MTHFD1 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD1
Entrez Gene ID	100661814
Full Name	methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
Gene Type	protein-coding
Organism	Loxodonta africana(African savanna elephant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010588685.1	XP_010586987.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_010588686.2	XP_010586988.2	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_010588685.2	XP_010586987.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
XM_010588686.1	XP_010586988.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2

Pathways from BioSystems
Gene Ontology

The following MTHFD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLo37369	XM_010588685.2 Latest version!	Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLo37370	XM_010588686.1 Latest version!	Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLo97951	XM_010588686.2 Latest version!	Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OLo37369	XM_010588685.1	Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD1 ( XM_010588685.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010586987.1 Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLo37369
Clone ID Related Accession (Same CDS sequence)	XM_010588685.1 , XM_010588685.2
Accession Version	XM_010588685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG ATCGGTCTCC TGCAGCCAGA GAAAGGGGTC TCGGATACAA CTGCGTTTGC 
AGGCAAATAA GGGAGAGACT GAAGAACCAA GTCACTCAGA TGAAGGAGCA AGTACCTGGG 
TTCACACCAG GCCTAGCAAT TTTGCAGGTT GGCAACAGAG ATGATTCAAA TCTTTATATA 
AATGTGAAGC TGAAGGCTGC TGAAGAGATT GGGATAAAAG CCACTCACAC TAAGTTACCA 
AGAACAGCCA CAGAATCCGA GGTGTTAAAG TGCATCATAT CTTTGAATGA AGACTCCACT 
GTGCATGGAT TGCTAGTGCA GCTCCCTTTA GACTCAGAGA ATCCCATTAA CACTGAAACA 
GTGATCAATA CCATTGTACC CGAAAAGGAC GTGGATGGAT TGACTAGCAT CAGTGCTGGA 
AAACTTGCTA GAGGTGACCT AAATGACTGT TTCATTCCTT GTACACCTAA AGGATGCTTG 
GAACTCATCA AAGAGACAGG GGTGCAGATT GCCGGAAGGC ATGCTGTGGT TGTTGGGCGC 
AGTAAAATAG TTGGTGCCCC GATGCATGAC TTGCTTCTGT GGAACAATGC CACAGTGACC 
ACTTGCCACT CAAAGACTGC CAATCTGGAT GAGGAGGTAA GTAAAGGTGA TATCCTGGTG 
GTTGCAACTG GTCGGCCAGA AATGGTGAAA GGGGAGTGGA TTAAACCCGG GGCAGTCGTC 
ATCGACTGTG GAATCAATTA TGTTCCAGAT GATACAAAAC CGAACGGGAG AAAAGTTGTG 
GGTGACGTGG CATACAACGA GGCCAAAGAG AGGGCAGGCT TCATCACCCC TGTTCCTGGC 
GGTGTCGGGC CCATGACGGT GGCAATGCTA ATGCAGAGCA CAGTGGAGAG TGCAAAACGT 
TTCCTGGAGA AATTTAAGCC GGGAGAGTGG GTGATTCAGT ATAACAGCCT TAACCTCAAG 
ACACCTGTCC CTAGTGACAT TGATATATCA CGAGCTTACA AACCAAAGCC CATTGGTAAC 
CTGGCTCGAG AAATTGGTCT GCTCTCTGAA GAGGTGGAAT TGTATGGTGA AACCAAGGCC 
AAGGTTCTGC TTTCAGCGCT AGAACGCCTC AGGCATCAGC CTGATGGAAA ATATGTGGTG 
GTGACGGGAA TAACTCCAAC ACCCCTGGGA GAAGGGAAAA GCACGACGAC AATTGGGCTG 
GTACAAGCCC TTGGTGCCTA CCTTCATCAG AATGTATTTG CGTGTGTACG ACAGCCTTCT 
CAGGGCCCGA CCTTTGGAAT AAAAGGTGGC GCTGCAGGAG GTGGCTACTC GCAGGTTATT 
CCCATGGAAG AGTTTAATCT CCACCTCACA GGGGACATCC ATGCCATCAC TGCAGCTAAT 
AACCTCGTTG CTGCAGCCAT CGATGCCCGG ATGTTCCATG AACTGACACA GACAGACAAG 
GCTCTCTTTA ATCGTTTGGT ACCATCAGTA AATGGAGTGA GAAAGTTCTC TGATATTCAA 
ATTCGAAGGC TACGGAGACT AGGCATTGAA AAGACCGACC CTACCACACT GACAGATGAA 
GAGATAAACA GATTTGCAAG ACTGGATATT GATCCGGAAA CCATAACTTG GCAGAGAGTG 
TTGGATACCA ATGATAGATT CCTGAGGAAG ATCACAATTG GACAGGCTCC AACGGAGAAA 
GGGTACACGC GGACGGCCCA GTTTGATATC TCTGTGGCCA GTGAAATCAT GGCTGTCCTG 
GCTCTCACCA CTTCTCTAGA AGACATGAGA GAGAGACTGG GCAAAATGGT GGTAGCTTCC 
AACAAGAAAG GGGAGCCCAT CAGTGCTGAA GACCTGGGGG TGAGTGGTGC ATTGACGGTG 
CTTATGAAGG ATGCTATCAA GCCCAACCTC ATGCAGACCC TGGAGGGCAC TCCAGTGTTT 
GTTCATGCTG GACCGTTTGC CAACATTGCA CATGGGAATT CCTCTATCAT AGCAGACCGG 
ATTGCACTCA AACTTGTTGG CCCTGAAGGG TTTGTAGTGA CTGAAGCAGG ATTCGGAGCC 
GACATTGGAA TGGAAAAGTT TTTTAACATC AAATGCCGAT ATTCTGGTCT CCGCCCTCAC 
GTTGTGGTGC TTGTTGCCAC TGTCAGGGCT CTTAAAATGC ACGGGGGCGG CCCCACGGTC 
ACGGCTGGAC TGCCTCTGCC CAAGGCTTAC GCAGAGGAGA ACCTGGAGCT GGTTGAAAAA 
GGCTTCAGTA ACTTGAAGAA ACAAATTGAA AACGCCAAAC TCTTCGGAGT CCCGGTAGTG 
GTGGCTGTGA ATGTATTCAA GACGGACACG GAGGCTGAGC TGGACCTCGT CAGCCGCCTT 
GCCAAAGAAC ATGGGGCTTT TGATGCTGTG AAGTGCACTC ACTGGGCAGA AGGGGGCAAG 
GGAGCCTTAG CCCTGGCTAA CGCCGTCCAG AGAGCAGCAC AGGCCCCCAG CAGCTTCCAG 
CTCCTTTATG ACCTCAAGCT CCCAGTTGAG GATAAGATCA GGATCATCGC ACAGAAAATC 
TACGGGGCAG ACGACATTGA ATTGCTCCCC GAAGCCCAGC AAAAAGCCGA AGTCTACACG 
AAGCAGGGCT TTGGGGATCT ACCCATCTGC ATGGCGAAAA CACACTTGTC CCTGTCTCAT 
AACCCAGAGC TAAAAGGTGT GCCGAAAGGC TTTGTGCTGC CCATCCGCGA CATCCGCGCC 
AGCGTGGGGG CTGGTTTTCT GTACCCCTTA GTAGGAACGA TGAGCACGAT GCCTGGACTC 
CCCACCCGGC CCTGTTTCTA TGATATTGAT TTGGACCCTG AAACCAAGCA GGTGAATGGG 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010586987.1
CDS	127..2955
Translation	MPEDRSPAARERGLGYNCVCRQIRERLKNQVTQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAEEIGIKATHTKLPRTATESEVLKCIISLNEDSTVHGLLVQLPLDSENPINTETVINTIVPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVSKGDILVVATGRPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYNEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGEWVIQYNSLNLKTPVPSDIDISRAYKPKPIGNLAREIGLLSEEVELYGETKAKVLLSALERLRHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAYLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGYTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASNKKGEPISAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYAEENLELVEKGFSNLKKQIENAKLFGVPVVVAVNVFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALANAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQQKAEVYTKQGFGDLPICMAKTHLSLSHNPELKGVPKGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETKQVNGLF

Target ORF information:

RefSeq Version	XM_010588685.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010588685.1

ORF Insert Sequence:

ATGCCTGAAG ATCGGTCTCC TGCAGCCAGA GAAAGGGGTC TCGGATACAA CTGCGTTTGC 
AGGCAAATAA GGGAGAGACT GAAGAACCAA GTCACTCAGA TGAAGGAGCA AGTACCTGGG 
TTCACACCAG GCCTAGCAAT TTTGCAGGTT GGCAACAGAG ATGATTCAAA TCTTTATATA 
AATGTGAAGC TGAAGGCTGC TGAAGAGATT GGGATAAAAG CCACTCACAC TAAGTTACCA 
AGAACAGCCA CAGAATCCGA GGTGTTAAAG TGCATCATAT CTTTGAATGA AGACTCCACT 
GTGCATGGAT TGCTAGTGCA GCTCCCTTTA GACTCAGAGA ATCCCATTAA CACTGAAACA 
GTGATCAATA CCATTGTACC CGAAAAGGAC GTGGATGGAT TGACTAGCAT CAGTGCTGGA 
AAACTTGCTA GAGGTGACCT AAATGACTGT TTCATTCCTT GTACACCTAA AGGATGCTTG 
GAACTCATCA AAGAGACAGG GGTGCAGATT GCCGGAAGGC ATGCTGTGGT TGTTGGGCGC 
AGTAAAATAG TTGGTGCCCC GATGCATGAC TTGCTTCTGT GGAACAATGC CACAGTGACC 
ACTTGCCACT CAAAGACTGC CAATCTGGAT GAGGAGGTAA GTAAAGGTGA TATCCTGGTG 
GTTGCAACTG GTCGGCCAGA AATGGTGAAA GGGGAGTGGA TTAAACCCGG GGCAGTCGTC 
ATCGACTGTG GAATCAATTA TGTTCCAGAT GATACAAAAC CGAACGGGAG AAAAGTTGTG 
GGTGACGTGG CATACAACGA GGCCAAAGAG AGGGCAGGCT TCATCACCCC TGTTCCTGGC 
GGTGTCGGGC CCATGACGGT GGCAATGCTA ATGCAGAGCA CAGTGGAGAG TGCAAAACGT 
TTCCTGGAGA AATTTAAGCC GGGAGAGTGG GTGATTCAGT ATAACAGCCT TAACCTCAAG 
ACACCTGTCC CTAGTGACAT TGATATATCA CGAGCTTACA AACCAAAGCC CATTGGTAAC 
CTGGCTCGAG AAATTGGTCT GCTCTCTGAA GAGGTGGAAT TGTATGGTGA AACCAAGGCC 
AAGGTTCTGC TTTCAGCGCT AGAACGCCTC AGGCATCAGC CTGATGGAAA ATATGTGGTG 
GTGACGGGAA TAACTCCAAC ACCCCTGGGA GAAGGGAAAA GCACGACGAC AATTGGGCTG 
GTACAAGCCC TTGGTGCCTA CCTTCATCAG AATGTATTTG CGTGTGTACG ACAGCCTTCT 
CAGGGCCCGA CCTTTGGAAT AAAAGGTGGC GCTGCAGGAG GTGGCTACTC GCAGGTTATT 
CCCATGGAAG AGTTTAATCT CCACCTCACA GGGGACATCC ATGCCATCAC TGCAGCTAAT 
AACCTCGTTG CTGCAGCCAT CGATGCCCGG ATGTTCCATG AACTGACACA GACAGACAAG 
GCTCTCTTTA ATCGTTTGGT ACCATCAGTA AATGGAGTGA GAAAGTTCTC TGATATTCAA 
ATTCGAAGGC TACGGAGACT AGGCATTGAA AAGACCGACC CTACCACACT GACAGATGAA 
GAGATAAACA GATTTGCAAG ACTGGATATT GATCCGGAAA CCATAACTTG GCAGAGAGTG 
TTGGATACCA ATGATAGATT CCTGAGGAAG ATCACAATTG GACAGGCTCC AACGGAGAAA 
GGGTACACGC GGACGGCCCA GTTTGATATC TCTGTGGCCA GTGAAATCAT GGCTGTCCTG 
GCTCTCACCA CTTCTCTAGA AGACATGAGA GAGAGACTGG GCAAAATGGT GGTAGCTTCC 
AACAAGAAAG GGGAGCCCAT CAGTGCTGAA GACCTGGGGG TGAGTGGTGC ATTGACGGTG 
CTTATGAAGG ATGCTATCAA GCCCAACCTC ATGCAGACCC TGGAGGGCAC TCCAGTGTTT 
GTTCATGCTG GACCGTTTGC CAACATTGCA CATGGGAATT CCTCTATCAT AGCAGACCGG 
ATTGCACTCA AACTTGTTGG CCCTGAAGGG TTTGTAGTGA CTGAAGCAGG ATTCGGAGCC 
GACATTGGAA TGGAAAAGTT TTTTAACATC AAATGCCGAT ATTCTGGTCT CCGCCCTCAC 
GTTGTGGTGC TTGTTGCCAC TGTCAGGGCT CTTAAAATGC ACGGGGGCGG CCCCACGGTC 
ACGGCTGGAC TGCCTCTGCC CAAGGCTTAC GCAGAGGAGA ACCTGGAGCT GGTTGAAAAA 
GGCTTCAGTA ACTTGAAGAA ACAAATTGAA AACGCCAAAC TCTTCGGAGT CCCGGTAGTG 
GTGGCTGTGA ATGTATTCAA GACGGACACG GAGGCTGAGC TGGACCTCGT CAGCCGCCTT 
GCCAAAGAAC ATGGGGCTTT TGATGCTGTG AAGTGCACTC ACTGGGCAGA AGGGGGCAAG 
GGAGCCTTAG CCCTGGCTAA CGCCGTCCAG AGAGCAGCAC AGGCCCCCAG CAGCTTCCAG 
CTCCTTTATG ACCTCAAGCT CCCAGTTGAG GATAAGATCA GGATCATCGC ACAGAAAATC 
TACGGGGCAG ACGACATTGA ATTGCTCCCC GAAGCCCAGC AAAAAGCCGA AGTCTACACG 
AAGCAGGGCT TTGGGGATCT ACCCATCTGC ATGGCGAAAA CACACTTGTC CCTGTCTCAT 
AACCCAGAGC TAAAAGGTGT GCCGAAAGGC TTTGTGCTGC CCATCCGCGA CATCCGCGCC 
AGCGTGGGGG CTGGTTTTCT GTACCCCTTA GTAGGAACGA TGAGCACGAT GCCTGGACTC 
CCCACCCGGC CCTGTTTCTA TGATATTGAT TTGGACCCTG AAACCAAGCA GGTGAATGGG 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_010588685.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010586987.1 Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLo37369
Clone ID Related Accession (Same CDS sequence)	XM_010588685.1 , XM_010588685.2
Accession Version	XM_010588685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 16, 2018 this sequence version replaced XM_010588685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG ATCGGTCTCC TGCAGCCAGA GAAAGGGGTC TCGGATACAA CTGCGTTTGC 
AGGCAAATAA GGGAGAGACT GAAGAACCAA GTCACTCAGA TGAAGGAGCA AGTACCTGGG 
TTCACACCAG GCCTAGCAAT TTTGCAGGTT GGCAACAGAG ATGATTCAAA TCTTTATATA 
