Dock8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dock8
Entrez Gene ID	76088
Full Name	dedicator of cytokinesis 8
Synonyms	1200017A24Rik,5830472H07Rik,A130095G14Rik,AI461977
General protein information	Preferred Names dedicator of cytokinesis 8 Names dedicator of cytokinesis protein 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247397.3	XP_011245699.1	dedicator of cytokinesis protein 8 isoform X1
XM_011247397.2	XP_011245699.1	dedicator of cytokinesis protein 8 isoform X1
XM_006527435.3	XP_006527498.1	dedicator of cytokinesis protein 8 isoform X1
XM_006527436.3	XP_006527499.1	dedicator of cytokinesis protein 8 isoform X2
XM_006527436.4	XP_006527499.1	dedicator of cytokinesis protein 8 isoform X2
NM_028785.3	NP_083061.2	dedicator of cytokinesis protein 8

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dock8	XP_001920107.4
Bos taurus (cattle)	DOCK8	NP_001178461.1
Homo sapiens (human)	DOCK8	NP_982272.2
Pan troglodytes (chimpanzee)	DOCK8	XP_003312029.1
Xenopus tropicalis (tropical clawed frog)	dock8	XP_002935647.2
Canis lupus familiaris (dog)	DOCK8	XP_005615946.1
Gallus gallus (chicken)	DOCK8	XP_424926.4
Macaca mulatta (Rhesus monkey)	LOC698081	XP_002808151.1
Rattus norvegicus (Norway rat)	Dock8	NP_001032882.2
Mus musculus (house mouse)	Dock8	NP_083061.2

Gene Ontology
Bibliography

Related articles in PubMed

IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α
Liu D, Yin X, Olyha SJ, Nascimento MSL, Chen P, White T, Gowthaman U, Zhang T, Gertie JA, Zhang B, Xu L, Yurieva M, Devine L, Williams A, Eisenbarth SC
Immunity51(1)64-76.e7(2019 07)

Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery.
Wilson AS, Law HD, Knobbe-Thomsen CB, Kearney CJ, Oliaro J, Binsfeld C, Burgio G, Starrs L, Brenner D, Randall KL, Brüstle A
European journal of immunology49(5)770-781(2019 May)

WASP-mediated regulation of anti-inflammatory macrophages is IL-10 dependent and is critical for intestinal homeostasis.
Biswas A, Shouval DS, Griffith A, Goettel JA, Field M, Kang YH, Konnikova L, Janssen E, Redhu NS, Thrasher AJ, Chatila T, Kuchroo VK, Geha RS, Notarangelo LD, Pai SY, Horwitz BH, Snapper SB
Nature communications9(1)1779(2018 05)

Migratory CD11b
Krishnaswamy JK, Gowthaman U, Zhang B, Mattsson J, Szeponik L, Liu D, Wu R, White T, Calabro S, Xu L, Collet MA, Yurieva M, Alsén S, Fogelstrand P, Walter A, Heath WR, Mueller SN, Yrlid U, Williams A, Eisenbarth SC
Science immunology2(18)(2017 12)

An Anti-Inflammatory Role for NLRP10 in Murine Cutaneous Leishmaniasis.
Clay GM, Valadares DG, Graff JW, Ulland TK, Davis RE, Scorza BM, Zhanbolat BS, Chen Y, Sutterwala FS, Wilson ME
Journal of immunology (Baltimore, Md. : 1950)199(8)2823-2833(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dock8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dock8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55802	XM_006527436.4 Latest version!	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu06123	NM_028785.3 Latest version!	Mus musculus dedicator of cytokinesis 8 (Dock8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu55801	XM_011247397.3 Latest version!	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu55801	XM_006527435.3 Latest version!	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMu55801	XM_011247397.2	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu55802	XM_006527436.3	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dock8 ( XM_006527436.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527499.1 Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55802
Clone ID Related Accession (Same CDS sequence)	XM_006527436.3 , XM_006527436.4
Accession Version	XM_006527436.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCAAGTC AGTCTGAGGA AACTGAATTT TCTCAGGAGC GTGACTTGCT CCAGTTAACA 
GAGAAATCAC AGGACTGTGC ATTTAATCTT GGATCAGGGC ATCCAGAGTT TGAGACGGGG 
ACGATGGCTT CATGTGCGAG TGTGAGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527499.1
CDS	141..3533
Translation	MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSASQSEETEFSQERDLLQLTEKSQDCAFNLGSGHPEFETGTMASCASVRT

Target ORF information:

RefSeq Version	XM_006527436.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527436.3

ORF Insert Sequence:

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCAAGTC AGTCTGAGGA AACTGAATTT TCTCAGGAGC GTGACTTGCT CCAGTTAACA 
GAGAAATCAC AGGACTGTGC ATTTAATCTT GGATCAGGGC ATCCAGAGTT TGAGACGGGG 
ACGATGGCTT CATGTGCGAG TGTGAGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_006527436.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527499.1 Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55802
Clone ID Related Accession (Same CDS sequence)	XM_006527436.3 , XM_006527436.4
Accession Version	XM_006527436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527436.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCAAGTC AGTCTGAGGA AACTGAATTT TCTCAGGAGC GTGACTTGCT CCAGTTAACA 
GAGAAATCAC AGGACTGTGC ATTTAATCTT GGATCAGGGC ATCCAGAGTT TGAGACGGGG 
ACGATGGCTT CATGTGCGAG TGTGAGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527499.1
CDS	331..3723
Translation	MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSASQSEETEFSQERDLLQLTEKSQDCAFNLGSGHPEFETGTMASCASVRT

Target ORF information:

RefSeq Version	XM_006527436.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527436.4

ORF Insert Sequence:

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCAAGTC AGTCTGAGGA AACTGAATTT TCTCAGGAGC GTGACTTGCT CCAGTTAACA 
GAGAAATCAC AGGACTGTGC ATTTAATCTT GGATCAGGGC ATCCAGAGTT TGAGACGGGG 
ACGATGGCTT CATGTGCGAG TGTGAGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( NM_028785.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083061.2 Mus musculus dedicator of cytokinesis 8 (Dock8), mRNA.

