vars cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	vars
Entrez Gene ID	101158795
Full Name	valyl-tRNA synthetase
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004078001.2	XP_004078049.1	valine--tRNA ligase
XM_004078001.3	XP_004078049.1	valine--tRNA ligase

Pathways from BioSystems
Gene Ontology

The following vars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa12276	XM_004078001.3 Latest version!	Oryzias latipes valyl-tRNA synthetase (vars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OJa12276	XM_004078001.2	Oryzias latipes valyl-tRNA synthetase (vars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vars ( XM_004078001.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004078049.1 Oryzias latipes valyl-tRNA synthetase (vars), mRNA.

Price & Availability↑

CloneID	OJa12276
Clone ID Related Accession (Same CDS sequence)	XM_004078001.2 , XM_004078001.3
Accession Version	XM_004078001.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_004078001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC TCTACGTGTC TCCTCATCCT GATCATTTCA GGAGTCTTCT GGCTCTCATA 
GCTGCAGAGT TTTCTCCTTC ATCCCGTCCA CAGATCATCA CAGAGGACCC ACCTTCCTCC 
CTGAATGTCC ACTCAAGACC AGCCCTTAGA CTCGGTACTG GTGTGAGCGA CTCTGTCCTG 
TGTGGCGGGT CTGCCGTGGC CTGGTACCTG GCTTCTCAGG GGAAGAGGGC AGGTGTCGAT 
CTGAAGCAGC AGAGCCAGGT GTGGCAGTGG CTCAGCTTTG CAGAAAATGA GCTCACCCCA 
TTGTCTTGTG CTGTAGCATT CCCTTTGATG GGAGTCACGG GAGTCGATAA AAAGATTCAG 
CAGAACTCCC GGGCAGAGCT GATGGGTATT CTAAACGTTC TTGATCAGGC GCTTGCACCT 
AGAACCTTCT TGGTGGGAGA AAGCATCACT CTTGCAGATA TGGCTGTAGC TACAGCTGTT 
CTCCTGCCTT TTAAATATGC TTTGGAGCCC TCAGACAGGA AGTTGATAAT CAATGTAACC 
AGATGGTTCA CAACCTGCAT TAATCAGCCC CAGTTCCTGG CGGTGCTTGG GGAGATACCT 
CTGTGCGAAA GGGCTGTGTC TGTAACGCCA AACACCAATG CTGCTGCAAA AGCCAAAAAT 
GCACAGACTA CTCCAGGTGT GGACTCAGCT GCTGCTGCTG CTGCTGTTGT TGCTGCTGCT 
ACTAATGGCC CACCAAAGAC GGAGGCGCAG CTAAAAAAGG AGGCAAAGAA GAGAGAAAAG 
CTTGAAAAGT TTCAACAGAA GAAAGAAATG GAGGCAAAGA AAAAGACACA ACCAGCAGCA 
GAGAAAAAAG TTAAACCAGA AAAGAAAGAG CTTGGAGTGA TAACATACAA CATCCCAACC 
CCTGCTGGAG AAAAAAAAGA TGTTGTCGGT TCTCTTCCTG ACTCCTACAG TCCTCAGTAT 
GTGGAGGCTG CCTGGTACAC ATGGTGGGAG AAGCAGGGCT TCTTCAAACC TGAGTATGGG 
AGGAAAAGTG TTGGTGAGAA GAACCCTCGC GGCATCTTCA TGATGTGCAT CCCTCCTCCT 
AACGTGACAG GATCCCTTCA TTTGGGTCAC GCCCTCACCA ACGCCATTCA GGACTGTTTG 
ACCCGATGGC ACAGGATGCG AGGAGAGACC ACCCTGTGGA ACCCGGGCTG TGACCATGCT 
GGCATCGCCA CGCAGGTGGT TGTGGAGAAG AAGCTGATGA GAGAACGAGG TTTGAGCCGC 
CACGATCTGG GGAGAGAGAA CTTCATTCAG GAGGTCTGGA AATGGAAGAA TGAAAAGGGG 
GACCGCATTT ACCATCAGCT GAAAAAACTG GGCTCCTCTC TGGACTGGGA CAGAGCGTGC 
TTCACTATGG ATCCGAAACT GTCCTATGCA GTGCAGGAGG CCTTCATCCG CATGCACGAA 
GATGGAGTGA TTTACCGGAG CAAAAGGCTG GTGAACTGGT CCTGCACTCT GAACTCTGCC 
ATCTCTGACA TAGAGGTGGA TAAAAAGGAG CTTTCTGGCA GGACTCTGCT GCCTGTGCCC 
GGTTACAAAG ATAAAGTGGA GTTTGGGGTC TTGGTGTCTT TTGCCTACAA AGTGGAGGGA 
