LOC106460539 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106460539
Entrez Gene ID	106460539
Full Name	A disintegrin and metalloproteinase with thrombospondin motifs 20-like
Gene Type	protein-coding
Organism	Limulus polyphemus(Atlantic horseshoe crab)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013920256.1	XP_013775710.1	LOW QUALITY PROTEIN: A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like
XM_022387284.1	XP_022242992.1	A disintegrin and metalloproteinase with thrombospondin motifs 20-like isoform X2
XM_022387283.1	XP_022242991.1	A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC106460539 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106460539 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLi06055	XM_013920256.1 Latest version!	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like (LOC106460539), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLi110691	XM_022387283.1 Latest version!	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLi110692	XM_022387284.1 Latest version!	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106460539 ( XM_013920256.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_013775710.1 Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like (LOC106460539), mRNA.

Price & Availability↑

CloneID	OLi06055
Clone ID Related Accession (Same CDS sequence)	XM_013920256.1
Accession Version	XM_013920256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441641600000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	LOW QUALITY PROTEIN: A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666401.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT AACTGCCATT TCCTGTTAAA AGATAGAGTC 
ATAGACGGAA CCCCATGTGA TCAGGATACG TTCGACATTT GTGTGAATGG TGTATGTGAG 
AGTGCAGGGT GTGATCGCAT CCTAGGTTCC AACAAAACTC TAGACCGATG TGGAATTTGC 
GGAGGTGACA ATTCCACTTG CAAAACAATA CGTGGACACT TCAACGAGGT TAAGTATGGG 
TATCACTTGG TCGTCATGAT TCCAGCAGGA GCAAGTAACA TTGAAATTCT TCAATATGGT 
TACCAAAACT CTCCTCAAGA TGACAACTAT TTAGCTCTTG TGTCGAACAA TTATGAGTAC 
CTTCTTAATG GCAACTTCAC AGTGTCTCCA TTTAAGAAGA TTATTAAGTA TGCTGGCACT 
GCCTTGGAAT ACAGTGGGTC TGTAGCCATA ATAGAGCGTA TTAATACATC CAAACCACTC 
ATAAGAGAAT TGCATGTTCA GGTTCTTACT GTTGGTAATC TTCGTCCGCC CAACATTCAG 
TTTGAGTACA CTGTTTCTCT CCGAGATGAA AATAAATATC GTTGGGAGTT AGAGGAAAAG 
TGGTCAGAAT GTTCTCTCTT GTGTGAAGGT GAAACGTTCC AACAACCTGT CTGCAAAAGA 
ATATTTGACA GGCAAGTGGT CAACGAAAAT CATTGTGATG TTTCTAATAA GCCTTCTTAC 
GCCAAAATGT GCAATGAACA CTGTTCACTC AGATGGAACA TCTCTGTTAC ATCAGAATGT 
TCGTCGCAAT GTAGTCGTGG TACGAGACAA CGAGATGTTA AATGTTTGCA AGAATTTAGC 
AAACACAAAA CACTACAAGT TAATGATGAC TTCTGTGCAC ACCTTGGTGA TAAGCCAAGT 
CTTACAGAAT CCTGTGAAGG TAACTGCCCA GAGTCTCGTT GGGTGTTTGG AAAATGGTCT 
GCCTGTTCTA AATCCTGTGG AGGCGGCGAA CAAATTAGAA GCGCCACCTG TGTCGACTTG 
GACGGTAAAA AACTATCTGA ATTTCGCTGT GGCTCAGCGG AAAACCTCAA GAGGCAAAGT 
TGTAACACAG GGCCTTGTCC ACAGTGGATG ACTGGAGAGT GGACAGAATG TTCAATGTCA 
TGCGGTGCAG GCGAAAGACA CCGTCTTTAC TGGTGTGAAC ATGGCGGTAA AGCTATTAGT 
GGTATTTATT GCAATTCTAA GACGATCCCA AAACACAAAG ACATCTGTAA CCAACCAGCT 
TGTACTAAAT GGGATCATGG AAACTGGGGT CCGTGTTCTG TGACGTGTGG AGACGGCGTC 
AGCATGAGAG CTGTTCGTTG TCGTAAGTTG GACGGTGATA TTGTTCAAAA CTACCTCTGT 
GATACCAAGA CTCGGCCATT AAACTCAACA GCGTGCAATT TGCCACCTTG TATCCAAGCC 
ACTATCGCTT CAGCGACAAC AGAAACGTTA ACAAATCACC TCTTTAATTC CCGGCAGCAG 
AATAGAACAC CGTTTTTTAA ACAATCTTCC TTCACACCAT CATCCTCTAC TTCTATTCCA 
AGACGATCAG TGTGGAGATC GAGCAGTTGG TCTGAGTGTT CTGCGAGCTG CGGTGAGGGA 
ATGAGGATAC GTCACATCAC GTGCCATGAT GGCCATAGTA ACGTTTTACT TCCTGGGTCG 
TCTTGTGATG TGTCAAGTCG ACCAATCACT CTCGAAATCT GTAATGCTAA ACCTTGTGGA 
TCATGGAGAA CGTTAGAATG GAGGCAGTGT AGCGCAACTT GTGGACAAGG TGTGATGACA 
CGCAACGTTG GTTGCTTCTC CCTGGATGGA ACTCAACGTG AAGATCAAGA CTGTGACCAT 
GAAGCTCGCC CATGTTCACG AACATGTGGT AGTGGAACTC AACGTCGCCA GGTGGCATGT 
TATGATGAAT TGGGTAGAAT GAGCGATCAG TGTGAATTAG CGAGAAAGCC CATAGAAGTT 
TCACAATGCG GCGTGGACCA GTGCCCAGTT TGGTCAGCAG GGGAGTGGCA ACAGTGCAAC 
AAATCTTGTG GTGAAGGATT TCAAACCCGA GCTGTTACCT GTAGAGACAA AAGAGGACAA 
GTAATTCCGG AATACATGTG TGATGTTTAC GCCAGACCTT CAGAGTCCCG GAAGTGTAAC 
