TSC2 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC2
Entrez Gene ID	100670924
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Loxodonta africana(African savanna elephant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023557525.1	XP_023413293.1	tuberin isoform X5
XM_010597416.2	XP_010595718.1	tuberin isoform X4
XM_010597417.2	XP_010595719.1	tuberin isoform X1
XM_010597416.1	XP_010595718.1	tuberin isoform X1
XM_023557526.1	XP_023413294.1	tuberin isoform X6
XM_023557524.1	XP_023413292.1	tuberin isoform X3
XM_010597417.1	XP_010595719.1	tuberin isoform X2
XM_023557523.1	XP_023413291.1	tuberin isoform X2

Pathways from BioSystems
Gene Ontology

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLo105847	XM_023557525.1 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OLo105845	XM_023557523.1 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLo105846	XM_023557524.1 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLo105848	XM_023557526.1 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLo49915	XM_010597416.2 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLo49916	XM_010597417.2 Latest version!	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OLo49915	XM_010597416.1	Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLo49916	XM_010597417.1	Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC2 ( XM_023557525.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023413293.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OLo105847
Clone ID Related Accession (Same CDS sequence)	XM_023557525.1
Accession Version	XM_023557525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACTCA TCAAGAAGTA CAGGAAGGAG CTGCAGGCTG TGACGTGGGA CATCCTGCTG 
AGCATCATCG AACGTCTGCT CCAGCAGCTC CTGACCCTCG AGAGTCAGGA GCTCAGAGCC 
ATCGTGCATG ACCTGCTGAC GACGGTGGAG GAGCTGTGTG ACCAGAATGA GTTCCACGGC 
TCACAGGAGA GATACTTTGA GCTCATTGAG CGATGCGCCG ACCAGAGACC TGAGTCCTCC 
CTCTTGAACC TGATATCCTA CAGGGCACAG TCCATCCACC CAGCCAAGGA TGACTGGATC 
CACAACCTGC AGCAGTTAAT GGAGAGGTTC TTCAGGAACG AGTCCCGCAG TGCTGTGCGC 
ATCAAGGTGC TGGACATCCT GTCCTTTGTG CTGCTTATCA ACCGGCAGTT CTATGAGGAG 
GAGCTGATCA ACTCAGTGGT CATCTCCCAA CTCTCCCACA TTCCAGAAGA CAGAGACCCA 
CAGGTCCGCA AGCTGGCCAC CCAGTTGCTG GTGGACCTGG CGGAAGGCTG CCACACCCAC 
CACTTCAATA ATCTTCTGGA CATCATTGAG AAAGTGATTG CTCGCTCCCT GTTCCCTCCT 
CCTGAGCTAG AAGAAAGAGA TGTGCCTGCG TACTCGGCCT CCTTGGAGGA TGTGAAGACT 
GCGGTCCTGG GGCTCCTGGT CATCCTTCAG ACCAAGCTCT ACACTTTGCC TGCCAGCCAT 
GCAGCACGGG TATACGAGAT GCTTGTCCAC CACATCCAGC TGCACTACAA GCACATGTAC 
ACCTTGCCCA TTGCCAGCAG CATCCGGCTT CAGGCCTTTG ACTTCCTGCT GCTGCTCCGG 
GCAGATGCAC TACACCGCCT GGGCCTGCCT AACAAGGATG GTGCTGTACG GTTCAGCTCC 
TACTGCCTCT GTGACCACAT GGAGCCAGAG CACGGCTTGG AGAAGAAGGC CAGTGGCCCC 
GTCTCGCCTC CCACGGGGCC TACCGTCCCA GCACCTGCAG GCCCCACTGT GCGGCTTGGC 
TATCTGCCCT ACTCCTTGCT CTTCCAAGTC CTGCTGCAGT GTCTGAAGCA GGAGACGGAC 
TGGAAGGTGC TGAAGCTGGT ACTCAGCAAG CTGCCGGAGT CCCTGCGCTA CAAGGTCCTC 
ATCTTCACAT CCCCCTGCAG CGTCGACCAG CTGTCCTCTG CCCTCTGCTC CATGCTTTCA 
GGTCCCAAGA CCCTGGAACG ACTCCGAGGC ACACCAGAGG GCTTCTCTAG AACCGACTTA 
CACTTGGCAG TGGTTCCAGT GTTGACAGCA CTGATCTCTT ACCATAACTA CCTGGACAAA 
ACCAAACAGC GCGAGATGGT GTACTGCTTG GAGCAAGGCC TCATCTACCG CTGTGCCAGC 
CAATGTGTGG TGGCCCTGGC TGTGTGCAGT GTGGAGATGC CTGACGTCAT CATCAAGGCA 
CTGCCCGTAC TGATTGTAAA GCTCACGCAC ATCTCGGCCA CAGCCAGCAT GGCCATCCCG 
CTGCTCGAGT TCCTGTCGAC TCTGGCCCGG CTGCCGCATC TCTACAGGAA CTTTGCTGCT 
GAGCAGTACG CCAGCGTTTT CGCCATTTCA CTGCCCTACA CCAACCCATC CAAATTCAAT 
CAGTATATTG TGTGCCTGGC CCACCACGTG ATAGCCATGT GGTTCATTCG GTGCCGCCTG 
CCCTTCCGGA AAGATTTTGT TCCGTATATC ACTAAGGGCC TTCGCTCCAA TGTCCTCCTG 
TCTTTTGATG ACACCCCCGA GAAGGACAGC TTCAGGGCAC GGAGCACCAG TCTCAACGAG 
AGGCCCAAAA GTAGGATCCA GACCTCCTTC ACCAGTGCCA GCTTGGGTTC TGCAGACGAA 
AACTCGATGG CCCAGGCAGA CGACAACCTG AAAAATCTCC ACCTGGAGCT CACCGAGACG 
TGCCTGGACA TGATGGCCAG ATACGTGTTC TCCAACTTCA CAGCTGTCCC CAAGAGGTCT 
CCCGTGGGAG AATTCCTCCT GGCTGGAGGC AGGACCAAAA CCTGGCTGGT GGGGAACAAG 
CTGGTTACCG TAACGACGAG TGTGGGAACT GGCACACGGT CGCTGCTGGG CCTAGACCCA 
GGCGAGCTGC AGGGTGGCTC AGAGCTTAGC TCCAGCCCTG GTGTACACGT GAGGCAGACG 
AAAGAGGCGC CAGCCAAGCT GGAGTCCCAG GCTGGGGTAC AGGTGTGCCG CAGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGTGGCCAT GGCCTGCGCG TGGGAGCCCT GGACACTCTA 
GCCTCCTACT TCCCTGGAGG CCCTATTTGT CCAGGGCCGC AGACCATGCC GGTCGTGAAG 
CCTCAGAAAG CCTCAGCGAG CACCCAGATG CCAGTGCCAA AGGAAAAGAC AAACCTGGCT 
GCCTACGTGC CCCTCCTGAC CCAGGGCTGG GCTGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGTT GGTTGATGAG CCTGGAGAAT CCACTAAGCC CTTTCTCTTC GGACATCAAC 
AATATGCCAC TGCAGGAGTT GTCCAATGCT CTCATGGCCG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CCCTGTACAA GTCATTGTCG GTGCCAGTGG CCAGCGCCTC CAAGCCCTCC 
CTCCTGCCCC GCTCCAACAC AGACTCCCCG GGGGTTCTGG AGGAAGGATG TGCAGGTGAG 
ATGAACTTGT CCACTGAGCT TCCTGAGCTT GAGGACTTCA AGGCAGCCTC AGGTTCAGAC 
AGGCGCTCCC AGCGTACCGA TGCTTACAGT AGGTCATCTT CGATCTCCAG CCAAGAGGAG 
AAGCAGCTGT GTGCAGAGGA GCTGGCTGCC AGGGGAATCC CCATTGAGCA GGTGGTGTCC 
TCCAGGGGTG GCCAGCCCTG TGTGGACCTC TCCTTTCAGC CCTCGCAGCC TCTAAGCAAA 
TCCAGCTCCT CACCCGAACT GCAGACCTTG CAGGACATCT TTGGGGACGC TGGGGACAAG 
GCCGATGGAG GCTGGCTGAG CCCTGAGGCC AAGGCCCGGT GGCAGTCGGG GACCCTGGAT 
GGGGAGTGTG CTGCCTGGCT GGCTCCAGGT GAGGAGGGCC GGGCCCATGC CCCAGCCCAG 
CACGAGGGCT CACTGCCCTG CAGCTCCCCA CGCTCCCCAC ACGGCCTGCG GCCCCGAGGC 
TACACCATTT CCGACCCAGT CCAGTTGCGC AGGGGCAAGA CAGTGGAGAG AGACACCTCC 
AACAGCCGAG CTGCTGCCTC CAACGCGGAG AAGGTGCCAG GCATCAACCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC GCCCTTCTTT GGGGACGAGT CCAACAAGCC AATTCTGTTA 
CCCAACGAGT CCTTCGAGCG CTCGGTGCAG CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATCG CTGTTTTGTA CGTGGGAGAG GGCCAGAGCA ACAGTGAGCT CGCCATCCTC 
TCCAACGAGC ACGGCTCCTA CAGGTATACA GAATTCCTGA CGGGCCTGGG CAAGCTCATT 
GAGCTCAAGG ACTGCCAGCC TGACAAGGTG TACCTGGGTG GCTTGGATGT GGGCGGCGAG 
GACGGCCAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCAGTCTT TCACATCGCC 
