LOC106461035 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106461035
Entrez Gene ID	106461035
Full Name	uncharacterized LOC106461035
Gene Type	protein-coding
Organism	Limulus polyphemus(Atlantic horseshoe crab)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022387856.1	XP_022243564.1	uncharacterized protein LOC106461035
XM_013920817.1	XP_013776271.1	uncharacterized protein LOC106461035

Pathways from BioSystems
Gene Ontology

The following LOC106461035 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106461035 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLi06487	XM_013920817.1 Latest version!	Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OLi111078	XM_022387856.1 Latest version!	Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106461035 ( XM_013920817.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_013776271.1 Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.

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CloneID	OLi06487
Clone ID Related Accession (Same CDS sequence)	XM_013920817.1
Accession Version	XM_013920817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441641600000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	uncharacterized protein LOC106461035
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGTGACAG AAGTTACAAA CCCAAATAGT TTTTATTGCC AGCTTCAGAA TGCAGTAGAA 
CAGTTGGAGA CATTGACTGT TGACCTTGAA ATGTATTATG GGAATCTTGG TCCAAAGGAT 
GAAGTTTTGA CACAACCTAG AGAGGATATT CCATGTGTAG CTCAGTTTAG TGAAGATGGT 
GCTTGGTATC GAGCTTTGAT CACTAACATT TCAACATCAG GGGAGTATGA GGTCTTATTT 
GTGGATTATG GCAATTCTGA ATGTTGTCAT CAAGATGTTG TAAAATGTAT TAAGCCTCAG 
TTTTTAAATC TTCCCAAACA AGCAGTTTGG TGTACACTTG CTGGTGTGCC TCATCTGGTA 
TGGTCAACTG AAAACAGAGA AAAATTTGAA ACTATGGTAT TAGAAAAAAC TCTCTCAGCT 
GTGTTTAAAG GTACTGGACC AGGAAAAAGT CAGTATCAAG TGGAGTTGAT AGATGGAAGC 
AACAACAGGA TAAACACCAT TTATCAATCT ACTGTCAAGG GTAAAACCTC ATTTTCATCT 
ATTCCTTCAA AAGTACCACC CAAACCTTCT GTAGGTTTTG AAGAAGATGC TTGTGAAGAA 
AATCCCTCTA ATGGACTTCA ACCAGTTGCC AACAGCTTCT CAAGACCTAC CAGAGATCTG 
AGTAGGTGGG ATAATAATCC ACAAAGAAAT ATGTATAATG AAGCATTTGT TAATAAGGGG 
AAACAATTTG GGAAAGCGAC TTCGCCTGGA AGTTCTTCAA ATGAAAATGA ATCACTATCC 
AGAAAAGACT TTGGTAGAAA GCAGTCCGGT ATTCAGCACT TGTCACATAC TCCTGACAAA 
AGTCAGTGGA GAGATCACCC CTCAAAATTT GGTAAAGCTG ATTGTAATCG TTCTCGAATA 
TCTAGTGGAG ATTCCAGATC TTCAGATGAG TCTAGTGGTA GCTTGAAGAG AAATATGAAA 
CATCCAAGAG GATCTTTCAC AGATGAAGCC CGATCTACAA AACGCTCTAT GTCAAACCAA 
ATTCAAGATT TAATTTCAAG GTCAAAAAAT CAAAACACTT CTTTACAAAG GGAAAATGTT 
GAAGTTAAAA TTTCCACCAA TTTTAAAGAA CAAGTAATAC CAGACAAAGC TTTTAGGGAT 
GTGACTGTTG TCTATGCAAT TAGTCCAAGT GAATTTTACT GTCAGTTGAC TGAACATGCT 
TCTGAACTCA TAGAATTAAT GAAGAAGTTG GAAGAAACGT ACAGTGAATT GAATGACAGC 
AGTTTGTCAT TGATTAATCC ACATGTTGGA AGTCCATGTG CAGCTTTTTA TTCAGCAGGT 
GGTGCTTGGT ATCGAGGGAA AGTTGTTGCC ATTCTAGACA CTTTTGTTGA GGTACAGTTT 
GTCGATTATG GAAATTCTGA GAAAGTTCCT ACCAATAAAG TTAAGAGTTT AAAATCTGAA 
TTTTTCTATT TGCCAATACA AGCAATTAAG TGCAGCTTGT ACAACACTCT TCCTCGGGAA 
TCATCATGGA CCATTGATGA TATCAATAAG TTTTCTGAAA TGACAAGTGA AAAATCTTTA 
GTGGGACAGT TTGTTCGCAA ACAGGTCAGT GGTAGTTTCT TAATTAATTT GATTGATATG 
GATAAATTAG AGAATGATAT TTTAAACAAG GATTTTGTTG AGTCTGGACA TGGAAAAACA 
CTTGGAGTGA AAACACCAAT TATAAGATCT CTGTCTTCTG CTATTGCAGA TGTGGAAATT 
GCAGATCCTT CAATAAGGTT AGGTGAAAAG GAACTTGTTC AGGTGACATG GGTTATGAAT 
CCTGGAGAGT TCTACTCCCA ACTTCAGTCA AGTAGTCAAC AATTTAACTA TTTAATGTGT 
GAAATTCAGG CTTTTTATGG GGCTTCAACT TCAGCGGCTA CATCTATTGA TCAGCCAAAG 
GTAAACCAAG TGTGTGCTGC AAGATTTACA GAAGATGATG CTTGGTATCG TGCAAGAATC 
CTTTATCTGA AAGATGGTAT GATTGGTGTT CTTTATGTTG ATTATGGAAA TGGCCAAGAA 
ATACCACGAG ATAGGATCAA GTACTTGATT CCAGATTTTG CAGCTTTACT TCCTTCCCAA 
GCTGTGAAGT GTTACTTAAA AGGTATCAAA CCACCTAATG ATAATTGGCC TTCTGTTGAT 
AAAAGCACGT ATGATAGTTT CTTTGAAGGA AAAACAGAAT GCACATTTAT TAAAAAGATA 
GATAGTGGAT TTTTAGTGGA CATGGAACAG AATGGAAAAA ACCTAGCTGA GGAGTTTATT 
TTAAAAAATT TAGCAGAAAG AGAAACTGCA TTAATTCCAG AACAGTCAGA ATTTAAAACA 
TGTGCCTATC TACAATCACC AAGGTTTGAA AACAAAACAA TTAAATTAAA TCAATCTGTA 
GAAGTTGTTG TATCTTTTGT GGAAAGTCCA CAGTCTTTTT GGTGTCAGTT AAAACAAGCC 
ATTCCTCAGC TTGATTCCTT GATGGAACAG ATAGATTCCT ACTACTCATC ACTCTCGGAT 
GATGAAGATG TTCTCTCTGC TTCAGAGAAG TTTTGTGTGG CAAAGTTTAG TGAAGATGAA 
AGTTGGTACA GAGCTTTTAT TCAGGAACAT GTATCACCAG AAACTGTAAG AGTCTACTAT 
CTTGATTATG GAAACTCAGA AGAGGTGGAA TTTTCTAAGT GTAAAAGCCT GCATGAAAAA 
TTCAAAGGTC TCTCAGCTCA AGCCATTAAG TGTCGACTCT ATGGCATGCC ATCTCAAAGT 
ACTGAACAAC AAAGCAAAGA TTTTGTGGAC ATAATTGATG AGAAAGAAGT GGTGATTAAA 
GTGCGTAAGA AGAAACCTAG CATACTGGCA GTGAATGTTT TGGATAATTC CTCAGGCACA 
GAAGTGGACA TTGGGGAGAC TGTTTATGGG ACCATGGAAG ATGAATGTCC TTTGAAAATC 
AATGGACAGC CAGATATTCC AGAAGGAGAA CAGGAAGGAT ATTTAATCAT AGATAGTTTA 
TATAATCTTG ATTTTGAATT CTTCCATGTG CAACTTAGTA GTGCAAAAAA CCAGATCAAC 
GATATGAGTT CTAGAATCAA AACAAGTGAA CTTTTATTGT CACACGAAGT AGAAATTGGT 
GAAAACTACT GTGTGCGAGA AAATGACATT TGGTATAGAG TCAAAGTGTA TCATAATAAT 
GGTACTGTTA TCTCAGCAAA AATGATAGAC TGTGGGAAAT ATTCATCCTT TGATGCTGAT 
ATATTTTATG AACTACCAGA AGATCTGGAA GCTTTTCCTC CTTTAGCCAT TCCCTGCAAA 
CTTTATGGGC TAGAGTGGAA GCGTTCAAAC AAAGAGTTAG TAGAAAAATG CTTGGCTGGC 