TCAGATGAGG AGGTGATTGT GGCGACAACC CGTATCGAGA CCATGTTGGG AGACACGGCC 
GTGGCCGTCC ACCCTGCCGA CCCCAGATAC CAGCATCTGA AGGGGAAAAT GGTGCTGCAT 
CCCTTCTGCG ATCGGAAGAT GCCAATAGTC TTGGATGACT TTGTGGACAT GAGCTTTGGA 
ACAGGTGCCG TCAAAATCAC CCCAGCACAT GACCATAACG ATTATGAGGT TGGAGAGAGG 
CACAGCCTGG CCTTCATCAA CATCTTGGAT GAGAACGGCC TGCTCATCAA TGTGCCCCCT 
CCCTTCCTGG GCATGAAACG TTTTGAGGCC AGAAAAGCGG TGCTGCAGGC TCTCAAGGAC 
AGAGGCCACT TTAAAGAGAT CAAAGACAAC CCCATGGTCG TCCCAGTTTG CAGTCGCTCT 
AAAGACATCG TGGAGCCTCT GCTGAAGCCG CAGTGGTACG TGAGCTGCAG AGACATGGGC 
AGGGAGGCTG CGGACGCCGT CAGAGAGGGA AGGCTCAAAA TCATCCCTGA TCACCACCTC 
AAGACCTGGT TCAACTGGAT GGACAACATC AGAGACTGGT GCATCTCCCG ACAGCTGTGG 
TGGGGTCACC GCATTCCCGC CTATTTCGTC ACCGTCAGCG ATCCTTCTGT GAAAGCTGGA 
GAGGACATGG ACGGCCATTA CTGGGTAAGC GGACGGTCCG AGGTGGAGGC CAGGGAAAAA 
GCGGCAAAAC GCTTCAACGT GTCTGCGGAC AAAATAACTC TCAGACAGGA CGAGGATGTT 
CTGGACACTT GGTTCTCGTC TGGCATTTTT CCTTTCTCCA TCTTCGGTTG GCCTAATGAG 
ACTGAAGACC TGAATGTTTT CTACCCCGGC ACCCTGCTAG AAACGGGCCA TGACATCTTG 
TTCTTCTGGG TTGCCCGCAT GGTGATGATG GGCCTCAAAC TGACAGGCAA GCTGCCATTT 
AAGGAGGTTT ATCTGCACGC GGTGGTGCGG GATGCTCACG GAAGGAAGAT GAGCAAGTCT 
CTGGGCAACG TCATTGACCC GCTTGATGTC ATTACAGGGA TCTCCCTTGA GGGTCTCCAT 
GCTCAACTGA CGGACAGCAA CTTGGATCCT GTGGAGGTGG AAAAGGCAAA GCAGGGACAG 
AAGTCAGACT ACCCAAATGG CATCCCAGAG TGTGGGACAG ATGCTCTCCG CTTTGCCTTA 
TGCGCCTACA CCAGTCAAGG AAGAGATATC AACCTTGATG TAAATCGTAT CTTGGGCTAC 
CGTCACTTCT GCAATAAACT ATGGAACGCT GTGAAGTTTG CCATGAAGAC ACTAGGAGAC 
ACCTTTGTCC CGTTAGAGAA GGCACAGCTG TCGGGGGAGG AAAGTGTATC CGACAGGTGG 
ATCTTATCCA GGCTGAGTGC TGCTGTGGCG CTCTGTGACG CTGGCTTCAA GGCATACGAC 
TTCCCAGCCA TCACCACTGC CGTCTACAAC TTCTGGCTCT ACGAGCTCTG CGATGTCTAT 
CTGGAAAGTG TTAAACCCGT GTTCAGTAAA GCAGAGGAAG ATGGGGACCG CCAGAGAGAA 
GCCATGGTAT GCAGACAGAC CCTGTACACC TGTCTAGAGG TGGGTCTCCG CCTTCTGTCT 
CCTTTGATGC CCTTCGTCAC TGAAGAACTG TACCAGAGGT TACCACGCCG ACAGCCTCAG 
ACTGACCCGC CCAGCATCTG TGTGACGGCA TACCCAGACT CAAAAGAGTT CTGCTGGAAC 
AGCGAGGAAG TTGACCTCAA CATGGAGTTC ATCATGACTG TGGTCAGAAC GATCCGATCG 
CTCAGAGCTG ACTACAATCT GACGAAGACT AAAGCTGACT GCTACCTTCA GTGCATAGAC 
TCTGCTACTG TGTCCCTGGT GCAGAAATAC AGTCTACAGA TCCAGACCTT GTCTTACTCT 
CAGGCCGTCA TCCCCCTGAC AGCCAGCGAG CCCGTCCCAC AAGGCTGCGC AGTGGCTATC 
GCTTCTGACA GATGCACCGT CAACCTTATG CTCAAGGGTC TTATTGATGT GGAAAAGGAA 
GTGACAAAGC TGCTGACAAA GAAAGGCGAC TTGGAGAAAC AGCTGGAGAA ACTGCAAGAG 
AAGATGGCAA AGAGTGATTA CAAGGAGAAG GTTCCAGTGA AGGTGCAGGA GCAGGATGCT 
GAGAAGCTAC GACAGAGCCA GGCTGAACTT GAGAAAGTCA AAGAAGCCAT AGACAATTTT 
AGGAAAATGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004078049.1
CDS	118..3972
