ORF » Species Summary » Leishmania major strain Friedlin » TRYS cDNA ORF clone

TRYS cDNA ORF clone, Leishmania major strain Friedlin

The following TRYS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRYS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb07918 XM_003721946.1
Latest version!		 Leishmania major strain Friedlin putative trypanothione synthetase (TRYS), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TRYS ( XM_003721946.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_003721994.1 Leishmania major strain Friedlin putative trypanothione synthetase (TRYS), partial mRNA.
    Price & Availability↑
    CloneID OLb07918
    Clone ID Related Accession (Same CDS sequence) XM_003721946.1
    Accession Version XM_003721946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative trypanothione synthetase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007268). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCATCTC TGCAGCGCGC GTCTGTGAGC TTTAATAAGC CGGGCCACAT TCCGTTTGGT 
    GCTGTGCAGG GATACGCCCC GGGTGGCGTC CCGGCGTACA GCAACAAGCA CGATCACTAC 
    TTCTCCGGCG AGCGCAACAT TGAGGACAAC ATCTTCTTCG GCTTCAAGTA CCAGTGCGTC 
    GAATTTGCGC GCCGCTGGCT ACTGGTGCGT AAGGGACTGT TGCTGCCCGA CGTGAACTGG 
    GCCTGCCACA TTTTCCAATT GAAGGAGGTG CGGGACGCCG CCACGACGGA GAGCTTCGCG 
    GTATTGCAAG TGCGCAACGG TACTACGACG AAGCCAGAGG CCGACGCGCT TCTTGTCTAC 
    CCCTCCACCG ATGCAAATCC AGTTGGTCAT GTCGGCACCA TCACCGAGGT GGGTGACGAC 
    TACGTATGTG TCGCGGATCA GAACTACCGC TTCCACAAGT GGGAGTCCTC CTGCGCGTAC 
    AAGCTGAAGC TTGATCACAG GGACGGCATC TGGACCATCA TCGATGACAT TGACGCGGAC 
    GAAATCGAGA TCCCACTTGG GTGGTTGACG TTTCCTGGCC GCGCAAATCG ACCGGAAGGG 
    GCGCCGCCGG TGGCGCTGCA TCCGTCACTG CACTTCAAGG AGCCGCCGAA GCCGTATCTG 
    CTGCGCCGTA ACTTTCTGCC GACGGAGTCC AAGGCGAACT GGCTCGACAT GAACAACCCG 
    GCGGAGCGGC TATTTGTGGA GGAGTTTGGC ATGGACGTCA GCCGTACCCG CCTGGAGGAG 
    AAGGTCGTCA GCTACTACGA GTCGAATCAC GAGTTTCACC TGCGGTGCGT CGCGTACGGA 
    ACGCAGCTGC ACGCCATCTT CATGGAGGCA ACCGCGCAGG TGATCGAGAG CGATGAGAAG 
    CTCCGGCTCT TTGCCATTCC TGAGGAGTTC TGGCCCCGCA TCCGCCACTC GTGGAAGTAC 
    CAGCAGACGT ACATTTCTGG TCGCTTCGAC TTCGCCTTCA ACAACGAGAC GGGCGAGGTC 
    AAGTGCTTCG AGTACAATGC TGACAGCGCG TCGACGCTGC TAGAGTGCGG CCTCATTCAG 
    CAAAAGTGGG CCGAGTCGGT GGGGCTGGAC AAGCAGGACA CGCGCGGCTC CGGCTTCGCG 
    GTGGAGCGCA ACCTCAAGAT GGCGTGGGCA AACAGCGGGG CGACTGGCCG CGTTCACTTC 
    TGCGTTGATG AGGAAAGGGA AGAGCAGTAC ACGGCCCTCT ACTGCATGCA GGCCGCTGAG 
    GCAGTCGGGT TGGAGGGCAA GCTGTGCATC TTGTTCGATG AGTTCCGCTT TGATGATAAT 
    GGTCACGTTG TCGATAGCGA CGGCGTGCGT GTGCGCAATG TGTGGAAGAC GTGGATGTGG 
    GAGTCGGCCA TCACGGACTA TTACGCCGCC CGGGAGGAGC GAGGCGAGAA CTGGAAGCCG 
    TCCCCGAAGG ACAAGGTGCG ATTGTGTGAC TTGCTCCTCG GCGATGACTG GGAAATTCTC 
    TACTTCGAGC CGATGTGGAA AGTCATCCCG AGCAACAAGG CCATCCTGCC CATGATCTAC 
    CACAACCACC CCGAACACCC AGCCATCTTG AAGGCGGAGT ACGAGTTGAC GGACGAGCTG 
    CGCAAGCACG GGTACGCCAA GAAGCCGATT GTCGGCCGCG TGGGCAGCAA CGTTATCATC 
    ACCTCCGGCG ACGGCGTGGT GCACGCTGAG TCTGGTGGCA AATATGGCAA GCGCAACATG 
    ATTTACCAAC AGCTGTTTGA GCTGAAGAAG CAGGATGACT ACTACGCCAT CATCGGCGGC 
    TGGATGATCG GCGACGCCTT CAGCGGCACC GGCATCCGTG AGGACAAGTC AGTCATCACC 
    GGCGTGGACA GCCCCTTCGC TGCCGTTCGC ATCAAGACCG ACAAACTTCC GCACCCCGTG 
    ACGCTCAAGG ATATCGACAA GATGGCCGAG GACGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003721994.1
    CDS1..1959
    Translation