AATGTGAAGC TGAAGGCTGC TGAAGAGATT GGGATAAAAG CCACTCACAC TAAGTTACCA 
AGAACAGCCA CAGAATCCGA GGTGTTAAAG TGCATCATAT CTTTGAATGA AGACTCCACT 
GTGCATGGAT TGCTAGTGCA GCTCCCTTTA GACTCAGAGA ATCCCATTAA CACTGAAACA 
GTGATCAATA CCATTGTACC CGAAAAGGAC GTGGATGGAT TGACTAGCAT CAGTGCTGGA 
AAACTTGCTA GAGGTGACCT AAATGACTGT TTCATTCCTT GTACACCTAA AGGATGCTTG 
GAACTCATCA AAGAGACAGG GGTGCAGATT GCCGGAAGGC ATGCTGTGGT TGTTGGGCGC 
AGTAAAATAG TTGGTGCCCC GATGCATGAC TTGCTTCTGT GGAACAATGC CACAGTGACC 
ACTTGCCACT CAAAGACTGC CAATCTGGAT GAGGAGGTAA GTAAAGGTGA TATCCTGGTG 
GTTGCAACTG GTCGGCCAGA AATGGTGAAA GGGGAGTGGA TTAAACCCGG GGCAGTCGTC 
ATCGACTGTG GAATCAATTA TGTTCCAGAT GATACAAAAC CGAACGGGAG AAAAGTTGTG 
GGTGACGTGG CATACAACGA GGCCAAAGAG AGGGCAGGCT TCATCACCCC TGTTCCTGGC 
GGTGTCGGGC CCATGACGGT GGCAATGCTA ATGCAGAGCA CAGTGGAGAG TGCAAAACGT 
TTCCTGGAGA AATTTAAGCC GGGAGAGTGG GTGATTCAGT ATAACAGCCT TAACCTCAAG 
ACACCTGTCC CTAGTGACAT TGATATATCA CGAGCTTACA AACCAAAGCC CATTGGTAAC 
CTGGCTCGAG AAATTGGTCT GCTCTCTGAA GAGGTGGAAT TGTATGGTGA AACCAAGGCC 
AAGGTTCTGC TTTCAGCGCT AGAACGCCTC AGGCATCAGC CTGATGGAAA ATATGTGGTG 
GTGACGGGAA TAACTCCAAC ACCCCTGGGA GAAGGGAAAA GCACGACGAC AATTGGGCTG 
GTACAAGCCC TTGGTGCCTA CCTTCATCAG AATGTATTTG CGTGTGTACG ACAGCCTTCT 
CAGGGCCCGA CCTTTGGAAT AAAAGGTGGC GCTGCAGGAG GTGGCTACTC GCAGGTTATT 
CCCATGGAAG AGTTTAATCT CCACCTCACA GGGGACATCC ATGCCATCAC TGCAGCTAAT 
AACCTCGTTG CTGCAGCCAT CGATGCCCGG ATGTTCCATG AACTGACACA GACAGACAAG 
GCTCTCTTTA ATCGTTTGGT ACCATCAGTA AATGGAGTGA GAAAGTTCTC TGATATTCAA 
ATTCGAAGGC TACGGAGACT AGGCATTGAA AAGACCGACC CTACCACACT GACAGATGAA 
GAGATAAACA GATTTGCAAG ACTGGATATT GATCCGGAAA CCATAACTTG GCAGAGAGTG 
TTGGATACCA ATGATAGATT CCTGAGGAAG ATCACAATTG GACAGGCTCC AACGGAGAAA 
GGGTACACGC GGACGGCCCA GTTTGATATC TCTGTGGCCA GTGAAATCAT GGCTGTCCTG 
GCTCTCACCA CTTCTCTAGA AGACATGAGA GAGAGACTGG GCAAAATGGT GGTAGCTTCC 
AACAAGAAAG GGGAGCCCAT CAGTGCTGAA GACCTGGGGG TGAGTGGTGC ATTGACGGTG 
CTTATGAAGG ATGCTATCAA GCCCAACCTC ATGCAGACCC TGGAGGGCAC TCCAGTGTTT 
GTTCATGCTG GACCGTTTGC CAACATTGCA CATGGGAATT CCTCTATCAT AGCAGACCGG 
ATTGCACTCA AACTTGTTGG CCCTGAAGGG TTTGTAGTGA CTGAAGCAGG ATTCGGAGCC 
GACATTGGAA TGGAAAAGTT TTTTAACATC AAATGCCGAT ATTCTGGTCT CCGCCCTCAC 
GTTGTGGTGC TTGTTGCCAC TGTCAGGGCT CTTAAAATGC ACGGGGGCGG CCCCACGGTC 
ACGGCTGGAC TGCCTCTGCC CAAGGCTTAC GCAGAGGAGA ACCTGGAGCT GGTTGAAAAA 
GGCTTCAGTA ACTTGAAGAA ACAAATTGAA AACGCCAAAC TCTTCGGAGT CCCGGTAGTG 
GTGGCTGTGA ATGTATTCAA GACGGACACG GAGGCTGAGC TGGACCTCGT CAGCCGCCTT 
GCCAAAGAAC ATGGGGCTTT TGATGCTGTG AAGTGCACTC ACTGGGCAGA AGGGGGCAAG 
GGAGCCTTAG CCCTGGCTAA CGCCGTCCAG AGAGCAGCAC AGGCCCCCAG CAGCTTCCAG 
CTCCTTTATG ACCTCAAGCT CCCAGTTGAG GATAAGATCA GGATCATCGC ACAGAAAATC 
TACGGGGCAG ACGACATTGA ATTGCTCCCC GAAGCCCAGC AAAAAGCCGA AGTCTACACG 
AAGCAGGGCT TTGGGGATCT ACCCATCTGC ATGGCGAAAA CACACTTGTC CCTGTCTCAT 
AACCCAGAGC TAAAAGGTGT GCCGAAAGGC TTTGTGCTGC CCATCCGCGA CATCCGCGCC 
AGCGTGGGGG CTGGTTTTCT GTACCCCTTA GTAGGAACGA TGAGCACGAT GCCTGGACTC 
CCCACCCGGC CCTGTTTCTA TGATATTGAT TTGGACCCTG AAACCAAGCA GGTGAATGGG 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010586987.1
CDS	143..2971
Translation	MPEDRSPAARERGLGYNCVCRQIRERLKNQVTQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAEEIGIKATHTKLPRTATESEVLKCIISLNEDSTVHGLLVQLPLDSENPINTETVINTIVPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVSKGDILVVATGRPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYNEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGEWVIQYNSLNLKTPVPSDIDISRAYKPKPIGNLAREIGLLSEEVELYGETKAKVLLSALERLRHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAYLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGYTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASNKKGEPISAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYAEENLELVEKGFSNLKKQIENAKLFGVPVVVAVNVFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALANAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQQKAEVYTKQGFGDLPICMAKTHLSLSHNPELKGVPKGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETKQVNGLF

Target ORF information:

RefSeq Version	XM_010588685.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010588685.2

ORF Insert Sequence:

ATGCCTGAAG ATCGGTCTCC TGCAGCCAGA GAAAGGGGTC TCGGATACAA CTGCGTTTGC 
AGGCAAATAA GGGAGAGACT GAAGAACCAA GTCACTCAGA TGAAGGAGCA AGTACCTGGG 
TTCACACCAG GCCTAGCAAT TTTGCAGGTT GGCAACAGAG ATGATTCAAA TCTTTATATA 
AATGTGAAGC TGAAGGCTGC TGAAGAGATT GGGATAAAAG CCACTCACAC TAAGTTACCA 
AGAACAGCCA CAGAATCCGA GGTGTTAAAG TGCATCATAT CTTTGAATGA AGACTCCACT 
GTGCATGGAT TGCTAGTGCA GCTCCCTTTA GACTCAGAGA ATCCCATTAA CACTGAAACA 
GTGATCAATA CCATTGTACC CGAAAAGGAC GTGGATGGAT TGACTAGCAT CAGTGCTGGA 
AAACTTGCTA GAGGTGACCT AAATGACTGT TTCATTCCTT GTACACCTAA AGGATGCTTG 
GAACTCATCA AAGAGACAGG GGTGCAGATT GCCGGAAGGC ATGCTGTGGT TGTTGGGCGC 
AGTAAAATAG TTGGTGCCCC GATGCATGAC TTGCTTCTGT GGAACAATGC CACAGTGACC 
ACTTGCCACT CAAAGACTGC CAATCTGGAT GAGGAGGTAA GTAAAGGTGA TATCCTGGTG 
GTTGCAACTG GTCGGCCAGA AATGGTGAAA GGGGAGTGGA TTAAACCCGG GGCAGTCGTC 
ATCGACTGTG GAATCAATTA TGTTCCAGAT GATACAAAAC CGAACGGGAG AAAAGTTGTG 
GGTGACGTGG CATACAACGA GGCCAAAGAG AGGGCAGGCT TCATCACCCC TGTTCCTGGC 
GGTGTCGGGC CCATGACGGT GGCAATGCTA ATGCAGAGCA CAGTGGAGAG TGCAAAACGT 
TTCCTGGAGA AATTTAAGCC GGGAGAGTGG GTGATTCAGT ATAACAGCCT TAACCTCAAG 
ACACCTGTCC CTAGTGACAT TGATATATCA CGAGCTTACA AACCAAAGCC CATTGGTAAC 
CTGGCTCGAG AAATTGGTCT GCTCTCTGAA GAGGTGGAAT TGTATGGTGA AACCAAGGCC 
AAGGTTCTGC TTTCAGCGCT AGAACGCCTC AGGCATCAGC CTGATGGAAA ATATGTGGTG 
GTGACGGGAA TAACTCCAAC ACCCCTGGGA GAAGGGAAAA GCACGACGAC AATTGGGCTG 
GTACAAGCCC TTGGTGCCTA CCTTCATCAG AATGTATTTG CGTGTGTACG ACAGCCTTCT 
CAGGGCCCGA CCTTTGGAAT AAAAGGTGGC GCTGCAGGAG GTGGCTACTC GCAGGTTATT 
CCCATGGAAG AGTTTAATCT CCACCTCACA GGGGACATCC ATGCCATCAC TGCAGCTAAT 
AACCTCGTTG CTGCAGCCAT CGATGCCCGG ATGTTCCATG AACTGACACA GACAGACAAG 
GCTCTCTTTA ATCGTTTGGT ACCATCAGTA AATGGAGTGA GAAAGTTCTC TGATATTCAA 
ATTCGAAGGC TACGGAGACT AGGCATTGAA AAGACCGACC CTACCACACT GACAGATGAA 
GAGATAAACA GATTTGCAAG ACTGGATATT GATCCGGAAA CCATAACTTG GCAGAGAGTG 
TTGGATACCA ATGATAGATT CCTGAGGAAG ATCACAATTG GACAGGCTCC AACGGAGAAA 
GGGTACACGC GGACGGCCCA GTTTGATATC TCTGTGGCCA GTGAAATCAT GGCTGTCCTG 
GCTCTCACCA CTTCTCTAGA AGACATGAGA GAGAGACTGG GCAAAATGGT GGTAGCTTCC 
AACAAGAAAG GGGAGCCCAT CAGTGCTGAA GACCTGGGGG TGAGTGGTGC ATTGACGGTG 
CTTATGAAGG ATGCTATCAA GCCCAACCTC ATGCAGACCC TGGAGGGCAC TCCAGTGTTT 
GTTCATGCTG GACCGTTTGC CAACATTGCA CATGGGAATT CCTCTATCAT AGCAGACCGG 
ATTGCACTCA AACTTGTTGG CCCTGAAGGG TTTGTAGTGA CTGAAGCAGG ATTCGGAGCC 
GACATTGGAA TGGAAAAGTT TTTTAACATC AAATGCCGAT ATTCTGGTCT CCGCCCTCAC 
GTTGTGGTGC TTGTTGCCAC TGTCAGGGCT CTTAAAATGC ACGGGGGCGG CCCCACGGTC 
ACGGCTGGAC TGCCTCTGCC CAAGGCTTAC GCAGAGGAGA ACCTGGAGCT GGTTGAAAAA 
GGCTTCAGTA ACTTGAAGAA ACAAATTGAA AACGCCAAAC TCTTCGGAGT CCCGGTAGTG 
GTGGCTGTGA ATGTATTCAA GACGGACACG GAGGCTGAGC TGGACCTCGT CAGCCGCCTT 
GCCAAAGAAC ATGGGGCTTT TGATGCTGTG AAGTGCACTC ACTGGGCAGA AGGGGGCAAG 
GGAGCCTTAG CCCTGGCTAA CGCCGTCCAG AGAGCAGCAC AGGCCCCCAG CAGCTTCCAG 
CTCCTTTATG ACCTCAAGCT CCCAGTTGAG GATAAGATCA GGATCATCGC ACAGAAAATC 
TACGGGGCAG ACGACATTGA ATTGCTCCCC GAAGCCCAGC AAAAAGCCGA AGTCTACACG 
AAGCAGGGCT TTGGGGATCT ACCCATCTGC ATGGCGAAAA CACACTTGTC CCTGTCTCAT 
AACCCAGAGC TAAAAGGTGT GCCGAAAGGC TTTGTGCTGC CCATCCGCGA CATCCGCGCC 
AGCGTGGGGG CTGGTTTTCT GTACCCCTTA GTAGGAACGA TGAGCACGAT GCCTGGACTC 
CCCACCCGGC CCTGTTTCTA TGATATTGAT TTGGACCCTG AAACCAAGCA GGTGAATGGG 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_010588686.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010586988.1 Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLo37370
Clone ID Related Accession (Same CDS sequence)	XM_010588686.1
Accession Version	XM_010588686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG CAGGAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
AAGAACCAAG TCACTCAGAT GAAGGAGCAA GTACCTGGGT TCACACCAGG CCTAGCAATT 