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CloneID	OMu06123
Clone ID Related Accession (Same CDS sequence)	NM_028785.3
Accession Version	NM_028785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK050543.1, DT909592.1, AK038285.1, AK131180.1 and AK028968.1. On Apr 6, 2005 this sequence version replaced NM_028785.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCCAAGC TGAATATCCT TCCAACGCTC ATCTCCATGC GGCTGGAATT CCTGAGGATC 
CTCTGCAGCC ATGAGCACTA CCTCAACTTG AACCTCCTCT TCATGAATAC CGACACCGCA 
CCAGCATCTC CCTGCCCCTC CATATCCTCC CAGAACTCGA GTTCCTGCTC CAGTTTCCAG 
GACCAAAAGA TTGCCAGCAT GTTCGATCTG ACCCCGGAGT ACCGGCAGCA GCACTTCCTT 
ACAGGGCTGC TCTTCACGGA GCTGGCTGTT GCCCTGGATG CTGAGGGGGA TGGAATTAGC 
AGAGTACAGA GAAAAGCCGT GAGTGCCATC CACAGCCTTC TGTGTTCTCA CGACCTGGAT 
CCACGGTGTC GCAAACCGGA AGTGAAAGTC AAAATCGCCG CCCTTTACCT GCCGTTGGTC 
GGCATCATTC TGGACGCTCT GCCACAGCTC TATGACTTTA CAGATGCTCG CAGTGGAAGG 
AGTCGTGCCA GTGGCTCGTA TGAAGAACAA GATGTGGCCA ACGGAATCAA CCAGAATGTG 
GCCCTGGCCA TAGCGGGGAA TCACTTTAAT TTGAAGACCA GTGGAGCAAT GCTGTCTTCC 
TTGCCCTATA AGCAGTACAA CATGCTGAAT GCAGACACCA CCCGCCACCT CATGATTTGC 
TTCCTGTGGA TCATGAAAAA TGCTGATCAG AGCCTCATCA GGAAGTGGAT CGCTGACCTG 
CCTTCCATGC AGCTCAATAG GATTCTGGAC CTGCTGTTCA TCTGTGTCTC CTGCTTTGAA 
TACAAGGGAA AGCAGAGTTC TGACAAAGTC AGTAACCAGG TCCTGCAGAA GTCAAGAGAC 
GTCAAGGCCA AGCTGGAAGA GGCCCTGCTC CGTGGGGAAG GAGCCCGTGG GGAGATGATG 
CGCCGTCGCA TTCCAGGGAC TGACCGGTTT CCAGGCATAA ATGAAAATCT GAGATGGAGG 
AAAGAGCAGA CACAGTGGCG GCAGGCTAAT GAGAAGCTGG ACAAAACAAA GGCAGAGTTA 
GATCAAGAAG CCTTGATCAG TGGCAACCTG GCTACAGAAG CTAATTTGAT CATCCTGGAT 
ATGCAGGAGA ACATCATCCA GGCAAGCTCC GCCCTGGACT GTAAAGACAG CCTGCTGGGA 
GGTGTCCTCC GGGTCCTGGT GAATTCTCTG AGCTGTGACC AGAGCACCAC CTACCTGACT 
CACTGTTTCG CAACCCTCCG AGCCCTCATC GCCAAGTTTG GAGACCTGCT GTTCGAGGAG 
GAGATGGAGC AGTGTGCTGA CCTGTGTCAG CGGGTGCTAC ATCACTGCAG CAGCAGCATG 
GACGTCACAC GGAGCCAAGC CTGCGCCACC CTCTACCTCC TCATGCGGTT CAGCTTCGGA 
GCCACCAGTA ACTTTGCAAG GGTAAAGATG CAAGTGACCA TGGCACTGGC ATCCCTGGTA 
GGCAAAGCAC CAGACTTCAA CGAAGAGCAC CTGAGAAGGT CCTTAAGGAC AATTTTGGCC 
TATTCAGAAG AGGACACGGC CATGCAGACA ACTCCTTTTC CCATGCAGGT GGAGGAACTT 
CTCTGCAATC TGAACAGCAT TCTGTACGAC ACAGTGAAGA TGAGGGAATT CCAGGAAGAC 
CCTGAGATGC TTATGGACCT CATGTACAGA ATTGCCAAGA GCTACCAGGC ATCGCCTGAC 
CTGCGGCTGA CTTGGCTCCA GAACATGGCA GAGAAACACA CTAAGAAGAA GTGCTTCACA 
GAGGCCGCCA TGTGCCTGGT GCATGCAGCC GCCCTGGTGG CCGAGTACCT GAGCATGCTG 
GAGGACCACA GCTACCTGCC GGTGGGCAGC GTCAGCTTTC AGAATATTTC TTCCAATGTG 
CTTGAGGAGT CTGCAGTCTC TGATGACACC TTGTCACCTG ATGAGGACGG CGTATGCTCT 
GGTCGGTACT TCACTGAGAG TGGCCTGGTG GGCCTCCTGG AGCAGGCTGC GGAGCTCTTC 
AGCACGGGAG GCTTGTACGA GACGGTTAAT GAAGTCTACA AGCTGGTCAT CCCTATCCTG 
GAGGCACACA GAGATTTCCG GAAGCTGACC TCCACTCACG ACAAGCTGCA GAAGGCCTTC 
GATAACATCA TCAACAAGGA CCATAAGAGG ATGTTTGGGA CCTACTTCCG AGTTGGTTTC 
TACGGATCCC GATTTGGGGA TTTGGATGAG CAGGAGTTCG TGTACAAGGA ACCCGCAATC 
ACGAAGCTCC CGGAGATCTC ACATAGACTA GAGGGATTTT ATGGCCAGTG TTTCGGTGCA 
GAGTTTGTGG AAGTGATAAA AGACTCTACT CCAGTGGACA AAACCAAGTT GGATCCTAAC 
AAGGCCTACA TTCAGATCAC TTTTGTGGAG CCTTACTTTG ATGAATATGA GATGAAAGAC 
CGGGTGACCT ACTTCGAGAA GAATTTCAAC CTCCGGAGGT TCATGTACAC CACCCCCTTC 
ACCCTGGAGG GGAGACCCCG GGGCGAGCTT CATGAGCAAC ACCGCAGAAA CACCGTGCTC 
ACCACCATGC ACGCCTTCCC CTACATCAAG ACCAGGATCC GAGTCAGCCA GAAAGAGGAG 
TTCGTTTTGA CTCCGATTGA GGTTGCCATT GAAGATATGA AGAAGAAGAC CCTGCAGTTA 
GCCGTGGCCA CTCACCAGGA GCCCCCTGAT GCAAAGATGC TGCAAATGGT ACTGCAGGGC 
TCTGTAGGAG CCACTGTAAA TCAGGGACCA CTGGAGGTGG CCCAAGTGTT CTTGGCTGAA 
ATTCCAGCTG ACCCAAAGCT CTACCGACAT CACAACAAGC TGAGGTTGTG CTTCAAGGAG 
TTCATAATGC GATGCGGAGA GGCCGTGGAG AAGAACAGGC GACTCATCAC CGCAGAGCAG 
CGGGAGTACC AGCAGGAGCT GAAGAAGAAC TACAACAAGC TGAGAGACAG CCTCAGGCCC 
ATGATTGAGC GGAAAATCCC AGAGCTCTAC AAGCCCATAT TCAGAGTTGA CAGTCAGAAG 
AGGGACTCTT TCCACAGATC TAGTTTCAGG AAATGTGAAA CCCAGTTGTC ACAGGGCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083061.2
CDS	123..6425
Translation	MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	NM_028785.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028785.3