GCTAAACAAT GCCCGTTTTC TTACCCTGGT TATCGCTGGT ATCTCAGCTC TTGGAGTCCG 
TGTTCTGTGA CTTGTGGACA AGGTGTAGTA AGAAGAGAGA TAAGGTGCAT TGATGGATTA 
GAACGTTCAG TGTCACATGA GAATTGCAAC GGATCAATTC CAGAAAGTAT TAAACAATGC 
ATAAAACCAG TTTGTGCAGA GTGGAAGTGG TCGGAATGGA CAAAGTGCTC TACTAGGTGT 
GGAGACGGTG TAAGGATACG ACATTCAATA TGTAAAGAAG GCAATGTCAT AGTAGACAGC 
ACCAGATGTG AGGGTGAGAA ACCAAAAAAA CAATCTCAAA GTTGTTCTCA TCGACCGTGT 
AGCTACGACT GGTCGGTATC ACAGTGGTCC CAGTGTTCCG TGTCATGTGG TAAAGGTAGC 
CAACGTAGAG AGATCATGTG TCTTAATGAA TCCAAAGAGG TTGTTTCAAG AGATAATTGT 
GAAGCCCCAC CACCAGCTGA CGTCAGAGAA TGCAACCAGC CTGAATGTGC ACAGTGGTCT 
TGGACCGACT GGTCACAATG TTCTTCCACT TGTGGCCAAG GAAACCAGAT TCGCCATCAT 
CGATGTTTAC AGTCTAATAT TACAGTTAAT GACTCGGAAT GTGGAGAAAA TAGCTCCGAA 
CCGGAAACTC GTTCCTGCTC TAAAAAACCA TGCTTTTTCA AATGGGAGAA AGGAAATTGG 
AGCAAATGTA GTGTTACATG TGGAAAAGGA CAAAAACAGC GAGAAATCAG CTGCTTAGAT 
GGCAGTGGTG ACGTGGTGGC AGACAAGTTA TGTGAATCGA AACGAAAACC TAAAACCACA 
CGAGCATGTA GAAGGGGTCC AGCTTGCCCT TATTCATGGA TACCTGATGA ATGGACACAG 
TGTTCGAGCT CGTGCGGACC AGGCGTACAA ATGCGAAGAG CTCACTGCTT TCGAGTGAAT 
GCTTACGGTT GGATAGACCC TAACCCATTA CAAGAACCAC TTGACAACAA TGACACATGG 
TGTGACCGCA AGAAGCGCCC TCAAGGAGTT AGAATCTGCA ACTTAGGCGA CTGTGAAAAT 
GGAGCTGTGT GGAAACCCTG GCCATGGAAA TATTGTTCAA AGTCATGTGG TGTGGGAAAA 
CAAAGGCGTC GAGTTGCTTG TTATAACCTG GAAGGAAAAC GAATCGGAAG AAGAAACTGT 
GACCTCTCTT TGCGCCCAAA GACCAAACGC AAGTGTACTA TGAGACCATG TAAAGCAGTT 
AGCTGTAAAG ATGTCCAAGA ACGAACAGGG ATAAGAGAAG ATGGAGAGAA AGATTTGTAT 
GTACGTGGGA AGACACTCAA GATATATTGC GCTAACATGA ACACATCCGA CCCTCTGGAA 
TATGTGTCGC TACCTAGTGG AGATAGCAAC AATTATTCTG AAATGTATGA TAAAAGATTA 
ATAAGTCCCG GCACATGTCC CTACGACGGA ATTCGTCACA GTTCTTGCCC GTGTGTGACT 
GAGCGCAGGC TAGGATCAGG ACTGACAATG TTCAGCAAGT TGGCAATAAA CATCACAACT 
CTTCAGGTCC TCACTGATGA TTTCACCTTC AGTAAGACCT CACAAGGTCA TAGAGTAAGG 
TATGGAGAGG CTGGGGATTG TTACAGTCTC AACTCGTGTC CGCAAGGTCG TTTTGCTATC 
AATTTGGAAG GTACAGGTTT CATCGTGTCG GATGCTACTC AATGGAAAAA ATATGGTACC 
AGGCCTTCTG CCAGATTATA CATGTTACAG AGTAGAGAAG TAGTTCAAGG CAAATGTGGA 
GGTTATTGTG GAAAATGCAC ACCAGACGTC AGTAGTGGTC TTCTATTAGA TGTGGCACCT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013775710.1
CDS	749..6274
Translation	MFGLIHTSHGDYTIEPVPSRSESTNFDDTPQKAHHMIQLSLTHSSGKSRKGFMERCHLKDSARSKIKNKRETRNLNIGDEFHRNFNNSSKMRSKRSVSFERNVEVLVVVDRQMADQYREELRHYVLTLMSTVATIYKEPSLGNFINIAVVKLVTLTEEEDREIIHSSASKTLRSFCRWQRINNHDDDSHPHHHDTAVLITRKDLCRVSKACDTLGLANPGMVCSRYSSCAIVEDNGLSAAFTIAHELGHVLGIPHDDDFKCRRHKDQGQTLKVMARMLDEKSYPWDWSPCSRRYITNFLDSGNGECLIDKPTENLLIHNSSIAKTYPGQAYDMDYQCELVFGSDSKICPYMPVCGKLWCTLQGSNNYGCRTQHMPWADGTFCGLNKWCQRGECVKVDNRKQEPVDGQWGKWQRYGQCTRDCGGGIQKSVRECDNPRPVHGGKYCLGQRTRYLSCNTHSCPLGTPDPRAEECSTFNGKTMNFHGLPSSVNWLPFYSEDRSEACKLYCRVRGNCHFLLKDRVIDGTPCDQDTFDICVNGVCESAGCDRILGSNKTLDRCGICGGDNSTCKTIRGHFNEVKYGYHLVVMIPAGASNIEILQYGYQNSPQDDNYLALVSNNYEYLLNGNFTVSPFKKIIKYAGTALEYSGSVAIIERINTSKPLIRELHVQVLTVGNLRPPNIQFEYTVSLRDENKYRWELEEKWSECSLLCEGETFQQPVCKRIFDRQVVNENHCDVSNKPSYAKMCNEHCSLRWNISVTSECSSQCSRGTRQRDVKCLQEFSKHKTLQVNDDFCAHLGDKPSLTESCEGNCPESRWVFGKWSACSKSCGGGEQIRSATCVDLDGKKLSEFRCGSAENLKRQSCNTGPCPQWMTGEWTECSMSCGAGERHRLYWCEHGGKAISGIYCNSKTIPKHKDICNQPACTKWDHGNWGPCSVTCGDGVSMRAVRCRKLDGDIVQNYLCDTKTRPLNSTACNLPPCIQATIASATTETLTNHLFNSRQQNRTPFFKQSSFTPSSSTSIPRRSVWRSSSWSECSASCGEGMRIRHITCHDGHSNVLLPGSSCDVSSRPITLEICNAKPCGSWRTLEWRQCSATCGQGVMTRNVGCFSLDGTQREDQDCDHEARPCSRTCGSGTQRRQVACYDELGRMSDQCELARKPIEVSQCGVDQCPVWSAGEWQQCNKSCGEGFQTRAVTCRDKRGQVIPEYMCDVYARPSESRKCNAKQCPFSYPGYRWYLSSWSPCSVTCGQGVVRREIRCIDGLERSVSHENCNGSIPESIKQCIKPVCAEWKWSEWTKCSTRCGDGVRIRHSICKEGNVIVDSTRCEGEKPKKQSQSCSHRPCSYDWSVSQWSQCSVSCGKGSQRREIMCLNESKEVVSRDNCEAPPPADVRECNQPECAQWSWTDWSQCSSTCGQGNQIRHHRCLQSNITVNDSECGENSSEPETRSCSKKPCFFKWEKGNWSKCSVTCGKGQKQREISCLDGSGDVVADKLCESKRKPKTTRACRRGPACPYSWIPDEWTQCSSSCGPGVQMRRAHCFRVNAYGWIDPNPLQEPLDNNDTWCDRKKRPQGVRICNLGDCENGAVWKPWPWKYCSKSCGVGKQRRRVACYNLEGKRIGRRNCDLSLRPKTKRKCTMRPCKAVSCKDVQERTGIREDGEKDLYVRGKTLKIYCANMNTSDPLEYVSLPSGDSNNYSEMYDKRLISPGTCPYDGIRHSSCPCVTERRLGSGLTMFSKLAINITTLQVLTDDFTFSKTSQGHRVRYGEAGDCYSLNSCPQGRFAINLEGTGFIVSDATQWKKYGTRPSARLYMLQSREVVQGKCGGYCGKCTPDVSSGLLLDVAPP