AGTGATTTGC TAAATGAGAA GAAATTCACC ATCAAATTCA TTAATAACTG GGAACAAGGA 
GCATTTGTTT CTATAATGGA TGGAGAAGTG GATATTAGTC AATCACTTGT CACTAAAGGT 
TTGGCAATGG CTATGTTTAA AGAAGATGAA GCTATGATTA CAGCTGGTTA TACTCTAACA 
ACCTTGAATA ATGACCCTTT GCTTGTCACT TTAAGTCATT TTGAGTCTAT AAACATGTTT 
TATGTGACAC TTGTAAAGGA GCTTAAAAAT CTTGATATCT TATCGGCAAA ACTTCAAGAT 
ACGTTTGAAG CTATGGACGA GGAAGAAGGT CAGGTGACAG AACCATGTTG GGGCAAAGCA 
TGTGTATGCA AGTTTAATGA AGATGGTGTG TGGTACAGAG GAGTAATTAC ATCAGTTGAT 
AAAGAAGTTG TCACTGTCTA TTTCGTAGAT TTTGGAAATG AGGAAGAAAC TACTATAGAC 
CAACTAAAGG AGTACCCAAA AGATATTTTG AATTCTAACA ACTTTGAGTC AGATTGCTTT 
AATTTTCCAG CCTTTGCTGT ATGTTGTTCC TTGTGTGACA CAACTTCAGT TGAGGGAAAA 
GAACAAAAAA TTGAGGAATT TTTAAAAGAA TTAGTTGGAA AGTATTTGAC TTTAGAGATT 
TGTGAAAGAG GTTCACCCTA CATTGTTTTG CTATCAATGA ATGGAATGGA AGTTAGTGAA 
ACTATGTGTA AGAAAGAACT TGTGAACTTA AACAAGCCAA CAGTTAACTA TACAGAAGGA 
AGTCGATCAC TGTCTGAATG TAAAGATGGA GACTGTAACT GTTTTTTTAT AGATCTAGGA 
ATAACAGATG AAAAAAGTTC TAAAGAATTA AAAAAACTGA AGGTTTCTTT TTCCTGGCAG 
CTTCCTTTTT CTTTGAAATG TTCTTTGTCA CATGCTTTGG CTTGTGCTGA ATTTAATCTA 
AAGCTGTTTA GTGAAAGTGA TAATGTAGAG GCAGTCAGAA AAGAAGTAAT TGGAAAGATG 
GTAGAAGTTG AGTTTATGAG CAGTGAACCA ATCCCAGCAG TGAAACTCCT TGTTGCAAGA 
GATATGTCTG AAACAAATCA AGATTATAAT CAAATAACTG ACCTGATTGA AGAGGTATTA 
AAATCAAAGT TTCTGCTCTC TTTGAAAGAG ATGGAACAAG AAAAGGAACA GAGGTATTCA 
CCACTAACTA CCCCACATCT ACAGAAGTAC CAACCTTTAG ATATACCCTT GCATACCAAG 
TTAGAAGTGA AGGTTAGTTA TGTAAATACT CCTAGTGAGT TCTGGGTGCA GCTAGTTTAC 
CAACAAGAAC AACTTCACAA CATAATGGAT GAAATAGCAG ACATATATTC AAATATCTTT 
GAAGATGAAT TTGTTATTAC AGAACCTCAA CCTGGGACTC CTTGTGTTTC TATTTTCTCA 
GAAGATGGTG GATGGTACCG AGGATATGTC AAGGAAGTAA TGGGTGGATC TGTAAAAATC 
CATTTTGTGG ATTATGGAAA TAGTGAAGAA GTTCCTCTTA CTTTTGTGAG GGAGATCTCT 
TCAGATCTGT TGAAAACCCC CCAGCTAGCA GTCAAATGTT CTCTCCATGA TGTTAAGCCT 
AACAGTGACT CAGAATGGAA TATTGACCAG TGTGAAGTTT TCCAGGAAAT AGTAAATGAA 
GAAAAGGTTT TTTTAGCAGA ATTTTGCAGC AGTATTGATA ATACATACCA AGTTAAACTG 
TTGGACATGG GAATTGATGT AGGAAAGACC TTCTTGTCAA AATGCATTGC TTCTAGCTCA 
TCAGTACAAG AAGAAAAGTC GGATATTGTT AAGCTGACAC ATATTTTGCT TCAAGAACCT 
GTACATGAAG CAGAATGTAT AAGTCCTAAT AAAGTTAAGT CAAATGAAAT CCTTAGTTTC 
AATCAAATGG AGAATGGAAC CACCAGTTTG TTTTCTAAAG AAAATGATTC CTTCATTCTT 
AAAGAGATGG AAAATGAATC ATTCAGTGTT GTAAGTAACA GAAATGTGTC ATTCAGCATT 
ACAGATAATG AAGAAGAATC ATTTAGTGTT ACAAATAATA CTGATTCATC AAACTTTATG 
GGTATTGGAA ATGAGCAGTT CAGCATTATA CAAAAAGAAG ATTCATTTTA TGATGTAAAC 
CAAGAATTTG AATCATGTAA TATTATGGAC AAGGACGAAG ATTCATCCAG TGTTATAAAT 
GAAGAAAAAC CAAACAGTGA CATGAACAAG CAATATAGAT CAGACAGTGA CATGTATCAG 
TATGATCCAA CTAATGTTAT AATGAAAAAT GAAATTTGTA ACATTGTTAA GGAAGATACT 
GGTGAGCCAT CTGATTTTGG TAATGAATCA GAAAATTCTG TCAGTATCTG CACACCAGAG 
GAAGATATTT TAGAAAAAAA TATAAACCCA GAAGACTTTG CATCATTGGG TGAACCAAAT 
CCTCTTTCCC AGCAAGGGGG TAATCAACAG GACCAGTTTG TCCCAGCAGT AATCTCCATG 
AAGAAGTTAG ATGAAGAAAC TACAGCAAGG ACTGCTTATG AGATTGAACC TAATCTAGTT 
TCTGTTCAGT CTAAAGAAAT TCTGGAAGAT TGTACATTGT TAGGAGAAAG TTTTAAAAAT 
GAGTTTGTTC TTTCCAATAA AGACATGACT GAGGTAAATC AAAGGTTTTT ATCTAATGTG 
GATAGAAACT TGAGTGATCA TGACAAGATA ACAGAGTCAT CAGATGATTA TTTGGAATAC 
AAAAGTAATA TAGAAGCAAA GAGTGGGGAG GAGTTAGAAT ATTGTACACA TGAAGAGAAG 
TTAAGCCCAT GGGAAGGGGA AAATGTACCA GCATCTACAA AATTAGATGA AACAATGCCA 
CATGAACAAG TATCATTTTG TAACAGAATT AGTAAAGAAG ATAAAGATGT GGAAAATGAG 
TGTGAACAAG AATTATCAAC AAATAGAGGA CAACAGTCAG AACAGTTAAT AATAGCTTGT 
ACTGAAAAAG CATTAACTTC TAACGACCAA GAAGAACAGC CCTTACTAAA AAAGGAATCA 
GCTAAAGAAA TTGTTTGTAG TGAACACACA ACACTAGATG GAGAGGAATC AGATAAAGAA 
ATTGTTTACA GTGAACACAC AACACTAGAA GGAGAGGAAT CAGATAAAGA AATTGTTTAC 
AGTGAACACA CAACACTAGA AGGAGAGGAA TCAGATAAAA AAATTGTTTA CAGTGAACAC 
ACAACACTAG AAGGAAAGGA AATAGATAAA GAAAATGTTT ACAGTGAACA TACGACACTA 
GAAGGAAAGG AATCAGATAA AGAAATTATT TGCAGTGAAC ATACGACACT AGAAGGAAAG 
GAATCAGATA AAGAAATTAT TTGCAGTGAA CACACAACAC TAGAAGAAAA GGAATTGGAT 
AAAGAAATTG TTTGCAGTGA ACACATAACA CTAGAAGAAA AGGAATTGGA TAAAGAAATT 
GTTTGCAGTG AACACATAAC ACTAGAAGGA GAGGTATCAG ATAAAGAAAT TGTTTGCAGT 
GAACACACAA CACTAGAAGA AGAGAAATCA GATAAAGAAA TTTACAGTGA ACACACAACA 
CTAGAAGGAG AGGAATCAGA TAAAGAAATT GTTTACAATG AACACACAAC ACCAGAAGGA 
AAGGTATCAG ATAAAGAAAT TGTTTGCAGT GAACACATAA CACTAGAAGG AAAAGACCTG 
GATAACCTAA AAGACATTGC TAGAGGTGAT AATCCAGAAG ATGATGATGA TGATTTAGGT 
ACAACCACTA ATAAGCCTTC AGAAATCTCC ACACCCGATT CACATGGCAA AACCTCATTT 
TCAACAGGCG CAGAAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013776271.1
CDS	12..7232