ORF Insert Sequence:

ATGGCCACGC TGCCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCGTCAGAAA TAAGGAAGCA GTTTACGCTC CCACCCAACC TCGGACAGTA CCATCGGCAC 
AGTATCAGTA CATCTGGTTT CCCCTCTCTT CAGCTACCTC AGCTTTATGA GCCTGTCGAG 
CCAGTGGACT TTGAAGGACT CGTGATGACA CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAAGAATGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGAGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTAAACC GGAAAAACCA AGGAAGTTCA 
GAGTTTTGCA GCTTTAAAAA GACGGGATCT CGCAGAGATT TTCAGAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG ACACTCAGAC AGGACCCCGT 
CATCCCTTAA ACGTGCTGTG TGACGTGTCT GGGAAGGGCC CCCTCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGATGAGCGG CTGGAAAACC TGCTCCAGCT TGTGAGCGCT 
GAGGACTTTG AGAAGGAGAA GGAGGAGGCC CGCAAGACCA ATCGGCCGGC TGAGCTCTTT 
GCCCTCTATC CGCCCGTGGA TGAGGAGGAT GCTGTGGAAA TACGTCCAGT ACCTGAATGT 
CCTAAGGAAC ATCTGGGCAA CAGAATATTG GTCAAGGTGC TGACTCTGAA GTTTGAGATT 
GAAATTGAAC CTCTGTTTGC CAGTATTGCC CTCTATGACG TTAAAGAAAG GAAAAAGATC 
TCAGAAAATT TCCACTGTGA CCTGAACTCC GACCAGTTCA AAGGGTTTCT GCGAGCTCAC 
ACACCCTCGA TTGACCCATC GAGTCAGGCT AGGTCTGCCG TGTTCTCTGT CACCTACCCA 
TCTTCAGACA TCTACCTGGT TGTCAAGATT GAAAAGGTCC TTCAGCAAGG AGAGATTGCA 
GACTGTGCAG AACCCTACAT GATCATCAAA GAAAGCGATG GTGGAAAGAG TAAAGAAAAG 
GTTGAAAAAC TAAAACTTCA AGCTGAATCC TTCTGCCAAC GTTTGGGGAA ATACCGGATG 
CCCTTCGCCT GGGCCCCCAT TAGCTTAGCA AGCTTCTTCA ACATCTCCAC CCTTGAAAGG 
GAGAGCACAG ATGTGGAGCC TGGGGTTGGG AGGAACTCTG TGGGTGAGAA GAGGAGCTTG 
TCCCAATCCA GGAGGCCCTC TGAGCGAACC CTCTCCTTGG AGGAAAATGG AGTTGGATCC 
AACTTCAAAG CCACCACCTT GGCCACCAAC ATCTTCTTCA AACAGGAGGG AGATCGCCTT 
AGTGATGAAG ACTTGTTCAA GTTTTTAGCT GACTACAAGA GATCTTCATC CCTACAGCGA 
AGAGTCAAAT CCATCCCAGG CTCACTGAGG CTGGAGATAT CCCCAGCTCC CGACGTGATG 
AACTGCTGCC TGACGCCCGA GATGCTGCCA GTCAAACCTT TTCCTGAAAA TCGGACGCGT 
CCACACAAGG AGATTTTGGA ATTTCCGATC CGGGAGGTGT ACGTCCCTCA CACTGTGTAC 
AGAAACCTTC TGTACGTATA CCCCCAGCGA CTGAACTTCG CTAGCAAGCT AGCATCTGCC 
CGGAACATCA CAATAAAGAT TCAGTTTATG TGCGGAGAAG ACCCCAGCAA TGCTATGCCG 
GTCATCTTTG GCAAGTCCAG TGGGCCTGAA TTTCTGCAGG AAGTATATAC AGCTATTACA 
TACCATAATA AGTCTCCTGA CTTTTACGAA GAAGTGAAAA TTAAGCTCCC TGCCAAGCTC 
ACAGTGAATC ATCACCTCCT CTTCACCTTC TACCACATCA GCTGTCAGCA GAAGCAAGGG 
GCCTCCGGAG AAAGCCTTCT GGGGTACTCG TGGCTGCCGA TTCTGTTAAA CGAACGTCTT 
CAAACCGGAT CCTACTGTCT GCCTGTTGCC TTGGAAAAAC TACCACCCAA CTACTCCATA 
CATTCTGCTG AGAAAGTCCC TTTACAGAAT CCTCCCATTA AGTGGGCCGA GGGCCATAAG 
GGGGTATTTA ATATTGAAGT GCAAGCTGTT TCTTCCGTCC ACACCCAGGA TAACCACCTG 
GAGAAGTTCT TCACCCTTTG CCACTCCCTG GAGAGCCAGG TGAGCTTCCC TATCCGTGTG 
CTGGACCAGA AGATCACCGA GAGCACGCTG GAGCACGAGC TGAAACTCAG CATCATCTGC 
CTCAACTCCT CCCGCCTGGA GCCCCTCGTG CTCTTCCTCC ACCTGGTGCT TGACAAGCTG 
TTCCAGCTTT CCGTGCAGCC CATGGTCATT GCTGGCCAAA CAGCAAACTT CTCCCAGTTT 
GCCTTCGAGT CTGTGGTGGC CATTGCCAAT AGCCTGCACA ACAGCAAGGA CCTGAGGAAG 
GACCAGCACG GGAGGAACTG CCTTCTGGCC TCCTACGTGC ACTACGTGTT CCGGCTGCCG 
GAACTGCACA GGGATACATC CAAGTCAGGT GGCCCCACCA CCGTAGTCCC CGACCCCCGA 
TACCACACAT ATGGACGCAC ATCTGCCGCT GCAGTGAGTT CAAAGCTGAT GCAGGCCCGT 
GTGATGAGCA GCAGCAACCC AGACCTGACT GGGTCACACT GTGCAGCCGA TGAGGAAGTT 
AAGAACATCA TGTCTTCAAA GATTGCCGAT CGCAACTGCA GCCGGATGTC TTACTATTGC 
TCTGGCAATA GTGATGCGCC AGGTTCAACT GCAGCCCCAA GACCAGTCAG CAAAAAGCAT 
TTCCATGAGG AGCTTGCCCT GCAGATGGTG GTCAGCACTG GAGTAGTGAG AGAAACGGTC 
TTCAAGTACG CCTGGTTCTT CTTTGAGCTT CTGGTGAAAA GCATGGCACA GTATGTCCAT 
AACCTGGATA AACGGGACAG TTTTCGGAGG ACTCGTTTTT CTGACCGCTT CAAAGATGAC 
ATAACTACCA TTGTTAATGT GGTCACCTCG GAGATAGCAG CCCTTTTAGT GAAACCTCAG 
AAGGAAAGCG AGCAGGCAGA AAAGATCAAC ATCAGCCTTG CCTTCTTCCT GTATGACCTC 
CTGTCAATCA TGGACAGAGG CTTCGTGTTC AACCTCATCA AGCATTACTG CAGCCAGCTG 