Translation	MATLYVSPHPDHFRSLLALIAAEFSPSSRPQIITEDPPSSLNVHSRPALRLGTGVSDSVLCGGSAVAWYLASQGKRAGVDLKQQSQVWQWLSFAENELTPLSCAVAFPLMGVTGVDKKIQQNSRAELMGILNVLDQALAPRTFLVGESITLADMAVATAVLLPFKYALEPSDRKLIINVTRWFTTCINQPQFLAVLGEIPLCERAVSVTPNTNAAAKAKNAQTTPGVDSAAAAAAVVAAATNGPPKTEAQLKKEAKKREKLEKFQQKKEMEAKKKTQPAAEKKVKPEKKELGVITYNIPTPAGEKKDVVGSLPDSYSPQYVEAAWYTWWEKQGFFKPEYGRKSVGEKNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERGLSRHDLGRENFIQEVWKWKNEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFIRMHEDGVIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKDKVEFGVLVSFAYKVEGSDEEVIVATTRIETMLGDTAVAVHPADPRYQHLKGKMVLHPFCDRKMPIVLDDFVDMSFGTGAVKITPAHDHNDYEVGERHSLAFINILDENGLLINVPPPFLGMKRFEARKAVLQALKDRGHFKEIKDNPMVVPVCSRSKDIVEPLLKPQWYVSCRDMGREAADAVREGRLKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFVTVSDPSVKAGEDMDGHYWVSGRSEVEAREKAAKRFNVSADKITLRQDEDVLDTWFSSGIFPFSIFGWPNETEDLNVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLHAQLTDSNLDPVEVEKAKQGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFAMKTLGDTFVPLEKAQLSGEESVSDRWILSRLSAAVALCDAGFKAYDFPAITTAVYNFWLYELCDVYLESVKPVFSKAEEDGDRQREAMVCRQTLYTCLEVGLRLLSPLMPFVTEELYQRLPRRQPQTDPPSICVTAYPDSKEFCWNSEEVDLNMEFIMTVVRTIRSLRADYNLTKTKADCYLQCIDSATVSLVQKYSLQIQTLSYSQAVIPLTASEPVPQGCAVAIASDRCTVNLMLKGLIDVEKEVTKLLTKKGDLEKQLEKLQEKMAKSDYKEKVPVKVQEQDAEKLRQSQAELEKVKEAIDNFRKMM

Target ORF information:

RefSeq Version	XM_004078001.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes valyl-tRNA synthetase (vars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004078001.2

ORF Insert Sequence:

ATGGCCACCC TCTACGTGTC TCCTCATCCT GATCATTTCA GGAGTCTTCT GGCTCTCATA 
GCTGCAGAGT TTTCTCCTTC ATCCCGTCCA CAGATCATCA CAGAGGACCC ACCTTCCTCC 
CTGAATGTCC ACTCAAGACC AGCCCTTAGA CTCGGTACTG GTGTGAGCGA CTCTGTCCTG 
TGTGGCGGGT CTGCCGTGGC CTGGTACCTG GCTTCTCAGG GGAAGAGGGC AGGTGTCGAT 
CTGAAGCAGC AGAGCCAGGT GTGGCAGTGG CTCAGCTTTG CAGAAAATGA GCTCACCCCA 
TTGTCTTGTG CTGTAGCATT CCCTTTGATG GGAGTCACGG GAGTCGATAA AAAGATTCAG 
CAGAACTCCC GGGCAGAGCT GATGGGTATT CTAAACGTTC TTGATCAGGC GCTTGCACCT 
AGAACCTTCT TGGTGGGAGA AAGCATCACT CTTGCAGATA TGGCTGTAGC TACAGCTGTT 
CTCCTGCCTT TTAAATATGC TTTGGAGCCC TCAGACAGGA AGTTGATAAT CAATGTAACC 
AGATGGTTCA CAACCTGCAT TAATCAGCCC CAGTTCCTGG CGGTGCTTGG GGAGATACCT 
CTGTGCGAAA GGGCTGTGTC TGTAACGCCA AACACCAATG CTGCTGCAAA AGCCAAAAAT 
GCACAGACTA CTCCAGGTGT GGACTCAGCT GCTGCTGCTG CTGCTGTTGT TGCTGCTGCT 
ACTAATGGCC CACCAAAGAC GGAGGCGCAG CTAAAAAAGG AGGCAAAGAA GAGAGAAAAG 
CTTGAAAAGT TTCAACAGAA GAAAGAAATG GAGGCAAAGA AAAAGACACA ACCAGCAGCA 
GAGAAAAAAG TTAAACCAGA AAAGAAAGAG CTTGGAGTGA TAACATACAA CATCCCAACC 
CCTGCTGGAG AAAAAAAAGA TGTTGTCGGT TCTCTTCCTG ACTCCTACAG TCCTCAGTAT 
GTGGAGGCTG CCTGGTACAC ATGGTGGGAG AAGCAGGGCT TCTTCAAACC TGAGTATGGG 
AGGAAAAGTG TTGGTGAGAA GAACCCTCGC GGCATCTTCA TGATGTGCAT CCCTCCTCCT 
AACGTGACAG GATCCCTTCA TTTGGGTCAC GCCCTCACCA ACGCCATTCA GGACTGTTTG 
ACCCGATGGC ACAGGATGCG AGGAGAGACC ACCCTGTGGA ACCCGGGCTG TGACCATGCT 
GGCATCGCCA CGCAGGTGGT TGTGGAGAAG AAGCTGATGA GAGAACGAGG TTTGAGCCGC 
CACGATCTGG GGAGAGAGAA CTTCATTCAG GAGGTCTGGA AATGGAAGAA TGAAAAGGGG 
GACCGCATTT ACCATCAGCT GAAAAAACTG GGCTCCTCTC TGGACTGGGA CAGAGCGTGC 
TTCACTATGG ATCCGAAACT GTCCTATGCA GTGCAGGAGG CCTTCATCCG CATGCACGAA 