ACCCTGATGC CCACCAAGGA TGGGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATTGT CTACAACGAC TCTGGCGAGG ACTTCAGGCT GGGCACCATC 
AAGGGCCAGT TCAACTTCGT GCATGTGATT ATCACTCCTC TGGACTATGA GTGCAACCTG 
GTCTCACTCC AGTGCCGGAA AGACATGGAG GGCCTGGTGG ACACCAGTAT GGCCAAGATC 
GTGTCTGACT GTAACCTGCC CTTCGTGGCC CGCCAGATGG CCTTGCATGC CAACATGGCC 
TCCCAGGTCC ACCACAGCCG CTCTAACCCC ACTGACATCT ACCCTTCCAA GTGGATTGCC 
CGGCTCCGCC ACATCAAGCG GCTACGTCAC CGGATCCGGG AGGAAGCCAA TTACCTGAAC 
CCTAGCCTGC CTCTGGTGCA GATGCATCCT CCGGCCAACA CCAAGGCCCC TGCAGAGGCC 
ACACCCACCT ATGAAACCGG CCAGAGAAAG CGCCTCATCT CCTCAGTAGA CGACTTCACA 
GAATTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023413293.1
CDS	107..4318
Translation	MRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_023557525.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023557525.1

ORF Insert Sequence:

ATGAGACTCA TCAAGAAGTA CAGGAAGGAG CTGCAGGCTG TGACGTGGGA CATCCTGCTG 
AGCATCATCG AACGTCTGCT CCAGCAGCTC CTGACCCTCG AGAGTCAGGA GCTCAGAGCC 
ATCGTGCATG ACCTGCTGAC GACGGTGGAG GAGCTGTGTG ACCAGAATGA GTTCCACGGC 
TCACAGGAGA GATACTTTGA GCTCATTGAG CGATGCGCCG ACCAGAGACC TGAGTCCTCC 
CTCTTGAACC TGATATCCTA CAGGGCACAG TCCATCCACC CAGCCAAGGA TGACTGGATC 
CACAACCTGC AGCAGTTAAT GGAGAGGTTC TTCAGGAACG AGTCCCGCAG TGCTGTGCGC 
ATCAAGGTGC TGGACATCCT GTCCTTTGTG CTGCTTATCA ACCGGCAGTT CTATGAGGAG 
GAGCTGATCA ACTCAGTGGT CATCTCCCAA CTCTCCCACA TTCCAGAAGA CAGAGACCCA 
CAGGTCCGCA AGCTGGCCAC CCAGTTGCTG GTGGACCTGG CGGAAGGCTG CCACACCCAC 
CACTTCAATA ATCTTCTGGA CATCATTGAG AAAGTGATTG CTCGCTCCCT GTTCCCTCCT 
CCTGAGCTAG AAGAAAGAGA TGTGCCTGCG TACTCGGCCT CCTTGGAGGA TGTGAAGACT 
GCGGTCCTGG GGCTCCTGGT CATCCTTCAG ACCAAGCTCT ACACTTTGCC TGCCAGCCAT 
GCAGCACGGG TATACGAGAT GCTTGTCCAC CACATCCAGC TGCACTACAA GCACATGTAC 
ACCTTGCCCA TTGCCAGCAG CATCCGGCTT CAGGCCTTTG ACTTCCTGCT GCTGCTCCGG 
GCAGATGCAC TACACCGCCT GGGCCTGCCT AACAAGGATG GTGCTGTACG GTTCAGCTCC 
TACTGCCTCT GTGACCACAT GGAGCCAGAG CACGGCTTGG AGAAGAAGGC CAGTGGCCCC 
GTCTCGCCTC CCACGGGGCC TACCGTCCCA GCACCTGCAG GCCCCACTGT GCGGCTTGGC 
TATCTGCCCT ACTCCTTGCT CTTCCAAGTC CTGCTGCAGT GTCTGAAGCA GGAGACGGAC 
TGGAAGGTGC TGAAGCTGGT ACTCAGCAAG CTGCCGGAGT CCCTGCGCTA CAAGGTCCTC 
ATCTTCACAT CCCCCTGCAG CGTCGACCAG CTGTCCTCTG CCCTCTGCTC CATGCTTTCA 
GGTCCCAAGA CCCTGGAACG ACTCCGAGGC ACACCAGAGG GCTTCTCTAG AACCGACTTA 
CACTTGGCAG TGGTTCCAGT GTTGACAGCA CTGATCTCTT ACCATAACTA CCTGGACAAA 
ACCAAACAGC GCGAGATGGT GTACTGCTTG GAGCAAGGCC TCATCTACCG CTGTGCCAGC 
CAATGTGTGG TGGCCCTGGC TGTGTGCAGT GTGGAGATGC CTGACGTCAT CATCAAGGCA 
CTGCCCGTAC TGATTGTAAA GCTCACGCAC ATCTCGGCCA CAGCCAGCAT GGCCATCCCG 
CTGCTCGAGT TCCTGTCGAC TCTGGCCCGG CTGCCGCATC TCTACAGGAA CTTTGCTGCT 
GAGCAGTACG CCAGCGTTTT CGCCATTTCA CTGCCCTACA CCAACCCATC CAAATTCAAT 
CAGTATATTG TGTGCCTGGC CCACCACGTG ATAGCCATGT GGTTCATTCG GTGCCGCCTG 
CCCTTCCGGA AAGATTTTGT TCCGTATATC ACTAAGGGCC TTCGCTCCAA TGTCCTCCTG 
TCTTTTGATG ACACCCCCGA GAAGGACAGC TTCAGGGCAC GGAGCACCAG TCTCAACGAG 
AGGCCCAAAA GTAGGATCCA GACCTCCTTC ACCAGTGCCA GCTTGGGTTC TGCAGACGAA 
AACTCGATGG CCCAGGCAGA CGACAACCTG AAAAATCTCC ACCTGGAGCT CACCGAGACG 
TGCCTGGACA TGATGGCCAG ATACGTGTTC TCCAACTTCA CAGCTGTCCC CAAGAGGTCT 
CCCGTGGGAG AATTCCTCCT GGCTGGAGGC AGGACCAAAA CCTGGCTGGT GGGGAACAAG 
CTGGTTACCG TAACGACGAG TGTGGGAACT GGCACACGGT CGCTGCTGGG CCTAGACCCA 
GGCGAGCTGC AGGGTGGCTC AGAGCTTAGC TCCAGCCCTG GTGTACACGT GAGGCAGACG 
AAAGAGGCGC CAGCCAAGCT GGAGTCCCAG GCTGGGGTAC AGGTGTGCCG CAGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGTGGCCAT GGCCTGCGCG TGGGAGCCCT GGACACTCTA 
GCCTCCTACT TCCCTGGAGG CCCTATTTGT CCAGGGCCGC AGACCATGCC GGTCGTGAAG 
CCTCAGAAAG CCTCAGCGAG CACCCAGATG CCAGTGCCAA AGGAAAAGAC AAACCTGGCT 
GCCTACGTGC CCCTCCTGAC CCAGGGCTGG GCTGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGTT GGTTGATGAG CCTGGAGAAT CCACTAAGCC CTTTCTCTTC GGACATCAAC 
AATATGCCAC TGCAGGAGTT GTCCAATGCT CTCATGGCCG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CCCTGTACAA GTCATTGTCG GTGCCAGTGG CCAGCGCCTC CAAGCCCTCC 
CTCCTGCCCC GCTCCAACAC AGACTCCCCG GGGGTTCTGG AGGAAGGATG TGCAGGTGAG 
ATGAACTTGT CCACTGAGCT TCCTGAGCTT GAGGACTTCA AGGCAGCCTC AGGTTCAGAC 
AGGCGCTCCC AGCGTACCGA TGCTTACAGT AGGTCATCTT CGATCTCCAG CCAAGAGGAG 
AAGCAGCTGT GTGCAGAGGA GCTGGCTGCC AGGGGAATCC CCATTGAGCA GGTGGTGTCC 
TCCAGGGGTG GCCAGCCCTG TGTGGACCTC TCCTTTCAGC CCTCGCAGCC TCTAAGCAAA 
TCCAGCTCCT CACCCGAACT GCAGACCTTG CAGGACATCT TTGGGGACGC TGGGGACAAG 
GCCGATGGAG GCTGGCTGAG CCCTGAGGCC AAGGCCCGGT GGCAGTCGGG GACCCTGGAT 
GGGGAGTGTG CTGCCTGGCT GGCTCCAGGT GAGGAGGGCC GGGCCCATGC CCCAGCCCAG 
CACGAGGGCT CACTGCCCTG CAGCTCCCCA CGCTCCCCAC ACGGCCTGCG GCCCCGAGGC 
TACACCATTT CCGACCCAGT CCAGTTGCGC AGGGGCAAGA CAGTGGAGAG AGACACCTCC 
AACAGCCGAG CTGCTGCCTC CAACGCGGAG AAGGTGCCAG GCATCAACCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC GCCCTTCTTT GGGGACGAGT CCAACAAGCC AATTCTGTTA 
CCCAACGAGT CCTTCGAGCG CTCGGTGCAG CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATCG CTGTTTTGTA CGTGGGAGAG GGCCAGAGCA ACAGTGAGCT CGCCATCCTC 
TCCAACGAGC ACGGCTCCTA CAGGTATACA GAATTCCTGA CGGGCCTGGG CAAGCTCATT 
GAGCTCAAGG ACTGCCAGCC TGACAAGGTG TACCTGGGTG GCTTGGATGT GGGCGGCGAG 
GACGGCCAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCAGTCTT TCACATCGCC 
ACCCTGATGC CCACCAAGGA TGGGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATTGT CTACAACGAC TCTGGCGAGG ACTTCAGGCT GGGCACCATC 
AAGGGCCAGT TCAACTTCGT GCATGTGATT ATCACTCCTC TGGACTATGA GTGCAACCTG 
GTCTCACTCC AGTGCCGGAA AGACATGGAG GGCCTGGTGG ACACCAGTAT GGCCAAGATC 
GTGTCTGACT GTAACCTGCC CTTCGTGGCC CGCCAGATGG CCTTGCATGC CAACATGGCC 
TCCCAGGTCC ACCACAGCCG CTCTAACCCC ACTGACATCT ACCCTTCCAA GTGGATTGCC 
CGGCTCCGCC ACATCAAGCG GCTACGTCAC CGGATCCGGG AGGAAGCCAA TTACCTGAAC 
CCTAGCCTGC CTCTGGTGCA GATGCATCCT CCGGCCAACA CCAAGGCCCC TGCAGAGGCC 
ACACCCACCT ATGAAACCGG CCAGAGAAAG CGCCTCATCT CCTCAGTAGA CGACTTCACA 
GAATTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_023557523.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023413291.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLo105845
