TACCCAACTC TCCAAGAGAA 
ACCTTCTTGG CCCCTCTATC TGCGTCACCA GAATTCCCTC CTCCTCCATC TCCAGACCTG 
TTGATGCACC ACAGTTCGTC CTTAAATGGG AGCTTTAGTC CTCCACCTCC CCCTGAGACC 
GGGATCCCTC CACCACCTCC ACTGCCTGAC TTCACCCCAG CTTTTGGATC CCCAAATATT 
AGGAAATTGA CAAAAAATAC TCCTAAACCA CATCCTTCCA AACACTCCAC AGAGTCCCCC 
AAGCCCCTCA TCACCCCCTT CGCCCTGCAG AGTGTCCAGC TGCGCTCCGT CAAGCGATCA 
CAAGAGGAGA TCACTGGGGA GCTGGACCAA ATGAAACCTC AGGAAAATGG ACTGGACCTT 
CTCCAGGAGC TGAAACTCCA TAGTCTAGAT CCATCCCCCA TCCAGGAGCA CACCACTGTC 
ACGAGTAGCT CTCCAGACGA AGAGCTGCGG GAGTCCACAC CTTCGCCTGT GACGAAACTG 
CTGGAAGAGC TGAGTCTGGA CTGCAGTTTT ATAATCGACA CACAGGATAG TGGTGTCATC 
AATGGAAAAG CTGAGGACCA GAGTTACTGT CTATCAAATG GAAAAGACAA TGAGGAAGAG 
CTGCCACTGC CTAGCTGTCC AGAGAGATGT GGAAGCTCCT CCATCAAAGC CAAGCCTCCG 
CCAGTCTCCA AGAAACCCAA ACTTACCTTT GTTCCATCCC AACCAATCAG CGAGCCAGCT 
CAAACTCAAA ATGACAATGC AGCCAGTCTG TCGCCAGAGG ATCAGGTCGA TGCACAAACG 
ACAAATGACG TAACAAATAA GAGGAACAAC CGGGAGGAAG AGGCGTCAGA GAGCACAAAG 
GCTTCCACTG AGAACCTGGA CCAGAGCTGC CAAGATGCAG ATCTTGACCA CGACATTTGC 
ACTAATGGAG AGGTGTTTGA TGAGGATGAA GAAGAGGAAG AAGGAGAAGG AGCAAGCAGC 
ACCACGGGGT CCATCAGCTC AAAAGAGGAA GACACAAGTG AAGTTTTCGA ATCCAGACCG 
GCTGAACCTC CTTCACCCCA GTCGGCCGTA GGGGCAAGCG AGCAAAAAAT GGTGACCCCG 
ACTCCCTCCA AACCCCGAAC CACTGATGAC CTTTTTGCTG TCATACACAG GTCAAAGCGA 
AAGGTTCTCG GTCGCAGAGA GTCGGATGAA GACAAGTCCC GCCCCGGGGT TCAGGCACAG 
TCTCCACCCT CCACCCCGTC ACCCGTCTCT CCTGGGCCGG TTTCTCCGTC GGCCCGTCAG 
ATGGGTTCCA TCCAGCGAAA CCTACGCAAG TCCGCCACCA GCAGCGACAA CTTCAAGGCC 
CTCCTGCTTC GGAAGGGAAG CCGCTCTGAG AACAGTTTCC GCATGTCTGC TACTGAAATG 
CTGCGCTCCA CCGACCCGCG CTTCCAGCGA ACTCGCTCCG AGTCTGCGCT GGACCCCCCC 
ACTGCGTCTC CCACGTCACC GATGGCGCCT CACAGCCCCT CTGCCTCTTT TGGGCGGAGT 
AAGAGGGTAA CGGATGAGTG GAGCCGCTAC GATGCCCTGT CCTCGCCGAC ATATTCGCCT 
GGATCAAAGT TCGGGCGCTC TCGCGCGCCG CCTTCCGCCG CCAGCAGCAA ATACAACACT 
CGCAGCCGAA TCCTCAGCAG CCCGATGACT GTGATCTGTG AGCGTGAAGG GGAGCTGACT 
GAGAGCGAAT CTGGAGAGGT CGGCGAGAGC CGGGAAGGAG TCCAGACGCT TCCAGAAACC 
AACGGCACTT TATCCGAAGA GAGCAGAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809091.1
CDS	1150..5742
Translation	MKGGRRSASFHRERPAGLSRAFSWLSLSSLSEHRRRIFRSQHELHVPSPSSAHLEGSDRDGDDDSWVYKPQHKMAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953323.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953323.1

ORF Insert Sequence:

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGCTAAAGG ACGAGGCGGG 
GAACTTCGGC CACATTCCAT GTTCATCCCA GGACAGTACT CCACTTTGGG GAGAGTTGGG 
AGTGTCAACT CAACACTCCG CTTCGAGAAA AGAGACTCCA GCTGCCAGAC AGAAGAAGTG 
AAGATTGTCC CTCCATCTGT GAGAAGAATT CGAGCACAAA GAGGGCAGGG AATCGCCGCT 
CAAATGGCTG GGATATCTGC TTCCTCTTCT ACTGGAAGCA TCTCCATCTC GAGCACAGAT 
GGCTTGATTC TGCCCAATCA GTTTTTCAGA GACCCTTCCC GCTTTCACAG TCTGCCGCGA 
CAGGGTGCCA GAGTGTCCCT CAGTGCTGAT CCCATCTATA GCAGCACGCC CATTAAGTCA 
GAGGATCAGT TGCAGAGGAA TATTGGAAAA TTCCAGGTTG ACGAAACAGC GGTACACATC 
AGGAATGCCC CACGAACGAG CACCTTGCCC AGACCCACGT CTCAGGAGGT GAGGAGGACG 
CAGTCCAGCG AGTGGGGAGG TGGTCCAGCA TGTGTGGTCT CCCCTCAGGC AGCTTATTCC 
ACCTCCTTCA TTCCCAATGC CACCCTGTCC AACTCCACGG ATGTTATCAC CCTCAGCTCA 
TCTAGTCATT TATCCTTATC AGCTGTCTCA GCTTTGGCCA CTTCTACCCC CACGCGTGCA 
CCACAAGAGA ACGGTGTGAC GCCTTCAGGC CCCCCCAGTG AGTCGGGCCA CTTGGATGGA 
AGTGTTCATA GTCACAGTAC TTTGGCTCCC ACTTCACCAT CATGTCCTCC AGAAGAACAG 
TGGATCTATG ACTCACCCGA AAATGTGGTT CCACACCGCA CTCTGACCTC CAGCTGTTCC 
ACTCCCATCA ACCAGCTTTA TAGCAGCCTG GACCATTCCT CACGGACTGA TTCCAGCTCC 
CTCTACTCCC AGGACAACGA TGGATACTTC ACCTCTATGC ACATGGACTC AGGGCTGCGC 
TCCCGAAGTC ACGGAAGTGG GCACGGCGCA GGAGCTGGGC GGTCCCGACA CAGCATGTAT 
GAGTGTCGCG AGATGGCCAA TCGAGAAGAC TCTGGAAGCG TGTACAGCGA CCGCTCTCTG 
TCGCGCAGCA TCTCGCTCCG CAAGTCAAAG AAGCCACCGC CACCTCCGGC ACGCACAGAT 
TCTCTGAGGC GCAAGCCTGC CGCAAAAAAG CCCTTAGGTG GTGTTGGTCC AACAACTGTT 
GCCAATGGGG ACGGTGCTAA CATGCTCAAT GAAACGTTCC TTGCTAGCTT GCAGCAGAGC 
CTGCAAATGG GACTGAGGGG GTCAAAGGGA AAGGGTACCT CTCTATCTTC ACCCTCTCAC 
AGCCCCAGCA GTGACTATGA TGACCCTTGG TTGTTGCGAC CGCGCAGTCA GAGTAGCATC 
AGTGCAGGCA GCTCTGCCGC CTCATTAGCT AACACAAATG GTGTGTCTAA TGTCTACTCA 
TTATGCCATG TCACGCCCAC GCATAGTGAC ACCAGCAGTT TGCGTTCTGA CTATGCAGAG 
TCCTGGGGAT ACTACATGGA CTACCCTCGT AACCCTGGAG ACCAGGGGGG GCAGACCCCA 
CCAGCCCATG CTACAGATAA CATGTCAGCT GGGGGTCATC CAGGAGTCCT ACAGAATGGA 
GGAGAGATTC ACAGCAATAC TCAAACAGAT GGAGCTCCAG GCCAGGAGGG AGGGCAGTCA 
GGGAAGCCCA AACCATCCAG CTCCTCACCG GACAGGGTGC ACAGACTTAC CTCCCCGTCA 
AGCGGTTACT CCAGCCAGTC CAACACGCCT ACAGCCGGAA CCCCGGTGCC AGCCTTCGTC 
AGATCCATGT CTCCCTCCTC AGGGAGTCGG CCCAAACCCA AAGTGCCGGA GAGGAAGTCC 
TCGCTCCTCT CTTCGGGTTC TGTCTCTTCG TCCTCTACTT CTTTGTCCTC CAACACTTCA 
GACTCACTAA AGAGTTCAGG TCCTCCTCTG CCACCCCCTA ATCCCCTCCA TCTCACCTCT 
TCTACCCCAA ACACCCCTCT TGGTCCTCCT CCGCCCTTTC CTCCTCCTCT TCCTCCAAAT 
TCTACATCAG CTCCCTCATC TCCTCCTCCA CCTCCTCCAT TACCCAACTC TCCAAGAGAA 
ACCTTCTTGG CCCCTCTATC TGCGTCACCA GAATTCCCTC CTCCTCCATC TCCAGACCTG 
TTGATGCACC ACAGTTCGTC CTTAAATGGG AGCTTTAGTC CTCCACCTCC CCCTGAGACC 
GGGATCCCTC CACCACCTCC ACTGCCTGAC TTCACCCCAG CTTTTGGATC CCCAAATATT 
AGGAAATTGA CAAAAAATAC TCCTAAACCA CATCCTTCCA AACACTCCAC AGAGTCCCCC 
AAGCCCCTCA TCACCCCCTT CGCCCTGCAG AGTGTCCAGC TGCGCTCCGT CAAGCGATCA 
CAAGAGGAGA TCACTGGGGA GCTGGACCAA ATGAAACCTC AGGAAAATGG ACTGGACCTT 
CTCCAGGAGC TGAAACTCCA TAGTCTAGAT CCATCCCCCA TCCAGGAGCA CACCACTGTC 
ACGAGTAGCT CTCCAGACGA AGAGCTGCGG GAGTCCACAC CTTCGCCTGT GACGAAACTG 
CTGGAAGAGC TGAGTCTGGA CTGCAGTTTT ATAATCGACA CACAGGATAG TGGTGTCATC 
AATGGAAAAG CTGAGGACCA GAGTTACTGT CTATCAAATG GAAAAGACAA TGAGGAAGAG 
CTGCCACTGC CTAGCTGTCC AGAGAGATGT GGAAGCTCCT CCATCAAAGC CAAGCCTCCG 
CCAGTCTCCA AGAAACCCAA ACTTACCTTT GTTCCATCCC AACCAATCAG CGAGCCAGCT 
CAAACTCAAA ATGACAATGC AGCCAGTCTG TCGCCAGAGG ATCAGGTCGA TGCACAAACG 
ACAAATGACG TAACAAATAA GAGGAACAAC CGGGAGGAAG AGGCGTCAGA GAGCACAAAG 
GCTTCCACTG AGAACCTGGA CCAGAGCTGC CAAGATGCAG ATCTTGACCA CGACATTTGC 
ACTAATGGAG AGGTGTTTGA TGAGGATGAA GAAGAGGAAG AAGGAGAAGG AGCAAGCAGC 
ACCACGGGGT CCATCAGCTC AAAAGAGGAA GACACAAGTG AAGTTTTCGA ATCCAGACCG 
GCTGAACCTC CTTCACCCCA GTCGGCCGTA GGGGCAAGCG AGCAAAAAAT GGTGACCCCG 
ACTCCCTCCA AACCCCGAAC CACTGATGAC CTTTTTGCTG TCATACACAG GTCAAAGCGA 
AAGGTTCTCG GTCGCAGAGA GTCGGATGAA GACAAGTCCC GCCCCGGGGT TCAGGCACAG 
TCTCCACCCT CCACCCCGTC ACCCGTCTCT CCTGGGCCGG TTTCTCCGTC GGCCCGTCAG 
ATGGGTTCCA TCCAGCGAAA CCTACGCAAG TCCGCCACCA GCAGCGACAA CTTCAAGGCC 
CTCCTGCTTC GGAAGGGAAG CCGCTCTGAG AACAGTTTCC GCATGTCTGC TACTGAAATG 
CTGCGCTCCA CCGACCCGCG CTTCCAGCGA ACTCGCTCCG AGTCTGCGCT GGACCCCCCC 
ACTGCGTCTC CCACGTCACC GATGGCGCCT CACAGCCCCT CTGCCTCTTT TGGGCGGAGT 
AAGAGGGTAA CGGATGAGTG GAGCCGCTAC GATGCCCTGT CCTCGCCGAC ATATTCGCCT 
GGATCAAAGT TCGGGCGCTC TCGCGCGCCG CCTTCCGCCG CCAGCAGCAA ATACAACACT 
CGCAGCCGAA TCCTCAGCAG CCCGATGACT GTGATCTGTG AGCGTGAAGG GGAGCTGACT 
GAGAGCGAAT CTGGAGAGGT CGGCGAGAGC CGGGAAGGAG TCCAGACGCT TCCAGAAACC 
AACGGCACTT TATCCGAAGA GAGCAGAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953324.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809092.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OJa114923
Clone ID Related Accession (Same CDS sequence)	XM_023953324.1
Accession Version	XM_023953324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTA TCGGGACCTC GTTAAAGTCT ATAATTAAGT ACTTTAAAAG AAAGGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGAAGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809092.1
CDS	143..4699
Translation	MVFIGTSLKSIIKYFKRKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953324.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953324.1

ORF Insert Sequence:

ATGGTGTTTA TCGGGACCTC GTTAAAGTCT ATAATTAAGT ACTTTAAAAG AAAGGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGAAGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953325.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809093.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OJa114924
Clone ID Related Accession (Same CDS sequence)	XM_023953325.1
Accession Version	XM_023953325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TCCTGCATCC TCGATCACCT ACTGCGGCGT GGTTACAATC ACTCGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGAAGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809093.1
CDS	44..4600
Translation	MEVVLHPRSPTAAWLQSLAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953325.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953325.1

ORF Insert Sequence:

ATGGAAGTAG TCCTGCATCC TCGATCACCT ACTGCGGCGT GGTTACAATC ACTCGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGAAGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_023953326.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023809094.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.

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CloneID	OJa114925
Clone ID Related Accession (Same CDS sequence)	XM_023953326.1
Accession Version	XM_023953326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTC TGAAAGCTGT GTCAAACCTG GATGAGGAGA GCAAATGGAC GGTACACTAC 
ACGGCTCCTT GGCACCAGCA GGAAAACGTC TTCCTGCCGG GCAGCAGGCC GTCCTGCGTG 
GAGGACCTCC ACCGCCAAGC CAAAGTCAAC CTCAAGACCA CCCTGCGAGA ATGTGATAAG 
TTGAGGAAAG ATGGTTTCCG GAGCTCCCAG TACTACTCTC AGGGCCCGAC CTTCTCCGAG 
CCCTTACAGT CCAGCAGCAG CCTGCAAGAC GAGGAAGATG AGGAAAATGA CAAGAAGTCT 
TTAGCATCCT CGATGGAGGA TGATAAATCC CAGCTCTCGC TGAGGTTGCA GGAGCGCTGC 
GGTGAAAGGG GCGAGATGTT GGAAGTTGAC GGGCAGATGG TGTGGAATAA AGCGGTTCCC 
CTCCCGACGC CGGAGGAGAG GATGAGGCAA GCAGCTCAGG CCGTGCCCAC GGACATAGTC 
CCCATCAATG TCACAGGGGC AGTGTTTGAC CGCCAGGCGA GCATCCGGCG CTCCCTCATT 
AACACTGATA CCGTGTCCCG TCGGCCCAAG AAGGTCAAAC GCAGAAAGAC TATATCAGGG 
CTGCCTGACA ACTTCAACCA GGAGCTAGCT AAAGGACGAG GCGGGGAACT TCGGCCACAT 
TCCATGTTCA TCCCAGGACA GTACTCCACT TTGGGGAGAG TTGGGAGTGT CAACTCAACA 
CTCCGCTTCG AGAAAAGAGA CTCCAGCTGC CAGACAGAAG AAGTGAAGAT TGTCCCTCCA 
TCTGTGAGAA GAATTCGAGC ACAAAGAGGG CAGGGAATCG CCGCTCAAAT GGCTGGGATA 
TCTGCTTCCT CTTCTACTGG AAGCATCTCC ATCTCGAGCA CAGATGGCTT GATTCTGCCC 
AATCAGTTTT TCAGAGACCC TTCCCGCTTT CACAGTCTGC CGCGACAGGG TGCCAGAGTG 
TCCCTCAGTG CTGATCCCAT CTATAGCAGC ACGCCCATTA AGTCAGAGGA TCAGTTGCAG 
AGGAATATTG GAAAATTCCA GGTTGACGAA ACAGCGGTAC ACATCAGGAA TGCCCCACGA 
ACGAGCACCT TGCCCAGACC CACGTCTCAG GAGGTGAGGA GGACGCAGTC CAGCGAGTGG 
GGAGGTGGTC CAGCATGTGT GGTCTCCCCT CAGGCAGCTT ATTCCACCTC CTTCATTCCC 
AATGCCACCC TGTCCAACTC CACGGATGTT ATCACCCTCA GCTCATCTAG TCATTTATCC 
TTATCAGCTG TCTCAGCTTT GGCCACTTCT ACCCCCACGC GTGCACCACA AGAGAACGGT 
GTGACGCCTT CAGGCCCCCC CAGTGAGTCG GGCCACTTGG ATGGAAGTGT TCATAGTCAC 
AGTACTTTGG CTCCCACTTC ACCATCATGT CCTCCAGAAG AACAGTGGAT CTATGACTCA 
CCCGAAAATG TGGTTCCACA CCGCACTCTG ACCTCCAGCT GTTCCACTCC CATCAACCAG 
CTTTATAGCA GCCTGGACCA TTCCTCACGG ACTGATTCCA GCTCCCTCTA CTCCCAGGAC 
AACGATGGAT ACTTCACCTC TATGCACATG GACTCAGGGC TGCGCTCCCG AAGTCACGGA 
AGTGGGCACG GCGCAGGAGC TGGGCGGTCC CGACACAGCA TGTATGAGTG TCGCGAGATG 
GCCAATCGAG AAGACTCTGG AAGCGTGTAC AGCGACCGCT CTCTGTCGCG CAGCATCTCG 
CTCCGCAAGT CAAAGAAGCC ACCGCCACCT CCGGCACGCA CAGATTCTCT GAGGCGCAAG 
CCTGCCGCAA AAAAGCCCTT AGGTGGTGTT GGTCCAACAA CTGTTGCCAA TGGGGACGGT 
GCTAACATGC TCAATGAAAC GTTCCTTGCT AGCTTGCAGC AGAGCCTGCA AATGGGACTG 
AGGGGGTCAA AGGGAAAGGG TACCTCTCTA TCTTCACCCT CTCACAGCCC CAGCAGTGAC 
TATGATGACC CTTGGTTGTT GCGACCGCGC AGTCAGAGTA GCATCAGTGC AGGCAGCTCT 
GCCGCCTCAT TAGCTAACAC AAATGGTGTG TCTAATGTCT ACTCATTATG CCATGTCACG 
CCCACGCATA GTGACACCAG CAGTTTGCGT TCTGACTATG CAGAGTCCTG GGGATACTAC 
ATGGACTACC CTCGTAACCC TGGAGACCAG GGGGGGCAGA CCCCACCAGC CCATGCTACA 
GATAACATGT CAGCTGGGGG TCATCCAGGA GTCCTACAGA ATGGAGGAGA GATTCACAGC 
AATACTCAAA CAGATGGAGC TCCAGGCCAG GAGGGAGGGC AGTCAGGGAA GCCCAAACCA 
TCCAGCTCCT CACCGGACAG GGTGCACAGA CTTACCTCCC CGTCAAGCGG TTACTCCAGC 
CAGTCCAACA CGCCTACAGC CGGAACCCCG GTGCCAGCCT TCGTCAGATC CATGTCTCCC 
TCCTCAGGGA GTCGGCCCAA ACCCAAAGTG CCGGAGAGGA AGTCCTCGCT CCTCTCTTCG 
GGTTCTGTCT CTTCGTCCTC TACTTCTTTG TCCTCCAACA CTTCAGACTC ACTAAAGAGT 
TCAGGTCCTC CTCTGCCACC CCCTAATCCC CTCCATCTCA CCTCTTCTAC CCCAAACACC 
CCTCTTGGTC CTCCTCCGCC CTTTCCTCCT CCTCTTCCTC CAAATTCTAC ATCAGCTCCC 
TCATCTCCTC CTCCACCTCC TCCATTACCC AACTCTCCAA GAGAAACCTT CTTGGCCCCT 
CTATCTGCGT CACCAGAATT CCCTCCTCCT CCATCTCCAG ACCTGTTGAT GCACCACAGT 
TCGTCCTTAA ATGGGAGCTT TAGTCCTCCA CCTCCCCCTG AGACCGGGAT CCCTCCACCA 
CCTCCACTGC CTGACTTCAC CCCAGCTTTT GGATCCCCAA ATATTAGGAA ATTGACAAAA 
AATACTCCTA AACCACATCC TTCCAAACAC TCCACAGAGT CCCCCAAGCC CCTCATCACC 
CCCTTCGCCC TGCAGAGTGT CCAGCTGCGC TCCGTCAAGC GATCACAAGA GGAGATCACT 
GGGGAGCTGG ACCAAATGAA ACCTCAGGAA AATGGACTGG ACCTTCTCCA GGAGCTGAAA 
CTCCATAGTC TAGATCCATC CCCCATCCAG GAGCACACCA CTGTCACGAG TAGCTCTCCA 
GACGAAGAGC TGCGGGAGTC CACACCTTCG CCTGTGACGA AACTGCTGGA AGAGCTGAGT 
CTGGACTGCA GTTTTATAAT CGACACACAG GATAGTGGTG TCATCAATGG AAAAGCTGAG 
GACCAGAGTT ACTGTCTATC AAATGGAAAA GACAATGAGG AAGAGCTGCC ACTGCCTAGC 
TGTCCAGAGA GATGTGGAAG CTCCTCCATC AAAGCCAAGC CTCCGCCAGT CTCCAAGAAA 
CCCAAACTTA CCTTTGTTCC ATCCCAACCA ATCAGCGAGC CAGCTCAAAC TCAAAATGAC 
AATGCAGCCA GTCTGTCGCC AGAGGATCAG GTCGATGCAC AAACGACAAA TGACGTAACA 
AATAAGAGGA ACAACCGGGA GGAAGAGGCG TCAGAGAGCA CAAAGGCTTC CACTGAGAAC 
CTGGACCAGA GCTGCCAAGA TGCAGATCTT GACCACGACA TTTGCACTAA TGGAGAGGTG 
TTTGATGAGG ATGAAGAAGA GGAAGAAGGA GAAGGAGCAA GCAGCACCAC GGGGTCCATC 
AGCTCAAAAG AGGAAGACAC AAGTGAAGTT TTCGAATCCA GACCGGCTGA ACCTCCTTCA 
CCCCAGTCGG CCGTAGGGGC AAGCGAGCAA AAAATGGTGA CCCCGACTCC CTCCAAACCC 
CGAACCACTG ATGACCTTTT TGCTGTCATA CACAGGTCAA AGCGAAAGGT TCTCGGTCGC 
AGAGAGTCGG ATGAAGACAA GTCCCGCCCC GGGGTTCAGG CACAGTCTCC ACCCTCCACC 
CCGTCACCCG TCTCTCCTGG GCCGGTTTCT CCGTCGGCCC GTCAGATGGG TTCCATCCAG 
CGAAACCTAC GCAAGTCCGC CACCAGCAGC GACAACTTCA AGGCCCTCCT GCTTCGGAAG 
GGAAGCCGCT CTGAGAACAG TTTCCGCATG TCTGCTACTG AAATGCTGCG CTCCACCGAC 
CCGCGCTTCC AGCGAACTCG CTCCGAGTCT GCGCTGGACC CCCCCACTGC GTCTCCCACG 
TCACCGATGG CGCCTCACAG CCCCTCTGCC TCTTTTGGGC GGAGTAAGAG GGTAACGGAT 
GAGTGGAGCC GCTACGATGC CCTGTCCTCG CCGACATATT CGCCTGGATC AAAGTTCGGG 
CGCTCTCGCG CGCCGCCTTC CGCCGCCAGC AGCAAATACA ACACTCGCAG CCGAATCCTC 
AGCAGCCCGA TGACTGTGAT CTGTGAGCGT GAAGGGGAGC TGACTGAGAG CGAATCTGGA 
GAGGTCGGCG AGAGCCGGGA AGGAGTCCAG ACGCTTCCAG AAACCAACGG CACTTTATCC 
GAAGAGAGCA GAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023809094.1
CDS	123..4640
Translation	MFCLKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSEVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_023953326.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023953326.1