Translation	MVTEVTNPNSFYCQLQNAVEQLETLTVDLEMYYGNLGPKDEVLTQPREDIPCVAQFSEDGAWYRALITNISTSGEYEVLFVDYGNSECCHQDVVKCIKPQFLNLPKQAVWCTLAGVPHLVWSTENREKFETMVLEKTLSAVFKGTGPGKSQYQVELIDGSNNRINTIYQSTVKGKTSFSSIPSKVPPKPSVGFEEDACEENPSNGLQPVANSFSRPTRDLSRWDNNPQRNMYNEAFVNKGKQFGKATSPGSSSNENESLSRKDFGRKQSGIQHLSHTPDKSQWRDHPSKFGKADCNRSRISSGDSRSSDESSGSLKRNMKHPRGSFTDEARSTKRSMSNQIQDLISRSKNQNTSLQRENVEVKISTNFKEQVIPDKAFRDVTVVYAISPSEFYCQLTEHASELIELMKKLEETYSELNDSSLSLINPHVGSPCAAFYSAGGAWYRGKVVAILDTFVEVQFVDYGNSEKVPTNKVKSLKSEFFYLPIQAIKCSLYNTLPRESSWTIDDINKFSEMTSEKSLVGQFVRKQVSGSFLINLIDMDKLENDILNKDFVESGHGKTLGVKTPIIRSLSSAIADVEIADPSIRLGEKELVQVTWVMNPGEFYSQLQSSSQQFNYLMCEIQAFYGASTSAATSIDQPKVNQVCAARFTEDDAWYRARILYLKDGMIGVLYVDYGNGQEIPRDRIKYLIPDFAALLPSQAVKCYLKGIKPPNDNWPSVDKSTYDSFFEGKTECTFIKKIDSGFLVDMEQNGKNLAEEFILKNLAERETALIPEQSEFKTCAYLQSPRFENKTIKLNQSVEVVVSFVESPQSFWCQLKQAIPQLDSLMEQIDSYYSSLSDDEDVLSASEKFCVAKFSEDESWYRAFIQEHVSPETVRVYYLDYGNSEEVEFSKCKSLHEKFKGLSAQAIKCRLYGMPSQSTEQQSKDFVDIIDEKEVVIKVRKKKPSILAVNVLDNSSGTEVDIGETVYGTMEDECPLKINGQPDIPEGEQEGYLIIDSLYNLDFEFFHVQLSSAKNQINDMSSRIKTSELLLSHEVEIGENYCVRENDIWYRVKVYHNNGTVISAKMIDCGKYSSFDADIFYELPEDLEAFPPLAIPCKLYGLEWKRSNKELVEKCLAGSDLLNEKKFTIKFINNWEQGAFVSIMDGEVDISQSLVTKGLAMAMFKEDEAMITAGYTLTTLNNDPLLVTLSHFESINMFYVTLVKELKNLDILSAKLQDTFEAMDEEEGQVTEPCWGKACVCKFNEDGVWYRGVITSVDKEVVTVYFVDFGNEEETTIDQLKEYPKDILNSNNFESDCFNFPAFAVCCSLCDTTSVEGKEQKIEEFLKELVGKYLTLEICERGSPYIVLLSMNGMEVSETMCKKELVNLNKPTVNYTEGSRSLSECKDGDCNCFFIDLGITDEKSSKELKKLKVSFSWQLPFSLKCSLSHALACAEFNLKLFSESDNVEAVRKEVIGKMVEVEFMSSEPIPAVKLLVARDMSETNQDYNQITDLIEEVLKSKFLLSLKEMEQEKEQRYSPLTTPHLQKYQPLDIPLHTKLEVKVSYVNTPSEFWVQLVYQQEQLHNIMDEIADIYSNIFEDEFVITEPQPGTPCVSIFSEDGGWYRGYVKEVMGGSVKIHFVDYGNSEEVPLTFVREISSDLLKTPQLAVKCSLHDVKPNSDSEWNIDQCEVFQEIVNEEKVFLAEFCSSIDNTYQVKLLDMGIDVGKTFLSKCIASSSSVQEEKSDIVKLTHILLQEPVHEAECISPNKVKSNEILSFNQMENGTTSLFSKENDSFILKEMENESFSVVSNRNVSFSITDNEEESFSVTNNTDSSNFMGIGNEQFSIIQKEDSFYDVNQEFESCNIMDKDEDSSSVINEEKPNSDMNKQYRSDSDMYQYDPTNVIMKNEICNIVKEDTGEPSDFGNESENSVSICTPEEDILEKNINPEDFASLGEPNPLSQQGGNQQDQFVPAVISMKKLDEETTARTAYEIEPNLVSVQSKEILEDCTLLGESFKNEFVLSNKDMTEVNQRFLSNVDRNLSDHDKITESSDDYLEYKSNIEAKSGEELEYCTHEEKLSPWEGENVPASTKLDETMPHEQVSFCNRISKEDKDVENECEQELSTNRGQQSEQLIIACTEKALTSNDQEEQPLLKKESAKEIVCSEHTTLDGEESDKEIVYSEHTTLEGEESDKEIVYSEHTTLEGEESDKKIVYSEHTTLEGKEIDKENVYSEHTTLEGKESDKEIICSEHTTLEGKESDKEIICSEHTTLEEKELDKEIVCSEHITLEEKELDKEIVCSEHITLEGEVSDKEIVCSEHTTLEEEKSDKEIYSEHTTLEGEESDKEIVYNEHTTPEGKVSDKEIVCSEHITLEGKDLDNLKDIARGDNPEDDDDDLGTTTNKPSEISTPDSHGKTSFSTGAEE

Target ORF information:

RefSeq Version	XM_013920817.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013920817.1

ORF Insert Sequence:

ATGGTGACAG AAGTTACAAA CCCAAATAGT TTTTATTGCC AGCTTCAGAA TGCAGTAGAA 
CAGTTGGAGA CATTGACTGT TGACCTTGAA ATGTATTATG GGAATCTTGG TCCAAAGGAT 
GAAGTTTTGA CACAACCTAG AGAGGATATT CCATGTGTAG CTCAGTTTAG TGAAGATGGT 
GCTTGGTATC GAGCTTTGAT CACTAACATT TCAACATCAG GGGAGTATGA GGTCTTATTT 
GTGGATTATG GCAATTCTGA ATGTTGTCAT CAAGATGTTG TAAAATGTAT TAAGCCTCAG 
TTTTTAAATC TTCCCAAACA AGCAGTTTGG TGTACACTTG CTGGTGTGCC TCATCTGGTA 
TGGTCAACTG AAAACAGAGA AAAATTTGAA ACTATGGTAT TAGAAAAAAC TCTCTCAGCT 
GTGTTTAAAG GTACTGGACC AGGAAAAAGT CAGTATCAAG TGGAGTTGAT AGATGGAAGC 
AACAACAGGA TAAACACCAT TTATCAATCT ACTGTCAAGG GTAAAACCTC ATTTTCATCT 
ATTCCTTCAA AAGTACCACC CAAACCTTCT GTAGGTTTTG AAGAAGATGC TTGTGAAGAA 
AATCCCTCTA ATGGACTTCA ACCAGTTGCC AACAGCTTCT CAAGACCTAC CAGAGATCTG 
AGTAGGTGGG ATAATAATCC ACAAAGAAAT ATGTATAATG AAGCATTTGT TAATAAGGGG 
AAACAATTTG GGAAAGCGAC TTCGCCTGGA AGTTCTTCAA ATGAAAATGA ATCACTATCC 
AGAAAAGACT TTGGTAGAAA GCAGTCCGGT ATTCAGCACT TGTCACATAC TCCTGACAAA 
AGTCAGTGGA GAGATCACCC CTCAAAATTT GGTAAAGCTG ATTGTAATCG TTCTCGAATA 
TCTAGTGGAG ATTCCAGATC TTCAGATGAG TCTAGTGGTA GCTTGAAGAG AAATATGAAA 
CATCCAAGAG GATCTTTCAC AGATGAAGCC CGATCTACAA AACGCTCTAT GTCAAACCAA 
ATTCAAGATT TAATTTCAAG GTCAAAAAAT CAAAACACTT CTTTACAAAG GGAAAATGTT 
GAAGTTAAAA TTTCCACCAA TTTTAAAGAA CAAGTAATAC CAGACAAAGC TTTTAGGGAT 
GTGACTGTTG TCTATGCAAT TAGTCCAAGT GAATTTTACT GTCAGTTGAC TGAACATGCT 
TCTGAACTCA TAGAATTAAT GAAGAAGTTG GAAGAAACGT ACAGTGAATT GAATGACAGC 
AGTTTGTCAT TGATTAATCC ACATGTTGGA AGTCCATGTG CAGCTTTTTA TTCAGCAGGT 
GGTGCTTGGT ATCGAGGGAA AGTTGTTGCC ATTCTAGACA CTTTTGTTGA GGTACAGTTT 
GTCGATTATG GAAATTCTGA GAAAGTTCCT ACCAATAAAG TTAAGAGTTT AAAATCTGAA 
TTTTTCTATT TGCCAATACA AGCAATTAAG TGCAGCTTGT ACAACACTCT TCCTCGGGAA 
TCATCATGGA CCATTGATGA TATCAATAAG TTTTCTGAAA TGACAAGTGA AAAATCTTTA 
GTGGGACAGT TTGTTCGCAA ACAGGTCAGT GGTAGTTTCT TAATTAATTT GATTGATATG 
GATAAATTAG AGAATGATAT TTTAAACAAG GATTTTGTTG AGTCTGGACA TGGAAAAACA 
CTTGGAGTGA AAACACCAAT TATAAGATCT CTGTCTTCTG CTATTGCAGA TGTGGAAATT 
GCAGATCCTT CAATAAGGTT AGGTGAAAAG GAACTTGTTC AGGTGACATG GGTTATGAAT 
CCTGGAGAGT TCTACTCCCA ACTTCAGTCA AGTAGTCAAC AATTTAACTA TTTAATGTGT 
GAAATTCAGG CTTTTTATGG GGCTTCAACT TCAGCGGCTA CATCTATTGA TCAGCCAAAG 
GTAAACCAAG TGTGTGCTGC AAGATTTACA GAAGATGATG CTTGGTATCG TGCAAGAATC 
CTTTATCTGA AAGATGGTAT GATTGGTGTT CTTTATGTTG ATTATGGAAA TGGCCAAGAA 
ATACCACGAG ATAGGATCAA GTACTTGATT CCAGATTTTG CAGCTTTACT TCCTTCCCAA 
GCTGTGAAGT GTTACTTAAA AGGTATCAAA CCACCTAATG ATAATTGGCC TTCTGTTGAT 
AAAAGCACGT ATGATAGTTT CTTTGAAGGA AAAACAGAAT GCACATTTAT TAAAAAGATA 
GATAGTGGAT TTTTAGTGGA CATGGAACAG AATGGAAAAA ACCTAGCTGA GGAGTTTATT 
TTAAAAAATT TAGCAGAAAG AGAAACTGCA TTAATTCCAG AACAGTCAGA ATTTAAAACA 
TGTGCCTATC TACAATCACC AAGGTTTGAA AACAAAACAA TTAAATTAAA TCAATCTGTA 
GAAGTTGTTG TATCTTTTGT GGAAAGTCCA CAGTCTTTTT GGTGTCAGTT AAAACAAGCC 
ATTCCTCAGC TTGATTCCTT GATGGAACAG ATAGATTCCT ACTACTCATC ACTCTCGGAT 
GATGAAGATG TTCTCTCTGC TTCAGAGAAG TTTTGTGTGG CAAAGTTTAG TGAAGATGAA 
AGTTGGTACA GAGCTTTTAT TCAGGAACAT GTATCACCAG AAACTGTAAG AGTCTACTAT 
CTTGATTATG GAAACTCAGA AGAGGTGGAA TTTTCTAAGT GTAAAAGCCT GCATGAAAAA 
TTCAAAGGTC TCTCAGCTCA AGCCATTAAG TGTCGACTCT ATGGCATGCC ATCTCAAAGT 
ACTGAACAAC AAAGCAAAGA TTTTGTGGAC ATAATTGATG AGAAAGAAGT GGTGATTAAA 
GTGCGTAAGA AGAAACCTAG CATACTGGCA GTGAATGTTT TGGATAATTC CTCAGGCACA 