Clone ID Related Accession (Same CDS sequence)	XM_023557523.1
Accession Version	XM_023557523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023413291.1
CDS	142..5376
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_023557523.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023557523.1

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_023557524.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023413292.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OLo105846
Clone ID Related Accession (Same CDS sequence)	XM_023557524.1
Accession Version	XM_023557524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGACA TGCCGTGGAA GCACTGTGGA AAGCTGTCGC GGACTTGCTG 
CAGCCAGACC GGCCACCAGA GGCACGACAT GCAGTGCTGG CCCTGCTCAA GGCCATCGTG 
CAGGGCCAGG GTGACCGCCT GGGTGTGCTC CGTGCCCTCT TCTTCAAGGT CATAAAGGAT 
TACCCTTCGA ACAAAGACTT GCACGAAAGG CTGGAGGTGT TCAAGGCCCT GACGGACAAT 
GGGAGACACA TAACCTACTT GGAAGAAGAG CTGGCTGAGT TTGTCCTGCA GTGGATGGAT 
GTGGGCTTAT CCTCAGAATT CCTGCTGGTG CTTGTGAACC TGGTCAAGTT CAACAGCTGT 
TACCTTGATG AATACATCGC ATCCATGGTT CACATGATCT GTCTGTTGTG CATCCGGACC 
TCGTCCTCCG TGGACATAGA GGTGTCCCTG CAGGTGCTGG ATGCCGTGGT CTGCTACAAC 
TGCCTGCCTG CCGCGAGCCT GCCCCTATTC ATTGTCACAT TGTGCCGCAC CGTCAACGTC 
AAGGAGCTCT GCGAGCCCTG CTGGAAGCTG ATGCGTAACC TCCTGGGAAC CCACCTGGGC 
CACAGTGCCA TCTACAACAT GTGCCGCATC ATGGAGGACA GAGCCTATAT GGAGGATGCC 
CCGCTGCTGA GAGGAGCTGT GTTCTTTGTC GGCATGGCGC TGTGGGGGGC CCACCGACTC 
TACTCCCTCA AGAACTCCCC TACATCTGTG CTGCCGTCGT TTTATGAGGC CATGACCTGC 
CCCAATGAGG TGGTGTCCTA CGAAATCGTC CTCTCTATCA CCAGACTCAT CAAGAAGTAC 
AGGAAGGAGC TGCAGGCTGT GACGTGGGAC ATCCTGCTGA GCATCATCGA ACGTCTGCTC 
CAGCAGCTCC TGACCCTCGA GAGTCAGGAG CTCAGAGCCA TCGTGCATGA CCTGCTGACG 
ACGGTGGAGG AGCTGTGTGA CCAGAATGAG TTCCACGGCT CACAGGAGAG ATACTTTGAG 
CTCATTGAGC GATGCGCCGA CCAGAGACCT GAGTCCTCCC TCTTGAACCT GATATCCTAC 
AGGGCACAGT CCATCCACCC AGCCAAGGAT GACTGGATCC ACAACCTGCA GCAGTTAATG 
GAGAGGTTCT TCAGGAACGA GTCCCGCAGT GCTGTGCGCA TCAAGGTGCT GGACATCCTG 
TCCTTTGTGC TGCTTATCAA CCGGCAGTTC TATGAGGAGG AGCTGATCAA CTCAGTGGTC 
ATCTCCCAAC TCTCCCACAT TCCAGAAGAC AGAGACCCAC AGGTCCGCAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC GGAAGGCTGC CACACCCACC ACTTCAATAA TCTTCTGGAC 
ATCATTGAGA AAGTGATTGC TCGCTCCCTG TTCCCTCCTC CTGAGCTAGA AGAAAGAGAT 
GTGCCTGCGT ACTCGGCCTC CTTGGAGGAT GTGAAGACTG CGGTCCTGGG GCTCCTGGTC 
ATCCTTCAGA CCAAGCTCTA CACTTTGCCT GCCAGCCATG CAGCACGGGT ATACGAGATG 
CTTGTCCACC ACATCCAGCT GCACTACAAG CACATGTACA CCTTGCCCAT TGCCAGCAGC 
ATCCGGCTTC AGGCCTTTGA CTTCCTGCTG CTGCTCCGGG CAGATGCACT ACACCGCCTG 
GGCCTGCCTA ACAAGGATGG TGCTGTACGG TTCAGCTCCT ACTGCCTCTG TGACCACATG 
GAGCCAGAGC ACGGCTTGGA GAAGAAGGCC AGTGGCCCCG TCTCGCCTCC CACGGGGCCT 
ACCGTCCCAG CACCTGCAGG CCCCACTGTG CGGCTTGGCT ATCTGCCCTA CTCCTTGCTC 
TTCCAAGTCC TGCTGCAGTG TCTGAAGCAG GAGACGGACT GGAAGGTGCT GAAGCTGGTA 
CTCAGCAAGC TGCCGGAGTC CCTGCGCTAC AAGGTCCTCA TCTTCACATC CCCCTGCAGC 
GTCGACCAGC TGTCCTCTGC CCTCTGCTCC ATGCTTTCAG GTCCCAAGAC CCTGGAACGA 
CTCCGAGGCA CACCAGAGGG CTTCTCTAGA ACCGACTTAC ACTTGGCAGT GGTTCCAGTG 
TTGACAGCAC TGATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTG 
TACTGCTTGG AGCAAGGCCT CATCTACCGC TGTGCCAGCC AATGTGTGGT GGCCCTGGCT 
GTGTGCAGTG TGGAGATGCC TGACGTCATC ATCAAGGCAC TGCCCGTACT GATTGTAAAG 
CTCACGCACA TCTCGGCCAC AGCCAGCATG GCCATCCCGC TGCTCGAGTT CCTGTCGACT 
CTGGCCCGGC TGCCGCATCT CTACAGGAAC TTTGCTGCTG AGCAGTACGC CAGCGTTTTC 
GCCATTTCAC TGCCCTACAC CAACCCATCC AAATTCAATC AGTATATTGT GTGCCTGGCC 
CACCACGTGA TAGCCATGTG GTTCATTCGG TGCCGCCTGC CCTTCCGGAA AGATTTTGTT 
CCGTATATCA CTAAGGGCCT TCGCTCCAAT GTCCTCCTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCACG GAGCACCAGT CTCAACGAGA GGCCCAAAAG TAGGATCCAG 
ACCTCCTTCA CCAGTGCCAG CTTGGGTTCT GCAGACGAAA ACTCGATGGC CCAGGCAGAC 
GACAACCTGA AAAATCTCCA CCTGGAGCTC ACCGAGACGT GCCTGGACAT GATGGCCAGA 
TACGTGTTCT CCAACTTCAC AGCTGTCCCC AAGAGGTCTC CCGTGGGAGA ATTCCTCCTG 
GCTGGAGGCA GGACCAAAAC CTGGCTGGTG GGGAACAAGC TGGTTACCGT AACGACGAGT 
GTGGGAACTG GCACACGGTC GCTGCTGGGC CTAGACCCAG GCGAGCTGCA GGGTGGCTCA 
GAGCTTAGCT CCAGCCCTGG TGTACACGTG AGGCAGACGA AAGAGGCGCC AGCCAAGCTG 
GAGTCCCAGG CTGGGGTACA GGTGTGCCGC AGGGCCCGGG ACCGGGTCCG CTCCATGTCA 
GGTGGCCATG GCCTGCGCGT GGGAGCCCTG GACACTCTAG CCTCCTACTT CCCTGGAGGC 
CCTATTTGTC CAGGGCCGCA GACCATGCCG GTCGTGAAGC CTCAGAAAGC CTCAGCGAGC 
ACCCAGATGC CAGTGCCAAA GGAAAAGACA AACCTGGCTG CCTACGTGCC CCTCCTGACC 
CAGGGCTGGG CTGAGATCCT GGTCCGGAGG CCCACAGGGA ACACCAGTTG GTTGATGAGC 
CTGGAGAATC CACTAAGCCC TTTCTCTTCG GACATCAACA ATATGCCACT GCAGGAGTTG 
TCCAATGCTC TCATGGCCGC TGAGCGCTTC AAGGAGCACC GTGACACGGC CCTGTACAAG 
TCATTGTCGG TGCCAGTGGC CAGCGCCTCC AAGCCCTCCC TCCTGCCCCG CTCCAACACA 
GACTCCCCGG GGGTTCTGGA GGAAGGATGT GCAGGTGAGA TGAACTTGTC CACTGAGCTT 
CCTGAGCTTG AGGACTTCAA GGCAGCCTCA GGTTCAGACA GGCGCTCCCA GCGTACCGAT 
GCTTACAGTA GGTCATCTTC GATCTCCAGC CAAGAGGAGA AGCAGCTGTG TGCAGAGGAG 
CTGGCTGCCA GGGGAATCCC CATTGAGCAG GTGGTGTCCT CCAGGGGTGG CCAGCCCTGT 
GTGGACCTCT CCTTTCAGCC CTCGCAGCCT CTAAGCAAAT CCAGCTCCTC ACCCGAACTG 
CAGACCTTGC AGGACATCTT TGGGGACGCT GGGGACAAGG CCGATGGAGG CTGGCTGAGC 
CCTGAGGCCA AGGCCCGGTG GCAGTCGGGG ACCCTGGATG GGGAGTGTGC TGCCTGGCTG 
GCTCCAGGTG AGGAGGGCCG GGCCCATGCC CCAGCCCAGC ACGAGGGCTC ACTGCCCTGC 
AGCTCCCCAC GCTCCCCACA CGGCCTGCGG CCCCGAGGCT ACACCATTTC CGACCCAGTC 
CAGTTGCGCA GGGGCAAGAC AGTGGAGAGA GACACCTCCA ACAGCCGAGC TGCTGCCTCC 
AACGCGGAGA AGGTGCCAGG CATCAACCCC AGCTTTGTGT TCCTGCAGCT CTACCACTCG 
CCCTTCTTTG GGGACGAGTC CAACAAGCCA ATTCTGTTAC CCAACGAGTC CTTCGAGCGC 
TCGGTGCAGC TCCTTGACCA GATCCCTTCG TATGACACAC ACAAGATCGC TGTTTTGTAC 
GTGGGAGAGG GCCAGAGCAA CAGTGAGCTC GCCATCCTCT CCAACGAGCA CGGCTCCTAC 
AGGTATACAG AATTCCTGAC GGGCCTGGGC AAGCTCATTG AGCTCAAGGA CTGCCAGCCT 
GACAAGGTGT ACCTGGGTGG CTTGGATGTG GGCGGCGAGG ACGGCCAGTT CACCTACTGC 
TGGCATGATG ACATCATGCA AGCAGTCTTT CACATCGCCA CCCTGATGCC CACCAAGGAT 
GGGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ATGACTTTGT GTCCATTGTC 
TACAACGACT CTGGCGAGGA CTTCAGGCTG GGCACCATCA AGGGCCAGTT CAACTTCGTG 
CATGTGATTA TCACTCCTCT GGACTATGAG TGCAACCTGG TCTCACTCCA GTGCCGGAAA 
GACATGGAGG GCCTGGTGGA CACCAGTATG GCCAAGATCG TGTCTGACTG TAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CTTGCATGCC AACATGGCCT CCCAGGTCCA CCACAGCCGC 
TCTAACCCCA CTGACATCTA CCCTTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGCGG 
CTACGTCACC GGATCCGGGA GGAAGCCAAT TACCTGAACC CTAGCCTGCC TCTGGTGCAG 
ATGCATCCTC CGGCCAACAC CAAGGCCCCT GCAGAGGCCA CACCCACCTA TGAAACCGGC 
CAGAGAAAGC GCCTCATCTC CTCAGTAGAC GACTTCACAG AATTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023413292.1
CDS	142..5292
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_023557524.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023557524.1

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGACA TGCCGTGGAA GCACTGTGGA AAGCTGTCGC GGACTTGCTG 
CAGCCAGACC GGCCACCAGA GGCACGACAT GCAGTGCTGG CCCTGCTCAA GGCCATCGTG 
CAGGGCCAGG GTGACCGCCT GGGTGTGCTC CGTGCCCTCT TCTTCAAGGT CATAAAGGAT 
TACCCTTCGA ACAAAGACTT GCACGAAAGG CTGGAGGTGT TCAAGGCCCT GACGGACAAT 
GGGAGACACA TAACCTACTT GGAAGAAGAG CTGGCTGAGT TTGTCCTGCA GTGGATGGAT 
GTGGGCTTAT CCTCAGAATT CCTGCTGGTG CTTGTGAACC TGGTCAAGTT CAACAGCTGT 
TACCTTGATG AATACATCGC ATCCATGGTT CACATGATCT GTCTGTTGTG CATCCGGACC 
TCGTCCTCCG TGGACATAGA GGTGTCCCTG CAGGTGCTGG ATGCCGTGGT CTGCTACAAC 
TGCCTGCCTG CCGCGAGCCT GCCCCTATTC ATTGTCACAT TGTGCCGCAC CGTCAACGTC 
AAGGAGCTCT GCGAGCCCTG CTGGAAGCTG ATGCGTAACC TCCTGGGAAC CCACCTGGGC 
CACAGTGCCA TCTACAACAT GTGCCGCATC ATGGAGGACA GAGCCTATAT GGAGGATGCC 
CCGCTGCTGA GAGGAGCTGT GTTCTTTGTC GGCATGGCGC TGTGGGGGGC CCACCGACTC 
TACTCCCTCA AGAACTCCCC TACATCTGTG CTGCCGTCGT TTTATGAGGC CATGACCTGC 
CCCAATGAGG TGGTGTCCTA CGAAATCGTC CTCTCTATCA CCAGACTCAT CAAGAAGTAC 
AGGAAGGAGC TGCAGGCTGT GACGTGGGAC ATCCTGCTGA GCATCATCGA ACGTCTGCTC 
CAGCAGCTCC TGACCCTCGA GAGTCAGGAG CTCAGAGCCA TCGTGCATGA CCTGCTGACG 
ACGGTGGAGG AGCTGTGTGA CCAGAATGAG TTCCACGGCT CACAGGAGAG ATACTTTGAG 
CTCATTGAGC GATGCGCCGA CCAGAGACCT GAGTCCTCCC TCTTGAACCT GATATCCTAC 
AGGGCACAGT CCATCCACCC AGCCAAGGAT GACTGGATCC ACAACCTGCA GCAGTTAATG 
GAGAGGTTCT TCAGGAACGA GTCCCGCAGT GCTGTGCGCA TCAAGGTGCT GGACATCCTG 
TCCTTTGTGC TGCTTATCAA CCGGCAGTTC TATGAGGAGG AGCTGATCAA CTCAGTGGTC 
ATCTCCCAAC TCTCCCACAT TCCAGAAGAC AGAGACCCAC AGGTCCGCAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC GGAAGGCTGC CACACCCACC ACTTCAATAA TCTTCTGGAC 
ATCATTGAGA AAGTGATTGC TCGCTCCCTG TTCCCTCCTC CTGAGCTAGA AGAAAGAGAT 
GTGCCTGCGT ACTCGGCCTC CTTGGAGGAT GTGAAGACTG CGGTCCTGGG GCTCCTGGTC 
ATCCTTCAGA CCAAGCTCTA CACTTTGCCT GCCAGCCATG CAGCACGGGT ATACGAGATG 
CTTGTCCACC ACATCCAGCT GCACTACAAG CACATGTACA CCTTGCCCAT TGCCAGCAGC 
ATCCGGCTTC AGGCCTTTGA CTTCCTGCTG CTGCTCCGGG CAGATGCACT ACACCGCCTG 
GGCCTGCCTA ACAAGGATGG TGCTGTACGG TTCAGCTCCT ACTGCCTCTG TGACCACATG 
GAGCCAGAGC ACGGCTTGGA GAAGAAGGCC AGTGGCCCCG TCTCGCCTCC CACGGGGCCT 
ACCGTCCCAG CACCTGCAGG CCCCACTGTG CGGCTTGGCT ATCTGCCCTA CTCCTTGCTC 
TTCCAAGTCC TGCTGCAGTG TCTGAAGCAG GAGACGGACT GGAAGGTGCT GAAGCTGGTA 
CTCAGCAAGC TGCCGGAGTC CCTGCGCTAC AAGGTCCTCA TCTTCACATC CCCCTGCAGC 
GTCGACCAGC TGTCCTCTGC CCTCTGCTCC ATGCTTTCAG GTCCCAAGAC CCTGGAACGA 
CTCCGAGGCA CACCAGAGGG CTTCTCTAGA ACCGACTTAC ACTTGGCAGT GGTTCCAGTG 
TTGACAGCAC TGATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTG 
TACTGCTTGG AGCAAGGCCT CATCTACCGC TGTGCCAGCC AATGTGTGGT GGCCCTGGCT 
GTGTGCAGTG TGGAGATGCC TGACGTCATC ATCAAGGCAC TGCCCGTACT GATTGTAAAG 
CTCACGCACA TCTCGGCCAC AGCCAGCATG GCCATCCCGC TGCTCGAGTT CCTGTCGACT 
CTGGCCCGGC TGCCGCATCT CTACAGGAAC TTTGCTGCTG AGCAGTACGC CAGCGTTTTC 
GCCATTTCAC TGCCCTACAC CAACCCATCC AAATTCAATC AGTATATTGT GTGCCTGGCC 
CACCACGTGA TAGCCATGTG GTTCATTCGG TGCCGCCTGC CCTTCCGGAA AGATTTTGTT 
CCGTATATCA CTAAGGGCCT TCGCTCCAAT GTCCTCCTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCACG GAGCACCAGT CTCAACGAGA GGCCCAAAAG TAGGATCCAG 
ACCTCCTTCA CCAGTGCCAG CTTGGGTTCT GCAGACGAAA ACTCGATGGC CCAGGCAGAC 
GACAACCTGA AAAATCTCCA CCTGGAGCTC ACCGAGACGT GCCTGGACAT GATGGCCAGA 
TACGTGTTCT CCAACTTCAC AGCTGTCCCC AAGAGGTCTC CCGTGGGAGA ATTCCTCCTG 
GCTGGAGGCA GGACCAAAAC CTGGCTGGTG GGGAACAAGC TGGTTACCGT AACGACGAGT 
GTGGGAACTG GCACACGGTC GCTGCTGGGC CTAGACCCAG GCGAGCTGCA GGGTGGCTCA 
GAGCTTAGCT CCAGCCCTGG TGTACACGTG AGGCAGACGA AAGAGGCGCC AGCCAAGCTG 
GAGTCCCAGG CTGGGGTACA GGTGTGCCGC AGGGCCCGGG ACCGGGTCCG CTCCATGTCA 
GGTGGCCATG GCCTGCGCGT GGGAGCCCTG GACACTCTAG CCTCCTACTT CCCTGGAGGC 
CCTATTTGTC CAGGGCCGCA GACCATGCCG GTCGTGAAGC CTCAGAAAGC CTCAGCGAGC 
ACCCAGATGC CAGTGCCAAA GGAAAAGACA AACCTGGCTG CCTACGTGCC CCTCCTGACC 
CAGGGCTGGG CTGAGATCCT GGTCCGGAGG CCCACAGGGA ACACCAGTTG GTTGATGAGC 
CTGGAGAATC CACTAAGCCC TTTCTCTTCG GACATCAACA ATATGCCACT GCAGGAGTTG 
TCCAATGCTC TCATGGCCGC TGAGCGCTTC AAGGAGCACC GTGACACGGC CCTGTACAAG 
TCATTGTCGG TGCCAGTGGC CAGCGCCTCC AAGCCCTCCC TCCTGCCCCG CTCCAACACA 
GACTCCCCGG GGGTTCTGGA GGAAGGATGT GCAGGTGAGA TGAACTTGTC CACTGAGCTT 
CCTGAGCTTG AGGACTTCAA GGCAGCCTCA GGTTCAGACA GGCGCTCCCA GCGTACCGAT 
GCTTACAGTA GGTCATCTTC GATCTCCAGC CAAGAGGAGA AGCAGCTGTG TGCAGAGGAG 
CTGGCTGCCA GGGGAATCCC CATTGAGCAG GTGGTGTCCT CCAGGGGTGG CCAGCCCTGT 
GTGGACCTCT CCTTTCAGCC CTCGCAGCCT CTAAGCAAAT CCAGCTCCTC ACCCGAACTG 
CAGACCTTGC AGGACATCTT TGGGGACGCT GGGGACAAGG CCGATGGAGG CTGGCTGAGC 
CCTGAGGCCA AGGCCCGGTG GCAGTCGGGG ACCCTGGATG GGGAGTGTGC TGCCTGGCTG 
GCTCCAGGTG AGGAGGGCCG GGCCCATGCC CCAGCCCAGC ACGAGGGCTC ACTGCCCTGC 
AGCTCCCCAC GCTCCCCACA CGGCCTGCGG CCCCGAGGCT ACACCATTTC CGACCCAGTC 
CAGTTGCGCA GGGGCAAGAC AGTGGAGAGA GACACCTCCA ACAGCCGAGC TGCTGCCTCC 
AACGCGGAGA AGGTGCCAGG CATCAACCCC AGCTTTGTGT TCCTGCAGCT CTACCACTCG 
CCCTTCTTTG GGGACGAGTC CAACAAGCCA ATTCTGTTAC CCAACGAGTC CTTCGAGCGC 
TCGGTGCAGC TCCTTGACCA GATCCCTTCG TATGACACAC ACAAGATCGC TGTTTTGTAC 
GTGGGAGAGG GCCAGAGCAA CAGTGAGCTC GCCATCCTCT CCAACGAGCA CGGCTCCTAC 
AGGTATACAG AATTCCTGAC GGGCCTGGGC AAGCTCATTG AGCTCAAGGA CTGCCAGCCT 
