LMJF_34_1130 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_34_1130
Entrez Gene ID	5654875
Full Name	conserved hypothetical protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001686154.2	XP_001686206.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_34_1130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_34_1130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb05263	XM_001686154.2 Latest version!	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_34_1130 ( XM_001686154.2 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686206.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLb05263
Clone ID Related Accession (Same CDS sequence)	XM_001686154.2
Accession Version	XM_001686154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007286). On Jun 1, 2012 this sequence version replaced XM_001686154.1. COMPLETENESS: incomplete on both ends.

ATGGGAGCGG GGCTGTCGCA GCAACCAACA CCCTCGTCGA AGCTCGCCTC GGCGCATCCG 
TCTTCTAGCG CTCCTGCGGA TGCGTGGAAG TCGAGCATGA GGGATGAGGT GCTGTGCGGG 
AAGCCACTGC GGCAGAGCTC GCCGGAAACC TCCGCAGCCG TGCCGCGGGA AAACCTGTAC 
AGCATCGTGG AGGAAGAAGA TATTGATATA GACTACGCAG ATGAAGAGAG TGTGCAGGCC 
TGTAAGGGCA GCGGTAGCGG CAGCGGCACA ACACACCCGC AGCACCGCTA TCCCGTCATG 
ATGCCGGGAG CCGGCTTAGC GAGGGTCGCT GTGCCGCCGA TACCCGTGAT GAGCGCCATC 
GCCGCACCAG CCAGCCCAGT GAGCGCCTGC AGCTCCACCG CCACCGATAC GCCACGGTCT 
ATCTTGAGAC AATCAGGCAC CGGAGGCGGC CGGACGCCGC GGTCGAACAG CCGCCGAATT 
AGCTTCGTGG GAGTCTCGTC TCCCCGCCCG GATGAGCACC GCTGCTGTCG CGATGGCGAT 
GAAGAAAGCG GAGACGACAG TGACGACTAC GAGTCAGCGG AGCCGTCGCT GCGCTACGAC 
ATCGTGACGC TGGCGATGTC TGCCACCTCA ACCCCGCCGC GGCACTCTCG GAAGGTACAC 
AGCCACGATC CTTCTCTGCA CAATGGTCAC TACCACAGCC ACGAACATGG CGAGGATGAC 
AGCAACGGGG CGTTGAGCCC GGTGCCGGCG TCACAGCACC AACAAAAGCG GACCTCCCCG 
TCACTTTCAC CGGAGCGTCC GCGCCTGTCC AGCCGCTCTC CAACCCACTC ACCGCCGCGC 
GACAGTGGAA GCGGATACAC AATTTCGGCC GGGGCGCCAT TATCGGCTGC CGTGACAGCC 
CCCTCCGCGC CTCCTCCGCC TGAGGAAGAG ACGGACGTGA TTACGCTTGA CGCGGCGCCG 
CAGCACCGCG CTCTTGACGA GCAGGATCTC TTGAAGAGCC CCAAACGAGG CGGCAATCAG 
CGTCGTGCGA AAAGCCCTTC GATGGGAACC AACGGACGCG ATGAGGACGT GTTGACCCAG 
CCGCACAATC GCAGCGAACC AAGCGGTAGC AGCCACGGCG CCGTTGTCGT GCGTCGCCTG 
CCTCCGCGGC AGCGCTGGCC ATCAGAGGCG TACCAAGATG AACCCCAAAC AGAGGTGAGC 
AAGACGCAGC GAGCGCGTCG ATCCCGGCGT GAGAGACTCG ACCCAGAGGC CGACATGGGC 
AGCAGCAGTA GCCGCTGCAG CAGCGACATG GGCTCGAGCT GCACTGGCAG CGACGACTCC 
TTGTCAGCAC GCTACGCCTC ACCCCTTAAG CTGCATCAAG AGGGGTATCG AGGGTCGAGC 
TACCAGTCGA CCTCATCATC GCCACCCGCG CTCGCCTGCG CAGCGGCAGT GGGAAGTGCA 
