Adgrf5 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006524129.2	XP_006524192.1	adhesion G protein-coupled receptor F5 isoform X4
XM_006524125.3	XP_006524188.1	adhesion G protein-coupled receptor F5 isoform X1
XM_006524128.4	XP_006524191.1	adhesion G protein-coupled receptor F5 isoform X3
XM_006524126.3	XP_006524189.1	adhesion G protein-coupled receptor F5 isoform X2
XM_006524129.3	XP_006524192.1	adhesion G protein-coupled receptor F5 isoform X4
NM_001081178.1	NP_001074647.1	adhesion G protein-coupled receptor F5 isoform 1 precursor
XM_006524125.4	XP_006524188.1	adhesion G protein-coupled receptor F5 isoform X1
XM_006524128.3	XP_006524191.1	adhesion G protein-coupled receptor F5 isoform X3
XM_017317437.1	XP_017172926.1	adhesion G protein-coupled receptor F5 isoform X4
NM_001357332.1	NP_001344261.1	adhesion G protein-coupled receptor F5 isoform 2 precursor
XM_006524124.3	XP_006524187.1	adhesion G protein-coupled receptor F5 isoform X1
XM_006524124.4	XP_006524187.1	adhesion G protein-coupled receptor F5 isoform X1
XM_006524126.4	XP_006524189.1	adhesion G protein-coupled receptor F5 isoform X2
XM_006524127.3	XP_006524190.1	adhesion G protein-coupled receptor F5 isoform X3

The following Adgrf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgrf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13458	NM_001081178.1 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53486	NM_001357332.1 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53483	XM_006524124.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53483	XM_006524125.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53484	XM_006524126.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53485	XM_006524127.3 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53485	XM_006524128.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53486	XM_006524129.3 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53486	XM_017317437.1 Latest version!	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMu53486	XM_006524129.2	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53483	XM_006524125.3	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53485	XM_006524128.3	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53483	XM_006524124.3	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53484	XM_006524126.3	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
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Reference Sequences (Refseq)

Adgrf5 ( NM_001081178.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074647.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13458
Clone ID Related Accession (Same CDS sequence)	NM_001081178.1
Accession Version	NM_001081178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC169504.1, CAAA01049588.1 and CAAA01202959.1. On Feb 6, 2007 this sequence version replaced XM_283438.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074647.1
CDS	267..4313
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	NM_001081178.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081178.1

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( NM_001357332.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344261.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu53486
Clone ID Related Accession (Same CDS sequence)	XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
Accession Version	NM_001357332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562256000000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025700.16, AC169504.1, CT010585.12 and AW457181.1. On Oct 25, 2017 this sequence version replaced XM_017317437.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.213531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344261.1
CDS	191..4291
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	NM_001357332.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357332.1

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524125.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524188.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

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CloneID	OMu53483
Clone ID Related Accession (Same CDS sequence)	XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
Accession Version	XM_006524125.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524188.1
CDS	295..4515
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524125.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524125.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524125.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524188.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

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CloneID	OMu53483
Clone ID Related Accession (Same CDS sequence)	XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
Accession Version	XM_006524125.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524188.1
CDS	293..4513
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524125.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524125.4

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524124.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524187.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53483
Clone ID Related Accession (Same CDS sequence)	XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
Accession Version	XM_006524124.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524124.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524187.1
CDS	316..4536
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524124.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524124.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524124.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524187.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53483
Clone ID Related Accession (Same CDS sequence)	XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
Accession Version	XM_006524124.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524124.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524187.1
CDS	650..4870
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524124.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524124.4

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524126.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524189.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

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CloneID	OMu53484
Clone ID Related Accession (Same CDS sequence)	XM_006524126.3 , XM_006524126.4
Accession Version	XM_006524126.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA 
CCCCGATGGA ACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524189.1
CDS	321..4538
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTAAPKAPQGNDGNLVSSGSPSPRWNI

Target ORF information:

RefSeq Version	XM_006524126.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524126.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA 
CCCCGATGGA ACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524126.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524189.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

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CloneID	OMu53484
Clone ID Related Accession (Same CDS sequence)	XM_006524126.3 , XM_006524126.4
Accession Version	XM_006524126.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524126.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA 
CCCCGATGGA ACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524189.1
CDS	650..4867
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTAAPKAPQGNDGNLVSSGSPSPRWNI

Target ORF information:

RefSeq Version	XM_006524126.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524126.4

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA 
AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT 
AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA 
CCCCGATGGA ACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524128.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524191.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

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CloneID	OMu53485
Clone ID Related Accession (Same CDS sequence)	XM_006524128.4 , XM_006524128.3 , XM_006524127.3
Accession Version	XM_006524128.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524191.1
CDS	292..4458
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524128.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524128.4

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524128.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524191.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

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CloneID	OMu53485
Clone ID Related Accession (Same CDS sequence)	XM_006524128.4 , XM_006524128.3 , XM_006524127.3
Accession Version	XM_006524128.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524191.1
CDS	294..4460
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524128.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524128.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524127.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524190.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.