GACAAGGTGT ACCTGGGTGG CTTGGATGTG GGCGGCGAGG ACGGCCAGTT CACCTACTGC 
TGGCATGATG ACATCATGCA AGCAGTCTTT CACATCGCCA CCCTGATGCC CACCAAGGAT 
GGGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ATGACTTTGT GTCCATTGTC 
TACAACGACT CTGGCGAGGA CTTCAGGCTG GGCACCATCA AGGGCCAGTT CAACTTCGTG 
CATGTGATTA TCACTCCTCT GGACTATGAG TGCAACCTGG TCTCACTCCA GTGCCGGAAA 
GACATGGAGG GCCTGGTGGA CACCAGTATG GCCAAGATCG TGTCTGACTG TAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CTTGCATGCC AACATGGCCT CCCAGGTCCA CCACAGCCGC 
TCTAACCCCA CTGACATCTA CCCTTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGCGG 
CTACGTCACC GGATCCGGGA GGAAGCCAAT TACCTGAACC CTAGCCTGCC TCTGGTGCAG 
ATGCATCCTC CGGCCAACAC CAAGGCCCCT GCAGAGGCCA CACCCACCTA TGAAACCGGC 
CAGAGAAAGC GCCTCATCTC CTCAGTAGAC GACTTCACAG AATTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_023557526.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023413294.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OLo105848
Clone ID Related Accession (Same CDS sequence)	XM_023557526.1
Accession Version	XM_023557526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGAGCCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCC CGGGGGTTCT GGAGGAAGGA 
TGTGCAGGTG AGATGAACTT GTCCACTGAG CTTCCTGAGC TTGAGGACTT CAAGGCAGCC 
TCAGGTTCAG ACAGGCGCTC CCAGCGTACC GATGCTTACA GTAGGTCATC TTCGATCTCC 
AGCCAAGAGG AGAAGCAGCT GTGTGCAGAG GAGCTGGCTG CCAGGGGAAT CCCCATTGAG 
CAGGTGGTGT CCTCCAGGGG TGGCCAGCCC TGTGTGGACC TCTCCTTTCA GCCCTCGCAG 
CCTCTAAGCA AATCCAGCTC CTCACCCGAA CTGCAGACCT TGCAGGACAT CTTTGGGGAC 
GCTGGGGACA AGGCCGATGG AGGCTGGCTG AGCCCTGAGG CCAAGGCCCG GTGGCAGTCG 
GGGACCCTGG ATGGGGAGTG TGCTGCCTGG CTGGCTCCAG GTGAGGAGGG CCGGGCCCAT 
GCCCCAGCCC AGCACGAGGG CTCACTGCCC TGCAGCTCCC CACGCTCCCC ACACGGCCTG 
CGGCCCCGAG GCTACACCAT TTCCGACCCA GTCCAGTTGC GCAGGGGCAA GACAGTGGAG 
AGAGACACCT CCAACAGCCG AGCTGCTGCC TCCAACGCGG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTTG TGTTCCTGCA GCTCTACCAC TCGCCCTTCT TTGGGGACGA GTCCAACAAG 
CCAATTCTGT TACCCAACGA GTCCTTCGAG CGCTCGGTGC AGCTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT CGCTGTTTTG TACGTGGGAG AGGGCCAGAG CAACAGTGAG 
CTCGCCATCC TCTCCAACGA GCACGGCTCC TACAGGTATA CAGAATTCCT GACGGGCCTG 
GGCAAGCTCA TTGAGCTCAA GGACTGCCAG CCTGACAAGG TGTACCTGGG TGGCTTGGAT 
GTGGGCGGCG AGGACGGCCA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCAGTC 
TTTCACATCG CCACCCTGAT GCCCACCAAG GATGGGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGACTT TGTGTCCATT GTCTACAACG ACTCTGGCGA GGACTTCAGG 
CTGGGCACCA TCAAGGGCCA GTTCAACTTC GTGCATGTGA TTATCACTCC TCTGGACTAT 
GAGTGCAACC TGGTCTCACT CCAGTGCCGG AAAGACATGG AGGGCCTGGT GGACACCAGT 
ATGGCCAAGA TCGTGTCTGA CTGTAACCTG CCCTTCGTGG CCCGCCAGAT GGCCTTGCAT 
GCCAACATGG CCTCCCAGGT CCACCACAGC CGCTCTAACC CCACTGACAT CTACCCTTCC 
AAGTGGATTG CCCGGCTCCG CCACATCAAG CGGCTACGTC ACCGGATCCG GGAGGAAGCC 
AATTACCTGA ACCCTAGCCT GCCTCTGGTG CAGATGCATC CTCCGGCCAA CACCAAGGCC 
CCTGCAGAGG CCACACCCAC CTATGAAACC GGCCAGAGAA AGCGCCTCAT CTCCTCAGTA 
GACGACTTCA CAGAATTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023413294.1
CDS	142..5445
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTVASFSSLSQSSCQGKLHRSVSWADSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_023557526.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023557526.1

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGAGCCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCC CGGGGGTTCT GGAGGAAGGA 
TGTGCAGGTG AGATGAACTT GTCCACTGAG CTTCCTGAGC TTGAGGACTT CAAGGCAGCC 
TCAGGTTCAG ACAGGCGCTC CCAGCGTACC GATGCTTACA GTAGGTCATC TTCGATCTCC 
AGCCAAGAGG AGAAGCAGCT GTGTGCAGAG GAGCTGGCTG CCAGGGGAAT CCCCATTGAG 
CAGGTGGTGT CCTCCAGGGG TGGCCAGCCC TGTGTGGACC TCTCCTTTCA GCCCTCGCAG 
CCTCTAAGCA AATCCAGCTC CTCACCCGAA CTGCAGACCT TGCAGGACAT CTTTGGGGAC 
GCTGGGGACA AGGCCGATGG AGGCTGGCTG AGCCCTGAGG CCAAGGCCCG GTGGCAGTCG 
GGGACCCTGG ATGGGGAGTG TGCTGCCTGG CTGGCTCCAG GTGAGGAGGG CCGGGCCCAT 
GCCCCAGCCC AGCACGAGGG CTCACTGCCC TGCAGCTCCC CACGCTCCCC ACACGGCCTG 
CGGCCCCGAG GCTACACCAT TTCCGACCCA GTCCAGTTGC GCAGGGGCAA GACAGTGGAG 
AGAGACACCT CCAACAGCCG AGCTGCTGCC TCCAACGCGG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTTG TGTTCCTGCA GCTCTACCAC TCGCCCTTCT TTGGGGACGA GTCCAACAAG 
CCAATTCTGT TACCCAACGA GTCCTTCGAG CGCTCGGTGC AGCTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT CGCTGTTTTG TACGTGGGAG AGGGCCAGAG CAACAGTGAG 
CTCGCCATCC TCTCCAACGA GCACGGCTCC TACAGGTATA CAGAATTCCT GACGGGCCTG 
GGCAAGCTCA TTGAGCTCAA GGACTGCCAG CCTGACAAGG TGTACCTGGG TGGCTTGGAT 
GTGGGCGGCG AGGACGGCCA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCAGTC 
TTTCACATCG CCACCCTGAT GCCCACCAAG GATGGGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAATGACTT TGTGTCCATT GTCTACAACG ACTCTGGCGA GGACTTCAGG 
CTGGGCACCA TCAAGGGCCA GTTCAACTTC GTGCATGTGA TTATCACTCC TCTGGACTAT 
GAGTGCAACC TGGTCTCACT CCAGTGCCGG AAAGACATGG AGGGCCTGGT GGACACCAGT 
ATGGCCAAGA TCGTGTCTGA CTGTAACCTG CCCTTCGTGG CCCGCCAGAT GGCCTTGCAT 
GCCAACATGG CCTCCCAGGT CCACCACAGC CGCTCTAACC CCACTGACAT CTACCCTTCC 
AAGTGGATTG CCCGGCTCCG CCACATCAAG CGGCTACGTC ACCGGATCCG GGAGGAAGCC 
AATTACCTGA ACCCTAGCCT GCCTCTGGTG CAGATGCATC CTCCGGCCAA CACCAAGGCC 
CCTGCAGAGG CCACACCCAC CTATGAAACC GGCCAGAGAA AGCGCCTCAT CTCCTCAGTA 
GACGACTTCA CAGAATTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010597416.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010595718.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OLo49915
Clone ID Related Accession (Same CDS sequence)	XM_010597416.2 , XM_010597416.1
Accession Version	XM_010597416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 16, 2018 this sequence version replaced XM_010597416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGC AGTCTTTCAC 
ATCGCCACCC TGATGCCCAC CAAGGATGGG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATTGTCTAC AACGACTCTG GCGAGGACTT CAGGCTGGGC 
ACCATCAAGG GCCAGTTCAA CTTCGTGCAT GTGATTATCA CTCCTCTGGA CTATGAGTGC 