GGGGCACCGC CGATCGGTCA CCCCTCGCCG CATCGACGTA GTACAAGCGC GAGAAAGGAT 
CGTGATGGCA CTAGCAGCGA AAGAAGTCGA GAAAAGTCGT TGACGCCGCG GTCACCTCCG 
TCGCTGACAC AGCGAGACGA CACAGACGAG CCGATCCGCG GCAGCAGCGA CCACGATCAC 
CATCATAGAC ACTCTTCCAG AGGAGCGGAG ACAAGGTCTG GCCTGCCAGC AGAGGCTGGA 
GCAGCCCTAC CGACATCGCA CAACCGCGGT CGCGGCACGC GAGTGGGTGT GGAAACGCGA 
GGTCCGTACA CCCACGCAGT TGCCGCTGCC TTCCATCTGT GCAGAGAAGC GAGTTACTCT 
CCTTCGACCA TCACAGACCG CAGCTCCGGC CAGAGCGTGG CAGGCGACCT GTCTGGCTCC 
GTGTCCGAGG CGGCCGCGCA GGAGGCGAGA CGGGCACCGA GGAACTACCA GCAGCAGCAT 
GAGGTGGTAC CGTCAGCTCT ATCAGCTACG ACGGCGGCGG CGCCACTGAG GCTATCCAAG 
GATAGGAAGG TCAAAAAAGC AGTGGTTGCC GAAGAGCCGC AGGTGTACGT GGATCCGCTC 
TTGCTCGAGC GTCGACATCG CCACGAGCTC ACGCGGCAAG AGTCGCCGTC GCATGTAGAG 
CGGCACTCGG GGCTCCACGC GCGCCGCAGG CGCCCGGGGT CTAGAACTGC GGCGCAGCAG 
CAGCCAACCA CCTTCCAGTC AGTGAGCTTG CGGGACATAA TGATGCGCCA CGACTCGCCG 
GAGACCAAAT CACGACGCGG CACGCCATCC AGTCAGTCCA CTGCTCCGGC GATATTACGG 
CTTCCTTCTG ACGTGCGGCA GCTTCAGCTC CAACACAACG GGGATGGCGA CGATGATGAT 
GCAGCTCACA CCCTTACACC CGGCGCGGTA GCCTTGGAAG CATCTGTTGC GTCCTCTTCG 
CTCCTCTCTA GTGGTGGCGT GCCGGCCGAT GAGCTTTTCT GGGACGAGCC GACGCCAACT 
TCGCGCGCCA GCAGCCGTTC ACCGCCCCCT GTTTCCCCAA GATCCTCCTC AACGCTGGCA 
CCCAACACTC CCGACAGACA CAAAGTGCTT CGCAAGAAGC TTGTTGTCGT CGTGCGCCGA 
AAGAAGAATG GCTCAGCACA GTCAGAGAAC GATCCTGTGG TACAGATGTC GCTGCTGCCC 
TTCAAGGAGG AAACTGCAGC TCTACTGAAA GCGAACGCCT CGCTGCAAGG GTCACCGCAG 
CAGCGACGCG CGCACAGCTC CGGCACCTCT GCCGCGGCAA CCGTTCCGAC TCTGGAGAAG 
CTGATCAACA AAGTGGAGCG AGGCGAGAGT GCCTCCGTGT TCTCCCGCTG CCTCAGCCGC 
GCATCTGAAC ATCAAGGCCA GAGGCCGCGT GAACTTTCGG AGAGGCGCGA CGGAGGCGAC 
GACCACGCCG GTGAAAACGA GATCAGCGAG GAAAACCGTA GGCGGTTCCG GCATGCGCTG 
TCAGTGTTGT CATCTCGAAG TGTGAGCCCG CGCGCGTCTG TCTCACCATC GACTACGTCG 
AAGGCGGGGC GGTCTCAACG AAGTCGCACA CGCTCACCAG ACTCACAAAT CAATGCCTCA 
TACGCTTGCG CGCGACCACT TCGCTGCAGC GGCAGCGCTA GCGAGGAGTC CGTATCGCAC 
ACTGGCGATG CCGCGCTGAG AAGGAGCAGT GGCCGCAGGC ATATATCTCA CGTGGCGGCG 
GTGAGCGAGA CGGCAGCGGC GAGCATAGGT GCGACATCGC CGCCCCAGCG TCGATCTGCC 
CGTGGTGGCT CACGTCACTC CGACGAGAAG TCGGCGCGTA GGTCCGAGGG TGGGCATCGC 
CACAAGTCGC GGCGGAGACA TAGTCACGAG AGCATAGCAC ACAGCACCGC TGGGCACCAG 
CGGCGTTCTC TTTCAGGCGC ATCCATGCCT GGCGCACCGT ATTGTGCGGC GCAGAACGAC 
GAGTTCTACG GCGCCGCGGA CATGGCAAAG GATGTGGCGA CGCTCTTTCC ATCACCTTTT 
CAGGAGCGCA TCGGCATCAC ACCGATGTCA ATGCCGCTTC TACCGCGGCT GGTGCTGCGA 
GCGGAGCTTG GTGCGCTGCC GCAAGGGCAA TATGCCCATC ACCCGCACAG TAACATCGAC 
GCAGCTCAGC GCACCAACGG TTCGTGGTCC GCCTCCGATG ACGAGGATGA GCATGCCATG 
GGCCGAATCA CCGATAGCGA AAGCGGTGGG CGTCGTTCAC GTCCCCTGAT GCTGCAGTTC 