TTGCAGGTTG GCAACAGAGA TGATTCAAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATAAAAGC CACTCACACT AAGTTACCAA GAACAGCCAC AGAATCCGAG 
GTGTTAAAGT GCATCATATC TTTGAATGAA GACTCCACTG TGCATGGATT GCTAGTGCAG 
CTCCCTTTAG ACTCAGAGAA TCCCATTAAC ACTGAAACAG TGATCAATAC CATTGTACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGACCTA 
AATGACTGTT TCATTCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CCGGAAGGCA TGCTGTGGTT GTTGGGCGCA GTAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CTTGCCACTC AAAGACTGCC 
AATCTGGATG AGGAGGTAAG TAAAGGTGAT ATCCTGGTGG TTGCAACTGG TCGGCCAGAA 
ATGGTGAAAG GGGAGTGGAT TAAACCCGGG GCAGTCGTCA TCGACTGTGG AATCAATTAT 
GTTCCAGATG ATACAAAACC GAACGGGAGA AAAGTTGTGG GTGACGTGGC ATACAACGAG 
GCCAAAGAGA GGGCAGGCTT CATCACCCCT GTTCCTGGCG GTGTCGGGCC CATGACGGTG 
GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCAAAACGTT TCCTGGAGAA ATTTAAGCCG 
GGAGAGTGGG TGATTCAGTA TAACAGCCTT AACCTCAAGA CACCTGTCCC TAGTGACATT 
GATATATCAC GAGCTTACAA ACCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTCTCTGAAG AGGTGGAATT GTATGGTGAA ACCAAGGCCA AGGTTCTGCT TTCAGCGCTA 
GAACGCCTCA GGCATCAGCC TGATGGAAAA TATGTGGTGG TGACGGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACGACGACA ATTGGGCTGG TACAAGCCCT TGGTGCCTAC 
CTTCATCAGA ATGTATTTGC GTGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCG CAGGTTATTC CCATGGAAGA GTTTAATCTC 
CACCTCACAG GGGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACACAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATTCAAA TTCGAAGGCT ACGGAGACTA 
GGCATTGAAA AGACCGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ATCCGGAAAC CATAACTTGG CAGAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGGCTCCA ACGGAGAAAG GGTACACGCG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTAGCTTCCA ACAAGAAAGG GGAGCCCATC 
AGTGCTGAAG ACCTGGGGGT GAGTGGTGCA TTGACGGTGC TTATGAAGGA TGCTATCAAG 
CCCAACCTCA TGCAGACCCT GGAGGGCACT CCAGTGTTTG TTCATGCTGG ACCGTTTGCC 
AACATTGCAC ATGGGAATTC CTCTATCATA GCAGACCGGA TTGCACTCAA ACTTGTTGGC 
CCTGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCCG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGATA TTCTGGTCTC CGCCCTCACG TTGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TTAAAATGCA CGGGGGCGGC CCCACGGTCA CGGCTGGACT GCCTCTGCCC 
AAGGCTTACG CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ACGCCAAACT CTTCGGAGTC CCGGTAGTGG TGGCTGTGAA TGTATTCAAG 
ACGGACACGG AGGCTGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT 
GATGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GAGCCTTAGC CCTGGCTAAC 
GCCGTCCAGA GAGCAGCACA GGCCCCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATCGCA CAGAAAATCT ACGGGGCAGA CGACATTGAA 
TTGCTCCCCG AAGCCCAGCA AAAAGCCGAA GTCTACACGA AGCAGGGCTT TGGGGATCTA 
CCCATCTGCA TGGCGAAAAC ACACTTGTCC CTGTCTCATA ACCCAGAGCT AAAAGGTGTG 
CCGAAAGGCT TTGTGCTGCC CATCCGCGAC ATCCGCGCCA GCGTGGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACGATG CCTGGACTCC CCACCCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACCAAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010586988.1
CDS	117..2924
Translation	MAPAGILNGKVVSAQIRERLKNQVTQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAEEIGIKATHTKLPRTATESEVLKCIISLNEDSTVHGLLVQLPLDSENPINTETVINTIVPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVSKGDILVVATGRPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYNEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGEWVIQYNSLNLKTPVPSDIDISRAYKPKPIGNLAREIGLLSEEVELYGETKAKVLLSALERLRHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAYLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGYTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASNKKGEPISAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYAEENLELVEKGFSNLKKQIENAKLFGVPVVVAVNVFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALANAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQQKAEVYTKQGFGDLPICMAKTHLSLSHNPELKGVPKGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETKQVNGLF

Target ORF information:

RefSeq Version	XM_010588686.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010588686.1

ORF Insert Sequence:

ATGGCGCCGG CAGGAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
AAGAACCAAG TCACTCAGAT GAAGGAGCAA GTACCTGGGT TCACACCAGG CCTAGCAATT 
TTGCAGGTTG GCAACAGAGA TGATTCAAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATAAAAGC CACTCACACT AAGTTACCAA GAACAGCCAC AGAATCCGAG 
GTGTTAAAGT GCATCATATC TTTGAATGAA GACTCCACTG TGCATGGATT GCTAGTGCAG 
CTCCCTTTAG ACTCAGAGAA TCCCATTAAC ACTGAAACAG TGATCAATAC CATTGTACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGACCTA 
AATGACTGTT TCATTCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CCGGAAGGCA TGCTGTGGTT GTTGGGCGCA GTAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CTTGCCACTC AAAGACTGCC 
AATCTGGATG AGGAGGTAAG TAAAGGTGAT ATCCTGGTGG TTGCAACTGG TCGGCCAGAA 
ATGGTGAAAG GGGAGTGGAT TAAACCCGGG GCAGTCGTCA TCGACTGTGG AATCAATTAT 
GTTCCAGATG ATACAAAACC GAACGGGAGA AAAGTTGTGG GTGACGTGGC ATACAACGAG 
GCCAAAGAGA GGGCAGGCTT CATCACCCCT GTTCCTGGCG GTGTCGGGCC CATGACGGTG 
GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCAAAACGTT TCCTGGAGAA ATTTAAGCCG 
GGAGAGTGGG TGATTCAGTA TAACAGCCTT AACCTCAAGA CACCTGTCCC TAGTGACATT 
GATATATCAC GAGCTTACAA ACCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTCTCTGAAG AGGTGGAATT GTATGGTGAA ACCAAGGCCA AGGTTCTGCT TTCAGCGCTA 
GAACGCCTCA GGCATCAGCC TGATGGAAAA TATGTGGTGG TGACGGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACGACGACA ATTGGGCTGG TACAAGCCCT TGGTGCCTAC 
CTTCATCAGA ATGTATTTGC GTGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCG CAGGTTATTC CCATGGAAGA GTTTAATCTC 
CACCTCACAG GGGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACACAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATTCAAA TTCGAAGGCT ACGGAGACTA 
GGCATTGAAA AGACCGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ATCCGGAAAC CATAACTTGG CAGAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGGCTCCA ACGGAGAAAG GGTACACGCG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTAGCTTCCA ACAAGAAAGG GGAGCCCATC 
AGTGCTGAAG ACCTGGGGGT GAGTGGTGCA TTGACGGTGC TTATGAAGGA TGCTATCAAG 
CCCAACCTCA TGCAGACCCT GGAGGGCACT CCAGTGTTTG TTCATGCTGG ACCGTTTGCC 
AACATTGCAC ATGGGAATTC CTCTATCATA GCAGACCGGA TTGCACTCAA ACTTGTTGGC 
CCTGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCCG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGATA TTCTGGTCTC CGCCCTCACG TTGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TTAAAATGCA CGGGGGCGGC CCCACGGTCA CGGCTGGACT GCCTCTGCCC 
AAGGCTTACG CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ACGCCAAACT CTTCGGAGTC CCGGTAGTGG TGGCTGTGAA TGTATTCAAG 
ACGGACACGG AGGCTGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT 
GATGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GAGCCTTAGC CCTGGCTAAC 
GCCGTCCAGA GAGCAGCACA GGCCCCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATCGCA CAGAAAATCT ACGGGGCAGA CGACATTGAA 
TTGCTCCCCG AAGCCCAGCA AAAAGCCGAA GTCTACACGA AGCAGGGCTT TGGGGATCTA 
CCCATCTGCA TGGCGAAAAC ACACTTGTCC CTGTCTCATA ACCCAGAGCT AAAAGGTGTG 
CCGAAAGGCT TTGTGCTGCC CATCCGCGAC ATCCGCGCCA GCGTGGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACGATG CCTGGACTCC CCACCCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACCAAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_010588686.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010586988.2 Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLo97951
Clone ID Related Accession (Same CDS sequence)	XM_010588686.2
Accession Version	XM_010588686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 16, 2018 this sequence version replaced XM_010588686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCCG CCAGCCTATC ATCACCCACT TCCTTCACAT TGCCTCCTCC TTCGGCCCGC 
CGTATCACAG GCCCCCTACA GTATTTTGTT AGTAACCCTT CGTCCCTTCA GACTGCGCAC 
GCTCAGTCAC GGACGTCGGA GCGCATGCGC AGTAGCGTGA TCCCCGGGTG GGCGCTTAGC 
ACGTGGGCTA GGCTGTCCCT TTTGGCCACA GAAGGACGTA GGACCTCGCT TTTGTTGGTG 
TCCATCGCGC GCAGCGGACT AGTAAGGGCC ATGGCGCCGG CAGGAATCCT GAACGGGAAG 
GTGGTCTCCG CGCAAATAAG GGAGAGACTG AAGAACCAAG TCACTCAGAT GAAGGAGCAA 
GTACCTGGGT TCACACCAGG CCTAGCAATT TTGCAGGTTG GCAACAGAGA TGATTCAAAT 