AACCTGGTCT CACTCCAGTG CCGGAAAGAC ATGGAGGGCC TGGTGGACAC CAGTATGGCC 
AAGATCGTGT CTGACTGTAA CCTGCCCTTC GTGGCCCGCC AGATGGCCTT GCATGCCAAC 
ATGGCCTCCC AGGTCCACCA CAGCCGCTCT AACCCCACTG ACATCTACCC TTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTA CGTCACCGGA TCCGGGAGGA AGCCAATTAC 
CTGAACCCTA GCCTGCCTCT GGTGCAGATG CATCCTCCGG CCAACACCAA GGCCCCTGCA 
GAGGCCACAC CCACCTATGA AACCGGCCAG AGAAAGCGCC TCATCTCCTC AGTAGACGAC 
TTCACAGAAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010595718.1
CDS	142..5379
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010597416.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010597416.2

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGC AGTCTTTCAC 
ATCGCCACCC TGATGCCCAC CAAGGATGGG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATTGTCTAC AACGACTCTG GCGAGGACTT CAGGCTGGGC 
ACCATCAAGG GCCAGTTCAA CTTCGTGCAT GTGATTATCA CTCCTCTGGA CTATGAGTGC 
AACCTGGTCT CACTCCAGTG CCGGAAAGAC ATGGAGGGCC TGGTGGACAC CAGTATGGCC 
AAGATCGTGT CTGACTGTAA CCTGCCCTTC GTGGCCCGCC AGATGGCCTT GCATGCCAAC 
ATGGCCTCCC AGGTCCACCA CAGCCGCTCT AACCCCACTG ACATCTACCC TTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTA CGTCACCGGA TCCGGGAGGA AGCCAATTAC 
CTGAACCCTA GCCTGCCTCT GGTGCAGATG CATCCTCCGG CCAACACCAA GGCCCCTGCA 
GAGGCCACAC CCACCTATGA AACCGGCCAG AGAAAGCGCC TCATCTCCTC AGTAGACGAC 
TTCACAGAAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010597416.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010595718.1 Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OLo49915
Clone ID Related Accession (Same CDS sequence)	XM_010597416.2 , XM_010597416.1
Accession Version	XM_010597416.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGC AGTCTTTCAC 
ATCGCCACCC TGATGCCCAC CAAGGATGGG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATTGTCTAC AACGACTCTG GCGAGGACTT CAGGCTGGGC 
ACCATCAAGG GCCAGTTCAA CTTCGTGCAT GTGATTATCA CTCCTCTGGA CTATGAGTGC 
AACCTGGTCT CACTCCAGTG CCGGAAAGAC ATGGAGGGCC TGGTGGACAC CAGTATGGCC 
AAGATCGTGT CTGACTGTAA CCTGCCCTTC GTGGCCCGCC AGATGGCCTT GCATGCCAAC 
ATGGCCTCCC AGGTCCACCA CAGCCGCTCT AACCCCACTG ACATCTACCC TTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTA CGTCACCGGA TCCGGGAGGA AGCCAATTAC 
CTGAACCCTA GCCTGCCTCT GGTGCAGATG CATCCTCCGG CCAACACCAA GGCCCCTGCA 
GAGGCCACAC CCACCTATGA AACCGGCCAG AGAAAGCGCC TCATCTCCTC AGTAGACGAC 
TTCACAGAAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010595718.1
CDS	22..5259
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010597416.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010597416.1

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTAG GATCCAGACC TCCTTCACCA GTGCCAGCTT GGGTTCTGCA 
GACGAAAACT CGATGGCCCA GGCAGACGAC AACCTGAAAA ATCTCCACCT GGAGCTCACC 
GAGACGTGCC TGGACATGAT GGCCAGATAC GTGTTCTCCA ACTTCACAGC TGTCCCCAAG 
AGGTCTCCCG TGGGAGAATT CCTCCTGGCT GGAGGCAGGA CCAAAACCTG GCTGGTGGGG 
AACAAGCTGG TTACCGTAAC GACGAGTGTG GGAACTGGCA CACGGTCGCT GCTGGGCCTA 
GACCCAGGCG AGCTGCAGGG TGGCTCAGAG CTTAGCTCCA GCCCTGGTGT ACACGTGAGG 
CAGACGAAAG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGGTACAGGT GTGCCGCAGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGT GGCCATGGCC TGCGCGTGGG AGCCCTGGAC 
ACTCTAGCCT CCTACTTCCC TGGAGGCCCT ATTTGTCCAG GGCCGCAGAC CATGCCGGTC 
GTGAAGCCTC AGAAAGCCTC AGCGAGCACC CAGATGCCAG TGCCAAAGGA AAAGACAAAC 
CTGGCTGCCT ACGTGCCCCT CCTGACCCAG GGCTGGGCTG AGATCCTGGT CCGGAGGCCC 
ACAGGGAACA CCAGTTGGTT GATGAGCCTG GAGAATCCAC TAAGCCCTTT CTCTTCGGAC 
ATCAACAATA TGCCACTGCA GGAGTTGTCC AATGCTCTCA TGGCCGCTGA GCGCTTCAAG 
GAGCACCGTG ACACGGCCCT GTACAAGTCA TTGTCGGTGC CAGTGGCCAG CGCCTCCAAG 
CCCTCCCTCC TGCCCCGCTC CAACACAGAC TCCCCGGGGG TTCTGGAGGA AGGATGTGCA 
GGTGAGATGA ACTTGTCCAC TGAGCTTCCT GAGCTTGAGG ACTTCAAGGC AGCCTCAGGT 
TCAGACAGGC GCTCCCAGCG TACCGATGCT TACAGTAGGT CATCTTCGAT CTCCAGCCAA 
GAGGAGAAGC AGCTGTGTGC AGAGGAGCTG GCTGCCAGGG GAATCCCCAT TGAGCAGGTG 
GTGTCCTCCA GGGGTGGCCA GCCCTGTGTG GACCTCTCCT TTCAGCCCTC GCAGCCTCTA 
AGCAAATCCA GCTCCTCACC CGAACTGCAG ACCTTGCAGG ACATCTTTGG GGACGCTGGG 
GACAAGGCCG ATGGAGGCTG GCTGAGCCCT GAGGCCAAGG CCCGGTGGCA GTCGGGGACC 
CTGGATGGGG AGTGTGCTGC CTGGCTGGCT CCAGGTGAGG AGGGCCGGGC CCATGCCCCA 
GCCCAGCACG AGGGCTCACT GCCCTGCAGC TCCCCACGCT CCCCACACGG CCTGCGGCCC 
CGAGGCTACA CCATTTCCGA CCCAGTCCAG TTGCGCAGGG GCAAGACAGT GGAGAGAGAC 
ACCTCCAACA GCCGAGCTGC TGCCTCCAAC GCGGAGAAGG TGCCAGGCAT CAACCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCGCCC TTCTTTGGGG ACGAGTCCAA CAAGCCAATT 
CTGTTACCCA ACGAGTCCTT CGAGCGCTCG GTGCAGCTCC TTGACCAGAT CCCTTCGTAT 
GACACACACA AGATCGCTGT TTTGTACGTG GGAGAGGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTCTCCA ACGAGCACGG CTCCTACAGG TATACAGAAT TCCTGACGGG CCTGGGCAAG 
CTCATTGAGC TCAAGGACTG CCAGCCTGAC AAGGTGTACC TGGGTGGCTT GGATGTGGGC 
GGCGAGGACG GCCAGTTCAC CTACTGCTGG CATGATGACA TCATGCAAGC AGTCTTTCAC 
ATCGCCACCC TGATGCCCAC CAAGGATGGG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATTGTCTAC AACGACTCTG GCGAGGACTT CAGGCTGGGC 
ACCATCAAGG GCCAGTTCAA CTTCGTGCAT GTGATTATCA CTCCTCTGGA CTATGAGTGC 
AACCTGGTCT CACTCCAGTG CCGGAAAGAC ATGGAGGGCC TGGTGGACAC CAGTATGGCC 
AAGATCGTGT CTGACTGTAA CCTGCCCTTC GTGGCCCGCC AGATGGCCTT GCATGCCAAC 
ATGGCCTCCC AGGTCCACCA CAGCCGCTCT AACCCCACTG ACATCTACCC TTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTA CGTCACCGGA TCCGGGAGGA AGCCAATTAC 
CTGAACCCTA GCCTGCCTCT GGTGCAGATG CATCCTCCGG CCAACACCAA GGCCCCTGCA 
GAGGCCACAC CCACCTATGA AACCGGCCAG AGAAAGCGCC TCATCTCCTC AGTAGACGAC 
TTCACAGAAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010597417.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010595719.1 Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OLo49916
Clone ID Related Accession (Same CDS sequence)	XM_010597417.2 , XM_010597417.1
Accession Version	XM_010597417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 16, 2018 this sequence version replaced XM_010597417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGACTCC CCGGGGGTTC TGGAGGAAGG ATGTGCAGGT 