GATGGAGTGA TTTACCGGAG CAAAAGGCTG GTGAACTGGT CCTGCACTCT GAACTCTGCC 
ATCTCTGACA TAGAGGTGGA TAAAAAGGAG CTTTCTGGCA GGACTCTGCT GCCTGTGCCC 
GGTTACAAAG ATAAAGTGGA GTTTGGGGTC TTGGTGTCTT TTGCCTACAA AGTGGAGGGA 
TCAGATGAGG AGGTGATTGT GGCGACAACC CGTATCGAGA CCATGTTGGG AGACACGGCC 
GTGGCCGTCC ACCCTGCCGA CCCCAGATAC CAGCATCTGA AGGGGAAAAT GGTGCTGCAT 
CCCTTCTGCG ATCGGAAGAT GCCAATAGTC TTGGATGACT TTGTGGACAT GAGCTTTGGA 
ACAGGTGCCG TCAAAATCAC CCCAGCACAT GACCATAACG ATTATGAGGT TGGAGAGAGG 
CACAGCCTGG CCTTCATCAA CATCTTGGAT GAGAACGGCC TGCTCATCAA TGTGCCCCCT 
CCCTTCCTGG GCATGAAACG TTTTGAGGCC AGAAAAGCGG TGCTGCAGGC TCTCAAGGAC 
AGAGGCCACT TTAAAGAGAT CAAAGACAAC CCCATGGTCG TCCCAGTTTG CAGTCGCTCT 
AAAGACATCG TGGAGCCTCT GCTGAAGCCG CAGTGGTACG TGAGCTGCAG AGACATGGGC 
AGGGAGGCTG CGGACGCCGT CAGAGAGGGA AGGCTCAAAA TCATCCCTGA TCACCACCTC 
AAGACCTGGT TCAACTGGAT GGACAACATC AGAGACTGGT GCATCTCCCG ACAGCTGTGG 
TGGGGTCACC GCATTCCCGC CTATTTCGTC ACCGTCAGCG ATCCTTCTGT GAAAGCTGGA 
GAGGACATGG ACGGCCATTA CTGGGTAAGC GGACGGTCCG AGGTGGAGGC CAGGGAAAAA 
GCGGCAAAAC GCTTCAACGT GTCTGCGGAC AAAATAACTC TCAGACAGGA CGAGGATGTT 
CTGGACACTT GGTTCTCGTC TGGCATTTTT CCTTTCTCCA TCTTCGGTTG GCCTAATGAG 
ACTGAAGACC TGAATGTTTT CTACCCCGGC ACCCTGCTAG AAACGGGCCA TGACATCTTG 
TTCTTCTGGG TTGCCCGCAT GGTGATGATG GGCCTCAAAC TGACAGGCAA GCTGCCATTT 
AAGGAGGTTT ATCTGCACGC GGTGGTGCGG GATGCTCACG GAAGGAAGAT GAGCAAGTCT 
CTGGGCAACG TCATTGACCC GCTTGATGTC ATTACAGGGA TCTCCCTTGA GGGTCTCCAT 
GCTCAACTGA CGGACAGCAA CTTGGATCCT GTGGAGGTGG AAAAGGCAAA GCAGGGACAG 
AAGTCAGACT ACCCAAATGG CATCCCAGAG TGTGGGACAG ATGCTCTCCG CTTTGCCTTA 
TGCGCCTACA CCAGTCAAGG AAGAGATATC AACCTTGATG TAAATCGTAT CTTGGGCTAC 
CGTCACTTCT GCAATAAACT ATGGAACGCT GTGAAGTTTG CCATGAAGAC ACTAGGAGAC 
ACCTTTGTCC CGTTAGAGAA GGCACAGCTG TCGGGGGAGG AAAGTGTATC CGACAGGTGG 
ATCTTATCCA GGCTGAGTGC TGCTGTGGCG CTCTGTGACG CTGGCTTCAA GGCATACGAC 
TTCCCAGCCA TCACCACTGC CGTCTACAAC TTCTGGCTCT ACGAGCTCTG CGATGTCTAT 
CTGGAAAGTG TTAAACCCGT GTTCAGTAAA GCAGAGGAAG ATGGGGACCG CCAGAGAGAA 
GCCATGGTAT GCAGACAGAC CCTGTACACC TGTCTAGAGG TGGGTCTCCG CCTTCTGTCT 
CCTTTGATGC CCTTCGTCAC TGAAGAACTG TACCAGAGGT TACCACGCCG ACAGCCTCAG 
ACTGACCCGC CCAGCATCTG TGTGACGGCA TACCCAGACT CAAAAGAGTT CTGCTGGAAC 
AGCGAGGAAG TTGACCTCAA CATGGAGTTC ATCATGACTG TGGTCAGAAC GATCCGATCG 
CTCAGAGCTG ACTACAATCT GACGAAGACT AAAGCTGACT GCTACCTTCA GTGCATAGAC 
TCTGCTACTG TGTCCCTGGT GCAGAAATAC AGTCTACAGA TCCAGACCTT GTCTTACTCT 
CAGGCCGTCA TCCCCCTGAC AGCCAGCGAG CCCGTCCCAC AAGGCTGCGC AGTGGCTATC 
GCTTCTGACA GATGCACCGT CAACCTTATG CTCAAGGGTC TTATTGATGT GGAAAAGGAA 
GTGACAAAGC TGCTGACAAA GAAAGGCGAC TTGGAGAAAC AGCTGGAGAA ACTGCAAGAG 
AAGATGGCAA AGAGTGATTA CAAGGAGAAG GTTCCAGTGA AGGTGCAGGA GCAGGATGCT 
GAGAAGCTAC GACAGAGCCA GGCTGAACTT GAGAAAGTCA AAGAAGCCAT AGACAATTTT 
AGGAAAATGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars ( XM_004078001.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004078049.1 Oryzias latipes valyl-tRNA synthetase (vars), mRNA.

