| Gene Symbol | POLR1B |
| Entrez Gene ID | 84172 |
| Full Name | RNA polymerase I subunit B |
| Synonyms | RPA135,RPA2,Rpo1-2 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]. |
| Disorder MIM: |
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POLR1B cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following POLR1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu07934 Clone ID Related Accession (Same CDS sequence) NM_001282776.2 , NM_001371971.1 , NM_001282776.1 Accession Version NM_001282776.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 5 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001282776.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.199570.1, AK301178.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269705.1 CDS 216..2990 Translation 
Target ORF information:
RefSeq Version NM_001282776.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 5, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_001282776.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu07934 Clone ID Related Accession (Same CDS sequence) NM_001282776.2 , NM_001371971.1 , NM_001282776.1 Accession Version NM_001371971.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 5 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.181044.1 [ECO:0000332] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001358900.1 CDS 531..3305 Translation
Target ORF information:
RefSeq Version NM_001371971.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 10, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001371971.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu07934 Clone ID Related Accession (Same CDS sequence) NM_001282776.2 , NM_001371971.1 , NM_001282776.1 Accession Version NM_001282776.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 5 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, AK301178.1, AC012442.7 and AA484631.1. On Sep 19, 2013 this sequence version replaced XM_005263817.1. Transcript Variant: This variant (5) lacks three exons in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (5) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.199570.1, AK301178.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269705.1 CDS 766..3540 Translation
Target ORF information:
RefSeq Version NM_001282776.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 5, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282776.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCACTGTG TGAGGGAAGA ACATTCCGCT GTCAATATGA ACCTCCACTA CTTGGAAAAT
GGCACTGTTA TGTTGAACTT TATTTACCGA AAAGAACTGT TCTTTCTTCC TTTGGGATTT
GCACTTAAGG CACTTGTCAG CTTTTCTGAT TATCAGATCT TTCAGGAGCT CATCAAAGGA
AAAGAGGATG ATTCTTTCCT TAGGAACTCT GTTTCTCAGA TGTTAAGGAT TGTAATGGAA
GAGGGTTGTT CGACACAAAA ACAGGTCCTT AACTACCTAG GTGAATGCTT CAGAGTAAAA
CTCAATGTTC CTGACTGGTA CCCAAATGAG CAAGCTGCGG AGTTCCTGTT TAACCAGTGC
ATCTGTATCC ACTTGAAATC CAATACTGAA AAGTTTTATA TGCTTTGTCT CATGACGCGA
AAGCTCTTTG CTTTAGCCAA AGGAGAGTGC ATGGAGGACA ATCCTGATAG TTTGGTGAAC
CAGGAAGTCC TCACACCGGG TCAGCTCTTC CTTATGTTCC TGAAGGAAAA ACTGGAAGGT
TGGTTAGTGT CTATTAAAAT AGCTTTTGAT AAGAAGGCTC AGAAGACCAG TGTTTCCATG
AACACTGACA ATTTGATGAG GATTTTTACA ATGGGCATAG ACCTTACAAA ACCATTTGAA
TACCTTTTTG CTACTGGGAA TCTGCGTTCT AAAACAGGTC TTGGCCTCCT ACAAGATTCT
GGACTTTGTG TTGTGGCTGA CAAGCTGAAC TTCATACGCT ACCTCTCCCA TTTCCGCTGC
GTGCACAGAG GGGCTGATTT TGCCAAGATG AGGACCACCA CAGTACGCAG GCTGCTGCCA
GAGTCCTGGG GCTTCCTTTG TCCCGTGCAT ACCCCAGACG GGGAGCCCTG TGGCCTGATG
AACCACCTAA CTGCCGTATG TGAGGTTGTC ACACAGTTTG TGTATACGGC ATCTATTCCA
GCTTTACTGT GCAACTTGGG GGTCACTCCC ATTGATGGAG CTCCCCACCG ATCATACAGT
GAGTGCTACC CTGTCCTGCT GGACGGTGTC ATGGTTGGCT GGGTGGATAA GGATCTTGCT
CCAGGCATCG CAGATTCTCT TCGTCATTTT AAGGTGTTGA GAGAGAAAAG AATTCCTCCC
TGGATGGAAG TGGTCCTTAT ACCCATGACA GGAAAACCAA GTCTGTACCC AGGATTGTTC
CTTTTTACCA CTCCTTGTAG ACTGGTACGG CCTGTGCAGA ACTTAGCATT GGGCAAAGAA
GAGCTAATTG GAACTATGGA ACAGATCTTC ATGAATGTCG CTATCTTTGA GGATGAAGTT
TTTGCTGGAG TTACCACACA CCAGGAACTC TTTCCACACA GCCTGCTGAG TGTGATTGCC
AACTTCATCC CTTTCTCTGA TCACAACCAG AGTCCACGGA ACATGTACCA ATGCCAGATG
GGTAAGCAAA CTATGGGCTT TCCACTTCTC ACTTATCAAG ACCGATCGGA TAACAAACTG
TATCGTCTTC AGACTCCTCA GAGTCCCTTG GTGAGACCCT CCATGTATGA TTATTATGAC
ATGGATAACT ATCCAATTGG GACCAATGCC ATCGTTGCTG TGATTTCTTA CACTGGCTAT
GATATGGAAG ATGCCATGAT TGTGAATAAG GCCTCTTGGG AACGAGGCTT TGCCCATGGA
AGTGTCTACA AGTCTGAGTT CATAGACCTC TCTGAAAAAA TTAAACAAGG AGATAGTAGC
CTGGTGTTTG GCATCAAACC TGGTGACCCA CGCGTTCTGC AGAAGTTAGA TGACGATGGA
TTGCCGTTTA TAGGAGCAAA ACTGCAGTAC GGAGATCCGT ATTACAGCTA CCTCAACCTC
AACACCGGGG AAAGTTTTGT GATGTACTAT AAGAGTAAAG AAAATTGTGT TGTGGATAAC
ATCAAAGTGT GCAGTAATGA CACTGGGAGT GGAAAATTCA AGTGTGTTTG CATCACTATG
AGAGTGCCTC GGAACCCAAC TATCGGAGAT AAATTTGCCA GTCGCCATGG GCAGAAGGGC
ATTTTAAGCA GATTGTGGCC GGCTGAGGAC ATGCCTTTTA CTGAGAGTGG GATGGTCCCA
GACATTCTGT TCAATCCCCA TGGTTTTCCA TCCCGCATGA CCATTGGGAT GTTAATTGAG
AGTATGGCCG GGAAGTCTGC AGCTTTGCAT GGTCTCTGCC ATGATGCTAC ACCCTTCATC
TTCTCAGAGG AGAACTCGGC CTTAGAATAC TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC
AATTTCTATG GCACCGAGAG GTTATATAGT GGCATCAGTG GGCTAGAACT GGAAGCAGAC
ATCTTCATAG GAGTGGTTTA TTATCAGCGC TTACGCCATA TGGTCTCAGA CAAATTTCAA
GTAAGGACAA CTGGAGCCCG AGACAGAGTC ACCAACCAGC CTATTGGGGG AAGAAATGTC
CAGGGTGGAA TCCGTTTTGG GGAGATGGAA CGGGATGCGC TTTTAGCTCA TGGTACATCT
TTTCTCCTTC ATGACCGCCT CTTCAACTGC TCAGATCGGT CGGTAGCCCA TGTGTGTGTG
AAGTGTGGCA GTTTACTCTC TCCACTGTTG GAGAAGCCAC CCCCTTCTTG GTCTGCCATG
CGCAACAGAA AATACAACTG TACTCTGTGT AGTCGCAGTG ACACTATCGA TACTGTTTCT
GTGCCTTATG TTTTTCGGTA TTTTGTAGCT GAACTGGCAG CTATGAACAT CAAAGTGAAA
