ORF » Species Summary » Leishmania major strain Friedlin » LMJF_10_0420 cDNA ORF clone

LMJF_10_0420 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_10_0420 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_10_0420 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb00828 XM_001681320.1
Latest version!		 Leishmania major strain Friedlin dihydroxyacetone kinase 1-like protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_10_0420 ( XM_001681320.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001681372.1 Leishmania major strain Friedlin dihydroxyacetone kinase 1-like protein partial mRNA.
    Price & Availability↑
    CloneID OLb00828
    Clone ID Related Accession (Same CDS sequence) XM_001681320.1
    Accession Version XM_001681320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product dihydroxyacetone kinase 1-like protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007251). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCGCCG TGGTGCCGAA GTTTATCGAC GAGCCCAGCA GCGTCGTGGC GGTTGCTGTG 
    GACGGCCTCT GTGAGCTGAA CAACCATGTG AAGAAGATAG AGAACACCAA CGTTGTCGTC 
    TCGTGTTGTC TAGATCCGAC CAAGGTCCTG CTGCTCTGTG GCGGCGGCTC CGGCCACGAA 
    CCGGCTCACT CCGGGTTCGT CGCTGAGGGC TGGCTGAGCG CGGCGGTATG CGGCAGCGTC 
    TTCGCCTCCC CGCCCACGGC GCACGTGAGC GCCGGCATAG AGTACCTGGC CAAGCGCCAG 
    GGACCTGATG GTCCAGGCAT CCTCGTCATC ATCAAAAACT ACGCTGGTGA CATTCTCAAC 
    TTCGAGTACG CCGTCTGTCA AGCCCGTGCA GAGGGAATTC AAGTCGAAAC AGTGCTGGCG 
    GCCGATGATG CAGTGTTCGG CACTAAAGAT ATATCGAAGC GTCGCGGTGT GGCGGGGTGC 
    TGCCTCCTCT ACAAAATCCT CGGCGCTGCT GCAGCGCGTG GTTGGTCGCT GACGCAGCTG 
    AAGGCGCTCG CTGACCGCGT CTCACGCAAC ATGCGATCCA TCGGTGCCTC GCTCTCGTCC 
    TGCTCGCTGC CGGGCAACCC GGCCTCGTCG GTGATACCCT CCGGAACGGT TGAGGTGGGG 
    CTGGGCATCC ATGGAGAGAA GGGACTCTTC CAGATCCCCT TCCAAGGCGC AGCGTCCCTC 
    GTCCACTTCC TCGTTGGGAT TGTGACGGGC AAGGGAGAAG CAGGTGCTTC CGGCAAGACC 
    ACCGCCATCC ACTCCAGTGT CAAGGCTCTC TTGCTGGTGA ACAACCTGGG TGGGACAACG 
    GACCTGGAGA TGTCTACCCT TGCGCACCAC GCCTTGCGCG AACTTGCTGC CGCGCAGCTG 
    ACTGTTGTTG GAGTGCACAG CGGTCGCCAC ATGACATCGT TGGACATGCA CGGCTTCTCG 
    CTGACGCTGC TCATAGTCGA GGACGAGAGG GACCTGGAGT TTATGCTCAA TACGAACGCG 
    TTGCAGAAGC CGCTCATGAA CTTCCACGCG CCGCAGTTGA GCGGTGCGAC GGTTCCGGGG 
    CCGCTGACTG CGCTGCAGCT GGCACGGCAA GAGGCGGAGG CAGCAGGGCG CGCAGCAGCC 
    ACGCCCACCG GCAGCCCCCT CTGCGCCGCT GTGCAGCGCG TCTTCGAGAA GCTCATGACG 
    ATGGAGGCCT ACTTCAACGG CCTGGACGCG GAGGTAGGCG ACGGCGACCT CGGCAGCGGC 
    GTGCATCGCT CGGCAATTGC GGTGCTGGAA ATGCTGCCTT ACCTACCGTG GGAGGCCGAC 
    ATGCGGCGGA CCTTCACGCT CATCTCCAAA GCCGTCGCCG ACGCCTTCGC CGGCACCTCT 
    GGCCCGCTCT ACGGTGCGCT GCTCATCGGC GGCGGCAACG CCGCGGTGCA GGCGCTGAAG 
    AGCGGTGGCG CAGTTGAGGC GGTGCGCGCG GCACTCGCGC ACGGCAGCCA CAATGTGCAG 
    GAGCTCAGCG GCGCGCGTCG GGGCGATCGC ACGATGGTGG ACGTGCTGGA GGGCATGCGC 
    GTGTGCCCGG CCGTCGCAGC GGCAGCGACG ACGCCGGAGC TGCTCAAGGC GTGCCACGAA 
    GCGGCGCGCG CGGCCGCCGA CGCGACGGCG ATGCTTCCCG CCCAGCTCGG CCGCAGTCGC 
    TACATGGAGG GAAAGGAGAT CGGCAAGAAG GACCCGGGCG CGGAGCTCGT GGTGGCCTGG 
    GTCGAAGCGC TTGCCTCGGA GAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001681372.1
    CDS1..1770
    Translation