ORF Insert Sequence:

ATGTTTTGTC TGAAAGCTGT GTCAAACCTG GATGAGGAGA GCAAATGGAC GGTACACTAC 
ACGGCTCCTT GGCACCAGCA GGAAAACGTC TTCCTGCCGG GCAGCAGGCC GTCCTGCGTG 
GAGGACCTCC ACCGCCAAGC CAAAGTCAAC CTCAAGACCA CCCTGCGAGA ATGTGATAAG 
TTGAGGAAAG ATGGTTTCCG GAGCTCCCAG TACTACTCTC AGGGCCCGAC CTTCTCCGAG 
CCCTTACAGT CCAGCAGCAG CCTGCAAGAC GAGGAAGATG AGGAAAATGA CAAGAAGTCT 
TTAGCATCCT CGATGGAGGA TGATAAATCC CAGCTCTCGC TGAGGTTGCA GGAGCGCTGC 
GGTGAAAGGG GCGAGATGTT GGAAGTTGAC GGGCAGATGG TGTGGAATAA AGCGGTTCCC 
CTCCCGACGC CGGAGGAGAG GATGAGGCAA GCAGCTCAGG CCGTGCCCAC GGACATAGTC 
CCCATCAATG TCACAGGGGC AGTGTTTGAC CGCCAGGCGA GCATCCGGCG CTCCCTCATT 
AACACTGATA CCGTGTCCCG TCGGCCCAAG AAGGTCAAAC GCAGAAAGAC TATATCAGGG 
CTGCCTGACA ACTTCAACCA GGAGCTAGCT AAAGGACGAG GCGGGGAACT TCGGCCACAT 
TCCATGTTCA TCCCAGGACA GTACTCCACT TTGGGGAGAG TTGGGAGTGT CAACTCAACA 
CTCCGCTTCG AGAAAAGAGA CTCCAGCTGC CAGACAGAAG AAGTGAAGAT TGTCCCTCCA 
TCTGTGAGAA GAATTCGAGC ACAAAGAGGG CAGGGAATCG CCGCTCAAAT GGCTGGGATA 
TCTGCTTCCT CTTCTACTGG AAGCATCTCC ATCTCGAGCA CAGATGGCTT GATTCTGCCC 
AATCAGTTTT TCAGAGACCC TTCCCGCTTT CACAGTCTGC CGCGACAGGG TGCCAGAGTG 
TCCCTCAGTG CTGATCCCAT CTATAGCAGC ACGCCCATTA AGTCAGAGGA TCAGTTGCAG 
AGGAATATTG GAAAATTCCA GGTTGACGAA ACAGCGGTAC ACATCAGGAA TGCCCCACGA 
ACGAGCACCT TGCCCAGACC CACGTCTCAG GAGGTGAGGA GGACGCAGTC CAGCGAGTGG 
GGAGGTGGTC CAGCATGTGT GGTCTCCCCT CAGGCAGCTT ATTCCACCTC CTTCATTCCC 
AATGCCACCC TGTCCAACTC CACGGATGTT ATCACCCTCA GCTCATCTAG TCATTTATCC 
TTATCAGCTG TCTCAGCTTT GGCCACTTCT ACCCCCACGC GTGCACCACA AGAGAACGGT 
GTGACGCCTT CAGGCCCCCC CAGTGAGTCG GGCCACTTGG ATGGAAGTGT TCATAGTCAC 
AGTACTTTGG CTCCCACTTC ACCATCATGT CCTCCAGAAG AACAGTGGAT CTATGACTCA 
CCCGAAAATG TGGTTCCACA CCGCACTCTG ACCTCCAGCT GTTCCACTCC CATCAACCAG 
CTTTATAGCA GCCTGGACCA TTCCTCACGG ACTGATTCCA GCTCCCTCTA CTCCCAGGAC 
AACGATGGAT ACTTCACCTC TATGCACATG GACTCAGGGC TGCGCTCCCG AAGTCACGGA 
AGTGGGCACG GCGCAGGAGC TGGGCGGTCC CGACACAGCA TGTATGAGTG TCGCGAGATG 
GCCAATCGAG AAGACTCTGG AAGCGTGTAC AGCGACCGCT CTCTGTCGCG CAGCATCTCG 
CTCCGCAAGT CAAAGAAGCC ACCGCCACCT CCGGCACGCA CAGATTCTCT GAGGCGCAAG 
CCTGCCGCAA AAAAGCCCTT AGGTGGTGTT GGTCCAACAA CTGTTGCCAA TGGGGACGGT 
GCTAACATGC TCAATGAAAC GTTCCTTGCT AGCTTGCAGC AGAGCCTGCA AATGGGACTG 
AGGGGGTCAA AGGGAAAGGG TACCTCTCTA TCTTCACCCT CTCACAGCCC CAGCAGTGAC 
TATGATGACC CTTGGTTGTT GCGACCGCGC AGTCAGAGTA GCATCAGTGC AGGCAGCTCT 
GCCGCCTCAT TAGCTAACAC AAATGGTGTG TCTAATGTCT ACTCATTATG CCATGTCACG 
CCCACGCATA GTGACACCAG CAGTTTGCGT TCTGACTATG CAGAGTCCTG GGGATACTAC 
ATGGACTACC CTCGTAACCC TGGAGACCAG GGGGGGCAGA CCCCACCAGC CCATGCTACA 
GATAACATGT CAGCTGGGGG TCATCCAGGA GTCCTACAGA ATGGAGGAGA GATTCACAGC 
AATACTCAAA CAGATGGAGC TCCAGGCCAG GAGGGAGGGC AGTCAGGGAA GCCCAAACCA 
TCCAGCTCCT CACCGGACAG GGTGCACAGA CTTACCTCCC CGTCAAGCGG TTACTCCAGC 
CAGTCCAACA CGCCTACAGC CGGAACCCCG GTGCCAGCCT TCGTCAGATC CATGTCTCCC 
TCCTCAGGGA GTCGGCCCAA ACCCAAAGTG CCGGAGAGGA AGTCCTCGCT CCTCTCTTCG 
GGTTCTGTCT CTTCGTCCTC TACTTCTTTG TCCTCCAACA CTTCAGACTC ACTAAAGAGT 
TCAGGTCCTC CTCTGCCACC CCCTAATCCC CTCCATCTCA CCTCTTCTAC CCCAAACACC 
CCTCTTGGTC CTCCTCCGCC CTTTCCTCCT CCTCTTCCTC CAAATTCTAC ATCAGCTCCC 
TCATCTCCTC CTCCACCTCC TCCATTACCC AACTCTCCAA GAGAAACCTT CTTGGCCCCT 
CTATCTGCGT CACCAGAATT CCCTCCTCCT CCATCTCCAG ACCTGTTGAT GCACCACAGT 
TCGTCCTTAA ATGGGAGCTT TAGTCCTCCA CCTCCCCCTG AGACCGGGAT CCCTCCACCA 
CCTCCACTGC CTGACTTCAC CCCAGCTTTT GGATCCCCAA ATATTAGGAA ATTGACAAAA 
AATACTCCTA AACCACATCC TTCCAAACAC TCCACAGAGT CCCCCAAGCC CCTCATCACC 
CCCTTCGCCC TGCAGAGTGT CCAGCTGCGC TCCGTCAAGC GATCACAAGA GGAGATCACT 
GGGGAGCTGG ACCAAATGAA ACCTCAGGAA AATGGACTGG ACCTTCTCCA GGAGCTGAAA 
CTCCATAGTC TAGATCCATC CCCCATCCAG GAGCACACCA CTGTCACGAG TAGCTCTCCA 
GACGAAGAGC TGCGGGAGTC CACACCTTCG CCTGTGACGA AACTGCTGGA AGAGCTGAGT 
CTGGACTGCA GTTTTATAAT CGACACACAG GATAGTGGTG TCATCAATGG AAAAGCTGAG 
GACCAGAGTT ACTGTCTATC AAATGGAAAA GACAATGAGG AAGAGCTGCC ACTGCCTAGC 
TGTCCAGAGA GATGTGGAAG CTCCTCCATC AAAGCCAAGC CTCCGCCAGT CTCCAAGAAA 
CCCAAACTTA CCTTTGTTCC ATCCCAACCA ATCAGCGAGC CAGCTCAAAC TCAAAATGAC 
AATGCAGCCA GTCTGTCGCC AGAGGATCAG GTCGATGCAC AAACGACAAA TGACGTAACA 
AATAAGAGGA ACAACCGGGA GGAAGAGGCG TCAGAGAGCA CAAAGGCTTC CACTGAGAAC 
CTGGACCAGA GCTGCCAAGA TGCAGATCTT GACCACGACA TTTGCACTAA TGGAGAGGTG 
TTTGATGAGG ATGAAGAAGA GGAAGAAGGA GAAGGAGCAA GCAGCACCAC GGGGTCCATC 
AGCTCAAAAG AGGAAGACAC AAGTGAAGTT TTCGAATCCA GACCGGCTGA ACCTCCTTCA 
CCCCAGTCGG CCGTAGGGGC AAGCGAGCAA AAAATGGTGA CCCCGACTCC CTCCAAACCC 
CGAACCACTG ATGACCTTTT TGCTGTCATA CACAGGTCAA AGCGAAAGGT TCTCGGTCGC 
AGAGAGTCGG ATGAAGACAA GTCCCGCCCC GGGGTTCAGG CACAGTCTCC ACCCTCCACC 
CCGTCACCCG TCTCTCCTGG GCCGGTTTCT CCGTCGGCCC GTCAGATGGG TTCCATCCAG 
CGAAACCTAC GCAAGTCCGC CACCAGCAGC GACAACTTCA AGGCCCTCCT GCTTCGGAAG 
GGAAGCCGCT CTGAGAACAG TTTCCGCATG TCTGCTACTG AAATGCTGCG CTCCACCGAC 
CCGCGCTTCC AGCGAACTCG CTCCGAGTCT GCGCTGGACC CCCCCACTGC GTCTCCCACG 
TCACCGATGG CGCCTCACAG CCCCTCTGCC TCTTTTGGGC GGAGTAAGAG GGTAACGGAT 
GAGTGGAGCC GCTACGATGC CCTGTCCTCG CCGACATATT CGCCTGGATC AAAGTTCGGG 
CGCTCTCGCG CGCCGCCTTC CGCCGCCAGC AGCAAATACA ACACTCGCAG CCGAATCCTC 
AGCAGCCCGA TGACTGTGAT CTGTGAGCGT GAAGGGGAGC TGACTGAGAG CGAATCTGGA 
GAGGTCGGCG AGAGCCGGGA AGGAGTCCAG ACGCTTCCAG AAACCAACGG CACTTTATCC 
GAAGAGAGCA GAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714401.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570060.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.

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CloneID	OJa76379
Clone ID Related Accession (Same CDS sequence)	XM_020714401.1 , XM_020714402.1
Accession Version	XM_020714401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GANGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570060.1
CDS	344..5167
Translation	MPFHQRTVEPRRVSRLSARYGWKAREEEPGKRKTRRLVLFSSLDEVSCHTLTGLIHQLSDLSRHASDIFLGIEVEAGAVFRRSCRIQGRLQFLQDEVCRLDPKKVKIPVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714401.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714401.1

ORF Insert Sequence:

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GANGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714402.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570061.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.

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CloneID	OJa76379
Clone ID Related Accession (Same CDS sequence)	XM_020714401.1 , XM_020714402.1
Accession Version	XM_020714402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GANGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570061.1
CDS	241..5064
Translation	MPFHQRTVEPRRVSRLSARYGWKAREEEPGKRKTRRLVLFSSLDEVSCHTLTGLIHQLSDLSRHASDIFLGIEVEAGAVFRRSCRIQGRLQFLQDEVCRLDPKKVKIPVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714402.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714402.1

ORF Insert Sequence:

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGGGGCAGTG TTTGACCGCC AGGCGAGCAT CCGGCGCTCC 
CTCATTAACA CTGATACCGT GTCCCGTCGG CCCAAGAAGG TCAAACGCAG AAAGACTATA 
TCAGGGCTGC CTGACAACTT CAACCAGGAG CTAGCTAAAG GACGAGGCGG GGAACTTCGG 
CCACATTCCA TGTTCATCCC AGGACAGTAC TCCACTTTGG GGAGAGTTGG GAGTGTCAAC 
TCAACACTCC GCTTCGAGAA AAGAGACTCC AGCTGCCAGA CAGAAGAAGT GAAGATTGTC 
CCTCCATCTG TGAGAAGAAT TCGAGCACAA AGAGGGCAGG GAATCGCCGC TCAAATGGCT 
GGGATATCTG CTTCCTCTTC TACTGGAAGC ATCTCCATCT CGAGCACAGA TGGCTTGATT 
CTGCCCAATC AGTTTTTCAG AGACCCTTCC CGCTTTCACA GTCTGCCGCG ACAGGGTGCC 
AGAGTGTCCC TCAGTGCTGA TCCCATCTAT AGCAGCACGC CCATTAAGTC AGAGGATCAG 
TTGCAGAGGA ATATTGGAAA ATTCCAGGTT GACGAAACAG CGGTACACAT CAGGAATGCC 
CCACGAACGA GCACCTTGCC CAGACCCACG TCTCAGGAGG TGAGGAGGAC GCAGTCCAGC 
GAGTGGGGAG GTGGTCCAGC ATGTGTGGTC TCCCCTCAGG CAGCTTATTC CACCTCCTTC 
ATTCCCAATG CCACCCTGTC CAACTCCACG GATGTTATCA CCCTCAGCTC ATCTAGTCAT 
TTATCCTTAT CAGCTGTCTC AGCTTTGGCC ACTTCTACCC CCACGCGTGC ACCACAAGAG 
AACGGTGTGA CGCCTTCAGG CCCCCCCAGT GAGTCGGGCC ACTTGGATGG AAGTGTTCAT 
AGTCACAGTA CTTTGGCTCC CACTTCACCA TCATGTCCTC CAGAAGAACA GTGGATCTAT 
GACTCACCCG AAAATGTGGT TCCACACCGC ACTCTGACCT CCAGCTGTTC CACTCCCATC 
AACCAGCTTT ATAGCAGCCT GGACCATTCC TCACGGACTG ATTCCAGCTC CCTCTACTCC 
CAGGACAACG ATGGATACTT CACCTCTATG CACATGGACT CAGGGCTGCG CTCCCGAAGT 
CACGGAAGTG GGCACGGCGC AGGAGCTGGG CGGTCCCGAC ACAGCATGTA TGAGTGTCGC 
GAGATGGCCA ATCGAGAAGA CTCTGGAAGC GTGTACAGCG ACCGCTCTCT GTCGCGCAGC 
ATCTCGCTCC GCAAGTCAAA GAAGCCACCG CCACCTCCGG CACGCACAGA TTCTCTGAGG 
CGCAAGCCTG CCGCAAAAAA GCCCTTAGGT GGTGTTGGTC CAACAACTGT TGCCAATGGG 
GACGGTGCTA ACATGCTCAA TGAAACGTTC CTTGCTAGCT TGCAGCAGAG CCTGCAAATG 
GGACTGAGGG GGTCAAAGGG AAAGGGTACC TCTCTATCTT CACCCTCTCA CAGCCCCAGC 
AGTGACTATG ATGACCCTTG GTTGTTGCGA CCGCGCAGTC AGAGTAGCAT CAGTGCAGGC 
AGCTCTGCCG CCTCATTAGC TAACACAAAT GGTGTGTCTA ATGTCTACTC ATTATGCCAT 
GTCACGCCCA CGCATAGTGA CACCAGCAGT TTGCGTTCTG ACTATGCAGA GTCCTGGGGA 
TACTACATGG ACTACCCTCG TAACCCTGGA GACCAGGGGG GGCAGACCCC ACCAGCCCAT 
GCTACAGATA ACATGTCAGC TGGGGGTCAT CCAGGAGTCC TACAGAATGG AGGAGAGATT 
CACAGCAATA CTCAAACAGA TGGAGCTCCA GGCCAGGAGG GAGGGCAGTC AGGGAAGCCC 
AAACCATCCA GCTCCTCACC GGACAGGGTG CACAGACTTA CCTCCCCGTC AAGCGGTTAC 
TCCAGCCAGT CCAACACGCC TACAGCCGGA ACCCCGGTGC CAGCCTTCGT CAGATCCATG 
TCTCCCTCCT CAGGGAGTCG GCCCAAACCC AAAGTGCCGG AGAGGAAGTC CTCGCTCCTC 
TCTTCGGGTT CTGTCTCTTC GTCCTCTACT TCTTTGTCCT CCAACACTTC AGACTCACTA 
AAGAGTTCAG GTCCTCCTCT GCCACCCCCT AATCCCCTCC ATCTCACCTC TTCTACCCCA 
AACACCCCTC TTGGTCCTCC TCCGCCCTTT CCTCCTCCTC TTCCTCCAAA TTCTACATCA 
GCTCCCTCAT CTCCTCCTCC ACCTCCTCCA TTACCCAACT CTCCAAGAGA AACCTTCTTG 
GCCCCTCTAT CTGCGTCACC AGAATTCCCT CCTCCTCCAT CTCCAGACCT GTTGATGCAC 
CACAGTTCGT CCTTAAATGG GAGCTTTAGT CCTCCACCTC CCCCTGAGAC CGGGATCCCT 
CCACCACCTC CACTGCCTGA CTTCACCCCA GCTTTTGGAT CCCCAAATAT TAGGAAATTG 
ACAAAAAATA CTCCTAAACC ACATCCTTCC AAACACTCCA CAGAGTCCCC CAAGCCCCTC 
ATCACCCCCT TCGCCCTGCA GAGTGTCCAG CTGCGCTCCG TCAAGCGATC ACAAGAGGAG 
ATCACTGGGG AGCTGGACCA AATGAAACCT CAGGAAAATG GACTGGACCT TCTCCAGGAG 
CTGAAACTCC ATAGTCTAGA TCCATCCCCC ATCCAGGAGC ACACCACTGT CACGAGTAGC 
TCTCCAGACG AAGAGCTGCG GGAGTCCACA CCTTCGCCTG TGACGAAACT GCTGGAAGAG 
CTGAGTCTGG ACTGCAGTTT TATAATCGAC ACACAGGATA GTGGTGTCAT CAATGGAAAA 
GCTGAGGACC AGAGTTACTG TCTATCAAAT GGAAAAGACA ATGAGGAAGA GCTGCCACTG 
CCTAGCTGTC CAGAGAGATG TGGAAGCTCC TCCATCAAAG CCAAGCCTCC GCCAGTCTCC 
AAGAAACCCA AACTTACCTT TGTTCCATCC CAACCAATCA GCGAGCCAGC TCAAACTCAA 
AATGACAATG CAGCCAGTCT GTCGCCAGAG GATCAGGTCG ATGCACAAAC GACAAATGAC 
GTAACAAATA AGAGGAACAA CCGGGAGGAA GAGGCGTCAG AGAGCACAAA GGCTTCCACT 
GAGAACCTGG ACCAGAGCTG CCAAGATGCA GATCTTGACC ACGACATTTG CACTAATGGA 
GAGGTGTTTG ATGAGGATGA AGAAGAGGAA GAAGGAGAAG GAGCAAGCAG CACCACGGGG 
TCCATCAGCT CAAAAGAGGA AGACACAAGT GANGTTTTCG AATCCAGACC GGCTGAACCT 
CCTTCACCCC AGTCGGCCGT AGGGGCAAGC GAGCAAAAAA TGGTGACCCC GACTCCCTCC 
AAACCCCGAA CCACTGATGA CCTTTTTGCT GTCATACACA GGTCAAAGCG AAAGGTTCTC 
GGTCGCAGAG AGTCGGATGA AGACAAGTCC CGCCCCGGGG TTCAGGCACA GTCTCCACCC 
TCCACCCCGT CACCCGTCTC TCCTGGGCCG GTTTCTCCGT CGGCCCGTCA GATGGGTTCC 
ATCCAGCGAA ACCTACGCAA GTCCGCCACC AGCAGCGACA ACTTCAAGGC CCTCCTGCTT 
CGGAAGGGAA GCCGCTCTGA GAACAGTTTC CGCATGTCTG CTACTGAAAT GCTGCGCTCC 
ACCGACCCGC GCTTCCAGCG AACTCGCTCC GAGTCTGCGC TGGACCCCCC CACTGCGTCT 
CCCACGTCAC CGATGGCGCC TCACAGCCCC TCTGCCTCTT TTGGGCGGAG TAAGAGGGTA 
ACGGATGAGT GGAGCCGCTA CGATGCCCTG TCCTCGCCGA CATATTCGCC TGGATCAAAG 
TTCGGGCGCT CTCGCGCGCC GCCTTCCGCC GCCAGCAGCA AATACAACAC TCGCAGCCGA 
ATCCTCAGCA GCCCGATGAC TGTGATCTGT GAGCGTGAAG GGGAGCTGAC TGAGAGCGAA 
TCTGGAGAGG TCGGCGAGAG CCGGGAAGGA GTCCAGACGC TTCCAGAAAC CAACGGCACT 
TTATCCGAAG AGAGCAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714403.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570062.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa76380
Clone ID Related Accession (Same CDS sequence)	XM_020714403.1
Accession Version	XM_020714403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACAT ACATGCACTG CCTCTCCTCG GAGCCGGCGG TCAGTTGCTG GCACGGCGCC 
TGCTATGAGG ACCCCTCTGG CCAGAACAAA ACTCTTTGCT CCTTCAAGCC CAGATGCGAG 
ATGGGGAACA AACTCCAGGT TCAGGCCCTC CCCAGTTCCA ACCCGTGCAG ACCGGAGGGG 
GGCCTGCGCA GGCGGCTGCT CTCTGTGTCG AAAGTGCACC AGAAGCCGGA CTCGCTCTGG 
ACTCCCAAAC CTCTGCTGGG ACCCGCGGCC GATGACTTAC AGAGGAAAAC ACAAAGTTGT 
CAGGATGGGA AAGCTGTGTC AAACCTGGAT GAGGAGAGCA AATGGACGGT ACACTACACG 
GCTCCTTGGC ACCAGCAGGA AAACGTCTTC CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG 
GACCTCCACC GCCAAGCCAA AGTCAACCTC AAGACCACCC TGCGAGAATG TGATAAGTTG 
AGGAAAGATG GTTTCCGGAG CTCCCAGTAC TACTCTCAGG GCCCGACCTT CTCCGAGCCC 
TTACAGTCCA GCAGCAGCCT GCAAGACGAG GAAGATGAGG AAAATGACAA GAAGTCTTTA 
GCATCCTCGA TGGAGGATGA TAAATCCCAG CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT 
GAAAGGGGCG AGATGTTGGA AGTTGACGGG CAGATGGTGT GGAATAAAGC GGTTCCCCTC 
CCGACGCCGG AGGAGAGGAT GAGGCAAGCA GCTCAGGCCG TGCCCACGGA CATAGTCCCC 
ATCAATGTCA CAGGGGCAGT GTTTGACCGC CAGGCGAGCA TCCGGCGCTC CCTCATTAAC 
ACTGATACCG TGTCCCGTCG GCCCAAGAAG GTCAAACGCA GAAAGACTAT ATCAGGGCTG 
CCTGACAACT TCAACCAGGA GCTAGCTAAA GGACGAGGCG GGGAACTTCG GCCACATTCC 
ATGTTCATCC CAGGACAGTA CTCCACTTTG GGGAGAGTTG GGAGTGTCAA CTCAACACTC 
CGCTTCGAGA AAAGAGACTC CAGCTGCCAG ACAGAAGAAG TGAAGATTGT CCCTCCATCT 
GTGAGAAGAA TTCGAGCACA AAGAGGGCAG GGAATCGCCG CTCAAATGGC TGGGATATCT 
GCTTCCTCTT CTACTGGAAG CATCTCCATC TCGAGCACAG ATGGCTTGAT TCTGCCCAAT 
CAGTTTTTCA GAGACCCTTC CCGCTTTCAC AGTCTGCCGC GACAGGGTGC CAGAGTGTCC 
CTCAGTGCTG ATCCCATCTA TAGCAGCACG CCCATTAAGT CAGAGGATCA GTTGCAGAGG 
AATATTGGAA AATTCCAGGT TGACGAAACA GCGGTACACA TCAGGAATGC CCCACGAACG 
AGCACCTTGC CCAGACCCAC GTCTCAGGAG GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA 
GGTGGTCCAG CATGTGTGGT CTCCCCTCAG GCAGCTTATT CCACCTCCTT CATTCCCAAT 
GCCACCCTGT CCAACTCCAC GGATGTTATC ACCCTCAGCT CATCTAGTCA TTTATCCTTA 
TCAGCTGTCT CAGCTTTGGC CACTTCTACC CCCACGCGTG CACCACAAGA GAACGGTGTG 
ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC CACTTGGATG GAAGTGTTCA TAGTCACAGT 
ACTTTGGCTC CCACTTCACC ATCATGTCCT CCAGAAGAAC AGTGGATCTA TGACTCACCC 
GAAAATGTGG TTCCACACCG CACTCTGACC TCCAGCTGTT CCACTCCCAT CAACCAGCTT 
TATAGCAGCC TGGACCATTC CTCACGGACT GATTCCAGCT CCCTCTACTC CCAGGACAAC 
GATGGATACT TCACCTCTAT GCACATGGAC TCAGGGCTGC GCTCCCGAAG TCACGGAAGT 
GGGCACGGCG CAGGAGCTGG GCGGTCCCGA CACAGCATGT ATGAGTGTCG CGAGATGGCC 
AATCGAGAAG ACTCTGGAAG CGTGTACAGC GACCGCTCTC TGTCGCGCAG CATCTCGCTC 
CGCAAGTCAA AGAAGCCACC GCCACCTCCG GCACGCACAG ATTCTCTGAG GCGCAAGCCT 
GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT CCAACAACTG TTGCCAATGG GGACGGTGCT 
AACATGCTCA ATGAAACGTT CCTTGCTAGC TTGCAGCAGA GCCTGCAAAT GGGACTGAGG 
GGGTCAAAGG GAAAGGGTAC CTCTCTATCT TCACCCTCTC ACAGCCCCAG CAGTGACTAT 
GATGACCCTT GGTTGTTGCG ACCGCGCAGT CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC 
GCCTCATTAG CTAACACAAA TGGTGTGTCT AATGTCTACT CATTATGCCA TGTCACGCCC 
ACGCATAGTG ACACCAGCAG TTTGCGTTCT GACTATGCAG AGTCCTGGGG ATACTACATG 
GACTACCCTC GTAACCCTGG AGACCAGGGG GGGCAGACCC CACCAGCCCA TGCTACAGAT 
AACATGTCAG CTGGGGGTCA TCCAGGAGTC CTACAGAATG GAGGAGAGAT TCACAGCAAT 
ACTCAAACAG ATGGAGCTCC AGGCCAGGAG GGAGGGCAGT CAGGGAAGCC CAAACCATCC 
AGCTCCTCAC CGGACAGGGT GCACAGACTT ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG 
TCCAACACGC CTACAGCCGG AACCCCGGTG CCAGCCTTCG TCAGATCCAT GTCTCCCTCC 
TCAGGGAGTC GGCCCAAACC CAAAGTGCCG GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT 
TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC TCCAACACTT CAGACTCACT AAAGAGTTCA 
GGTCCTCCTC TGCCACCCCC TAATCCCCTC CATCTCACCT CTTCTACCCC AAACACCCCT 
CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT CTTCCTCCAA ATTCTACATC AGCTCCCTCA 
TCTCCTCCTC CACCTCCTCC ATTACCCAAC TCTCCAAGAG AAACCTTCTT GGCCCCTCTA 
TCTGCGTCAC CAGAATTCCC TCCTCCTCCA TCTCCAGACC TGTTGATGCA CCACAGTTCG 
TCCTTAAATG GGAGCTTTAG TCCTCCACCT CCCCCTGAGA CCGGGATCCC TCCACCACCT 
CCACTGCCTG ACTTCACCCC AGCTTTTGGA TCCCCAAATA TTAGGAAATT GACAAAAAAT 
ACTCCTAAAC CACATCCTTC CAAACACTCC ACAGAGTCCC CCAAGCCCCT CATCACCCCC 
TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC GTCAAGCGAT CACAAGAGGA GATCACTGGG 
GAGCTGGACC AAATGAAACC TCAGGAAAAT GGACTGGACC TTCTCCAGGA GCTGAAACTC 
CATAGTCTAG ATCCATCCCC CATCCAGGAG CACACCACTG TCACGAGTAG CTCTCCAGAC 
GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT GTGACGAAAC TGCTGGAAGA GCTGAGTCTG 
GACTGCAGTT TTATAATCGA CACACAGGAT AGTGGTGTCA TCAATGGAAA AGCTGAGGAC 
CAGAGTTACT GTCTATCAAA TGGAAAAGAC AATGAGGAAG AGCTGCCACT GCCTAGCTGT 
CCAGAGAGAT GTGGAAGCTC CTCCATCAAA GCCAAGCCTC CGCCAGTCTC CAAGAAACCC 
AAACTTACCT TTGTTCCATC CCAACCAATC AGCGAGCCAG CTCAAACTCA AAATGACAAT 
GCAGCCAGTC TGTCGCCAGA GGATCAGGTC GATGCACAAA CGACAAATGA CGTAACAAAT 
AAGAGGAACA ACCGGGAGGA AGAGGCGTCA GAGAGCACAA AGGCTTCCAC TGAGAACCTG 
GACCAGAGCT GCCAAGATGC AGATCTTGAC CACGACATTT GCACTAATGG AGAGGTGTTT 
GATGAGGATG AAGAAGAGGA AGAAGGAGAA GGAGCAAGCA GCACCACGGG GTCCATCAGC 
TCAAAAGAGG AAGACACAAG TGANGTTTTC GAATCCAGAC CGGCTGAACC TCCTTCACCC 
CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA ATGGTGACCC CGACTCCCTC CAAACCCCGA 
ACCACTGATG ACCTTTTTGC TGTCATACAC AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA 
GAGTCGGATG AAGACAAGTC CCGCCCCGGG GTTCAGGCAC AGTCTCCACC CTCCACCCCG 
TCACCCGTCT CTCCTGGGCC GGTTTCTCCG TCGGCCCGTC AGATGGGTTC CATCCAGCGA 
AACCTACGCA AGTCCGCCAC CAGCAGCGAC AACTTCAAGG CCCTCCTGCT TCGGAAGGGA 
AGCCGCTCTG AGAACAGTTT CCGCATGTCT GCTACTGAAA TGCTGCGCTC CACCGACCCG 
CGCTTCCAGC GAACTCGCTC CGAGTCTGCG CTGGACCCCC CCACTGCGTC TCCCACGTCA 
CCGATGGCGC CTCACAGCCC CTCTGCCTCT TTTGGGCGGA GTAAGAGGGT AACGGATGAG 
TGGAGCCGCT ACGATGCCCT GTCCTCGCCG ACATATTCGC CTGGATCAAA GTTCGGGCGC 
TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC AAATACAACA CTCGCAGCCG AATCCTCAGC 
AGCCCGATGA CTGTGATCTG TGAGCGTGAA GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG 
GTCGGCGAGA GCCGGGAAGG AGTCCAGACG CTTCCAGAAA CCAACGGCAC TTTATCCGAA 
GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570062.1
CDS	39..4853
Translation	MITYMHCLSSEPAVSCWHGACYEDPSGQNKTLCSFKPRCEMGNKLQVQALPSSNPCRPEGGLRRRLLSVSKVHQKPDSLWTPKPLLGPAADDLQRKTQSCQDGKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714403.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714403.1

ORF Insert Sequence:

ATGATCACAT ACATGCACTG CCTCTCCTCG GAGCCGGCGG TCAGTTGCTG GCACGGCGCC 
TGCTATGAGG ACCCCTCTGG CCAGAACAAA ACTCTTTGCT CCTTCAAGCC CAGATGCGAG 
ATGGGGAACA AACTCCAGGT TCAGGCCCTC CCCAGTTCCA ACCCGTGCAG ACCGGAGGGG 
GGCCTGCGCA GGCGGCTGCT CTCTGTGTCG AAAGTGCACC AGAAGCCGGA CTCGCTCTGG 
ACTCCCAAAC CTCTGCTGGG ACCCGCGGCC GATGACTTAC AGAGGAAAAC ACAAAGTTGT 
CAGGATGGGA AAGCTGTGTC AAACCTGGAT GAGGAGAGCA AATGGACGGT ACACTACACG 
GCTCCTTGGC ACCAGCAGGA AAACGTCTTC CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG 
GACCTCCACC GCCAAGCCAA AGTCAACCTC AAGACCACCC TGCGAGAATG TGATAAGTTG 
AGGAAAGATG GTTTCCGGAG CTCCCAGTAC TACTCTCAGG GCCCGACCTT CTCCGAGCCC 
TTACAGTCCA GCAGCAGCCT GCAAGACGAG GAAGATGAGG AAAATGACAA GAAGTCTTTA 
GCATCCTCGA TGGAGGATGA TAAATCCCAG CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT 
GAAAGGGGCG AGATGTTGGA AGTTGACGGG CAGATGGTGT GGAATAAAGC GGTTCCCCTC 
CCGACGCCGG AGGAGAGGAT GAGGCAAGCA GCTCAGGCCG TGCCCACGGA CATAGTCCCC 
ATCAATGTCA CAGGGGCAGT GTTTGACCGC CAGGCGAGCA TCCGGCGCTC CCTCATTAAC 
ACTGATACCG TGTCCCGTCG GCCCAAGAAG GTCAAACGCA GAAAGACTAT ATCAGGGCTG 
CCTGACAACT TCAACCAGGA GCTAGCTAAA GGACGAGGCG GGGAACTTCG GCCACATTCC 
ATGTTCATCC CAGGACAGTA CTCCACTTTG GGGAGAGTTG GGAGTGTCAA CTCAACACTC 
CGCTTCGAGA AAAGAGACTC CAGCTGCCAG ACAGAAGAAG TGAAGATTGT CCCTCCATCT 
GTGAGAAGAA TTCGAGCACA AAGAGGGCAG GGAATCGCCG CTCAAATGGC TGGGATATCT 
GCTTCCTCTT CTACTGGAAG CATCTCCATC TCGAGCACAG ATGGCTTGAT TCTGCCCAAT 
CAGTTTTTCA GAGACCCTTC CCGCTTTCAC AGTCTGCCGC GACAGGGTGC CAGAGTGTCC 
CTCAGTGCTG ATCCCATCTA TAGCAGCACG CCCATTAAGT CAGAGGATCA GTTGCAGAGG 
AATATTGGAA AATTCCAGGT TGACGAAACA GCGGTACACA TCAGGAATGC CCCACGAACG 
AGCACCTTGC CCAGACCCAC GTCTCAGGAG GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA 
GGTGGTCCAG CATGTGTGGT CTCCCCTCAG GCAGCTTATT CCACCTCCTT CATTCCCAAT 
GCCACCCTGT CCAACTCCAC GGATGTTATC ACCCTCAGCT CATCTAGTCA TTTATCCTTA 
TCAGCTGTCT CAGCTTTGGC CACTTCTACC CCCACGCGTG CACCACAAGA GAACGGTGTG 
ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC CACTTGGATG GAAGTGTTCA TAGTCACAGT 
ACTTTGGCTC CCACTTCACC ATCATGTCCT CCAGAAGAAC AGTGGATCTA TGACTCACCC 
GAAAATGTGG TTCCACACCG CACTCTGACC TCCAGCTGTT CCACTCCCAT CAACCAGCTT 
TATAGCAGCC TGGACCATTC CTCACGGACT GATTCCAGCT CCCTCTACTC CCAGGACAAC 
GATGGATACT TCACCTCTAT GCACATGGAC TCAGGGCTGC GCTCCCGAAG TCACGGAAGT 
GGGCACGGCG CAGGAGCTGG GCGGTCCCGA CACAGCATGT ATGAGTGTCG CGAGATGGCC 
AATCGAGAAG ACTCTGGAAG CGTGTACAGC GACCGCTCTC TGTCGCGCAG CATCTCGCTC 
CGCAAGTCAA AGAAGCCACC GCCACCTCCG GCACGCACAG ATTCTCTGAG GCGCAAGCCT 
GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT CCAACAACTG TTGCCAATGG GGACGGTGCT 
AACATGCTCA ATGAAACGTT CCTTGCTAGC TTGCAGCAGA GCCTGCAAAT GGGACTGAGG 
GGGTCAAAGG GAAAGGGTAC CTCTCTATCT TCACCCTCTC ACAGCCCCAG CAGTGACTAT 
GATGACCCTT GGTTGTTGCG ACCGCGCAGT CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC 
GCCTCATTAG CTAACACAAA TGGTGTGTCT AATGTCTACT CATTATGCCA TGTCACGCCC 
ACGCATAGTG ACACCAGCAG TTTGCGTTCT GACTATGCAG AGTCCTGGGG ATACTACATG 
GACTACCCTC GTAACCCTGG AGACCAGGGG GGGCAGACCC CACCAGCCCA TGCTACAGAT 
AACATGTCAG CTGGGGGTCA TCCAGGAGTC CTACAGAATG GAGGAGAGAT TCACAGCAAT 
ACTCAAACAG ATGGAGCTCC AGGCCAGGAG GGAGGGCAGT CAGGGAAGCC CAAACCATCC 
AGCTCCTCAC CGGACAGGGT GCACAGACTT ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG 
TCCAACACGC CTACAGCCGG AACCCCGGTG CCAGCCTTCG TCAGATCCAT GTCTCCCTCC 
TCAGGGAGTC GGCCCAAACC CAAAGTGCCG GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT 
TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC TCCAACACTT CAGACTCACT AAAGAGTTCA 
GGTCCTCCTC TGCCACCCCC TAATCCCCTC CATCTCACCT CTTCTACCCC AAACACCCCT 
CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT CTTCCTCCAA ATTCTACATC AGCTCCCTCA 
TCTCCTCCTC CACCTCCTCC ATTACCCAAC TCTCCAAGAG AAACCTTCTT GGCCCCTCTA 
TCTGCGTCAC CAGAATTCCC TCCTCCTCCA TCTCCAGACC TGTTGATGCA CCACAGTTCG 
TCCTTAAATG GGAGCTTTAG TCCTCCACCT CCCCCTGAGA CCGGGATCCC TCCACCACCT 
CCACTGCCTG ACTTCACCCC AGCTTTTGGA TCCCCAAATA TTAGGAAATT GACAAAAAAT 
ACTCCTAAAC CACATCCTTC CAAACACTCC ACAGAGTCCC CCAAGCCCCT CATCACCCCC 
TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC GTCAAGCGAT CACAAGAGGA GATCACTGGG 
GAGCTGGACC AAATGAAACC TCAGGAAAAT GGACTGGACC TTCTCCAGGA GCTGAAACTC 
CATAGTCTAG ATCCATCCCC CATCCAGGAG CACACCACTG TCACGAGTAG CTCTCCAGAC 
GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT GTGACGAAAC TGCTGGAAGA GCTGAGTCTG 
GACTGCAGTT TTATAATCGA CACACAGGAT AGTGGTGTCA TCAATGGAAA AGCTGAGGAC 
CAGAGTTACT GTCTATCAAA TGGAAAAGAC AATGAGGAAG AGCTGCCACT GCCTAGCTGT 
CCAGAGAGAT GTGGAAGCTC CTCCATCAAA GCCAAGCCTC CGCCAGTCTC CAAGAAACCC 
AAACTTACCT TTGTTCCATC CCAACCAATC AGCGAGCCAG CTCAAACTCA AAATGACAAT 
GCAGCCAGTC TGTCGCCAGA GGATCAGGTC GATGCACAAA CGACAAATGA CGTAACAAAT 
AAGAGGAACA ACCGGGAGGA AGAGGCGTCA GAGAGCACAA AGGCTTCCAC TGAGAACCTG 
GACCAGAGCT GCCAAGATGC AGATCTTGAC CACGACATTT GCACTAATGG AGAGGTGTTT 
GATGAGGATG AAGAAGAGGA AGAAGGAGAA GGAGCAAGCA GCACCACGGG GTCCATCAGC 
TCAAAAGAGG AAGACACAAG TGANGTTTTC GAATCCAGAC CGGCTGAACC TCCTTCACCC 
CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA ATGGTGACCC CGACTCCCTC CAAACCCCGA 
ACCACTGATG ACCTTTTTGC TGTCATACAC AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA 
GAGTCGGATG AAGACAAGTC CCGCCCCGGG GTTCAGGCAC AGTCTCCACC CTCCACCCCG 
TCACCCGTCT CTCCTGGGCC GGTTTCTCCG TCGGCCCGTC AGATGGGTTC CATCCAGCGA 
AACCTACGCA AGTCCGCCAC CAGCAGCGAC AACTTCAAGG CCCTCCTGCT TCGGAAGGGA 
AGCCGCTCTG AGAACAGTTT CCGCATGTCT GCTACTGAAA TGCTGCGCTC CACCGACCCG 
CGCTTCCAGC GAACTCGCTC CGAGTCTGCG CTGGACCCCC CCACTGCGTC TCCCACGTCA 
CCGATGGCGC CTCACAGCCC CTCTGCCTCT TTTGGGCGGA GTAAGAGGGT AACGGATGAG 
TGGAGCCGCT ACGATGCCCT GTCCTCGCCG ACATATTCGC CTGGATCAAA GTTCGGGCGC 
TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC AAATACAACA CTCGCAGCCG AATCCTCAGC 
AGCCCGATGA CTGTGATCTG TGAGCGTGAA GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG 
GTCGGCGAGA GCCGGGAAGG AGTCCAGACG CTTCCAGAAA CCAACGGCAC TTTATCCGAA 
GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714404.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570063.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OJa76381
Clone ID Related Accession (Same CDS sequence)	XM_020714404.1
Accession Version	XM_020714404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGGGGCAGT GTTTGACCGC 
CAGGCGAGCA TCCGGCGCTC CCTCATTAAC ACTGATACCG TGTCCCGTCG GCCCAAGAAG 
GTCAAACGCA GAAAGACTAT ATCAGGGCTG CCTGACAACT TCAACCAGGA GCTAGCTAAA 
GGACGAGGCG GGGAACTTCG GCCACATTCC ATGTTCATCC CAGGACAGTA CTCCACTTTG 
GGGAGAGTTG GGAGTGTCAA CTCAACACTC CGCTTCGAGA AAAGAGACTC CAGCTGCCAG 
ACAGAAGAAG TGAAGATTGT CCCTCCATCT GTGAGAAGAA TTCGAGCACA AAGAGGGCAG 
GGAATCGCCG CTCAAATGGC TGGGATATCT GCTTCCTCTT CTACTGGAAG CATCTCCATC 
TCGAGCACAG ATGGCTTGAT TCTGCCCAAT CAGTTTTTCA GAGACCCTTC CCGCTTTCAC 
AGTCTGCCGC GACAGGGTGC CAGAGTGTCC CTCAGTGCTG ATCCCATCTA TAGCAGCACG 
CCCATTAAGT CAGAGGATCA GTTGCAGAGG AATATTGGAA AATTCCAGGT TGACGAAACA 
GCGGTACACA TCAGGAATGC CCCACGAACG AGCACCTTGC CCAGACCCAC GTCTCAGGAG 
GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA GGTGGTCCAG CATGTGTGGT CTCCCCTCAG 
GCAGCTTATT CCACCTCCTT CATTCCCAAT GCCACCCTGT CCAACTCCAC GGATGTTATC 
ACCCTCAGCT CATCTAGTCA TTTATCCTTA TCAGCTGTCT CAGCTTTGGC CACTTCTACC 
CCCACGCGTG CACCACAAGA GAACGGTGTG ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC 
CACTTGGATG GAAGTGTTCA TAGTCACAGT ACTTTGGCTC CCACTTCACC ATCATGTCCT 
CCAGAAGAAC AGTGGATCTA TGACTCACCC GAAAATGTGG TTCCACACCG CACTCTGACC 
TCCAGCTGTT CCACTCCCAT CAACCAGCTT TATAGCAGCC TGGACCATTC CTCACGGACT 
GATTCCAGCT CCCTCTACTC CCAGGACAAC GATGGATACT TCACCTCTAT GCACATGGAC 
TCAGGGCTGC GCTCCCGAAG TCACGGAAGT GGGCACGGCG CAGGAGCTGG GCGGTCCCGA 
CACAGCATGT ATGAGTGTCG CGAGATGGCC AATCGAGAAG ACTCTGGAAG CGTGTACAGC 
GACCGCTCTC TGTCGCGCAG CATCTCGCTC CGCAAGTCAA AGAAGCCACC GCCACCTCCG 
GCACGCACAG ATTCTCTGAG GCGCAAGCCT GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT 
CCAACAACTG TTGCCAATGG GGACGGTGCT AACATGCTCA ATGAAACGTT CCTTGCTAGC 
TTGCAGCAGA GCCTGCAAAT GGGACTGAGG GGGTCAAAGG GAAAGGGTAC CTCTCTATCT 
TCACCCTCTC ACAGCCCCAG CAGTGACTAT GATGACCCTT GGTTGTTGCG ACCGCGCAGT 
CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC GCCTCATTAG CTAACACAAA TGGTGTGTCT 
AATGTCTACT CATTATGCCA TGTCACGCCC ACGCATAGTG ACACCAGCAG TTTGCGTTCT 
GACTATGCAG AGTCCTGGGG ATACTACATG GACTACCCTC GTAACCCTGG AGACCAGGGG 
GGGCAGACCC CACCAGCCCA TGCTACAGAT AACATGTCAG CTGGGGGTCA TCCAGGAGTC 
CTACAGAATG GAGGAGAGAT TCACAGCAAT ACTCAAACAG ATGGAGCTCC AGGCCAGGAG 
GGAGGGCAGT CAGGGAAGCC CAAACCATCC AGCTCCTCAC CGGACAGGGT GCACAGACTT 
ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG TCCAACACGC CTACAGCCGG AACCCCGGTG 
CCAGCCTTCG TCAGATCCAT GTCTCCCTCC TCAGGGAGTC GGCCCAAACC CAAAGTGCCG 
GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC 
TCCAACACTT CAGACTCACT AAAGAGTTCA GGTCCTCCTC TGCCACCCCC TAATCCCCTC 
CATCTCACCT CTTCTACCCC AAACACCCCT CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT 
CTTCCTCCAA ATTCTACATC AGCTCCCTCA TCTCCTCCTC CACCTCCTCC ATTACCCAAC 
TCTCCAAGAG AAACCTTCTT GGCCCCTCTA TCTGCGTCAC CAGAATTCCC TCCTCCTCCA 
TCTCCAGACC TGTTGATGCA CCACAGTTCG TCCTTAAATG GGAGCTTTAG TCCTCCACCT 
CCCCCTGAGA CCGGGATCCC TCCACCACCT CCACTGCCTG ACTTCACCCC AGCTTTTGGA 
TCCCCAAATA TTAGGAAATT GACAAAAAAT ACTCCTAAAC CACATCCTTC CAAACACTCC 
ACAGAGTCCC CCAAGCCCCT CATCACCCCC TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC 
GTCAAGCGAT CACAAGAGGA GATCACTGGG GAGCTGGACC AAATGAAACC TCAGGAAAAT 
GGACTGGACC TTCTCCAGGA GCTGAAACTC CATAGTCTAG ATCCATCCCC CATCCAGGAG 
CACACCACTG TCACGAGTAG CTCTCCAGAC GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT 
GTGACGAAAC TGCTGGAAGA GCTGAGTCTG GACTGCAGTT TTATAATCGA CACACAGGAT 
AGTGGTGTCA TCAATGGAAA AGCTGAGGAC CAGAGTTACT GTCTATCAAA TGGAAAAGAC 
AATGAGGAAG AGCTGCCACT GCCTAGCTGT CCAGAGAGAT GTGGAAGCTC CTCCATCAAA 
GCCAAGCCTC CGCCAGTCTC CAAGAAACCC AAACTTACCT TTGTTCCATC CCAACCAATC 
AGCGAGCCAG CTCAAACTCA AAATGACAAT GCAGCCAGTC TGTCGCCAGA GGATCAGGTC 
GATGCACAAA CGACAAATGA CGTAACAAAT AAGAGGAACA ACCGGGAGGA AGAGGCGTCA 
GAGAGCACAA AGGCTTCCAC TGAGAACCTG GACCAGAGCT GCCAAGATGC AGATCTTGAC 
CACGACATTT GCACTAATGG AGAGGTGTTT GATGAGGATG AAGAAGAGGA AGAAGGAGAA 
GGAGCAAGCA GCACCACGGG GTCCATCAGC TCAAAAGAGG AAGACACAAG TGANGTTTTC 
GAATCCAGAC CGGCTGAACC TCCTTCACCC CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA 
ATGGTGACCC CGACTCCCTC CAAACCCCGA ACCACTGATG ACCTTTTTGC TGTCATACAC 
AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA GAGTCGGATG AAGACAAGTC CCGCCCCGGG 
GTTCAGGCAC AGTCTCCACC CTCCACCCCG TCACCCGTCT CTCCTGGGCC GGTTTCTCCG 
TCGGCCCGTC AGATGGGTTC CATCCAGCGA AACCTACGCA AGTCCGCCAC CAGCAGCGAC 
AACTTCAAGG CCCTCCTGCT TCGGAAGGGA AGCCGCTCTG AGAACAGTTT CCGCATGTCT 
GCTACTGAAA TGCTGCGCTC CACCGACCCG CGCTTCCAGC GAACTCGCTC CGAGTCTGCG 
CTGGACCCCC CCACTGCGTC TCCCACGTCA CCGATGGCGC CTCACAGCCC CTCTGCCTCT 
TTTGGGCGGA GTAAGAGGGT AACGGATGAG TGGAGCCGCT ACGATGCCCT GTCCTCGCCG 
ACATATTCGC CTGGATCAAA GTTCGGGCGC TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC 
AAATACAACA CTCGCAGCCG AATCCTCAGC AGCCCGATGA CTGTGATCTG TGAGCGTGAA 
GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG GTCGGCGAGA GCCGGGAAGG AGTCCAGACG 
CTTCCAGAAA CCAACGGCAC TTTATCCGAA GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570063.1
CDS	1138..5862
Translation	MKGGRRSASFHRERPAGLSRAFSWLSLSSLSEHRRRIFRSQHELHVPSPSSAHLEGSDRDGDDDSWVYKPQHKMAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714404.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714404.1

ORF Insert Sequence:

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGGGGCAGT GTTTGACCGC 
CAGGCGAGCA TCCGGCGCTC CCTCATTAAC ACTGATACCG TGTCCCGTCG GCCCAAGAAG 
GTCAAACGCA GAAAGACTAT ATCAGGGCTG CCTGACAACT TCAACCAGGA GCTAGCTAAA 
GGACGAGGCG GGGAACTTCG GCCACATTCC ATGTTCATCC CAGGACAGTA CTCCACTTTG 
GGGAGAGTTG GGAGTGTCAA CTCAACACTC CGCTTCGAGA AAAGAGACTC CAGCTGCCAG 
ACAGAAGAAG TGAAGATTGT CCCTCCATCT GTGAGAAGAA TTCGAGCACA AAGAGGGCAG 
GGAATCGCCG CTCAAATGGC TGGGATATCT GCTTCCTCTT CTACTGGAAG CATCTCCATC 
TCGAGCACAG ATGGCTTGAT TCTGCCCAAT CAGTTTTTCA GAGACCCTTC CCGCTTTCAC 
AGTCTGCCGC GACAGGGTGC CAGAGTGTCC CTCAGTGCTG ATCCCATCTA TAGCAGCACG 
CCCATTAAGT CAGAGGATCA GTTGCAGAGG AATATTGGAA AATTCCAGGT TGACGAAACA 
GCGGTACACA TCAGGAATGC CCCACGAACG AGCACCTTGC CCAGACCCAC GTCTCAGGAG 
GTGAGGAGGA CGCAGTCCAG CGAGTGGGGA GGTGGTCCAG CATGTGTGGT CTCCCCTCAG 
GCAGCTTATT CCACCTCCTT CATTCCCAAT GCCACCCTGT CCAACTCCAC GGATGTTATC 
ACCCTCAGCT CATCTAGTCA TTTATCCTTA TCAGCTGTCT CAGCTTTGGC CACTTCTACC 
CCCACGCGTG CACCACAAGA GAACGGTGTG ACGCCTTCAG GCCCCCCCAG TGAGTCGGGC 
CACTTGGATG GAAGTGTTCA TAGTCACAGT ACTTTGGCTC CCACTTCACC ATCATGTCCT 
CCAGAAGAAC AGTGGATCTA TGACTCACCC GAAAATGTGG TTCCACACCG CACTCTGACC 
TCCAGCTGTT CCACTCCCAT CAACCAGCTT TATAGCAGCC TGGACCATTC CTCACGGACT 
GATTCCAGCT CCCTCTACTC CCAGGACAAC GATGGATACT TCACCTCTAT GCACATGGAC 
TCAGGGCTGC GCTCCCGAAG TCACGGAAGT GGGCACGGCG CAGGAGCTGG GCGGTCCCGA 
CACAGCATGT ATGAGTGTCG CGAGATGGCC AATCGAGAAG ACTCTGGAAG CGTGTACAGC 
GACCGCTCTC TGTCGCGCAG CATCTCGCTC CGCAAGTCAA AGAAGCCACC GCCACCTCCG 
GCACGCACAG ATTCTCTGAG GCGCAAGCCT GCCGCAAAAA AGCCCTTAGG TGGTGTTGGT 
CCAACAACTG TTGCCAATGG GGACGGTGCT AACATGCTCA ATGAAACGTT CCTTGCTAGC 
TTGCAGCAGA GCCTGCAAAT GGGACTGAGG GGGTCAAAGG GAAAGGGTAC CTCTCTATCT 
TCACCCTCTC ACAGCCCCAG CAGTGACTAT GATGACCCTT GGTTGTTGCG ACCGCGCAGT 
CAGAGTAGCA TCAGTGCAGG CAGCTCTGCC GCCTCATTAG CTAACACAAA TGGTGTGTCT 
AATGTCTACT CATTATGCCA TGTCACGCCC ACGCATAGTG ACACCAGCAG TTTGCGTTCT 
GACTATGCAG AGTCCTGGGG ATACTACATG GACTACCCTC GTAACCCTGG AGACCAGGGG 
GGGCAGACCC CACCAGCCCA TGCTACAGAT AACATGTCAG CTGGGGGTCA TCCAGGAGTC 
CTACAGAATG GAGGAGAGAT TCACAGCAAT ACTCAAACAG ATGGAGCTCC AGGCCAGGAG 
GGAGGGCAGT CAGGGAAGCC CAAACCATCC AGCTCCTCAC CGGACAGGGT GCACAGACTT 
ACCTCCCCGT CAAGCGGTTA CTCCAGCCAG TCCAACACGC CTACAGCCGG AACCCCGGTG 
CCAGCCTTCG TCAGATCCAT GTCTCCCTCC TCAGGGAGTC GGCCCAAACC CAAAGTGCCG 
GAGAGGAAGT CCTCGCTCCT CTCTTCGGGT TCTGTCTCTT CGTCCTCTAC TTCTTTGTCC 
TCCAACACTT CAGACTCACT AAAGAGTTCA GGTCCTCCTC TGCCACCCCC TAATCCCCTC 
CATCTCACCT CTTCTACCCC AAACACCCCT CTTGGTCCTC CTCCGCCCTT TCCTCCTCCT 
CTTCCTCCAA ATTCTACATC AGCTCCCTCA TCTCCTCCTC CACCTCCTCC ATTACCCAAC 
TCTCCAAGAG AAACCTTCTT GGCCCCTCTA TCTGCGTCAC CAGAATTCCC TCCTCCTCCA 
TCTCCAGACC TGTTGATGCA CCACAGTTCG TCCTTAAATG GGAGCTTTAG TCCTCCACCT 
CCCCCTGAGA CCGGGATCCC TCCACCACCT CCACTGCCTG ACTTCACCCC AGCTTTTGGA 
TCCCCAAATA TTAGGAAATT GACAAAAAAT ACTCCTAAAC CACATCCTTC CAAACACTCC 
ACAGAGTCCC CCAAGCCCCT CATCACCCCC TTCGCCCTGC AGAGTGTCCA GCTGCGCTCC 
GTCAAGCGAT CACAAGAGGA GATCACTGGG GAGCTGGACC AAATGAAACC TCAGGAAAAT 
GGACTGGACC TTCTCCAGGA GCTGAAACTC CATAGTCTAG ATCCATCCCC CATCCAGGAG 
CACACCACTG TCACGAGTAG CTCTCCAGAC GAAGAGCTGC GGGAGTCCAC ACCTTCGCCT 
GTGACGAAAC TGCTGGAAGA GCTGAGTCTG GACTGCAGTT TTATAATCGA CACACAGGAT 
AGTGGTGTCA TCAATGGAAA AGCTGAGGAC CAGAGTTACT GTCTATCAAA TGGAAAAGAC 
AATGAGGAAG AGCTGCCACT GCCTAGCTGT CCAGAGAGAT GTGGAAGCTC CTCCATCAAA 
GCCAAGCCTC CGCCAGTCTC CAAGAAACCC AAACTTACCT TTGTTCCATC CCAACCAATC 
AGCGAGCCAG CTCAAACTCA AAATGACAAT GCAGCCAGTC TGTCGCCAGA GGATCAGGTC 
GATGCACAAA CGACAAATGA CGTAACAAAT AAGAGGAACA ACCGGGAGGA AGAGGCGTCA 
GAGAGCACAA AGGCTTCCAC TGAGAACCTG GACCAGAGCT GCCAAGATGC AGATCTTGAC 
CACGACATTT GCACTAATGG AGAGGTGTTT GATGAGGATG AAGAAGAGGA AGAAGGAGAA 
GGAGCAAGCA GCACCACGGG GTCCATCAGC TCAAAAGAGG AAGACACAAG TGANGTTTTC 
GAATCCAGAC CGGCTGAACC TCCTTCACCC CAGTCGGCCG TAGGGGCAAG CGAGCAAAAA 
ATGGTGACCC CGACTCCCTC CAAACCCCGA ACCACTGATG ACCTTTTTGC TGTCATACAC 
AGGTCAAAGC GAAAGGTTCT CGGTCGCAGA GAGTCGGATG AAGACAAGTC CCGCCCCGGG 
GTTCAGGCAC AGTCTCCACC CTCCACCCCG TCACCCGTCT CTCCTGGGCC GGTTTCTCCG 
TCGGCCCGTC AGATGGGTTC CATCCAGCGA AACCTACGCA AGTCCGCCAC CAGCAGCGAC 
AACTTCAAGG CCCTCCTGCT TCGGAAGGGA AGCCGCTCTG AGAACAGTTT CCGCATGTCT 
GCTACTGAAA TGCTGCGCTC CACCGACCCG CGCTTCCAGC GAACTCGCTC CGAGTCTGCG 
CTGGACCCCC CCACTGCGTC TCCCACGTCA CCGATGGCGC CTCACAGCCC CTCTGCCTCT 
TTTGGGCGGA GTAAGAGGGT AACGGATGAG TGGAGCCGCT ACGATGCCCT GTCCTCGCCG 
ACATATTCGC CTGGATCAAA GTTCGGGCGC TCTCGCGCGC CGCCTTCCGC CGCCAGCAGC 
AAATACAACA CTCGCAGCCG AATCCTCAGC AGCCCGATGA CTGTGATCTG TGAGCGTGAA 
GGGGAGCTGA CTGAGAGCGA ATCTGGAGAG GTCGGCGAGA GCCGGGAAGG AGTCCAGACG 
CTTCCAGAAA CCAACGGCAC TTTATCCGAA GAGAGCAGAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714405.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570064.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.