CTGGATGTTG TTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08012 Clone ID Related Accession (Same CDS sequence) NM_001371970.1 , NM_001282777.1 , NM_001282777.2 , NM_001282779.1 , NM_001282779.2 Accession Version NM_001371970.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 6 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.93936.1 [ECO:0000332] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001358899.1 CDS 706..3696 Translation
Target ORF information:
RefSeq Version NM_001371970.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 9, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001371970.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08012 Clone ID Related Accession (Same CDS sequence) NM_001371970.1 , NM_001282777.1 , NM_001282777.2 , NM_001282779.1 , NM_001282779.2 Accession Version NM_001282777.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 6 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, BX647360.1, AC012442.7 and AA484631.1. On Sep 19, 2013 this sequence version replaced XM_005263814.1. Transcript Variant: This variant (6) includes an additional exon in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (6) is shorter at the N-terminus, compared to isoform 1. Both variants 6 and 7 encode isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.133382.1, BX647360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269706.1 CDS 1090..4080 Translation
Target ORF information:
RefSeq Version NM_001282777.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282777.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08012 Clone ID Related Accession (Same CDS sequence) NM_001371970.1 , NM_001282777.1 , NM_001282777.2 , NM_001282779.1 , NM_001282779.2 Accession Version NM_001282777.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 6 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001282777.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1640341.1, SRR1803616.133382.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269706.1 CDS 540..3530 Translation
Target ORF information:
RefSeq Version NM_001282777.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282777.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08012 Clone ID Related Accession (Same CDS sequence) NM_001371970.1 , NM_001282777.1 , NM_001282777.2 , NM_001282779.1 , NM_001282779.2 Accession Version NM_001282779.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 6 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, AC012442.7, AK294027.1 and AA484631.1. On Sep 19, 2013 this sequence version replaced XM_005263815.1. Transcript Variant: This variant (7) uses an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (6) is shorter at the N-terminus, compared to isoform 1. Both variants 6 and 7 encode isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647683.1, AK294027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269708.1 CDS 927..3917 Translation
Target ORF information:
RefSeq Version NM_001282779.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 7, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282779.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08012 Clone ID Related Accession (Same CDS sequence) NM_001371970.1 , NM_001282777.1 , NM_001282777.2 , NM_001282779.1 , NM_001282779.2 Accession Version NM_001282779.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 6 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001282779.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647683.1, AK294027.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269708.1 CDS 377..3367 Translation
Target ORF information:
RefSeq Version NM_001282779.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 7, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282779.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGGTGAAAT CCAAGCTTTG CAACTTACGT AACCTTCCCC CACAAGCCCT CATTGAGCAC
CATGAGGAGG CAGAGGAAAT GGGGGGCTAT TTTATAATCA ATGGCATTGA AAAAGTCATC
CGAATGTTGA TTATGCCTCG GAGAAATTTT CCCATTGCAA TGATAAGACC AAAATGGAAA
ACCAGAGGGC CTGGTTATAC TCAGTATGGA GTTTCAATGC ACTGTGTGAG GGAAGAACAT
TCCGCTGTCA ATATGAACCT CCACTACTTG GAAAATGGCA CTGTTATGTT GAACTTTATT
TACCGAAAAG AACTGTTCTT TCTTCCTTTG GGATTTGCAC TTAAGGCACT TGTCAGCTTT
TCTGATTATC AGATCTTTCA GGAGCTCATC AAAGGAAAAG AGGATGATTC TTTCCTTAGG
AACTCTGTTT CTCAGATGTT AAGGATTGTA ATGGAAGAGG GTTGTTCGAC ACAAAAACAG
GTCCTTAACT ACCTAGGTGA ATGCTTCAGA GTAAAACTCA ATGTTCCTGA CTGGTACCCA
AATGAGCAAG CTGCGGAGTT CCTGTTTAAC CAGTGCATCT GTATCCACTT GAAATCCAAT
ACTGAAAAGT TTTATATGCT TTGTCTCATG ACGCGAAAGC TCTTTGCTTT AGCCAAAGGA
GAGTGCATGG AGGACAATCC TGATAGTTTG GTGAACCAGG AAGTCCTCAC ACCGGGTCAG
CTCTTCCTTA TGTTCCTGAA GGAAAAACTG GAAGGTTGGT TAGTGTCTAT TAAAATAGCT
TTTGATAAGA AGGCTCAGAA GACCAGTGTT TCCATGAACA CTGACAATTT GATGAGGATT
TTTACAATGG GCATAGACCT TACAAAACCA TTTGAATACC TTTTTGCTAC TGGGAATCTG
CGTTCTAAAA CAGGTCTTGG CCTCCTACAA GATTCTGGAC TTTGTGTTGT GGCTGACAAG
CTGAACTTCA TACGCTACCT CTCCCATTTC CGCTGCGTGC ACAGAGGGGC TGATTTTGCC
AAGATGAGGA CCACCACAGT ACGCAGGCTG CTGCCAGAGT CCTGGGGCTT CCTTTGTCCC
GTGCATACCC CAGACGGGGA GCCCTGTGGC CTGATGAACC ACCTAACTGC CGTATGTGAG
GTTGTCACAC AGTTTGTGTA TACGGCATCT ATTCCAGCTT TACTGTGCAA CTTGGGGGTC
ACTCCCATTG ATGGAGCTCC CCACCGATCA TACAGTGAGT GCTACCCTGT CCTGCTGGAC
GGTGTCATGG TTGGCTGGGT GGATAAGGAT CTTGCTCCAG GCATCGCAGA TTCTCTTCGT
CATTTTAAGG TGTTGAGAGA GAAAAGAATT CCTCCCTGGA TGGAAGTGGT CCTTATACCC
ATGACAGGAA AACCAAGTCT GTACCCAGGA TTGTTCCTTT TTACCACTCC TTGTAGACTG
GTACGGCCTG TGCAGAACTT AGCATTGGGC AAAGAAGAGC TAATTGGAAC TATGGAACAG
ATCTTCATGA ATGTCGCTAT CTTTGAGGAT GAAGTTTTTG CTGGAGTTAC CACACACCAG
GAACTCTTTC CACACAGCCT GCTGAGTGTG ATTGCCAACT TCATCCCTTT CTCTGATCAC