GAAGTGGACA TTGGGGAGAC TGTTTATGGG ACCATGGAAG ATGAATGTCC TTTGAAAATC 
AATGGACAGC CAGATATTCC AGAAGGAGAA CAGGAAGGAT ATTTAATCAT AGATAGTTTA 
TATAATCTTG ATTTTGAATT CTTCCATGTG CAACTTAGTA GTGCAAAAAA CCAGATCAAC 
GATATGAGTT CTAGAATCAA AACAAGTGAA CTTTTATTGT CACACGAAGT AGAAATTGGT 
GAAAACTACT GTGTGCGAGA AAATGACATT TGGTATAGAG TCAAAGTGTA TCATAATAAT 
GGTACTGTTA TCTCAGCAAA AATGATAGAC TGTGGGAAAT ATTCATCCTT TGATGCTGAT 
ATATTTTATG AACTACCAGA AGATCTGGAA GCTTTTCCTC CTTTAGCCAT TCCCTGCAAA 
CTTTATGGGC TAGAGTGGAA GCGTTCAAAC AAAGAGTTAG TAGAAAAATG CTTGGCTGGC 
AGTGATTTGC TAAATGAGAA GAAATTCACC ATCAAATTCA TTAATAACTG GGAACAAGGA 
GCATTTGTTT CTATAATGGA TGGAGAAGTG GATATTAGTC AATCACTTGT CACTAAAGGT 
TTGGCAATGG CTATGTTTAA AGAAGATGAA GCTATGATTA CAGCTGGTTA TACTCTAACA 
ACCTTGAATA ATGACCCTTT GCTTGTCACT TTAAGTCATT TTGAGTCTAT AAACATGTTT 
TATGTGACAC TTGTAAAGGA GCTTAAAAAT CTTGATATCT TATCGGCAAA ACTTCAAGAT 
ACGTTTGAAG CTATGGACGA GGAAGAAGGT CAGGTGACAG AACCATGTTG GGGCAAAGCA 
TGTGTATGCA AGTTTAATGA AGATGGTGTG TGGTACAGAG GAGTAATTAC ATCAGTTGAT 
AAAGAAGTTG TCACTGTCTA TTTCGTAGAT TTTGGAAATG AGGAAGAAAC TACTATAGAC 
CAACTAAAGG AGTACCCAAA AGATATTTTG AATTCTAACA ACTTTGAGTC AGATTGCTTT 
AATTTTCCAG CCTTTGCTGT ATGTTGTTCC TTGTGTGACA CAACTTCAGT TGAGGGAAAA 
GAACAAAAAA TTGAGGAATT TTTAAAAGAA TTAGTTGGAA AGTATTTGAC TTTAGAGATT 
TGTGAAAGAG GTTCACCCTA CATTGTTTTG CTATCAATGA ATGGAATGGA AGTTAGTGAA 
ACTATGTGTA AGAAAGAACT TGTGAACTTA AACAAGCCAA CAGTTAACTA TACAGAAGGA 
AGTCGATCAC TGTCTGAATG TAAAGATGGA GACTGTAACT GTTTTTTTAT AGATCTAGGA 
ATAACAGATG AAAAAAGTTC TAAAGAATTA AAAAAACTGA AGGTTTCTTT TTCCTGGCAG 
CTTCCTTTTT CTTTGAAATG TTCTTTGTCA CATGCTTTGG CTTGTGCTGA ATTTAATCTA 
AAGCTGTTTA GTGAAAGTGA TAATGTAGAG GCAGTCAGAA AAGAAGTAAT TGGAAAGATG 
GTAGAAGTTG AGTTTATGAG CAGTGAACCA ATCCCAGCAG TGAAACTCCT TGTTGCAAGA 
GATATGTCTG AAACAAATCA AGATTATAAT CAAATAACTG ACCTGATTGA AGAGGTATTA 
AAATCAAAGT TTCTGCTCTC TTTGAAAGAG ATGGAACAAG AAAAGGAACA GAGGTATTCA 
CCACTAACTA CCCCACATCT ACAGAAGTAC CAACCTTTAG ATATACCCTT GCATACCAAG 
TTAGAAGTGA AGGTTAGTTA TGTAAATACT CCTAGTGAGT TCTGGGTGCA GCTAGTTTAC 
CAACAAGAAC AACTTCACAA CATAATGGAT GAAATAGCAG ACATATATTC AAATATCTTT 
GAAGATGAAT TTGTTATTAC AGAACCTCAA CCTGGGACTC CTTGTGTTTC TATTTTCTCA 
GAAGATGGTG GATGGTACCG AGGATATGTC AAGGAAGTAA TGGGTGGATC TGTAAAAATC 
CATTTTGTGG ATTATGGAAA TAGTGAAGAA GTTCCTCTTA CTTTTGTGAG GGAGATCTCT 
TCAGATCTGT TGAAAACCCC CCAGCTAGCA GTCAAATGTT CTCTCCATGA TGTTAAGCCT 
AACAGTGACT CAGAATGGAA TATTGACCAG TGTGAAGTTT TCCAGGAAAT AGTAAATGAA 
GAAAAGGTTT TTTTAGCAGA ATTTTGCAGC AGTATTGATA ATACATACCA AGTTAAACTG 
TTGGACATGG GAATTGATGT AGGAAAGACC TTCTTGTCAA AATGCATTGC TTCTAGCTCA 
TCAGTACAAG AAGAAAAGTC GGATATTGTT AAGCTGACAC ATATTTTGCT TCAAGAACCT 
GTACATGAAG CAGAATGTAT AAGTCCTAAT AAAGTTAAGT CAAATGAAAT CCTTAGTTTC 
AATCAAATGG AGAATGGAAC CACCAGTTTG TTTTCTAAAG AAAATGATTC CTTCATTCTT 
AAAGAGATGG AAAATGAATC ATTCAGTGTT GTAAGTAACA GAAATGTGTC ATTCAGCATT 
ACAGATAATG AAGAAGAATC ATTTAGTGTT ACAAATAATA CTGATTCATC AAACTTTATG 
GGTATTGGAA ATGAGCAGTT CAGCATTATA CAAAAAGAAG ATTCATTTTA TGATGTAAAC 
CAAGAATTTG AATCATGTAA TATTATGGAC AAGGACGAAG ATTCATCCAG TGTTATAAAT 
GAAGAAAAAC CAAACAGTGA CATGAACAAG CAATATAGAT CAGACAGTGA CATGTATCAG 
TATGATCCAA CTAATGTTAT AATGAAAAAT GAAATTTGTA ACATTGTTAA GGAAGATACT 
GGTGAGCCAT CTGATTTTGG TAATGAATCA GAAAATTCTG TCAGTATCTG CACACCAGAG 
GAAGATATTT TAGAAAAAAA TATAAACCCA GAAGACTTTG CATCATTGGG TGAACCAAAT 
CCTCTTTCCC AGCAAGGGGG TAATCAACAG GACCAGTTTG TCCCAGCAGT AATCTCCATG 
AAGAAGTTAG ATGAAGAAAC TACAGCAAGG ACTGCTTATG AGATTGAACC TAATCTAGTT 
TCTGTTCAGT CTAAAGAAAT TCTGGAAGAT TGTACATTGT TAGGAGAAAG TTTTAAAAAT 
GAGTTTGTTC TTTCCAATAA AGACATGACT GAGGTAAATC AAAGGTTTTT ATCTAATGTG 
GATAGAAACT TGAGTGATCA TGACAAGATA ACAGAGTCAT CAGATGATTA TTTGGAATAC 
AAAAGTAATA TAGAAGCAAA GAGTGGGGAG GAGTTAGAAT ATTGTACACA TGAAGAGAAG 
TTAAGCCCAT GGGAAGGGGA AAATGTACCA GCATCTACAA AATTAGATGA AACAATGCCA 
CATGAACAAG TATCATTTTG TAACAGAATT AGTAAAGAAG ATAAAGATGT GGAAAATGAG 
TGTGAACAAG AATTATCAAC AAATAGAGGA CAACAGTCAG AACAGTTAAT AATAGCTTGT 
ACTGAAAAAG CATTAACTTC TAACGACCAA GAAGAACAGC CCTTACTAAA AAAGGAATCA 
GCTAAAGAAA TTGTTTGTAG TGAACACACA ACACTAGATG GAGAGGAATC AGATAAAGAA 
ATTGTTTACA GTGAACACAC AACACTAGAA GGAGAGGAAT CAGATAAAGA AATTGTTTAC 
AGTGAACACA CAACACTAGA AGGAGAGGAA TCAGATAAAA AAATTGTTTA CAGTGAACAC 
ACAACACTAG AAGGAAAGGA AATAGATAAA GAAAATGTTT ACAGTGAACA TACGACACTA 
GAAGGAAAGG AATCAGATAA AGAAATTATT TGCAGTGAAC ATACGACACT AGAAGGAAAG 
GAATCAGATA AAGAAATTAT TTGCAGTGAA CACACAACAC TAGAAGAAAA GGAATTGGAT 
AAAGAAATTG TTTGCAGTGA ACACATAACA CTAGAAGAAA AGGAATTGGA TAAAGAAATT 
GTTTGCAGTG AACACATAAC ACTAGAAGGA GAGGTATCAG ATAAAGAAAT TGTTTGCAGT 
GAACACACAA CACTAGAAGA AGAGAAATCA GATAAAGAAA TTTACAGTGA ACACACAACA 
CTAGAAGGAG AGGAATCAGA TAAAGAAATT GTTTACAATG AACACACAAC ACCAGAAGGA 
AAGGTATCAG ATAAAGAAAT TGTTTGCAGT GAACACATAA CACTAGAAGG AAAAGACCTG 
GATAACCTAA AAGACATTGC TAGAGGTGAT AATCCAGAAG ATGATGATGA TGATTTAGGT 
ACAACCACTA ATAAGCCTTC AGAAATCTCC ACACCCGATT CACATGGCAA AACCTCATTT 
TCAACAGGCG CAGAAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106461035 ( XM_022387856.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022243564.1 Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.