AGCAGTGGCA CGCAAACGGA TCGGGCGACA GGAGTCAACC ATGCTTTTGG TAGCGCTCGC 
AGTGGGATGC CTCTCTTAGC GGCGACGCCA GTGCAGAGGG CTGCCGCCAT TGGGCCGATC 
GGAGGCACGC TTTCCTCAGC TCACCCCGGC AAACCCGTTG GCACGTCAGT GCCAACAATC 
GGCGCTGGTC TGGCGGCATC CTTACTCGCA CCAACGCACA ATGCAGCTGG CGATGCCGTG 
TTCAACGAAG AGATGGCGTT AGCTGTCGAA AAGCGTGCAG CGAACTCGCC GCTCGTCGCG 
GAGGCTTCCG CTACAGAGTC GCCAGCATCG TTTGTAAAGC GGGCGGCGTC ACCGGCAGCG 
GCAGCGAAGC CCTTCACCGA CTCCAACTCG CGTATTCTCA GCGGCAGTGG CAGCGGCGCT 
GCTAAAGGAA GAGGTGAGGC CGCGGTCTTT GGCGGCAATG TCGGGGCATC GAGGGCGGCG 
TACGCCCCTC TATTTTCGGC GACCTCAGCG CACGTGGCCG CGTCCAGCTC TAGGCCGGCC 
GCCCAGGACC TTAGCACCGC AGCACGTGCA ATGATGGATG CCACCACCAG TGTGCCGCTC 
TTTTTTCCGC CGCTGACTGT CGACAACGTG TCACAGTTAT CTCACGACTG GCAGTCCACG 
CTGCGTGAGC GCTCGAGCAA GGCGAAGCGG AGTGTGATGG AGAGCTTTCT GATTGCGTCG 
GCCGAGGCTG GTGCGGCGAC AACGGCAGCT GCTGTCGCTG CTGCTGCACC GTCACCACCG 
CTGTACCTCG CGGACCCCGC CGCCACTGAC GTAAACCCTT CGGACATGTG CGCTGGTGGT 
TGCGGAGGCC TTACCGTCCC TGCTGTTGGT GCTGAGGCTC CCTCTGAGTG GCAGCAACGA 
GCCAGACCAG CACAGGCACA CCAGGGTCTT GCGGTAGAGG AATTTTATGG GCGTTCTGTC 
ACCTCAGCTG CCATTGCCAA TGCGCCGCGC TCCGCGCATT CCCTCCCGCC ACGATCTGGA 
CCCCAGTCTT TCTCGGAAAT CAAGTGCGAC GTGTGGCAAC GCAGTGGCAG CCCACAATTG 
GCGGCGCAGC GGGAGCCTGA GCAGCAGCAG GCAACGAGGT CTCCACCGTT TTCAGCACCC 
TCGGAAAACA AGTCCGTGCA GGGCAAATCT GCAATGCCGG TAGGCAGTGT TTCGAAAAGC 
TGTGCAGCCT CCAAGAGCAG AGACGGCGTA GAGGAACGTT CTACGTCAGC ACGCAAGCAC 
AGTCGCCGTC GACGTCGCCG CGCTTCGCCG GAACGTCGCG CCAACAGCAA TGGCGACCAG 
CTCACAGACG CCCTTAATGC GGAGACCCTG TCGACAGTGG CAGCCGACTC GCCATGCAAG 
AGCAGCAGCA GCGCTGAAAA GATCAACATG GTTGGCGACG TCACTGCGCT GGCGGGGGTT 
TCTCCACAAC CTCTGTCGAG GGAGTCTCTC CCCGTGCCTG CCACACTTGG CGAAGCGAAG 
CAGAAGGCCC AGCAGGGTGA CCAGACGAGC GACAGCAGCA GCCGACCGTG TTCTGCAGTA 
GGAAACGGAA CGGCGGCCAA CGCAGCTGTC GCCACCGCTA CCGACGGTGT GGGTGCCCGC 
TCTGCGCCTC CGCCGCCGCC GCCTTTCTTC TCCATCGTTG AGGCTGCCGC GGAGTCACGC 
GCGTCGCGCT CGGTGGAGAA GGCAGCGGAA AAGGGCCGCG GTAATCATCA ACGAGACGAG 
CGTCGCGAAC ACCTCAGCCG TCACTCTCCC TCGCCCTTCT CCTCGGAGGC ATCGCAGTCG 
CCGAGCAGCA AGCGACGGAA AACGTCGAGT AAGCACAAGA CAAAGCACGA CGGCGACGAC 
AAGCATAAGA AGCGGAAGCA TGGCCATGGG CGTCATGGCC GTTACAGTCG CAGTGGCACG 
CGCTCGCTAT CGGGTCAGAC CTGTGACACC GCCGCCGCTG AAATTGACAG TGACAGCGAC 
GTGGACAGCA ATCTCACGAC ATCGATAGAG TTCCAGCTGT CTCGGCTGCT ACTGCTGCGC 
GAGGCGGCAG TGTTCACGAA GCCGCCTCTC TCTGCGGCCG TTGACGCGTC AAGGCGAAAC 
ACGCGCAAGA AAGACAAGGC AGATGGAAAG GCAGCAAGAA AGGCTGCTGC AGCGAAGGAC 
GAGCACCACC GCCATCGCAA CCGGAGTTCA AAGGAGAAGA GAAAGAGGTC ATCGAAGAGG 