AACCAGAGTC CACGGAACAT GTACCAATGC CAGATGGGTA AGCAAACTAT GGGCTTTCCA
CTTCTCACTT ATCAAGACCG ATCGGATAAC AAACTGTATC GTCTTCAGAC TCCTCAGAGT
CCCTTGGTGA GACCCTCCAT GTATGATTAT TATGACATGG ATAACTATCC AATTGGGACC
AATGCCATCG TTGCTGTGAT TTCTTACACT GGCTATGATA TGGAAGATGC CATGATTGTG
AATAAGGCCT CTTGGGAACG AGGCTTTGCC CATGGAAGTG TCTACAAGTC TGAGTTCATA
GACCTCTCTG AAAAAATTAA ACAAGGAGAT AGTAGCCTGG TGTTTGGCAT CAAACCTGGT
GACCCACGCG TTCTGCAGAA GTTAGATGAC GATGGATTGC CGTTTATAGG AGCAAAACTG
CAGTACGGAG ATCCGTATTA CAGCTACCTC AACCTCAACA CCGGGGAAAG TTTTGTGATG
TACTATAAGA GTAAAGAAAA TTGTGTTGTG GATAACATCA AAGTGTGCAG TAATGACACT
GGGAGTGGAA AATTCAAGTG TGTTTGCATC ACTATGAGAG TGCCTCGGAA CCCAACTATC
GGAGATAAAT TTGCCAGTCG CCATGGGCAG AAGGGCATTT TAAGCAGATT GTGGCCGGCT
GAGGACATGC CTTTTACTGA GAGTGGGATG GTCCCAGACA TTCTGTTCAA TCCCCATGGT
TTTCCATCCC GCATGACCAT TGGGATGTTA ATTGAGAGTA TGGCCGGGAA GTCTGCAGCT
TTGCATGGTC TCTGCCATGA TGCTACACCC TTCATCTTCT CAGAGGAGAA CTCGGCCTTA
GAATACTTTG GTGAGATGTT AAAGGCTGCT GGCTACAATT TCTATGGCAC CGAGAGGTTA
TATAGTGGCA TCAGTGGGCT AGAACTGGAA GCAGACATCT TCATAGGAGT GGTTTATTAT
CAGCGCTTAC GCCATATGGT CTCAGACAAA TTTCAAGTAA GGACAACTGG AGCCCGAGAC
AGAGTCACCA ACCAGCCTAT TGGGGGAAGA AATGTCCAGG GTGGAATCCG TTTTGGGGAG
ATGGAACGGG ATGCGCTTTT AGCTCATGGT ACATCTTTTC TCCTTCATGA CCGCCTCTTC
AACTGCTCAG ATCGGTCGGT AGCCCATGTG TGTGTGAAGT GTGGCAGTTT ACTCTCTCCA
CTGTTGGAGA AGCCACCCCC TTCTTGGTCT GCCATGCGCA ACAGAAAATA CAACTGTACT
CTGTGTAGTC GCAGTGACAC TATCGATACT GTTTCTGTGC CTTATGTTTT TCGGTATTTT
GTAGCTGAAC TGGCAGCTAT GAACATCAAA GTGAAACTGG ATGTTGTTTA AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08411 Clone ID Related Accession (Same CDS sequence) NM_001282774.1 , NM_001282774.2 Accession Version NM_001282774.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 4 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, BC110833.1, AC012442.7 and AA484631.1. On Sep 19, 2013 this sequence version replaced XM_005263816.1. Transcript Variant: This variant (4) lacks two alternate exons that result in a short frameshift in the central coding region, and also uses an alternate splice site that restores the reading frame in the remaining coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820701 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGG GTCACTCCCA TTGATGGAGC TCCCCACCGA TCATACAGTG
TTGAGAGAGA AAAGAATTCC TCCCTGGATG GAAGTGGTCC TTATACCCAT GACAGGAAAA
CCAAGTCTGT ACCCAGGATT GTTCCTTTTT ACCACTCCTT GTAGACTGGT ACGGCCTGTG
CAGAACTTAG CATTGGGCAA AGAAGAGCTA ATTGGAACTA TGGAACAGAT CTTCATGAAT
GTCGCTATCT TTGAGGATGA AGTTTTTGCT GGAGTTACCA CACACCAGGA ACTCTTTCCA
CACAGCCTGC TGAGTGTGAT TGCCAACTTC ATCCCTTTCT CTGATCACAA CCAGAGTCCA
CGGAACATGT ACCAATGCCA GATGGGTAAG CAAACTATGG GCTTTCCACT TCTCACTTAT
CAAGACCGAT CGGATAACAA ACTGTATCGT CTTCAGACTC CTCAGAGTCC CTTGGTGAGA
CCCTCCATGT ATGATTATTA TGACATGGAT AACTATCCAA TTGGGACCAA TGCCATCGTT
GCTGTGATTT CTTACACTGG CTATGATATG GAAGATGCCA TGATTGTGAA TAAGGCCTCT
TGGGAACGAG GCTTTGCCCA TGGAAGTGTC TACAAGTCTG AGTTCATAGA CCTCTCTGAA
AAAATTAAAC AAGGAGATAG TAGCCTGGTG TTTGGCATCA AACCTGGTGA CCCACGCGTT
CTGCAGAAGT TAGATGACGA TGGATTGCCG TTTATAGGAG CAAAACTGCA GTACGGAGAT
CCGTATTACA GCTACCTCAA CCTCAACACC GGGGAAAGTT TTGTGATGTA CTATAAGAGT
AAAGAAAATT GTGTTGTGGA TAACATCAAA GTGTGCAGTA ATGACACTGG GAGTGGAAAA
TTCAAGTGTG TTTGCATCAC TATGAGAGTG CCTCGGAACC CAACTATCGG AGATAAATTT
GCCAGTCGCC ATGGGCAGAA GGGCATTTTA AGCAGATTGT GGCCGGCTGA GGACATGCCT
TTTACTGAGA GTGGGATGGT CCCAGACATT CTGTTCAATC CCCATGGTTT TCCATCCCGC
ATGACCATTG GGATGTTAAT TGAGAGTATG GCCGGGAAGT CTGCAGCTTT GCATGGTCTC
TGCCATGATG CTACACCCTT CATCTTCTCA GAGGAGAACT CGGCCTTAGA ATACTTTGGT
GAGATGTTAA AGGCTGCTGG CTACAATTTC TATGGCACCG AGAGGTTATA TAGTGGCATC
AGTGGGCTAG AACTGGAAGC AGACATCTTC ATAGGAGTGG TTTATTATCA GCGCTTACGC
CATATGGTCT CAGACAAATT TCAAGTAAGG ACAACTGGAG CCCGAGACAG AGTCACCAAC
CAGCCTATTG GGGGAAGAAA TGTCCAGGGT GGAATCCGTT TTGGGGAGAT GGAACGGGAT
GCGCTTTTAG CTCATGGTAC ATCTTTTCTC CTTCATGACC GCCTCTTCAA CTGCTCAGAT
CGGTCGGTAG CCCATGTGTG TGTGAAGTGT GGCAGTTTAC TCTCTCCACT GTTGGAGAAG
CCACCCCCTT CTTGGTCTGC CATGCGCAAC AGAAAATACA ACTGTACTCT GTGTAGTCGC
AGTGACACTA TCGATACTGT TTCTGTGCCT TATGTTTTTC GGTATTTTGT AGCTGAACTG
GCAGCTATGA ACATCAAAGT GAAACTGGAT GTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269703.1 CDS 581..3439 Translation
Target ORF information:
RefSeq Version NM_001282774.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282774.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGG GTCACTCCCA TTGATGGAGC TCCCCACCGA TCATACAGTG
TTGAGAGAGA AAAGAATTCC TCCCTGGATG GAAGTGGTCC TTATACCCAT GACAGGAAAA
CCAAGTCTGT ACCCAGGATT GTTCCTTTTT ACCACTCCTT GTAGACTGGT ACGGCCTGTG
CAGAACTTAG CATTGGGCAA AGAAGAGCTA ATTGGAACTA TGGAACAGAT CTTCATGAAT
GTCGCTATCT TTGAGGATGA AGTTTTTGCT GGAGTTACCA CACACCAGGA ACTCTTTCCA
CACAGCCTGC TGAGTGTGAT TGCCAACTTC ATCCCTTTCT CTGATCACAA CCAGAGTCCA
CGGAACATGT ACCAATGCCA GATGGGTAAG CAAACTATGG GCTTTCCACT TCTCACTTAT
CAAGACCGAT CGGATAACAA ACTGTATCGT CTTCAGACTC CTCAGAGTCC CTTGGTGAGA
CCCTCCATGT ATGATTATTA TGACATGGAT AACTATCCAA TTGGGACCAA TGCCATCGTT
GCTGTGATTT CTTACACTGG CTATGATATG GAAGATGCCA TGATTGTGAA TAAGGCCTCT
TGGGAACGAG GCTTTGCCCA TGGAAGTGTC TACAAGTCTG AGTTCATAGA CCTCTCTGAA
AAAATTAAAC AAGGAGATAG TAGCCTGGTG TTTGGCATCA AACCTGGTGA CCCACGCGTT
CTGCAGAAGT TAGATGACGA TGGATTGCCG TTTATAGGAG CAAAACTGCA GTACGGAGAT
CCGTATTACA GCTACCTCAA CCTCAACACC GGGGAAAGTT TTGTGATGTA CTATAAGAGT
AAAGAAAATT GTGTTGTGGA TAACATCAAA GTGTGCAGTA ATGACACTGG GAGTGGAAAA
TTCAAGTGTG TTTGCATCAC TATGAGAGTG CCTCGGAACC CAACTATCGG AGATAAATTT
GCCAGTCGCC ATGGGCAGAA GGGCATTTTA AGCAGATTGT GGCCGGCTGA GGACATGCCT
TTTACTGAGA GTGGGATGGT CCCAGACATT CTGTTCAATC CCCATGGTTT TCCATCCCGC