    Target ORF information:

    RefSeq Version XM_003721946.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative trypanothione synthetase (TRYS), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003721946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCATCTC TGCAGCGCGC GTCTGTGAGC TTTAATAAGC CGGGCCACAT TCCGTTTGGT 
    GCTGTGCAGG GATACGCCCC GGGTGGCGTC CCGGCGTACA GCAACAAGCA CGATCACTAC 
    TTCTCCGGCG AGCGCAACAT TGAGGACAAC ATCTTCTTCG GCTTCAAGTA CCAGTGCGTC 
    GAATTTGCGC GCCGCTGGCT ACTGGTGCGT AAGGGACTGT TGCTGCCCGA CGTGAACTGG 
    GCCTGCCACA TTTTCCAATT GAAGGAGGTG CGGGACGCCG CCACGACGGA GAGCTTCGCG 
    GTATTGCAAG TGCGCAACGG TACTACGACG AAGCCAGAGG CCGACGCGCT TCTTGTCTAC 
    CCCTCCACCG ATGCAAATCC AGTTGGTCAT GTCGGCACCA TCACCGAGGT GGGTGACGAC 
    TACGTATGTG TCGCGGATCA GAACTACCGC TTCCACAAGT GGGAGTCCTC CTGCGCGTAC 
    AAGCTGAAGC TTGATCACAG GGACGGCATC TGGACCATCA TCGATGACAT TGACGCGGAC 
    GAAATCGAGA TCCCACTTGG GTGGTTGACG TTTCCTGGCC GCGCAAATCG ACCGGAAGGG 
    GCGCCGCCGG TGGCGCTGCA TCCGTCACTG CACTTCAAGG AGCCGCCGAA GCCGTATCTG 
    CTGCGCCGTA ACTTTCTGCC GACGGAGTCC AAGGCGAACT GGCTCGACAT GAACAACCCG 
    GCGGAGCGGC TATTTGTGGA GGAGTTTGGC ATGGACGTCA GCCGTACCCG CCTGGAGGAG 
    AAGGTCGTCA GCTACTACGA GTCGAATCAC GAGTTTCACC TGCGGTGCGT CGCGTACGGA 
    ACGCAGCTGC ACGCCATCTT CATGGAGGCA ACCGCGCAGG TGATCGAGAG CGATGAGAAG 
    CTCCGGCTCT TTGCCATTCC TGAGGAGTTC TGGCCCCGCA TCCGCCACTC GTGGAAGTAC 
    CAGCAGACGT ACATTTCTGG TCGCTTCGAC TTCGCCTTCA ACAACGAGAC GGGCGAGGTC 
    AAGTGCTTCG AGTACAATGC TGACAGCGCG TCGACGCTGC TAGAGTGCGG CCTCATTCAG 
    CAAAAGTGGG CCGAGTCGGT GGGGCTGGAC AAGCAGGACA CGCGCGGCTC CGGCTTCGCG 
    GTGGAGCGCA ACCTCAAGAT GGCGTGGGCA AACAGCGGGG CGACTGGCCG CGTTCACTTC 
    TGCGTTGATG AGGAAAGGGA AGAGCAGTAC ACGGCCCTCT ACTGCATGCA GGCCGCTGAG 
    GCAGTCGGGT TGGAGGGCAA GCTGTGCATC TTGTTCGATG AGTTCCGCTT TGATGATAAT 
    GGTCACGTTG TCGATAGCGA CGGCGTGCGT GTGCGCAATG TGTGGAAGAC GTGGATGTGG 
    GAGTCGGCCA TCACGGACTA TTACGCCGCC CGGGAGGAGC GAGGCGAGAA CTGGAAGCCG 
    TCCCCGAAGG ACAAGGTGCG ATTGTGTGAC TTGCTCCTCG GCGATGACTG GGAAATTCTC 
    TACTTCGAGC CGATGTGGAA AGTCATCCCG AGCAACAAGG CCATCCTGCC CATGATCTAC 
    CACAACCACC CCGAACACCC AGCCATCTTG AAGGCGGAGT ACGAGTTGAC GGACGAGCTG 
    CGCAAGCACG GGTACGCCAA GAAGCCGATT GTCGGCCGCG TGGGCAGCAA CGTTATCATC 
    ACCTCCGGCG ACGGCGTGGT GCACGCTGAG TCTGGTGGCA AATATGGCAA GCGCAACATG 
    ATTTACCAAC AGCTGTTTGA GCTGAAGAAG CAGGATGACT ACTACGCCAT CATCGGCGGC 
    TGGATGATCG GCGACGCCTT CAGCGGCACC GGCATCCGTG AGGACAAGTC AGTCATCACC 
    GGCGTGGACA GCCCCTTCGC TGCCGTTCGC ATCAAGACCG ACAAACTTCC GCACCCCGTG 
    ACGCTCAAGG ATATCGACAA GATGGCCGAG GACGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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