Target ORF information:

RefSeq Version	XM_013920256.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like (LOC106460539), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013920256.1

ORF Insert Sequence:

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT AACTGCCATT TCCTGTTAAA AGATAGAGTC 
ATAGACGGAA CCCCATGTGA TCAGGATACG TTCGACATTT GTGTGAATGG TGTATGTGAG 
AGTGCAGGGT GTGATCGCAT CCTAGGTTCC AACAAAACTC TAGACCGATG TGGAATTTGC 
GGAGGTGACA ATTCCACTTG CAAAACAATA CGTGGACACT TCAACGAGGT TAAGTATGGG 
TATCACTTGG TCGTCATGAT TCCAGCAGGA GCAAGTAACA TTGAAATTCT TCAATATGGT 
TACCAAAACT CTCCTCAAGA TGACAACTAT TTAGCTCTTG TGTCGAACAA TTATGAGTAC 
CTTCTTAATG GCAACTTCAC AGTGTCTCCA TTTAAGAAGA TTATTAAGTA TGCTGGCACT 
GCCTTGGAAT ACAGTGGGTC TGTAGCCATA ATAGAGCGTA TTAATACATC CAAACCACTC 
ATAAGAGAAT TGCATGTTCA GGTTCTTACT GTTGGTAATC TTCGTCCGCC CAACATTCAG 
TTTGAGTACA CTGTTTCTCT CCGAGATGAA AATAAATATC GTTGGGAGTT AGAGGAAAAG 
TGGTCAGAAT GTTCTCTCTT GTGTGAAGGT GAAACGTTCC AACAACCTGT CTGCAAAAGA 
ATATTTGACA GGCAAGTGGT CAACGAAAAT CATTGTGATG TTTCTAATAA GCCTTCTTAC 
GCCAAAATGT GCAATGAACA CTGTTCACTC AGATGGAACA TCTCTGTTAC ATCAGAATGT 
TCGTCGCAAT GTAGTCGTGG TACGAGACAA CGAGATGTTA AATGTTTGCA AGAATTTAGC 
AAACACAAAA CACTACAAGT TAATGATGAC TTCTGTGCAC ACCTTGGTGA TAAGCCAAGT 
CTTACAGAAT CCTGTGAAGG TAACTGCCCA GAGTCTCGTT GGGTGTTTGG AAAATGGTCT 
GCCTGTTCTA AATCCTGTGG AGGCGGCGAA CAAATTAGAA GCGCCACCTG TGTCGACTTG 
GACGGTAAAA AACTATCTGA ATTTCGCTGT GGCTCAGCGG AAAACCTCAA GAGGCAAAGT 
TGTAACACAG GGCCTTGTCC ACAGTGGATG ACTGGAGAGT GGACAGAATG TTCAATGTCA 
TGCGGTGCAG GCGAAAGACA CCGTCTTTAC TGGTGTGAAC ATGGCGGTAA AGCTATTAGT 
GGTATTTATT GCAATTCTAA GACGATCCCA AAACACAAAG ACATCTGTAA CCAACCAGCT 
TGTACTAAAT GGGATCATGG AAACTGGGGT CCGTGTTCTG TGACGTGTGG AGACGGCGTC 
AGCATGAGAG CTGTTCGTTG TCGTAAGTTG GACGGTGATA TTGTTCAAAA CTACCTCTGT 
GATACCAAGA CTCGGCCATT AAACTCAACA GCGTGCAATT TGCCACCTTG TATCCAAGCC 
ACTATCGCTT CAGCGACAAC AGAAACGTTA ACAAATCACC TCTTTAATTC CCGGCAGCAG 
AATAGAACAC CGTTTTTTAA ACAATCTTCC TTCACACCAT CATCCTCTAC TTCTATTCCA 
AGACGATCAG TGTGGAGATC GAGCAGTTGG TCTGAGTGTT CTGCGAGCTG CGGTGAGGGA 
ATGAGGATAC GTCACATCAC GTGCCATGAT GGCCATAGTA ACGTTTTACT TCCTGGGTCG 
TCTTGTGATG TGTCAAGTCG ACCAATCACT CTCGAAATCT GTAATGCTAA ACCTTGTGGA 
TCATGGAGAA CGTTAGAATG GAGGCAGTGT AGCGCAACTT GTGGACAAGG TGTGATGACA 
CGCAACGTTG GTTGCTTCTC CCTGGATGGA ACTCAACGTG AAGATCAAGA CTGTGACCAT 
GAAGCTCGCC CATGTTCACG AACATGTGGT AGTGGAACTC AACGTCGCCA GGTGGCATGT 
TATGATGAAT TGGGTAGAAT GAGCGATCAG TGTGAATTAG CGAGAAAGCC CATAGAAGTT 
TCACAATGCG GCGTGGACCA GTGCCCAGTT TGGTCAGCAG GGGAGTGGCA ACAGTGCAAC 
AAATCTTGTG GTGAAGGATT TCAAACCCGA GCTGTTACCT GTAGAGACAA AAGAGGACAA 
GTAATTCCGG AATACATGTG TGATGTTTAC GCCAGACCTT CAGAGTCCCG GAAGTGTAAC 
GCTAAACAAT GCCCGTTTTC TTACCCTGGT TATCGCTGGT ATCTCAGCTC TTGGAGTCCG 
TGTTCTGTGA CTTGTGGACA AGGTGTAGTA AGAAGAGAGA TAAGGTGCAT TGATGGATTA 
GAACGTTCAG TGTCACATGA GAATTGCAAC GGATCAATTC CAGAAAGTAT TAAACAATGC 
ATAAAACCAG TTTGTGCAGA GTGGAAGTGG TCGGAATGGA CAAAGTGCTC TACTAGGTGT 
GGAGACGGTG TAAGGATACG ACATTCAATA TGTAAAGAAG GCAATGTCAT AGTAGACAGC 
ACCAGATGTG AGGGTGAGAA ACCAAAAAAA CAATCTCAAA GTTGTTCTCA TCGACCGTGT 
AGCTACGACT GGTCGGTATC ACAGTGGTCC CAGTGTTCCG TGTCATGTGG TAAAGGTAGC 
CAACGTAGAG AGATCATGTG TCTTAATGAA TCCAAAGAGG TTGTTTCAAG AGATAATTGT 
GAAGCCCCAC CACCAGCTGA CGTCAGAGAA TGCAACCAGC CTGAATGTGC ACAGTGGTCT 
TGGACCGACT GGTCACAATG TTCTTCCACT TGTGGCCAAG GAAACCAGAT TCGCCATCAT 
CGATGTTTAC AGTCTAATAT TACAGTTAAT GACTCGGAAT GTGGAGAAAA TAGCTCCGAA 
CCGGAAACTC GTTCCTGCTC TAAAAAACCA TGCTTTTTCA AATGGGAGAA AGGAAATTGG 
AGCAAATGTA GTGTTACATG TGGAAAAGGA CAAAAACAGC GAGAAATCAG CTGCTTAGAT 
GGCAGTGGTG ACGTGGTGGC AGACAAGTTA TGTGAATCGA AACGAAAACC TAAAACCACA 
CGAGCATGTA GAAGGGGTCC AGCTTGCCCT TATTCATGGA TACCTGATGA ATGGACACAG 
TGTTCGAGCT CGTGCGGACC AGGCGTACAA ATGCGAAGAG CTCACTGCTT TCGAGTGAAT 
GCTTACGGTT GGATAGACCC TAACCCATTA CAAGAACCAC TTGACAACAA TGACACATGG 
TGTGACCGCA AGAAGCGCCC TCAAGGAGTT AGAATCTGCA ACTTAGGCGA CTGTGAAAAT 
GGAGCTGTGT GGAAACCCTG GCCATGGAAA TATTGTTCAA AGTCATGTGG TGTGGGAAAA 