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CloneID	OMu53485
Clone ID Related Accession (Same CDS sequence)	XM_006524128.4 , XM_006524128.3 , XM_006524127.3
Accession Version	XM_006524127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524190.1
CDS	548..4714
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVGDNASYKGEVPEVLRHVMEVSTVQSTTGNSLETSKPQEQPANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524127.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524127.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG 
CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG 
GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC 
ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA 
GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT 
GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC 
CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA 
TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC 
TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC 
ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG 
TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC 
ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG 
CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT 
GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT 
GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT 
AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA 
ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC 
ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG 
GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA 
ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT 
CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT 
CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT 
ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG 
ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA 
TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC 
TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT 
ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA 
GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC 
GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC 
TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG 
GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG 
GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA 
ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC 
ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG 
AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC 
ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC 
CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA 
GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT 
TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC 
TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT 
TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT 
GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC 
AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC 
CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC 
GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG 
AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC 
TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC 
TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC 
AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT 
CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT 
CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG 
AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC 
GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG 
AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC 
AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA 
GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG 
GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG 
CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG 
TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT 
AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC 
TCTAGTGCTT ACTCACTGCT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524129.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524192.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu53486
Clone ID Related Accession (Same CDS sequence)	XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
Accession Version	XM_006524129.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524192.1
CDS	315..4415
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524129.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524129.2

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006524129.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524192.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu53486
Clone ID Related Accession (Same CDS sequence)	XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
Accession Version	XM_006524129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524192.1
CDS	650..4750
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006524129.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524129.3

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_017317437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172926.1 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X7, mRNA.

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CloneID	OMu53486
Clone ID Related Accession (Same CDS sequence)	XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
Accession Version	XM_017317437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172926.1
CDS	191..4291
Translation	MRSPRTFTFYFLLLVICSSEAALSTPTEPIVQPSVSKAKPAVISSAQVFDFPILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDAGMYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNHPISLNCCSENIANWSSIEWKQEGKISILGNPESDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSAYGARGSKNIAVTFTSVANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAEREVIGKQACYTYSLPANLPRCPKDIAVFCHFTNAANSSVRSPSMKLKLIPRENVTCQDPIIGIGDPGKVIQKLCQFSGVYGSPGQAIGGTVTYKCVGTQWKEESRACISAPINGLLQVAKALIKSPTQDQKLPTYLRDLSVSAGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSALNSWEKLLQQQSNQSSQFLHSVERFSQALQLGDSTPPFLAHPNVQMKSMVIKRGHPQIYQQQFIFKDSDLWGDVAIDECQLGNLQPDSSIVTVAFPTLKAILAQDVQRKTSSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCSVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIAFCLLIADIWFIVAGAIHDGRYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSIGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSIGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSLENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_017317437.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317437.1

ORF Insert Sequence:

ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT 
GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC 
GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA 
TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT 
CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA 
ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC 
CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG 
CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC 
CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC 
CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC 
CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG 
GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT 
CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC 
TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT 
GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG 
TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT 
GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT 
GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA 
TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC 
CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG 
AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC 
AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA 
GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT 
GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG 
GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG 
AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT 
GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC 
TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG 
CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT 
TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC 
CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC 
ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC 
TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA 
AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT 
CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT 
GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT 
CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG 
AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC 
CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT 
CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA 
GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT 
CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT 
CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC 
CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT 
GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA 
ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA 
ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG 
AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC 
AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG 
GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC 
TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG 
GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT 
GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC 
CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA 
CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC 
TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC 
GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC 
ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC 
GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG 
ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC 
TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT 
ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC 
CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC 
TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG 
TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA 
CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA 
GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT 
GCTTACTCAC TGCTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells.
Respiratory research20(1)11(2019 Jan)
Kubo F,Ariestanti DM,Oki S,Fukuzawa T,Demizu R,Sato T,Sabirin RM,Hirose S,Nakamura N

Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4).
PloS one12(8)e0183166(2017)
Lu S,Liu S,Wietelmann A,Kojonazarov B,Atzberger A,Tang C,Schermuly RT,Gröne HJ,Offermanns S

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Targeted Disruption of Ig-Hepta/Gpr116 Causes Emphysema-like Symptoms That Are Associated with Alveolar Macrophage Activation.
The Journal of biological chemistry290(17)11032-40(2015 Apr)
Ariestanti DM,Ando H,Hirose S,Nakamura N

Gpr116 Receptor Regulates Distinctive Functions in Pneumocytes and Vascular Endothelium.
PloS one10(9)e0137949(2015)
Niaudet C,Hofmann JJ,Mäe MA,Jung B,Gaengel K,Vanlandewijck M,Ekvärn E,Salvado MD,Mehlem A,Al Sayegh S,He L,Lebouvier T,Castro-Freire M,Katayama K,Hultenby K,Moessinger C,Tannenberg P,Cunha S,Pietras K,Laviña B,Hong J,Berg T,Betsholtz C

Gene Symbol	Adgrf5
Entrez Gene ID	224792
Full Name	adhesion G protein-coupled receptor F5
Synonyms	8430401C09Rik,9330185D23,AI528491,Gpr116,mKIAA0758
General protein information	Preferred Names adhesion G protein-coupled receptor F5 Names adhesion G protein-coupled receptor F5 G protein coupled receptor Gpr116 G protein-coupled receptor 116 probable G-protein coupled receptor 116
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B3

Pan troglodytes (chimpanzee)	GPR116	XP_001140861.2
Gallus gallus (chicken)	GPR116	XP_004935990.1
Xenopus tropicalis (tropical clawed frog)	gpr116	NP_001107298.1
Mus musculus (house mouse)	Gpr116	NP_001074647.1
Homo sapiens (human)	GPR116	NP_001091988.1
Macaca mulatta (Rhesus monkey)	GPR116	XP_001103527.2
Canis lupus familiaris (dog)	GPR116	XP_005627499.1
Bos taurus (cattle)	GPR116	NP_001180172.1
Rattus norvegicus (Norway rat)	Gpr116	NP_620810.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Adgrf5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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