TCAGCCAAGC TGAATATCCT TCCAACGCTC ATCTCCATGC GGCTGGAATT CCTGAGGATC 
CTCTGCAGCC ATGAGCACTA CCTCAACTTG AACCTCCTCT TCATGAATAC CGACACCGCA 
CCAGCATCTC CCTGCCCCTC CATATCCTCC CAGAACTCGA GTTCCTGCTC CAGTTTCCAG 
GACCAAAAGA TTGCCAGCAT GTTCGATCTG ACCCCGGAGT ACCGGCAGCA GCACTTCCTT 
ACAGGGCTGC TCTTCACGGA GCTGGCTGTT GCCCTGGATG CTGAGGGGGA TGGAATTAGC 
AGAGTACAGA GAAAAGCCGT GAGTGCCATC CACAGCCTTC TGTGTTCTCA CGACCTGGAT 
CCACGGTGTC GCAAACCGGA AGTGAAAGTC AAAATCGCCG CCCTTTACCT GCCGTTGGTC 
GGCATCATTC TGGACGCTCT GCCACAGCTC TATGACTTTA CAGATGCTCG CAGTGGAAGG 
AGTCGTGCCA GTGGCTCGTA TGAAGAACAA GATGTGGCCA ACGGAATCAA CCAGAATGTG 
GCCCTGGCCA TAGCGGGGAA TCACTTTAAT TTGAAGACCA GTGGAGCAAT GCTGTCTTCC 
TTGCCCTATA AGCAGTACAA CATGCTGAAT GCAGACACCA CCCGCCACCT CATGATTTGC 
TTCCTGTGGA TCATGAAAAA TGCTGATCAG AGCCTCATCA GGAAGTGGAT CGCTGACCTG 
CCTTCCATGC AGCTCAATAG GATTCTGGAC CTGCTGTTCA TCTGTGTCTC CTGCTTTGAA 
TACAAGGGAA AGCAGAGTTC TGACAAAGTC AGTAACCAGG TCCTGCAGAA GTCAAGAGAC 
GTCAAGGCCA AGCTGGAAGA GGCCCTGCTC CGTGGGGAAG GAGCCCGTGG GGAGATGATG 
CGCCGTCGCA TTCCAGGGAC TGACCGGTTT CCAGGCATAA ATGAAAATCT GAGATGGAGG 
AAAGAGCAGA CACAGTGGCG GCAGGCTAAT GAGAAGCTGG ACAAAACAAA GGCAGAGTTA 
GATCAAGAAG CCTTGATCAG TGGCAACCTG GCTACAGAAG CTAATTTGAT CATCCTGGAT 
ATGCAGGAGA ACATCATCCA GGCAAGCTCC GCCCTGGACT GTAAAGACAG CCTGCTGGGA 
GGTGTCCTCC GGGTCCTGGT GAATTCTCTG AGCTGTGACC AGAGCACCAC CTACCTGACT 
CACTGTTTCG CAACCCTCCG AGCCCTCATC GCCAAGTTTG GAGACCTGCT GTTCGAGGAG 
GAGATGGAGC AGTGTGCTGA CCTGTGTCAG CGGGTGCTAC ATCACTGCAG CAGCAGCATG 
GACGTCACAC GGAGCCAAGC CTGCGCCACC CTCTACCTCC TCATGCGGTT CAGCTTCGGA 
GCCACCAGTA ACTTTGCAAG GGTAAAGATG CAAGTGACCA TGGCACTGGC ATCCCTGGTA 
GGCAAAGCAC CAGACTTCAA CGAAGAGCAC CTGAGAAGGT CCTTAAGGAC AATTTTGGCC 
TATTCAGAAG AGGACACGGC CATGCAGACA ACTCCTTTTC CCATGCAGGT GGAGGAACTT 
CTCTGCAATC TGAACAGCAT TCTGTACGAC ACAGTGAAGA TGAGGGAATT CCAGGAAGAC 
CCTGAGATGC TTATGGACCT CATGTACAGA ATTGCCAAGA GCTACCAGGC ATCGCCTGAC 
CTGCGGCTGA CTTGGCTCCA GAACATGGCA GAGAAACACA CTAAGAAGAA GTGCTTCACA 
GAGGCCGCCA TGTGCCTGGT GCATGCAGCC GCCCTGGTGG CCGAGTACCT GAGCATGCTG 
GAGGACCACA GCTACCTGCC GGTGGGCAGC GTCAGCTTTC AGAATATTTC TTCCAATGTG 
CTTGAGGAGT CTGCAGTCTC TGATGACACC TTGTCACCTG ATGAGGACGG CGTATGCTCT 
GGTCGGTACT TCACTGAGAG TGGCCTGGTG GGCCTCCTGG AGCAGGCTGC GGAGCTCTTC 
AGCACGGGAG GCTTGTACGA GACGGTTAAT GAAGTCTACA AGCTGGTCAT CCCTATCCTG 
GAGGCACACA GAGATTTCCG GAAGCTGACC TCCACTCACG ACAAGCTGCA GAAGGCCTTC 
GATAACATCA TCAACAAGGA CCATAAGAGG ATGTTTGGGA CCTACTTCCG AGTTGGTTTC 
TACGGATCCC GATTTGGGGA TTTGGATGAG CAGGAGTTCG TGTACAAGGA ACCCGCAATC 
ACGAAGCTCC CGGAGATCTC ACATAGACTA GAGGGATTTT ATGGCCAGTG TTTCGGTGCA 
GAGTTTGTGG AAGTGATAAA AGACTCTACT CCAGTGGACA AAACCAAGTT GGATCCTAAC 
AAGGCCTACA TTCAGATCAC TTTTGTGGAG CCTTACTTTG ATGAATATGA GATGAAAGAC 
CGGGTGACCT ACTTCGAGAA GAATTTCAAC CTCCGGAGGT TCATGTACAC CACCCCCTTC 
ACCCTGGAGG GGAGACCCCG GGGCGAGCTT CATGAGCAAC ACCGCAGAAA CACCGTGCTC 
ACCACCATGC ACGCCTTCCC CTACATCAAG ACCAGGATCC GAGTCAGCCA GAAAGAGGAG 
TTCGTTTTGA CTCCGATTGA GGTTGCCATT GAAGATATGA AGAAGAAGAC CCTGCAGTTA 
GCCGTGGCCA CTCACCAGGA GCCCCCTGAT GCAAAGATGC TGCAAATGGT ACTGCAGGGC 
TCTGTAGGAG CCACTGTAAA TCAGGGACCA CTGGAGGTGG CCCAAGTGTT CTTGGCTGAA 
ATTCCAGCTG ACCCAAAGCT CTACCGACAT CACAACAAGC TGAGGTTGTG CTTCAAGGAG 
TTCATAATGC GATGCGGAGA GGCCGTGGAG AAGAACAGGC GACTCATCAC CGCAGAGCAG 
CGGGAGTACC AGCAGGAGCT GAAGAAGAAC TACAACAAGC TGAGAGACAG CCTCAGGCCC 
ATGATTGAGC GGAAAATCCC AGAGCTCTAC AAGCCCATAT TCAGAGTTGA CAGTCAGAAG 
AGGGACTCTT TCCACAGATC TAGTTTCAGG AAATGTGAAA CCCAGTTGTC ACAGGGCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_011247397.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245699.1 Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