CAAAGGCGTC GAGTTGCTTG TTATAACCTG GAAGGAAAAC GAATCGGAAG AAGAAACTGT 
GACCTCTCTT TGCGCCCAAA GACCAAACGC AAGTGTACTA TGAGACCATG TAAAGCAGTT 
AGCTGTAAAG ATGTCCAAGA ACGAACAGGG ATAAGAGAAG ATGGAGAGAA AGATTTGTAT 
GTACGTGGGA AGACACTCAA GATATATTGC GCTAACATGA ACACATCCGA CCCTCTGGAA 
TATGTGTCGC TACCTAGTGG AGATAGCAAC AATTATTCTG AAATGTATGA TAAAAGATTA 
ATAAGTCCCG GCACATGTCC CTACGACGGA ATTCGTCACA GTTCTTGCCC GTGTGTGACT 
GAGCGCAGGC TAGGATCAGG ACTGACAATG TTCAGCAAGT TGGCAATAAA CATCACAACT 
CTTCAGGTCC TCACTGATGA TTTCACCTTC AGTAAGACCT CACAAGGTCA TAGAGTAAGG 
TATGGAGAGG CTGGGGATTG TTACAGTCTC AACTCGTGTC CGCAAGGTCG TTTTGCTATC 
AATTTGGAAG GTACAGGTTT CATCGTGTCG GATGCTACTC AATGGAAAAA ATATGGTACC 
AGGCCTTCTG CCAGATTATA CATGTTACAG AGTAGAGAAG TAGTTCAAGG CAAATGTGGA 
GGTTATTGTG GAAAATGCAC ACCAGACGTC AGTAGTGGTC TTCTATTAGA TGTGGCACCT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106460539 ( XM_022387283.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022242991.1 Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLi110691
Clone ID Related Accession (Same CDS sequence)	XM_022387283.1
Accession Version	XM_022387283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	A disintegrin and metalloproteinase with thrombospondin motifs gon-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666401.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT ACAACTGCCC ATTTCCTGTT AAAAGATAGA 
GTCATAGACG GAACCCCATG TGATCAGGAT ACGTTCGACA TTTGTGTGAA TGGTGTATGT 
GAGAGTGCAG GGTGTGATCG CATCCTAGGT TCCAACAAAA CTCTAGACCG ATGTGGAATT 
TGCGGAGGTG ACAATTCCAC TTGCAAAACA ATACGTGGAC ACTTCAACGA GGTTAAGTAT 
GGGTATCACT TGGTCGTCAT GATTCCAGCA GGAGCAAGTA ACATTGAAAT TCTTCAATAT 
GGTTACCAAA ACTCTCCTCA AGATGACAAC TATTTAGCTC TTGTGTCGAA CAATTATGAG 
TACCTTCTTA ATGGCAACTT CACAGTGTCT CCATTTAAGA AGATTATTAA GTATGCTGGC 
ACTGCCTTGG AATACAGTGG GTCTGTAGCC ATAATAGAGC GTATTAATAC ATCCAAACCA 
CTCATAAGAG AATTGCATGT TCAGGTTCTT ACTGTTGGTA ATCTTCGTCC GCCCAACATT 
CAGTTTGAGT ACACTGTTTC TCTCCGAGAT GAAAATAAAT ATCGTTGGGA GTTAGAGGAA 
AAGTGGTCAG AATGTTCTCT CTTGTGTGAA GGTGAAACGT TCCAACAACC TGTCTGCAAA 
AGAATATTTG ACAGGCAAGT GGTCAACGAA AATCATTGTG ATGTTTCTAA TAAGCCTTCT 
TACGCCAAAA TGTGCAATGA ACACTGTTCA CTCAGATGGA ACATCTCTGT TACATCAGAA 
TGTTCGTCGC AATGTAGTCG TGGTACGAGA CAACGAGATG TTAAATGTTT GCAAGAATTT 
AGCAAACACA AAACACTACA AGTTAATGAT GACTTCTGTG CACACCTTGG TGATAAGCCA 
AGTCTTACAG AATCCTGTGA AGGTAACTGC CCAGAGTCTC GTTGGGTGTT TGGAAAATGG 
TCTGCCTGTT CTAAATCCTG TGGAGGCGGC GAACAAATTA GAAGCGCCAC CTGTGTCGAC 
TTGGACGGTA AAAAACTATC TGAATTTCGC TGTGGCTCAG CGGAAAACCT CAAGAGGCAA 
AGTTGTAACA CAGGGCCTTG TCCACAGTGG ATGACTGGAG AGTGGACAGA ATGTTCAATG 
TCATGCGGTG CAGGCGAAAG ACACCGTCTT TACTGGTGTG AACATGGCGG TAAAGCTATT 
AGTGGTATTT ATTGCAATTC TAAGACGATC CCAAAACACA AAGACATCTG TAACCAACCA 
GCTTGTACTA AATGGGATCA TGGAAACTGG GGTCCGTGTT CTGTGACGTG TGGAGACGGC 
GTCAGCATGA GAGCTGTTCG TTGTCGTAAG TTGGACGGTG ATATTGTTCA AAACTACCTC 
TGTGATACCA AGACTCGGCC ATTAAACTCA ACAGCGTGCA ATTTGCCACC TTGTATCCAA 
GCCACTATCG CTTCAGCGAC AACAGAAACG TTAACAAATC ACCTCTTTAA TTCCCGGCAG 
CAGAATAGAA CACCGTTTTT TAAACAATCT TCCTTCACAC CATCATCCTC TACTTCTATT 
CCAAGACGAT CAGTGTGGAG ATCGAGCAGT TGGTCTGAGT GTTCTGCGAG CTGCGGTGAG 
GGAATGAGGA TACGTCACAT CACGTGCCAT GATGGCCATA GTAACGTTTT ACTTCCTGGG 
TCGTCTTGTG ATGTGTCAAG TCGACCAATC ACTCTCGAAA TCTGTAATGC TAAACCTTGT 
GGATCATGGA GAACGTTAGA ATGGAGGCAG TGTAGCGCAA CTTGTGGACA AGGTGTGATG 
ACACGCAACG TTGGTTGCTT CTCCCTGGAT GGAACTCAAC GTGAAGATCA AGACTGTGAC 
CATGAAGCTC GCCCAGTGAG TGAGAAGATA TGTAATCTTT CTGCATGCAA CAAAATTCTT 
GGAGTTTTAA GAGAAACTGG AGATATTACT CATAATAAAG TAGAAGGCTT TTTCTGGAGA 
ACAGGACCTT GGGGAGCATG TTCACGAACA TGTGGTAGTG GAACTCAACG TCGCCAGGTG 
GCATGTTATG ATGAATTGGG TAGAATGAGC GATCAGTGTG AATTAGCGAG AAAGCCCATA 
GAAGTTTCAC AATGCGGCGT GGACCAGTGC CCAGTTTGGT CAGCAGGGGA GTGGCAACAG 
TGCAACAAAT CTTGTGGTGA AGGATTTCAA ACCCGAGCTG TTACCTGTAG AGACAAAAGA 
GGACAAGTAA TTCCGGAATA CATGTGTGAT GTTTACGCCA GACCTTCAGA GTCCCGGAAG 
TGTAACGCTA AACAATGCCC GTTTTCTTAC CCTGGTTATC GCTGGTATCT CAGCTCTTGG 
AGTCCGTGTT CTGTGACTTG TGGACAAGGT GTAGTAAGAA GAGAGATAAG GTGCATTGAT 