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CloneID	OJa76382
Clone ID Related Accession (Same CDS sequence)	XM_020714405.1
Accession Version	XM_020714405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGCTAAAGGA CGAGGCGGGG AACTTCGGCC ACATTCCATG 
TTCATCCCAG GACAGTACTC CACTTTGGGG AGAGTTGGGA GTGTCAACTC AACACTCCGC 
TTCGAGAAAA GAGACTCCAG CTGCCAGACA GAAGAAGTGA AGATTGTCCC TCCATCTGTG 
AGAAGAATTC GAGCACAAAG AGGGCAGGGA ATCGCCGCTC AAATGGCTGG GATATCTGCT 
TCCTCTTCTA CTGGAAGCAT CTCCATCTCG AGCACAGATG GCTTGATTCT GCCCAATCAG 
TTTTTCAGAG ACCCTTCCCG CTTTCACAGT CTGCCGCGAC AGGGTGCCAG AGTGTCCCTC 
AGTGCTGATC CCATCTATAG CAGCACGCCC ATTAAGTCAG AGGATCAGTT GCAGAGGAAT 
ATTGGAAAAT TCCAGGTTGA CGAAACAGCG GTACACATCA GGAATGCCCC ACGAACGAGC 
ACCTTGCCCA GACCCACGTC TCAGGAGGTG AGGAGGACGC AGTCCAGCGA GTGGGGAGGT 
GGTCCAGCAT GTGTGGTCTC CCCTCAGGCA GCTTATTCCA CCTCCTTCAT TCCCAATGCC 
ACCCTGTCCA ACTCCACGGA TGTTATCACC CTCAGCTCAT CTAGTCATTT ATCCTTATCA 
GCTGTCTCAG CTTTGGCCAC TTCTACCCCC ACGCGTGCAC CACAAGAGAA CGGTGTGACG 
CCTTCAGGCC CCCCCAGTGA GTCGGGCCAC TTGGATGGAA GTGTTCATAG TCACAGTACT 
TTGGCTCCCA CTTCACCATC ATGTCCTCCA GAAGAACAGT GGATCTATGA CTCACCCGAA 
AATGTGGTTC CACACCGCAC TCTGACCTCC AGCTGTTCCA CTCCCATCAA CCAGCTTTAT 
AGCAGCCTGG ACCATTCCTC ACGGACTGAT TCCAGCTCCC TCTACTCCCA GGACAACGAT 
GGATACTTCA CCTCTATGCA CATGGACTCA GGGCTGCGCT CCCGAAGTCA CGGAAGTGGG 
CACGGCGCAG GAGCTGGGCG GTCCCGACAC AGCATGTATG AGTGTCGCGA GATGGCCAAT 
CGAGAAGACT CTGGAAGCGT GTACAGCGAC CGCTCTCTGT CGCGCAGCAT CTCGCTCCGC 
AAGTCAAAGA AGCCACCGCC ACCTCCGGCA CGCACAGATT CTCTGAGGCG CAAGCCTGCC 
GCAAAAAAGC CCTTAGGTGG TGTTGGTCCA ACAACTGTTG CCAATGGGGA CGGTGCTAAC 
ATGCTCAATG AAACGTTCCT TGCTAGCTTG CAGCAGAGCC TGCAAATGGG ACTGAGGGGG 
TCAAAGGGAA AGGGTACCTC TCTATCTTCA CCCTCTCACA GCCCCAGCAG TGACTATGAT 
GACCCTTGGT TGTTGCGACC GCGCAGTCAG AGTAGCATCA GTGCAGGCAG CTCTGCCGCC 
TCATTAGCTA ACACAAATGG TGTGTCTAAT GTCTACTCAT TATGCCATGT CACGCCCACG 
CATAGTGACA CCAGCAGTTT GCGTTCTGAC TATGCAGAGT CCTGGGGATA CTACATGGAC 
TACCCTCGTA ACCCTGGAGA CCAGGGGGGG CAGACCCCAC CAGCCCATGC TACAGATAAC 
ATGTCAGCTG GGGGTCATCC AGGAGTCCTA CAGAATGGAG GAGAGATTCA CAGCAATACT 
CAAACAGATG GAGCTCCAGG CCAGGAGGGA GGGCAGTCAG GGAAGCCCAA ACCATCCAGC 
TCCTCACCGG ACAGGGTGCA CAGACTTACC TCCCCGTCAA GCGGTTACTC CAGCCAGTCC 
AACACGCCTA CAGCCGGAAC CCCGGTGCCA GCCTTCGTCA GATCCATGTC TCCCTCCTCA 
GGGAGTCGGC CCAAACCCAA AGTGCCGGAG AGGAAGTCCT CGCTCCTCTC TTCGGGTTCT 
GTCTCTTCGT CCTCTACTTC TTTGTCCTCC AACACTTCAG ACTCACTAAA GAGTTCAGGT 
CCTCCTCTGC CACCCCCTAA TCCCCTCCAT CTCACCTCTT CTACCCCAAA CACCCCTCTT 
GGTCCTCCTC CGCCCTTTCC TCCTCCTCTT CCTCCAAATT CTACATCAGC TCCCTCATCT 
CCTCCTCCAC CTCCTCCATT ACCCAACTCT CCAAGAGAAA CCTTCTTGGC CCCTCTATCT 
GCGTCACCAG AATTCCCTCC TCCTCCATCT CCAGACCTGT TGATGCACCA CAGTTCGTCC 
TTAAATGGGA GCTTTAGTCC TCCACCTCCC CCTGAGACCG GGATCCCTCC ACCACCTCCA 
CTGCCTGACT TCACCCCAGC TTTTGGATCC CCAAATATTA GGAAATTGAC AAAAAATACT 
CCTAAACCAC ATCCTTCCAA ACACTCCACA GAGTCCCCCA AGCCCCTCAT CACCCCCTTC 
GCCCTGCAGA GTGTCCAGCT GCGCTCCGTC AAGCGATCAC AAGAGGAGAT CACTGGGGAG 
CTGGACCAAA TGAAACCTCA GGAAAATGGA CTGGACCTTC TCCAGGAGCT GAAACTCCAT 
AGTCTAGATC CATCCCCCAT CCAGGAGCAC ACCACTGTCA CGAGTAGCTC TCCAGACGAA 
GAGCTGCGGG AGTCCACACC TTCGCCTGTG ACGAAACTGC TGGAAGAGCT GAGTCTGGAC 
TGCAGTTTTA TAATCGACAC ACAGGATAGT GGTGTCATCA ATGGAAAAGC TGAGGACCAG 
AGTTACTGTC TATCAAATGG AAAAGACAAT GAGGAAGAGC TGCCACTGCC TAGCTGTCCA 
GAGAGATGTG GAAGCTCCTC CATCAAAGCC AAGCCTCCGC CAGTCTCCAA GAAACCCAAA 
CTTACCTTTG TTCCATCCCA ACCAATCAGC GAGCCAGCTC AAACTCAAAA TGACAATGCA 
GCCAGTCTGT CGCCAGAGGA TCAGGTCGAT GCACAAACGA CAAATGACGT AACAAATAAG 
AGGAACAACC GGGAGGAAGA GGCGTCAGAG AGCACAAAGG CTTCCACTGA GAACCTGGAC 
CAGAGCTGCC AAGATGCAGA TCTTGACCAC GACATTTGCA CTAATGGAGA GGTGTTTGAT 
GAGGATGAAG AAGAGGAAGA AGGAGAAGGA GCAAGCAGCA CCACGGGGTC CATCAGCTCA 
AAAGAGGAAG ACACAAGTGA NGTTTTCGAA TCCAGACCGG CTGAACCTCC TTCACCCCAG 
TCGGCCGTAG GGGCAAGCGA GCAAAAAATG GTGACCCCGA CTCCCTCCAA ACCCCGAACC 
ACTGATGACC TTTTTGCTGT CATACACAGG TCAAAGCGAA AGGTTCTCGG TCGCAGAGAG 
TCGGATGAAG ACAAGTCCCG CCCCGGGGTT CAGGCACAGT CTCCACCCTC CACCCCGTCA 
CCCGTCTCTC CTGGGCCGGT TTCTCCGTCG GCCCGTCAGA TGGGTTCCAT CCAGCGAAAC 
CTACGCAAGT CCGCCACCAG CAGCGACAAC TTCAAGGCCC TCCTGCTTCG GAAGGGAAGC 
CGCTCTGAGA ACAGTTTCCG CATGTCTGCT ACTGAAATGC TGCGCTCCAC CGACCCGCGC 
TTCCAGCGAA CTCGCTCCGA GTCTGCGCTG GACCCCCCCA CTGCGTCTCC CACGTCACCG 
ATGGCGCCTC ACAGCCCCTC TGCCTCTTTT GGGCGGAGTA AGAGGGTAAC GGATGAGTGG 
AGCCGCTACG ATGCCCTGTC CTCGCCGACA TATTCGCCTG GATCAAAGTT CGGGCGCTCT 
CGCGCGCCGC CTTCCGCCGC CAGCAGCAAA TACAACACTC GCAGCCGAAT CCTCAGCAGC 
CCGATGACTG TGATCTGTGA GCGTGAAGGG GAGCTGACTG AGAGCGAATC TGGAGAGGTC 
GGCGAGAGCC GGGAAGGAGT CCAGACGCTT CCAGAAACCA ACGGCACTTT ATCCGAAGAG 
AGCAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570064.1
CDS	344..5035
Translation	MPFHQRTVEPRRVSRLSARYGWKAREEEPGKRKTRRLVLFSSLDEVSCHTLTGLIHQLSDLSRHASDIFLGIEVEAGAVFRRSCRIQGRLQFLQDEVCRLDPKKVKIPVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714405.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714405.1

ORF Insert Sequence:

ATGCCCTTCC ATCAGAGGAC TGTTGAACCC CGCCGAGTGA GTCGGTTATC GGCGAGGTAC 
GGCTGGAAAG CGAGGGAAGA AGAACCGGGC AAACGCAAAA CCCGACGGCT GGTTCTCTTC 
TCCTCCTTGG ACGAAGTTAG CTGTCACACT TTGACCGGCT TGATCCACCA ACTTTCAGAC 
CTTTCCCGGC ATGCCAGCGA CATCTTCCTG GGCATCGAGG TGGAGGCAGG AGCGGTCTTC 
AGGAGATCCT GCAGGATCCA GGGGCGCCTG CAATTTCTGC AGGATGAAGT CTGCAGACTG 
GACCCCAAGA AAGTCAAAAT CCCTGTGTCA AACCTGGATG AGGAGAGCAA ATGGACGGTA 
CACTACACGG CTCCTTGGCA CCAGCAGGAA AACGTCTTCC TGCCGGGCAG CAGGCCGTCC 
TGCGTGGAGG ACCTCCACCG CCAAGCCAAA GTCAACCTCA AGACCACCCT GCGAGAATGT 
GATAAGTTGA GGAAAGATGG TTTCCGGAGC TCCCAGTACT ACTCTCAGGG CCCGACCTTC 
TCCGAGCCCT TACAGTCCAG CAGCAGCCTG CAAGACGAGG AAGATGAGGA AAATGACAAG 
AAGTCTTTAG CATCCTCGAT GGAGGATGAT AAATCCCAGC TCTCGCTGAG GTTGCAGGAG 
CGCTGCGGTG AAAGGGGCGA GATGTTGGAA GTTGACGGGC AGATGGTGTG GAATAAAGCG 
GTTCCCCTCC CGACGCCGGA GGAGAGGATG AGGCAAGCAG CTCAGGCCGT GCCCACGGAC 
ATAGTCCCCA TCAATGTCAC AGCTAAAGGA CGAGGCGGGG AACTTCGGCC ACATTCCATG 
TTCATCCCAG GACAGTACTC CACTTTGGGG AGAGTTGGGA GTGTCAACTC AACACTCCGC 
TTCGAGAAAA GAGACTCCAG CTGCCAGACA GAAGAAGTGA AGATTGTCCC TCCATCTGTG 
AGAAGAATTC GAGCACAAAG AGGGCAGGGA ATCGCCGCTC AAATGGCTGG GATATCTGCT 
TCCTCTTCTA CTGGAAGCAT CTCCATCTCG AGCACAGATG GCTTGATTCT GCCCAATCAG 
TTTTTCAGAG ACCCTTCCCG CTTTCACAGT CTGCCGCGAC AGGGTGCCAG AGTGTCCCTC 
AGTGCTGATC CCATCTATAG CAGCACGCCC ATTAAGTCAG AGGATCAGTT GCAGAGGAAT 
ATTGGAAAAT TCCAGGTTGA CGAAACAGCG GTACACATCA GGAATGCCCC ACGAACGAGC 
ACCTTGCCCA GACCCACGTC TCAGGAGGTG AGGAGGACGC AGTCCAGCGA GTGGGGAGGT 
GGTCCAGCAT GTGTGGTCTC CCCTCAGGCA GCTTATTCCA CCTCCTTCAT TCCCAATGCC 
ACCCTGTCCA ACTCCACGGA TGTTATCACC CTCAGCTCAT CTAGTCATTT ATCCTTATCA 
GCTGTCTCAG CTTTGGCCAC TTCTACCCCC ACGCGTGCAC CACAAGAGAA CGGTGTGACG 
CCTTCAGGCC CCCCCAGTGA GTCGGGCCAC TTGGATGGAA GTGTTCATAG TCACAGTACT 
TTGGCTCCCA CTTCACCATC ATGTCCTCCA GAAGAACAGT GGATCTATGA CTCACCCGAA 
AATGTGGTTC CACACCGCAC TCTGACCTCC AGCTGTTCCA CTCCCATCAA CCAGCTTTAT 
AGCAGCCTGG ACCATTCCTC ACGGACTGAT TCCAGCTCCC TCTACTCCCA GGACAACGAT 
GGATACTTCA CCTCTATGCA CATGGACTCA GGGCTGCGCT CCCGAAGTCA CGGAAGTGGG 
CACGGCGCAG GAGCTGGGCG GTCCCGACAC AGCATGTATG AGTGTCGCGA GATGGCCAAT 
CGAGAAGACT CTGGAAGCGT GTACAGCGAC CGCTCTCTGT CGCGCAGCAT CTCGCTCCGC 
AAGTCAAAGA AGCCACCGCC ACCTCCGGCA CGCACAGATT CTCTGAGGCG CAAGCCTGCC 
GCAAAAAAGC CCTTAGGTGG TGTTGGTCCA ACAACTGTTG CCAATGGGGA CGGTGCTAAC 
ATGCTCAATG AAACGTTCCT TGCTAGCTTG CAGCAGAGCC TGCAAATGGG ACTGAGGGGG 
TCAAAGGGAA AGGGTACCTC TCTATCTTCA CCCTCTCACA GCCCCAGCAG TGACTATGAT 
GACCCTTGGT TGTTGCGACC GCGCAGTCAG AGTAGCATCA GTGCAGGCAG CTCTGCCGCC 
TCATTAGCTA ACACAAATGG TGTGTCTAAT GTCTACTCAT TATGCCATGT CACGCCCACG 
CATAGTGACA CCAGCAGTTT GCGTTCTGAC TATGCAGAGT CCTGGGGATA CTACATGGAC 
TACCCTCGTA ACCCTGGAGA CCAGGGGGGG CAGACCCCAC CAGCCCATGC TACAGATAAC 
ATGTCAGCTG GGGGTCATCC AGGAGTCCTA CAGAATGGAG GAGAGATTCA CAGCAATACT 
CAAACAGATG GAGCTCCAGG CCAGGAGGGA GGGCAGTCAG GGAAGCCCAA ACCATCCAGC 
TCCTCACCGG ACAGGGTGCA CAGACTTACC TCCCCGTCAA GCGGTTACTC CAGCCAGTCC 
AACACGCCTA CAGCCGGAAC CCCGGTGCCA GCCTTCGTCA GATCCATGTC TCCCTCCTCA 
GGGAGTCGGC CCAAACCCAA AGTGCCGGAG AGGAAGTCCT CGCTCCTCTC TTCGGGTTCT 
GTCTCTTCGT CCTCTACTTC TTTGTCCTCC AACACTTCAG ACTCACTAAA GAGTTCAGGT 
CCTCCTCTGC CACCCCCTAA TCCCCTCCAT CTCACCTCTT CTACCCCAAA CACCCCTCTT 
GGTCCTCCTC CGCCCTTTCC TCCTCCTCTT CCTCCAAATT CTACATCAGC TCCCTCATCT 
CCTCCTCCAC CTCCTCCATT ACCCAACTCT CCAAGAGAAA CCTTCTTGGC CCCTCTATCT 
GCGTCACCAG AATTCCCTCC TCCTCCATCT CCAGACCTGT TGATGCACCA CAGTTCGTCC 
TTAAATGGGA GCTTTAGTCC TCCACCTCCC CCTGAGACCG GGATCCCTCC ACCACCTCCA 
CTGCCTGACT TCACCCCAGC TTTTGGATCC CCAAATATTA GGAAATTGAC AAAAAATACT 
CCTAAACCAC ATCCTTCCAA ACACTCCACA GAGTCCCCCA AGCCCCTCAT CACCCCCTTC 
GCCCTGCAGA GTGTCCAGCT GCGCTCCGTC AAGCGATCAC AAGAGGAGAT CACTGGGGAG 
CTGGACCAAA TGAAACCTCA GGAAAATGGA CTGGACCTTC TCCAGGAGCT GAAACTCCAT 
AGTCTAGATC CATCCCCCAT CCAGGAGCAC ACCACTGTCA CGAGTAGCTC TCCAGACGAA 
GAGCTGCGGG AGTCCACACC TTCGCCTGTG ACGAAACTGC TGGAAGAGCT GAGTCTGGAC 
TGCAGTTTTA TAATCGACAC ACAGGATAGT GGTGTCATCA ATGGAAAAGC TGAGGACCAG 
AGTTACTGTC TATCAAATGG AAAAGACAAT GAGGAAGAGC TGCCACTGCC TAGCTGTCCA 
GAGAGATGTG GAAGCTCCTC CATCAAAGCC AAGCCTCCGC CAGTCTCCAA GAAACCCAAA 
CTTACCTTTG TTCCATCCCA ACCAATCAGC GAGCCAGCTC AAACTCAAAA TGACAATGCA 
GCCAGTCTGT CGCCAGAGGA TCAGGTCGAT GCACAAACGA CAAATGACGT AACAAATAAG 
AGGAACAACC GGGAGGAAGA GGCGTCAGAG AGCACAAAGG CTTCCACTGA GAACCTGGAC 
CAGAGCTGCC AAGATGCAGA TCTTGACCAC GACATTTGCA CTAATGGAGA GGTGTTTGAT 
GAGGATGAAG AAGAGGAAGA AGGAGAAGGA GCAAGCAGCA CCACGGGGTC CATCAGCTCA 
AAAGAGGAAG ACACAAGTGA NGTTTTCGAA TCCAGACCGG CTGAACCTCC TTCACCCCAG 
TCGGCCGTAG GGGCAAGCGA GCAAAAAATG GTGACCCCGA CTCCCTCCAA ACCCCGAACC 
ACTGATGACC TTTTTGCTGT CATACACAGG TCAAAGCGAA AGGTTCTCGG TCGCAGAGAG 
TCGGATGAAG ACAAGTCCCG CCCCGGGGTT CAGGCACAGT CTCCACCCTC CACCCCGTCA 
CCCGTCTCTC CTGGGCCGGT TTCTCCGTCG GCCCGTCAGA TGGGTTCCAT CCAGCGAAAC 
CTACGCAAGT CCGCCACCAG CAGCGACAAC TTCAAGGCCC TCCTGCTTCG GAAGGGAAGC 
CGCTCTGAGA ACAGTTTCCG CATGTCTGCT ACTGAAATGC TGCGCTCCAC CGACCCGCGC 
TTCCAGCGAA CTCGCTCCGA GTCTGCGCTG GACCCCCCCA CTGCGTCTCC CACGTCACCG 
ATGGCGCCTC ACAGCCCCTC TGCCTCTTTT GGGCGGAGTA AGAGGGTAAC GGATGAGTGG 
AGCCGCTACG ATGCCCTGTC CTCGCCGACA TATTCGCCTG GATCAAAGTT CGGGCGCTCT 
CGCGCGCCGC CTTCCGCCGC CAGCAGCAAA TACAACACTC GCAGCCGAAT CCTCAGCAGC 
CCGATGACTG TGATCTGTGA GCGTGAAGGG GAGCTGACTG AGAGCGAATC TGGAGAGGTC 
GGCGAGAGCC GGGAAGGAGT CCAGACGCTT CCAGAAACCA ACGGCACTTT ATCCGAAGAG 
AGCAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714406.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570065.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.