GCCGCTAAGC AGCGCAGCAA GTCGCCTGCG GCCACCACCG AGTCGCGGCT CAGCGACGGC 
GCGGACAGGA AGAAAAAAAA GAGGGAAAAA GCATACCACT GCTCAACCTC TCCGTCAAAG 
CCGGGCCCTC TGCCGCCACC GCCCCCGCAC CAGCGTTCGC GCGCCTTGTA CGGTGAGGAG 
GCCGCGGCAA GCGCTGCAGC GTCGCTTTCG GACGTGGCCT CCCGCTACCG CCTCGGCGCC 
CCTGGCTGCA GTCCCCGCGA CGACGGCGCC GCTTTCGCAT CGACGCGCCC CTACTTCAGG 
GTCGGCAGGA TCTACCGCGG AGGTCAGGGA GAGGACGCGG AGGCGCCTTA TGCACATCAT 
CGCGCCGCAG CCCACACTGG AGCAGACTCG GAAACCCCGT ATGAACGCTA TCGTCGGCCC 
TCTCACACCT CGCCACTGCG CGGCCAACGC TCCTACGTGG CACCGACAGC ATCGGGATGG 
GCAGACGGTT TTCGCCCAGC CTGCAGACCC GCGCCACGCC CTGCCGCCAC CGCCGAGGAT 
CCCTCCTTGT GGCATCGCAG CTACAGCAGC GCAAGCCGTC CCTCGACCCG CTGGAATGAC 
TACGTTCGAG ATGCAGAGCG CGGCGCCGCT GGCAGGTCGT CGTCCTCTCC GTACATGCGC 
GCTCATCACT CCCGGTCCCT TGGTCAGCGA CGACGCGGTA GTGACGCTGG CATCCCTACG 
GCAGGGGAGT GTGGTCGCGG GAGTAGTGCC GAGGGAGGCT CGCCCAGCTC AGAACAACCG 
GGCTCCGGCA CCTACTCTGC CACCTACGCA AGCCGTCGTC GGCAGACGGA TGAGTTTGAC 
GATATCAACG TCGCGCCGAT ACGACAGTAC TTCAACGACC ATGGCCAAGT CGGCGCCGAC 
GAGCGCGGCG GCGGTGTGCA GTACGGCACC ACCTCAGCCT TTGAGCGGCA TCCCAGTCGG 
TCAGCCGCCT CCAACCGCGC ACCGACGGCC CGCCCCTGGC TATCCAACAA CTACGAACGA 
AGCCGCGCCG TGGCGACACA GAAGCACGCC AACGTGGACG ACCTTCTCGT ATCCACTCCG 
CCGAACGACG GTGATGAGGG GTGTGTGCCT GACAACGCCA ACGACCTCAG CCACGCTCGT 
CAGCGCCAAA GACGAAGCAC ACTGGTGCCG CCACTGCCAC CGCCACCTTC GCCACCGCAA 
GCCTCAACAA CACCGTCAGC GGCACCGACA GACACGGTGT TTGCTGCCAT CCCACGCTCC 
GGTGCGCTTC CCATCCTGTC AGAGCACACC GTAACGGATG CACAGACAGC GGAGGACTTT 
GTGCAGGGGG TAAGAGGAAT CATCAGCTCA CTACAGCAGT ACAGGAACTC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001686206.1
CDS	1..7317
Translation	MGAGLSQQPTPSSKLASAHPSSSAPADAWKSSMRDEVLCGKPLRQSSPETSAAVPRENLYSIVEEEDIDIDYADEESVQACKGSGSGSGTTHPQHRYPVMMPGAGLARVAVPPIPVMSAIAAPASPVSACSSTATDTPRSILRQSGTGGGRTPRSNSRRISFVGVSSPRPDEHRCCRDGDEESGDDSDDYESAEPSLRYDIVTLAMSATSTPPRHSRKVHSHDPSLHNGHYHSHEHGEDDSNGALSPVPASQHQQKRTSPSLSPERPRLSSRSPTHSPPRDSGSGYTISAGAPLSAAVTAPSAPPPPEEETDVITLDAAPQHRALDEQDLLKSPKRGGNQRRAKSPSMGTNGRDEDVLTQPHNRSEPSGSSHGAVVVRRLPPRQRWPSEAYQDEPQTEVSKTQRARRSRRERLDPEADMGSSSSRCSSDMGSSCTGSDDSLSARYASPLKLHQEGYRGSSYQSTSSSPPALACAAAVGSAGAPPIGHPSPHRRSTSARKDRDGTSSERSREKSLTPRSPPSLTQRDDTDEPIRGSSDHDHHHRHSSRGAETRSGLPAEAGAALPTSHNRGRGTRVGVETRGPYTHAVAAAFHLCREASYSPSTITDRSSGQSVAGDLSGSVSEAAAQEARRAPRNYQQQHEVVPSALSATTAAAPLRLSKDRKVKKAVVAEEPQVYVDPLLLERRHRHELTRQESPSHVERHSGLHARRRRPGSRTAAQQQPTTFQSVSLRDIMMRHDSPETKSRRGTPSSQSTAPAILRLPSDVRQLQLQHNGDGDDDDAAHTLTPGAVALEASVASSSLLSSGGVPADELFWDEPTPTSRASSRSPPPVSPRSSSTLAPNTPDRHKVLRKKLVVVVRRKKNGSAQSENDPVVQMSLLPFKEETAALLKANASLQGSPQQRRAHSSGTSAAATVPTLEKLINKVERGESASVFSRCLSRASEHQGQRPRELSERRDGGDDHAGENEISEENRRRFRHALSVLSSRSVSPRASVSPSTTSKAGRSQRSRTRSPDSQINASYACARPLRCSGSASEESVSHTGDAALRRSSGRRHISHVAAVSETAAASIGATSPPQRRSARGGSRHSDEKSARRSEGGHRHKSRRRHSHESIAHSTAGHQRRSLSGASMPGAPYCAAQNDEFYGAADMAKDVATLFPSPFQERIGITPMSMPLLPRLVLRAELGALPQGQYAHHPHSNIDAAQRTNGSWSASDDEDEHAMGRITDSESGGRRSRPLMLQFSSGTQTDRATGVNHAFGSARSGMPLLAATPVQRAAAIGPIGGTLSSAHPGKPVGTSVPTIGAGLAASLLAPTHNAAGDAVFNEEMALAVEKRAANSPLVAEASATESPASFVKRAASPAAAAKPFTDSNSRILSGSGSGAAKGRGEAAVFGGNVGASRAAYAPLFSATSAHVAASSSRPAAQDLSTAARAMMDATTSVPLFFPPLTVDNVSQLSHDWQSTLRERSSKAKRSVMESFLIASAEAGAATTAAAVAAAAPSPPLYLADPAATDVNPSDMCAGGCGGLTVPAVGAEAPSEWQQRARPAQAHQGLAVEEFYGRSVTSAAIANAPRSAHSLPPRSGPQSFSEIKCDVWQRSGSPQLAAQREPEQQQATRSPPFSAPSENKSVQGKSAMPVGSVSKSCAASKSRDGVEERSTSARKHSRRRRRRASPERRANSNGDQLTDALNAETLSTVAADSPCKSSSSAEKINMVGDVTALAGVSPQPLSRESLPVPATLGEAKQKAQQGDQTSDSSSRPCSAVGNGTAANAAVATATDGVGARSAPPPPPPFFSIVEAAAESRASRSVEKAAEKGRGNHQRDERREHLSRHSPSPFSSEASQSPSSKRRKTSSKHKTKHDGDDKHKKRKHGHGRHGRYSRSGTRSLSGQTCDTAAAEIDSDSDVDSNLTTSIEFQLSRLLLLREAAVFTKPPLSAAVDASRRNTRKKDKADGKAARKAAAAKDEHHRHRNRSSKEKRKRSSKRAAKQRSKSPAATTESRLSDGADRKKKKREKAYHCSTSPSKPGPLPPPPPHQRSRALYGEEAAASAAASLSDVASRYRLGAPGCSPRDDGAAFASTRPYFRVGRIYRGGQGEDAEAPYAHHRAAAHTGADSETPYERYRRPSHTSPLRGQRSYVAPTASGWADGFRPACRPAPRPAATAEDPSLWHRSYSSASRPSTRWNDYVRDAERGAAGRSSSSPYMRAHHSRSLGQRRRGSDAGIPTAGECGRGSSAEGGSPSSEQPGSGTYSATYASRRRQTDEFDDINVAPIRQYFNDHGQVGADERGGGVQYGTTSAFERHPSRSAASNRAPTARPWLSNNYERSRAVATQKHANVDDLLVSTPPNDGDEGCVPDNANDLSHARQRQRRSTLVPPLPPPPSPPQASTTPSAAPTDTVFAAIPRSGALPILSEHTVTDAQTAEDFVQGVRGIISSLQQYRNSV

Target ORF information:

RefSeq Version	XM_001686154.2
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001686154.2

ORF Insert Sequence:

ATGGGAGCGG GGCTGTCGCA GCAACCAACA CCCTCGTCGA AGCTCGCCTC GGCGCATCCG 
TCTTCTAGCG CTCCTGCGGA TGCGTGGAAG TCGAGCATGA GGGATGAGGT GCTGTGCGGG 
AAGCCACTGC GGCAGAGCTC GCCGGAAACC TCCGCAGCCG TGCCGCGGGA AAACCTGTAC 
AGCATCGTGG AGGAAGAAGA TATTGATATA GACTACGCAG ATGAAGAGAG TGTGCAGGCC 
TGTAAGGGCA GCGGTAGCGG CAGCGGCACA ACACACCCGC AGCACCGCTA TCCCGTCATG 
ATGCCGGGAG CCGGCTTAGC GAGGGTCGCT GTGCCGCCGA TACCCGTGAT GAGCGCCATC 
GCCGCACCAG CCAGCCCAGT GAGCGCCTGC AGCTCCACCG CCACCGATAC GCCACGGTCT 
ATCTTGAGAC AATCAGGCAC CGGAGGCGGC CGGACGCCGC GGTCGAACAG CCGCCGAATT 
AGCTTCGTGG GAGTCTCGTC TCCCCGCCCG GATGAGCACC GCTGCTGTCG CGATGGCGAT 
GAAGAAAGCG GAGACGACAG TGACGACTAC GAGTCAGCGG AGCCGTCGCT GCGCTACGAC 
ATCGTGACGC TGGCGATGTC TGCCACCTCA ACCCCGCCGC GGCACTCTCG GAAGGTACAC 
AGCCACGATC CTTCTCTGCA CAATGGTCAC TACCACAGCC ACGAACATGG CGAGGATGAC 