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CloneID	OMu55801
Clone ID Related Accession (Same CDS sequence)	XM_011247397.3 , XM_011247397.2 , XM_006527435.3
Accession Version	XM_011247397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245699.1
CDS	318..6416
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_011247397.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247397.3

ORF Insert Sequence:

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_011247397.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245699.1 Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

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CloneID	OMu55801
Clone ID Related Accession (Same CDS sequence)	XM_011247397.3 , XM_011247397.2 , XM_006527435.3
Accession Version	XM_011247397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245699.1
CDS	318..6416
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_011247397.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247397.2

ORF Insert Sequence:

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_006527435.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527498.1 Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.

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CloneID	OMu55801
Clone ID Related Accession (Same CDS sequence)	XM_011247397.3 , XM_011247397.2 , XM_006527435.3
Accession Version	XM_006527435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527498.1
CDS	123..6221
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_006527435.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527435.3

ORF Insert Sequence:

ATGACACACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAA GAATGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGAGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TAAACCGGAA AAACCAAGGA AGTTCAGAGT TTTGCAGCTT TAAAAAGACG 
GGATCTCGCA GAGATTTTCA GAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGACAC TCAGACAGGA CCCCGTCATC CCTTAAACGT GCTGTGTGAC 
GTGTCTGGGA AGGGCCCCCT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAT 
GAGCGGCTGG AAAACCTGCT CCAGCTTGTG AGCGCTGAGG ACTTTGAGAA GGAGAAGGAG 
GAGGCCCGCA AGACCAATCG GCCGGCTGAG CTCTTTGCCC TCTATCCGCC CGTGGATGAG 
GAGGATGCTG TGGAAATACG TCCAGTACCT GAATGTCCTA AGGAACATCT GGGCAACAGA 
ATATTGGTCA AGGTGCTGAC TCTGAAGTTT GAGATTGAAA TTGAACCTCT GTTTGCCAGT 
ATTGCCCTCT ATGACGTTAA AGAAAGGAAA AAGATCTCAG AAAATTTCCA CTGTGACCTG 
AACTCCGACC AGTTCAAAGG GTTTCTGCGA GCTCACACAC CCTCGATTGA CCCATCGAGT 
CAGGCTAGGT CTGCCGTGTT CTCTGTCACC TACCCATCTT CAGACATCTA CCTGGTTGTC 
AAGATTGAAA AGGTCCTTCA GCAAGGAGAG ATTGCAGACT GTGCAGAACC CTACATGATC 
ATCAAAGAAA GCGATGGTGG AAAGAGTAAA GAAAAGGTTG AAAAACTAAA ACTTCAAGCT 
GAATCCTTCT GCCAACGTTT GGGGAAATAC CGGATGCCCT TCGCCTGGGC CCCCATTAGC 
TTAGCAAGCT TCTTCAACAT CTCCACCCTT GAAAGGGAGA GCACAGATGT GGAGCCTGGG 
GTTGGGAGGA ACTCTGTGGG TGAGAAGAGG AGCTTGTCCC AATCCAGGAG GCCCTCTGAG 