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CloneID	OJa76383
Clone ID Related Accession (Same CDS sequence)	XM_020714406.1
Accession Version	XM_020714406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGCTAAAGG ACGAGGCGGG 
GAACTTCGGC CACATTCCAT GTTCATCCCA GGACAGTACT CCACTTTGGG GAGAGTTGGG 
AGTGTCAACT CAACACTCCG CTTCGAGAAA AGAGACTCCA GCTGCCAGAC AGAAGAAGTG 
AAGATTGTCC CTCCATCTGT GAGAAGAATT CGAGCACAAA GAGGGCAGGG AATCGCCGCT 
CAAATGGCTG GGATATCTGC TTCCTCTTCT ACTGGAAGCA TCTCCATCTC GAGCACAGAT 
GGCTTGATTC TGCCCAATCA GTTTTTCAGA GACCCTTCCC GCTTTCACAG TCTGCCGCGA 
CAGGGTGCCA GAGTGTCCCT CAGTGCTGAT CCCATCTATA GCAGCACGCC CATTAAGTCA 
GAGGATCAGT TGCAGAGGAA TATTGGAAAA TTCCAGGTTG ACGAAACAGC GGTACACATC 
AGGAATGCCC CACGAACGAG CACCTTGCCC AGACCCACGT CTCAGGAGGT GAGGAGGACG 
CAGTCCAGCG AGTGGGGAGG TGGTCCAGCA TGTGTGGTCT CCCCTCAGGC AGCTTATTCC 
ACCTCCTTCA TTCCCAATGC CACCCTGTCC AACTCCACGG ATGTTATCAC CCTCAGCTCA 
TCTAGTCATT TATCCTTATC AGCTGTCTCA GCTTTGGCCA CTTCTACCCC CACGCGTGCA 
CCACAAGAGA ACGGTGTGAC GCCTTCAGGC CCCCCCAGTG AGTCGGGCCA CTTGGATGGA 
AGTGTTCATA GTCACAGTAC TTTGGCTCCC ACTTCACCAT CATGTCCTCC AGAAGAACAG 
TGGATCTATG ACTCACCCGA AAATGTGGTT CCACACCGCA CTCTGACCTC CAGCTGTTCC 
ACTCCCATCA ACCAGCTTTA TAGCAGCCTG GACCATTCCT CACGGACTGA TTCCAGCTCC 
CTCTACTCCC AGGACAACGA TGGATACTTC ACCTCTATGC ACATGGACTC AGGGCTGCGC 
TCCCGAAGTC ACGGAAGTGG GCACGGCGCA GGAGCTGGGC GGTCCCGACA CAGCATGTAT 
GAGTGTCGCG AGATGGCCAA TCGAGAAGAC TCTGGAAGCG TGTACAGCGA CCGCTCTCTG 
TCGCGCAGCA TCTCGCTCCG CAAGTCAAAG AAGCCACCGC CACCTCCGGC ACGCACAGAT 
TCTCTGAGGC GCAAGCCTGC CGCAAAAAAG CCCTTAGGTG GTGTTGGTCC AACAACTGTT 
GCCAATGGGG ACGGTGCTAA CATGCTCAAT GAAACGTTCC TTGCTAGCTT GCAGCAGAGC 
CTGCAAATGG GACTGAGGGG GTCAAAGGGA AAGGGTACCT CTCTATCTTC ACCCTCTCAC 
AGCCCCAGCA GTGACTATGA TGACCCTTGG TTGTTGCGAC CGCGCAGTCA GAGTAGCATC 
AGTGCAGGCA GCTCTGCCGC CTCATTAGCT AACACAAATG GTGTGTCTAA TGTCTACTCA 
TTATGCCATG TCACGCCCAC GCATAGTGAC ACCAGCAGTT TGCGTTCTGA CTATGCAGAG 
TCCTGGGGAT ACTACATGGA CTACCCTCGT AACCCTGGAG ACCAGGGGGG GCAGACCCCA 
CCAGCCCATG CTACAGATAA CATGTCAGCT GGGGGTCATC CAGGAGTCCT ACAGAATGGA 
GGAGAGATTC ACAGCAATAC TCAAACAGAT GGAGCTCCAG GCCAGGAGGG AGGGCAGTCA 
GGGAAGCCCA AACCATCCAG CTCCTCACCG GACAGGGTGC ACAGACTTAC CTCCCCGTCA 
AGCGGTTACT CCAGCCAGTC CAACACGCCT ACAGCCGGAA CCCCGGTGCC AGCCTTCGTC 
AGATCCATGT CTCCCTCCTC AGGGAGTCGG CCCAAACCCA AAGTGCCGGA GAGGAAGTCC 
TCGCTCCTCT CTTCGGGTTC TGTCTCTTCG TCCTCTACTT CTTTGTCCTC CAACACTTCA 
GACTCACTAA AGAGTTCAGG TCCTCCTCTG CCACCCCCTA ATCCCCTCCA TCTCACCTCT 
TCTACCCCAA ACACCCCTCT TGGTCCTCCT CCGCCCTTTC CTCCTCCTCT TCCTCCAAAT 
TCTACATCAG CTCCCTCATC TCCTCCTCCA CCTCCTCCAT TACCCAACTC TCCAAGAGAA 
ACCTTCTTGG CCCCTCTATC TGCGTCACCA GAATTCCCTC CTCCTCCATC TCCAGACCTG 
TTGATGCACC ACAGTTCGTC CTTAAATGGG AGCTTTAGTC CTCCACCTCC CCCTGAGACC 
GGGATCCCTC CACCACCTCC ACTGCCTGAC TTCACCCCAG CTTTTGGATC CCCAAATATT 
AGGAAATTGA CAAAAAATAC TCCTAAACCA CATCCTTCCA AACACTCCAC AGAGTCCCCC 
AAGCCCCTCA TCACCCCCTT CGCCCTGCAG AGTGTCCAGC TGCGCTCCGT CAAGCGATCA 
CAAGAGGAGA TCACTGGGGA GCTGGACCAA ATGAAACCTC AGGAAAATGG ACTGGACCTT 
CTCCAGGAGC TGAAACTCCA TAGTCTAGAT CCATCCCCCA TCCAGGAGCA CACCACTGTC 
ACGAGTAGCT CTCCAGACGA AGAGCTGCGG GAGTCCACAC CTTCGCCTGT GACGAAACTG 
CTGGAAGAGC TGAGTCTGGA CTGCAGTTTT ATAATCGACA CACAGGATAG TGGTGTCATC 
AATGGAAAAG CTGAGGACCA GAGTTACTGT CTATCAAATG GAAAAGACAA TGAGGAAGAG 
CTGCCACTGC CTAGCTGTCC AGAGAGATGT GGAAGCTCCT CCATCAAAGC CAAGCCTCCG 
CCAGTCTCCA AGAAACCCAA ACTTACCTTT GTTCCATCCC AACCAATCAG CGAGCCAGCT 
CAAACTCAAA ATGACAATGC AGCCAGTCTG TCGCCAGAGG ATCAGGTCGA TGCACAAACG 
ACAAATGACG TAACAAATAA GAGGAACAAC CGGGAGGAAG AGGCGTCAGA GAGCACAAAG 
GCTTCCACTG AGAACCTGGA CCAGAGCTGC CAAGATGCAG ATCTTGACCA CGACATTTGC 
ACTAATGGAG AGGTGTTTGA TGAGGATGAA GAAGAGGAAG AAGGAGAAGG AGCAAGCAGC 
ACCACGGGGT CCATCAGCTC AAAAGAGGAA GACACAAGTG ANGTTTTCGA ATCCAGACCG 
GCTGAACCTC CTTCACCCCA GTCGGCCGTA GGGGCAAGCG AGCAAAAAAT GGTGACCCCG 
ACTCCCTCCA AACCCCGAAC CACTGATGAC CTTTTTGCTG TCATACACAG GTCAAAGCGA 
AAGGTTCTCG GTCGCAGAGA GTCGGATGAA GACAAGTCCC GCCCCGGGGT TCAGGCACAG 
TCTCCACCCT CCACCCCGTC ACCCGTCTCT CCTGGGCCGG TTTCTCCGTC GGCCCGTCAG 
ATGGGTTCCA TCCAGCGAAA CCTACGCAAG TCCGCCACCA GCAGCGACAA CTTCAAGGCC 
CTCCTGCTTC GGAAGGGAAG CCGCTCTGAG AACAGTTTCC GCATGTCTGC TACTGAAATG 
CTGCGCTCCA CCGACCCGCG CTTCCAGCGA ACTCGCTCCG AGTCTGCGCT GGACCCCCCC 
ACTGCGTCTC CCACGTCACC GATGGCGCCT CACAGCCCCT CTGCCTCTTT TGGGCGGAGT 
AAGAGGGTAA CGGATGAGTG GAGCCGCTAC GATGCCCTGT CCTCGCCGAC ATATTCGCCT 
GGATCAAAGT TCGGGCGCTC TCGCGCGCCG CCTTCCGCCG CCAGCAGCAA ATACAACACT 
CGCAGCCGAA TCCTCAGCAG CCCGATGACT GTGATCTGTG AGCGTGAAGG GGAGCTGACT 
GAGAGCGAAT CTGGAGAGGT CGGCGAGAGC CGGGAAGGAG TCCAGACGCT TCCAGAAACC 
AACGGCACTT TATCCGAAGA GAGCAGAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570065.1
CDS	1138..5730
Translation	MKGGRRSASFHRERPAGLSRAFSWLSLSSLSEHRRRIFRSQHELHVPSPSSAHLEGSDRDGDDDSWVYKPQHKMAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714406.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714406.1

ORF Insert Sequence:

ATGAAAGGAG GCAGGAGATC TGCATCCTTC CACAGGGAGA GACCAGCAGG CCTGTCGCGG 
GCTTTCAGCT GGCTCAGCTT GTCCTCGCTG TCCGAGCACA GGAGACGGAT CTTCCGGAGC 
CAACACGAGC TTCACGTTCC TAGCCCCTCG AGCGCTCACC TCGAAGGCTC AGACAGAGAT 
GGGGACGACG ATAGCTGGGT GTACAAGCCT CAACACAAAA TGGCTGTGTC AAACCTGGAT 
GAGGAGAGCA AATGGACGGT ACACTACACG GCTCCTTGGC ACCAGCAGGA AAACGTCTTC 
CTGCCGGGCA GCAGGCCGTC CTGCGTGGAG GACCTCCACC GCCAAGCCAA AGTCAACCTC 
AAGACCACCC TGCGAGAATG TGATAAGTTG AGGAAAGATG GTTTCCGGAG CTCCCAGTAC 
TACTCTCAGG GCCCGACCTT CTCCGAGCCC TTACAGTCCA GCAGCAGCCT GCAAGACGAG 
GAAGATGAGG AAAATGACAA GAAGTCTTTA GCATCCTCGA TGGAGGATGA TAAATCCCAG 
CTCTCGCTGA GGTTGCAGGA GCGCTGCGGT GAAAGGGGCG AGATGTTGGA AGTTGACGGG 
CAGATGGTGT GGAATAAAGC GGTTCCCCTC CCGACGCCGG AGGAGAGGAT GAGGCAAGCA 
GCTCAGGCCG TGCCCACGGA CATAGTCCCC ATCAATGTCA CAGCTAAAGG ACGAGGCGGG 
GAACTTCGGC CACATTCCAT GTTCATCCCA GGACAGTACT CCACTTTGGG GAGAGTTGGG 
AGTGTCAACT CAACACTCCG CTTCGAGAAA AGAGACTCCA GCTGCCAGAC AGAAGAAGTG 
AAGATTGTCC CTCCATCTGT GAGAAGAATT CGAGCACAAA GAGGGCAGGG AATCGCCGCT 
CAAATGGCTG GGATATCTGC TTCCTCTTCT ACTGGAAGCA TCTCCATCTC GAGCACAGAT 
GGCTTGATTC TGCCCAATCA GTTTTTCAGA GACCCTTCCC GCTTTCACAG TCTGCCGCGA 
CAGGGTGCCA GAGTGTCCCT CAGTGCTGAT CCCATCTATA GCAGCACGCC CATTAAGTCA 
GAGGATCAGT TGCAGAGGAA TATTGGAAAA TTCCAGGTTG ACGAAACAGC GGTACACATC 
AGGAATGCCC CACGAACGAG CACCTTGCCC AGACCCACGT CTCAGGAGGT GAGGAGGACG 
CAGTCCAGCG AGTGGGGAGG TGGTCCAGCA TGTGTGGTCT CCCCTCAGGC AGCTTATTCC 
ACCTCCTTCA TTCCCAATGC CACCCTGTCC AACTCCACGG ATGTTATCAC CCTCAGCTCA 
TCTAGTCATT TATCCTTATC AGCTGTCTCA GCTTTGGCCA CTTCTACCCC CACGCGTGCA 
CCACAAGAGA ACGGTGTGAC GCCTTCAGGC CCCCCCAGTG AGTCGGGCCA CTTGGATGGA 
AGTGTTCATA GTCACAGTAC TTTGGCTCCC ACTTCACCAT CATGTCCTCC AGAAGAACAG 
TGGATCTATG ACTCACCCGA AAATGTGGTT CCACACCGCA CTCTGACCTC CAGCTGTTCC 
ACTCCCATCA ACCAGCTTTA TAGCAGCCTG GACCATTCCT CACGGACTGA TTCCAGCTCC 
CTCTACTCCC AGGACAACGA TGGATACTTC ACCTCTATGC ACATGGACTC AGGGCTGCGC 
TCCCGAAGTC ACGGAAGTGG GCACGGCGCA GGAGCTGGGC GGTCCCGACA CAGCATGTAT 
GAGTGTCGCG AGATGGCCAA TCGAGAAGAC TCTGGAAGCG TGTACAGCGA CCGCTCTCTG 
TCGCGCAGCA TCTCGCTCCG CAAGTCAAAG AAGCCACCGC CACCTCCGGC ACGCACAGAT 
TCTCTGAGGC GCAAGCCTGC CGCAAAAAAG CCCTTAGGTG GTGTTGGTCC AACAACTGTT 
GCCAATGGGG ACGGTGCTAA CATGCTCAAT GAAACGTTCC TTGCTAGCTT GCAGCAGAGC 
CTGCAAATGG GACTGAGGGG GTCAAAGGGA AAGGGTACCT CTCTATCTTC ACCCTCTCAC 
AGCCCCAGCA GTGACTATGA TGACCCTTGG TTGTTGCGAC CGCGCAGTCA GAGTAGCATC 
AGTGCAGGCA GCTCTGCCGC CTCATTAGCT AACACAAATG GTGTGTCTAA TGTCTACTCA 
TTATGCCATG TCACGCCCAC GCATAGTGAC ACCAGCAGTT TGCGTTCTGA CTATGCAGAG 
TCCTGGGGAT ACTACATGGA CTACCCTCGT AACCCTGGAG ACCAGGGGGG GCAGACCCCA 
CCAGCCCATG CTACAGATAA CATGTCAGCT GGGGGTCATC CAGGAGTCCT ACAGAATGGA 
GGAGAGATTC ACAGCAATAC TCAAACAGAT GGAGCTCCAG GCCAGGAGGG AGGGCAGTCA 
GGGAAGCCCA AACCATCCAG CTCCTCACCG GACAGGGTGC ACAGACTTAC CTCCCCGTCA 
AGCGGTTACT CCAGCCAGTC CAACACGCCT ACAGCCGGAA CCCCGGTGCC AGCCTTCGTC 
AGATCCATGT CTCCCTCCTC AGGGAGTCGG CCCAAACCCA AAGTGCCGGA GAGGAAGTCC 
TCGCTCCTCT CTTCGGGTTC TGTCTCTTCG TCCTCTACTT CTTTGTCCTC CAACACTTCA 
GACTCACTAA AGAGTTCAGG TCCTCCTCTG CCACCCCCTA ATCCCCTCCA TCTCACCTCT 
TCTACCCCAA ACACCCCTCT TGGTCCTCCT CCGCCCTTTC CTCCTCCTCT TCCTCCAAAT 
TCTACATCAG CTCCCTCATC TCCTCCTCCA CCTCCTCCAT TACCCAACTC TCCAAGAGAA 
ACCTTCTTGG CCCCTCTATC TGCGTCACCA GAATTCCCTC CTCCTCCATC TCCAGACCTG 
TTGATGCACC ACAGTTCGTC CTTAAATGGG AGCTTTAGTC CTCCACCTCC CCCTGAGACC 
GGGATCCCTC CACCACCTCC ACTGCCTGAC TTCACCCCAG CTTTTGGATC CCCAAATATT 
AGGAAATTGA CAAAAAATAC TCCTAAACCA CATCCTTCCA AACACTCCAC AGAGTCCCCC 
AAGCCCCTCA TCACCCCCTT CGCCCTGCAG AGTGTCCAGC TGCGCTCCGT CAAGCGATCA 
CAAGAGGAGA TCACTGGGGA GCTGGACCAA ATGAAACCTC AGGAAAATGG ACTGGACCTT 
CTCCAGGAGC TGAAACTCCA TAGTCTAGAT CCATCCCCCA TCCAGGAGCA CACCACTGTC 
ACGAGTAGCT CTCCAGACGA AGAGCTGCGG GAGTCCACAC CTTCGCCTGT GACGAAACTG 
CTGGAAGAGC TGAGTCTGGA CTGCAGTTTT ATAATCGACA CACAGGATAG TGGTGTCATC 
AATGGAAAAG CTGAGGACCA GAGTTACTGT CTATCAAATG GAAAAGACAA TGAGGAAGAG 
CTGCCACTGC CTAGCTGTCC AGAGAGATGT GGAAGCTCCT CCATCAAAGC CAAGCCTCCG 
CCAGTCTCCA AGAAACCCAA ACTTACCTTT GTTCCATCCC AACCAATCAG CGAGCCAGCT 
CAAACTCAAA ATGACAATGC AGCCAGTCTG TCGCCAGAGG ATCAGGTCGA TGCACAAACG 
ACAAATGACG TAACAAATAA GAGGAACAAC CGGGAGGAAG AGGCGTCAGA GAGCACAAAG 
GCTTCCACTG AGAACCTGGA CCAGAGCTGC CAAGATGCAG ATCTTGACCA CGACATTTGC 
ACTAATGGAG AGGTGTTTGA TGAGGATGAA GAAGAGGAAG AAGGAGAAGG AGCAAGCAGC 
ACCACGGGGT CCATCAGCTC AAAAGAGGAA GACACAAGTG ANGTTTTCGA ATCCAGACCG 
GCTGAACCTC CTTCACCCCA GTCGGCCGTA GGGGCAAGCG AGCAAAAAAT GGTGACCCCG 
ACTCCCTCCA AACCCCGAAC CACTGATGAC CTTTTTGCTG TCATACACAG GTCAAAGCGA 
AAGGTTCTCG GTCGCAGAGA GTCGGATGAA GACAAGTCCC GCCCCGGGGT TCAGGCACAG 
TCTCCACCCT CCACCCCGTC ACCCGTCTCT CCTGGGCCGG TTTCTCCGTC GGCCCGTCAG 
ATGGGTTCCA TCCAGCGAAA CCTACGCAAG TCCGCCACCA GCAGCGACAA CTTCAAGGCC 
CTCCTGCTTC GGAAGGGAAG CCGCTCTGAG AACAGTTTCC GCATGTCTGC TACTGAAATG 
CTGCGCTCCA CCGACCCGCG CTTCCAGCGA ACTCGCTCCG AGTCTGCGCT GGACCCCCCC 
ACTGCGTCTC CCACGTCACC GATGGCGCCT CACAGCCCCT CTGCCTCTTT TGGGCGGAGT 
AAGAGGGTAA CGGATGAGTG GAGCCGCTAC GATGCCCTGT CCTCGCCGAC ATATTCGCCT 
GGATCAAAGT TCGGGCGCTC TCGCGCGCCG CCTTCCGCCG CCAGCAGCAA ATACAACACT 
CGCAGCCGAA TCCTCAGCAG CCCGATGACT GTGATCTGTG AGCGTGAAGG GGAGCTGACT 
GAGAGCGAAT CTGGAGAGGT CGGCGAGAGC CGGGAAGGAG TCCAGACGCT TCCAGAAACC 
AACGGCACTT TATCCGAAGA GAGCAGAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714407.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570066.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OJa76384
Clone ID Related Accession (Same CDS sequence)	XM_020714407.1
Accession Version	XM_020714407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTA TCGGGACCTC GTTAAAGTCT ATAATTAAGT ACTTTAAAAG AAAGGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGANGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570066.1
CDS	143..4699
Translation	MVFIGTSLKSIIKYFKRKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714407.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714407.1