AGCAACGGGG CGTTGAGCCC GGTGCCGGCG TCACAGCACC AACAAAAGCG GACCTCCCCG 
TCACTTTCAC CGGAGCGTCC GCGCCTGTCC AGCCGCTCTC CAACCCACTC ACCGCCGCGC 
GACAGTGGAA GCGGATACAC AATTTCGGCC GGGGCGCCAT TATCGGCTGC CGTGACAGCC 
CCCTCCGCGC CTCCTCCGCC TGAGGAAGAG ACGGACGTGA TTACGCTTGA CGCGGCGCCG 
CAGCACCGCG CTCTTGACGA GCAGGATCTC TTGAAGAGCC CCAAACGAGG CGGCAATCAG 
CGTCGTGCGA AAAGCCCTTC GATGGGAACC AACGGACGCG ATGAGGACGT GTTGACCCAG 
CCGCACAATC GCAGCGAACC AAGCGGTAGC AGCCACGGCG CCGTTGTCGT GCGTCGCCTG 
CCTCCGCGGC AGCGCTGGCC ATCAGAGGCG TACCAAGATG AACCCCAAAC AGAGGTGAGC 
AAGACGCAGC GAGCGCGTCG ATCCCGGCGT GAGAGACTCG ACCCAGAGGC CGACATGGGC 
AGCAGCAGTA GCCGCTGCAG CAGCGACATG GGCTCGAGCT GCACTGGCAG CGACGACTCC 
TTGTCAGCAC GCTACGCCTC ACCCCTTAAG CTGCATCAAG AGGGGTATCG AGGGTCGAGC 
TACCAGTCGA CCTCATCATC GCCACCCGCG CTCGCCTGCG CAGCGGCAGT GGGAAGTGCA 
GGGGCACCGC CGATCGGTCA CCCCTCGCCG CATCGACGTA GTACAAGCGC GAGAAAGGAT 
CGTGATGGCA CTAGCAGCGA AAGAAGTCGA GAAAAGTCGT TGACGCCGCG GTCACCTCCG 
TCGCTGACAC AGCGAGACGA CACAGACGAG CCGATCCGCG GCAGCAGCGA CCACGATCAC 
CATCATAGAC ACTCTTCCAG AGGAGCGGAG ACAAGGTCTG GCCTGCCAGC AGAGGCTGGA 
GCAGCCCTAC CGACATCGCA CAACCGCGGT CGCGGCACGC GAGTGGGTGT GGAAACGCGA 
GGTCCGTACA CCCACGCAGT TGCCGCTGCC TTCCATCTGT GCAGAGAAGC GAGTTACTCT 
CCTTCGACCA TCACAGACCG CAGCTCCGGC CAGAGCGTGG CAGGCGACCT GTCTGGCTCC 
GTGTCCGAGG CGGCCGCGCA GGAGGCGAGA CGGGCACCGA GGAACTACCA GCAGCAGCAT 
GAGGTGGTAC CGTCAGCTCT ATCAGCTACG ACGGCGGCGG CGCCACTGAG GCTATCCAAG 
GATAGGAAGG TCAAAAAAGC AGTGGTTGCC GAAGAGCCGC AGGTGTACGT GGATCCGCTC 
TTGCTCGAGC GTCGACATCG CCACGAGCTC ACGCGGCAAG AGTCGCCGTC GCATGTAGAG 
CGGCACTCGG GGCTCCACGC GCGCCGCAGG CGCCCGGGGT CTAGAACTGC GGCGCAGCAG 
CAGCCAACCA CCTTCCAGTC AGTGAGCTTG CGGGACATAA TGATGCGCCA CGACTCGCCG 
GAGACCAAAT CACGACGCGG CACGCCATCC AGTCAGTCCA CTGCTCCGGC GATATTACGG 
CTTCCTTCTG ACGTGCGGCA GCTTCAGCTC CAACACAACG GGGATGGCGA CGATGATGAT 
GCAGCTCACA CCCTTACACC CGGCGCGGTA GCCTTGGAAG CATCTGTTGC GTCCTCTTCG 
CTCCTCTCTA GTGGTGGCGT GCCGGCCGAT GAGCTTTTCT GGGACGAGCC GACGCCAACT 
TCGCGCGCCA GCAGCCGTTC ACCGCCCCCT GTTTCCCCAA GATCCTCCTC AACGCTGGCA 
CCCAACACTC CCGACAGACA CAAAGTGCTT CGCAAGAAGC TTGTTGTCGT CGTGCGCCGA 
AAGAAGAATG GCTCAGCACA GTCAGAGAAC GATCCTGTGG TACAGATGTC GCTGCTGCCC 
TTCAAGGAGG AAACTGCAGC TCTACTGAAA GCGAACGCCT CGCTGCAAGG GTCACCGCAG 
CAGCGACGCG CGCACAGCTC CGGCACCTCT GCCGCGGCAA CCGTTCCGAC TCTGGAGAAG 
CTGATCAACA AAGTGGAGCG AGGCGAGAGT GCCTCCGTGT TCTCCCGCTG CCTCAGCCGC 
GCATCTGAAC ATCAAGGCCA GAGGCCGCGT GAACTTTCGG AGAGGCGCGA CGGAGGCGAC 
GACCACGCCG GTGAAAACGA GATCAGCGAG GAAAACCGTA GGCGGTTCCG GCATGCGCTG 
TCAGTGTTGT CATCTCGAAG TGTGAGCCCG CGCGCGTCTG TCTCACCATC GACTACGTCG 