GGATTAGAAC GTTCAGTGTC ACATGAGAAT TGCAACGGAT CAATTCCAGA AAGTATTAAA 
CAATGCATAA AACCAGTTTG TGCAGAGTGG AAGTGGTCGG AATGGACAAA GTGCTCTACT 
AGGTGTGGAG ACGGTGTAAG GATACGACAT TCAATATGTA AAGAAGGCAA TGTCATAGTA 
GACAGCACCA GATGTGAGGG TGAGAAACCA AAAAAACAAT CTCAAAGTTG TTCTCATCGA 
CCGTGTAGCT ACGACTGGTC GGTATCACAG TGGTCCCAGT GTTCCGTGTC ATGTGGTAAA 
GGTAGCCAAC GTAGAGAGAT CATGTGTCTT AATGAATCCA AAGAGGTTGT TTCAAGAGAT 
AATTGTGAAG CCCCACCACC AGCTGACGTC AGAGAATGCA ACCAGCCTGA ATGTGCACAG 
TGGTCTTGGA CCGACTGGTC ACAATGTTCT TCCACTTGTG GCCAAGGAAA CCAGATTCGC 
CATCATCGAT GTTTACAGTC TAATATTACA GTTAATGACT CGGAATGTGG AGAAAATAGC 
TCCGAACCGG AAACTCGTTC CTGCTCTAAA AAACCATGCT TTTTCAAATG GGAGAAAGGA 
AATTGGAGCA AATGTAGTGT TACATGTGGA AAAGGACAAA AACAGCGAGA AATCAGCTGC 
TTAGATGGCA GTGGTGACGT GGTGGCAGAC AAGTTATGTG AATCGAAACG AAAACCTAAA 
ACCACACGAG CATGTAGAAG GGGTCCAGCT TGCCCTTATT CATGGATACC TGATGAATGG 
ACACAGTGTT CGAGCTCGTG CGGACCAGGC GTACAAATGC GAAGAGCTCA CTGCTTTCGA 
GTGAATGCTT ACGGTTGGAT AGACCCTAAC CCATTACAAG AACCACTTGA CAACAATGAC 
ACATGGTGTG ACCGCAAGAA GCGCCCTCAA GGAGTTAGAA TCTGCAACTT AGGCGACTGT 
GAAAATGGAG CTGTGTGGAA ACCCTGGCCA TGGAAATATT GTTCAAAGTC ATGTGGTGTG 
GGAAAACAAA GGCGTCGAGT TGCTTGTTAT AACCTGGAAG GAAAACGAAT CGGAAGAAGA 
AACTGTGACC TCTCTTTGCG CCCAAAGACC AAACGCAAGT GTACTATGAG ACCATGTAAA 
GCAGTTAGCT GTAAAGATGT CCAAGAACGA ACAGGGATAA GAGAAGATGG AGAGAAAGAT 
TTGTATGTAC GTGGGAAGAC ACTCAAGATA TATTGCGCTA ACATGAACAC ATCCGACCCT 
CTGGAATATG TGTCGCTACC TAGTGGAGAT AGCAACAATT ATTCTGAAAT GTATGATAAA 
AGATTAATAA GTCCCGGCAC ATGTCCCTAC GACGGAATTC GTCACAGTTC TTGCCCGTGT 
GTGACTGAGC GCAGGCTAGG ATCAGGACTG ACAATGTTCA GCAAGTTGGC AATAAACATC 
ACAACTCTTC AGGTCCTCAC TGATGATTTC ACCTTCAGTA AGACCTCACA AGGTCATAGA 
GTAAGGTATG GAGAGGCTGG GGATTGTTAC AGTCTCAACT CGTGTCCGCA AGGTCGTTTT 
GCTATCAATT TGGAAGGTAC AGGTTTCATC GTGTCGGATG CTACTCAATG GAAAAAATAT 
GGTACCAGGC CTTCTGCCAG ATTATACATG TTACAGAGTA GAGAAGTAGT TCAAGGCAAA 
TGTGGAGGTT ATTGTGGAAA ATGCACACCA GACGTCAGTA GTGGTCTTCT ATTAGATGTG 
GCACCTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022242991.1
CDS	689..6340
Translation	MFGLIHTSHGDYTIEPVPSRSESTNFDDTPQKAHHMIQLSLTHSSGKSRKGFMERCHLKDSARSKIKNKRETRNLNIGDEFHRNFNNSSKMRSKRSVSFERNVEVLVVVDRQMADQYREELRHYVLTLMSTVATIYKEPSLGNFINIAVVKLVTLTEEEDREIIHSSASKTLRSFCRWQRINNHDDDSHPHHHDTAVLITRKDLCRVSKACDTLGLANPGMVCSRYSSCAIVEDNGLSAAFTIAHELGHVLGIPHDDDFKCRRHKDQGQTLKVMARMLDEKSYPWDWSPCSRRYITNFLDSGNGECLIDKPTENLLIHNSSIAKTYPGQAYDMDYQCELVFGSDSKICPYMPVCGKLWCTLQGSNNYGCRTQHMPWADGTFCGLNKWCQRGECVKVDNRKQEPVDGQWGKWQRYGQCTRDCGGGIQKSVRECDNPRPVHGGKYCLGQRTRYLSCNTHSCPLGTPDPRAEECSTFNGKTMNFHGLPSSVNWLPFYSEDRSEACKLYCRVRGTTAHFLLKDRVIDGTPCDQDTFDICVNGVCESAGCDRILGSNKTLDRCGICGGDNSTCKTIRGHFNEVKYGYHLVVMIPAGASNIEILQYGYQNSPQDDNYLALVSNNYEYLLNGNFTVSPFKKIIKYAGTALEYSGSVAIIERINTSKPLIRELHVQVLTVGNLRPPNIQFEYTVSLRDENKYRWELEEKWSECSLLCEGETFQQPVCKRIFDRQVVNENHCDVSNKPSYAKMCNEHCSLRWNISVTSECSSQCSRGTRQRDVKCLQEFSKHKTLQVNDDFCAHLGDKPSLTESCEGNCPESRWVFGKWSACSKSCGGGEQIRSATCVDLDGKKLSEFRCGSAENLKRQSCNTGPCPQWMTGEWTECSMSCGAGERHRLYWCEHGGKAISGIYCNSKTIPKHKDICNQPACTKWDHGNWGPCSVTCGDGVSMRAVRCRKLDGDIVQNYLCDTKTRPLNSTACNLPPCIQATIASATTETLTNHLFNSRQQNRTPFFKQSSFTPSSSTSIPRRSVWRSSSWSECSASCGEGMRIRHITCHDGHSNVLLPGSSCDVSSRPITLEICNAKPCGSWRTLEWRQCSATCGQGVMTRNVGCFSLDGTQREDQDCDHEARPVSEKICNLSACNKILGVLRETGDITHNKVEGFFWRTGPWGACSRTCGSGTQRRQVACYDELGRMSDQCELARKPIEVSQCGVDQCPVWSAGEWQQCNKSCGEGFQTRAVTCRDKRGQVIPEYMCDVYARPSESRKCNAKQCPFSYPGYRWYLSSWSPCSVTCGQGVVRREIRCIDGLERSVSHENCNGSIPESIKQCIKPVCAEWKWSEWTKCSTRCGDGVRIRHSICKEGNVIVDSTRCEGEKPKKQSQSCSHRPCSYDWSVSQWSQCSVSCGKGSQRREIMCLNESKEVVSRDNCEAPPPADVRECNQPECAQWSWTDWSQCSSTCGQGNQIRHHRCLQSNITVNDSECGENSSEPETRSCSKKPCFFKWEKGNWSKCSVTCGKGQKQREISCLDGSGDVVADKLCESKRKPKTTRACRRGPACPYSWIPDEWTQCSSSCGPGVQMRRAHCFRVNAYGWIDPNPLQEPLDNNDTWCDRKKRPQGVRICNLGDCENGAVWKPWPWKYCSKSCGVGKQRRRVACYNLEGKRIGRRNCDLSLRPKTKRKCTMRPCKAVSCKDVQERTGIREDGEKDLYVRGKTLKIYCANMNTSDPLEYVSLPSGDSNNYSEMYDKRLISPGTCPYDGIRHSSCPCVTERRLGSGLTMFSKLAINITTLQVLTDDFTFSKTSQGHRVRYGEAGDCYSLNSCPQGRFAINLEGTGFIVSDATQWKKYGTRPSARLYMLQSREVVQGKCGGYCGKCTPDVSSGLLLDVAPP