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CloneID	OLi111078
Clone ID Related Accession (Same CDS sequence)	XM_022387856.1
Accession Version	XM_022387856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	uncharacterized protein LOC106461035
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGTGACAG AAGTTACAAA CCCAAATAGT TTTTATTGCC AGCTTCAGAA TGCAGTAGAA 
CAGTTGGAGA CATTGACTGT TGACCTTGAA ATGTATTATG GGAATCTTGG TCCAAAGGAT 
GAAGTTTTGA CACAACCTAG AGAGGATATT CCATGTGTAG CTCAGTTTAG TGAAGATGGT 
GCTTGGTATC GAGCTTTGAT CACTAACATT TCAACATCAG GGGAGTATGA GGTCTTATTT 
GTGGATTATG GCAATTCTGA ATGTTGTCAT CAAGATGTTG TAAAATGTAT TAAGCCTCAG 
TTTTTAAATC TTCCCAAACA AGCAGTTTGG TGTACACTTG CTGGTGTGCC TCATCTGGTA 
TGGTCAACTG AAAACAGAGA AAAATTTGAA ACTATGGTAT TAGAAAAAAC TCTCTCAGCT 
GTGTTTAAAG GTACTGGACC AGGAAAAAGT CAGTATCAAG TGGAGTTGAT AGATGGAAGC 
AACAACAGGA TAAACACCAT TTATCAATCT ACTGTCAAGG GTAAAACCTC ATTTTCATCT 
ATTCCTTCAA AAGTACCACC CAAACCTTCT GTAGGTTTTG AAGAAGATGC TTGTGAAGAA 
AATCCCTCTA ATGGACTTCA ACCAGTTGCC AACAGCTTCT CAAGACCTAC CAGAGATCTG 
AGTAGGTGGG ATAATAATCC ACAAAGAAAT ATGTATAATG AAGCATTTGT TAATAAGGGG 
AAACAATTTG GGAAAGCGAC TTCGCCTGGA AGTTCTTCAA ATGAAAATGA ATCACTATCC 
AGAAAAGACT TTGGTAGAAA GCAGTCCGGT ATTCAGCACT TGTCACATAC TCCTGACAAA 
AGTCAGTGGA GAGATCACCC CTCAAAATTT GGTAAAGCTG ATTGTAATCG TTCTCGAATA 
TCTAGTGGAG ATTCCAGATC TTCAGATGAG TCTAGTGGTA GCTTGAAGAG AAATATGAAA 
CATCCAAGAG GATCTTTCAC AGATGAAGCC CGATCTACAA AACGCTCTAT GTCAAACCAA 
ATTCAAGATT TAATTTCAAG GTCAAAAAAT CAAAACACTT CTTTACAAAG GGAAAATGTT 
GAAGTTAAAA TTTCCACCAA TTTTAAAGAA CAAGTAATAC CAGACAAAGC TTTTAGGGAT 
GTGACTGTTG TCTATGCAAT TAGTCCAAGT GAATTTTACT GTCAGTTGAC TGAACATGCT 
TCTGAACTCA TAGAATTAAT GAAGAAGTTG GAAGAAACGT ACAGTGAATT GAATGACAGC 
AGTTTGTCAT TGATTAATCC ACATGTTGGA AGTCCATGTG CAGCTTTTTA TTCAGCAGGT 
GGTGCTTGGT ATCGAGGGAA AGTTGTTGCC ATTCTAGACA CTTTTGTTGA GGTACAGTTT 
GTCGATTATG GAAATTCTGA GAAAGTTCCT ACCAATAAAG TTAAGAGTTT AAAATCTGAA 
TTTTTCTATT TGCCAATACA AGCAATTAAG TGCAGCTTGT ACAACACTCT TCCTCGGGAA 
TCATCATGGA CCATTGATGA TATCAATAAG TTTTCTGAAA TGACAAGTGA AAAATCTTTA 
GTGGGACAGT TTGTTCGCAA ACAGGTCAGT GGTAGTTTCT TAATTAATTT GATTGATATG 
GATAAATTAG AGAATGATAT TTTAAACAAG GATTTTGTTG AGTCTGGACA TGGAAAAACA 
CTTGGAGTGA AAACACCAAT TATAAGATCT CTGTCTTCTG CTATTGCAGA TGTGGAAATT 
GCAGATCCTT CAATAAGGTT AGGTGAAAAG GAACTTGTTC AGGTGACATG GGTTATGAAT 
CCTGGAGAGT TCTACTCCCA ACTTCAGTCA AGTAGTCAAC AATTTAACTA TTTAATGTGT 
GAAATTCAGG CTTTTTATGG GGCTTCAACT TCAGCGGCTA CATCTATTGA TCAGCCAAAG 
GTAAACCAAG TGTGTGCTGC AAGATTTACA GAAGATGATG CTTGGTATCG TGCAAGAATC 
CTTTATCTGA AAGATGGTAT GATTGGTGTT CTTTATGTTG ATTATGGAAA TGGCCAAGAA 
ATACCACGAG ATAGGATCAA GTACTTGATT CCAGATTTTG CAGCTTTACT TCCTTCCCAA 
GCTGTGAAGT GTTACTTAAA AGGTATCAAA CCACCTAATG ATAATTGGCC TTCTGTTGAT 
AAAAGCACGT ATGATAGTTT CTTTGAAGGA AAAACAGAAT GCACATTTAT TAAAAAGATA 
GATAGTGGAT TTTTAGTGGA CATGGAACAG AATGGAAAAA ACCTAGCTGA GGAGTTTATT 
TTAAAAAATT TAGCAGAAAG AGAAACTGCA TTAATTCCAG AACAGTCAGA ATTTAAAACA 
TGTGCCTATC TACAATCACC AAGGTTTGAA AACAAAACAA TTAAATTAAA TCAATCTGTA 
GAAGTTGTTG TATCTTTTGT GGAAAGTCCA CAGTCTTTTT GGTGTCAGTT AAAACAAGCC 
ATTCCTCAGC TTGATTCCTT GATGGAACAG ATAGATTCCT ACTACTCATC ACTCTCGGAT 
GATGAAGATG TTCTCTCTGC TTCAGAGAAG TTTTGTGTGG CAAAGTTTAG TGAAGATGAA 
AGTTGGTACA GAGCTTTTAT TCAGGAACAT GTATCACCAG AAACTGTAAG AGTCTACTAT 
CTTGATTATG GAAACTCAGA AGAGGTGGAA TTTTCTAAGT GTAAAAGCCT GCATGAAAAA 
TTCAAAGGTC TCTCAGCTCA AGCCATTAAG TGTCGACTCT ATGGCATGCC ATCTCAAAGT 
ACTGAACAAC AAAGCAAAGA TTTTGTGGAC ATAATTGATG AGAAAGAAGT GGTGATTAAA 
GTGCGTAAGA AGAAACCTAG CATACTGGCA GTGAATGTTT TGGATAATTC CTCAGGCACA 
GAAGTGGACA TTGGGGAGAC TGTTTATGGG ACCATGGAAG ATGAATGTCC TTTGAAAATC 
AATGGACAGC CAGATATTCC AGAAGGAGAA CAGGAAGGAT ATTTAATCAT AGATAGTTTA 
TATAATCTTG ATTTTGAATT CTTCCATGTG CAACTTAGTA GTGCAAAAAA CCAGATCAAC 
GATATGAGTT CTAGAATCAA AACAAGTGAA CTTTTATTGT CACACGAAGT AGAAATTGGT 
GAAAACTACT GTGTGCGAGA AAATGACATT TGGTATAGAG TCAAAGTGTA TCATAATAAT 
GGTACTGTTA TCTCAGCAAA AATGATAGAC TGTGGGAAAT ATTCATCCTT TGATGCTGAT 
ATATTTTATG AACTACCAGA AGATCTGGAA GCTTTTCCTC CTTTAGCCAT TCCCTGCAAA 
CTTTATGGGC TAGAGTGGAA GCGTTCAAAC AAAGAGTTAG TAGAAAAATG CTTGGCTGGC 
AGTGATTTGC TAAATGAGAA GAAATTCACC ATCAAATTCA TTAATAACTG GGAACAAGGA 
GCATTTGTTT CTATAATGGA TGGAGAAGTG GATATTAGTC AATCACTTGT CACTAAAGGT 
TTGGCAATGG CTATGTTTAA AGAAGATGAA GCTATGATTA CAGCTGGTTA TACTCTAACA 
ACCTTGAATA ATGACCCTTT GCTTGTCACT TTAAGTCATT TTGAGTCTAT AAACATGTTT 
TATGTGACAC TTGTAAAGGA GCTTAAAAAT CTTGATATCT TATCGGCAAA ACTTCAAGAT 
ACGTTTGAAG CTATGGACGA GGAAGAAGGT CAGGTGACAG AACCATGTTG GGGCAAAGCA 
TGTGTATGCA AGTTTAATGA AGATGGTGTG TGGTACAGAG GAGTAATTAC ATCAGTTGAT 
AAAGAAGTTG TCACTGTCTA TTTCGTAGAT TTTGGAAATG AGGAAGAAAC TACTATAGAC 
CAACTAAAGG AGTACCCAAA AGATATTTTG AATTCTAACA ACTTTGAGTC AGATTGCTTT 
AATTTTCCAG CCTTTGCTGT ATGTTGTTCC TTGTGTGACA CAACTTCAGT TGAGGGAAAA 
GAACAAAAAA TTGAGGAATT TTTAAAAGAA TTAGTTGGAA AGTATTTGAC TTTAGAGATT 
TGTGAAAGAG GTTCACCCTA CATTGTTTTG CTATCAATGA ATGGAATGGA AGTTAGTGAA 
ACTATGTGTA AGAAAGAACT TGTGAACTTA AACAAGCCAA CAGTTAACTA TACAGAAGGA 
