COL27A1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL27A1
Entrez Gene ID	100078516
Full Name	collagen alpha-1(XXVII) chain-like
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016226379.1	XP_016081865.1	collagen alpha-1(XXVII) chain-like
XM_029062842.1	XP_028918675.1	collagen alpha-1(XXVII) chain isoform X1
XM_029062843.1	XP_028918676.1	collagen alpha-1(XXVII) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following COL27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc38018	XM_016226379.1 Latest version!	Ornithorhynchus anatinus collagen alpha-1(XXVII) chain-like (LOC100078516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOc127552	XM_029062842.1 Latest version!	Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc127553	XM_029062843.1 Latest version!	Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL27A1 ( XM_016226379.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_016081865.1 Ornithorhynchus anatinus collagen alpha-1(XXVII) chain-like (LOC100078516), mRNA.

Price & Availability↑

CloneID	OOc38018
Clone ID Related Accession (Same CDS sequence)	XM_016226379.1
Accession Version	XM_016226379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-1(XXVII) chain-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009097.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 35% of CDS bases ##RefSeq-Attributes-END##

ATGGTGAATG TGTATTCCGA CCTGGGATGG GATTTTTCCG GCGCCCCGGA CCTGGGTATC 
AGGATCGTGA AGTCAGGCCT CCTGCAAGAA GTTAATTCAG TGAATTCTTA TGAATTTGCC 
ATCCTTGACT ATCTGAAAGT GGGAATCTTC TTTCTCTGGA TTGTCTTCTG TTCTACCTAT 
TGTCTGGATT TCACCCAGGG AGTTCCTGAA GATGTGGATG TCCTCCAACA GCTGGGCCTT 
GGCAGGAAGC CAAAGGCAGG TGGAAGTCCC TTGGCATCCC ACCCTGTCCC CCAGGGGGTC 
ATCCCGTTCC AATCAGGAGT CATCTTCACC CAGCGGGCCC GGGTGGACGC CCCCACTGGC 
ACGGTCATCC CTACTGCTTT TGGCACCGAC CTGGCCCTGG TCCTGAGCCT CCGCTCCCAC 
CGAGTCAACA ATGCCTTCCT GTTCGCCATC AAGAGCGGGA GGAAGAAGCT GCAGCTGGGA 
GTGCAGTTCA TCCCCGGCAA GATCGCCGTC TATGTGGGCC AACGGCGCTC TGTGCACTTT 
GACTACGAGG TCCACGATGG CCAGTGGCAT CACCTGGCCA TTGATATCCG GGGCCGAGCG 
GTCACCTTAT TTACTTCTTG TGGGAAGCGC CGAGTACATG CAGATCTGCA TTTTAAAAAA 
GACACGGCAC TAGACCCCGA GGGGTCGTTC CTCCTCGGGA AAATGAACCA GCACTCGACC 
CCATTCGAGG GCGCCGTGCG CCAGTTTGAT ATCTATCCCT CTGCCCGGGC AGCTCCTAAT 
TACTGGGCCA GCACCCTCCA CCCCACCGTC AGACCGGTGC TCCCTACCTC TGCACCCCAA 
ACTCTCCTGA GCCCTCCTAC CCCCGACTGG GGCCTGCCTG CCCGCCGGGG CCCCACCAGC 
TCCAAGAAGA AGGTGACAGG GCAGAAGGCT GCCAAGAAAG CCCACCTAGA GACGACCCCC 
AAACGAGTCC CTCCCACCAA GCCACGGCAG AAGAAGACCC CAGCAGACGC CCCAGCCAGC 
GACTACCTGC TCTTTGATCC CATCGGGCCG ACCCTCTTCC CGTTCCTGCT GGGACCCCCA 
GGCCAGAAAG GAGACACCGG AGCTGTGGGT TCTCCTGGCC CTCACGGAAA CCCTGGTCGC 
CCCGGCCCCC CTGGTGCTAA AGGCCACAAA GGGGATCCTG GAAGGTCACC TGGAAAGGCC 
TACAATGGGG AAAAGGGAAA TGCAGGCTTA CCTGGATTGA CAGGGCAGCT GGGACCAGTT 
GGTCGGAAGG GGCAGAAGGG CTACCCGGGA CCATCAGGGC ATCCCGGAGA CCAGGGGGAG 
CCAGGACCAG CGGGAAGTCC GGGTGCCAAA GGTTATCCTG GCAGGCAGGG ATTACCTGGA 
GCCATAGGAG ACCCAGGACC CAAAGGCAGC CGGGTAAGTG GTGCTTTGCC GGTGTTTTGG 
GTTCAGCCAG TTGGGCAGCA GGGCCGAGGT GTACCCGGAG TGCCTGGCCA GAGGGGCAAG 
ATGGGTAGGC CGGGCTTCCC AGGAGACTTC GGGGAAAGAG GACCCCCTGG AACGGATGGC 
AACCCCGGTG AACTGGGTGC CCCAGGCCCC CCTGGTGTTC CTGGGCTCAT TGGTGACATG 
GGATTGTTGG GGCCAATTGG GTACCCGGGA CCCAAAGGAT TGAAGGGACT GATGGGAAAC 
TTCGGAGAAC ATGGACTGAA AGGTGATAAG GGTGACCAAG GGCTACCAGG GGTTCCCGGA 
GATATCGGAT TCCAAGGAGA CAAGGGTAGC CAGGGCATGG TGGGGGCACC GGGTGTTCGA 
GGGAAGCCAG GGCCTCCTGG TAAGTCTGGT GACAAAGGAC CTGCTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC GGGAGACATC GGACCCCCAG GTGAAAACGG TCCAGAAGGC 
ACCAAGGGTA AGCCAGGAGC CCGAGGTTTA CCGGGACCAA GAGGACAGTC CGGCCAGGAG 
GGAGATGAAG GACCATCGGG GCCCCCGGGA TCACCTGGCC CAGAGGGCCA CCCTGGACGA 
AAAGGGTTTC CCGGAAGGCC TGGACCCAAC GGCCTAAAGG GGGAGCCAGG AAATCCAGGC 
CGACCTGGAA AAGTAGGGGA ACAGGGTTTG ATGGGATTTA TCGGTCTTGT AGGGGAGCCA 
GGAATCGTGG GAGAGAAGGG GGCCCGAGGG CCAGACGGAC CGACCGGGGA GCCGGGGTCG 
CGGGGTGTGA AGCTCAGTAT CAGGGTCTTC AAGCAAAAAG TTCCCTCCCG ACCCAGCTTC 
TCTCAAAGTC CCCCAGCCCT GGACCAGGCT CCCAGGACCC GAGGCGGCCT GCTCAGGGGA 
CCGCAGGGAA CCCAGGGCAA ACAGGGTCTA ACCGGACAGC AGGGTATCCA GGGTCCATCC 
GGCCCTCCCG GGGCTAAGGG AATTCCTGGG GAACCGGTGA AAGCAGTAGA AAGTGATTTT 
GTTTCAGATA GCTTCCTGTC ATGCTACAGT ATTGATTCCA CCACCAAAAG GCTGAATTTG 
CTAGGACAGT ATCTCAAGGC CAGAGGGCCC TCCCGGTGCA TTAGGAGAGC CTGGCCCTCC 
TGGGGAACCA GGGATGAAGT TCCCAGCCCC CTGGTCCGGT TGGCCCTACT CTTCCTGCTG 
GCCCAGGTCC TCCAGCCTCC CGTCCTGGGC TGTGAGCCGC AGCCTCGGAC CCTGGACAAC 
GTCACTGTCT CCAAGCTGTT CGGCCAGTGG TACTTTGTAG CTGGGGCATC TTCGCTGCCC 
AGGCACCGGC TGGAGATGCG GCTCATCGAT AGCGGGGTGT TGCAGGTGTC CCCCTCGGGG 
AGCCAGGACC GCCTGGACAT CACCCAGCAC CTGAGACTGG GGAACACATG TCTCTCTGAC 
AACAGCAGCT CCATCAAGAT CACTCAGGAC TACACCACGG GCTCTTCCCA TTCTGATCTG 
CTGGTGTTGG ACCAGGGAGG GGAGATCTTT AAGACCCTAC ACTACCTCAG CAACCTCATC 
CAGAGCATCA AGAAGCCACT GGGCACCAAG GAGAACCCTG CTCGCATCTG TCGAGACCTC 
ATGAACTGTG AGCAGAAGAT GGCTGACGGC AAATACTGGA TCGACCCCAA CCTTGGCTGT 
TCTTCAGACA CCATCGAAGT GACCTGTAAC TTCACCAAAG GAGGTCAAAC CTGTCTAAAA 
CCCATCACTG CCTCAAAGGT ACAGAGTTGC ATGGTGCCAG GGGAAAGCCC AAATTGTGCA 
ATGGTGTTAG TGGTGGTGGT GGTAGTTGGA GGAGATGCAG GAGCAAGAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016081865.1
CDS	1..3294
Translation	MVNVYSDLGWDFSGAPDLGIRIVKSGLLQEVNSVNSYEFAILDYLKVGIFFLWIVFCSTYCLDFTQGVPEDVDVLQQLGLGRKPKAGGSPLASHPVPQGVIPFQSGVIFTQRARVDAPTGTVIPTAFGTDLALVLSLRSHRVNNAFLFAIKSGRKKLQLGVQFIPGKIAVYVGQRRSVHFDYEVHDGQWHHLAIDIRGRAVTLFTSCGKRRVHADLHFKKDTALDPEGSFLLGKMNQHSTPFEGAVRQFDIYPSARAAPNYWASTLHPTVRPVLPTSAPQTLLSPPTPDWGLPARRGPTSSKKKVTGQKAAKKAHLETTPKRVPPTKPRQKKTPADAPASDYLLFDPIGPTLFPFLLGPPGQKGDTGAVGSPGPHGNPGRPGPPGAKGHKGDPGRSPGKAYNGEKGNAGLPGLTGQLGPVGRKGQKGYPGPSGHPGDQGEPGPAGSPGAKGYPGRQGLPGAIGDPGPKGSRVSGALPVFWVQPVGQQGRGVPGVPGQRGKMGRPGFPGDFGERGPPGTDGNPGELGAPGPPGVPGLIGDMGLLGPIGYPGPKGLKGLMGNFGEHGLKGDKGDQGLPGVPGDIGFQGDKGSQGMVGAPGVRGKPGPPGKSGDKGPAGFPGPPGPEGFPGDIGPPGENGPEGTKGKPGARGLPGPRGQSGQEGDEGPSGPPGSPGPEGHPGRKGFPGRPGPNGLKGEPGNPGRPGKVGEQGLMGFIGLVGEPGIVGEKGARGPDGPTGEPGSRGVKLSIRVFKQKVPSRPSFSQSPPALDQAPRTRGGLLRGPQGTQGKQGLTGQQGIQGPSGPPGAKGIPGEPVKAVESDFVSDSFLSCYSIDSTTKRLNLLGQYLKARGPSRCIRRAWPSWGTRDEVPSPLVRLALLFLLAQVLQPPVLGCEPQPRTLDNVTVSKLFGQWYFVAGASSLPRHRLEMRLIDSGVLQVSPSGSQDRLDITQHLRLGNTCLSDNSSSIKITQDYTTGSSHSDLLVLDQGGEIFKTLHYLSNLIQSIKKPLGTKENPARICRDLMNCEQKMADGKYWIDPNLGCSSDTIEVTCNFTKGGQTCLKPITASKVQSCMVPGESPNCAMVLVVVVVVGGDAGARA