ORF Insert Sequence:

ATGGTGTTTA TCGGGACCTC GTTAAAGTCT ATAATTAAGT ACTTTAAAAG AAAGGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGANGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714408.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570067.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OJa76385
Clone ID Related Accession (Same CDS sequence)	XM_020714408.1
Accession Version	XM_020714408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TCCTGCATCC TCGATCACCT ACTGCGGCGT GGTTACAATC ACTCGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGANGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570067.1
CDS	19..4575
Translation	MEVVLHPRSPTAAWLQSLAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714408.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714408.1

ORF Insert Sequence:

ATGGAAGTAG TCCTGCATCC TCGATCACCT ACTGCGGCGT GGTTACAATC ACTCGCTGTG 
TCAAACCTGG ATGAGGAGAG CAAATGGACG GTACACTACA CGGCTCCTTG GCACCAGCAG 
GAAAACGTCT TCCTGCCGGG CAGCAGGCCG TCCTGCGTGG AGGACCTCCA CCGCCAAGCC 
AAAGTCAACC TCAAGACCAC CCTGCGAGAA TGTGATAAGT TGAGGAAAGA TGGTTTCCGG 
AGCTCCCAGT ACTACTCTCA GGGCCCGACC TTCTCCGAGC CCTTACAGTC CAGCAGCAGC 
CTGCAAGACG AGGAAGATGA GGAAAATGAC AAGAAGTCTT TAGCATCCTC GATGGAGGAT 
GATAAATCCC AGCTCTCGCT GAGGTTGCAG GAGCGCTGCG GTGAAAGGGG CGAGATGTTG 
GAAGTTGACG GGCAGATGGT GTGGAATAAA GCGGTTCCCC TCCCGACGCC GGAGGAGAGG 
ATGAGGCAAG CAGCTCAGGC CGTGCCCACG GACATAGTCC CCATCAATGT CACAGGGGCA 
GTGTTTGACC GCCAGGCGAG CATCCGGCGC TCCCTCATTA ACACTGATAC CGTGTCCCGT 
CGGCCCAAGA AGGTCAAACG CAGAAAGACT ATATCAGGGC TGCCTGACAA CTTCAACCAG 
GAGCTAGCTA AAGGACGAGG CGGGGAACTT CGGCCACATT CCATGTTCAT CCCAGGACAG 
TACTCCACTT TGGGGAGAGT TGGGAGTGTC AACTCAACAC TCCGCTTCGA GAAAAGAGAC 
TCCAGCTGCC AGACAGAAGA AGTGAAGATT GTCCCTCCAT CTGTGAGAAG AATTCGAGCA 
CAAAGAGGGC AGGGAATCGC CGCTCAAATG GCTGGGATAT CTGCTTCCTC TTCTACTGGA 
AGCATCTCCA TCTCGAGCAC AGATGGCTTG ATTCTGCCCA ATCAGTTTTT CAGAGACCCT 
TCCCGCTTTC ACAGTCTGCC GCGACAGGGT GCCAGAGTGT CCCTCAGTGC TGATCCCATC 
TATAGCAGCA CGCCCATTAA GTCAGAGGAT CAGTTGCAGA GGAATATTGG AAAATTCCAG 
GTTGACGAAA CAGCGGTACA CATCAGGAAT GCCCCACGAA CGAGCACCTT GCCCAGACCC 
ACGTCTCAGG AGGTGAGGAG GACGCAGTCC AGCGAGTGGG GAGGTGGTCC AGCATGTGTG 
GTCTCCCCTC AGGCAGCTTA TTCCACCTCC TTCATTCCCA ATGCCACCCT GTCCAACTCC 
ACGGATGTTA TCACCCTCAG CTCATCTAGT CATTTATCCT TATCAGCTGT CTCAGCTTTG 
GCCACTTCTA CCCCCACGCG TGCACCACAA GAGAACGGTG TGACGCCTTC AGGCCCCCCC 
AGTGAGTCGG GCCACTTGGA TGGAAGTGTT CATAGTCACA GTACTTTGGC TCCCACTTCA 
CCATCATGTC CTCCAGAAGA ACAGTGGATC TATGACTCAC CCGAAAATGT GGTTCCACAC 
CGCACTCTGA CCTCCAGCTG TTCCACTCCC ATCAACCAGC TTTATAGCAG CCTGGACCAT 
TCCTCACGGA CTGATTCCAG CTCCCTCTAC TCCCAGGACA ACGATGGATA CTTCACCTCT 
ATGCACATGG ACTCAGGGCT GCGCTCCCGA AGTCACGGAA GTGGGCACGG CGCAGGAGCT 
GGGCGGTCCC GACACAGCAT GTATGAGTGT CGCGAGATGG CCAATCGAGA AGACTCTGGA 
AGCGTGTACA GCGACCGCTC TCTGTCGCGC AGCATCTCGC TCCGCAAGTC AAAGAAGCCA 
CCGCCACCTC CGGCACGCAC AGATTCTCTG AGGCGCAAGC CTGCCGCAAA AAAGCCCTTA 
GGTGGTGTTG GTCCAACAAC TGTTGCCAAT GGGGACGGTG CTAACATGCT CAATGAAACG 
TTCCTTGCTA GCTTGCAGCA GAGCCTGCAA ATGGGACTGA GGGGGTCAAA GGGAAAGGGT 
ACCTCTCTAT CTTCACCCTC TCACAGCCCC AGCAGTGACT ATGATGACCC TTGGTTGTTG 
CGACCGCGCA GTCAGAGTAG CATCAGTGCA GGCAGCTCTG CCGCCTCATT AGCTAACACA 
AATGGTGTGT CTAATGTCTA CTCATTATGC CATGTCACGC CCACGCATAG TGACACCAGC 
AGTTTGCGTT CTGACTATGC AGAGTCCTGG GGATACTACA TGGACTACCC TCGTAACCCT 
GGAGACCAGG GGGGGCAGAC CCCACCAGCC CATGCTACAG ATAACATGTC AGCTGGGGGT 
CATCCAGGAG TCCTACAGAA TGGAGGAGAG ATTCACAGCA ATACTCAAAC AGATGGAGCT 
CCAGGCCAGG AGGGAGGGCA GTCAGGGAAG CCCAAACCAT CCAGCTCCTC ACCGGACAGG 
GTGCACAGAC TTACCTCCCC GTCAAGCGGT TACTCCAGCC AGTCCAACAC GCCTACAGCC 
GGAACCCCGG TGCCAGCCTT CGTCAGATCC ATGTCTCCCT CCTCAGGGAG TCGGCCCAAA 
CCCAAAGTGC CGGAGAGGAA GTCCTCGCTC CTCTCTTCGG GTTCTGTCTC TTCGTCCTCT 
ACTTCTTTGT CCTCCAACAC TTCAGACTCA CTAAAGAGTT CAGGTCCTCC TCTGCCACCC 
CCTAATCCCC TCCATCTCAC CTCTTCTACC CCAAACACCC CTCTTGGTCC TCCTCCGCCC 
TTTCCTCCTC CTCTTCCTCC AAATTCTACA TCAGCTCCCT CATCTCCTCC TCCACCTCCT 
CCATTACCCA ACTCTCCAAG AGAAACCTTC TTGGCCCCTC TATCTGCGTC ACCAGAATTC 
CCTCCTCCTC CATCTCCAGA CCTGTTGATG CACCACAGTT CGTCCTTAAA TGGGAGCTTT 
AGTCCTCCAC CTCCCCCTGA GACCGGGATC CCTCCACCAC CTCCACTGCC TGACTTCACC 
CCAGCTTTTG GATCCCCAAA TATTAGGAAA TTGACAAAAA ATACTCCTAA ACCACATCCT 
TCCAAACACT CCACAGAGTC CCCCAAGCCC CTCATCACCC CCTTCGCCCT GCAGAGTGTC 
CAGCTGCGCT CCGTCAAGCG ATCACAAGAG GAGATCACTG GGGAGCTGGA CCAAATGAAA 
CCTCAGGAAA ATGGACTGGA CCTTCTCCAG GAGCTGAAAC TCCATAGTCT AGATCCATCC 
CCCATCCAGG AGCACACCAC TGTCACGAGT AGCTCTCCAG ACGAAGAGCT GCGGGAGTCC 
ACACCTTCGC CTGTGACGAA ACTGCTGGAA GAGCTGAGTC TGGACTGCAG TTTTATAATC 
GACACACAGG ATAGTGGTGT CATCAATGGA AAAGCTGAGG ACCAGAGTTA CTGTCTATCA 
AATGGAAAAG ACAATGAGGA AGAGCTGCCA CTGCCTAGCT GTCCAGAGAG ATGTGGAAGC 
TCCTCCATCA AAGCCAAGCC TCCGCCAGTC TCCAAGAAAC CCAAACTTAC CTTTGTTCCA 
TCCCAACCAA TCAGCGAGCC AGCTCAAACT CAAAATGACA ATGCAGCCAG TCTGTCGCCA 
GAGGATCAGG TCGATGCACA AACGACAAAT GACGTAACAA ATAAGAGGAA CAACCGGGAG 
GAAGAGGCGT CAGAGAGCAC AAAGGCTTCC ACTGAGAACC TGGACCAGAG CTGCCAAGAT 
GCAGATCTTG ACCACGACAT TTGCACTAAT GGAGAGGTGT TTGATGAGGA TGAAGAAGAG 
GAAGAAGGAG AAGGAGCAAG CAGCACCACG GGGTCCATCA GCTCAAAAGA GGAAGACACA 
AGTGANGTTT TCGAATCCAG ACCGGCTGAA CCTCCTTCAC CCCAGTCGGC CGTAGGGGCA 
AGCGAGCAAA AAATGGTGAC CCCGACTCCC TCCAAACCCC GAACCACTGA TGACCTTTTT 
GCTGTCATAC ACAGGTCAAA GCGAAAGGTT CTCGGTCGCA GAGAGTCGGA TGAAGACAAG 
TCCCGCCCCG GGGTTCAGGC ACAGTCTCCA CCCTCCACCC CGTCACCCGT CTCTCCTGGG 
CCGGTTTCTC CGTCGGCCCG TCAGATGGGT TCCATCCAGC GAAACCTACG CAAGTCCGCC 
ACCAGCAGCG ACAACTTCAA GGCCCTCCTG CTTCGGAAGG GAAGCCGCTC TGAGAACAGT 
TTCCGCATGT CTGCTACTGA AATGCTGCGC TCCACCGACC CGCGCTTCCA GCGAACTCGC 
TCCGAGTCTG CGCTGGACCC CCCCACTGCG TCTCCCACGT CACCGATGGC GCCTCACAGC 
CCCTCTGCCT CTTTTGGGCG GAGTAAGAGG GTAACGGATG AGTGGAGCCG CTACGATGCC 
CTGTCCTCGC CGACATATTC GCCTGGATCA AAGTTCGGGC GCTCTCGCGC GCCGCCTTCC 
GCCGCCAGCA GCAAATACAA CACTCGCAGC CGAATCCTCA GCAGCCCGAT GACTGTGATC 
TGTGAGCGTG AAGGGGAGCT GACTGAGAGC GAATCTGGAG AGGTCGGCGA GAGCCGGGAA 
GGAGTCCAGA CGCTTCCAGA AACCAACGGC ACTTTATCCG AAGAGAGCAG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_020714409.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020570068.1 Oryzias latipes NHS like 1 (nhsl1), transcript variant X9, mRNA.