Target ORF information:

RefSeq Version	XM_022387283.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022387283.1

ORF Insert Sequence:

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT ACAACTGCCC ATTTCCTGTT AAAAGATAGA 
GTCATAGACG GAACCCCATG TGATCAGGAT ACGTTCGACA TTTGTGTGAA TGGTGTATGT 
GAGAGTGCAG GGTGTGATCG CATCCTAGGT TCCAACAAAA CTCTAGACCG ATGTGGAATT 
TGCGGAGGTG ACAATTCCAC TTGCAAAACA ATACGTGGAC ACTTCAACGA GGTTAAGTAT 
GGGTATCACT TGGTCGTCAT GATTCCAGCA GGAGCAAGTA ACATTGAAAT TCTTCAATAT 
GGTTACCAAA ACTCTCCTCA AGATGACAAC TATTTAGCTC TTGTGTCGAA CAATTATGAG 
TACCTTCTTA ATGGCAACTT CACAGTGTCT CCATTTAAGA AGATTATTAA GTATGCTGGC 
ACTGCCTTGG AATACAGTGG GTCTGTAGCC ATAATAGAGC GTATTAATAC ATCCAAACCA 
CTCATAAGAG AATTGCATGT TCAGGTTCTT ACTGTTGGTA ATCTTCGTCC GCCCAACATT 
CAGTTTGAGT ACACTGTTTC TCTCCGAGAT GAAAATAAAT ATCGTTGGGA GTTAGAGGAA 
AAGTGGTCAG AATGTTCTCT CTTGTGTGAA GGTGAAACGT TCCAACAACC TGTCTGCAAA 
AGAATATTTG ACAGGCAAGT GGTCAACGAA AATCATTGTG ATGTTTCTAA TAAGCCTTCT 
TACGCCAAAA TGTGCAATGA ACACTGTTCA CTCAGATGGA ACATCTCTGT TACATCAGAA 
TGTTCGTCGC AATGTAGTCG TGGTACGAGA CAACGAGATG TTAAATGTTT GCAAGAATTT 
AGCAAACACA AAACACTACA AGTTAATGAT GACTTCTGTG CACACCTTGG TGATAAGCCA 
AGTCTTACAG AATCCTGTGA AGGTAACTGC CCAGAGTCTC GTTGGGTGTT TGGAAAATGG 
TCTGCCTGTT CTAAATCCTG TGGAGGCGGC GAACAAATTA GAAGCGCCAC CTGTGTCGAC 
TTGGACGGTA AAAAACTATC TGAATTTCGC TGTGGCTCAG CGGAAAACCT CAAGAGGCAA 
AGTTGTAACA CAGGGCCTTG TCCACAGTGG ATGACTGGAG AGTGGACAGA ATGTTCAATG 
TCATGCGGTG CAGGCGAAAG ACACCGTCTT TACTGGTGTG AACATGGCGG TAAAGCTATT 
AGTGGTATTT ATTGCAATTC TAAGACGATC CCAAAACACA AAGACATCTG TAACCAACCA 
GCTTGTACTA AATGGGATCA TGGAAACTGG GGTCCGTGTT CTGTGACGTG TGGAGACGGC 
GTCAGCATGA GAGCTGTTCG TTGTCGTAAG TTGGACGGTG ATATTGTTCA AAACTACCTC 
TGTGATACCA AGACTCGGCC ATTAAACTCA ACAGCGTGCA ATTTGCCACC TTGTATCCAA 
GCCACTATCG CTTCAGCGAC AACAGAAACG TTAACAAATC ACCTCTTTAA TTCCCGGCAG 
CAGAATAGAA CACCGTTTTT TAAACAATCT TCCTTCACAC CATCATCCTC TACTTCTATT 
CCAAGACGAT CAGTGTGGAG ATCGAGCAGT TGGTCTGAGT GTTCTGCGAG CTGCGGTGAG 
GGAATGAGGA TACGTCACAT CACGTGCCAT GATGGCCATA GTAACGTTTT ACTTCCTGGG 
TCGTCTTGTG ATGTGTCAAG TCGACCAATC ACTCTCGAAA TCTGTAATGC TAAACCTTGT 
GGATCATGGA GAACGTTAGA ATGGAGGCAG TGTAGCGCAA CTTGTGGACA AGGTGTGATG 
ACACGCAACG TTGGTTGCTT CTCCCTGGAT GGAACTCAAC GTGAAGATCA AGACTGTGAC 
CATGAAGCTC GCCCAGTGAG TGAGAAGATA TGTAATCTTT CTGCATGCAA CAAAATTCTT 
GGAGTTTTAA GAGAAACTGG AGATATTACT CATAATAAAG TAGAAGGCTT TTTCTGGAGA 
ACAGGACCTT GGGGAGCATG TTCACGAACA TGTGGTAGTG GAACTCAACG TCGCCAGGTG 
GCATGTTATG ATGAATTGGG TAGAATGAGC GATCAGTGTG AATTAGCGAG AAAGCCCATA 
GAAGTTTCAC AATGCGGCGT GGACCAGTGC CCAGTTTGGT CAGCAGGGGA GTGGCAACAG 
TGCAACAAAT CTTGTGGTGA AGGATTTCAA ACCCGAGCTG TTACCTGTAG AGACAAAAGA 
GGACAAGTAA TTCCGGAATA CATGTGTGAT GTTTACGCCA GACCTTCAGA GTCCCGGAAG 
TGTAACGCTA AACAATGCCC GTTTTCTTAC CCTGGTTATC GCTGGTATCT CAGCTCTTGG 
AGTCCGTGTT CTGTGACTTG TGGACAAGGT GTAGTAAGAA GAGAGATAAG GTGCATTGAT 
GGATTAGAAC GTTCAGTGTC ACATGAGAAT TGCAACGGAT CAATTCCAGA AAGTATTAAA 
CAATGCATAA AACCAGTTTG TGCAGAGTGG AAGTGGTCGG AATGGACAAA GTGCTCTACT 
AGGTGTGGAG ACGGTGTAAG GATACGACAT TCAATATGTA AAGAAGGCAA TGTCATAGTA 
GACAGCACCA GATGTGAGGG TGAGAAACCA AAAAAACAAT CTCAAAGTTG TTCTCATCGA 
CCGTGTAGCT ACGACTGGTC GGTATCACAG TGGTCCCAGT GTTCCGTGTC ATGTGGTAAA 
GGTAGCCAAC GTAGAGAGAT CATGTGTCTT AATGAATCCA AAGAGGTTGT TTCAAGAGAT 
AATTGTGAAG CCCCACCACC AGCTGACGTC AGAGAATGCA ACCAGCCTGA ATGTGCACAG 
TGGTCTTGGA CCGACTGGTC ACAATGTTCT TCCACTTGTG GCCAAGGAAA CCAGATTCGC 
CATCATCGAT GTTTACAGTC TAATATTACA GTTAATGACT CGGAATGTGG AGAAAATAGC 
TCCGAACCGG AAACTCGTTC CTGCTCTAAA AAACCATGCT TTTTCAAATG GGAGAAAGGA 
AATTGGAGCA AATGTAGTGT TACATGTGGA AAAGGACAAA AACAGCGAGA AATCAGCTGC 
TTAGATGGCA GTGGTGACGT GGTGGCAGAC AAGTTATGTG AATCGAAACG AAAACCTAAA 
ACCACACGAG CATGTAGAAG GGGTCCAGCT TGCCCTTATT CATGGATACC TGATGAATGG 
ACACAGTGTT CGAGCTCGTG CGGACCAGGC GTACAAATGC GAAGAGCTCA CTGCTTTCGA 
GTGAATGCTT ACGGTTGGAT AGACCCTAAC CCATTACAAG AACCACTTGA CAACAATGAC 
ACATGGTGTG ACCGCAAGAA GCGCCCTCAA GGAGTTAGAA TCTGCAACTT AGGCGACTGT 
GAAAATGGAG CTGTGTGGAA ACCCTGGCCA TGGAAATATT GTTCAAAGTC ATGTGGTGTG 
GGAAAACAAA GGCGTCGAGT TGCTTGTTAT AACCTGGAAG GAAAACGAAT CGGAAGAAGA 
AACTGTGACC TCTCTTTGCG CCCAAAGACC AAACGCAAGT GTACTATGAG ACCATGTAAA 
GCAGTTAGCT GTAAAGATGT CCAAGAACGA ACAGGGATAA GAGAAGATGG AGAGAAAGAT 
TTGTATGTAC GTGGGAAGAC ACTCAAGATA TATTGCGCTA ACATGAACAC ATCCGACCCT 
CTGGAATATG TGTCGCTACC TAGTGGAGAT AGCAACAATT ATTCTGAAAT GTATGATAAA 
AGATTAATAA GTCCCGGCAC ATGTCCCTAC GACGGAATTC GTCACAGTTC TTGCCCGTGT 
GTGACTGAGC GCAGGCTAGG ATCAGGACTG ACAATGTTCA GCAAGTTGGC AATAAACATC 
ACAACTCTTC AGGTCCTCAC TGATGATTTC ACCTTCAGTA AGACCTCACA AGGTCATAGA 
GTAAGGTATG GAGAGGCTGG GGATTGTTAC AGTCTCAACT CGTGTCCGCA AGGTCGTTTT 
GCTATCAATT TGGAAGGTAC AGGTTTCATC GTGTCGGATG CTACTCAATG GAAAAAATAT 
GGTACCAGGC CTTCTGCCAG ATTATACATG TTACAGAGTA GAGAAGTAGT TCAAGGCAAA 
TGTGGAGGTT ATTGTGGAAA ATGCACACCA GACGTCAGTA GTGGTCTTCT ATTAGATGTG 
GCACCTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106460539 ( XM_022387284.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022242992.1 Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X2, mRNA.