AAGGCGGGGC GGTCTCAACG AAGTCGCACA CGCTCACCAG ACTCACAAAT CAATGCCTCA 
TACGCTTGCG CGCGACCACT TCGCTGCAGC GGCAGCGCTA GCGAGGAGTC CGTATCGCAC 
ACTGGCGATG CCGCGCTGAG AAGGAGCAGT GGCCGCAGGC ATATATCTCA CGTGGCGGCG 
GTGAGCGAGA CGGCAGCGGC GAGCATAGGT GCGACATCGC CGCCCCAGCG TCGATCTGCC 
CGTGGTGGCT CACGTCACTC CGACGAGAAG TCGGCGCGTA GGTCCGAGGG TGGGCATCGC 
CACAAGTCGC GGCGGAGACA TAGTCACGAG AGCATAGCAC ACAGCACCGC TGGGCACCAG 
CGGCGTTCTC TTTCAGGCGC ATCCATGCCT GGCGCACCGT ATTGTGCGGC GCAGAACGAC 
GAGTTCTACG GCGCCGCGGA CATGGCAAAG GATGTGGCGA CGCTCTTTCC ATCACCTTTT 
CAGGAGCGCA TCGGCATCAC ACCGATGTCA ATGCCGCTTC TACCGCGGCT GGTGCTGCGA 
GCGGAGCTTG GTGCGCTGCC GCAAGGGCAA TATGCCCATC ACCCGCACAG TAACATCGAC 
GCAGCTCAGC GCACCAACGG TTCGTGGTCC GCCTCCGATG ACGAGGATGA GCATGCCATG 
GGCCGAATCA CCGATAGCGA AAGCGGTGGG CGTCGTTCAC GTCCCCTGAT GCTGCAGTTC 
AGCAGTGGCA CGCAAACGGA TCGGGCGACA GGAGTCAACC ATGCTTTTGG TAGCGCTCGC 
AGTGGGATGC CTCTCTTAGC GGCGACGCCA GTGCAGAGGG CTGCCGCCAT TGGGCCGATC 
GGAGGCACGC TTTCCTCAGC TCACCCCGGC AAACCCGTTG GCACGTCAGT GCCAACAATC 
GGCGCTGGTC TGGCGGCATC CTTACTCGCA CCAACGCACA ATGCAGCTGG CGATGCCGTG 
TTCAACGAAG AGATGGCGTT AGCTGTCGAA AAGCGTGCAG CGAACTCGCC GCTCGTCGCG 
GAGGCTTCCG CTACAGAGTC GCCAGCATCG TTTGTAAAGC GGGCGGCGTC ACCGGCAGCG 
GCAGCGAAGC CCTTCACCGA CTCCAACTCG CGTATTCTCA GCGGCAGTGG CAGCGGCGCT 
GCTAAAGGAA GAGGTGAGGC CGCGGTCTTT GGCGGCAATG TCGGGGCATC GAGGGCGGCG 
TACGCCCCTC TATTTTCGGC GACCTCAGCG CACGTGGCCG CGTCCAGCTC TAGGCCGGCC 
GCCCAGGACC TTAGCACCGC AGCACGTGCA ATGATGGATG CCACCACCAG TGTGCCGCTC 
TTTTTTCCGC CGCTGACTGT CGACAACGTG TCACAGTTAT CTCACGACTG GCAGTCCACG 
CTGCGTGAGC GCTCGAGCAA GGCGAAGCGG AGTGTGATGG AGAGCTTTCT GATTGCGTCG 
GCCGAGGCTG GTGCGGCGAC AACGGCAGCT GCTGTCGCTG CTGCTGCACC GTCACCACCG 
CTGTACCTCG CGGACCCCGC CGCCACTGAC GTAAACCCTT CGGACATGTG CGCTGGTGGT 
TGCGGAGGCC TTACCGTCCC TGCTGTTGGT GCTGAGGCTC CCTCTGAGTG GCAGCAACGA 
GCCAGACCAG CACAGGCACA CCAGGGTCTT GCGGTAGAGG AATTTTATGG GCGTTCTGTC 
ACCTCAGCTG CCATTGCCAA TGCGCCGCGC TCCGCGCATT CCCTCCCGCC ACGATCTGGA 
CCCCAGTCTT TCTCGGAAAT CAAGTGCGAC GTGTGGCAAC GCAGTGGCAG CCCACAATTG 
GCGGCGCAGC GGGAGCCTGA GCAGCAGCAG GCAACGAGGT CTCCACCGTT TTCAGCACCC 
TCGGAAAACA AGTCCGTGCA GGGCAAATCT GCAATGCCGG TAGGCAGTGT TTCGAAAAGC 
TGTGCAGCCT CCAAGAGCAG AGACGGCGTA GAGGAACGTT CTACGTCAGC ACGCAAGCAC 
AGTCGCCGTC GACGTCGCCG CGCTTCGCCG GAACGTCGCG CCAACAGCAA TGGCGACCAG 
CTCACAGACG CCCTTAATGC GGAGACCCTG TCGACAGTGG CAGCCGACTC GCCATGCAAG 
AGCAGCAGCA GCGCTGAAAA GATCAACATG GTTGGCGACG TCACTGCGCT GGCGGGGGTT 
TCTCCACAAC CTCTGTCGAG GGAGTCTCTC CCCGTGCCTG CCACACTTGG CGAAGCGAAG 
