AGL cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	100659336
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Loxodonta africana(African savanna elephant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010589500.1	XP_010587802.1	glycogen debranching enzyme
XM_010589500.2	XP_010587802.1	glycogen debranching enzyme
XM_023547411.1	XP_023403179.1	glycogen debranching enzyme

Pathways from BioSystems
Gene Ontology

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLo38586	XM_023547411.1 Latest version!	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLo38586	XM_010589500.2 Latest version!	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OLo38586	XM_010589500.1	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( XM_010589500.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010587802.1 Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLo38586
Clone ID Related Accession (Same CDS sequence)	XM_010589500.1 , XM_010589500.2 , XM_023547411.1
Accession Version	XM_010589500.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573431.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010587802.1
CDS	198..4796
Translation	MEHSRQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLGWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGISRSCYSLADQLELNPDFSRPGKKYTWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSCDVAKGKYKEKGLPALIENDHQMNCIRKIVGEDVFPKIHLWEFFQVDVNKAVEQFRRLLTQEHRKITKSDPVQHLKIIQDPEYRRLGCTVDMDLALATFIPHDKGPAAMDECCNWFRKRIEELNSEKRQLVNYHQEQAVNCILGNVFYERLAGHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHFPDKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSARDSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVRRSAYDALPSSTIVSMASCASGSARGYDELVPHQISVVSEERFYTKWNPGASPSKTDEVNFQSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERDTEPYRKSENSINGMPNITVEIREHIQLSESKIVKQARVAKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLTQFSSHFKAGSFAVDNSDPVLKNPFASIASKLTLAELNQLLYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGRWFQAMFFYLKQIPRYLIPCYFDAILVGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPRLSPSLTDVPYRLNEITEEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGIDILKCPVSRMYPTDDSRPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNVTAGVNDETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKRNIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDRSDPDEKHPDLVHKRGMYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYTKSVFLVKNVLSRHYVHLERSPWKGLPELTNENGRYCPFSCETQAWSIATVLEAIYDL

Target ORF information:

RefSeq Version	XM_010589500.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010589500.1

ORF Insert Sequence:

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_010589500.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010587802.1 Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLo38586
Clone ID Related Accession (Same CDS sequence)	XM_010589500.1 , XM_010589500.2 , XM_023547411.1
Accession Version	XM_010589500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573431.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 16, 2018 this sequence version replaced XM_010589500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010587802.1
CDS	257..4855
Translation	MEHSRQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLGWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGISRSCYSLADQLELNPDFSRPGKKYTWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSCDVAKGKYKEKGLPALIENDHQMNCIRKIVGEDVFPKIHLWEFFQVDVNKAVEQFRRLLTQEHRKITKSDPVQHLKIIQDPEYRRLGCTVDMDLALATFIPHDKGPAAMDECCNWFRKRIEELNSEKRQLVNYHQEQAVNCILGNVFYERLAGHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHFPDKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSARDSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVRRSAYDALPSSTIVSMASCASGSARGYDELVPHQISVVSEERFYTKWNPGASPSKTDEVNFQSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERDTEPYRKSENSINGMPNITVEIREHIQLSESKIVKQARVAKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLTQFSSHFKAGSFAVDNSDPVLKNPFASIASKLTLAELNQLLYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGRWFQAMFFYLKQIPRYLIPCYFDAILVGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPRLSPSLTDVPYRLNEITEEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGIDILKCPVSRMYPTDDSRPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNVTAGVNDETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKRNIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDRSDPDEKHPDLVHKRGMYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYTKSVFLVKNVLSRHYVHLERSPWKGLPELTNENGRYCPFSCETQAWSIATVLEAIYDL

Target ORF information:

RefSeq Version	XM_010589500.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010589500.2

ORF Insert Sequence:

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_023547411.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023403179.1 Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OLo38586
Clone ID Related Accession (Same CDS sequence)	XM_010589500.1 , XM_010589500.2 , XM_023547411.1
Accession Version	XM_023547411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573431.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023403179.1
CDS	289..4887
Translation	MEHSRQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLGWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGISRSCYSLADQLELNPDFSRPGKKYTWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSCDVAKGKYKEKGLPALIENDHQMNCIRKIVGEDVFPKIHLWEFFQVDVNKAVEQFRRLLTQEHRKITKSDPVQHLKIIQDPEYRRLGCTVDMDLALATFIPHDKGPAAMDECCNWFRKRIEELNSEKRQLVNYHQEQAVNCILGNVFYERLAGHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHFPDKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSARDSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVRRSAYDALPSSTIVSMASCASGSARGYDELVPHQISVVSEERFYTKWNPGASPSKTDEVNFQSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERDTEPYRKSENSINGMPNITVEIREHIQLSESKIVKQARVAKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLTQFSSHFKAGSFAVDNSDPVLKNPFASIASKLTLAELNQLLYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGRWFQAMFFYLKQIPRYLIPCYFDAILVGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPRLSPSLTDVPYRLNEITEEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGIDILKCPVSRMYPTDDSRPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNVTAGVNDETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKRNIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDRSDPDEKHPDLVHKRGMYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYTKSVFLVKNVLSRHYVHLERSPWKGLPELTNENGRYCPFSCETQAWSIATVLEAIYDL