ATGACCATTG GGATGTTAAT TGAGAGTATG GCCGGGAAGT CTGCAGCTTT GCATGGTCTC
TGCCATGATG CTACACCCTT CATCTTCTCA GAGGAGAACT CGGCCTTAGA ATACTTTGGT
GAGATGTTAA AGGCTGCTGG CTACAATTTC TATGGCACCG AGAGGTTATA TAGTGGCATC
AGTGGGCTAG AACTGGAAGC AGACATCTTC ATAGGAGTGG TTTATTATCA GCGCTTACGC
CATATGGTCT CAGACAAATT TCAAGTAAGG ACAACTGGAG CCCGAGACAG AGTCACCAAC
CAGCCTATTG GGGGAAGAAA TGTCCAGGGT GGAATCCGTT TTGGGGAGAT GGAACGGGAT
GCGCTTTTAG CTCATGGTAC ATCTTTTCTC CTTCATGACC GCCTCTTCAA CTGCTCAGAT
CGGTCGGTAG CCCATGTGTG TGTGAAGTGT GGCAGTTTAC TCTCTCCACT GTTGGAGAAG
CCACCCCCTT CTTGGTCTGC CATGCGCAAC AGAAAATACA ACTGTACTCT GTGTAGTCGC
AGTGACACTA TCGATACTGT TTCTGTGCCT TATGTTTTTC GGTATTTTGT AGCTGAACTG
GCAGCTATGA ACATCAAAGT GAAACTGGAT GTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08411 Clone ID Related Accession (Same CDS sequence) NM_001282774.1 , NM_001282774.2 Accession Version NM_001282774.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 4 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001282774.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110833.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGG GTCACTCCCA TTGATGGAGC TCCCCACCGA TCATACAGTG
TTGAGAGAGA AAAGAATTCC TCCCTGGATG GAAGTGGTCC TTATACCCAT GACAGGAAAA
CCAAGTCTGT ACCCAGGATT GTTCCTTTTT ACCACTCCTT GTAGACTGGT ACGGCCTGTG
CAGAACTTAG CATTGGGCAA AGAAGAGCTA ATTGGAACTA TGGAACAGAT CTTCATGAAT
GTCGCTATCT TTGAGGATGA AGTTTTTGCT GGAGTTACCA CACACCAGGA ACTCTTTCCA
CACAGCCTGC TGAGTGTGAT TGCCAACTTC ATCCCTTTCT CTGATCACAA CCAGAGTCCA
CGGAACATGT ACCAATGCCA GATGGGTAAG CAAACTATGG GCTTTCCACT TCTCACTTAT
CAAGACCGAT CGGATAACAA ACTGTATCGT CTTCAGACTC CTCAGAGTCC CTTGGTGAGA
CCCTCCATGT ATGATTATTA TGACATGGAT AACTATCCAA TTGGGACCAA TGCCATCGTT
GCTGTGATTT CTTACACTGG CTATGATATG GAAGATGCCA TGATTGTGAA TAAGGCCTCT
TGGGAACGAG GCTTTGCCCA TGGAAGTGTC TACAAGTCTG AGTTCATAGA CCTCTCTGAA
AAAATTAAAC AAGGAGATAG TAGCCTGGTG TTTGGCATCA AACCTGGTGA CCCACGCGTT
CTGCAGAAGT TAGATGACGA TGGATTGCCG TTTATAGGAG CAAAACTGCA GTACGGAGAT
CCGTATTACA GCTACCTCAA CCTCAACACC GGGGAAAGTT TTGTGATGTA CTATAAGAGT
AAAGAAAATT GTGTTGTGGA TAACATCAAA GTGTGCAGTA ATGACACTGG GAGTGGAAAA
TTCAAGTGTG TTTGCATCAC TATGAGAGTG CCTCGGAACC CAACTATCGG AGATAAATTT
GCCAGTCGCC ATGGGCAGAA GGGCATTTTA AGCAGATTGT GGCCGGCTGA GGACATGCCT
TTTACTGAGA GTGGGATGGT CCCAGACATT CTGTTCAATC CCCATGGTTT TCCATCCCGC
ATGACCATTG GGATGTTAAT TGAGAGTATG GCCGGGAAGT CTGCAGCTTT GCATGGTCTC
TGCCATGATG CTACACCCTT CATCTTCTCA GAGGAGAACT CGGCCTTAGA ATACTTTGGT
GAGATGTTAA AGGCTGCTGG CTACAATTTC TATGGCACCG AGAGGTTATA TAGTGGCATC
AGTGGGCTAG AACTGGAAGC AGACATCTTC ATAGGAGTGG TTTATTATCA GCGCTTACGC
CATATGGTCT CAGACAAATT TCAAGTAAGG ACAACTGGAG CCCGAGACAG AGTCACCAAC
CAGCCTATTG GGGGAAGAAA TGTCCAGGGT GGAATCCGTT TTGGGGAGAT GGAACGGGAT
GCGCTTTTAG CTCATGGTAC ATCTTTTCTC CTTCATGACC GCCTCTTCAA CTGCTCAGAT
CGGTCGGTAG CCCATGTGTG TGTGAAGTGT GGCAGTTTAC TCTCTCCACT GTTGGAGAAG
CCACCCCCTT CTTGGTCTGC CATGCGCAAC AGAAAATACA ACTGTACTCT GTGTAGTCGC
AGTGACACTA TCGATACTGT TTCTGTGCCT TATGTTTTTC GGTATTTTGT AGCTGAACTG
GCAGCTATGA ACATCAAAGT GAAACTGGAT GTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269703.1 CDS 31..2889 Translation
Target ORF information:
RefSeq Version NM_001282774.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282774.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGG GTCACTCCCA TTGATGGAGC TCCCCACCGA TCATACAGTG
TTGAGAGAGA AAAGAATTCC TCCCTGGATG GAAGTGGTCC TTATACCCAT GACAGGAAAA
CCAAGTCTGT ACCCAGGATT GTTCCTTTTT ACCACTCCTT GTAGACTGGT ACGGCCTGTG
CAGAACTTAG CATTGGGCAA AGAAGAGCTA ATTGGAACTA TGGAACAGAT CTTCATGAAT
GTCGCTATCT TTGAGGATGA AGTTTTTGCT GGAGTTACCA CACACCAGGA ACTCTTTCCA
CACAGCCTGC TGAGTGTGAT TGCCAACTTC ATCCCTTTCT CTGATCACAA CCAGAGTCCA
CGGAACATGT ACCAATGCCA GATGGGTAAG CAAACTATGG GCTTTCCACT TCTCACTTAT
CAAGACCGAT CGGATAACAA ACTGTATCGT CTTCAGACTC CTCAGAGTCC CTTGGTGAGA
CCCTCCATGT ATGATTATTA TGACATGGAT AACTATCCAA TTGGGACCAA TGCCATCGTT
GCTGTGATTT CTTACACTGG CTATGATATG GAAGATGCCA TGATTGTGAA TAAGGCCTCT
TGGGAACGAG GCTTTGCCCA TGGAAGTGTC TACAAGTCTG AGTTCATAGA CCTCTCTGAA
AAAATTAAAC AAGGAGATAG TAGCCTGGTG TTTGGCATCA AACCTGGTGA CCCACGCGTT
CTGCAGAAGT TAGATGACGA TGGATTGCCG TTTATAGGAG CAAAACTGCA GTACGGAGAT
CCGTATTACA GCTACCTCAA CCTCAACACC GGGGAAAGTT TTGTGATGTA CTATAAGAGT
AAAGAAAATT GTGTTGTGGA TAACATCAAA GTGTGCAGTA ATGACACTGG GAGTGGAAAA
TTCAAGTGTG TTTGCATCAC TATGAGAGTG CCTCGGAACC CAACTATCGG AGATAAATTT
GCCAGTCGCC ATGGGCAGAA GGGCATTTTA AGCAGATTGT GGCCGGCTGA GGACATGCCT
TTTACTGAGA GTGGGATGGT CCCAGACATT CTGTTCAATC CCCATGGTTT TCCATCCCGC
ATGACCATTG GGATGTTAAT TGAGAGTATG GCCGGGAAGT CTGCAGCTTT GCATGGTCTC
TGCCATGATG CTACACCCTT CATCTTCTCA GAGGAGAACT CGGCCTTAGA ATACTTTGGT
GAGATGTTAA AGGCTGCTGG CTACAATTTC TATGGCACCG AGAGGTTATA TAGTGGCATC
AGTGGGCTAG AACTGGAAGC AGACATCTTC ATAGGAGTGG TTTATTATCA GCGCTTACGC
CATATGGTCT CAGACAAATT TCAAGTAAGG ACAACTGGAG CCCGAGACAG AGTCACCAAC
CAGCCTATTG GGGGAAGAAA TGTCCAGGGT GGAATCCGTT TTGGGGAGAT GGAACGGGAT
GCGCTTTTAG CTCATGGTAC ATCTTTTCTC CTTCATGACC GCCTCTTCAA CTGCTCAGAT
CGGTCGGTAG CCCATGTGTG TGTGAAGTGT GGCAGTTTAC TCTCTCCACT GTTGGAGAAG
CCACCCCCTT CTTGGTCTGC CATGCGCAAC AGAAAATACA ACTGTACTCT GTGTAGTCGC
AGTGACACTA TCGATACTGT TTCTGTGCCT TATGTTTTTC GGTATTTTGT AGCTGAACTG
GCAGCTATGA ACATCAAAGT GAAACTGGAT GTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu07836 Clone ID Related Accession (Same CDS sequence) NM_001137604.3 , NM_001137604.2 Accession Version NM_001137604.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 2 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001137604.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022533.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
GATATCAACT GGGCAGTGAA TGGAATCTCA AAAGGAATCA TTAAGCAGTT TCTTGGCTAT