CAGAAGGCCC AGCAGGGTGA CCAGACGAGC GACAGCAGCA GCCGACCGTG TTCTGCAGTA 
GGAAACGGAA CGGCGGCCAA CGCAGCTGTC GCCACCGCTA CCGACGGTGT GGGTGCCCGC 
TCTGCGCCTC CGCCGCCGCC GCCTTTCTTC TCCATCGTTG AGGCTGCCGC GGAGTCACGC 
GCGTCGCGCT CGGTGGAGAA GGCAGCGGAA AAGGGCCGCG GTAATCATCA ACGAGACGAG 
CGTCGCGAAC ACCTCAGCCG TCACTCTCCC TCGCCCTTCT CCTCGGAGGC ATCGCAGTCG 
CCGAGCAGCA AGCGACGGAA AACGTCGAGT AAGCACAAGA CAAAGCACGA CGGCGACGAC 
AAGCATAAGA AGCGGAAGCA TGGCCATGGG CGTCATGGCC GTTACAGTCG CAGTGGCACG 
CGCTCGCTAT CGGGTCAGAC CTGTGACACC GCCGCCGCTG AAATTGACAG TGACAGCGAC 
GTGGACAGCA ATCTCACGAC ATCGATAGAG TTCCAGCTGT CTCGGCTGCT ACTGCTGCGC 
GAGGCGGCAG TGTTCACGAA GCCGCCTCTC TCTGCGGCCG TTGACGCGTC AAGGCGAAAC 
ACGCGCAAGA AAGACAAGGC AGATGGAAAG GCAGCAAGAA AGGCTGCTGC AGCGAAGGAC 
GAGCACCACC GCCATCGCAA CCGGAGTTCA AAGGAGAAGA GAAAGAGGTC ATCGAAGAGG 
GCCGCTAAGC AGCGCAGCAA GTCGCCTGCG GCCACCACCG AGTCGCGGCT CAGCGACGGC 
GCGGACAGGA AGAAAAAAAA GAGGGAAAAA GCATACCACT GCTCAACCTC TCCGTCAAAG 
CCGGGCCCTC TGCCGCCACC GCCCCCGCAC CAGCGTTCGC GCGCCTTGTA CGGTGAGGAG 
GCCGCGGCAA GCGCTGCAGC GTCGCTTTCG GACGTGGCCT CCCGCTACCG CCTCGGCGCC 
CCTGGCTGCA GTCCCCGCGA CGACGGCGCC GCTTTCGCAT CGACGCGCCC CTACTTCAGG 
GTCGGCAGGA TCTACCGCGG AGGTCAGGGA GAGGACGCGG AGGCGCCTTA TGCACATCAT 
CGCGCCGCAG CCCACACTGG AGCAGACTCG GAAACCCCGT ATGAACGCTA TCGTCGGCCC 
TCTCACACCT CGCCACTGCG CGGCCAACGC TCCTACGTGG CACCGACAGC ATCGGGATGG 
GCAGACGGTT TTCGCCCAGC CTGCAGACCC GCGCCACGCC CTGCCGCCAC CGCCGAGGAT 
CCCTCCTTGT GGCATCGCAG CTACAGCAGC GCAAGCCGTC CCTCGACCCG CTGGAATGAC 
TACGTTCGAG ATGCAGAGCG CGGCGCCGCT GGCAGGTCGT CGTCCTCTCC GTACATGCGC 
GCTCATCACT CCCGGTCCCT TGGTCAGCGA CGACGCGGTA GTGACGCTGG CATCCCTACG 
GCAGGGGAGT GTGGTCGCGG GAGTAGTGCC GAGGGAGGCT CGCCCAGCTC AGAACAACCG 
GGCTCCGGCA CCTACTCTGC CACCTACGCA AGCCGTCGTC GGCAGACGGA TGAGTTTGAC 
GATATCAACG TCGCGCCGAT ACGACAGTAC TTCAACGACC ATGGCCAAGT CGGCGCCGAC 
GAGCGCGGCG GCGGTGTGCA GTACGGCACC ACCTCAGCCT TTGAGCGGCA TCCCAGTCGG 
TCAGCCGCCT CCAACCGCGC ACCGACGGCC CGCCCCTGGC TATCCAACAA CTACGAACGA 
AGCCGCGCCG TGGCGACACA GAAGCACGCC AACGTGGACG ACCTTCTCGT ATCCACTCCG 
CCGAACGACG GTGATGAGGG GTGTGTGCCT GACAACGCCA ACGACCTCAG CCACGCTCGT 
CAGCGCCAAA GACGAAGCAC ACTGGTGCCG CCACTGCCAC CGCCACCTTC GCCACCGCAA 
GCCTCAACAA CACCGTCAGC GGCACCGACA GACACGGTGT TTGCTGCCAT CCCACGCTCC 
GGTGCGCTTC CCATCCTGTC AGAGCACACC GTAACGGATG CACAGACAGC GGAGGACTTT 
GTGCAGGGGG TAAGAGGAAT CATCAGCTCA CTACAGCAGT ACAGGAACTC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_34_1130 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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