Target ORF information:

RefSeq Version	XM_016226379.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen alpha-1(XXVII) chain-like (LOC100078516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016226379.1

ORF Insert Sequence:

ATGGTGAATG TGTATTCCGA CCTGGGATGG GATTTTTCCG GCGCCCCGGA CCTGGGTATC 
AGGATCGTGA AGTCAGGCCT CCTGCAAGAA GTTAATTCAG TGAATTCTTA TGAATTTGCC 
ATCCTTGACT ATCTGAAAGT GGGAATCTTC TTTCTCTGGA TTGTCTTCTG TTCTACCTAT 
TGTCTGGATT TCACCCAGGG AGTTCCTGAA GATGTGGATG TCCTCCAACA GCTGGGCCTT 
GGCAGGAAGC CAAAGGCAGG TGGAAGTCCC TTGGCATCCC ACCCTGTCCC CCAGGGGGTC 
ATCCCGTTCC AATCAGGAGT CATCTTCACC CAGCGGGCCC GGGTGGACGC CCCCACTGGC 
ACGGTCATCC CTACTGCTTT TGGCACCGAC CTGGCCCTGG TCCTGAGCCT CCGCTCCCAC 
CGAGTCAACA ATGCCTTCCT GTTCGCCATC AAGAGCGGGA GGAAGAAGCT GCAGCTGGGA 
GTGCAGTTCA TCCCCGGCAA GATCGCCGTC TATGTGGGCC AACGGCGCTC TGTGCACTTT 
GACTACGAGG TCCACGATGG CCAGTGGCAT CACCTGGCCA TTGATATCCG GGGCCGAGCG 
GTCACCTTAT TTACTTCTTG TGGGAAGCGC CGAGTACATG CAGATCTGCA TTTTAAAAAA 
GACACGGCAC TAGACCCCGA GGGGTCGTTC CTCCTCGGGA AAATGAACCA GCACTCGACC 
CCATTCGAGG GCGCCGTGCG CCAGTTTGAT ATCTATCCCT CTGCCCGGGC AGCTCCTAAT 
TACTGGGCCA GCACCCTCCA CCCCACCGTC AGACCGGTGC TCCCTACCTC TGCACCCCAA 
ACTCTCCTGA GCCCTCCTAC CCCCGACTGG GGCCTGCCTG CCCGCCGGGG CCCCACCAGC 
TCCAAGAAGA AGGTGACAGG GCAGAAGGCT GCCAAGAAAG CCCACCTAGA GACGACCCCC 
AAACGAGTCC CTCCCACCAA GCCACGGCAG AAGAAGACCC CAGCAGACGC CCCAGCCAGC 
GACTACCTGC TCTTTGATCC CATCGGGCCG ACCCTCTTCC CGTTCCTGCT GGGACCCCCA 
GGCCAGAAAG GAGACACCGG AGCTGTGGGT TCTCCTGGCC CTCACGGAAA CCCTGGTCGC 
CCCGGCCCCC CTGGTGCTAA AGGCCACAAA GGGGATCCTG GAAGGTCACC TGGAAAGGCC 
TACAATGGGG AAAAGGGAAA TGCAGGCTTA CCTGGATTGA CAGGGCAGCT GGGACCAGTT 
GGTCGGAAGG GGCAGAAGGG CTACCCGGGA CCATCAGGGC ATCCCGGAGA CCAGGGGGAG 
CCAGGACCAG CGGGAAGTCC GGGTGCCAAA GGTTATCCTG GCAGGCAGGG ATTACCTGGA 
GCCATAGGAG ACCCAGGACC CAAAGGCAGC CGGGTAAGTG GTGCTTTGCC GGTGTTTTGG 
GTTCAGCCAG TTGGGCAGCA GGGCCGAGGT GTACCCGGAG TGCCTGGCCA GAGGGGCAAG 
ATGGGTAGGC CGGGCTTCCC AGGAGACTTC GGGGAAAGAG GACCCCCTGG AACGGATGGC 
AACCCCGGTG AACTGGGTGC CCCAGGCCCC CCTGGTGTTC CTGGGCTCAT TGGTGACATG 
GGATTGTTGG GGCCAATTGG GTACCCGGGA CCCAAAGGAT TGAAGGGACT GATGGGAAAC 
TTCGGAGAAC ATGGACTGAA AGGTGATAAG GGTGACCAAG GGCTACCAGG GGTTCCCGGA 
GATATCGGAT TCCAAGGAGA CAAGGGTAGC CAGGGCATGG TGGGGGCACC GGGTGTTCGA 
GGGAAGCCAG GGCCTCCTGG TAAGTCTGGT GACAAAGGAC CTGCTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC GGGAGACATC GGACCCCCAG GTGAAAACGG TCCAGAAGGC 
ACCAAGGGTA AGCCAGGAGC CCGAGGTTTA CCGGGACCAA GAGGACAGTC CGGCCAGGAG 
GGAGATGAAG GACCATCGGG GCCCCCGGGA TCACCTGGCC CAGAGGGCCA CCCTGGACGA 
AAAGGGTTTC CCGGAAGGCC TGGACCCAAC GGCCTAAAGG GGGAGCCAGG AAATCCAGGC 
CGACCTGGAA AAGTAGGGGA ACAGGGTTTG ATGGGATTTA TCGGTCTTGT AGGGGAGCCA 
GGAATCGTGG GAGAGAAGGG GGCCCGAGGG CCAGACGGAC CGACCGGGGA GCCGGGGTCG 
CGGGGTGTGA AGCTCAGTAT CAGGGTCTTC AAGCAAAAAG TTCCCTCCCG ACCCAGCTTC 
TCTCAAAGTC CCCCAGCCCT GGACCAGGCT CCCAGGACCC GAGGCGGCCT GCTCAGGGGA 
CCGCAGGGAA CCCAGGGCAA ACAGGGTCTA ACCGGACAGC AGGGTATCCA GGGTCCATCC 
GGCCCTCCCG GGGCTAAGGG AATTCCTGGG GAACCGGTGA AAGCAGTAGA AAGTGATTTT 
GTTTCAGATA GCTTCCTGTC ATGCTACAGT ATTGATTCCA CCACCAAAAG GCTGAATTTG 
CTAGGACAGT ATCTCAAGGC CAGAGGGCCC TCCCGGTGCA TTAGGAGAGC CTGGCCCTCC 
TGGGGAACCA GGGATGAAGT TCCCAGCCCC CTGGTCCGGT TGGCCCTACT CTTCCTGCTG 
GCCCAGGTCC TCCAGCCTCC CGTCCTGGGC TGTGAGCCGC AGCCTCGGAC CCTGGACAAC 
GTCACTGTCT CCAAGCTGTT CGGCCAGTGG TACTTTGTAG CTGGGGCATC TTCGCTGCCC 
AGGCACCGGC TGGAGATGCG GCTCATCGAT AGCGGGGTGT TGCAGGTGTC CCCCTCGGGG 
AGCCAGGACC GCCTGGACAT CACCCAGCAC CTGAGACTGG GGAACACATG TCTCTCTGAC 
AACAGCAGCT CCATCAAGAT CACTCAGGAC TACACCACGG GCTCTTCCCA TTCTGATCTG 
CTGGTGTTGG ACCAGGGAGG GGAGATCTTT AAGACCCTAC ACTACCTCAG CAACCTCATC 
CAGAGCATCA AGAAGCCACT GGGCACCAAG GAGAACCCTG CTCGCATCTG TCGAGACCTC 
ATGAACTGTG AGCAGAAGAT GGCTGACGGC AAATACTGGA TCGACCCCAA CCTTGGCTGT 
TCTTCAGACA CCATCGAAGT GACCTGTAAC TTCACCAAAG GAGGTCAAAC CTGTCTAAAA 
CCCATCACTG CCTCAAAGGT ACAGAGTTGC ATGGTGCCAG GGGAAAGCCC AAATTGTGCA 
ATGGTGTTAG TGGTGGTGGT GGTAGTTGGA GGAGATGCAG GAGCAAGAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_029062842.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918675.1 Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc127552
Clone ID Related Accession (Same CDS sequence)	XM_029062842.1
Accession Version	XM_029062842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AGTCAGCCCC GGTCTCCAGA TTCAGAGTTG CAGCACCCAG GGGGGGAATC 