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CloneID	OJa76386
Clone ID Related Accession (Same CDS sequence)	XM_020714409.1
Accession Version	XM_020714409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTC TGAAAGCTGT GTCAAACCTG GATGAGGAGA GCAAATGGAC GGTACACTAC 
ACGGCTCCTT GGCACCAGCA GGAAAACGTC TTCCTGCCGG GCAGCAGGCC GTCCTGCGTG 
GAGGACCTCC ACCGCCAAGC CAAAGTCAAC CTCAAGACCA CCCTGCGAGA ATGTGATAAG 
TTGAGGAAAG ATGGTTTCCG GAGCTCCCAG TACTACTCTC AGGGCCCGAC CTTCTCCGAG 
CCCTTACAGT CCAGCAGCAG CCTGCAAGAC GAGGAAGATG AGGAAAATGA CAAGAAGTCT 
TTAGCATCCT CGATGGAGGA TGATAAATCC CAGCTCTCGC TGAGGTTGCA GGAGCGCTGC 
GGTGAAAGGG GCGAGATGTT GGAAGTTGAC GGGCAGATGG TGTGGAATAA AGCGGTTCCC 
CTCCCGACGC CGGAGGAGAG GATGAGGCAA GCAGCTCAGG CCGTGCCCAC GGACATAGTC 
CCCATCAATG TCACAGGGGC AGTGTTTGAC CGCCAGGCGA GCATCCGGCG CTCCCTCATT 
AACACTGATA CCGTGTCCCG TCGGCCCAAG AAGGTCAAAC GCAGAAAGAC TATATCAGGG 
CTGCCTGACA ACTTCAACCA GGAGCTAGCT AAAGGACGAG GCGGGGAACT TCGGCCACAT 
TCCATGTTCA TCCCAGGACA GTACTCCACT TTGGGGAGAG TTGGGAGTGT CAACTCAACA 
CTCCGCTTCG AGAAAAGAGA CTCCAGCTGC CAGACAGAAG AAGTGAAGAT TGTCCCTCCA 
TCTGTGAGAA GAATTCGAGC ACAAAGAGGG CAGGGAATCG CCGCTCAAAT GGCTGGGATA 
TCTGCTTCCT CTTCTACTGG AAGCATCTCC ATCTCGAGCA CAGATGGCTT GATTCTGCCC 
AATCAGTTTT TCAGAGACCC TTCCCGCTTT CACAGTCTGC CGCGACAGGG TGCCAGAGTG 
TCCCTCAGTG CTGATCCCAT CTATAGCAGC ACGCCCATTA AGTCAGAGGA TCAGTTGCAG 
AGGAATATTG GAAAATTCCA GGTTGACGAA ACAGCGGTAC ACATCAGGAA TGCCCCACGA 
ACGAGCACCT TGCCCAGACC CACGTCTCAG GAGGTGAGGA GGACGCAGTC CAGCGAGTGG 
GGAGGTGGTC CAGCATGTGT GGTCTCCCCT CAGGCAGCTT ATTCCACCTC CTTCATTCCC 
AATGCCACCC TGTCCAACTC CACGGATGTT ATCACCCTCA GCTCATCTAG TCATTTATCC 
TTATCAGCTG TCTCAGCTTT GGCCACTTCT ACCCCCACGC GTGCACCACA AGAGAACGGT 
GTGACGCCTT CAGGCCCCCC CAGTGAGTCG GGCCACTTGG ATGGAAGTGT TCATAGTCAC 
AGTACTTTGG CTCCCACTTC ACCATCATGT CCTCCAGAAG AACAGTGGAT CTATGACTCA 
CCCGAAAATG TGGTTCCACA CCGCACTCTG ACCTCCAGCT GTTCCACTCC CATCAACCAG 
CTTTATAGCA GCCTGGACCA TTCCTCACGG ACTGATTCCA GCTCCCTCTA CTCCCAGGAC 
AACGATGGAT ACTTCACCTC TATGCACATG GACTCAGGGC TGCGCTCCCG AAGTCACGGA 
AGTGGGCACG GCGCAGGAGC TGGGCGGTCC CGACACAGCA TGTATGAGTG TCGCGAGATG 
GCCAATCGAG AAGACTCTGG AAGCGTGTAC AGCGACCGCT CTCTGTCGCG CAGCATCTCG 
CTCCGCAAGT CAAAGAAGCC ACCGCCACCT CCGGCACGCA CAGATTCTCT GAGGCGCAAG 
CCTGCCGCAA AAAAGCCCTT AGGTGGTGTT GGTCCAACAA CTGTTGCCAA TGGGGACGGT 
GCTAACATGC TCAATGAAAC GTTCCTTGCT AGCTTGCAGC AGAGCCTGCA AATGGGACTG 
AGGGGGTCAA AGGGAAAGGG TACCTCTCTA TCTTCACCCT CTCACAGCCC CAGCAGTGAC 
TATGATGACC CTTGGTTGTT GCGACCGCGC AGTCAGAGTA GCATCAGTGC AGGCAGCTCT 
GCCGCCTCAT TAGCTAACAC AAATGGTGTG TCTAATGTCT ACTCATTATG CCATGTCACG 
CCCACGCATA GTGACACCAG CAGTTTGCGT TCTGACTATG CAGAGTCCTG GGGATACTAC 
ATGGACTACC CTCGTAACCC TGGAGACCAG GGGGGGCAGA CCCCACCAGC CCATGCTACA 
GATAACATGT CAGCTGGGGG TCATCCAGGA GTCCTACAGA ATGGAGGAGA GATTCACAGC 
AATACTCAAA CAGATGGAGC TCCAGGCCAG GAGGGAGGGC AGTCAGGGAA GCCCAAACCA 
TCCAGCTCCT CACCGGACAG GGTGCACAGA CTTACCTCCC CGTCAAGCGG TTACTCCAGC 
CAGTCCAACA CGCCTACAGC CGGAACCCCG GTGCCAGCCT TCGTCAGATC CATGTCTCCC 
TCCTCAGGGA GTCGGCCCAA ACCCAAAGTG CCGGAGAGGA AGTCCTCGCT CCTCTCTTCG 
GGTTCTGTCT CTTCGTCCTC TACTTCTTTG TCCTCCAACA CTTCAGACTC ACTAAAGAGT 
TCAGGTCCTC CTCTGCCACC CCCTAATCCC CTCCATCTCA CCTCTTCTAC CCCAAACACC 
CCTCTTGGTC CTCCTCCGCC CTTTCCTCCT CCTCTTCCTC CAAATTCTAC ATCAGCTCCC 
TCATCTCCTC CTCCACCTCC TCCATTACCC AACTCTCCAA GAGAAACCTT CTTGGCCCCT 
CTATCTGCGT CACCAGAATT CCCTCCTCCT CCATCTCCAG ACCTGTTGAT GCACCACAGT 
TCGTCCTTAA ATGGGAGCTT TAGTCCTCCA CCTCCCCCTG AGACCGGGAT CCCTCCACCA 
CCTCCACTGC CTGACTTCAC CCCAGCTTTT GGATCCCCAA ATATTAGGAA ATTGACAAAA 
AATACTCCTA AACCACATCC TTCCAAACAC TCCACAGAGT CCCCCAAGCC CCTCATCACC 
CCCTTCGCCC TGCAGAGTGT CCAGCTGCGC TCCGTCAAGC GATCACAAGA GGAGATCACT 
GGGGAGCTGG ACCAAATGAA ACCTCAGGAA AATGGACTGG ACCTTCTCCA GGAGCTGAAA 
CTCCATAGTC TAGATCCATC CCCCATCCAG GAGCACACCA CTGTCACGAG TAGCTCTCCA 
GACGAAGAGC TGCGGGAGTC CACACCTTCG CCTGTGACGA AACTGCTGGA AGAGCTGAGT 
CTGGACTGCA GTTTTATAAT CGACACACAG GATAGTGGTG TCATCAATGG AAAAGCTGAG 
GACCAGAGTT ACTGTCTATC AAATGGAAAA GACAATGAGG AAGAGCTGCC ACTGCCTAGC 
TGTCCAGAGA GATGTGGAAG CTCCTCCATC AAAGCCAAGC CTCCGCCAGT CTCCAAGAAA 
CCCAAACTTA CCTTTGTTCC ATCCCAACCA ATCAGCGAGC CAGCTCAAAC TCAAAATGAC 
AATGCAGCCA GTCTGTCGCC AGAGGATCAG GTCGATGCAC AAACGACAAA TGACGTAACA 
AATAAGAGGA ACAACCGGGA GGAAGAGGCG TCAGAGAGCA CAAAGGCTTC CACTGAGAAC 
CTGGACCAGA GCTGCCAAGA TGCAGATCTT GACCACGACA TTTGCACTAA TGGAGAGGTG 
TTTGATGAGG ATGAAGAAGA GGAAGAAGGA GAAGGAGCAA GCAGCACCAC GGGGTCCATC 
AGCTCAAAAG AGGAAGACAC AAGTGANGTT TTCGAATCCA GACCGGCTGA ACCTCCTTCA 
CCCCAGTCGG CCGTAGGGGC AAGCGAGCAA AAAATGGTGA CCCCGACTCC CTCCAAACCC 
CGAACCACTG ATGACCTTTT TGCTGTCATA CACAGGTCAA AGCGAAAGGT TCTCGGTCGC 
AGAGAGTCGG ATGAAGACAA GTCCCGCCCC GGGGTTCAGG CACAGTCTCC ACCCTCCACC 
CCGTCACCCG TCTCTCCTGG GCCGGTTTCT CCGTCGGCCC GTCAGATGGG TTCCATCCAG 
CGAAACCTAC GCAAGTCCGC CACCAGCAGC GACAACTTCA AGGCCCTCCT GCTTCGGAAG 
GGAAGCCGCT CTGAGAACAG TTTCCGCATG TCTGCTACTG AAATGCTGCG CTCCACCGAC 
CCGCGCTTCC AGCGAACTCG CTCCGAGTCT GCGCTGGACC CCCCCACTGC GTCTCCCACG 
TCACCGATGG CGCCTCACAG CCCCTCTGCC TCTTTTGGGC GGAGTAAGAG GGTAACGGAT 
GAGTGGAGCC GCTACGATGC CCTGTCCTCG CCGACATATT CGCCTGGATC AAAGTTCGGG 
CGCTCTCGCG CGCCGCCTTC CGCCGCCAGC AGCAAATACA ACACTCGCAG CCGAATCCTC 
AGCAGCCCGA TGACTGTGAT CTGTGAGCGT GAAGGGGAGC TGACTGAGAG CGAATCTGGA 
GAGGTCGGCG AGAGCCGGGA AGGAGTCCAG ACGCTTCCAG AAACCAACGG CACTTTATCC 
GAAGAGAGCA GAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020570068.1
CDS	139..4656
Translation	MFCLKAVSNLDEESKWTVHYTAPWHQQENVFLPGSRPSCVEDLHRQAKVNLKTTLRECDKLRKDGFRSSQYYSQGPTFSEPLQSSSSLQDEEDEENDKKSLASSMEDDKSQLSLRLQERCGERGEMLEVDGQMVWNKAVPLPTPEERMRQAAQAVPTDIVPINVTGAVFDRQASIRRSLINTDTVSRRPKKVKRRKTISGLPDNFNQELAKGRGGELRPHSMFIPGQYSTLGRVGSVNSTLRFEKRDSSCQTEEVKIVPPSVRRIRAQRGQGIAAQMAGISASSSTGSISISSTDGLILPNQFFRDPSRFHSLPRQGARVSLSADPIYSSTPIKSEDQLQRNIGKFQVDETAVHIRNAPRTSTLPRPTSQEVRRTQSSEWGGGPACVVSPQAAYSTSFIPNATLSNSTDVITLSSSSHLSLSAVSALATSTPTRAPQENGVTPSGPPSESGHLDGSVHSHSTLAPTSPSCPPEEQWIYDSPENVVPHRTLTSSCSTPINQLYSSLDHSSRTDSSSLYSQDNDGYFTSMHMDSGLRSRSHGSGHGAGAGRSRHSMYECREMANREDSGSVYSDRSLSRSISLRKSKKPPPPPARTDSLRRKPAAKKPLGGVGPTTVANGDGANMLNETFLASLQQSLQMGLRGSKGKGTSLSSPSHSPSSDYDDPWLLRPRSQSSISAGSSAASLANTNGVSNVYSLCHVTPTHSDTSSLRSDYAESWGYYMDYPRNPGDQGGQTPPAHATDNMSAGGHPGVLQNGGEIHSNTQTDGAPGQEGGQSGKPKPSSSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSSGSRPKPKVPERKSSLLSSGSVSSSSTSLSSNTSDSLKSSGPPLPPPNPLHLTSSTPNTPLGPPPPFPPPLPPNSTSAPSSPPPPPPLPNSPRETFLAPLSASPEFPPPPSPDLLMHHSSSLNGSFSPPPPPETGIPPPPPLPDFTPAFGSPNIRKLTKNTPKPHPSKHSTESPKPLITPFALQSVQLRSVKRSQEEITGELDQMKPQENGLDLLQELKLHSLDPSPIQEHTTVTSSSPDEELRESTPSPVTKLLEELSLDCSFIIDTQDSGVINGKAEDQSYCLSNGKDNEEELPLPSCPERCGSSSIKAKPPPVSKKPKLTFVPSQPISEPAQTQNDNAASLSPEDQVDAQTTNDVTNKRNNREEEASESTKASTENLDQSCQDADLDHDICTNGEVFDEDEEEEEGEGASSTTGSISSKEEDTSnullVFESRPAEPPSPQSAVGASEQKMVTPTPSKPRTTDDLFAVIHRSKRKVLGRRESDEDKSRPGVQAQSPPSTPSPVSPGPVSPSARQMGSIQRNLRKSATSSDNFKALLLRKGSRSENSFRMSATEMLRSTDPRFQRTRSESALDPPTASPTSPMAPHSPSASFGRSKRVTDEWSRYDALSSPTYSPGSKFGRSRAPPSAASSKYNTRSRILSSPMTVICEREGELTESESGEVGESREGVQTLPETNGTLSEESRG

Target ORF information:

RefSeq Version	XM_020714409.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NHS like 1 (nhsl1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020714409.1

ORF Insert Sequence:

ATGTTTTGTC TGAAAGCTGT GTCAAACCTG GATGAGGAGA GCAAATGGAC GGTACACTAC 
ACGGCTCCTT GGCACCAGCA GGAAAACGTC TTCCTGCCGG GCAGCAGGCC GTCCTGCGTG 
GAGGACCTCC ACCGCCAAGC CAAAGTCAAC CTCAAGACCA CCCTGCGAGA ATGTGATAAG 
TTGAGGAAAG ATGGTTTCCG GAGCTCCCAG TACTACTCTC AGGGCCCGAC CTTCTCCGAG 
CCCTTACAGT CCAGCAGCAG CCTGCAAGAC GAGGAAGATG AGGAAAATGA CAAGAAGTCT 
TTAGCATCCT CGATGGAGGA TGATAAATCC CAGCTCTCGC TGAGGTTGCA GGAGCGCTGC 
GGTGAAAGGG GCGAGATGTT GGAAGTTGAC GGGCAGATGG TGTGGAATAA AGCGGTTCCC 
CTCCCGACGC CGGAGGAGAG GATGAGGCAA GCAGCTCAGG CCGTGCCCAC GGACATAGTC 
CCCATCAATG TCACAGGGGC AGTGTTTGAC CGCCAGGCGA GCATCCGGCG CTCCCTCATT 
AACACTGATA CCGTGTCCCG TCGGCCCAAG AAGGTCAAAC GCAGAAAGAC TATATCAGGG 
CTGCCTGACA ACTTCAACCA GGAGCTAGCT AAAGGACGAG GCGGGGAACT TCGGCCACAT 
TCCATGTTCA TCCCAGGACA GTACTCCACT TTGGGGAGAG TTGGGAGTGT CAACTCAACA 
CTCCGCTTCG AGAAAAGAGA CTCCAGCTGC CAGACAGAAG AAGTGAAGAT TGTCCCTCCA 
TCTGTGAGAA GAATTCGAGC ACAAAGAGGG CAGGGAATCG CCGCTCAAAT GGCTGGGATA 
TCTGCTTCCT CTTCTACTGG AAGCATCTCC ATCTCGAGCA CAGATGGCTT GATTCTGCCC 
AATCAGTTTT TCAGAGACCC TTCCCGCTTT CACAGTCTGC CGCGACAGGG TGCCAGAGTG 
TCCCTCAGTG CTGATCCCAT CTATAGCAGC ACGCCCATTA AGTCAGAGGA TCAGTTGCAG 
AGGAATATTG GAAAATTCCA GGTTGACGAA ACAGCGGTAC ACATCAGGAA TGCCCCACGA 
ACGAGCACCT TGCCCAGACC CACGTCTCAG GAGGTGAGGA GGACGCAGTC CAGCGAGTGG 
GGAGGTGGTC CAGCATGTGT GGTCTCCCCT CAGGCAGCTT ATTCCACCTC CTTCATTCCC 
AATGCCACCC TGTCCAACTC CACGGATGTT ATCACCCTCA GCTCATCTAG TCATTTATCC 
TTATCAGCTG TCTCAGCTTT GGCCACTTCT ACCCCCACGC GTGCACCACA AGAGAACGGT 
GTGACGCCTT CAGGCCCCCC CAGTGAGTCG GGCCACTTGG ATGGAAGTGT TCATAGTCAC 
AGTACTTTGG CTCCCACTTC ACCATCATGT CCTCCAGAAG AACAGTGGAT CTATGACTCA 
CCCGAAAATG TGGTTCCACA CCGCACTCTG ACCTCCAGCT GTTCCACTCC CATCAACCAG 
CTTTATAGCA GCCTGGACCA TTCCTCACGG ACTGATTCCA GCTCCCTCTA CTCCCAGGAC 
AACGATGGAT ACTTCACCTC TATGCACATG GACTCAGGGC TGCGCTCCCG AAGTCACGGA 
AGTGGGCACG GCGCAGGAGC TGGGCGGTCC CGACACAGCA TGTATGAGTG TCGCGAGATG 
GCCAATCGAG AAGACTCTGG AAGCGTGTAC AGCGACCGCT CTCTGTCGCG CAGCATCTCG 
CTCCGCAAGT CAAAGAAGCC ACCGCCACCT CCGGCACGCA CAGATTCTCT GAGGCGCAAG 
CCTGCCGCAA AAAAGCCCTT AGGTGGTGTT GGTCCAACAA CTGTTGCCAA TGGGGACGGT 
GCTAACATGC TCAATGAAAC GTTCCTTGCT AGCTTGCAGC AGAGCCTGCA AATGGGACTG 
AGGGGGTCAA AGGGAAAGGG TACCTCTCTA TCTTCACCCT CTCACAGCCC CAGCAGTGAC 
TATGATGACC CTTGGTTGTT GCGACCGCGC AGTCAGAGTA GCATCAGTGC AGGCAGCTCT 
GCCGCCTCAT TAGCTAACAC AAATGGTGTG TCTAATGTCT ACTCATTATG CCATGTCACG 
CCCACGCATA GTGACACCAG CAGTTTGCGT TCTGACTATG CAGAGTCCTG GGGATACTAC 
ATGGACTACC CTCGTAACCC TGGAGACCAG GGGGGGCAGA CCCCACCAGC CCATGCTACA 
GATAACATGT CAGCTGGGGG TCATCCAGGA GTCCTACAGA ATGGAGGAGA GATTCACAGC 
AATACTCAAA CAGATGGAGC TCCAGGCCAG GAGGGAGGGC AGTCAGGGAA GCCCAAACCA 
TCCAGCTCCT CACCGGACAG GGTGCACAGA CTTACCTCCC CGTCAAGCGG TTACTCCAGC 
CAGTCCAACA CGCCTACAGC CGGAACCCCG GTGCCAGCCT TCGTCAGATC CATGTCTCCC 
TCCTCAGGGA GTCGGCCCAA ACCCAAAGTG CCGGAGAGGA AGTCCTCGCT CCTCTCTTCG 
GGTTCTGTCT CTTCGTCCTC TACTTCTTTG TCCTCCAACA CTTCAGACTC ACTAAAGAGT 
TCAGGTCCTC CTCTGCCACC CCCTAATCCC CTCCATCTCA CCTCTTCTAC CCCAAACACC 
CCTCTTGGTC CTCCTCCGCC CTTTCCTCCT CCTCTTCCTC CAAATTCTAC ATCAGCTCCC 
TCATCTCCTC CTCCACCTCC TCCATTACCC AACTCTCCAA GAGAAACCTT CTTGGCCCCT 
CTATCTGCGT CACCAGAATT CCCTCCTCCT CCATCTCCAG ACCTGTTGAT GCACCACAGT 
TCGTCCTTAA ATGGGAGCTT TAGTCCTCCA CCTCCCCCTG AGACCGGGAT CCCTCCACCA 
CCTCCACTGC CTGACTTCAC CCCAGCTTTT GGATCCCCAA ATATTAGGAA ATTGACAAAA 
AATACTCCTA AACCACATCC TTCCAAACAC TCCACAGAGT CCCCCAAGCC CCTCATCACC 
CCCTTCGCCC TGCAGAGTGT CCAGCTGCGC TCCGTCAAGC GATCACAAGA GGAGATCACT 
GGGGAGCTGG ACCAAATGAA ACCTCAGGAA AATGGACTGG ACCTTCTCCA GGAGCTGAAA 
CTCCATAGTC TAGATCCATC CCCCATCCAG GAGCACACCA CTGTCACGAG TAGCTCTCCA 
GACGAAGAGC TGCGGGAGTC CACACCTTCG CCTGTGACGA AACTGCTGGA AGAGCTGAGT 
CTGGACTGCA GTTTTATAAT CGACACACAG GATAGTGGTG TCATCAATGG AAAAGCTGAG 
GACCAGAGTT ACTGTCTATC AAATGGAAAA GACAATGAGG AAGAGCTGCC ACTGCCTAGC 
TGTCCAGAGA GATGTGGAAG CTCCTCCATC AAAGCCAAGC CTCCGCCAGT CTCCAAGAAA 
CCCAAACTTA CCTTTGTTCC ATCCCAACCA ATCAGCGAGC CAGCTCAAAC TCAAAATGAC 
AATGCAGCCA GTCTGTCGCC AGAGGATCAG GTCGATGCAC AAACGACAAA TGACGTAACA 
AATAAGAGGA ACAACCGGGA GGAAGAGGCG TCAGAGAGCA CAAAGGCTTC CACTGAGAAC 
CTGGACCAGA GCTGCCAAGA TGCAGATCTT GACCACGACA TTTGCACTAA TGGAGAGGTG 
TTTGATGAGG ATGAAGAAGA GGAAGAAGGA GAAGGAGCAA GCAGCACCAC GGGGTCCATC 
AGCTCAAAAG AGGAAGACAC AAGTGANGTT TTCGAATCCA GACCGGCTGA ACCTCCTTCA 
CCCCAGTCGG CCGTAGGGGC AAGCGAGCAA AAAATGGTGA CCCCGACTCC CTCCAAACCC 
CGAACCACTG ATGACCTTTT TGCTGTCATA CACAGGTCAA AGCGAAAGGT TCTCGGTCGC 
AGAGAGTCGG ATGAAGACAA GTCCCGCCCC GGGGTTCAGG CACAGTCTCC ACCCTCCACC 
CCGTCACCCG TCTCTCCTGG GCCGGTTTCT CCGTCGGCCC GTCAGATGGG TTCCATCCAG 
CGAAACCTAC GCAAGTCCGC CACCAGCAGC GACAACTTCA AGGCCCTCCT GCTTCGGAAG 
GGAAGCCGCT CTGAGAACAG TTTCCGCATG TCTGCTACTG AAATGCTGCG CTCCACCGAC 
CCGCGCTTCC AGCGAACTCG CTCCGAGTCT GCGCTGGACC CCCCCACTGC GTCTCCCACG 
TCACCGATGG CGCCTCACAG CCCCTCTGCC TCTTTTGGGC GGAGTAAGAG GGTAACGGAT 
GAGTGGAGCC GCTACGATGC CCTGTCCTCG CCGACATATT CGCCTGGATC AAAGTTCGGG 
CGCTCTCGCG CGCCGCCTTC CGCCGCCAGC AGCAAATACA ACACTCGCAG CCGAATCCTC 
AGCAGCCCGA TGACTGTGAT CTGTGAGCGT GAAGGGGAGC TGACTGAGAG CGAATCTGGA 
GAGGTCGGCG AGAGCCGGGA AGGAGTCCAG ACGCTTCCAG AAACCAACGG CACTTTATCC 
GAAGAGAGCA GAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nhsl1
Entrez Gene ID	101162889
Full Name	NHS like 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nhsl1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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