AGTCGATCAC TGTCTGAGTA CAGATATTCT GTGGTCAGTC ATTTTGAGTC CTTGAGTTGC 
ATATTTCTTG TACCAAACTC ATATGAATAT GAAGCTTTCC AGAAAAAGTT ACAGACTGTA 
TACAACCAGA TTAAACTGGG AGAATTTGAA CTGGATAATG TGGCTAAAGG TGTACCATGT 
GTTGTAAAAT CAGCTATAGA TAACTGTTGG TACCGTGGCA TCATTACAGA TGTAAAGATG 
GAGACTGTAA CTAGTTCTAA AGAATTAAAA AAACTGAAGG TTTCTTTTTC CTGGCAGCTT 
CCTTTTTCTT TGAAATGTTC TTTGTCACAT GCTTTGGCTT GTGCTGAATT TAATCTAAAG 
CTGTTTAGTG AAAGTGATAA TGTAGAGGCA GTCAGAAAAG AAGTAATTGG AAAGATGGTA 
GAAGTTGAGT TTATGAGCAG TGAACCAATC CCAGCAGTGA AACTCCTTGT TGCAAGAGAT 
ATGTCTGAAA CAAATCAAGA TTATAATCAA ATAACTGACC TGATTGAAGA GGTATTAAAA 
TCAAAGTTTC TGCTCTCTTT GAAAGAGATG GAACAAGAAA AGGAACAGAG GTATTCACCA 
CTAACTACCC CACATCTACA GAAGTACCAA CCTTTAGATA TACCCTTGCA TACCAAGTTA 
GAAGTGAAGG TTAGTTATGT AAATACTCCT AGTGAGTTCT GGGTGCAGCT AGTTTACCAA 
CAAGAACAAC TTCACAACAT AATGGATGAA ATAGCAGACA TATATTCAAA TATCTTTGAA 
GATGAATTTG TTATTACAGA ACCTCAACCT GGGACTCCTT GTGTTTCTAT TTTCTCAGAA 
GATGGTGGAT GGTACCGAGG ATATGTCAAG GAAGTAATGG GTGGATCTGT AAAAATCCAT 
TTTGTGGATT ATGGAAATAG TGAAGAAGTT CCTCTTACTT TTGTGAGGGA GATCTCTTCA 
GATCTGTTGA AAACCCCCCA GCTAGCAGTC AAATGTTCTC TCCATGATGT TAAGCCTAAC 
AGTGACTCAG AATGGAATAT TGACCAGTGT GAAGTTTTCC AGGAAATAGT AAATGAAGAA 
AAGGTTTTTT TAGCAGAATT TTGCAGCAGT ATTGATAATA CATACCAAGT TAAACTGTTG 
GACATGGGAA TTGATGTAGG AAAGACCTTC TTGTCAAAAT GCATTGCTTC TAGCTCATCA 
GTACAAGAAG AAAAGTCGGA TATTGTTAAG CTGACACATA TTTTGCTTCA AGAACCTGTA 
CATGAAGCAG AATGTATAAG TCCTAATAAA GTTAAGTCAA ATGAAATCCT TAGTTTCAAT 
CAAATGGAGA ATGGAACCAC CAGTTTGTTT TCTAAAGAAA ATGATTCCTT CATTCTTAAA 
GAGATGGAAA ATGAATCATT CAGTGTTGTA AGTAACAGAA ATGTGTCATT CAGCATTACA 
GATAATGAAG AAGAATCATT TAGTGTTACA AATAATACTG ATTCATCAAA CTTTATGGGT 
ATTGGAAATG AGCAGTTCAG CATTATACAA AAAGAAGATT CATTTTATGA TGTAAACCAA 
GAATTTGAAT CATGTAATAT TATGGACAAG GACGAAGATT CATCCAGTGT TATAAATGAA 
GAAAAACCAA ACAGTGACAT GAACAAGCAA TATAGATCAG ACAGTGACAT GTATCAGTAT 
GATCCAACTA ATGTTATAAT GAAAAATGAA ATTTGTAACA TTGTTAAGGA AGATACTGGT 
GAGCCATCTG ATTTTGGTAA TGAATCAGAA AATTCTGTCA GTATCTGCAC ACCAGAGGAA 
GATATTTTAG AAAAAAATAT AAACCCAGAA GACTTTGCAT CATTGGGTGA ACCAAATCCT 
CTTTCCCAGC AAGGGGGTAA TCAACAGGAC CAGTTTGTCC CAGCAGTAAT CTCCATGAAG 
AAGTTAGATG AAGAAACTAC AGCAAGGACT GCTTATGAGA TTGAACCTAA TCTAGTTTCT 
GTTCAGTCTA AAGAAATTCT GGAAGATTGT ACATTGTTAG GAGAAAGTTT TAAAAATGAG 
TTTGTTCTTT CCAATAAAGA CATGACTGAG GTAAATCAAA GGTTTTTATC TAATGTGGAT 
AGAAACTTGA GTGATCATGA CAAGATAACA GAGTCATCAG ATGATTATTT GGAATACAAA 
AGTAATATAG AAGCAAAGAG TGGGGAGGAG TTAGAATATT GTACACATGA AGAGAAGTTA 
AGCCCATGGG AAGGGGAAAA TGTACCAGCA TCTACAAAAT TAGATGAAAC AATGCCACAT 
GAACAAGTAT CATTTTGTAA CAGAATTAGT AAAGAAGATA AAGATGTGGA AAATGAGTGT 
GAACAAGAAT TATCAACAAA TAGAGGACAA CAGTCAGAAC AGTTAATAAT AGCTTGTACT 
GAAAAAGCAT TAACTTCTAA CGACCAAGAA GAACAGCCCT TACTAAAAAA GGAATCAGCT 
AAAGAAATTG TTTGTAGTGA ACACACAACA CTAGATGGAG AGGAATCAGA TAAAGAAATT 
GTTTACAGTG AACACACAAC ACTAGAAGGA GAGGAATCAG ATAAAGAAAT TGTTTACAGT 
GAACACACAA CACTAGAAGG AGAGGAATCA GATAAAAAAA TTGTTTACAG TGAACACACA 
ACACTAGAAG GAAAGGAAAT AGATAAAGAA AATGTTTACA GTGAACATAC GACACTAGAA 
GGAAAGGAAT CAGATAAAGA AATTATTTGC AGTGAACATA CGACACTAGA AGGAAAGGAA 
TCAGATAAAG AAATTATTTG CAGTGAACAC ACAACACTAG AAGAAAAGGA ATTGGATAAA 
GAAATTGTTT GCAGTGAACA CATAACACTA GAAGAAAAGG AATTGGATAA AGAAATTGTT 
TGCAGTGAAC ACATAACACT AGAAGGAGAG GTATCAGATA AAGAAATTGT TTGCAGTGAA 
CACACAACAC TAGAAGAAGA GAAATCAGAT AAAGAAATTT ACAGTGAACA CACAACACTA 
GAAGGAGAGG AATCAGATAA AGAAATTGTT TACAATGAAC ACACAACACC AGAAGGAAAG 
GTATCAGATA AAGAAATTGT TTGCAGTGAA CACATAACAC TAGAAGGAAA AGACCTGGAT 
AACCTAAAAG ACATTGCTAG AGGTGATAAT CCAGAAGATG ATGATGATGA TTTAGGTACA 
ACCACTAATA AGCCTTCAGA AATCTCCACA CCCGATTCAC ATGGCAAAAC CTCATTTTCA 
ACAGGCGCAG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022243564.1
CDS	18..7415
Translation	MVTEVTNPNSFYCQLQNAVEQLETLTVDLEMYYGNLGPKDEVLTQPREDIPCVAQFSEDGAWYRALITNISTSGEYEVLFVDYGNSECCHQDVVKCIKPQFLNLPKQAVWCTLAGVPHLVWSTENREKFETMVLEKTLSAVFKGTGPGKSQYQVELIDGSNNRINTIYQSTVKGKTSFSSIPSKVPPKPSVGFEEDACEENPSNGLQPVANSFSRPTRDLSRWDNNPQRNMYNEAFVNKGKQFGKATSPGSSSNENESLSRKDFGRKQSGIQHLSHTPDKSQWRDHPSKFGKADCNRSRISSGDSRSSDESSGSLKRNMKHPRGSFTDEARSTKRSMSNQIQDLISRSKNQNTSLQRENVEVKISTNFKEQVIPDKAFRDVTVVYAISPSEFYCQLTEHASELIELMKKLEETYSELNDSSLSLINPHVGSPCAAFYSAGGAWYRGKVVAILDTFVEVQFVDYGNSEKVPTNKVKSLKSEFFYLPIQAIKCSLYNTLPRESSWTIDDINKFSEMTSEKSLVGQFVRKQVSGSFLINLIDMDKLENDILNKDFVESGHGKTLGVKTPIIRSLSSAIADVEIADPSIRLGEKELVQVTWVMNPGEFYSQLQSSSQQFNYLMCEIQAFYGASTSAATSIDQPKVNQVCAARFTEDDAWYRARILYLKDGMIGVLYVDYGNGQEIPRDRIKYLIPDFAALLPSQAVKCYLKGIKPPNDNWPSVDKSTYDSFFEGKTECTFIKKIDSGFLVDMEQNGKNLAEEFILKNLAERETALIPEQSEFKTCAYLQSPRFENKTIKLNQSVEVVVSFVESPQSFWCQLKQAIPQLDSLMEQIDSYYSSLSDDEDVLSASEKFCVAKFSEDESWYRAFIQEHVSPETVRVYYLDYGNSEEVEFSKCKSLHEKFKGLSAQAIKCRLYGMPSQSTEQQSKDFVDIIDEKEVVIKVRKKKPSILAVNVLDNSSGTEVDIGETVYGTMEDECPLKINGQPDIPEGEQEGYLIIDSLYNLDFEFFHVQLSSAKNQINDMSSRIKTSELLLSHEVEIGENYCVRENDIWYRVKVYHNNGTVISAKMIDCGKYSSFDADIFYELPEDLEAFPPLAIPCKLYGLEWKRSNKELVEKCLAGSDLLNEKKFTIKFINNWEQGAFVSIMDGEVDISQSLVTKGLAMAMFKEDEAMITAGYTLTTLNNDPLLVTLSHFESINMFYVTLVKELKNLDILSAKLQDTFEAMDEEEGQVTEPCWGKACVCKFNEDGVWYRGVITSVDKEVVTVYFVDFGNEEETTIDQLKEYPKDILNSNNFESDCFNFPAFAVCCSLCDTTSVEGKEQKIEEFLKELVGKYLTLEICERGSPYIVLLSMNGMEVSETMCKKELVNLNKPTVNYTEGSRSLSEYRYSVVSHFESLSCIFLVPNSYEYEAFQKKLQTVYNQIKLGEFELDNVAKGVPCVVKSAIDNCWYRGIITDVKMETVTSSKELKKLKVSFSWQLPFSLKCSLSHALACAEFNLKLFSESDNVEAVRKEVIGKMVEVEFMSSEPIPAVKLLVARDMSETNQDYNQITDLIEEVLKSKFLLSLKEMEQEKEQRYSPLTTPHLQKYQPLDIPLHTKLEVKVSYVNTPSEFWVQLVYQQEQLHNIMDEIADIYSNIFEDEFVITEPQPGTPCVSIFSEDGGWYRGYVKEVMGGSVKIHFVDYGNSEEVPLTFVREISSDLLKTPQLAVKCSLHDVKPNSDSEWNIDQCEVFQEIVNEEKVFLAEFCSSIDNTYQVKLLDMGIDVGKTFLSKCIASSSSVQEEKSDIVKLTHILLQEPVHEAECISPNKVKSNEILSFNQMENGTTSLFSKENDSFILKEMENESFSVVSNRNVSFSITDNEEESFSVTNNTDSSNFMGIGNEQFSIIQKEDSFYDVNQEFESCNIMDKDEDSSSVINEEKPNSDMNKQYRSDSDMYQYDPTNVIMKNEICNIVKEDTGEPSDFGNESENSVSICTPEEDILEKNINPEDFASLGEPNPLSQQGGNQQDQFVPAVISMKKLDEETTARTAYEIEPNLVSVQSKEILEDCTLLGESFKNEFVLSNKDMTEVNQRFLSNVDRNLSDHDKITESSDDYLEYKSNIEAKSGEELEYCTHEEKLSPWEGENVPASTKLDETMPHEQVSFCNRISKEDKDVENECEQELSTNRGQQSEQLIIACTEKALTSNDQEEQPLLKKESAKEIVCSEHTTLDGEESDKEIVYSEHTTLEGEESDKEIVYSEHTTLEGEESDKKIVYSEHTTLEGKEIDKENVYSEHTTLEGKESDKEIICSEHTTLEGKESDKEIICSEHTTLEEKELDKEIVCSEHITLEEKELDKEIVCSEHITLEGEVSDKEIVCSEHTTLEEEKSDKEIYSEHTTLEGEESDKEIVYNEHTTPEGKVSDKEIVCSEHITLEGKDLDNLKDIARGDNPEDDDDDLGTTTNKPSEISTPDSHGKTSFSTGAEE

Target ORF information:

RefSeq Version	XM_022387856.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus uncharacterized LOC106461035 (LOC106461035), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022387856.1

ORF Insert Sequence:

ATGGTGACAG AAGTTACAAA CCCAAATAGT TTTTATTGCC AGCTTCAGAA TGCAGTAGAA 
CAGTTGGAGA CATTGACTGT TGACCTTGAA ATGTATTATG GGAATCTTGG TCCAAAGGAT 
GAAGTTTTGA CACAACCTAG AGAGGATATT CCATGTGTAG CTCAGTTTAG TGAAGATGGT 
GCTTGGTATC GAGCTTTGAT CACTAACATT TCAACATCAG GGGAGTATGA GGTCTTATTT 
GTGGATTATG GCAATTCTGA ATGTTGTCAT CAAGATGTTG TAAAATGTAT TAAGCCTCAG 
TTTTTAAATC TTCCCAAACA AGCAGTTTGG TGTACACTTG CTGGTGTGCC TCATCTGGTA 
TGGTCAACTG AAAACAGAGA AAAATTTGAA ACTATGGTAT TAGAAAAAAC TCTCTCAGCT 
GTGTTTAAAG GTACTGGACC AGGAAAAAGT CAGTATCAAG TGGAGTTGAT AGATGGAAGC 
AACAACAGGA TAAACACCAT TTATCAATCT ACTGTCAAGG GTAAAACCTC ATTTTCATCT 
ATTCCTTCAA AAGTACCACC CAAACCTTCT GTAGGTTTTG AAGAAGATGC TTGTGAAGAA 
AATCCCTCTA ATGGACTTCA ACCAGTTGCC AACAGCTTCT CAAGACCTAC CAGAGATCTG 
AGTAGGTGGG ATAATAATCC ACAAAGAAAT ATGTATAATG AAGCATTTGT TAATAAGGGG 
AAACAATTTG GGAAAGCGAC TTCGCCTGGA AGTTCTTCAA ATGAAAATGA ATCACTATCC 
AGAAAAGACT TTGGTAGAAA GCAGTCCGGT ATTCAGCACT TGTCACATAC TCCTGACAAA 
AGTCAGTGGA GAGATCACCC CTCAAAATTT GGTAAAGCTG ATTGTAATCG TTCTCGAATA 
TCTAGTGGAG ATTCCAGATC TTCAGATGAG TCTAGTGGTA GCTTGAAGAG AAATATGAAA 
CATCCAAGAG GATCTTTCAC AGATGAAGCC CGATCTACAA AACGCTCTAT GTCAAACCAA 
ATTCAAGATT TAATTTCAAG GTCAAAAAAT CAAAACACTT CTTTACAAAG GGAAAATGTT 
GAAGTTAAAA TTTCCACCAA TTTTAAAGAA CAAGTAATAC CAGACAAAGC TTTTAGGGAT 
GTGACTGTTG TCTATGCAAT TAGTCCAAGT GAATTTTACT GTCAGTTGAC TGAACATGCT 
TCTGAACTCA TAGAATTAAT GAAGAAGTTG GAAGAAACGT ACAGTGAATT GAATGACAGC 
AGTTTGTCAT TGATTAATCC ACATGTTGGA AGTCCATGTG CAGCTTTTTA TTCAGCAGGT 
GGTGCTTGGT ATCGAGGGAA AGTTGTTGCC ATTCTAGACA CTTTTGTTGA GGTACAGTTT 
GTCGATTATG GAAATTCTGA GAAAGTTCCT ACCAATAAAG TTAAGAGTTT AAAATCTGAA 
TTTTTCTATT TGCCAATACA AGCAATTAAG TGCAGCTTGT ACAACACTCT TCCTCGGGAA 
TCATCATGGA CCATTGATGA TATCAATAAG TTTTCTGAAA TGACAAGTGA AAAATCTTTA 
GTGGGACAGT TTGTTCGCAA ACAGGTCAGT GGTAGTTTCT TAATTAATTT GATTGATATG 
GATAAATTAG AGAATGATAT TTTAAACAAG GATTTTGTTG AGTCTGGACA TGGAAAAACA 
CTTGGAGTGA AAACACCAAT TATAAGATCT CTGTCTTCTG CTATTGCAGA TGTGGAAATT 
GCAGATCCTT CAATAAGGTT AGGTGAAAAG GAACTTGTTC AGGTGACATG GGTTATGAAT 
CCTGGAGAGT TCTACTCCCA ACTTCAGTCA AGTAGTCAAC AATTTAACTA TTTAATGTGT 
GAAATTCAGG CTTTTTATGG GGCTTCAACT TCAGCGGCTA CATCTATTGA TCAGCCAAAG 
GTAAACCAAG TGTGTGCTGC AAGATTTACA GAAGATGATG CTTGGTATCG TGCAAGAATC 
CTTTATCTGA AAGATGGTAT GATTGGTGTT CTTTATGTTG ATTATGGAAA TGGCCAAGAA 
ATACCACGAG ATAGGATCAA GTACTTGATT CCAGATTTTG CAGCTTTACT TCCTTCCCAA 
GCTGTGAAGT GTTACTTAAA AGGTATCAAA CCACCTAATG ATAATTGGCC TTCTGTTGAT 
AAAAGCACGT ATGATAGTTT CTTTGAAGGA AAAACAGAAT GCACATTTAT TAAAAAGATA 
GATAGTGGAT TTTTAGTGGA CATGGAACAG AATGGAAAAA ACCTAGCTGA GGAGTTTATT 
TTAAAAAATT TAGCAGAAAG AGAAACTGCA TTAATTCCAG AACAGTCAGA ATTTAAAACA 
TGTGCCTATC TACAATCACC AAGGTTTGAA AACAAAACAA TTAAATTAAA TCAATCTGTA 
GAAGTTGTTG TATCTTTTGT GGAAAGTCCA CAGTCTTTTT GGTGTCAGTT AAAACAAGCC 
ATTCCTCAGC TTGATTCCTT GATGGAACAG ATAGATTCCT ACTACTCATC ACTCTCGGAT 
GATGAAGATG TTCTCTCTGC TTCAGAGAAG TTTTGTGTGG CAAAGTTTAG TGAAGATGAA 
AGTTGGTACA GAGCTTTTAT TCAGGAACAT GTATCACCAG AAACTGTAAG AGTCTACTAT 
CTTGATTATG GAAACTCAGA AGAGGTGGAA TTTTCTAAGT GTAAAAGCCT GCATGAAAAA 
TTCAAAGGTC TCTCAGCTCA AGCCATTAAG TGTCGACTCT ATGGCATGCC ATCTCAAAGT 
ACTGAACAAC AAAGCAAAGA TTTTGTGGAC ATAATTGATG AGAAAGAAGT GGTGATTAAA 
GTGCGTAAGA AGAAACCTAG CATACTGGCA GTGAATGTTT TGGATAATTC CTCAGGCACA 
GAAGTGGACA TTGGGGAGAC TGTTTATGGG ACCATGGAAG ATGAATGTCC TTTGAAAATC 
AATGGACAGC CAGATATTCC AGAAGGAGAA CAGGAAGGAT ATTTAATCAT AGATAGTTTA 
TATAATCTTG ATTTTGAATT CTTCCATGTG CAACTTAGTA GTGCAAAAAA CCAGATCAAC 
GATATGAGTT CTAGAATCAA AACAAGTGAA CTTTTATTGT CACACGAAGT AGAAATTGGT 
