ORF » Species Summary » Leishmania major strain Friedlin » LMJF_18_1380 cDNA ORF clone

LMJF_18_1380 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_18_1380 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_18_1380 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb02115 XM_001682495.1
Latest version!		 Leishmania major strain Friedlin putative pyruvate dehydrogenase E1 component alpha subunit partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_18_1380 ( XM_001682495.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001682547.1 Leishmania major strain Friedlin putative pyruvate dehydrogenase E1 component alpha subunit partial mRNA.
    Price & Availability↑
    CloneID OLb02115
    Clone ID Related Accession (Same CDS sequence) XM_001682495.1
    Accession Version XM_001682495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative pyruvate dehydrogenase E1 component alpha subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007259). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTCAAGT GCGCGACTCG CTGCCTTCTG GACACCAAGA CGGTGCCGCT GAAGCCGCAG 
    CGCCCCTTCA AGCTGCACAC GGCTGGCCGC ACCGACATGG CACCCCTGCC GACGCAGGCG 
    GTATACGACG CAGAGCAGCT GAAGCAGAGC CTTGCCCTGA TGTTCCGCAT CCGCCGCATG 
    GAGTCTCTGT GCGATCAGTC CTACAAGCTA AAGAAGATTC GCGGCTTCTG CCACCTCTAC 
    ATCGGGCAGG AGGCCATCCC GGCTGGCATG GAGAACGTTC TGACCTTCGA GGACCCCATT 
    ATCACTGGCT ACCGTGATCA CGGCTGGTAC ATTTCTCGCG GTGGCAAGCC CGAGGATGTC 
    TTCGCTGAAA TGTTCGGTCG TCAGGGCGGC TGCAGCAAGG GCAAGGGTGG CTCGATGCAC 
    ATGTACAGGG TGGATAACGG CTTCTACGGC GGTAATGGTA TCGTCGGTGC GCAGGTCTCC 
    ATCGGCGCTG GCCTTGCCTG GCGCTTCGCG ATGGAGAACC GCGACAGCCC GAAGCACGTC 
    GCGGTCACCT TCTACGGCGA CGGTGCTGCC AACCAGGGCC AGATCTACGA GTCCATGAAC 
    ATTGCCGCTC TGCAGCGCCT CCCCGTCATC TTCGCCGTGG AGAACAACCA CTTCGGTATG 
    GGCACTAGCG CCGCTCGCGG CTCCTATCAG GCGGAGTTTT ACCGCCGTGG TGACTACATC 
    CCTGGTATCA AGGTCGATGG CATGGACGTG CTCGCGGTGC AGGAGGGCAC CCGCTACGCA 
    CGCGACTACT GCATGTCGGG CAAAGGCCCG ATTGTGATGG AGCTGGACTG CTACCGCTAC 
    ATGGGCCATA GCATGTCAGA CCCCGACAAC CAGTACCGCA CCAAGAGCGA CATCCAGCAC 
    GTGAAGCAGG AGCGTGACTG CATTCGCAAG ATGCGCGAGT TCATGGCAAC GGAGGGCATC 
    ATGACCGAGG ACGAGATGAG CAAGATGGAG AAAGACGTAA AGAAGGAGGT GGACCAGGAC 
    CTGCAGAAGG CCCAGAAACA GCCTATGACA AAGCTGGATG AGCTCTTCAC GGACATCTAC 
    GTGGGTGAGC AGTACGAGCA CCGCACTTGC CAGGGCACTG TGTACCACAA GCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001682547.1
    CDS1..1137
    Translation

    Target ORF information:

    RefSeq Version XM_001682495.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative pyruvate dehydrogenase E1 component alpha subunit partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001682495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTCAAGT GCGCGACTCG CTGCCTTCTG GACACCAAGA CGGTGCCGCT GAAGCCGCAG 
    CGCCCCTTCA AGCTGCACAC GGCTGGCCGC ACCGACATGG CACCCCTGCC GACGCAGGCG 
    GTATACGACG CAGAGCAGCT GAAGCAGAGC CTTGCCCTGA TGTTCCGCAT CCGCCGCATG 
    GAGTCTCTGT GCGATCAGTC CTACAAGCTA AAGAAGATTC GCGGCTTCTG CCACCTCTAC 
    ATCGGGCAGG AGGCCATCCC GGCTGGCATG GAGAACGTTC TGACCTTCGA GGACCCCATT 
    ATCACTGGCT ACCGTGATCA CGGCTGGTAC ATTTCTCGCG GTGGCAAGCC CGAGGATGTC 
    TTCGCTGAAA TGTTCGGTCG TCAGGGCGGC TGCAGCAAGG GCAAGGGTGG CTCGATGCAC 
    ATGTACAGGG TGGATAACGG CTTCTACGGC GGTAATGGTA TCGTCGGTGC GCAGGTCTCC 
    ATCGGCGCTG GCCTTGCCTG GCGCTTCGCG ATGGAGAACC GCGACAGCCC GAAGCACGTC 
    GCGGTCACCT TCTACGGCGA CGGTGCTGCC AACCAGGGCC AGATCTACGA GTCCATGAAC 
    ATTGCCGCTC TGCAGCGCCT CCCCGTCATC TTCGCCGTGG AGAACAACCA CTTCGGTATG 
    GGCACTAGCG CCGCTCGCGG CTCCTATCAG GCGGAGTTTT ACCGCCGTGG TGACTACATC 
    CCTGGTATCA AGGTCGATGG CATGGACGTG CTCGCGGTGC AGGAGGGCAC CCGCTACGCA 
    CGCGACTACT GCATGTCGGG CAAAGGCCCG ATTGTGATGG AGCTGGACTG CTACCGCTAC 
    ATGGGCCATA GCATGTCAGA CCCCGACAAC CAGTACCGCA CCAAGAGCGA CATCCAGCAC 
    GTGAAGCAGG AGCGTGACTG CATTCGCAAG ATGCGCGAGT TCATGGCAAC GGAGGGCATC 
    ATGACCGAGG ACGAGATGAG CAAGATGGAG AAAGACGTAA AGAAGGAGGT GGACCAGGAC 
    CTGCAGAAGG CCCAGAAACA GCCTATGACA AAGCTGGATG AGCTCTTCAC GGACATCTAC 
    GTGGGTGAGC AGTACGAGCA CCGCACTTGC CAGGGCACTG TGTACCACAA GCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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