TTCTTTCTCT GGATTGTCTT CTGTTCTACC TATTGTCTGG ATTTCACCCA GGGAGTTCCT 
GAAGATGTGG ATGTCCTCCA ACAGCTGGGC CTTGGCAGGA AGCCAAAGGC AGGTGGAAGT 
CCCTTGGCAT CCCACCCTGT CCCCCAGGGG GTCATCCCGT TCCAATCAGG AGTCATCTTC 
ACCCAGCGGG CCCGGGTGGA CGCCCCCACT GGCACGGTCA TCCCTACTGC TTTTGGCACC 
GACCTGGCCC TGGTCCTGAG CCTCCGCTCC CACCGAGTCA ACAATGCCTT CCTGTTCGCC 
ATCAAGAGCG GGAGGAAGAA GCTGCAGCTG GGAGTGCAGT TCATCCCCGG CAAGATCGCC 
GTCTATGTGG GCCAACGGCG CTCTGTGCAC TTTGACTACG AGGTCCACGA TGGCCAGTGG 
CATCACCTGG CCATTGATAT CCGGGGCCGA GCGGTCACCT TATTTACTTC TTGTGGGAAG 
CGCCGAGTAC ATGCAGATCT GCATTTTAAA AAAGACACGG CACTAGACCC CGAGGGGTCG 
TTCCTCCTCG GGAAAATGAA CCAGCACTCG ACCCCATTCG AGGGCGCCGT GTGCCAGTTT 
GATATCTATC CCTCTGCCCA GGCAGCTCAT AATTACTGTA AATACCTGAA ACAACAGTGC 
CGACAGGCAG ACACCTACAG GCCCCATCTG CCCCTACTCG AGCCCCTCTT TCCCAGAGAT 
CCCAGCACGG CAGCGCGGCC GGGACTTCAG ACCGACCTCT GGCTGCCGAG TCTGAGGAAC 
CTGACCACTG TGACGTCGAG GCCGCCGGTG GTCCACGCTG ACCTCGCCCG CCCCCAGCCA 
ACCCCCAGAC CCTCCCGGGG CACGGCGGTC CCCGGGTCGG TGGGACAGCC ACCTGCCCGC 
CGGCCCTCGC GGATGACCAC GACAGCCCCT CGGCCCCGGG CAGGCACCAG CTTGGCCCTA 
ACACGAACCT CTCCCACCAG GCCGGGGAGC ACTAGGGTTG GCTTGGTGAG AGCTGCCTCT 
CCCACTCCCA GGCCAGTGCC GCGGGCAACC AATGCCAAGT CAAGCAAGAC TCCTATTCCA 
GCCAGCAAAC CCCCAGGGCC CAGACCGGCT CGCAGCTCTA CCAAACCCCC TGCTACCCTG 
CTCAGAATGG CACCGCCCCC CTCTGGCGCC ACCTCAGGTC AGGCGGCCCG CTCTGGGCCT 
CGGCCGGCCC CGACCCCCCA CCCCGCCACC AAGTCCATCG CCACCTCTGC CAGGAGCACC 
TCCAAGCCTG TCCCAGACCC CACTGCCCCC CTCCCTGGGA AGCCAGCGTC CCCGACTTCC 
CATGCTGGCC CACCCAGTGC CTCCGTAGTG CCCAGGCCTC TGAGCACCCC AGGAACCAAC 
CTCCCACCCC CCACTGCCAG GACAGCTCCC CTGGTCTCGA GCACTCGTTC CACACGGACA 
TTCCCACCCA TTCTCAGGAC AACGGCAGGA GGCCCCCGGG CTGGTCCCAG GAAAACACCT 
CTTCCCGCCA CCTTGCCAGT CCTGCCTGGC TCAGCCAAAA TGCCCCCTCC AGCTGCCAAG 
CCATCACCCC CTGCCTCCCC TGCCAGCTGG GCCAGCACCC TCCACCCCAC CGTCAGACCG 
GTGCTCCCTA CCTCTGCACC CCAAACTCTC CTGAGCCCTC CTACCCCCGA CTGGGGCCTG 
CCTGCCCGCC GGGGCCCCAC CAGCTCCAAG AAGAAGGTGA CAGGGCAGAA GGCTGCCAAG 
AAAGCCCACC TAGAGACGAC CCCCAAACGA GTCCCTCCCA CCAAGCCACG GCAGAAGAAG 
ACCCCAGCAG ACGCCCCAGG TGACCTCCAT CTCACCCCGC TGCTGCAGAG CTTGGCCAAC 
CTAACGGGTC GGGCTGGGCC CAAGCCATCC CTACCCAGAA GGAGAGCCCC AGTGCCCTCC 
GCGGCCCACC CCACCTCCAG CCCACCACAT CCATTCCCCA GGACCAGTGG CCTGGACCCC 
AGGGGAGCCA CTCCTGTCCC AGCCAGCGAC TACCTGCTCT TTGATCCCAT CGGGCCGACC 
CTCTTCCCGT TCCTGCTGGG ACCCCCAGGC CAGAAAGGAG ACACCGGAGC TGTGGGCCCC 
CCTGGACTTC CTGGGCTGCC TGGACCACCG GGAAAGAGAG GCCCCCGGGG TTCTCCTGGC 
CCTCACGGAA ACCCTGGTCG CCCCGGCCCC CCTGGTGCTA AAGGCCACAA AGGGGATCCT 
GGAAGGTCAC CTGGAAAGGC CTACAATGGG GAAAAGGGAA ATGCAGGCTT ACCTGGATTG 
ACAGGGCAGC TGGGACCAGT TGGTCGGAAG GGGCAGAAGG GCTACCCGGG ACCATCAGGG 
CATCCCGGAG ACCAGGGGGA GCCAGGACCA GCGGGAAGTC CGGGTGCCAA AGGTTATCCT 
GGCAGGCAGG GATTACCTGG AGCCATAGGA GACCCAGGAC CCAAAGGCAG CCGGGGGTAT 
ATTGGTCTCC CCGGCCTGTT CGGCCTGCCA GGATCCGACG GAGAACGAGG TGTACCCGGA 
GTGCCTGGCC AGAGGGGCAA GATGGGTAGG CCGGGCTTCC CAGGAGACTT CGGGGAAAGA 
GGACCCCCTG GAACGGATGG CAACCCCGGT GAACTGGGTG CCCCAGGCCC CCCTGGTGTT 
CCTGGGCTCA TTGGTGACAT GGGATTGTTG GGGCCAATTG GGTACCCGGG ACCCAAAGGA 
TTGAAGGGAC TGATGGGAAA CTTCGGAGAA CATGGACTGA AAGGTGATAA GGGTGACCAA 
GGGCTACCAG GGGTTCCCGG AGATATCGGA TTCCAAGGAG ACAAGGGTAG CCAGGGCATG 
GTGGGGGCAC CGGGTGTTCG AGGGAAGCCA GGGCCTCCTG GTAAGTCTGG TGACAAAGGA 
CCTGCTGGGT TTCCTGGACC TCCTGGACCT GAGGGTTTCC CGGGAGACAT TGGACCCCCA 
GGTGAAAACG GTCCAGAAGG CACCAAGGGT AAGCCAGGAG CCCGAGGTTT ACCGGGACCA 
AGAGGACAGT CCGGCCAGGA GGGAGATGAA GGACCATCGG GGCCCCCGGG ATCACCTGGC 
CCAGAGGGCC ACCCTGGACG AAAAGGGTTT CCCGGAAGGC CTGGACCCAA CGGCCTAAAG 
GGGGAGCCAG GAAATCCAGG CCGACCTGGA AAAGTAGGGG AACAGGGTTT GATGGGATTT 
ATCGGTCTTG TAGGGGAGCC AGGAATCGTG GGAGAGAAGG GTAACCGAGG GCCCATGGGA 
CCACCCGGTG TGCCTGGAGT TAAGGGGTCG ATGGGACACC CTGGGACAAC AGGCAGAACT 
GGAGACCCCG GAGATCCTGG AAATTCAGGT CCTCCTGGAG TTCGTGGGTC TCCTGGGATG 
AGAGGGGTGA AAGGTCGCCG GGGGGCCCGA GGGCCAGACG GACCGACCGG GGAGCCGGGG 
TCGCGGGGTG TGAAGGGACC GCAGGGAACC CAGGGCAAAC AGGGTCTAAC CGGACAGCAG 
GGCAAGGCAG GAGAGCGAGG AGAGACGGGA CCTCGTGGCT TCCCTGGTAT CCAGGGTCCA 
TCCGGCCCTC CCGGGGCTAA GGGAATTCCT GGGGAACCGG GCCCCCCAGG ACCCCAGGGA 
TTGTCTGGCC CTTTGGGAGA GATGGGGCAT AAGGGCCCTC CCGGTGCATT AGGAGAGCCT 
GGCCCTCCTG GGGAACCAGG GATGAAGGGT GATTTTGGAC CATTGGGCAT TCCCGGTGAA 
CAAGGTCTGA TTGGCCAGAG GGGAGAAACT GGGCTGGAAG GAGACAGTGG CCAACCTGGA 