GAAAACTACT GTGTGCGAGA AAATGACATT TGGTATAGAG TCAAAGTGTA TCATAATAAT 
GGTACTGTTA TCTCAGCAAA AATGATAGAC TGTGGGAAAT ATTCATCCTT TGATGCTGAT 
ATATTTTATG AACTACCAGA AGATCTGGAA GCTTTTCCTC CTTTAGCCAT TCCCTGCAAA 
CTTTATGGGC TAGAGTGGAA GCGTTCAAAC AAAGAGTTAG TAGAAAAATG CTTGGCTGGC 
AGTGATTTGC TAAATGAGAA GAAATTCACC ATCAAATTCA TTAATAACTG GGAACAAGGA 
GCATTTGTTT CTATAATGGA TGGAGAAGTG GATATTAGTC AATCACTTGT CACTAAAGGT 
TTGGCAATGG CTATGTTTAA AGAAGATGAA GCTATGATTA CAGCTGGTTA TACTCTAACA 
ACCTTGAATA ATGACCCTTT GCTTGTCACT TTAAGTCATT TTGAGTCTAT AAACATGTTT 
TATGTGACAC TTGTAAAGGA GCTTAAAAAT CTTGATATCT TATCGGCAAA ACTTCAAGAT 
ACGTTTGAAG CTATGGACGA GGAAGAAGGT CAGGTGACAG AACCATGTTG GGGCAAAGCA 
TGTGTATGCA AGTTTAATGA AGATGGTGTG TGGTACAGAG GAGTAATTAC ATCAGTTGAT 
AAAGAAGTTG TCACTGTCTA TTTCGTAGAT TTTGGAAATG AGGAAGAAAC TACTATAGAC 
CAACTAAAGG AGTACCCAAA AGATATTTTG AATTCTAACA ACTTTGAGTC AGATTGCTTT 
AATTTTCCAG CCTTTGCTGT ATGTTGTTCC TTGTGTGACA CAACTTCAGT TGAGGGAAAA 
GAACAAAAAA TTGAGGAATT TTTAAAAGAA TTAGTTGGAA AGTATTTGAC TTTAGAGATT 
TGTGAAAGAG GTTCACCCTA CATTGTTTTG CTATCAATGA ATGGAATGGA AGTTAGTGAA 
ACTATGTGTA AGAAAGAACT TGTGAACTTA AACAAGCCAA CAGTTAACTA TACAGAAGGA 
AGTCGATCAC TGTCTGAGTA CAGATATTCT GTGGTCAGTC ATTTTGAGTC CTTGAGTTGC 
ATATTTCTTG TACCAAACTC ATATGAATAT GAAGCTTTCC AGAAAAAGTT ACAGACTGTA 
TACAACCAGA TTAAACTGGG AGAATTTGAA CTGGATAATG TGGCTAAAGG TGTACCATGT 
GTTGTAAAAT CAGCTATAGA TAACTGTTGG TACCGTGGCA TCATTACAGA TGTAAAGATG 
GAGACTGTAA CTAGTTCTAA AGAATTAAAA AAACTGAAGG TTTCTTTTTC CTGGCAGCTT 
CCTTTTTCTT TGAAATGTTC TTTGTCACAT GCTTTGGCTT GTGCTGAATT TAATCTAAAG 
CTGTTTAGTG AAAGTGATAA TGTAGAGGCA GTCAGAAAAG AAGTAATTGG AAAGATGGTA 
GAAGTTGAGT TTATGAGCAG TGAACCAATC CCAGCAGTGA AACTCCTTGT TGCAAGAGAT 
ATGTCTGAAA CAAATCAAGA TTATAATCAA ATAACTGACC TGATTGAAGA GGTATTAAAA 
TCAAAGTTTC TGCTCTCTTT GAAAGAGATG GAACAAGAAA AGGAACAGAG GTATTCACCA 
CTAACTACCC CACATCTACA GAAGTACCAA CCTTTAGATA TACCCTTGCA TACCAAGTTA 
GAAGTGAAGG TTAGTTATGT AAATACTCCT AGTGAGTTCT GGGTGCAGCT AGTTTACCAA 
CAAGAACAAC TTCACAACAT AATGGATGAA ATAGCAGACA TATATTCAAA TATCTTTGAA 
GATGAATTTG TTATTACAGA ACCTCAACCT GGGACTCCTT GTGTTTCTAT TTTCTCAGAA 
GATGGTGGAT GGTACCGAGG ATATGTCAAG GAAGTAATGG GTGGATCTGT AAAAATCCAT 
TTTGTGGATT ATGGAAATAG TGAAGAAGTT CCTCTTACTT TTGTGAGGGA GATCTCTTCA 
GATCTGTTGA AAACCCCCCA GCTAGCAGTC AAATGTTCTC TCCATGATGT TAAGCCTAAC 
AGTGACTCAG AATGGAATAT TGACCAGTGT GAAGTTTTCC AGGAAATAGT AAATGAAGAA 
AAGGTTTTTT TAGCAGAATT TTGCAGCAGT ATTGATAATA CATACCAAGT TAAACTGTTG 
GACATGGGAA TTGATGTAGG AAAGACCTTC TTGTCAAAAT GCATTGCTTC TAGCTCATCA 
GTACAAGAAG AAAAGTCGGA TATTGTTAAG CTGACACATA TTTTGCTTCA AGAACCTGTA 
CATGAAGCAG AATGTATAAG TCCTAATAAA GTTAAGTCAA ATGAAATCCT TAGTTTCAAT 
CAAATGGAGA ATGGAACCAC CAGTTTGTTT TCTAAAGAAA ATGATTCCTT CATTCTTAAA 
GAGATGGAAA ATGAATCATT CAGTGTTGTA AGTAACAGAA ATGTGTCATT CAGCATTACA 
GATAATGAAG AAGAATCATT TAGTGTTACA AATAATACTG ATTCATCAAA CTTTATGGGT 
ATTGGAAATG AGCAGTTCAG CATTATACAA AAAGAAGATT CATTTTATGA TGTAAACCAA 
GAATTTGAAT CATGTAATAT TATGGACAAG GACGAAGATT CATCCAGTGT TATAAATGAA 
GAAAAACCAA ACAGTGACAT GAACAAGCAA TATAGATCAG ACAGTGACAT GTATCAGTAT 
GATCCAACTA ATGTTATAAT GAAAAATGAA ATTTGTAACA TTGTTAAGGA AGATACTGGT 
GAGCCATCTG ATTTTGGTAA TGAATCAGAA AATTCTGTCA GTATCTGCAC ACCAGAGGAA 
GATATTTTAG AAAAAAATAT AAACCCAGAA GACTTTGCAT CATTGGGTGA ACCAAATCCT 
CTTTCCCAGC AAGGGGGTAA TCAACAGGAC CAGTTTGTCC CAGCAGTAAT CTCCATGAAG 
AAGTTAGATG AAGAAACTAC AGCAAGGACT GCTTATGAGA TTGAACCTAA TCTAGTTTCT 
GTTCAGTCTA AAGAAATTCT GGAAGATTGT ACATTGTTAG GAGAAAGTTT TAAAAATGAG 
TTTGTTCTTT CCAATAAAGA CATGACTGAG GTAAATCAAA GGTTTTTATC TAATGTGGAT 
AGAAACTTGA GTGATCATGA CAAGATAACA GAGTCATCAG ATGATTATTT GGAATACAAA 
AGTAATATAG AAGCAAAGAG TGGGGAGGAG TTAGAATATT GTACACATGA AGAGAAGTTA 
AGCCCATGGG AAGGGGAAAA TGTACCAGCA TCTACAAAAT TAGATGAAAC AATGCCACAT 
GAACAAGTAT CATTTTGTAA CAGAATTAGT AAAGAAGATA AAGATGTGGA AAATGAGTGT 
GAACAAGAAT TATCAACAAA TAGAGGACAA CAGTCAGAAC AGTTAATAAT AGCTTGTACT 
GAAAAAGCAT TAACTTCTAA CGACCAAGAA GAACAGCCCT TACTAAAAAA GGAATCAGCT 
AAAGAAATTG TTTGTAGTGA ACACACAACA CTAGATGGAG AGGAATCAGA TAAAGAAATT 
GTTTACAGTG AACACACAAC ACTAGAAGGA GAGGAATCAG ATAAAGAAAT TGTTTACAGT 
GAACACACAA CACTAGAAGG AGAGGAATCA GATAAAAAAA TTGTTTACAG TGAACACACA 
ACACTAGAAG GAAAGGAAAT AGATAAAGAA AATGTTTACA GTGAACATAC GACACTAGAA 
GGAAAGGAAT CAGATAAAGA AATTATTTGC AGTGAACATA CGACACTAGA AGGAAAGGAA 
TCAGATAAAG AAATTATTTG CAGTGAACAC ACAACACTAG AAGAAAAGGA ATTGGATAAA 
GAAATTGTTT GCAGTGAACA CATAACACTA GAAGAAAAGG AATTGGATAA AGAAATTGTT 
TGCAGTGAAC ACATAACACT AGAAGGAGAG GTATCAGATA AAGAAATTGT TTGCAGTGAA 
CACACAACAC TAGAAGAAGA GAAATCAGAT AAAGAAATTT ACAGTGAACA CACAACACTA 
GAAGGAGAGG AATCAGATAA AGAAATTGTT TACAATGAAC ACACAACACC AGAAGGAAAG 
GTATCAGATA AAGAAATTGT TTGCAGTGAA CACATAACAC TAGAAGGAAA AGACCTGGAT 
AACCTAAAAG ACATTGCTAG AGGTGATAAT CCAGAAGATG ATGATGATGA TTTAGGTACA 
ACCACTAATA AGCCTTCAGA AATCTCCACA CCCGATTCAC ATGGCAAAAC CTCATTTTCA 
ACAGGCGCAG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106461035 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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