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CloneID	OLi110692
Clone ID Related Accession (Same CDS sequence)	XM_022387284.1
Accession Version	XM_022387284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666401.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT ACAACTGCCC ATTTCCTGTT AAAAGATAGA 
GTCATAGACG GAACCCCATG TGATCAGGAT ACGTTCGACA TTTGTGTGAA TGGTGTATGT 
GAGAGTGCAG GGTGTGATCG CATCCTAGGT TCCAACAAAA CTCTAGACCG ATGTGGAATT 
TGCGGAGGTG ACAATTCCAC TTGCAAAACA ATACGTGGAC ACTTCAACGA GGTTAAGTAT 
GGGTATCACT TGGTCGTCAT GATTCCAGCA GGAGCAAGTA ACATTGAAAT TCTTCAATAT 
GGTTACCAAA ACTCTCCTCA AGATGACAAC TATTTAGCTC TTGTGTCGAA CAATTATGAG 
TACCTTCTTA ATGGCAACTT CACAGTGTCT CCATTTAAGA AGATTATTAA GTATGCTGGC 
ACTGCCTTGG AATACAGTGG GTCTGTAGCC ATAATAGAGC GTATTAATAC ATCCAAACCA 
CTCATAAGAG AATTGCATGT TCAGGTTCTT ACTGTTGGTA ATCTTCGTCC GCCCAACATT 
CAGTTTGAGT ACACTGTTTC TCTCCGAGAT GAAAATAAAT ATCGTTGGGA GTTAGAGGAA 
AAGTGGTCAG AATGTTCTCT CTTGTGTGAA GGTGAAACGT TCCAACAACC TGTCTGCAAA 
AGAATATTTG ACAGGCAAGT GGTCAACGAA AATCATTGTG ATGTTTCTAA TAAGCCTTCT 
TACGCCAAAA TGTGCAATGA ACACTGTTCA CTCAGATGGA ACATCTCTGT TACATCAGAA 
TGTTCGTCGC AATGTAGTCG TGGTACGAGA CAACGAGATG TTAAATGTTT GCAAGAATTT 
AGCAAACACA AAACACTACA AGTTAATGAT GACTTCTGTG CACACCTTGG TGATAAGCCA 
AGTCTTACAG AATCCTGTGA AGGTAACTGC CCAGAGTCTC GTTGGGTGTT TGGAAAATGG 
TCTGCCTGTT CTAAATCCTG TGGAGGCGGC GAACAAATTA GAAGCGCCAC CTGTGTCGAC 
TTGGACGGTA AAAAACTATC TGAATTTCGC TGTGGCTCAG CGGAAAACCT CAAGAGGCAA 
AGTTGTAACA CAGGGCCTTG TCCACAGTGG ATGACTGGAG AGTGGACAGA ATGTTCAATG 
TCATGCGGTG CAGGCGAAAG ACACCGTCTT TACTGGTGTG AACATGGCGG TAAAGCTATT 
AGTGGTATTT ATTGCAATTC TAAGACGATC CCAAAACACA AAGACATCTG TAACCAACCA 
GCTTGTACTA AATGGGATCA TGGAAACTGG GGTCCGTGTT CTGTGACGTG TGGAGACGGC 
GTCAGCATGA GAGCTGTTCG TTGTCGTAAG TTGGACGGTG ATATTGTTCA AAACTACCTC 
TGTGATACCA AGACTCGGCC ATTAAACTCA ACAGCGTGCA ATTTGCCACC TTGTATCCAA 
GCCACTATCG CTTCAGCGAC AACAGAAACG TTAACAAATC ACCTCTTTAA TTCCCGGCAG 
CAGAATAGAA CACCGTTTTT TAAACAATCT TCCTTCACAC CATCATCCTC TACTTCTATT 
CCAAGACGAT CAGTGTGGAG ATCGAGCAGT TGGTCTGAGT GTTCTGCGAG CTGCGGTGAG 
GGAATGAGGA TACGTCACAT CACGTGCCAT GATGGCCATA GTAACGTTTT ACTTCCTGGG 
TCGTCTTGTG ATGTGTCAAG TCGACCAATC ACTCTCGAAA TCTGTAATGC TAAACCTTGT 
GGATCATGGA GAACGTTAGA ATGGAGGCAG TGTAGCGCAA CTTGTGGACA AGGTGTGATG 
ACACGCAACG TTGGTTGCTT CTCCCTGGAT GGAACTCAAC GTGAAGATCA AGACTGTGAC 
CATGAAGCTC GCCCAGTGAG TGAGAAGATA TGTAATCTTT CTGCATGCAA CAAAATTCTT 
GGAGTTTTAA GAGAAACTGG AGATATTACT CATAATAAAG TAGAAGGCTT TTTCTGGAGA 
ACAGGACCTT GGGGAGCATG TTCACGAACA TGTGGTAGTG GAACTCAACG TCGCCAGGTG 
GCATGTTATG ATGAATTGGG TAGAATGAGC GATCAGTGTG AATTAGCGAG AAAGCCCATA 
GAAGTTTCAC AATGCGGCGT GGACCAGTGC CCAGTTTGGT CAGCAGGGGA GTGGCAACAG 
TGCAACAAAT CTTGTGGTGA AGGATTTCAA ACCCGAGCTG TTACCTGTAG AGACAAAAGA 
GGACAAGTAA TTCCGGAATA CATGTGTGAT GTTTACGCCA GACCTTCAGA GTCCCGGAAG 
TGTAACGCTA AACAATGCCC GTTTTCTTAC CCTGGTTATC GCTGGTATCT CAGCTCTTGG 
AGTCCGTGTT CTGTGACTTG TGGACAAGGT GTAGTAAGAA GAGAGATAAG GTGCATTGAT 
GGATTAGAAC GTTCAGTGTC ACATGAGAAT TGCAACGGAT CAATTCCAGA AAGTATTAAA 
CAATGCATAA AACCAGTTTG TGCAGAGTGG AAGTGGTCGG AATGGACAAA GTGCTCTACT 
AGGTGTGGAG ACGGTGTAAG GATACGACAT TCAATATGTA AAGAAGGCAA TGTCATAGTA 
GACAGCACCA GATGTGAGGG TGAGAAACCA AAAAAACAAT CTCAAAGTTG TTCTCATCGA 
CCGTGTAGCT ACGACTGGTC GGTATCACAG TGGTCCCAGT GTTCCGTGTC ATGTGGTAAA 
GGTAGCCAAC GTAGAGAGAT CATGTGTCTT AATGAATCCA AAGAGGTTGT TTCAAGAGAT 
AATTGTGAAG CCCCACCACC AGCTGACGTC AGAGAATGCA ACCAGCCTGA ATGTGCACAG 
TGGTCTTGGA CCGACTGGTC ACAATGTTCT TCCACTTGTG GCCAAGGAAA CCAGATTCGC 
CATCATCGAT GTTTACAGTC TAATATTACA GTTAATGACT CGGAATGTGG AGAAAATAGC 
TCCGAACCGG AAACTCGTTC CTGCTCTAAA AAACCATGCT TTTTCAAATG GGAGAAAGGA 
AATTGGAGCA AATGTTACAT GTGGAAAAGG ACAAAAACAG CGAGAAATCA GCTGCTTAGA 
TGGCAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022242992.1
CDS	692..5263
Translation	MFGLIHTSHGDYTIEPVPSRSESTNFDDTPQKAHHMIQLSLTHSSGKSRKGFMERCHLKDSARSKIKNKRETRNLNIGDEFHRNFNNSSKMRSKRSVSFERNVEVLVVVDRQMADQYREELRHYVLTLMSTVATIYKEPSLGNFINIAVVKLVTLTEEEDREIIHSSASKTLRSFCRWQRINNHDDDSHPHHHDTAVLITRKDLCRVSKACDTLGLANPGMVCSRYSSCAIVEDNGLSAAFTIAHELGHVLGIPHDDDFKCRRHKDQGQTLKVMARMLDEKSYPWDWSPCSRRYITNFLDSGNGECLIDKPTENLLIHNSSIAKTYPGQAYDMDYQCELVFGSDSKICPYMPVCGKLWCTLQGSNNYGCRTQHMPWADGTFCGLNKWCQRGECVKVDNRKQEPVDGQWGKWQRYGQCTRDCGGGIQKSVRECDNPRPVHGGKYCLGQRTRYLSCNTHSCPLGTPDPRAEECSTFNGKTMNFHGLPSSVNWLPFYSEDRSEACKLYCRVRGTTAHFLLKDRVIDGTPCDQDTFDICVNGVCESAGCDRILGSNKTLDRCGICGGDNSTCKTIRGHFNEVKYGYHLVVMIPAGASNIEILQYGYQNSPQDDNYLALVSNNYEYLLNGNFTVSPFKKIIKYAGTALEYSGSVAIIERINTSKPLIRELHVQVLTVGNLRPPNIQFEYTVSLRDENKYRWELEEKWSECSLLCEGETFQQPVCKRIFDRQVVNENHCDVSNKPSYAKMCNEHCSLRWNISVTSECSSQCSRGTRQRDVKCLQEFSKHKTLQVNDDFCAHLGDKPSLTESCEGNCPESRWVFGKWSACSKSCGGGEQIRSATCVDLDGKKLSEFRCGSAENLKRQSCNTGPCPQWMTGEWTECSMSCGAGERHRLYWCEHGGKAISGIYCNSKTIPKHKDICNQPACTKWDHGNWGPCSVTCGDGVSMRAVRCRKLDGDIVQNYLCDTKTRPLNSTACNLPPCIQATIASATTETLTNHLFNSRQQNRTPFFKQSSFTPSSSTSIPRRSVWRSSSWSECSASCGEGMRIRHITCHDGHSNVLLPGSSCDVSSRPITLEICNAKPCGSWRTLEWRQCSATCGQGVMTRNVGCFSLDGTQREDQDCDHEARPVSEKICNLSACNKILGVLRETGDITHNKVEGFFWRTGPWGACSRTCGSGTQRRQVACYDELGRMSDQCELARKPIEVSQCGVDQCPVWSAGEWQQCNKSCGEGFQTRAVTCRDKRGQVIPEYMCDVYARPSESRKCNAKQCPFSYPGYRWYLSSWSPCSVTCGQGVVRREIRCIDGLERSVSHENCNGSIPESIKQCIKPVCAEWKWSEWTKCSTRCGDGVRIRHSICKEGNVIVDSTRCEGEKPKKQSQSCSHRPCSYDWSVSQWSQCSVSCGKGSQRREIMCLNESKEVVSRDNCEAPPPADVRECNQPECAQWSWTDWSQCSSTCGQGNQIRHHRCLQSNITVNDSECGENSSEPETRSCSKKPCFFKWEKGNWSKCYMWKRTKTARNQLLRWQW