Price & Availability↑

CloneID	OJa12276
Clone ID Related Accession (Same CDS sequence)	XM_004078001.2 , XM_004078001.3
Accession Version	XM_004078001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_004078001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC TCTACGTGTC TCCTCATCCT GATCATTTCA GGAGTCTTCT GGCTCTCATA 
GCTGCAGAGT TTTCTCCTTC ATCCCGTCCA CAGATCATCA CAGAGGACCC ACCTTCCTCC 
CTGAATGTCC ACTCAAGACC AGCCCTTAGA CTCGGTACTG GTGTGAGCGA CTCTGTCCTG 
TGTGGCGGGT CTGCCGTGGC CTGGTACCTG GCTTCTCAGG GGAAGAGGGC AGGTGTCGAT 
CTGAAGCAGC AGAGCCAGGT GTGGCAGTGG CTCAGCTTTG CAGAAAATGA GCTCACCCCA 
TTGTCTTGTG CTGTAGCATT CCCTTTGATG GGAGTCACGG GAGTCGATAA AAAGATTCAG 
CAGAACTCCC GGGCAGAGCT GATGGGTATT CTAAACGTTC TTGATCAGGC GCTTGCACCT 
AGAACCTTCT TGGTGGGAGA AAGCATCACT CTTGCAGATA TGGCTGTAGC TACAGCTGTT 
CTCCTGCCTT TTAAATATGC TTTGGAGCCC TCAGACAGGA AGTTGATAAT CAATGTAACC 
AGATGGTTCA CAACCTGCAT TAATCAGCCC CAGTTCCTGG CGGTGCTTGG GGAGATACCT 
CTGTGCGAAA GGGCTGTGTC TGTAACGCCA AACACCAATG CTGCTGCAAA AGCCAAAAAT 
GCACAGACTA CTCCAGGTGT GGACTCAGCT GCTGCTGCTG CTGCTGTTGT TGCTGCTGCT 
ACTAATGGCC CACCAAAGAC GGAGGCGCAG CTAAAAAAGG AGGCAAAGAA GAGAGAAAAG 
CTTGAAAAGT TTCAACAGAA GAAAGAAATG GAGGCAAAGA AAAAGACACA ACCAGCAGCA 
GAGAAAAAAG TTAAACCAGA AAAGAAAGAG CTTGGAGTGA TAACATACAA CATCCCAACC 
CCTGCTGGAG AAAAAAAAGA TGTTGTCGGT TCTCTTCCTG ACTCCTACAG TCCTCAGTAT 
GTGGAGGCTG CCTGGTACAC ATGGTGGGAG AAGCAGGGCT TCTTCAAACC TGAGTATGGG 
AGGAAAAGTG TTGGTGAGAA GAACCCTCGC GGCATCTTCA TGATGTGCAT CCCTCCTCCT 
AACGTGACAG GATCCCTTCA TTTGGGTCAC GCCCTCACCA ACGCCATTCA GGACTGTTTG 
ACCCGATGGC ACAGGATGCG AGGAGAGACC ACCCTGTGGA ACCCGGGCTG TGACCATGCT 
GGCATCGCCA CGCAGGTGGT TGTGGAGAAG AAGCTGATGA GAGAACGAGG TTTGAGCCGC 
CACGATCTGG GGAGAGAGAA CTTCATTCAG GAGGTCTGGA AATGGAAGAA TGAAAAGGGG 
GACCGCATTT ACCATCAGCT GAAAAAACTG GGCTCCTCTC TGGACTGGGA CAGAGCGTGC 
TTCACTATGG ATCCGAAACT GTCCTATGCA GTGCAGGAGG CCTTCATCCG CATGCACGAA 
GATGGAGTGA TTTACCGGAG CAAAAGGCTG GTGAACTGGT CCTGCACTCT GAACTCTGCC 
ATCTCTGACA TAGAGGTGGA TAAAAAGGAG CTTTCTGGCA GGACTCTGCT GCCTGTGCCC 
GGTTACAAAG ATAAAGTGGA GTTTGGGGTC TTGGTGTCTT TTGCCTACAA AGTGGAGGGA 
TCAGATGAGG AGGTGATTGT GGCGACAACC CGTATCGAGA CCATGTTGGG AGACACGGCC 
GTGGCCGTCC ACCCTGCCGA CCCCAGATAC CAGCATCTGA AGGGGAAAAT GGTGCTGCAT 
CCCTTCTGCG ATCGGAAGAT GCCAATAGTC TTGGATGACT TTGTGGACAT GAGCTTTGGA 
ACAGGTGCCG TCAAAATCAC CCCAGCACAT GACCATAACG ATTATGAGGT TGGAGAGAGG 
CACAGCCTGG CCTTCATCAA CATCTTGGAT GAGAACGGCC TGCTCATCAA TGTGCCCCCT 
CCCTTCCTGG GCATGAAACG TTTTGAGGCC AGAAAAGCGG TGCTGCAGGC TCTCAAGGAC 
AGAGGCCACT TTAAAGAGAT CAAAGACAAC CCCATGGTCG TCCCAGTTTG CAGTCGCTCT 
AAAGACATCG TGGAGCCTCT GCTGAAGCCG CAGTGGTACG TGAGCTGCAG AGACATGGGC 
AGGGAGGCTG CGGACGCCGT CAGAGAGGGA AGGCTCAAAA TCATCCCTGA TCACCACCTC 