GTTCCCATCA TGGTGAAATC CAAGCTTTGC AACTTACGTA ACCTTCCCCC ACAAGCCCTC
ATTGAGCACC ATGAGGAGGC AGAGGAAATG GGGGGCTATT TTATAATCAA TGGCATTGAA
AAAGTCATCC GAATGTTGAT TATGCCTCGG AGAAATTTTC CCATTGCAAT GATAAGACCA
AAATGGAAAA CCAGAGGGCC TGGTTATACT CAGTATGGAG TTTCAATGCA CTGTGTGAGG
GAAGAACATT CCGCTGTCAA TATGAACCTC CACTACTTGG AAAATGGCAC TGTTATGTTG
AACTTTATTT ACCGAAAAGA ACTGTTCTTT CTTCCTTTGG GATTTGCACT TAAGGCACTT
GTCAGCTTTT CTGATTATCA GATCTTTCAG GAGCTCATCA AAGGAAAAGA GGATGATTCT
TTCCTTAGGA ACTCTGTTTC TCAGATGTTA AGGATTGTAA TGGAAGAGGG TTGTTCGACA
CAAAAACAGG TCCTTAACTA CCTAGGTGAA TGCTTCAGAG TAAAACTCAA TGTTCCTGAC
TGGTACCCAA ATGAGCAAGC TGCGGAGTTC CTGTTTAACC AGTGCATCTG TATCCACTTG
AAATCCAATA CTGAAAAGTT TTATATGCTT TGTCTCATGA CGCGAAAGCT CTTTGCTTTA
GCCAAAGGAG AGTGCATGGA GGACAATCCT GATAGTTTGG TGAACCAGGA AGTCCTCACA
CCGGGTCAGC TCTTCCTTAT GTTCCTGAAG GAAAAACTGG AAGGTTGGTT AGTGTCTATT
AAAATAGCTT TTGATAAGAA GGCTCAGAAG ACCAGTGTTT CCATGAACAC TGACAATTTG
ATGAGGATTT TTACAATGGG CATAGACCTT ACAAAACCAT TTGAATACCT TTTTGCTACT
GGGAATCTGC GTTCTAAAAC AGGTCTTGGC CTCCTACAAG ATTCTGGACT TTGTGTTGTG
GCTGACAAGC TGAACTTCAT ACGCTACCTC TCCCATTTCC GCTGCGTGCA CAGAGGGGCT
GATTTTGCCA AGATGAGGAC CACCACAGTA CGCAGGCTGC TGCCAGAGTC CTGGGGCTTC
CTTTGTCCCG TGCATACCCC AGACGGGGAG CCCTGTGGCC TGATGAACCA CCTAACTGCC
GTATGTGAGG TTGTCACACA GTTTGTGTAT ACGGCATCTA TTCCAGCTTT ACTGTGCAAC
TTGGGGGTCA CTCCCATTGA TGGAGCTCCC CACCGATCAT ACAGTGAGTG CTACCCTGTC
CTGCTGGACG GTGTCATGGT TGGCTGGGTG GATAAGGATC TTGCTCCAGG CATCGCAGAT
TCTCTTCGTC ATTTTAAGGT GTTGAGAGAG AAAAGAATTC CTCCCTGGAT GGAAGTGGTC
CTTATACCCA TGACAGGAAA ACCAAGTCTG TACCCAGGAT TGTTCCTTTT TACCACTCCT
TGTAGACTGG TACGGCCTGT GCAGAACTTA GCATTGGGCA AAGAAGAGCT AATTGGAACT
ATGGAACAGA TCTTCATGAA TGTCGCTATC TTTGAGGATG AAGTTTTTGC TGGAGTTACC
ACACACCAGG AACTCTTTCC ACACAGCCTG CTGAGTGTGA TTGCCAACTT CATCCCTTTC
TCTGATCACA ACCAGAGTCC ACGGAACATG TACCAATGCC AGATGGGTAA GCAAACTATG
GGCTTTCCAC TTCTCACTTA TCAAGACCGA TCGGATAACA AACTGTATCG TCTTCAGACT
CCTCAGAGTC CCTTGGTGAG ACCCTCCATG TATGATTATT ATGACATGGA TAACTATCCA
ATTGGGACCA ATGCCATCGT TGCTGTGATT TCTTACACTG GCTATGATAT GGAAGATGCC
ATGATTGTGA ATAAGGCCTC TTGGGAACGA GGCTTTGCCC ATGGAAGTGT CTACAAGTCT
GAGTTCATAG ACCTCTCTGA AAAAATTAAA CAAGGAGATA GTAGCCTGGT GTTTGGCATC
AAACCTGGTG ACCCACGCGT TCTGCAGAAG TTAGATGACG ATGGATTGCC GTTTATAGGA
GCAAAACTGC AGTACGGAGA TCCGTATTAC AGCTACCTCA ACCTCAACAC CGGGGAAAGT
TTTGTGATGT ACTATAAGAG TAAAGAAAAT TGTGTTGTGG ATAACATCAA AGTGTGCAGT
AATGACACTG GGAGTGGAAA ATTCAAGTGT GTTTGCATCA CTATGAGAGT GCCTCGGAAC
CCAACTATCG GAGATAAATT TGCCAGTCGC CATGGGCAGA AGGGCATTTT AAGCAGATTG
TGGCCGGCTG AGGACATGCC TTTTACTGAG AGTGGGATGG TCCCAGACAT TCTGTTCAAT
CCCCATGGTT TTCCATCCCG CATGACCATT GGGATGTTAA TTGAGAGTAT GGCCGGGAAG
TCTGCAGCTT TGCATGGTCT CTGCCATGAT GCTACACCCT TCATCTTCTC AGAGGAGAAC
TCGGCCTTAG AATACTTTGG TGAGATGTTA AAGGCTGCTG GCTACAATTT CTATGGCACC
GAGAGGTTAT ATAGTGGCAT CAGTGGGCTA GAACTGGAAG CAGACATCTT CATAGGAGTG
GTTTATTATC AGCGCTTACG CCATATGGTC TCAGACAAAT TTCAAGTAAG GACAACTGGA
GCCCGAGACA GAGTCACCAA CCAGCCTATT GGGGGAAGAA ATGTCCAGGG TGGAATCCGT
TTTGGGGAGA TGGAACGGGA TGCGCTTTTA GCTCATGGTA CATCTTTTCT CCTTCATGAC
CGCCTCTTCA ACTGCTCAGA TCGGTCGGTA GCCCATGTGT GTGTGAAGTG TGGCAGTTTA
CTCTCTCCAC TGTTGGAGAA GCCACCCCCT TCTTGGTCTG CCATGCGCAA CAGAAAATAC
AACTGTACTC TGTGTAGTCG CAGTGACACT ATCGATACTG TTTCTGTGCC TTATGTTTTT
CGGTATTTTG TAGCTGAACT GGCAGCTATG AACATCAAAG TGAAACTGGA TGTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001131076.1 CDS 31..3270 Translation
Target ORF information:
RefSeq Version NM_001137604.3 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001137604.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
GATATCAACT GGGCAGTGAA TGGAATCTCA AAAGGAATCA TTAAGCAGTT TCTTGGCTAT
GTTCCCATCA TGGTGAAATC CAAGCTTTGC AACTTACGTA ACCTTCCCCC ACAAGCCCTC
ATTGAGCACC ATGAGGAGGC AGAGGAAATG GGGGGCTATT TTATAATCAA TGGCATTGAA
AAAGTCATCC GAATGTTGAT TATGCCTCGG AGAAATTTTC CCATTGCAAT GATAAGACCA
AAATGGAAAA CCAGAGGGCC TGGTTATACT CAGTATGGAG TTTCAATGCA CTGTGTGAGG
GAAGAACATT CCGCTGTCAA TATGAACCTC CACTACTTGG AAAATGGCAC TGTTATGTTG
AACTTTATTT ACCGAAAAGA ACTGTTCTTT CTTCCTTTGG GATTTGCACT TAAGGCACTT
GTCAGCTTTT CTGATTATCA GATCTTTCAG GAGCTCATCA AAGGAAAAGA GGATGATTCT
TTCCTTAGGA ACTCTGTTTC TCAGATGTTA AGGATTGTAA TGGAAGAGGG TTGTTCGACA
CAAAAACAGG TCCTTAACTA CCTAGGTGAA TGCTTCAGAG TAAAACTCAA TGTTCCTGAC
TGGTACCCAA ATGAGCAAGC TGCGGAGTTC CTGTTTAACC AGTGCATCTG TATCCACTTG
AAATCCAATA CTGAAAAGTT TTATATGCTT TGTCTCATGA CGCGAAAGCT CTTTGCTTTA
GCCAAAGGAG AGTGCATGGA GGACAATCCT GATAGTTTGG TGAACCAGGA AGTCCTCACA
CCGGGTCAGC TCTTCCTTAT GTTCCTGAAG GAAAAACTGG AAGGTTGGTT AGTGTCTATT
AAAATAGCTT TTGATAAGAA GGCTCAGAAG ACCAGTGTTT CCATGAACAC TGACAATTTG
ATGAGGATTT TTACAATGGG CATAGACCTT ACAAAACCAT TTGAATACCT TTTTGCTACT
GGGAATCTGC GTTCTAAAAC AGGTCTTGGC CTCCTACAAG ATTCTGGACT TTGTGTTGTG
GCTGACAAGC TGAACTTCAT ACGCTACCTC TCCCATTTCC GCTGCGTGCA CAGAGGGGCT
GATTTTGCCA AGATGAGGAC CACCACAGTA CGCAGGCTGC TGCCAGAGTC CTGGGGCTTC
CTTTGTCCCG TGCATACCCC AGACGGGGAG CCCTGTGGCC TGATGAACCA CCTAACTGCC
GTATGTGAGG TTGTCACACA GTTTGTGTAT ACGGCATCTA TTCCAGCTTT ACTGTGCAAC
TTGGGGGTCA CTCCCATTGA TGGAGCTCCC CACCGATCAT ACAGTGAGTG CTACCCTGTC
CTGCTGGACG GTGTCATGGT TGGCTGGGTG GATAAGGATC TTGCTCCAGG CATCGCAGAT
TCTCTTCGTC ATTTTAAGGT GTTGAGAGAG AAAAGAATTC CTCCCTGGAT GGAAGTGGTC
CTTATACCCA TGACAGGAAA ACCAAGTCTG TACCCAGGAT TGTTCCTTTT TACCACTCCT
TGTAGACTGG TACGGCCTGT GCAGAACTTA GCATTGGGCA AAGAAGAGCT AATTGGAACT
ATGGAACAGA TCTTCATGAA TGTCGCTATC TTTGAGGATG AAGTTTTTGC TGGAGTTACC
ACACACCAGG AACTCTTTCC ACACAGCCTG CTGAGTGTGA TTGCCAACTT CATCCCTTTC
TCTGATCACA ACCAGAGTCC ACGGAACATG TACCAATGCC AGATGGGTAA GCAAACTATG
GGCTTTCCAC TTCTCACTTA TCAAGACCGA TCGGATAACA AACTGTATCG TCTTCAGACT
CCTCAGAGTC CCTTGGTGAG ACCCTCCATG TATGATTATT ATGACATGGA TAACTATCCA
ATTGGGACCA ATGCCATCGT TGCTGTGATT TCTTACACTG GCTATGATAT GGAAGATGCC
ATGATTGTGA ATAAGGCCTC TTGGGAACGA GGCTTTGCCC ATGGAAGTGT CTACAAGTCT
GAGTTCATAG ACCTCTCTGA AAAAATTAAA CAAGGAGATA GTAGCCTGGT GTTTGGCATC
AAACCTGGTG ACCCACGCGT TCTGCAGAAG TTAGATGACG ATGGATTGCC GTTTATAGGA
GCAAAACTGC AGTACGGAGA TCCGTATTAC AGCTACCTCA ACCTCAACAC CGGGGAAAGT
TTTGTGATGT ACTATAAGAG TAAAGAAAAT TGTGTTGTGG ATAACATCAA AGTGTGCAGT
AATGACACTG GGAGTGGAAA ATTCAAGTGT GTTTGCATCA CTATGAGAGT GCCTCGGAAC
CCAACTATCG GAGATAAATT TGCCAGTCGC CATGGGCAGA AGGGCATTTT AAGCAGATTG
TGGCCGGCTG AGGACATGCC TTTTACTGAG AGTGGGATGG TCCCAGACAT TCTGTTCAAT
CCCCATGGTT TTCCATCCCG CATGACCATT GGGATGTTAA TTGAGAGTAT GGCCGGGAAG
TCTGCAGCTT TGCATGGTCT CTGCCATGAT GCTACACCCT TCATCTTCTC AGAGGAGAAC
TCGGCCTTAG AATACTTTGG TGAGATGTTA AAGGCTGCTG GCTACAATTT CTATGGCACC
GAGAGGTTAT ATAGTGGCAT CAGTGGGCTA GAACTGGAAG CAGACATCTT CATAGGAGTG
GTTTATTATC AGCGCTTACG CCATATGGTC TCAGACAAAT TTCAAGTAAG GACAACTGGA
GCCCGAGACA GAGTCACCAA CCAGCCTATT GGGGGAAGAA ATGTCCAGGG TGGAATCCGT
TTTGGGGAGA TGGAACGGGA TGCGCTTTTA GCTCATGGTA CATCTTTTCT CCTTCATGAC
CGCCTCTTCA ACTGCTCAGA TCGGTCGGTA GCCCATGTGT GTGTGAAGTG TGGCAGTTTA
CTCTCTCCAC TGTTGGAGAA GCCACCCCCT TCTTGGTCTG CCATGCGCAA CAGAAAATAC
AACTGTACTC TGTGTAGTCG CAGTGACACT ATCGATACTG TTTCTGTGCC TTATGTTTTT
CGGTATTTTG TAGCTGAACT GGCAGCTATG AACATCAAAG TGAAACTGGA TGTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu07836 Clone ID Related Accession (Same CDS sequence) NM_001137604.3 , NM_001137604.2 Accession Version NM_001137604.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 2 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, AC012442.7, AK022533.1 and AA484631.1. On Sep 19, 2013 this sequence version replaced NM_001137604.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
GATATCAACT GGGCAGTGAA TGGAATCTCA AAAGGAATCA TTAAGCAGTT TCTTGGCTAT
GTTCCCATCA TGGTGAAATC CAAGCTTTGC AACTTACGTA ACCTTCCCCC ACAAGCCCTC
ATTGAGCACC ATGAGGAGGC AGAGGAAATG GGGGGCTATT TTATAATCAA TGGCATTGAA
AAAGTCATCC GAATGTTGAT TATGCCTCGG AGAAATTTTC CCATTGCAAT GATAAGACCA
AAATGGAAAA CCAGAGGGCC TGGTTATACT CAGTATGGAG TTTCAATGCA CTGTGTGAGG
GAAGAACATT CCGCTGTCAA TATGAACCTC CACTACTTGG AAAATGGCAC TGTTATGTTG
AACTTTATTT ACCGAAAAGA ACTGTTCTTT CTTCCTTTGG GATTTGCACT TAAGGCACTT
GTCAGCTTTT CTGATTATCA GATCTTTCAG GAGCTCATCA AAGGAAAAGA GGATGATTCT
TTCCTTAGGA ACTCTGTTTC TCAGATGTTA AGGATTGTAA TGGAAGAGGG TTGTTCGACA
CAAAAACAGG TCCTTAACTA CCTAGGTGAA TGCTTCAGAG TAAAACTCAA TGTTCCTGAC
TGGTACCCAA ATGAGCAAGC TGCGGAGTTC CTGTTTAACC AGTGCATCTG TATCCACTTG
AAATCCAATA CTGAAAAGTT TTATATGCTT TGTCTCATGA CGCGAAAGCT CTTTGCTTTA
GCCAAAGGAG AGTGCATGGA GGACAATCCT GATAGTTTGG TGAACCAGGA AGTCCTCACA
CCGGGTCAGC TCTTCCTTAT GTTCCTGAAG GAAAAACTGG AAGGTTGGTT AGTGTCTATT
AAAATAGCTT TTGATAAGAA GGCTCAGAAG ACCAGTGTTT CCATGAACAC TGACAATTTG
ATGAGGATTT TTACAATGGG CATAGACCTT ACAAAACCAT TTGAATACCT TTTTGCTACT
GGGAATCTGC GTTCTAAAAC AGGTCTTGGC CTCCTACAAG ATTCTGGACT TTGTGTTGTG
GCTGACAAGC TGAACTTCAT ACGCTACCTC TCCCATTTCC GCTGCGTGCA CAGAGGGGCT
GATTTTGCCA AGATGAGGAC CACCACAGTA CGCAGGCTGC TGCCAGAGTC CTGGGGCTTC
CTTTGTCCCG TGCATACCCC AGACGGGGAG CCCTGTGGCC TGATGAACCA CCTAACTGCC
GTATGTGAGG TTGTCACACA GTTTGTGTAT ACGGCATCTA TTCCAGCTTT ACTGTGCAAC
TTGGGGGTCA CTCCCATTGA TGGAGCTCCC CACCGATCAT ACAGTGAGTG CTACCCTGTC
CTGCTGGACG GTGTCATGGT TGGCTGGGTG GATAAGGATC TTGCTCCAGG CATCGCAGAT
TCTCTTCGTC ATTTTAAGGT GTTGAGAGAG AAAAGAATTC CTCCCTGGAT GGAAGTGGTC
CTTATACCCA TGACAGGAAA ACCAAGTCTG TACCCAGGAT TGTTCCTTTT TACCACTCCT
TGTAGACTGG TACGGCCTGT GCAGAACTTA GCATTGGGCA AAGAAGAGCT AATTGGAACT
ATGGAACAGA TCTTCATGAA TGTCGCTATC TTTGAGGATG AAGTTTTTGC TGGAGTTACC
ACACACCAGG AACTCTTTCC ACACAGCCTG CTGAGTGTGA TTGCCAACTT CATCCCTTTC
TCTGATCACA ACCAGAGTCC ACGGAACATG TACCAATGCC AGATGGGTAA GCAAACTATG
GGCTTTCCAC TTCTCACTTA TCAAGACCGA TCGGATAACA AACTGTATCG TCTTCAGACT
CCTCAGAGTC CCTTGGTGAG ACCCTCCATG TATGATTATT ATGACATGGA TAACTATCCA
ATTGGGACCA ATGCCATCGT TGCTGTGATT TCTTACACTG GCTATGATAT GGAAGATGCC
ATGATTGTGA ATAAGGCCTC TTGGGAACGA GGCTTTGCCC ATGGAAGTGT CTACAAGTCT
GAGTTCATAG ACCTCTCTGA AAAAATTAAA CAAGGAGATA GTAGCCTGGT GTTTGGCATC
AAACCTGGTG ACCCACGCGT TCTGCAGAAG TTAGATGACG ATGGATTGCC GTTTATAGGA
GCAAAACTGC AGTACGGAGA TCCGTATTAC AGCTACCTCA ACCTCAACAC CGGGGAAAGT
TTTGTGATGT ACTATAAGAG TAAAGAAAAT TGTGTTGTGG ATAACATCAA AGTGTGCAGT
AATGACACTG GGAGTGGAAA ATTCAAGTGT GTTTGCATCA CTATGAGAGT GCCTCGGAAC
CCAACTATCG GAGATAAATT TGCCAGTCGC CATGGGCAGA AGGGCATTTT AAGCAGATTG
TGGCCGGCTG AGGACATGCC TTTTACTGAG AGTGGGATGG TCCCAGACAT TCTGTTCAAT
CCCCATGGTT TTCCATCCCG CATGACCATT GGGATGTTAA TTGAGAGTAT GGCCGGGAAG
TCTGCAGCTT TGCATGGTCT CTGCCATGAT GCTACACCCT TCATCTTCTC AGAGGAGAAC
TCGGCCTTAG AATACTTTGG TGAGATGTTA AAGGCTGCTG GCTACAATTT CTATGGCACC
GAGAGGTTAT ATAGTGGCAT CAGTGGGCTA GAACTGGAAG CAGACATCTT CATAGGAGTG
GTTTATTATC AGCGCTTACG CCATATGGTC TCAGACAAAT TTCAAGTAAG GACAACTGGA
GCCCGAGACA GAGTCACCAA CCAGCCTATT GGGGGAAGAA ATGTCCAGGG TGGAATCCGT
TTTGGGGAGA TGGAACGGGA TGCGCTTTTA GCTCATGGTA CATCTTTTCT CCTTCATGAC
CGCCTCTTCA ACTGCTCAGA TCGGTCGGTA GCCCATGTGT GTGTGAAGTG TGGCAGTTTA
CTCTCTCCAC TGTTGGAGAA GCCACCCCCT TCTTGGTCTG CCATGCGCAA CAGAAAATAC
AACTGTACTC TGTGTAGTCG CAGTGACACT ATCGATACTG TTTCTGTGCC TTATGTTTTT
CGGTATTTTG TAGCTGAACT GGCAGCTATG AACATCAAAG TGAAACTGGA TGTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001131076.1 CDS 581..3820 Translation
Target ORF information:
RefSeq Version NM_001137604.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001137604.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
GATATCAACT GGGCAGTGAA TGGAATCTCA AAAGGAATCA TTAAGCAGTT TCTTGGCTAT
GTTCCCATCA TGGTGAAATC CAAGCTTTGC AACTTACGTA ACCTTCCCCC ACAAGCCCTC