Target ORF information:

RefSeq Version	XM_022387284.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus A disintegrin and metalloproteinase with thrombospondin motifs 20-like (LOC106460539), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022387284.1

ORF Insert Sequence:

ATGTTTGGAT TAATTCATAC GTCTCATGGA GATTATACAA TTGAACCAGT GCCCTCTAGG 
AGTGAAAGTA CGAACTTTGA TGATACACCT CAGAAGGCAC ATCACATGAT TCAGTTAAGC 
CTGACTCATT CATCTGGAAA GAGCCGTAAA GGTTTTATGG AAAGGTGTCA TTTAAAAGAT 
TCTGCTAGGT CAAAAATAAA AAATAAACGG GAAACTAGGA ATTTGAATAT CGGAGATGAA 
TTTCATCGTA ATTTTAATAA TTCCTCCAAA ATGCGCTCCA AACGCTCAGT GTCTTTTGAG 
CGAAATGTGG AGGTGTTGGT TGTTGTTGAC AGGCAGATGG CTGATCAATA CCGTGAAGAG 
TTACGTCACT ATGTCCTGAC GTTAATGTCA ACGGTTGCAA CAATTTACAA GGAACCCAGT 
CTTGGAAATT TCATCAATAT TGCGGTGGTG AAACTGGTTA CACTAACTGA AGAAGAGGAT 
CGGGAAATCA TCCATTCAAG TGCTTCAAAG ACATTACGTA GTTTCTGCCG CTGGCAACGG 
ATAAACAATC ATGACGACGA CAGCCATCCC CATCATCATG ATACTGCTGT TTTGATCACA 
AGGAAAGATT TGTGTCGAGT CTCCAAAGCC TGTGACACTC TCGGTCTGGC CAACCCAGGA 
ATGGTATGTT CTCGCTACAG CAGTTGTGCA ATAGTTGAAG ACAATGGCCT TAGTGCCGCA 
TTTACAATAG CTCACGAGCT GGGACACGTA CTGGGAATTC CTCACGACGA TGATTTCAAG 
TGCAGGAGAC ACAAAGACCA GGGTCAAACT TTAAAAGTTA TGGCTAGGAT GTTAGACGAG 
AAAAGCTACC CATGGGACTG GTCTCCTTGT AGTAGGAGAT ACATCACCAA CTTTTTGGAC 
TCAGGTAATG GAGAATGTCT TATCGACAAG CCTACAGAGA ATCTACTAAT TCATAATTCT 
TCGATTGCGA AAACATACCC TGGGCAGGCA TATGACATGG ATTATCAATG CGAGTTGGTG 
TTCGGCAGCG ATTCGAAAAT ATGTCCCTAT ATGCCTGTGT GTGGAAAGTT ATGGTGCACT 
CTTCAGGGAA GTAATAACTA TGGTTGTCGG ACTCAACACA TGCCATGGGC CGACGGAACA 
TTTTGTGGTC TTAACAAGTG GTGCCAGCGT GGTGAATGTG TGAAAGTGGA CAATCGAAAA 
CAAGAACCTG TTGATGGTCA GTGGGGCAAG TGGCAAAGGT ACGGCCAATG TACTAGAGAC 
TGTGGAGGTG GTATTCAGAA ATCGGTGAGA GAATGTGACA ACCCTAGGCC TGTACACGGA 
GGAAAATATT GTTTAGGGCA AAGGACCCGA TATCTTTCTT GTAATACGCA TAGCTGCCCA 
CTTGGGACTC CAGATCCCAG GGCTGAAGAA TGCTCTACAT TTAACGGCAA GACCATGAAC 
TTTCATGGAC TGCCCTCTAG CGTCAACTGG CTCCCTTTTT ATTCTGAAGA TCGTTCAGAA 
GCCTGTAAAC TTTACTGTCG AGTGCGTGGT ACAACTGCCC ATTTCCTGTT AAAAGATAGA 
GTCATAGACG GAACCCCATG TGATCAGGAT ACGTTCGACA TTTGTGTGAA TGGTGTATGT 
GAGAGTGCAG GGTGTGATCG CATCCTAGGT TCCAACAAAA CTCTAGACCG ATGTGGAATT 
TGCGGAGGTG ACAATTCCAC TTGCAAAACA ATACGTGGAC ACTTCAACGA GGTTAAGTAT 
GGGTATCACT TGGTCGTCAT GATTCCAGCA GGAGCAAGTA ACATTGAAAT TCTTCAATAT 
GGTTACCAAA ACTCTCCTCA AGATGACAAC TATTTAGCTC TTGTGTCGAA CAATTATGAG 
TACCTTCTTA ATGGCAACTT CACAGTGTCT CCATTTAAGA AGATTATTAA GTATGCTGGC 
ACTGCCTTGG AATACAGTGG GTCTGTAGCC ATAATAGAGC GTATTAATAC ATCCAAACCA 
CTCATAAGAG AATTGCATGT TCAGGTTCTT ACTGTTGGTA ATCTTCGTCC GCCCAACATT 
CAGTTTGAGT ACACTGTTTC TCTCCGAGAT GAAAATAAAT ATCGTTGGGA GTTAGAGGAA 
AAGTGGTCAG AATGTTCTCT CTTGTGTGAA GGTGAAACGT TCCAACAACC TGTCTGCAAA 
AGAATATTTG ACAGGCAAGT GGTCAACGAA AATCATTGTG ATGTTTCTAA TAAGCCTTCT 
TACGCCAAAA TGTGCAATGA ACACTGTTCA CTCAGATGGA ACATCTCTGT TACATCAGAA 
TGTTCGTCGC AATGTAGTCG TGGTACGAGA CAACGAGATG TTAAATGTTT GCAAGAATTT 
AGCAAACACA AAACACTACA AGTTAATGAT GACTTCTGTG CACACCTTGG TGATAAGCCA 
AGTCTTACAG AATCCTGTGA AGGTAACTGC CCAGAGTCTC GTTGGGTGTT TGGAAAATGG 
TCTGCCTGTT CTAAATCCTG TGGAGGCGGC GAACAAATTA GAAGCGCCAC CTGTGTCGAC 
TTGGACGGTA AAAAACTATC TGAATTTCGC TGTGGCTCAG CGGAAAACCT CAAGAGGCAA 
AGTTGTAACA CAGGGCCTTG TCCACAGTGG ATGACTGGAG AGTGGACAGA ATGTTCAATG 
TCATGCGGTG CAGGCGAAAG ACACCGTCTT TACTGGTGTG AACATGGCGG TAAAGCTATT 
AGTGGTATTT ATTGCAATTC TAAGACGATC CCAAAACACA AAGACATCTG TAACCAACCA 
GCTTGTACTA AATGGGATCA TGGAAACTGG GGTCCGTGTT CTGTGACGTG TGGAGACGGC 
GTCAGCATGA GAGCTGTTCG TTGTCGTAAG TTGGACGGTG ATATTGTTCA AAACTACCTC 
TGTGATACCA AGACTCGGCC ATTAAACTCA ACAGCGTGCA ATTTGCCACC TTGTATCCAA 
GCCACTATCG CTTCAGCGAC AACAGAAACG TTAACAAATC ACCTCTTTAA TTCCCGGCAG 
CAGAATAGAA CACCGTTTTT TAAACAATCT TCCTTCACAC CATCATCCTC TACTTCTATT 
CCAAGACGAT CAGTGTGGAG ATCGAGCAGT TGGTCTGAGT GTTCTGCGAG CTGCGGTGAG 
GGAATGAGGA TACGTCACAT CACGTGCCAT GATGGCCATA GTAACGTTTT ACTTCCTGGG 
TCGTCTTGTG ATGTGTCAAG TCGACCAATC ACTCTCGAAA TCTGTAATGC TAAACCTTGT 
GGATCATGGA GAACGTTAGA ATGGAGGCAG TGTAGCGCAA CTTGTGGACA AGGTGTGATG 
ACACGCAACG TTGGTTGCTT CTCCCTGGAT GGAACTCAAC GTGAAGATCA AGACTGTGAC 
CATGAAGCTC GCCCAGTGAG TGAGAAGATA TGTAATCTTT CTGCATGCAA CAAAATTCTT 
GGAGTTTTAA GAGAAACTGG AGATATTACT CATAATAAAG TAGAAGGCTT TTTCTGGAGA 
ACAGGACCTT GGGGAGCATG TTCACGAACA TGTGGTAGTG GAACTCAACG TCGCCAGGTG 
GCATGTTATG ATGAATTGGG TAGAATGAGC GATCAGTGTG AATTAGCGAG AAAGCCCATA 
GAAGTTTCAC AATGCGGCGT GGACCAGTGC CCAGTTTGGT CAGCAGGGGA GTGGCAACAG 
TGCAACAAAT CTTGTGGTGA AGGATTTCAA ACCCGAGCTG TTACCTGTAG AGACAAAAGA 
GGACAAGTAA TTCCGGAATA CATGTGTGAT GTTTACGCCA GACCTTCAGA GTCCCGGAAG 
TGTAACGCTA AACAATGCCC GTTTTCTTAC CCTGGTTATC GCTGGTATCT CAGCTCTTGG 
AGTCCGTGTT CTGTGACTTG TGGACAAGGT GTAGTAAGAA GAGAGATAAG GTGCATTGAT 
GGATTAGAAC GTTCAGTGTC ACATGAGAAT TGCAACGGAT CAATTCCAGA AAGTATTAAA 
CAATGCATAA AACCAGTTTG TGCAGAGTGG AAGTGGTCGG AATGGACAAA GTGCTCTACT 
AGGTGTGGAG ACGGTGTAAG GATACGACAT TCAATATGTA AAGAAGGCAA TGTCATAGTA 
GACAGCACCA GATGTGAGGG TGAGAAACCA AAAAAACAAT CTCAAAGTTG TTCTCATCGA 
CCGTGTAGCT ACGACTGGTC GGTATCACAG TGGTCCCAGT GTTCCGTGTC ATGTGGTAAA 
GGTAGCCAAC GTAGAGAGAT CATGTGTCTT AATGAATCCA AAGAGGTTGT TTCAAGAGAT 
AATTGTGAAG CCCCACCACC AGCTGACGTC AGAGAATGCA ACCAGCCTGA ATGTGCACAG 
TGGTCTTGGA CCGACTGGTC ACAATGTTCT TCCACTTGTG GCCAAGGAAA CCAGATTCGC 
CATCATCGAT GTTTACAGTC TAATATTACA GTTAATGACT CGGAATGTGG AGAAAATAGC 
TCCGAACCGG AAACTCGTTC CTGCTCTAAA AAACCATGCT TTTTCAAATG GGAGAAAGGA 
AATTGGAGCA AATGTTACAT GTGGAAAAGG ACAAAAACAG CGAGAAATCA GCTGCTTAGA 
TGGCAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106460539 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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