CGAACCCTCT CCTTGGAGGA AAATGGAGTT GGATCCAACT TCAAAGCCAC CACCTTGGCC 
ACCAACATCT TCTTCAAACA GGAGGGAGAT CGCCTTAGTG ATGAAGACTT GTTCAAGTTT 
TTAGCTGACT ACAAGAGATC TTCATCCCTA CAGCGAAGAG TCAAATCCAT CCCAGGCTCA 
CTGAGGCTGG AGATATCCCC AGCTCCCGAC GTGATGAACT GCTGCCTGAC GCCCGAGATG 
CTGCCAGTCA AACCTTTTCC TGAAAATCGG ACGCGTCCAC ACAAGGAGAT TTTGGAATTT 
CCGATCCGGG AGGTGTACGT CCCTCACACT GTGTACAGAA ACCTTCTGTA CGTATACCCC 
CAGCGACTGA ACTTCGCTAG CAAGCTAGCA TCTGCCCGGA ACATCACAAT AAAGATTCAG 
TTTATGTGCG GAGAAGACCC CAGCAATGCT ATGCCGGTCA TCTTTGGCAA GTCCAGTGGG 
CCTGAATTTC TGCAGGAAGT ATATACAGCT ATTACATACC ATAATAAGTC TCCTGACTTT 
TACGAAGAAG TGAAAATTAA GCTCCCTGCC AAGCTCACAG TGAATCATCA CCTCCTCTTC 
ACCTTCTACC ACATCAGCTG TCAGCAGAAG CAAGGGGCCT CCGGAGAAAG CCTTCTGGGG 
TACTCGTGGC TGCCGATTCT GTTAAACGAA CGTCTTCAAA CCGGATCCTA CTGTCTGCCT 
GTTGCCTTGG AAAAACTACC ACCCAACTAC TCCATACATT CTGCTGAGAA AGTCCCTTTA 
CAGAATCCTC CCATTAAGTG GGCCGAGGGC CATAAGGGGG TATTTAATAT TGAAGTGCAA 
GCTGTTTCTT CCGTCCACAC CCAGGATAAC CACCTGGAGA AGTTCTTCAC CCTTTGCCAC 
TCCCTGGAGA GCCAGGTGAG CTTCCCTATC CGTGTGCTGG ACCAGAAGAT CACCGAGAGC 
ACGCTGGAGC ACGAGCTGAA ACTCAGCATC ATCTGCCTCA ACTCCTCCCG CCTGGAGCCC 
CTCGTGCTCT TCCTCCACCT GGTGCTTGAC AAGCTGTTCC AGCTTTCCGT GCAGCCCATG 
GTCATTGCTG GCCAAACAGC AAACTTCTCC CAGTTTGCCT TCGAGTCTGT GGTGGCCATT 
GCCAATAGCC TGCACAACAG CAAGGACCTG AGGAAGGACC AGCACGGGAG GAACTGCCTT 
CTGGCCTCCT ACGTGCACTA CGTGTTCCGG CTGCCGGAAC TGCACAGGGA TACATCCAAG 
TCAGGTGGCC CCACCACCGT AGTCCCCGAC CCCCGATACC ACACATATGG ACGCACATCT 
GCCGCTGCAG TGAGTTCAAA GCTGATGCAG GCCCGTGTGA TGAGCAGCAG CAACCCAGAC 
CTGACTGGGT CACACTGTGC AGCCGATGAG GAAGTTAAGA ACATCATGTC TTCAAAGATT 
GCCGATCGCA ACTGCAGCCG GATGTCTTAC TATTGCTCTG GCAATAGTGA TGCGCCAGGT 
TCAACTGCAG CCCCAAGACC AGTCAGCAAA AAGCATTTCC ATGAGGAGCT TGCCCTGCAG 
ATGGTGGTCA GCACTGGAGT AGTGAGAGAA ACGGTCTTCA AGTACGCCTG GTTCTTCTTT 
GAGCTTCTGG TGAAAAGCAT GGCACAGTAT GTCCATAACC TGGATAAACG GGACAGTTTT 
CGGAGGACTC GTTTTTCTGA CCGCTTCAAA GATGACATAA CTACCATTGT TAATGTGGTC 
ACCTCGGAGA TAGCAGCCCT TTTAGTGAAA CCTCAGAAGG AAAGCGAGCA GGCAGAAAAG 
ATCAACATCA GCCTTGCCTT CTTCCTGTAT GACCTCCTGT CAATCATGGA CAGAGGCTTC 
GTGTTCAACC TCATCAAGCA TTACTGCAGC CAGCTGTCAG CCAAGCTGAA TATCCTTCCA 
ACGCTCATCT CCATGCGGCT GGAATTCCTG AGGATCCTCT GCAGCCATGA GCACTACCTC 
AACTTGAACC TCCTCTTCAT GAATACCGAC ACCGCACCAG CATCTCCCTG CCCCTCCATA 
TCCTCCCAGA ACTCGAGTTC CTGCTCCAGT TTCCAGGACC AAAAGATTGC CAGCATGTTC 
GATCTGACCC CGGAGTACCG GCAGCAGCAC TTCCTTACAG GGCTGCTCTT CACGGAGCTG 
GCTGTTGCCC TGGATGCTGA GGGGGATGGA ATTAGCAGAG TACAGAGAAA AGCCGTGAGT 
GCCATCCACA GCCTTCTGTG TTCTCACGAC CTGGATCCAC GGTGTCGCAA ACCGGAAGTG 
AAAGTCAAAA TCGCCGCCCT TTACCTGCCG TTGGTCGGCA TCATTCTGGA CGCTCTGCCA 
CAGCTCTATG ACTTTACAGA TGCTCGCAGT GGAAGGAGTC GTGCCAGTGG CTCGTATGAA 
GAACAAGATG TGGCCAACGG AATCAACCAG AATGTGGCCC TGGCCATAGC GGGGAATCAC 
TTTAATTTGA AGACCAGTGG AGCAATGCTG TCTTCCTTGC CCTATAAGCA GTACAACATG 
CTGAATGCAG ACACCACCCG CCACCTCATG ATTTGCTTCC TGTGGATCAT GAAAAATGCT 