ATTGAGCACC ATGAGGAGGC AGAGGAAATG GGGGGCTATT TTATAATCAA TGGCATTGAA
AAAGTCATCC GAATGTTGAT TATGCCTCGG AGAAATTTTC CCATTGCAAT GATAAGACCA
AAATGGAAAA CCAGAGGGCC TGGTTATACT CAGTATGGAG TTTCAATGCA CTGTGTGAGG
GAAGAACATT CCGCTGTCAA TATGAACCTC CACTACTTGG AAAATGGCAC TGTTATGTTG
AACTTTATTT ACCGAAAAGA ACTGTTCTTT CTTCCTTTGG GATTTGCACT TAAGGCACTT
GTCAGCTTTT CTGATTATCA GATCTTTCAG GAGCTCATCA AAGGAAAAGA GGATGATTCT
TTCCTTAGGA ACTCTGTTTC TCAGATGTTA AGGATTGTAA TGGAAGAGGG TTGTTCGACA
CAAAAACAGG TCCTTAACTA CCTAGGTGAA TGCTTCAGAG TAAAACTCAA TGTTCCTGAC
TGGTACCCAA ATGAGCAAGC TGCGGAGTTC CTGTTTAACC AGTGCATCTG TATCCACTTG
AAATCCAATA CTGAAAAGTT TTATATGCTT TGTCTCATGA CGCGAAAGCT CTTTGCTTTA
GCCAAAGGAG AGTGCATGGA GGACAATCCT GATAGTTTGG TGAACCAGGA AGTCCTCACA
CCGGGTCAGC TCTTCCTTAT GTTCCTGAAG GAAAAACTGG AAGGTTGGTT AGTGTCTATT
AAAATAGCTT TTGATAAGAA GGCTCAGAAG ACCAGTGTTT CCATGAACAC TGACAATTTG
ATGAGGATTT TTACAATGGG CATAGACCTT ACAAAACCAT TTGAATACCT TTTTGCTACT
GGGAATCTGC GTTCTAAAAC AGGTCTTGGC CTCCTACAAG ATTCTGGACT TTGTGTTGTG
GCTGACAAGC TGAACTTCAT ACGCTACCTC TCCCATTTCC GCTGCGTGCA CAGAGGGGCT
GATTTTGCCA AGATGAGGAC CACCACAGTA CGCAGGCTGC TGCCAGAGTC CTGGGGCTTC
CTTTGTCCCG TGCATACCCC AGACGGGGAG CCCTGTGGCC TGATGAACCA CCTAACTGCC
GTATGTGAGG TTGTCACACA GTTTGTGTAT ACGGCATCTA TTCCAGCTTT ACTGTGCAAC
TTGGGGGTCA CTCCCATTGA TGGAGCTCCC CACCGATCAT ACAGTGAGTG CTACCCTGTC
CTGCTGGACG GTGTCATGGT TGGCTGGGTG GATAAGGATC TTGCTCCAGG CATCGCAGAT
TCTCTTCGTC ATTTTAAGGT GTTGAGAGAG AAAAGAATTC CTCCCTGGAT GGAAGTGGTC
CTTATACCCA TGACAGGAAA ACCAAGTCTG TACCCAGGAT TGTTCCTTTT TACCACTCCT
TGTAGACTGG TACGGCCTGT GCAGAACTTA GCATTGGGCA AAGAAGAGCT AATTGGAACT
ATGGAACAGA TCTTCATGAA TGTCGCTATC TTTGAGGATG AAGTTTTTGC TGGAGTTACC
ACACACCAGG AACTCTTTCC ACACAGCCTG CTGAGTGTGA TTGCCAACTT CATCCCTTTC
TCTGATCACA ACCAGAGTCC ACGGAACATG TACCAATGCC AGATGGGTAA GCAAACTATG
GGCTTTCCAC TTCTCACTTA TCAAGACCGA TCGGATAACA AACTGTATCG TCTTCAGACT
CCTCAGAGTC CCTTGGTGAG ACCCTCCATG TATGATTATT ATGACATGGA TAACTATCCA
ATTGGGACCA ATGCCATCGT TGCTGTGATT TCTTACACTG GCTATGATAT GGAAGATGCC
ATGATTGTGA ATAAGGCCTC TTGGGAACGA GGCTTTGCCC ATGGAAGTGT CTACAAGTCT
GAGTTCATAG ACCTCTCTGA AAAAATTAAA CAAGGAGATA GTAGCCTGGT GTTTGGCATC
AAACCTGGTG ACCCACGCGT TCTGCAGAAG TTAGATGACG ATGGATTGCC GTTTATAGGA
GCAAAACTGC AGTACGGAGA TCCGTATTAC AGCTACCTCA ACCTCAACAC CGGGGAAAGT
TTTGTGATGT ACTATAAGAG TAAAGAAAAT TGTGTTGTGG ATAACATCAA AGTGTGCAGT
AATGACACTG GGAGTGGAAA ATTCAAGTGT GTTTGCATCA CTATGAGAGT GCCTCGGAAC
CCAACTATCG GAGATAAATT TGCCAGTCGC CATGGGCAGA AGGGCATTTT AAGCAGATTG
TGGCCGGCTG AGGACATGCC TTTTACTGAG AGTGGGATGG TCCCAGACAT TCTGTTCAAT
CCCCATGGTT TTCCATCCCG CATGACCATT GGGATGTTAA TTGAGAGTAT GGCCGGGAAG
TCTGCAGCTT TGCATGGTCT CTGCCATGAT GCTACACCCT TCATCTTCTC AGAGGAGAAC
TCGGCCTTAG AATACTTTGG TGAGATGTTA AAGGCTGCTG GCTACAATTT CTATGGCACC
GAGAGGTTAT ATAGTGGCAT CAGTGGGCTA GAACTGGAAG CAGACATCTT CATAGGAGTG
GTTTATTATC AGCGCTTACG CCATATGGTC TCAGACAAAT TTCAAGTAAG GACAACTGGA
GCCCGAGACA GAGTCACCAA CCAGCCTATT GGGGGAAGAA ATGTCCAGGG TGGAATCCGT
TTTGGGGAGA TGGAACGGGA TGCGCTTTTA GCTCATGGTA CATCTTTTCT CCTTCATGAC
CGCCTCTTCA ACTGCTCAGA TCGGTCGGTA GCCCATGTGT GTGTGAAGTG TGGCAGTTTA
CTCTCTCCAC TGTTGGAGAA GCCACCCCCT TCTTGGTCTG CCATGCGCAA CAGAAAATAC
AACTGTACTC TGTGTAGTCG CAGTGACACT ATCGATACTG TTTCTGTGCC TTATGTTTTT
CGGTATTTTG TAGCTGAACT GGCAGCTATG AACATCAAAG TGAAACTGGA TGTTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08732 Clone ID Related Accession (Same CDS sequence) NM_019014.5 , NM_019014.6 Accession Version NM_019014.5 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1556553600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 1 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK128044.1, BC110833.1, AK294027.1, AC012442.7 and AA484631.1. On Sep 19, 2013 this sequence version replaced NM_019014.4. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128044.1, SRR1660807.237976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGGTGT TGAGAGAGAA AAGAATTCCT CCCTGGATGG AAGTGGTCCT TATACCCATG
ACAGGAAAAC CAAGTCTGTA CCCAGGATTG TTCCTTTTTA CCACTCCTTG TAGACTGGTA
CGGCCTGTGC AGAACTTAGC ATTGGGCAAA GAAGAGCTAA TTGGAACTAT GGAACAGATC
TTCATGAATG TCGCTATCTT TGAGGATGAA GTTTTTGCTG GAGTTACCAC ACACCAGGAA
CTCTTTCCAC ACAGCCTGCT GAGTGTGATT GCCAACTTCA TCCCTTTCTC TGATCACAAC
CAGAGTCCAC GGAACATGTA CCAATGCCAG ATGGGTAAGC AAACTATGGG CTTTCCACTT
CTCACTTATC AAGACCGATC GGATAACAAA CTGTATCGTC TTCAGACTCC TCAGAGTCCC
TTGGTGAGAC CCTCCATGTA TGATTATTAT GACATGGATA ACTATCCAAT TGGGACCAAT
GCCATCGTTG CTGTGATTTC TTACACTGGC TATGATATGG AAGATGCCAT GATTGTGAAT
AAGGCCTCTT GGGAACGAGG CTTTGCCCAT GGAAGTGTCT ACAAGTCTGA GTTCATAGAC
CTCTCTGAAA AAATTAAACA AGGAGATAGT AGCCTGGTGT TTGGCATCAA ACCTGGTGAC
CCACGCGTTC TGCAGAAGTT AGATGACGAT GGATTGCCGT TTATAGGAGC AAAACTGCAG
TACGGAGATC CGTATTACAG CTACCTCAAC CTCAACACCG GGGAAAGTTT TGTGATGTAC
TATAAGAGTA AAGAAAATTG TGTTGTGGAT AACATCAAAG TGTGCAGTAA TGACACTGGG
AGTGGAAAAT TCAAGTGTGT TTGCATCACT ATGAGAGTGC CTCGGAACCC AACTATCGGA
GATAAATTTG CCAGTCGCCA TGGGCAGAAG GGCATTTTAA GCAGATTGTG GCCGGCTGAG
GACATGCCTT TTACTGAGAG TGGGATGGTC CCAGACATTC TGTTCAATCC CCATGGTTTT
CCATCCCGCA TGACCATTGG GATGTTAATT GAGAGTATGG CCGGGAAGTC TGCAGCTTTG
CATGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCAG AGGAGAACTC GGCCTTAGAA
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAATTTCT ATGGCACCGA GAGGTTATAT
AGTGGCATCA GTGGGCTAGA ACTGGAAGCA GACATCTTCA TAGGAGTGGT TTATTATCAG
CGCTTACGCC ATATGGTCTC AGACAAATTT CAAGTAAGGA CAACTGGAGC CCGAGACAGA
GTCACCAACC AGCCTATTGG GGGAAGAAAT GTCCAGGGTG GAATCCGTTT TGGGGAGATG
GAACGGGATG CGCTTTTAGC TCATGGTACA TCTTTTCTCC TTCATGACCG CCTCTTCAAC
TGCTCAGATC GGTCGGTAGC CCATGTGTGT GTGAAGTGTG GCAGTTTACT CTCTCCACTG
TTGGAGAAGC CACCCCCTTC TTGGTCTGCC ATGCGCAACA GAAAATACAA CTGTACTCTG