CTTTATATAA ATGTGAAGCT GAAGGCTGCT GAAGAGATTG GGATAAAAGC CACTCACACT 
AAGTTACCAA GAACAGCCAC AGAATCCGAG GTGTTAAAGT GCATCATATC TTTGAATGAA 
GACTCCACTG TGCATGGATT GCTAGTGCAG CTCCCTTTAG ACTCAGAGAA TCCCATTAAC 
ACTGAAACAG TGATCAATAC CATTGTACCC GAAAAGGACG TGGATGGATT GACTAGCATC 
AGTGCTGGAA AACTTGCTAG AGGTGACCTA AATGACTGTT TCATTCCTTG TACACCTAAA 
GGATGCTTGG AACTCATCAA AGAGACAGGG GTGCAGATTG CCGGAAGGCA TGCTGTGGTT 
GTTGGGCGCA GTAAAATAGT TGGTGCCCCG ATGCATGACT TGCTTCTGTG GAACAATGCC 
ACAGTGACCA CTTGCCACTC AAAGACTGCC AATCTGGATG AGGAGGTAAG TAAAGGTGAT 
ATCCTGGTGG TTGCAACTGG TCGGCCAGAA ATGGTGAAAG GGGAGTGGAT TAAACCCGGG 
GCAGTCGTCA TCGACTGTGG AATCAATTAT GTTCCAGATG ATACAAAACC GAACGGGAGA 
AAAGTTGTGG GTGACGTGGC ATACAACGAG GCCAAAGAGA GGGCAGGCTT CATCACCCCT 
GTTCCTGGCG GTGTCGGGCC CATGACGGTG GCAATGCTAA TGCAGAGCAC AGTGGAGAGT 
GCAAAACGTT TCCTGGAGAA ATTTAAGCCG GGAGAGTGGG TGATTCAGTA TAACAGCCTT 
AACCTCAAGA CACCTGTCCC TAGTGACATT GATATATCAC GAGCTTACAA ACCAAAGCCC 
ATTGGTAACC TGGCTCGAGA AATTGGTCTG CTCTCTGAAG AGGTGGAATT GTATGGTGAA 
ACCAAGGCCA AGGTTCTGCT TTCAGCGCTA GAACGCCTCA GGCATCAGCC TGATGGAAAA 
TATGTGGTGG TGACGGGAAT AACTCCAACA CCCCTGGGAG AAGGGAAAAG CACGACGACA 
ATTGGGCTGG TACAAGCCCT TGGTGCCTAC CTTCATCAGA ATGTATTTGC GTGTGTACGA 
CAGCCTTCTC AGGGCCCGAC CTTTGGAATA AAAGGTGGCG CTGCAGGAGG TGGCTACTCG 
CAGGTTATTC CCATGGAAGA GTTTAATCTC CACCTCACAG GGGACATCCA TGCCATCACT 
GCAGCTAATA ACCTCGTTGC TGCAGCCATC GATGCCCGGA TGTTCCATGA ACTGACACAG 
ACAGACAAGG CTCTCTTTAA TCGTTTGGTA CCATCAGTAA ATGGAGTGAG AAAGTTCTCT 
GATATTCAAA TTCGAAGGCT ACGGAGACTA GGCATTGAAA AGACCGACCC TACCACACTG 
ACAGATGAAG AGATAAACAG ATTTGCAAGA CTGGATATTG ATCCGGAAAC CATAACTTGG 
CAGAGAGTGT TGGATACCAA TGATAGATTC CTGAGGAAGA TCACAATTGG ACAGGCTCCA 
ACGGAGAAAG GGTACACGCG GACGGCCCAG TTTGATATCT CTGTGGCCAG TGAAATCATG 
GCTGTCCTGG CTCTCACCAC TTCTCTAGAA GACATGAGAG AGAGACTGGG CAAAATGGTG 
GTAGCTTCCA ACAAGAAAGG GGAGCCCATC AGTGCTGAAG ACCTGGGGGT GAGTGGTGCA 
TTGACGGTGC TTATGAAGGA TGCTATCAAG CCCAACCTCA TGCAGACCCT GGAGGGCACT 
CCAGTGTTTG TTCATGCTGG ACCGTTTGCC AACATTGCAC ATGGGAATTC CTCTATCATA 
GCAGACCGGA TTGCACTCAA ACTTGTTGGC CCTGAAGGGT TTGTAGTGAC TGAAGCAGGA 
TTCGGAGCCG ACATTGGAAT GGAAAAGTTT TTTAACATCA AATGCCGATA TTCTGGTCTC 
CGCCCTCACG TTGTGGTGCT TGTTGCCACT GTCAGGGCTC TTAAAATGCA CGGGGGCGGC 
CCCACGGTCA CGGCTGGACT GCCTCTGCCC AAGGCTTACG CAGAGGAGAA CCTGGAGCTG 
GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA CAAATTGAAA ACGCCAAACT CTTCGGAGTC 
CCGGTAGTGG TGGCTGTGAA TGTATTCAAG ACGGACACGG AGGCTGAGCT GGACCTCGTC 
AGCCGCCTTG CCAAAGAACA TGGGGCTTTT GATGCTGTGA AGTGCACTCA CTGGGCAGAA 
GGGGGCAAGG GAGCCTTAGC CCTGGCTAAC GCCGTCCAGA GAGCAGCACA GGCCCCCAGC 
AGCTTCCAGC TCCTTTATGA CCTCAAGCTC CCAGTTGAGG ATAAGATCAG GATCATCGCA 
CAGAAAATCT ACGGGGCAGA CGACATTGAA TTGCTCCCCG AAGCCCAGCA AAAAGCCGAA 
GTCTACACGA AGCAGGGCTT TGGGGATCTA CCCATCTGCA TGGCGAAAAC ACACTTGTCC 
CTGTCTCATA ACCCAGAGCT AAAAGGTGTG CCGAAAGGCT TTGTGCTGCC CATCCGCGAC 
ATCCGCGCCA GCGTGGGGGC TGGTTTTCTG TACCCCTTAG TAGGAACGAT GAGCACGATG 
CCTGGACTCC CCACCCGGCC CTGTTTCTAT GATATTGATT TGGACCCTGA AACCAAGCAG 
GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010586988.2
CDS	682..3759
Translation	MIPASLSSPTSFTLPPPSARRITGPLQYFVSNPSSLQTAHAQSRTSERMRSSVIPGWALSTWARLSLLATEGRRTSLLLVSIARSGLVRAMAPAGILNGKVVSAQIRERLKNQVTQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAEEIGIKATHTKLPRTATESEVLKCIISLNEDSTVHGLLVQLPLDSENPINTETVINTIVPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVSKGDILVVATGRPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYNEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGEWVIQYNSLNLKTPVPSDIDISRAYKPKPIGNLAREIGLLSEEVELYGETKAKVLLSALERLRHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAYLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGYTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASNKKGEPISAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYAEENLELVEKGFSNLKKQIENAKLFGVPVVVAVNVFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALANAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQQKAEVYTKQGFGDLPICMAKTHLSLSHNPELKGVPKGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETKQVNGLF

Target ORF information:

RefSeq Version	XM_010588686.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010588686.2

ORF Insert Sequence:

ATGATTCCCG CCAGCCTATC ATCACCCACT TCCTTCACAT TGCCTCCTCC TTCGGCCCGC 
CGTATCACAG GCCCCCTACA GTATTTTGTT AGTAACCCTT CGTCCCTTCA GACTGCGCAC 
GCTCAGTCAC GGACGTCGGA GCGCATGCGC AGTAGCGTGA TCCCCGGGTG GGCGCTTAGC 
ACGTGGGCTA GGCTGTCCCT TTTGGCCACA GAAGGACGTA GGACCTCGCT TTTGTTGGTG 
TCCATCGCGC GCAGCGGACT AGTAAGGGCC ATGGCGCCGG CAGGAATCCT GAACGGGAAG 
GTGGTCTCCG CGCAAATAAG GGAGAGACTG AAGAACCAAG TCACTCAGAT GAAGGAGCAA 
GTACCTGGGT TCACACCAGG CCTAGCAATT TTGCAGGTTG GCAACAGAGA TGATTCAAAT 
CTTTATATAA ATGTGAAGCT GAAGGCTGCT GAAGAGATTG GGATAAAAGC CACTCACACT 
AAGTTACCAA GAACAGCCAC AGAATCCGAG GTGTTAAAGT GCATCATATC TTTGAATGAA 
GACTCCACTG TGCATGGATT GCTAGTGCAG CTCCCTTTAG ACTCAGAGAA TCCCATTAAC 
ACTGAAACAG TGATCAATAC CATTGTACCC GAAAAGGACG TGGATGGATT GACTAGCATC 
AGTGCTGGAA AACTTGCTAG AGGTGACCTA AATGACTGTT TCATTCCTTG TACACCTAAA 
GGATGCTTGG AACTCATCAA AGAGACAGGG GTGCAGATTG CCGGAAGGCA TGCTGTGGTT 
GTTGGGCGCA GTAAAATAGT TGGTGCCCCG ATGCATGACT TGCTTCTGTG GAACAATGCC 
ACAGTGACCA CTTGCCACTC AAAGACTGCC AATCTGGATG AGGAGGTAAG TAAAGGTGAT 
ATCCTGGTGG TTGCAACTGG TCGGCCAGAA ATGGTGAAAG GGGAGTGGAT TAAACCCGGG 
GCAGTCGTCA TCGACTGTGG AATCAATTAT GTTCCAGATG ATACAAAACC GAACGGGAGA 
AAAGTTGTGG GTGACGTGGC ATACAACGAG GCCAAAGAGA GGGCAGGCTT CATCACCCCT 
GTTCCTGGCG GTGTCGGGCC CATGACGGTG GCAATGCTAA TGCAGAGCAC AGTGGAGAGT 
GCAAAACGTT TCCTGGAGAA ATTTAAGCCG GGAGAGTGGG TGATTCAGTA TAACAGCCTT 
AACCTCAAGA CACCTGTCCC TAGTGACATT GATATATCAC GAGCTTACAA ACCAAAGCCC 
ATTGGTAACC TGGCTCGAGA AATTGGTCTG CTCTCTGAAG AGGTGGAATT GTATGGTGAA 
ACCAAGGCCA AGGTTCTGCT TTCAGCGCTA GAACGCCTCA GGCATCAGCC TGATGGAAAA 
TATGTGGTGG TGACGGGAAT AACTCCAACA CCCCTGGGAG AAGGGAAAAG CACGACGACA 
ATTGGGCTGG TACAAGCCCT TGGTGCCTAC CTTCATCAGA ATGTATTTGC GTGTGTACGA 
CAGCCTTCTC AGGGCCCGAC CTTTGGAATA AAAGGTGGCG CTGCAGGAGG TGGCTACTCG 
CAGGTTATTC CCATGGAAGA GTTTAATCTC CACCTCACAG GGGACATCCA TGCCATCACT 
GCAGCTAATA ACCTCGTTGC TGCAGCCATC GATGCCCGGA TGTTCCATGA ACTGACACAG 
ACAGACAAGG CTCTCTTTAA TCGTTTGGTA CCATCAGTAA ATGGAGTGAG AAAGTTCTCT 
GATATTCAAA TTCGAAGGCT ACGGAGACTA GGCATTGAAA AGACCGACCC TACCACACTG 
ACAGATGAAG AGATAAACAG ATTTGCAAGA CTGGATATTG ATCCGGAAAC CATAACTTGG 
CAGAGAGTGT TGGATACCAA TGATAGATTC CTGAGGAAGA TCACAATTGG ACAGGCTCCA 
ACGGAGAAAG GGTACACGCG GACGGCCCAG TTTGATATCT CTGTGGCCAG TGAAATCATG 
GCTGTCCTGG CTCTCACCAC TTCTCTAGAA GACATGAGAG AGAGACTGGG CAAAATGGTG 
GTAGCTTCCA ACAAGAAAGG GGAGCCCATC AGTGCTGAAG ACCTGGGGGT GAGTGGTGCA 
TTGACGGTGC TTATGAAGGA TGCTATCAAG CCCAACCTCA TGCAGACCCT GGAGGGCACT 
CCAGTGTTTG TTCATGCTGG ACCGTTTGCC AACATTGCAC ATGGGAATTC CTCTATCATA 
GCAGACCGGA TTGCACTCAA ACTTGTTGGC CCTGAAGGGT TTGTAGTGAC TGAAGCAGGA 
TTCGGAGCCG ACATTGGAAT GGAAAAGTTT TTTAACATCA AATGCCGATA TTCTGGTCTC 
CGCCCTCACG TTGTGGTGCT TGTTGCCACT GTCAGGGCTC TTAAAATGCA CGGGGGCGGC 
CCCACGGTCA CGGCTGGACT GCCTCTGCCC AAGGCTTACG CAGAGGAGAA CCTGGAGCTG 
GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA CAAATTGAAA ACGCCAAACT CTTCGGAGTC 
CCGGTAGTGG TGGCTGTGAA TGTATTCAAG ACGGACACGG AGGCTGAGCT GGACCTCGTC 
AGCCGCCTTG CCAAAGAACA TGGGGCTTTT GATGCTGTGA AGTGCACTCA CTGGGCAGAA 
GGGGGCAAGG GAGCCTTAGC CCTGGCTAAC GCCGTCCAGA GAGCAGCACA GGCCCCCAGC 
AGCTTCCAGC TCCTTTATGA CCTCAAGCTC CCAGTTGAGG ATAAGATCAG GATCATCGCA 
CAGAAAATCT ACGGGGCAGA CGACATTGAA TTGCTCCCCG AAGCCCAGCA AAAAGCCGAA 
GTCTACACGA AGCAGGGCTT TGGGGATCTA CCCATCTGCA TGGCGAAAAC ACACTTGTCC 
CTGTCTCATA ACCCAGAGCT AAAAGGTGTG CCGAAAGGCT TTGTGCTGCC CATCCGCGAC 
ATCCGCGCCA GCGTGGGGGC TGGTTTTCTG TACCCCTTAG TAGGAACGAT GAGCACGATG 
CCTGGACTCC CCACCCGGCC CTGTTTCTAT GATATTGATT TGGACCCTGA AACCAAGCAG 
GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MTHFD1 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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