GATCAGAGCC TCATCAGGAA GTGGATCGCT GACCTGCCTT CCATGCAGCT CAATAGGATT 
CTGGACCTGC TGTTCATCTG TGTCTCCTGC TTTGAATACA AGGGAAAGCA GAGTTCTGAC 
AAAGTCAGTA ACCAGGTCCT GCAGAAGTCA AGAGACGTCA AGGCCAAGCT GGAAGAGGCC 
CTGCTCCGTG GGGAAGGAGC CCGTGGGGAG ATGATGCGCC GTCGCATTCC AGGGACTGAC 
CGGTTTCCAG GCATAAATGA AAATCTGAGA TGGAGGAAAG AGCAGACACA GTGGCGGCAG 
GCTAATGAGA AGCTGGACAA AACAAAGGCA GAGTTAGATC AAGAAGCCTT GATCAGTGGC 
AACCTGGCTA CAGAAGCTAA TTTGATCATC CTGGATATGC AGGAGAACAT CATCCAGGCA 
AGCTCCGCCC TGGACTGTAA AGACAGCCTG CTGGGAGGTG TCCTCCGGGT CCTGGTGAAT 
TCTCTGAGCT GTGACCAGAG CACCACCTAC CTGACTCACT GTTTCGCAAC CCTCCGAGCC 
CTCATCGCCA AGTTTGGAGA CCTGCTGTTC GAGGAGGAGA TGGAGCAGTG TGCTGACCTG 
TGTCAGCGGG TGCTACATCA CTGCAGCAGC AGCATGGACG TCACACGGAG CCAAGCCTGC 
GCCACCCTCT ACCTCCTCAT GCGGTTCAGC TTCGGAGCCA CCAGTAACTT TGCAAGGGTA 
AAGATGCAAG TGACCATGGC ACTGGCATCC CTGGTAGGCA AAGCACCAGA CTTCAACGAA 
GAGCACCTGA GAAGGTCCTT AAGGACAATT TTGGCCTATT CAGAAGAGGA CACGGCCATG 
CAGACAACTC CTTTTCCCAT GCAGGTGGAG GAACTTCTCT GCAATCTGAA CAGCATTCTG 
TACGACACAG TGAAGATGAG GGAATTCCAG GAAGACCCTG AGATGCTTAT GGACCTCATG 
TACAGAATTG CCAAGAGCTA CCAGGCATCG CCTGACCTGC GGCTGACTTG GCTCCAGAAC 
ATGGCAGAGA AACACACTAA GAAGAAGTGC TTCACAGAGG CCGCCATGTG CCTGGTGCAT 
GCAGCCGCCC TGGTGGCCGA GTACCTGAGC ATGCTGGAGG ACCACAGCTA CCTGCCGGTG 
GGCAGCGTCA GCTTTCAGAA TATTTCTTCC AATGTGCTTG AGGAGTCTGC AGTCTCTGAT 
GACACCTTGT CACCTGATGA GGACGGCGTA TGCTCTGGTC GGTACTTCAC TGAGAGTGGC 
CTGGTGGGCC TCCTGGAGCA GGCTGCGGAG CTCTTCAGCA CGGGAGGCTT GTACGAGACG 
GTTAATGAAG TCTACAAGCT GGTCATCCCT ATCCTGGAGG CACACAGAGA TTTCCGGAAG 
CTGACCTCCA CTCACGACAA GCTGCAGAAG GCCTTCGATA ACATCATCAA CAAGGACCAT 
AAGAGGATGT TTGGGACCTA CTTCCGAGTT GGTTTCTACG GATCCCGATT TGGGGATTTG 
GATGAGCAGG AGTTCGTGTA CAAGGAACCC GCAATCACGA AGCTCCCGGA GATCTCACAT 
AGACTAGAGG GATTTTATGG CCAGTGTTTC GGTGCAGAGT TTGTGGAAGT GATAAAAGAC 
TCTACTCCAG TGGACAAAAC CAAGTTGGAT CCTAACAAGG CCTACATTCA GATCACTTTT 
GTGGAGCCTT ACTTTGATGA ATATGAGATG AAAGACCGGG TGACCTACTT CGAGAAGAAT 
TTCAACCTCC GGAGGTTCAT GTACACCACC CCCTTCACCC TGGAGGGGAG ACCCCGGGGC 
GAGCTTCATG AGCAACACCG CAGAAACACC GTGCTCACCA CCATGCACGC CTTCCCCTAC 
ATCAAGACCA GGATCCGAGT CAGCCAGAAA GAGGAGTTCG TTTTGACTCC GATTGAGGTT 
GCCATTGAAG ATATGAAGAA GAAGACCCTG CAGTTAGCCG TGGCCACTCA CCAGGAGCCC 
CCTGATGCAA AGATGCTGCA AATGGTACTG CAGGGCTCTG TAGGAGCCAC TGTAAATCAG 
GGACCACTGG AGGTGGCCCA AGTGTTCTTG GCTGAAATTC CAGCTGACCC AAAGCTCTAC 
CGACATCACA ACAAGCTGAG GTTGTGCTTC AAGGAGTTCA TAATGCGATG CGGAGAGGCC 
GTGGAGAAGA ACAGGCGACT CATCACCGCA GAGCAGCGGG AGTACCAGCA GGAGCTGAAG 
AAGAACTACA ACAAGCTGAG AGACAGCCTC AGGCCCATGA TTGAGCGGAA AATCCCAGAG 
CTCTACAAGC CCATATTCAG AGTTGACAGT CAGAAGAGGG ACTCTTTCCA CAGATCTAGT 
TTCAGGAAAT GTGAAACCCA GTTGTCACAG GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α
Immunity51(1)64-76.e7(2019 07)
Liu D,Yin X,Olyha SJ,Nascimento MSL,Chen P,White T,Gowthaman U,Zhang T,Gertie JA,Zhang B,Xu L,Yurieva M,Devine L,Williams A,Eisenbarth SC

Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery.
European journal of immunology49(5)770-781(2019 May)
Wilson AS,Law HD,Knobbe-Thomsen CB,Kearney CJ,Oliaro J,Binsfeld C,Burgio G,Starrs L,Brenner D,Randall KL,Brüstle A

WASP-mediated regulation of anti-inflammatory macrophages is IL-10 dependent and is critical for intestinal homeostasis.
Nature communications9(1)1779(2018 05)
Biswas A,Shouval DS,Griffith A,Goettel JA,Field M,Kang YH,Konnikova L,Janssen E,Redhu NS,Thrasher AJ,Chatila T,Kuchroo VK,Geha RS,Notarangelo LD,Pai SY,Horwitz BH,Snapper SB

Migratory CD11b
Science immunology2(18)(2017 12)
Krishnaswamy JK,Gowthaman U,Zhang B,Mattsson J,Szeponik L,Liu D,Wu R,White T,Calabro S,Xu L,Collet MA,Yurieva M,Alsén S,Fogelstrand P,Walter A,Heath WR,Mueller SN,Yrlid U,Williams A,Eisenbarth SC

An Anti-Inflammatory Role for NLRP10 in Murine Cutaneous Leishmaniasis.
Journal of immunology (Baltimore, Md. : 1950)199(8)2823-2833(2017 10)
Clay GM,Valadares DG,Graff JW,Ulland TK,Davis RE,Scorza BM,Zhanbolat BS,Chen Y,Sutterwala FS,Wilson ME

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Dock8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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