    Target ORF information:

    RefSeq Version XM_001681320.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin dihydroxyacetone kinase 1-like protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001681320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCGCCG TGGTGCCGAA GTTTATCGAC GAGCCCAGCA GCGTCGTGGC GGTTGCTGTG 
    GACGGCCTCT GTGAGCTGAA CAACCATGTG AAGAAGATAG AGAACACCAA CGTTGTCGTC 
    TCGTGTTGTC TAGATCCGAC CAAGGTCCTG CTGCTCTGTG GCGGCGGCTC CGGCCACGAA 
    CCGGCTCACT CCGGGTTCGT CGCTGAGGGC TGGCTGAGCG CGGCGGTATG CGGCAGCGTC 
    TTCGCCTCCC CGCCCACGGC GCACGTGAGC GCCGGCATAG AGTACCTGGC CAAGCGCCAG 
    GGACCTGATG GTCCAGGCAT CCTCGTCATC ATCAAAAACT ACGCTGGTGA CATTCTCAAC 
    TTCGAGTACG CCGTCTGTCA AGCCCGTGCA GAGGGAATTC AAGTCGAAAC AGTGCTGGCG 
    GCCGATGATG CAGTGTTCGG CACTAAAGAT ATATCGAAGC GTCGCGGTGT GGCGGGGTGC 
    TGCCTCCTCT ACAAAATCCT CGGCGCTGCT GCAGCGCGTG GTTGGTCGCT GACGCAGCTG 
    AAGGCGCTCG CTGACCGCGT CTCACGCAAC ATGCGATCCA TCGGTGCCTC GCTCTCGTCC 
    TGCTCGCTGC CGGGCAACCC GGCCTCGTCG GTGATACCCT CCGGAACGGT TGAGGTGGGG 
    CTGGGCATCC ATGGAGAGAA GGGACTCTTC CAGATCCCCT TCCAAGGCGC AGCGTCCCTC 
    GTCCACTTCC TCGTTGGGAT TGTGACGGGC AAGGGAGAAG CAGGTGCTTC CGGCAAGACC 
    ACCGCCATCC ACTCCAGTGT CAAGGCTCTC TTGCTGGTGA ACAACCTGGG TGGGACAACG 
    GACCTGGAGA TGTCTACCCT TGCGCACCAC GCCTTGCGCG AACTTGCTGC CGCGCAGCTG 
    ACTGTTGTTG GAGTGCACAG CGGTCGCCAC ATGACATCGT TGGACATGCA CGGCTTCTCG 
    CTGACGCTGC TCATAGTCGA GGACGAGAGG GACCTGGAGT TTATGCTCAA TACGAACGCG 
    TTGCAGAAGC CGCTCATGAA CTTCCACGCG CCGCAGTTGA GCGGTGCGAC GGTTCCGGGG 
    CCGCTGACTG CGCTGCAGCT GGCACGGCAA GAGGCGGAGG CAGCAGGGCG CGCAGCAGCC 
    ACGCCCACCG GCAGCCCCCT CTGCGCCGCT GTGCAGCGCG TCTTCGAGAA GCTCATGACG 
    ATGGAGGCCT ACTTCAACGG CCTGGACGCG GAGGTAGGCG ACGGCGACCT CGGCAGCGGC 
    GTGCATCGCT CGGCAATTGC GGTGCTGGAA ATGCTGCCTT ACCTACCGTG GGAGGCCGAC 
    ATGCGGCGGA CCTTCACGCT CATCTCCAAA GCCGTCGCCG ACGCCTTCGC CGGCACCTCT 
    GGCCCGCTCT ACGGTGCGCT GCTCATCGGC GGCGGCAACG CCGCGGTGCA GGCGCTGAAG 
    AGCGGTGGCG CAGTTGAGGC GGTGCGCGCG GCACTCGCGC ACGGCAGCCA CAATGTGCAG 
    GAGCTCAGCG GCGCGCGTCG GGGCGATCGC ACGATGGTGG ACGTGCTGGA GGGCATGCGC 
    GTGTGCCCGG CCGTCGCAGC GGCAGCGACG ACGCCGGAGC TGCTCAAGGC GTGCCACGAA 
    GCGGCGCGCG CGGCCGCCGA CGCGACGGCG ATGCTTCCCG CCCAGCTCGG CCGCAGTCGC 
    TACATGGAGG GAAAGGAGAT CGGCAAGAAG GACCCGGGCG CGGAGCTCGT GGTGGCCTGG 
    GTCGAAGCGC TTGCCTCGGA GAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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