CCTCCTGGAT TGAAGGGAGA CAAGGGAGAG CCAGGCCCTG AAGGCGAGAA AGGAGAGAAA 
GGCAAAGAGG GGCTGAAGGG CATGGAGGGC CCCCCTGGAC TTCCCGGAAT CACAGGTGTC 
CGTGGTCCCG AAGGCAAACC AGGCAAGCAA GGTGAAAAGG GCCGGACAGG ACCAAAGGGC 
GCCAAGGGGT ATCAAGGGCA CCTAGGTGAA ACTGGCGCTC CTGGAGGACC CGGGCCAACT 
GGCACACAAG GCCCTAAAGG CTCCCGAGTT GGTGTCCATC TCCCTGTTAA GAAAGCAACT 
GGTGGTGGGA CCGGGGGAGC TCCAGGGCGG ATGGGAGCCC AAGGAGAACC AGGATTGCCC 
GGTTATGATG GACACAAAGG ATTTCTGGGA CCCATCGGCC CCCCAGGACC CAAAGGCGAG 
AAGGGTGAAC AAGGAGATGA CGGGAAGACA GAGGGTCCCC CTGGACCTCC TGGAGACAGG 
GGTCCGGTTG GTGATCGAGG AGACCGTGGA GAGCACGGGG AGCCGGGTTA CCCAGGCCAG 
GAAGGCCTTG TAGGAAGCCG AGGCAAAGCA GGCCAGCAAG GTCTTCCCGG GCACCCTGGA 
CCACCAGGCC AACCAGGACC CAAGGGATCC AAAGGCGAAG AGGGCCCGAA AGGGAAGCAA 
GGCAAGCGTG GGGAAGCCGG CAGTCGGGGG ATGCAGGGCC CCGTGGGGCA TCCCGGGCCA 
CGGGGCGTGG TGGGGAGACA AGGCCAAGAA GGGCTCCCCG GACCAGATGG GATTCCTGGC 
AGGGACGGCC GAGCGGGACC GCAGGGGGAA CAGGGAGAAG ACGGGGAAGT TGGTGTCATG 
GGTCCTCCTG GGAAGAGAGG GAATCCCGGC GTGGGCGGCC TGCCCGGAGC TCAGGGACCC 
CCAGGATTCA AGGGTGAAAA CGGGTTACCC GGGCCACCGG GTCCCACAGG AAAGCGAGGG 
ACAGAGGGAG GAACGGGGCT TCCCGGAAGC CAGGGGGAGC CAGGATCCAA GGGCCGGCCG 
GGGGACTTTG GCGAGATGGG CTTCCCGGGG ATCGCAGGCC TGTTTGGCCC CAAGGGGCCT 
CCAGGAGATC TTGGTTTCAA AGGAATCCAG GGCCCCAGGG GTCCCCCAGG CCTGATGGGA 
CAAGAAGGTG TCATCGGCCC GCTGGGGATC CTGGGGCCTA CTGGACTCCC GGGCCCAAAA 
GGGGATGAGG GGAGTCGAGG TGAAACGGGA CTTCAAGGCC CAAGGGGTCC CCCAGGTCCC 
CGAGGACGCC CTGGACCGCC GGGCCCTCCA GGGATTCCTG TTCCATTTCA ACAAGATGAC 
CTCGGGACAG CTTTCCAGAC GTGGCTAGAC TCCTCAAACG GTGGCGTGCT CAGGACAGAG 
GGCTCTTCCC ATTCTGATCT GCTGGTGTTG GACCAGGGAG GGGAGATCTT TAAGACCCTA 
CACTACCTCA GCAACCTCAT CCAGAGCATC AAGAAGCCAC TGGGCACCAA GGAGAACCCT 
GCTCGCATCT GTCGAGACCT CATGAACTGT GAGCAGAAGA TGGCTGACGG CAAATACTGG 
ATCGACCCCA ACCTTGGCTG TTCTTCAGAC ACCATCGAAG TGACCTGTAA CTTCACCAAA 
GGAGGTCAAA CCTGTCTAAA ACCCATCACT GCCTCAAAGG TGGAGTTCGC CATCGGCCGG 
GTCCAGATGA ACTTCCTACA CCTCCTGAGC TCCGAAGTGG TCCAGCACCT GACCATCCAC 
TGCCTCGATG TGTCCGTGTG GCAGGAAGGG CAAGGCCGGC CACCCAGCAA GAAGTCTCTG 
CGCTTCCGGG CCTGGAACGG ACAAATCTAT GAGGCTGGAG GCCAGTTCCG ACCAGAGGTC 
TCTGTGGATG ACTGCAAGGT TCAGGACGGA CGCTGGCACC AAACCCGCTT CACGTTCCGG 
ACACAGGACC CCCAGCAGCT GCCAATCGTC AGCATCTACA ATCTGCCGCC CACCACCCCG 
GGGAAGCAGT ACCGCCTGGA AGTCGGGTCT GTGTGCTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918675.1
CDS	401..6265
Translation	MEPESAPVSRFRVAAPRGGIFFLWIVFCSTYCLDFTQGVPEDVDVLQQLGLGRKPKAGGSPLASHPVPQGVIPFQSGVIFTQRARVDAPTGTVIPTAFGTDLALVLSLRSHRVNNAFLFAIKSGRKKLQLGVQFIPGKIAVYVGQRRSVHFDYEVHDGQWHHLAIDIRGRAVTLFTSCGKRRVHADLHFKKDTALDPEGSFLLGKMNQHSTPFEGAVCQFDIYPSAQAAHNYCKYLKQQCRQADTYRPHLPLLEPLFPRDPSTAARPGLQTDLWLPSLRNLTTVTSRPPVVHADLARPQPTPRPSRGTAVPGSVGQPPARRPSRMTTTAPRPRAGTSLALTRTSPTRPGSTRVGLVRAASPTPRPVPRATNAKSSKTPIPASKPPGPRPARSSTKPPATLLRMAPPPSGATSGQAARSGPRPAPTPHPATKSIATSARSTSKPVPDPTAPLPGKPASPTSHAGPPSASVVPRPLSTPGTNLPPPTARTAPLVSSTRSTRTFPPILRTTAGGPRAGPRKTPLPATLPVLPGSAKMPPPAAKPSPPASPASWASTLHPTVRPVLPTSAPQTLLSPPTPDWGLPARRGPTSSKKKVTGQKAAKKAHLETTPKRVPPTKPRQKKTPADAPGDLHLTPLLQSLANLTGRAGPKPSLPRRRAPVPSAAHPTSSPPHPFPRTSGLDPRGATPVPASDYLLFDPIGPTLFPFLLGPPGQKGDTGAVGPPGLPGLPGPPGKRGPRGSPGPHGNPGRPGPPGAKGHKGDPGRSPGKAYNGEKGNAGLPGLTGQLGPVGRKGQKGYPGPSGHPGDQGEPGPAGSPGAKGYPGRQGLPGAIGDPGPKGSRGYIGLPGLFGLPGSDGERGVPGVPGQRGKMGRPGFPGDFGERGPPGTDGNPGELGAPGPPGVPGLIGDMGLLGPIGYPGPKGLKGLMGNFGEHGLKGDKGDQGLPGVPGDIGFQGDKGSQGMVGAPGVRGKPGPPGKSGDKGPAGFPGPPGPEGFPGDIGPPGENGPEGTKGKPGARGLPGPRGQSGQEGDEGPSGPPGSPGPEGHPGRKGFPGRPGPNGLKGEPGNPGRPGKVGEQGLMGFIGLVGEPGIVGEKGNRGPMGPPGVPGVKGSMGHPGTTGRTGDPGDPGNSGPPGVRGSPGMRGVKGRRGARGPDGPTGEPGSRGVKGPQGTQGKQGLTGQQGKAGERGETGPRGFPGIQGPSGPPGAKGIPGEPGPPGPQGLSGPLGEMGHKGPPGALGEPGPPGEPGMKGDFGPLGIPGEQGLIGQRGETGLEGDSGQPGPPGLKGDKGEPGPEGEKGEKGKEGLKGMEGPPGLPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGETGAPGGPGPTGTQGPKGSRVGVHLPVKKATGGGTGGAPGRMGAQGEPGLPGYDGHKGFLGPIGPPGPKGEKGEQGDDGKTEGPPGPPGDRGPVGDRGDRGEHGEPGYPGQEGLVGSRGKAGQQGLPGHPGPPGQPGPKGSKGEEGPKGKQGKRGEAGSRGMQGPVGHPGPRGVVGRQGQEGLPGPDGIPGRDGRAGPQGEQGEDGEVGVMGPPGKRGNPGVGGLPGAQGPPGFKGENGLPGPPGPTGKRGTEGGTGLPGSQGEPGSKGRPGDFGEMGFPGIAGLFGPKGPPGDLGFKGIQGPRGPPGLMGQEGVIGPLGILGPTGLPGPKGDEGSRGETGLQGPRGPPGPRGRPGPPGPPGIPVPFQQDDLGTAFQTWLDSSNGGVLRTEGSSHSDLLVLDQGGEIFKTLHYLSNLIQSIKKPLGTKENPARICRDLMNCEQKMADGKYWIDPNLGCSSDTIEVTCNFTKGGQTCLKPITASKVEFAIGRVQMNFLHLLSSEVVQHLTIHCLDVSVWQEGQGRPPSKKSLRFRAWNGQIYEAGGQFRPEVSVDDCKVQDGRWHQTRFTFRTQDPQQLPIVSIYNLPPTTPGKQYRLEVGSVCFL