AAGACCTGGT TCAACTGGAT GGACAACATC AGAGACTGGT GCATCTCCCG ACAGCTGTGG 
TGGGGTCACC GCATTCCCGC CTATTTCGTC ACCGTCAGCG ATCCTTCTGT GAAAGCTGGA 
GAGGACATGG ACGGCCATTA CTGGGTAAGC GGACGGTCCG AGGTGGAGGC CAGGGAAAAA 
GCGGCAAAAC GCTTCAACGT GTCTGCGGAC AAAATAACTC TCAGACAGGA CGAGGATGTT 
CTGGACACTT GGTTCTCGTC TGGCATTTTT CCTTTCTCCA TCTTCGGTTG GCCTAATGAG 
ACTGAAGACC TGAATGTTTT CTACCCCGGC ACCCTGCTAG AAACGGGCCA TGACATCTTG 
TTCTTCTGGG TTGCCCGCAT GGTGATGATG GGCCTCAAAC TGACAGGCAA GCTGCCATTT 
AAGGAGGTTT ATCTGCACGC GGTGGTGCGG GATGCTCACG GAAGGAAGAT GAGCAAGTCT 
CTGGGCAACG TCATTGACCC GCTTGATGTC ATTACAGGGA TCTCCCTTGA GGGTCTCCAT 
GCTCAACTGA CGGACAGCAA CTTGGATCCT GTGGAGGTGG AAAAGGCAAA GCAGGGACAG 
AAGTCAGACT ACCCAAATGG CATCCCAGAG TGTGGGACAG ATGCTCTCCG CTTTGCCTTA 
TGCGCCTACA CCAGTCAAGG AAGAGATATC AACCTTGATG TAAATCGTAT CTTGGGCTAC 
CGTCACTTCT GCAATAAACT ATGGAACGCT GTGAAGTTTG CCATGAAGAC ACTAGGAGAC 
ACCTTTGTCC CGTTAGAGAA GGCACAGCTG TCGGGGGAGG AAAGTGTATC CGACAGGTGG 
ATCTTATCCA GGCTGAGTGC TGCTGTGGCG CTCTGTGACG CTGGCTTCAA GGCATACGAC 
TTCCCAGCCA TCACCACTGC CGTCTACAAC TTCTGGCTCT ACGAGCTCTG CGATGTCTAT 
CTGGAAAGTG TTAAACCCGT GTTCAGTAAA GCAGAGGAAG ATGGGGACCG CCAGAGAGAA 
GCCATGGTAT GCAGACAGAC CCTGTACACC TGTCTAGAGG TGGGTCTCCG CCTTCTGTCT 
CCTTTGATGC CCTTCGTCAC TGAAGAACTG TACCAGAGGT TACCACGCCG ACAGCCTCAG 
ACTGACCCGC CCAGCATCTG TGTGACGGCA TACCCAGACT CAAAAGAGTT CTGCTGGAAC 
AGCGAGGAAG TTGACCTCAA CATGGAGTTC ATCATGACTG TGGTCAGAAC GATCCGATCG 
CTCAGAGCTG ACTACAATCT GACGAAGACT AAAGCTGACT GCTACCTTCA GTGCATAGAC 
TCTGCTACTG TGTCCCTGGT GCAGAAATAC AGTCTACAGA TCCAGACCTT GTCTTACTCT 
CAGGCCGTCA TCCCCCTGAC AGCCAGCGAG CCCGTCCCAC AAGGCTGCGC AGTGGCTATC 
GCTTCTGACA GATGCACCGT CAACCTTATG CTCAAGGGTC TTATTGATGT GGAAAAGGAA 
GTGACAAAGC TGCTGACAAA GAAAGGCGAC TTGGAGAAAC AGCTGGAGAA ACTGCAAGAG 
AAGATGGCAA AGAGTGATTA CAAGGAGAAG GTTCCAGTGA AGGTGCAGGA GCAGGATGCT 
GAGAAGCTAC GACAGAGCCA GGCTGAACTT GAGAAAGTCA AAGAAGCCAT AGACAATTTT 
AGGAAAATGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004078049.1
CDS	110..3964
Translation	MATLYVSPHPDHFRSLLALIAAEFSPSSRPQIITEDPPSSLNVHSRPALRLGTGVSDSVLCGGSAVAWYLASQGKRAGVDLKQQSQVWQWLSFAENELTPLSCAVAFPLMGVTGVDKKIQQNSRAELMGILNVLDQALAPRTFLVGESITLADMAVATAVLLPFKYALEPSDRKLIINVTRWFTTCINQPQFLAVLGEIPLCERAVSVTPNTNAAAKAKNAQTTPGVDSAAAAAAVVAAATNGPPKTEAQLKKEAKKREKLEKFQQKKEMEAKKKTQPAAEKKVKPEKKELGVITYNIPTPAGEKKDVVGSLPDSYSPQYVEAAWYTWWEKQGFFKPEYGRKSVGEKNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERGLSRHDLGRENFIQEVWKWKNEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFIRMHEDGVIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKDKVEFGVLVSFAYKVEGSDEEVIVATTRIETMLGDTAVAVHPADPRYQHLKGKMVLHPFCDRKMPIVLDDFVDMSFGTGAVKITPAHDHNDYEVGERHSLAFINILDENGLLINVPPPFLGMKRFEARKAVLQALKDRGHFKEIKDNPMVVPVCSRSKDIVEPLLKPQWYVSCRDMGREAADAVREGRLKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFVTVSDPSVKAGEDMDGHYWVSGRSEVEAREKAAKRFNVSADKITLRQDEDVLDTWFSSGIFPFSIFGWPNETEDLNVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLHAQLTDSNLDPVEVEKAKQGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFAMKTLGDTFVPLEKAQLSGEESVSDRWILSRLSAAVALCDAGFKAYDFPAITTAVYNFWLYELCDVYLESVKPVFSKAEEDGDRQREAMVCRQTLYTCLEVGLRLLSPLMPFVTEELYQRLPRRQPQTDPPSICVTAYPDSKEFCWNSEEVDLNMEFIMTVVRTIRSLRADYNLTKTKADCYLQCIDSATVSLVQKYSLQIQTLSYSQAVIPLTASEPVPQGCAVAIASDRCTVNLMLKGLIDVEKEVTKLLTKKGDLEKQLEKLQEKMAKSDYKEKVPVKVQEQDAEKLRQSQAELEKVKEAIDNFRKMM

Target ORF information:

RefSeq Version	XM_004078001.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes valyl-tRNA synthetase (vars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004078001.3

ORF Insert Sequence:

ATGGCCACCC TCTACGTGTC TCCTCATCCT GATCATTTCA GGAGTCTTCT GGCTCTCATA 
GCTGCAGAGT TTTCTCCTTC ATCCCGTCCA CAGATCATCA CAGAGGACCC ACCTTCCTCC 
CTGAATGTCC ACTCAAGACC AGCCCTTAGA CTCGGTACTG GTGTGAGCGA CTCTGTCCTG 
TGTGGCGGGT CTGCCGTGGC CTGGTACCTG GCTTCTCAGG GGAAGAGGGC AGGTGTCGAT 
CTGAAGCAGC AGAGCCAGGT GTGGCAGTGG CTCAGCTTTG CAGAAAATGA GCTCACCCCA 
TTGTCTTGTG CTGTAGCATT CCCTTTGATG GGAGTCACGG GAGTCGATAA AAAGATTCAG 
CAGAACTCCC GGGCAGAGCT GATGGGTATT CTAAACGTTC TTGATCAGGC GCTTGCACCT 
AGAACCTTCT TGGTGGGAGA AAGCATCACT CTTGCAGATA TGGCTGTAGC TACAGCTGTT 
CTCCTGCCTT TTAAATATGC TTTGGAGCCC TCAGACAGGA AGTTGATAAT CAATGTAACC 
AGATGGTTCA CAACCTGCAT TAATCAGCCC CAGTTCCTGG CGGTGCTTGG GGAGATACCT 
CTGTGCGAAA GGGCTGTGTC TGTAACGCCA AACACCAATG CTGCTGCAAA AGCCAAAAAT 
GCACAGACTA CTCCAGGTGT GGACTCAGCT GCTGCTGCTG CTGCTGTTGT TGCTGCTGCT 
ACTAATGGCC CACCAAAGAC GGAGGCGCAG CTAAAAAAGG AGGCAAAGAA GAGAGAAAAG 
CTTGAAAAGT TTCAACAGAA GAAAGAAATG GAGGCAAAGA AAAAGACACA ACCAGCAGCA 
GAGAAAAAAG TTAAACCAGA AAAGAAAGAG CTTGGAGTGA TAACATACAA CATCCCAACC 
CCTGCTGGAG AAAAAAAAGA TGTTGTCGGT TCTCTTCCTG ACTCCTACAG TCCTCAGTAT 
GTGGAGGCTG CCTGGTACAC ATGGTGGGAG AAGCAGGGCT TCTTCAAACC TGAGTATGGG 
AGGAAAAGTG TTGGTGAGAA GAACCCTCGC GGCATCTTCA TGATGTGCAT CCCTCCTCCT 
AACGTGACAG GATCCCTTCA TTTGGGTCAC GCCCTCACCA ACGCCATTCA GGACTGTTTG 
ACCCGATGGC ACAGGATGCG AGGAGAGACC ACCCTGTGGA ACCCGGGCTG TGACCATGCT 
GGCATCGCCA CGCAGGTGGT TGTGGAGAAG AAGCTGATGA GAGAACGAGG TTTGAGCCGC 
CACGATCTGG GGAGAGAGAA CTTCATTCAG GAGGTCTGGA AATGGAAGAA TGAAAAGGGG 
GACCGCATTT ACCATCAGCT GAAAAAACTG GGCTCCTCTC TGGACTGGGA CAGAGCGTGC 
TTCACTATGG ATCCGAAACT GTCCTATGCA GTGCAGGAGG CCTTCATCCG CATGCACGAA 
GATGGAGTGA TTTACCGGAG CAAAAGGCTG GTGAACTGGT CCTGCACTCT GAACTCTGCC 
ATCTCTGACA TAGAGGTGGA TAAAAAGGAG CTTTCTGGCA GGACTCTGCT GCCTGTGCCC 
GGTTACAAAG ATAAAGTGGA GTTTGGGGTC TTGGTGTCTT TTGCCTACAA AGTGGAGGGA 