GAGATGAACT TGTCCACTGA GCTTCCTGAG CTTGAGGACT TCAAGGCAGC CTCAGGTTCA 
GACAGGCGCT CCCAGCGTAC CGATGCTTAC AGTAGGTCAT CTTCGATCTC CAGCCAAGAG 
GAGAAGCAGC TGTGTGCAGA GGAGCTGGCT GCCAGGGGAA TCCCCATTGA GCAGGTGGTG 
TCCTCCAGGG GTGGCCAGCC CTGTGTGGAC CTCTCCTTTC AGCCCTCGCA GCCTCTAAGC 
AAATCCAGCT CCTCACCCGA ACTGCAGACC TTGCAGGACA TCTTTGGGGA CGCTGGGGAC 
AAGGCCGATG GAGGCTGGCT GAGCCCTGAG GCCAAGGCCC GGTGGCAGTC GGGGACCCTG 
GATGGGGAGT GTGCTGCCTG GCTGGCTCCA GGTGAGGAGG GCCGGGCCCA TGCCCCAGCC 
CAGCACGAGG GCTCACTGCC CTGCAGCTCC CCACGCTCCC CACACGGCCT GCGGCCCCGA 
GGCTACACCA TTTCCGACCC AGTCCAGTTG CGCAGGGGCA AGACAGTGGA GAGAGACACC 
TCCAACAGCC GAGCTGCTGC CTCCAACGCG GAGAAGGTGC CAGGCATCAA CCCCAGCTTT 
GTGTTCCTGC AGCTCTACCA CTCGCCCTTC TTTGGGGACG AGTCCAACAA GCCAATTCTG 
TTACCCAACG AGTCCTTCGA GCGCTCGGTG CAGCTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TCGCTGTTTT GTACGTGGGA GAGGGCCAGA GCAACAGTGA GCTCGCCATC 
CTCTCCAACG AGCACGGCTC CTACAGGTAT ACAGAATTCC TGACGGGCCT GGGCAAGCTC 
ATTGAGCTCA AGGACTGCCA GCCTGACAAG GTGTACCTGG GTGGCTTGGA TGTGGGCGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010595719.1
CDS	142..5376
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010597417.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010597417.2

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGACTCC CCGGGGGTTC TGGAGGAAGG ATGTGCAGGT 
GAGATGAACT TGTCCACTGA GCTTCCTGAG CTTGAGGACT TCAAGGCAGC CTCAGGTTCA 
GACAGGCGCT CCCAGCGTAC CGATGCTTAC AGTAGGTCAT CTTCGATCTC CAGCCAAGAG 
GAGAAGCAGC TGTGTGCAGA GGAGCTGGCT GCCAGGGGAA TCCCCATTGA GCAGGTGGTG 
TCCTCCAGGG GTGGCCAGCC CTGTGTGGAC CTCTCCTTTC AGCCCTCGCA GCCTCTAAGC 
AAATCCAGCT CCTCACCCGA ACTGCAGACC TTGCAGGACA TCTTTGGGGA CGCTGGGGAC 
AAGGCCGATG GAGGCTGGCT GAGCCCTGAG GCCAAGGCCC GGTGGCAGTC GGGGACCCTG 
GATGGGGAGT GTGCTGCCTG GCTGGCTCCA GGTGAGGAGG GCCGGGCCCA TGCCCCAGCC 
CAGCACGAGG GCTCACTGCC CTGCAGCTCC CCACGCTCCC CACACGGCCT GCGGCCCCGA 
GGCTACACCA TTTCCGACCC AGTCCAGTTG CGCAGGGGCA AGACAGTGGA GAGAGACACC 
TCCAACAGCC GAGCTGCTGC CTCCAACGCG GAGAAGGTGC CAGGCATCAA CCCCAGCTTT 
GTGTTCCTGC AGCTCTACCA CTCGCCCTTC TTTGGGGACG AGTCCAACAA GCCAATTCTG 
TTACCCAACG AGTCCTTCGA GCGCTCGGTG CAGCTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TCGCTGTTTT GTACGTGGGA GAGGGCCAGA GCAACAGTGA GCTCGCCATC 
CTCTCCAACG AGCACGGCTC CTACAGGTAT ACAGAATTCC TGACGGGCCT GGGCAAGCTC 
ATTGAGCTCA AGGACTGCCA GCCTGACAAG GTGTACCTGG GTGGCTTGGA TGTGGGCGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010597417.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010595719.1 Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OLo49916
Clone ID Related Accession (Same CDS sequence)	XM_010597417.2 , XM_010597417.1
Accession Version	XM_010597417.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGACTCC CCGGGGGTTC TGGAGGAAGG ATGTGCAGGT 
GAGATGAACT TGTCCACTGA GCTTCCTGAG CTTGAGGACT TCAAGGCAGC CTCAGGTTCA 
GACAGGCGCT CCCAGCGTAC CGATGCTTAC AGTAGGTCAT CTTCGATCTC CAGCCAAGAG 
GAGAAGCAGC TGTGTGCAGA GGAGCTGGCT GCCAGGGGAA TCCCCATTGA GCAGGTGGTG 
TCCTCCAGGG GTGGCCAGCC CTGTGTGGAC CTCTCCTTTC AGCCCTCGCA GCCTCTAAGC 
AAATCCAGCT CCTCACCCGA ACTGCAGACC TTGCAGGACA TCTTTGGGGA CGCTGGGGAC 
AAGGCCGATG GAGGCTGGCT GAGCCCTGAG GCCAAGGCCC GGTGGCAGTC GGGGACCCTG 
GATGGGGAGT GTGCTGCCTG GCTGGCTCCA GGTGAGGAGG GCCGGGCCCA TGCCCCAGCC 
CAGCACGAGG GCTCACTGCC CTGCAGCTCC CCACGCTCCC CACACGGCCT GCGGCCCCGA 
GGCTACACCA TTTCCGACCC AGTCCAGTTG CGCAGGGGCA AGACAGTGGA GAGAGACACC 
TCCAACAGCC GAGCTGCTGC CTCCAACGCG GAGAAGGTGC CAGGCATCAA CCCCAGCTTT 
GTGTTCCTGC AGCTCTACCA CTCGCCCTTC TTTGGGGACG AGTCCAACAA GCCAATTCTG 
TTACCCAACG AGTCCTTCGA GCGCTCGGTG CAGCTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TCGCTGTTTT GTACGTGGGA GAGGGCCAGA GCAACAGTGA GCTCGCCATC 
CTCTCCAACG AGCACGGCTC CTACAGGTAT ACAGAATTCC TGACGGGCCT GGGCAAGCTC 
ATTGAGCTCA AGGACTGCCA GCCTGACAAG GTGTACCTGG GTGGCTTGGA TGTGGGCGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010595719.1
CDS	22..5256
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPHPRSAEGRQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPDRPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNKDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTSSSVDIEVSLQVLDAVVCYNCLPAASLPLFIVTLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLSIIERLLQQLLTLESQELRAIVHDLLTTVEELCDQNEFHGSQERYFELIERCADQRPESSLLNLISYRAQSIHPAKDDWIHNLQQLMERFFRNESRSAVRIKVLDILSFVLLINRQFYEEELINSVVISQLSHIPEDRDPQVRKLATQLLVDLAEGCHTHHFNNLLDIIEKVIARSLFPPPELEERDVPAYSASLEDVKTAVLGLLVILQTKLYTLPASHAARVYEMLVHHIQLHYKHMYTLPIASSIRLQAFDFLLLLRADALHRLGLPNKDGAVRFSSYCLCDHMEPEHGLEKKASGPVSPPTGPTVPAPAGPTVRLGYLPYSLLFQVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVIIKALPVLIVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSFTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDPGELQGGSELSSSPGVHVRQTKEAPAKLESQAGVQVCRRARDRVRSMSGGHGLRVGALDTLASYFPGGPICPGPQTMPVVKPQKASASTQMPVPKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPVASASKPSLLPRSNTDSPGVLEEGCAGEMNLSTELPELEDFKAASGSDRRSQRTDAYSRSSSISSQEEKQLCAEELAARGIPIEQVVSSRGGQPCVDLSFQPSQPLSKSSSSPELQTLQDIFGDAGDKADGGWLSPEAKARWQSGTLDGECAAWLAPGEEGRAHAPAQHEGSLPCSSPRSPHGLRPRGYTISDPVQLRRGKTVERDTSNSRAAASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVGGEDGQFTYCWHDDIMQAVFHIATLMPTKDGDKHRCDKKRHLGNDFVSIVYNDSGEDFRLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDCNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEANYLNPSLPLVQMHPPANTKAPAEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010597417.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010597417.1

ORF Insert Sequence:

ATGGCCAAGC CCACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTCCTGGGC 
CTGGGAACAC CAAGGCCACA TCCCAGGTCT GCAGAGGGCA GACAGACGGA GTTTATCATC 
ACAGCAGAAA TCCTGAGAGA ACTGAGTGTC GAGTGTGGTC TGAACAATCG CATCCGGGTC 
ATAGGGCAGA TCTGTGAAGT AGCAAAAACT AAGAAGTTTG AGGAGCATGC CGTGGAAGCA 
CTGTGGAAAG CTGTCGCGGA CTTGCTGCAG CCAGACCGGC CACCAGAGGC ACGACATGCA 