Target ORF information:

RefSeq Version	XM_029062842.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029062842.1

ORF Insert Sequence:

ATGGAGCCGG AGTCAGCCCC GGTCTCCAGA TTCAGAGTTG CAGCACCCAG GGGGGGAATC 
TTCTTTCTCT GGATTGTCTT CTGTTCTACC TATTGTCTGG ATTTCACCCA GGGAGTTCCT 
GAAGATGTGG ATGTCCTCCA ACAGCTGGGC CTTGGCAGGA AGCCAAAGGC AGGTGGAAGT 
CCCTTGGCAT CCCACCCTGT CCCCCAGGGG GTCATCCCGT TCCAATCAGG AGTCATCTTC 
ACCCAGCGGG CCCGGGTGGA CGCCCCCACT GGCACGGTCA TCCCTACTGC TTTTGGCACC 
GACCTGGCCC TGGTCCTGAG CCTCCGCTCC CACCGAGTCA ACAATGCCTT CCTGTTCGCC 
ATCAAGAGCG GGAGGAAGAA GCTGCAGCTG GGAGTGCAGT TCATCCCCGG CAAGATCGCC 
GTCTATGTGG GCCAACGGCG CTCTGTGCAC TTTGACTACG AGGTCCACGA TGGCCAGTGG 
CATCACCTGG CCATTGATAT CCGGGGCCGA GCGGTCACCT TATTTACTTC TTGTGGGAAG 
CGCCGAGTAC ATGCAGATCT GCATTTTAAA AAAGACACGG CACTAGACCC CGAGGGGTCG 
TTCCTCCTCG GGAAAATGAA CCAGCACTCG ACCCCATTCG AGGGCGCCGT GTGCCAGTTT 
GATATCTATC CCTCTGCCCA GGCAGCTCAT AATTACTGTA AATACCTGAA ACAACAGTGC 
CGACAGGCAG ACACCTACAG GCCCCATCTG CCCCTACTCG AGCCCCTCTT TCCCAGAGAT 
CCCAGCACGG CAGCGCGGCC GGGACTTCAG ACCGACCTCT GGCTGCCGAG TCTGAGGAAC 
CTGACCACTG TGACGTCGAG GCCGCCGGTG GTCCACGCTG ACCTCGCCCG CCCCCAGCCA 
ACCCCCAGAC CCTCCCGGGG CACGGCGGTC CCCGGGTCGG TGGGACAGCC ACCTGCCCGC 
CGGCCCTCGC GGATGACCAC GACAGCCCCT CGGCCCCGGG CAGGCACCAG CTTGGCCCTA 
ACACGAACCT CTCCCACCAG GCCGGGGAGC ACTAGGGTTG GCTTGGTGAG AGCTGCCTCT 
CCCACTCCCA GGCCAGTGCC GCGGGCAACC AATGCCAAGT CAAGCAAGAC TCCTATTCCA 
GCCAGCAAAC CCCCAGGGCC CAGACCGGCT CGCAGCTCTA CCAAACCCCC TGCTACCCTG 
CTCAGAATGG CACCGCCCCC CTCTGGCGCC ACCTCAGGTC AGGCGGCCCG CTCTGGGCCT 
CGGCCGGCCC CGACCCCCCA CCCCGCCACC AAGTCCATCG CCACCTCTGC CAGGAGCACC 
TCCAAGCCTG TCCCAGACCC CACTGCCCCC CTCCCTGGGA AGCCAGCGTC CCCGACTTCC 
CATGCTGGCC CACCCAGTGC CTCCGTAGTG CCCAGGCCTC TGAGCACCCC AGGAACCAAC 
CTCCCACCCC CCACTGCCAG GACAGCTCCC CTGGTCTCGA GCACTCGTTC CACACGGACA 
TTCCCACCCA TTCTCAGGAC AACGGCAGGA GGCCCCCGGG CTGGTCCCAG GAAAACACCT 
CTTCCCGCCA CCTTGCCAGT CCTGCCTGGC TCAGCCAAAA TGCCCCCTCC AGCTGCCAAG 
CCATCACCCC CTGCCTCCCC TGCCAGCTGG GCCAGCACCC TCCACCCCAC CGTCAGACCG 
GTGCTCCCTA CCTCTGCACC CCAAACTCTC CTGAGCCCTC CTACCCCCGA CTGGGGCCTG 
CCTGCCCGCC GGGGCCCCAC CAGCTCCAAG AAGAAGGTGA CAGGGCAGAA GGCTGCCAAG 
AAAGCCCACC TAGAGACGAC CCCCAAACGA GTCCCTCCCA CCAAGCCACG GCAGAAGAAG 
ACCCCAGCAG ACGCCCCAGG TGACCTCCAT CTCACCCCGC TGCTGCAGAG CTTGGCCAAC 
CTAACGGGTC GGGCTGGGCC CAAGCCATCC CTACCCAGAA GGAGAGCCCC AGTGCCCTCC 
GCGGCCCACC CCACCTCCAG CCCACCACAT CCATTCCCCA GGACCAGTGG CCTGGACCCC 
AGGGGAGCCA CTCCTGTCCC AGCCAGCGAC TACCTGCTCT TTGATCCCAT CGGGCCGACC 
CTCTTCCCGT TCCTGCTGGG ACCCCCAGGC CAGAAAGGAG ACACCGGAGC TGTGGGCCCC 
CCTGGACTTC CTGGGCTGCC TGGACCACCG GGAAAGAGAG GCCCCCGGGG TTCTCCTGGC 
CCTCACGGAA ACCCTGGTCG CCCCGGCCCC CCTGGTGCTA AAGGCCACAA AGGGGATCCT 
GGAAGGTCAC CTGGAAAGGC CTACAATGGG GAAAAGGGAA ATGCAGGCTT ACCTGGATTG 
ACAGGGCAGC TGGGACCAGT TGGTCGGAAG GGGCAGAAGG GCTACCCGGG ACCATCAGGG 
CATCCCGGAG ACCAGGGGGA GCCAGGACCA GCGGGAAGTC CGGGTGCCAA AGGTTATCCT 
GGCAGGCAGG GATTACCTGG AGCCATAGGA GACCCAGGAC CCAAAGGCAG CCGGGGGTAT 
ATTGGTCTCC CCGGCCTGTT CGGCCTGCCA GGATCCGACG GAGAACGAGG TGTACCCGGA 
GTGCCTGGCC AGAGGGGCAA GATGGGTAGG CCGGGCTTCC CAGGAGACTT CGGGGAAAGA 
GGACCCCCTG GAACGGATGG CAACCCCGGT GAACTGGGTG CCCCAGGCCC CCCTGGTGTT 
CCTGGGCTCA TTGGTGACAT GGGATTGTTG GGGCCAATTG GGTACCCGGG ACCCAAAGGA 
TTGAAGGGAC TGATGGGAAA CTTCGGAGAA CATGGACTGA AAGGTGATAA GGGTGACCAA 
GGGCTACCAG GGGTTCCCGG AGATATCGGA TTCCAAGGAG ACAAGGGTAG CCAGGGCATG 
GTGGGGGCAC CGGGTGTTCG AGGGAAGCCA GGGCCTCCTG GTAAGTCTGG TGACAAAGGA 
CCTGCTGGGT TTCCTGGACC TCCTGGACCT GAGGGTTTCC CGGGAGACAT TGGACCCCCA 
GGTGAAAACG GTCCAGAAGG CACCAAGGGT AAGCCAGGAG CCCGAGGTTT ACCGGGACCA 
AGAGGACAGT CCGGCCAGGA GGGAGATGAA GGACCATCGG GGCCCCCGGG ATCACCTGGC 
CCAGAGGGCC ACCCTGGACG AAAAGGGTTT CCCGGAAGGC CTGGACCCAA CGGCCTAAAG 
GGGGAGCCAG GAAATCCAGG CCGACCTGGA AAAGTAGGGG AACAGGGTTT GATGGGATTT 
ATCGGTCTTG TAGGGGAGCC AGGAATCGTG GGAGAGAAGG GTAACCGAGG GCCCATGGGA 
CCACCCGGTG TGCCTGGAGT TAAGGGGTCG ATGGGACACC CTGGGACAAC AGGCAGAACT 
GGAGACCCCG GAGATCCTGG AAATTCAGGT CCTCCTGGAG TTCGTGGGTC TCCTGGGATG 
AGAGGGGTGA AAGGTCGCCG GGGGGCCCGA GGGCCAGACG GACCGACCGG GGAGCCGGGG 
TCGCGGGGTG TGAAGGGACC GCAGGGAACC CAGGGCAAAC AGGGTCTAAC CGGACAGCAG 
GGCAAGGCAG GAGAGCGAGG AGAGACGGGA CCTCGTGGCT TCCCTGGTAT CCAGGGTCCA 
TCCGGCCCTC CCGGGGCTAA GGGAATTCCT GGGGAACCGG GCCCCCCAGG ACCCCAGGGA 
TTGTCTGGCC CTTTGGGAGA GATGGGGCAT AAGGGCCCTC CCGGTGCATT AGGAGAGCCT 
GGCCCTCCTG GGGAACCAGG GATGAAGGGT GATTTTGGAC CATTGGGCAT TCCCGGTGAA 
CAAGGTCTGA TTGGCCAGAG GGGAGAAACT GGGCTGGAAG GAGACAGTGG CCAACCTGGA 
CCTCCTGGAT TGAAGGGAGA CAAGGGAGAG CCAGGCCCTG AAGGCGAGAA AGGAGAGAAA 
GGCAAAGAGG GGCTGAAGGG CATGGAGGGC CCCCCTGGAC TTCCCGGAAT CACAGGTGTC 
CGTGGTCCCG AAGGCAAACC AGGCAAGCAA GGTGAAAAGG GCCGGACAGG ACCAAAGGGC 
GCCAAGGGGT ATCAAGGGCA CCTAGGTGAA ACTGGCGCTC CTGGAGGACC CGGGCCAACT 
GGCACACAAG GCCCTAAAGG CTCCCGAGTT GGTGTCCATC TCCCTGTTAA GAAAGCAACT 
GGTGGTGGGA CCGGGGGAGC TCCAGGGCGG ATGGGAGCCC AAGGAGAACC AGGATTGCCC 
GGTTATGATG GACACAAAGG ATTTCTGGGA CCCATCGGCC CCCCAGGACC CAAAGGCGAG 
AAGGGTGAAC AAGGAGATGA CGGGAAGACA GAGGGTCCCC CTGGACCTCC TGGAGACAGG 
GGTCCGGTTG GTGATCGAGG AGACCGTGGA GAGCACGGGG AGCCGGGTTA CCCAGGCCAG 
GAAGGCCTTG TAGGAAGCCG AGGCAAAGCA GGCCAGCAAG GTCTTCCCGG GCACCCTGGA 
CCACCAGGCC AACCAGGACC CAAGGGATCC AAAGGCGAAG AGGGCCCGAA AGGGAAGCAA 
GGCAAGCGTG GGGAAGCCGG CAGTCGGGGG ATGCAGGGCC CCGTGGGGCA TCCCGGGCCA 
CGGGGCGTGG TGGGGAGACA AGGCCAAGAA GGGCTCCCCG GACCAGATGG GATTCCTGGC 
AGGGACGGCC GAGCGGGACC GCAGGGGGAA CAGGGAGAAG ACGGGGAAGT TGGTGTCATG 
GGTCCTCCTG GGAAGAGAGG GAATCCCGGC GTGGGCGGCC TGCCCGGAGC TCAGGGACCC 
CCAGGATTCA AGGGTGAAAA CGGGTTACCC GGGCCACCGG GTCCCACAGG AAAGCGAGGG 
ACAGAGGGAG GAACGGGGCT TCCCGGAAGC CAGGGGGAGC CAGGATCCAA GGGCCGGCCG 
GGGGACTTTG GCGAGATGGG CTTCCCGGGG ATCGCAGGCC TGTTTGGCCC CAAGGGGCCT 
CCAGGAGATC TTGGTTTCAA AGGAATCCAG GGCCCCAGGG GTCCCCCAGG CCTGATGGGA 
CAAGAAGGTG TCATCGGCCC GCTGGGGATC CTGGGGCCTA CTGGACTCCC GGGCCCAAAA 
GGGGATGAGG GGAGTCGAGG TGAAACGGGA CTTCAAGGCC CAAGGGGTCC CCCAGGTCCC 
CGAGGACGCC CTGGACCGCC GGGCCCTCCA GGGATTCCTG TTCCATTTCA ACAAGATGAC 
CTCGGGACAG CTTTCCAGAC GTGGCTAGAC TCCTCAAACG GTGGCGTGCT CAGGACAGAG 
GGCTCTTCCC ATTCTGATCT GCTGGTGTTG GACCAGGGAG GGGAGATCTT TAAGACCCTA 
CACTACCTCA GCAACCTCAT CCAGAGCATC AAGAAGCCAC TGGGCACCAA GGAGAACCCT 
GCTCGCATCT GTCGAGACCT CATGAACTGT GAGCAGAAGA TGGCTGACGG CAAATACTGG 
ATCGACCCCA ACCTTGGCTG TTCTTCAGAC ACCATCGAAG TGACCTGTAA CTTCACCAAA 
GGAGGTCAAA CCTGTCTAAA ACCCATCACT GCCTCAAAGG TGGAGTTCGC CATCGGCCGG 
GTCCAGATGA ACTTCCTACA CCTCCTGAGC TCCGAAGTGG TCCAGCACCT GACCATCCAC 
TGCCTCGATG TGTCCGTGTG GCAGGAAGGG CAAGGCCGGC CACCCAGCAA GAAGTCTCTG 
CGCTTCCGGG CCTGGAACGG ACAAATCTAT GAGGCTGGAG GCCAGTTCCG ACCAGAGGTC 
TCTGTGGATG ACTGCAAGGT TCAGGACGGA CGCTGGCACC AAACCCGCTT CACGTTCCGG 
ACACAGGACC CCCAGCAGCT GCCAATCGTC AGCATCTACA ATCTGCCGCC CACCACCCCG 
GGGAAGCAGT ACCGCCTGGA AGTCGGGTCT GTGTGCTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_029062843.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918676.1 Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