Target ORF information:

RefSeq Version	XM_023547411.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023547411.1

ORF Insert Sequence:

ATGGAACACA GTAGACAGAT TCGAATTTTA TTTCTAAATG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGGAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTAGGATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AATATCTCGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGGTAAA AAATATACCT GGAATGATGT TGGACAGCTG 
GTGGAAAAAT TGAAAAAGGA ATGGAACATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCACGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCGCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCTC TTTGGCATTT ATCCTGTGAT 
GTTGCAAAAG GGAAATACAA AGAAAAAGGA TTACCTGCGT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AGTTGGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAG 
TTTTTCCAAG TAGATGTTAA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGAAAA TAACTAAGTC TGACCCAGTG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG GACCTTGCAC TAGCAACTTT CATACCACAT 
GACAAAGGGC CAGCTGCAAT GGATGAATGC TGTAATTGGT TCCGTAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCGTCA ACTCGTGAAC TATCATCAGG AACAGGCTGT TAATTGCATT 
TTGGGAAATG TTTTTTATGA ACGACTGGCG GGCCATGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCGT TTGAAGAAAT GACCCTCTCC 
GCAGAAGAAT CTATGATTCA TTTCCCCGAT AAAGCTTGTT TTCTGATGGC GCACAACGGC 
TGGGTCATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA AGTATATCTA 
AGGAGAGAAC TTATATGTTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAGCTGC GACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ATGTAGCTGA GTACATGTTG 
GACGCTGCCA GGAAATTGCA ACCCAATTTG TATGTGGTAG CCGAACTGTT CACAGGAAGC 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAC GTGATAGCCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAGCCC 
GTTGGATCGT TTGTTCAACC CTGTTTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTC 
TTTATGGACA TTACCCATGA CAATGAGTGT CCTATTGTGC GTAGATCAGC GTATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCA TCTTGTGCTA GTGGAAGTGC TAGAGGCTAC 
GATGAATTAG TGCCGCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATCTCCATC CAAGACAGAT GAAGTTAATT TCCAAAGTGG CATTATCGCA 
GCCAGATGCG CTATCAATAG ACTTCACCAA GAGCTTGGGG CCAAGGGTTT TATTCAGGTA 
TACGTGGATC AGGTTGATGA AGACATAGTG GCGGTTACAA GACACTCACC TAGTATCCAT 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC GTTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAGACACGGA ACCTTATAGG AAGAGTGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTCAGTG AAAGTAAAAT TGTTAAACAA 
GCTAGAGTTG CCAAAAAGGG ACCCAATGAA TATATTCAAG AGATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGATC CGCACGCACA GGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAACT CAGATCCTGT ATTAAAAAAT CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGTT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATA ATATACCAAA CTGGTCATCT CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGATTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAGATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTCATCC CATGTTACTT CGATGCTATA TTGGTTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAACGGGT CAACATTCGT GAAACACCTC 
TCGCTAGGTT CAGTTCAGTT GTGTGGCGTT GGAAAATTCC CTTCTTTACC ACGTCTGTCA 
CCTTCACTTA CGGACGTACC ATATAGACTA AATGAGATCA CAGAAGAGAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCGGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TTTAGAGGCC 
AGGAACATTA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTCCTT 
GGTGAAGGAA CTCATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGACT ACTGTAAAAT GGTTCCAAAT GGTATAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCCCGTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTA 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CAGAGAAAGA 
AATGCTGGTC CACAGATCGA TCGAAACATG AAGGACGAAG GTTTTAATGT AACTGCAGGG 
GTTAATGATG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TGCGTTGGTT GCTGGAATTA 
TCGAAAAGAA ATATTTTTCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ATCGTTCTGA CCCTGATGAA AAGCATCCTG ATTTGGTTCA CAAACGTGGC 
ATGTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCTGAT 
GATATGGTTT ACTGTGGAGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CTATTGGATA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCGGAGAGTT ACACAAAGAG CGTATTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAGCTGA CCAATGAGAA TGGCCGATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTGTTCTTGA GGCAATTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AGL cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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