GTGCTGGCCC TGCTCAAGGC CATCGTGCAG GGCCAGGGTG ACCGCCTGGG TGTGCTCCGT 
GCCCTCTTCT TCAAGGTCAT AAAGGATTAC CCTTCGAACA AAGACTTGCA CGAAAGGCTG 
GAGGTGTTCA AGGCCCTGAC GGACAATGGG AGACACATAA CCTACTTGGA AGAAGAGCTG 
GCTGAGTTTG TCCTGCAGTG GATGGATGTG GGCTTATCCT CAGAATTCCT GCTGGTGCTT 
GTGAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGATGAAT ACATCGCATC CATGGTTCAC 
ATGATCTGTC TGTTGTGCAT CCGGACCTCG TCCTCCGTGG ACATAGAGGT GTCCCTGCAG 
GTGCTGGATG CCGTGGTCTG CTACAACTGC CTGCCTGCCG CGAGCCTGCC CCTATTCATT 
GTCACATTGT GCCGCACCGT CAACGTCAAG GAGCTCTGCG AGCCCTGCTG GAAGCTGATG 
CGTAACCTCC TGGGAACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAG CCTATATGGA GGATGCCCCG CTGCTGAGAG GAGCTGTGTT CTTTGTCGGC 
ATGGCGCTGT GGGGGGCCCA CCGACTCTAC TCCCTCAAGA ACTCCCCTAC ATCTGTGCTG 
CCGTCGTTTT ATGAGGCCAT GACCTGCCCC AATGAGGTGG TGTCCTACGA AATCGTCCTC 
TCTATCACCA GACTCATCAA GAAGTACAGG AAGGAGCTGC AGGCTGTGAC GTGGGACATC 
CTGCTGAGCA TCATCGAACG TCTGCTCCAG CAGCTCCTGA CCCTCGAGAG TCAGGAGCTC 
AGAGCCATCG TGCATGACCT GCTGACGACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGCTCAC AGGAGAGATA CTTTGAGCTC ATTGAGCGAT GCGCCGACCA GAGACCTGAG 
TCCTCCCTCT TGAACCTGAT ATCCTACAGG GCACAGTCCA TCCACCCAGC CAAGGATGAC 
TGGATCCACA ACCTGCAGCA GTTAATGGAG AGGTTCTTCA GGAACGAGTC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CATCCTGTCC TTTGTGCTGC TTATCAACCG GCAGTTCTAT 
GAGGAGGAGC TGATCAACTC AGTGGTCATC TCCCAACTCT CCCACATTCC AGAAGACAGA 
GACCCACAGG TCCGCAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAATAATCT TCTGGACATC ATTGAGAAAG TGATTGCTCG CTCCCTGTTC 
CCTCCTCCTG AGCTAGAAGA AAGAGATGTG CCTGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC TTTGCCTGCC 
AGCCATGCAG CACGGGTATA CGAGATGCTT GTCCACCACA TCCAGCTGCA CTACAAGCAC 
ATGTACACCT TGCCCATTGC CAGCAGCATC CGGCTTCAGG CCTTTGACTT CCTGCTGCTG 
CTCCGGGCAG ATGCACTACA CCGCCTGGGC CTGCCTAACA AGGATGGTGC TGTACGGTTC 
AGCTCCTACT GCCTCTGTGA CCACATGGAG CCAGAGCACG GCTTGGAGAA GAAGGCCAGT 
GGCCCCGTCT CGCCTCCCAC GGGGCCTACC GTCCCAGCAC CTGCAGGCCC CACTGTGCGG 
CTTGGCTATC TGCCCTACTC CTTGCTCTTC CAAGTCCTGC TGCAGTGTCT GAAGCAGGAG 
ACGGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CGGAGTCCCT GCGCTACAAG 
GTCCTCATCT TCACATCCCC CTGCAGCGTC GACCAGCTGT CCTCTGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAACGACTC CGAGGCACAC CAGAGGGCTT CTCTAGAACC 
GACTTACACT TGGCAGTGGT TCCAGTGTTG ACAGCACTGA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTGTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAAT GTGTGGTGGC CCTGGCTGTG TGCAGTGTGG AGATGCCTGA CGTCATCATC 
AAGGCACTGC CCGTACTGAT TGTAAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC 
ATCCCGCTGC TCGAGTTCCT GTCGACTCTG GCCCGGCTGC CGCATCTCTA CAGGAACTTT 
GCTGCTGAGC AGTACGCCAG CGTTTTCGCC ATTTCACTGC CCTACACCAA CCCATCCAAA 
TTCAATCAGT ATATTGTGTG CCTGGCCCAC CACGTGATAG CCATGTGGTT CATTCGGTGC 
CGCCTGCCCT TCCGGAAAGA TTTTGTTCCG TATATCACTA AGGGCCTTCG CTCCAATGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT CCAGACCTCC TTCACCAGTG CCAGCTTGGG TTCTGCAGAC 
GAAAACTCGA TGGCCCAGGC AGACGACAAC CTGAAAAATC TCCACCTGGA GCTCACCGAG 
ACGTGCCTGG ACATGATGGC CAGATACGTG TTCTCCAACT TCACAGCTGT CCCCAAGAGG 
TCTCCCGTGG GAGAATTCCT CCTGGCTGGA GGCAGGACCA AAACCTGGCT GGTGGGGAAC 
AAGCTGGTTA CCGTAACGAC GAGTGTGGGA ACTGGCACAC GGTCGCTGCT GGGCCTAGAC 
CCAGGCGAGC TGCAGGGTGG CTCAGAGCTT AGCTCCAGCC CTGGTGTACA CGTGAGGCAG 
ACGAAAGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGG TACAGGTGTG CCGCAGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGTGGC CATGGCCTGC GCGTGGGAGC CCTGGACACT 
CTAGCCTCCT ACTTCCCTGG AGGCCCTATT TGTCCAGGGC CGCAGACCAT GCCGGTCGTG 
AAGCCTCAGA AAGCCTCAGC GAGCACCCAG ATGCCAGTGC CAAAGGAAAA GACAAACCTG 
GCTGCCTACG TGCCCCTCCT GACCCAGGGC TGGGCTGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GTTGGTTGAT GAGCCTGGAG AATCCACTAA GCCCTTTCTC TTCGGACATC 
AACAATATGC CACTGCAGGA GTTGTCCAAT GCTCTCATGG CCGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCCCTGTA CAAGTCATTG TCGGTGCCAG TGGCCAGCGC CTCCAAGCCC 
TCCCTCCTGC CCCGCTCCAA CACAGACTCC CCGGGGGTTC TGGAGGAAGG ATGTGCAGGT 
GAGATGAACT TGTCCACTGA GCTTCCTGAG CTTGAGGACT TCAAGGCAGC CTCAGGTTCA 
GACAGGCGCT CCCAGCGTAC CGATGCTTAC AGTAGGTCAT CTTCGATCTC CAGCCAAGAG 
GAGAAGCAGC TGTGTGCAGA GGAGCTGGCT GCCAGGGGAA TCCCCATTGA GCAGGTGGTG 
TCCTCCAGGG GTGGCCAGCC CTGTGTGGAC CTCTCCTTTC AGCCCTCGCA GCCTCTAAGC 
AAATCCAGCT CCTCACCCGA ACTGCAGACC TTGCAGGACA TCTTTGGGGA CGCTGGGGAC 
AAGGCCGATG GAGGCTGGCT GAGCCCTGAG GCCAAGGCCC GGTGGCAGTC GGGGACCCTG 
GATGGGGAGT GTGCTGCCTG GCTGGCTCCA GGTGAGGAGG GCCGGGCCCA TGCCCCAGCC 
CAGCACGAGG GCTCACTGCC CTGCAGCTCC CCACGCTCCC CACACGGCCT GCGGCCCCGA 
GGCTACACCA TTTCCGACCC AGTCCAGTTG CGCAGGGGCA AGACAGTGGA GAGAGACACC 
TCCAACAGCC GAGCTGCTGC CTCCAACGCG GAGAAGGTGC CAGGCATCAA CCCCAGCTTT 
GTGTTCCTGC AGCTCTACCA CTCGCCCTTC TTTGGGGACG AGTCCAACAA GCCAATTCTG 
TTACCCAACG AGTCCTTCGA GCGCTCGGTG CAGCTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TCGCTGTTTT GTACGTGGGA GAGGGCCAGA GCAACAGTGA GCTCGCCATC 
CTCTCCAACG AGCACGGCTC CTACAGGTAT ACAGAATTCC TGACGGGCCT GGGCAAGCTC 
ATTGAGCTCA AGGACTGCCA GCCTGACAAG GTGTACCTGG GTGGCTTGGA TGTGGGCGGC 
GAGGACGGCC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCAGT CTTTCACATC 
GCCACCCTGA TGCCCACCAA GGATGGGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAATGACT TTGTGTCCAT TGTCTACAAC GACTCTGGCG AGGACTTCAG GCTGGGCACC 
ATCAAGGGCC AGTTCAACTT CGTGCATGTG ATTATCACTC CTCTGGACTA TGAGTGCAAC 
CTGGTCTCAC TCCAGTGCCG GAAAGACATG GAGGGCCTGG TGGACACCAG TATGGCCAAG 
ATCGTGTCTG ACTGTAACCT GCCCTTCGTG GCCCGCCAGA TGGCCTTGCA TGCCAACATG 
GCCTCCCAGG TCCACCACAG CCGCTCTAAC CCCACTGACA TCTACCCTTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTACGT CACCGGATCC GGGAGGAAGC CAATTACCTG 
AACCCTAGCC TGCCTCTGGT GCAGATGCAT CCTCCGGCCA ACACCAAGGC CCCTGCAGAG 
GCCACACCCA CCTATGAAAC CGGCCAGAGA AAGCGCCTCA TCTCCTCAGT AGACGACTTC 
ACAGAATTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TSC2 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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