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CloneID	OOc127553
Clone ID Related Accession (Same CDS sequence)	XM_029062843.1
Accession Version	XM_029062843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AGTCAGCCCC GGTCTCCAGA TTCAGAGTTG CAGCACCCAG GGGGGGAATC 
TTCTTTCTCT GGATTGTCTT CTGTTCTACC TATTGTCTGG ATTTCACCCA GGGAGTTCCT 
GAAGATGTGG ATGTCCTCCA ACAGCTGGGC CTTGGCAGGA AGCCAAAGGC AGGTGGAAGT 
CCCTTGGCAT CCCACCCTGT CCCCCAGGGG GTCATCCCGT TCCAATCAGG AGTCATCTTC 
ACCCAGCGGG CCCGGGTGGA CGCCCCCACT GGCACGGTCA TCCCTACTGC TTTTGGCACC 
GACCTGGCCC TGGTCCTGAG CCTCCGCTCC CACCGAGTCA ACAATGCCTT CCTGTTCGCC 
ATCAAGAGCG GGAGGAAGAA GCTGCAGCTG GGAGTGCAGT TCATCCCCGG CAAGATCGCC 
GTCTATGTGG GCCAACGGCG CTCTGTGCAC TTTGACTACG AGGTCCACGA TGGCCAGTGG 
CATCACCTGG CCATTGATAT CCGGGGCCGA GCGGTCACCT TATTTACTTC TTGTGGGAAG 
CGCCGAGTAC ATGCAGATCT GCATTTTAAA AAAGACACGG CACTAGACCC CGAGGGGTCG 
TTCCTCCTCG GGAAAATGAA CCAGCACTCG ACCCCATTCG AGGGCGCCGT GTGCCAGTTT 
GATATCTATC CCTCTGCCCA GGCAGCTCAT AATTACTGTA AATACCTGAA ACAACAGTGC 
CGACAGGCAG ACACCTACAG GCCCCATCTG CCCCTACTCG AGCCCCTCTT TCCCAGAGAT 
CCCAGCACGG CAGCGCGGCC GGGACTTCAG ACCGACCTCT GGCTGCCGAG TCTGAGGAAC 
CTGACCACTG TGACGTCGAG GCCGCCGGTG GTCCACGCTG ACCTCGCCCG CCCCCAGCCA 
ACCCCCAGAC CCTCCCGGGG CACGGCGGTC CCCGGGTCGG TGGGACAGCC ACCTGCCCGC 
CGGCCCTCGC GGATGACCAC GACAGCCCCT CGGCCCCGGG CAGGCACCAG CTTGGCCCTA 
ACACGAACCT CTCCCACCAG GCCGGGGAGC ACTAGGGTTG GCTTGGTGAG AGCTGCCTCT 
CCCACTCCCA GGCCAGTGCC GCGGGCAACC AATGCCAAGT CAAGCAAGAC TCCTATTCCA 
GCCAGCAAAC CCCCAGGGCC CAGACCGGCT CGCAGCTCTA CCAAACCCCC TGCTACCCTG 
CTCAGAATGG CACCGCCCCC CTCTGGCGCC ACCTCAGGTC AGGCGGCCCG CTCTGGGCCT 
CGGCCGGCCC CGACCCCCCA CCCCGCCACC AAGTCCATCG CCACCTCTGC CAGGAGCACC 
TCCAAGCCTG TCCCAGACCC CACTGCCCCC CTCCCTGGGA AGCCAGCGTC CCCGACTTCC 
CATGCTGGCC CACCCAGTGC CTCCGTAGTG CCCAGGCCTC TGAGCACCCC AGGAACCAAC 
CTCCCACCCC CCACTGCCAG GACAGCTCCC CTGGTCTCGA GCACTCGTTC CACACGGACA 
TTCCCACCCA TTCTCAGGAC AACGGCAGGA GGCCCCCGGG CTGGTCCCAG GAAAACACCT 
CTTCCCGCCA CCTTGCCAGT CCTGCCTGGC TCAGCCAAAA TGCCCCCTCC AGCTGCCAAG 
CCATCACCCC CTGCCTCCCC TGCCAGCTGG GCCAGCACCC TCCACCCCAC CGTCAGACCG 
GTGCTCCCTA CCTCTGCACC CCAAACTCTC CTGAGCCCTC CTACCCCCGA CTGGGGCCTG 
CCTGCCCGCC GGGGCCCCAC CAGCTCCAAG AAGAAGGTGA CAGGGCAGAA GGCTGCCAAG 
AAAGCCCACC TAGAGACGAC CCCCAAACGA GTCCCTCCCA CCAAGCCACG GCAGAAGAAG 
ACCCCAGCAG ACGCCCCAGG TGACCTCCAT CTCACCCCGC TGCTGCAGAG CTTGGCCAAC 
CTAACGGGTC GGGCTGGGCC CAAGCCATCC CTACCCAGAA GGAGAGCCCC AGTGCCCTCC 
GCGGCCCACC CCACCTCCAG CCCACCACAT CCATTCCCCA GGACCAGTGG CCTGGACCCC 
AGGGGAGCCA CTCCTGTCCC AGCCAGCGAC TACCTGCTCT TTGATCCCAT CGGGCCGACC 
CTCTTCCCGT TCCTGCTGGG ACCCCCAGGC CAGAAAGGAG ACACCGGAGC TGTGGGCCCC 
CCTGGACTTC CTGGGCTGCC TGGACCACCG GGAAAGAGAG GCCCCCGGGG TTCTCCTGGC 
CCTCACGGAA ACCCTGGTCG CCCCGGCCCC CCTGGTGCTA AAGGCCACAA AGGGGATCCT 
GGAAGGTCAC CTGGAAAGGC CTACAATGGG GAAAAGGGAA ATGCAGGCTT ACCTGGATTG 
ACAGGGCAGC TGGGACCAGT TGGTCGGAAG GGGCAGAAGG GCTACCCGGG ACCATCAGGG 
CATCCCGGAG ACCAGGGGGA GCCAGGACCA GCGGGAAGTC CGGGTGCCAA AGGTTATCCT 
GGCAGGCAGG GATTACCTGG AGCCATAGGA GACCCAGGAC CCAAAGGCAG CCGGGGGTAT 
ATTGGTCTCC CCGGCCTGTT CGGCCTGCCA GGATCCGACG GAGAACGAGG TGTACCCGGA 
GTGCCTGGCC AGAGGGGCAA GATGGGTAGG CCGGGCTTCC CAGGAGACTT CGGGGAAAGA 
GGACCCCCTG GAACGGATGG CAACCCCGGT GAACTGGGTG CCCCAGGCCC CCCTGGTGTT 
CCTGGGCTCA TTGGTGACAT GGGATTGTTG GGGCCAATTG GGTACCCGGG ACCCAAAGGA 
TTGAAGGGAC TGATGGGAAA CTTCGGAGAA CATGGACTGA AAGGTGATAA GGGTGACCAA 
GGGCTACCAG GGGTTCCCGG AGATATCGGA TTCCAAGGAG ACAAGGGTAG CCAGGGCATG 
GTGGGGGCAC CGGGTGTTCG AGGGAAGCCA GGGCCTCCTG GTAAGTCTGG TGACAAAGGA 
CCTGCTGGGT TTCCTGGACC TCCTGGACCT GAGGGTTTCC CGGGAGACAT TGGACCCCCA 
GGTGAAAACG GTCCAGAAGG CACCAAGGGT AAGCCAGGAG CCCGAGGTTT ACCGGGACCA 
AGAGGACAGT CCGGCCAGGA GGGAGATGAA GGACCATCGG GGCCCCCGGG ATCACCTGGC 
CCAGAGGGCC ACCCTGGACG AAAAGGGTTT CCCGGAAGGC CTGGACCCAA CGGCCTAAAG 
GGGGAGCCAG GAAATCCAGG CCGACCTGGA AAAGTAGGGG AACAGGGTTT GATGGGATTT 
ATCGGTCTTG TAGGGGAGCC AGGAATCGTG GGAGAGAAGG GTAACCGAGG GCCCATGGGA 
CCACCCGGTG TGCCTGGAGT TAAGGGGTCG ATGGGACACC CTGGGACAAC AGGCAGAACT 
GGAGACCCCG GAGATCCTGG AAATTCAGGT CCTCCTGGAG TTCGTGGGTC TCCTGGGATG 
AGAGGGGTGA AAGGTCGCCG GGGGGCCCGA GGGCCAGACG GACCGACCGG GGAGCCGGGG 
TCGCGGGGTG TGAAGGGACC GCAGGGAACC CAGGGCAAAC AGGGTCTAAC CGGACAGCAG 
GGCAAGGCAG GAGAGCGAGG AGAGACGGGA CCTCGTGGCT TCCCTGGTAT CCAGGGTCCA 
TCCGGCCCTC CCGGGGCTAA GGGAATTCCT GGGGAACCGG GCCCCCCAGG ACCCCAGGGA 
TTGTCTGGCC CTTTGGGAGA GATGGGGCAT AAGGGCCCTC CCGGTGCATT AGGAGAGCCT 