TCAGATGAGG AGGTGATTGT GGCGACAACC CGTATCGAGA CCATGTTGGG AGACACGGCC 
GTGGCCGTCC ACCCTGCCGA CCCCAGATAC CAGCATCTGA AGGGGAAAAT GGTGCTGCAT 
CCCTTCTGCG ATCGGAAGAT GCCAATAGTC TTGGATGACT TTGTGGACAT GAGCTTTGGA 
ACAGGTGCCG TCAAAATCAC CCCAGCACAT GACCATAACG ATTATGAGGT TGGAGAGAGG 
CACAGCCTGG CCTTCATCAA CATCTTGGAT GAGAACGGCC TGCTCATCAA TGTGCCCCCT 
CCCTTCCTGG GCATGAAACG TTTTGAGGCC AGAAAAGCGG TGCTGCAGGC TCTCAAGGAC 
AGAGGCCACT TTAAAGAGAT CAAAGACAAC CCCATGGTCG TCCCAGTTTG CAGTCGCTCT 
AAAGACATCG TGGAGCCTCT GCTGAAGCCG CAGTGGTACG TGAGCTGCAG AGACATGGGC 
AGGGAGGCTG CGGACGCCGT CAGAGAGGGA AGGCTCAAAA TCATCCCTGA TCACCACCTC 
AAGACCTGGT TCAACTGGAT GGACAACATC AGAGACTGGT GCATCTCCCG ACAGCTGTGG 
TGGGGTCACC GCATTCCCGC CTATTTCGTC ACCGTCAGCG ATCCTTCTGT GAAAGCTGGA 
GAGGACATGG ACGGCCATTA CTGGGTAAGC GGACGGTCCG AGGTGGAGGC CAGGGAAAAA 
GCGGCAAAAC GCTTCAACGT GTCTGCGGAC AAAATAACTC TCAGACAGGA CGAGGATGTT 
CTGGACACTT GGTTCTCGTC TGGCATTTTT CCTTTCTCCA TCTTCGGTTG GCCTAATGAG 
ACTGAAGACC TGAATGTTTT CTACCCCGGC ACCCTGCTAG AAACGGGCCA TGACATCTTG 
TTCTTCTGGG TTGCCCGCAT GGTGATGATG GGCCTCAAAC TGACAGGCAA GCTGCCATTT 
AAGGAGGTTT ATCTGCACGC GGTGGTGCGG GATGCTCACG GAAGGAAGAT GAGCAAGTCT 
CTGGGCAACG TCATTGACCC GCTTGATGTC ATTACAGGGA TCTCCCTTGA GGGTCTCCAT 
GCTCAACTGA CGGACAGCAA CTTGGATCCT GTGGAGGTGG AAAAGGCAAA GCAGGGACAG 
AAGTCAGACT ACCCAAATGG CATCCCAGAG TGTGGGACAG ATGCTCTCCG CTTTGCCTTA 
TGCGCCTACA CCAGTCAAGG AAGAGATATC AACCTTGATG TAAATCGTAT CTTGGGCTAC 
CGTCACTTCT GCAATAAACT ATGGAACGCT GTGAAGTTTG CCATGAAGAC ACTAGGAGAC 
ACCTTTGTCC CGTTAGAGAA GGCACAGCTG TCGGGGGAGG AAAGTGTATC CGACAGGTGG 
ATCTTATCCA GGCTGAGTGC TGCTGTGGCG CTCTGTGACG CTGGCTTCAA GGCATACGAC 
TTCCCAGCCA TCACCACTGC CGTCTACAAC TTCTGGCTCT ACGAGCTCTG CGATGTCTAT 
CTGGAAAGTG TTAAACCCGT GTTCAGTAAA GCAGAGGAAG ATGGGGACCG CCAGAGAGAA 
GCCATGGTAT GCAGACAGAC CCTGTACACC TGTCTAGAGG TGGGTCTCCG CCTTCTGTCT 
CCTTTGATGC CCTTCGTCAC TGAAGAACTG TACCAGAGGT TACCACGCCG ACAGCCTCAG 
ACTGACCCGC CCAGCATCTG TGTGACGGCA TACCCAGACT CAAAAGAGTT CTGCTGGAAC 
AGCGAGGAAG TTGACCTCAA CATGGAGTTC ATCATGACTG TGGTCAGAAC GATCCGATCG 
CTCAGAGCTG ACTACAATCT GACGAAGACT AAAGCTGACT GCTACCTTCA GTGCATAGAC 
TCTGCTACTG TGTCCCTGGT GCAGAAATAC AGTCTACAGA TCCAGACCTT GTCTTACTCT 
CAGGCCGTCA TCCCCCTGAC AGCCAGCGAG CCCGTCCCAC AAGGCTGCGC AGTGGCTATC 
GCTTCTGACA GATGCACCGT CAACCTTATG CTCAAGGGTC TTATTGATGT GGAAAAGGAA 
GTGACAAAGC TGCTGACAAA GAAAGGCGAC TTGGAGAAAC AGCTGGAGAA ACTGCAAGAG 
AAGATGGCAA AGAGTGATTA CAAGGAGAAG GTTCCAGTGA AGGTGCAGGA GCAGGATGCT 
GAGAAGCTAC GACAGAGCCA GGCTGAACTT GAGAAAGTCA AAGAAGCCAT AGACAATTTT 
AGGAAAATGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vars cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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