GGCCCTCCTG GGGAACCAGG GATGAAGGGT GATTTTGGAC CATTGGGCAT TCCCGGTGAA 
CAAGGTCTGA TTGGCCAGAG GGGAGAAACT GGGCTGGAAG GAGACAGTGG CCAACCTGGA 
CCTCCTGGAT TGAAGGGAGA CAAGGGAGAG CCAGGCCCTG AAGGCGAGAA AGGAGAGAAA 
GGCAAAGAGG GGCTGAAGGG CATGGAGGGC CCCCCTGGAC TTCCCGGAAT CACAGGTGTC 
CGTGGTCCCG AAGGCAAACC AGGCAAGCAA GGTGAAAAGG GCCGGACAGG ACCAAAGGGC 
GCCAAGGGGT ATCAAGGGCA CCTAGGTGAA ACTGGCGCTC CTGGAGGACC CGGGCCAACT 
GGCACACAAG GCCCTAAAGG CTCCCGAGGT ACCCTGGGAC CGACGGGAGC TCCAGGGCGG 
ATGGGAGCCC AAGGAGAACC AGGATTGCCC GGTTATGATG GACACAAAGG ATTTCTGGGA 
CCCATCGGCC CCCCAGGACC CAAAGGCGAG AAGGGTGAAC AAGGAGATGA CGGGAAGACA 
GAGGGTCCCC CTGGACCTCC TGGAGACAGG GGTCCGGTTG GTGATCGAGG AGACCGTGGA 
GAGCACGGGG AGCCGGGTTA CCCAGGCCAG GAAGGCCTTG TAGGAAGCCG AGGCAAAGCA 
GGCCAGCAAG GTCTTCCCGG GCACCCTGGA CCACCAGGCC AACCAGGACC CAAGGGATCC 
AAAGGCGAAG AGGGCCCGAA AGGGAAGCAA GGCAAGCGTG GGGAAGCCGG CAGTCGGGGG 
ATGCAGGGCC CCGTGGGGCA TCCCGGGCCA CGGGGCGTGG TGGGGAGACA AGGCCAAGAA 
GGGCTCCCCG GACCAGATGG GATTCCTGGC AGGGACGGCC GAGCGGGACC GCAGGGGGAA 
CAGGGAGAAG ACGGGGAAGT TGGTGTCATG GGTCCTCCTG GGAAGAGAGG GAATCCCGGC 
GTGGGCGGCC TGCCCGGAGC TCAGGGACCC CCAGGATTCA AGGGTGAAAA CGGGTTACCC 
GGGCCACCGG GTCCCACAGG AAAGCGAGGG ACAGAGGGAG GAACGGGGCT TCCCGGAAGC 
CAGGGGGAGC CAGGATCCAA GGGCCGGCCG GGGGACTTTG GCGAGATGGG CTTCCCGGGG 
ATCGCAGGCC TGTTTGGCCC CAAGGGGCCT CCAGGAGATC TTGGTTTCAA AGGAATCCAG 
GGCCCCAGGG GTCCCCCAGG CCTGATGGGA CAAGAAGGTG TCATCGGCCC GCTGGGGATC 
CTGGGGCCTA CTGGACTCCC GGGCCCAAAA GGGGATGAGG GGAGTCGAGG TGAAACGGGA 
CTTCAAGGCC CAAGGGGTCC CCCAGGTCCC CGAGGACGCC CTGGACCGCC GGGCCCTCCA 
GGGATTCCTG TTCCATTTCA ACAAGATGAC CTCGGGACAG CTTTCCAGAC GTGGCTAGAC 
TCCTCAAACG GTGGCGTGCT CAGGACAGAG GGCTCTTCCC ATTCTGATCT GCTGGTGTTG 
GACCAGGGAG GGGAGATCTT TAAGACCCTA CACTACCTCA GCAACCTCAT CCAGAGCATC 
AAGAAGCCAC TGGGCACCAA GGAGAACCCT GCTCGCATCT GTCGAGACCT CATGAACTGT 
GAGCAGAAGA TGGCTGACGG CAAATACTGG ATCGACCCCA ACCTTGGCTG TTCTTCAGAC 
ACCATCGAAG TGACCTGTAA CTTCACCAAA GGAGGTCAAA CCTGTCTAAA ACCCATCACT 
GCCTCAAAGG TGGAGTTCGC CATCGGCCGG GTCCAGATGA ACTTCCTACA CCTCCTGAGC 
TCCGAAGTGG TCCAGCACCT GACCATCCAC TGCCTCGATG TGTCCGTGTG GCAGGAAGGG 
CAAGGCCGGC CACCCAGCAA GAAGTCTCTG CGCTTCCGGG CCTGGAACGG ACAAATCTAT 
GAGGCTGGAG GCCAGTTCCG ACCAGAGGTC TCTGTGGATG ACTGCAAGGT TCAGGACGGA 
CGCTGGCACC AAACCCGCTT CACGTTCCGG ACACAGGACC CCCAGCAGCT GCCAATCGTC 
AGCATCTACA ATCTGCCGCC CACCACCCCG GGGAAGCAGT ACCGCCTGGA AGTCGGGTCT 
GTGTGCTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918676.1
CDS	401..6235
Translation	MEPESAPVSRFRVAAPRGGIFFLWIVFCSTYCLDFTQGVPEDVDVLQQLGLGRKPKAGGSPLASHPVPQGVIPFQSGVIFTQRARVDAPTGTVIPTAFGTDLALVLSLRSHRVNNAFLFAIKSGRKKLQLGVQFIPGKIAVYVGQRRSVHFDYEVHDGQWHHLAIDIRGRAVTLFTSCGKRRVHADLHFKKDTALDPEGSFLLGKMNQHSTPFEGAVCQFDIYPSAQAAHNYCKYLKQQCRQADTYRPHLPLLEPLFPRDPSTAARPGLQTDLWLPSLRNLTTVTSRPPVVHADLARPQPTPRPSRGTAVPGSVGQPPARRPSRMTTTAPRPRAGTSLALTRTSPTRPGSTRVGLVRAASPTPRPVPRATNAKSSKTPIPASKPPGPRPARSSTKPPATLLRMAPPPSGATSGQAARSGPRPAPTPHPATKSIATSARSTSKPVPDPTAPLPGKPASPTSHAGPPSASVVPRPLSTPGTNLPPPTARTAPLVSSTRSTRTFPPILRTTAGGPRAGPRKTPLPATLPVLPGSAKMPPPAAKPSPPASPASWASTLHPTVRPVLPTSAPQTLLSPPTPDWGLPARRGPTSSKKKVTGQKAAKKAHLETTPKRVPPTKPRQKKTPADAPGDLHLTPLLQSLANLTGRAGPKPSLPRRRAPVPSAAHPTSSPPHPFPRTSGLDPRGATPVPASDYLLFDPIGPTLFPFLLGPPGQKGDTGAVGPPGLPGLPGPPGKRGPRGSPGPHGNPGRPGPPGAKGHKGDPGRSPGKAYNGEKGNAGLPGLTGQLGPVGRKGQKGYPGPSGHPGDQGEPGPAGSPGAKGYPGRQGLPGAIGDPGPKGSRGYIGLPGLFGLPGSDGERGVPGVPGQRGKMGRPGFPGDFGERGPPGTDGNPGELGAPGPPGVPGLIGDMGLLGPIGYPGPKGLKGLMGNFGEHGLKGDKGDQGLPGVPGDIGFQGDKGSQGMVGAPGVRGKPGPPGKSGDKGPAGFPGPPGPEGFPGDIGPPGENGPEGTKGKPGARGLPGPRGQSGQEGDEGPSGPPGSPGPEGHPGRKGFPGRPGPNGLKGEPGNPGRPGKVGEQGLMGFIGLVGEPGIVGEKGNRGPMGPPGVPGVKGSMGHPGTTGRTGDPGDPGNSGPPGVRGSPGMRGVKGRRGARGPDGPTGEPGSRGVKGPQGTQGKQGLTGQQGKAGERGETGPRGFPGIQGPSGPPGAKGIPGEPGPPGPQGLSGPLGEMGHKGPPGALGEPGPPGEPGMKGDFGPLGIPGEQGLIGQRGETGLEGDSGQPGPPGLKGDKGEPGPEGEKGEKGKEGLKGMEGPPGLPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGETGAPGGPGPTGTQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGFLGPIGPPGPKGEKGEQGDDGKTEGPPGPPGDRGPVGDRGDRGEHGEPGYPGQEGLVGSRGKAGQQGLPGHPGPPGQPGPKGSKGEEGPKGKQGKRGEAGSRGMQGPVGHPGPRGVVGRQGQEGLPGPDGIPGRDGRAGPQGEQGEDGEVGVMGPPGKRGNPGVGGLPGAQGPPGFKGENGLPGPPGPTGKRGTEGGTGLPGSQGEPGSKGRPGDFGEMGFPGIAGLFGPKGPPGDLGFKGIQGPRGPPGLMGQEGVIGPLGILGPTGLPGPKGDEGSRGETGLQGPRGPPGPRGRPGPPGPPGIPVPFQQDDLGTAFQTWLDSSNGGVLRTEGSSHSDLLVLDQGGEIFKTLHYLSNLIQSIKKPLGTKENPARICRDLMNCEQKMADGKYWIDPNLGCSSDTIEVTCNFTKGGQTCLKPITASKVEFAIGRVQMNFLHLLSSEVVQHLTIHCLDVSVWQEGQGRPPSKKSLRFRAWNGQIYEAGGQFRPEVSVDDCKVQDGRWHQTRFTFRTQDPQQLPIVSIYNLPPTTPGKQYRLEVGSVCFL

Target ORF information:

RefSeq Version	XM_029062843.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029062843.1

ORF Insert Sequence:

ATGGAGCCGG AGTCAGCCCC GGTCTCCAGA TTCAGAGTTG CAGCACCCAG GGGGGGAATC 
TTCTTTCTCT GGATTGTCTT CTGTTCTACC TATTGTCTGG ATTTCACCCA GGGAGTTCCT 
GAAGATGTGG ATGTCCTCCA ACAGCTGGGC CTTGGCAGGA AGCCAAAGGC AGGTGGAAGT 
CCCTTGGCAT CCCACCCTGT CCCCCAGGGG GTCATCCCGT TCCAATCAGG AGTCATCTTC 
ACCCAGCGGG CCCGGGTGGA CGCCCCCACT GGCACGGTCA TCCCTACTGC TTTTGGCACC 
GACCTGGCCC TGGTCCTGAG CCTCCGCTCC CACCGAGTCA ACAATGCCTT CCTGTTCGCC 
ATCAAGAGCG GGAGGAAGAA GCTGCAGCTG GGAGTGCAGT TCATCCCCGG CAAGATCGCC 
GTCTATGTGG GCCAACGGCG CTCTGTGCAC TTTGACTACG AGGTCCACGA TGGCCAGTGG 
CATCACCTGG CCATTGATAT CCGGGGCCGA GCGGTCACCT TATTTACTTC TTGTGGGAAG 
CGCCGAGTAC ATGCAGATCT GCATTTTAAA AAAGACACGG CACTAGACCC CGAGGGGTCG 
TTCCTCCTCG GGAAAATGAA CCAGCACTCG ACCCCATTCG AGGGCGCCGT GTGCCAGTTT 
GATATCTATC CCTCTGCCCA GGCAGCTCAT AATTACTGTA AATACCTGAA ACAACAGTGC 
CGACAGGCAG ACACCTACAG GCCCCATCTG CCCCTACTCG AGCCCCTCTT TCCCAGAGAT 
CCCAGCACGG CAGCGCGGCC GGGACTTCAG ACCGACCTCT GGCTGCCGAG TCTGAGGAAC 
CTGACCACTG TGACGTCGAG GCCGCCGGTG GTCCACGCTG ACCTCGCCCG CCCCCAGCCA 
ACCCCCAGAC CCTCCCGGGG CACGGCGGTC CCCGGGTCGG TGGGACAGCC ACCTGCCCGC 
CGGCCCTCGC GGATGACCAC GACAGCCCCT CGGCCCCGGG CAGGCACCAG CTTGGCCCTA 
ACACGAACCT CTCCCACCAG GCCGGGGAGC ACTAGGGTTG GCTTGGTGAG AGCTGCCTCT 
CCCACTCCCA GGCCAGTGCC GCGGGCAACC AATGCCAAGT CAAGCAAGAC TCCTATTCCA 
GCCAGCAAAC CCCCAGGGCC CAGACCGGCT CGCAGCTCTA CCAAACCCCC TGCTACCCTG 
CTCAGAATGG CACCGCCCCC CTCTGGCGCC ACCTCAGGTC AGGCGGCCCG CTCTGGGCCT 
CGGCCGGCCC CGACCCCCCA CCCCGCCACC AAGTCCATCG CCACCTCTGC CAGGAGCACC 
TCCAAGCCTG TCCCAGACCC CACTGCCCCC CTCCCTGGGA AGCCAGCGTC CCCGACTTCC 
CATGCTGGCC CACCCAGTGC CTCCGTAGTG CCCAGGCCTC TGAGCACCCC AGGAACCAAC 
CTCCCACCCC CCACTGCCAG GACAGCTCCC CTGGTCTCGA GCACTCGTTC CACACGGACA 
TTCCCACCCA TTCTCAGGAC AACGGCAGGA GGCCCCCGGG CTGGTCCCAG GAAAACACCT 
CTTCCCGCCA CCTTGCCAGT CCTGCCTGGC TCAGCCAAAA TGCCCCCTCC AGCTGCCAAG 
CCATCACCCC CTGCCTCCCC TGCCAGCTGG GCCAGCACCC TCCACCCCAC CGTCAGACCG 
GTGCTCCCTA CCTCTGCACC CCAAACTCTC CTGAGCCCTC CTACCCCCGA CTGGGGCCTG 
CCTGCCCGCC GGGGCCCCAC CAGCTCCAAG AAGAAGGTGA CAGGGCAGAA GGCTGCCAAG 
AAAGCCCACC TAGAGACGAC CCCCAAACGA GTCCCTCCCA CCAAGCCACG GCAGAAGAAG 
ACCCCAGCAG ACGCCCCAGG TGACCTCCAT CTCACCCCGC TGCTGCAGAG CTTGGCCAAC 
CTAACGGGTC GGGCTGGGCC CAAGCCATCC CTACCCAGAA GGAGAGCCCC AGTGCCCTCC 
GCGGCCCACC CCACCTCCAG CCCACCACAT CCATTCCCCA GGACCAGTGG CCTGGACCCC 
AGGGGAGCCA CTCCTGTCCC AGCCAGCGAC TACCTGCTCT TTGATCCCAT CGGGCCGACC 
CTCTTCCCGT TCCTGCTGGG ACCCCCAGGC CAGAAAGGAG ACACCGGAGC TGTGGGCCCC 
CCTGGACTTC CTGGGCTGCC TGGACCACCG GGAAAGAGAG GCCCCCGGGG TTCTCCTGGC 
CCTCACGGAA ACCCTGGTCG CCCCGGCCCC CCTGGTGCTA AAGGCCACAA AGGGGATCCT 
GGAAGGTCAC CTGGAAAGGC CTACAATGGG GAAAAGGGAA ATGCAGGCTT ACCTGGATTG 
ACAGGGCAGC TGGGACCAGT TGGTCGGAAG GGGCAGAAGG GCTACCCGGG ACCATCAGGG 
CATCCCGGAG ACCAGGGGGA GCCAGGACCA GCGGGAAGTC CGGGTGCCAA AGGTTATCCT 
GGCAGGCAGG GATTACCTGG AGCCATAGGA GACCCAGGAC CCAAAGGCAG CCGGGGGTAT 
ATTGGTCTCC CCGGCCTGTT CGGCCTGCCA GGATCCGACG GAGAACGAGG TGTACCCGGA 
GTGCCTGGCC AGAGGGGCAA GATGGGTAGG CCGGGCTTCC CAGGAGACTT CGGGGAAAGA 
GGACCCCCTG GAACGGATGG CAACCCCGGT GAACTGGGTG CCCCAGGCCC CCCTGGTGTT 
CCTGGGCTCA TTGGTGACAT GGGATTGTTG GGGCCAATTG GGTACCCGGG ACCCAAAGGA 
TTGAAGGGAC TGATGGGAAA CTTCGGAGAA CATGGACTGA AAGGTGATAA GGGTGACCAA 
GGGCTACCAG GGGTTCCCGG AGATATCGGA TTCCAAGGAG ACAAGGGTAG CCAGGGCATG 
GTGGGGGCAC CGGGTGTTCG AGGGAAGCCA GGGCCTCCTG GTAAGTCTGG TGACAAAGGA 
CCTGCTGGGT TTCCTGGACC TCCTGGACCT GAGGGTTTCC CGGGAGACAT TGGACCCCCA 
GGTGAAAACG GTCCAGAAGG CACCAAGGGT AAGCCAGGAG CCCGAGGTTT ACCGGGACCA 
AGAGGACAGT CCGGCCAGGA GGGAGATGAA GGACCATCGG GGCCCCCGGG ATCACCTGGC 
CCAGAGGGCC ACCCTGGACG AAAAGGGTTT CCCGGAAGGC CTGGACCCAA CGGCCTAAAG 
GGGGAGCCAG GAAATCCAGG CCGACCTGGA AAAGTAGGGG AACAGGGTTT GATGGGATTT 
ATCGGTCTTG TAGGGGAGCC AGGAATCGTG GGAGAGAAGG GTAACCGAGG GCCCATGGGA 
CCACCCGGTG TGCCTGGAGT TAAGGGGTCG ATGGGACACC CTGGGACAAC AGGCAGAACT 
GGAGACCCCG GAGATCCTGG AAATTCAGGT CCTCCTGGAG TTCGTGGGTC TCCTGGGATG 
AGAGGGGTGA AAGGTCGCCG GGGGGCCCGA GGGCCAGACG GACCGACCGG GGAGCCGGGG 
TCGCGGGGTG TGAAGGGACC GCAGGGAACC CAGGGCAAAC AGGGTCTAAC CGGACAGCAG 
GGCAAGGCAG GAGAGCGAGG AGAGACGGGA CCTCGTGGCT TCCCTGGTAT CCAGGGTCCA 
TCCGGCCCTC CCGGGGCTAA GGGAATTCCT GGGGAACCGG GCCCCCCAGG ACCCCAGGGA 
TTGTCTGGCC CTTTGGGAGA GATGGGGCAT AAGGGCCCTC CCGGTGCATT AGGAGAGCCT 
GGCCCTCCTG GGGAACCAGG GATGAAGGGT GATTTTGGAC CATTGGGCAT TCCCGGTGAA 
CAAGGTCTGA TTGGCCAGAG GGGAGAAACT GGGCTGGAAG GAGACAGTGG CCAACCTGGA 
CCTCCTGGAT TGAAGGGAGA CAAGGGAGAG CCAGGCCCTG AAGGCGAGAA AGGAGAGAAA 
GGCAAAGAGG GGCTGAAGGG CATGGAGGGC CCCCCTGGAC TTCCCGGAAT CACAGGTGTC 
CGTGGTCCCG AAGGCAAACC AGGCAAGCAA GGTGAAAAGG GCCGGACAGG ACCAAAGGGC 
GCCAAGGGGT ATCAAGGGCA CCTAGGTGAA ACTGGCGCTC CTGGAGGACC CGGGCCAACT 
GGCACACAAG GCCCTAAAGG CTCCCGAGGT ACCCTGGGAC CGACGGGAGC TCCAGGGCGG 
ATGGGAGCCC AAGGAGAACC AGGATTGCCC GGTTATGATG GACACAAAGG ATTTCTGGGA 
CCCATCGGCC CCCCAGGACC CAAAGGCGAG AAGGGTGAAC AAGGAGATGA CGGGAAGACA 
GAGGGTCCCC CTGGACCTCC TGGAGACAGG GGTCCGGTTG GTGATCGAGG AGACCGTGGA 
GAGCACGGGG AGCCGGGTTA CCCAGGCCAG GAAGGCCTTG TAGGAAGCCG AGGCAAAGCA 
GGCCAGCAAG GTCTTCCCGG GCACCCTGGA CCACCAGGCC AACCAGGACC CAAGGGATCC 
AAAGGCGAAG AGGGCCCGAA AGGGAAGCAA GGCAAGCGTG GGGAAGCCGG CAGTCGGGGG 
ATGCAGGGCC CCGTGGGGCA TCCCGGGCCA CGGGGCGTGG TGGGGAGACA AGGCCAAGAA 
GGGCTCCCCG GACCAGATGG GATTCCTGGC AGGGACGGCC GAGCGGGACC GCAGGGGGAA 
CAGGGAGAAG ACGGGGAAGT TGGTGTCATG GGTCCTCCTG GGAAGAGAGG GAATCCCGGC 
GTGGGCGGCC TGCCCGGAGC TCAGGGACCC CCAGGATTCA AGGGTGAAAA CGGGTTACCC 
GGGCCACCGG GTCCCACAGG AAAGCGAGGG ACAGAGGGAG GAACGGGGCT TCCCGGAAGC 
CAGGGGGAGC CAGGATCCAA GGGCCGGCCG GGGGACTTTG GCGAGATGGG CTTCCCGGGG 
ATCGCAGGCC TGTTTGGCCC CAAGGGGCCT CCAGGAGATC TTGGTTTCAA AGGAATCCAG 
GGCCCCAGGG GTCCCCCAGG CCTGATGGGA CAAGAAGGTG TCATCGGCCC GCTGGGGATC 
CTGGGGCCTA CTGGACTCCC GGGCCCAAAA GGGGATGAGG GGAGTCGAGG TGAAACGGGA 
CTTCAAGGCC CAAGGGGTCC CCCAGGTCCC CGAGGACGCC CTGGACCGCC GGGCCCTCCA 
GGGATTCCTG TTCCATTTCA ACAAGATGAC CTCGGGACAG CTTTCCAGAC GTGGCTAGAC 
TCCTCAAACG GTGGCGTGCT CAGGACAGAG GGCTCTTCCC ATTCTGATCT GCTGGTGTTG 
GACCAGGGAG GGGAGATCTT TAAGACCCTA CACTACCTCA GCAACCTCAT CCAGAGCATC 
AAGAAGCCAC TGGGCACCAA GGAGAACCCT GCTCGCATCT GTCGAGACCT CATGAACTGT 
GAGCAGAAGA TGGCTGACGG CAAATACTGG ATCGACCCCA ACCTTGGCTG TTCTTCAGAC 
ACCATCGAAG TGACCTGTAA CTTCACCAAA GGAGGTCAAA CCTGTCTAAA ACCCATCACT 
GCCTCAAAGG TGGAGTTCGC CATCGGCCGG GTCCAGATGA ACTTCCTACA CCTCCTGAGC 
TCCGAAGTGG TCCAGCACCT GACCATCCAC TGCCTCGATG TGTCCGTGTG GCAGGAAGGG 
CAAGGCCGGC CACCCAGCAA GAAGTCTCTG CGCTTCCGGG CCTGGAACGG ACAAATCTAT 
GAGGCTGGAG GCCAGTTCCG ACCAGAGGTC TCTGTGGATG ACTGCAAGGT TCAGGACGGA 
CGCTGGCACC AAACCCGCTT CACGTTCCGG ACACAGGACC CCCAGCAGCT GCCAATCGTC 
AGCATCTACA ATCTGCCGCC CACCACCCCG GGGAAGCAGT ACCGCCTGGA AGTCGGGTCT 
GTGTGCTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL27A1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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