TGTAGTCGCA GTGACACTAT CGATACTGTT TCTGTGCCTT ATGTTTTTCG GTATTTTGTA
GCTGAACTGG CAGCTATGAA CATCAAAGTG AAACTGGATG TTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_061887.2 CDS 581..3988 Translation
Target ORF information:
RefSeq Version NM_019014.5 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_019014.5
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGGTGT TGAGAGAGAA AAGAATTCCT CCCTGGATGG AAGTGGTCCT TATACCCATG
ACAGGAAAAC CAAGTCTGTA CCCAGGATTG TTCCTTTTTA CCACTCCTTG TAGACTGGTA
CGGCCTGTGC AGAACTTAGC ATTGGGCAAA GAAGAGCTAA TTGGAACTAT GGAACAGATC
TTCATGAATG TCGCTATCTT TGAGGATGAA GTTTTTGCTG GAGTTACCAC ACACCAGGAA
CTCTTTCCAC ACAGCCTGCT GAGTGTGATT GCCAACTTCA TCCCTTTCTC TGATCACAAC
CAGAGTCCAC GGAACATGTA CCAATGCCAG ATGGGTAAGC AAACTATGGG CTTTCCACTT
CTCACTTATC AAGACCGATC GGATAACAAA CTGTATCGTC TTCAGACTCC TCAGAGTCCC
TTGGTGAGAC CCTCCATGTA TGATTATTAT GACATGGATA ACTATCCAAT TGGGACCAAT
GCCATCGTTG CTGTGATTTC TTACACTGGC TATGATATGG AAGATGCCAT GATTGTGAAT
AAGGCCTCTT GGGAACGAGG CTTTGCCCAT GGAAGTGTCT ACAAGTCTGA GTTCATAGAC
CTCTCTGAAA AAATTAAACA AGGAGATAGT AGCCTGGTGT TTGGCATCAA ACCTGGTGAC
CCACGCGTTC TGCAGAAGTT AGATGACGAT GGATTGCCGT TTATAGGAGC AAAACTGCAG
TACGGAGATC CGTATTACAG CTACCTCAAC CTCAACACCG GGGAAAGTTT TGTGATGTAC
TATAAGAGTA AAGAAAATTG TGTTGTGGAT AACATCAAAG TGTGCAGTAA TGACACTGGG
AGTGGAAAAT TCAAGTGTGT TTGCATCACT ATGAGAGTGC CTCGGAACCC AACTATCGGA
GATAAATTTG CCAGTCGCCA TGGGCAGAAG GGCATTTTAA GCAGATTGTG GCCGGCTGAG
GACATGCCTT TTACTGAGAG TGGGATGGTC CCAGACATTC TGTTCAATCC CCATGGTTTT
CCATCCCGCA TGACCATTGG GATGTTAATT GAGAGTATGG CCGGGAAGTC TGCAGCTTTG
CATGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCAG AGGAGAACTC GGCCTTAGAA
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAATTTCT ATGGCACCGA GAGGTTATAT
AGTGGCATCA GTGGGCTAGA ACTGGAAGCA GACATCTTCA TAGGAGTGGT TTATTATCAG
CGCTTACGCC ATATGGTCTC AGACAAATTT CAAGTAAGGA CAACTGGAGC CCGAGACAGA
GTCACCAACC AGCCTATTGG GGGAAGAAAT GTCCAGGGTG GAATCCGTTT TGGGGAGATG
GAACGGGATG CGCTTTTAGC TCATGGTACA TCTTTTCTCC TTCATGACCG CCTCTTCAAC
TGCTCAGATC GGTCGGTAGC CCATGTGTGT GTGAAGTGTG GCAGTTTACT CTCTCCACTG
TTGGAGAAGC CACCCCCTTC TTGGTCTGCC ATGCGCAACA GAAAATACAA CTGTACTCTG
TGTAGTCGCA GTGACACTAT CGATACTGTT TCTGTGCCTT ATGTTTTTCG GTATTTTGTA
GCTGAACTGG CAGCTATGAA CATCAAAGTG AAACTGGATG TTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu08732 Clone ID Related Accession (Same CDS sequence) NM_019014.5 , NM_019014.6 Accession Version NM_019014.6 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1568390400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 1 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_019014.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.163661.1, SRR1803617.117567.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGGTGT TGAGAGAGAA AAGAATTCCT CCCTGGATGG AAGTGGTCCT TATACCCATG
ACAGGAAAAC CAAGTCTGTA CCCAGGATTG TTCCTTTTTA CCACTCCTTG TAGACTGGTA
CGGCCTGTGC AGAACTTAGC ATTGGGCAAA GAAGAGCTAA TTGGAACTAT GGAACAGATC
TTCATGAATG TCGCTATCTT TGAGGATGAA GTTTTTGCTG GAGTTACCAC ACACCAGGAA
CTCTTTCCAC ACAGCCTGCT GAGTGTGATT GCCAACTTCA TCCCTTTCTC TGATCACAAC
CAGAGTCCAC GGAACATGTA CCAATGCCAG ATGGGTAAGC AAACTATGGG CTTTCCACTT
CTCACTTATC AAGACCGATC GGATAACAAA CTGTATCGTC TTCAGACTCC TCAGAGTCCC
TTGGTGAGAC CCTCCATGTA TGATTATTAT GACATGGATA ACTATCCAAT TGGGACCAAT
GCCATCGTTG CTGTGATTTC TTACACTGGC TATGATATGG AAGATGCCAT GATTGTGAAT
AAGGCCTCTT GGGAACGAGG CTTTGCCCAT GGAAGTGTCT ACAAGTCTGA GTTCATAGAC
CTCTCTGAAA AAATTAAACA AGGAGATAGT AGCCTGGTGT TTGGCATCAA ACCTGGTGAC
CCACGCGTTC TGCAGAAGTT AGATGACGAT GGATTGCCGT TTATAGGAGC AAAACTGCAG
TACGGAGATC CGTATTACAG CTACCTCAAC CTCAACACCG GGGAAAGTTT TGTGATGTAC
TATAAGAGTA AAGAAAATTG TGTTGTGGAT AACATCAAAG TGTGCAGTAA TGACACTGGG
AGTGGAAAAT TCAAGTGTGT TTGCATCACT ATGAGAGTGC CTCGGAACCC AACTATCGGA
GATAAATTTG CCAGTCGCCA TGGGCAGAAG GGCATTTTAA GCAGATTGTG GCCGGCTGAG
GACATGCCTT TTACTGAGAG TGGGATGGTC CCAGACATTC TGTTCAATCC CCATGGTTTT
CCATCCCGCA TGACCATTGG GATGTTAATT GAGAGTATGG CCGGGAAGTC TGCAGCTTTG
CATGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCAG AGGAGAACTC GGCCTTAGAA
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAATTTCT ATGGCACCGA GAGGTTATAT
AGTGGCATCA GTGGGCTAGA ACTGGAAGCA GACATCTTCA TAGGAGTGGT TTATTATCAG
CGCTTACGCC ATATGGTCTC AGACAAATTT CAAGTAAGGA CAACTGGAGC CCGAGACAGA
GTCACCAACC AGCCTATTGG GGGAAGAAAT GTCCAGGGTG GAATCCGTTT TGGGGAGATG
GAACGGGATG CGCTTTTAGC TCATGGTACA TCTTTTCTCC TTCATGACCG CCTCTTCAAC
TGCTCAGATC GGTCGGTAGC CCATGTGTGT GTGAAGTGTG GCAGTTTACT CTCTCCACTG
TTGGAGAAGC CACCCCCTTC TTGGTCTGCC ATGCGCAACA GAAAATACAA CTGTACTCTG
TGTAGTCGCA GTGACACTAT CGATACTGTT TCTGTGCCTT ATGTTTTTCG GTATTTTGTA
GCTGAACTGG CAGCTATGAA CATCAAAGTG AAACTGGATG TTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_061887.2 CDS 31..3438 Translation
Target ORF information:
RefSeq Version NM_019014.6 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_019014.6
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGGTGT TGAGAGAGAA AAGAATTCCT CCCTGGATGG AAGTGGTCCT TATACCCATG
ACAGGAAAAC CAAGTCTGTA CCCAGGATTG TTCCTTTTTA CCACTCCTTG TAGACTGGTA
CGGCCTGTGC AGAACTTAGC ATTGGGCAAA GAAGAGCTAA TTGGAACTAT GGAACAGATC
TTCATGAATG TCGCTATCTT TGAGGATGAA GTTTTTGCTG GAGTTACCAC ACACCAGGAA
CTCTTTCCAC ACAGCCTGCT GAGTGTGATT GCCAACTTCA TCCCTTTCTC TGATCACAAC
CAGAGTCCAC GGAACATGTA CCAATGCCAG ATGGGTAAGC AAACTATGGG CTTTCCACTT
CTCACTTATC AAGACCGATC GGATAACAAA CTGTATCGTC TTCAGACTCC TCAGAGTCCC
TTGGTGAGAC CCTCCATGTA TGATTATTAT GACATGGATA ACTATCCAAT TGGGACCAAT
GCCATCGTTG CTGTGATTTC TTACACTGGC TATGATATGG AAGATGCCAT GATTGTGAAT
AAGGCCTCTT GGGAACGAGG CTTTGCCCAT GGAAGTGTCT ACAAGTCTGA GTTCATAGAC
CTCTCTGAAA AAATTAAACA AGGAGATAGT AGCCTGGTGT TTGGCATCAA ACCTGGTGAC
CCACGCGTTC TGCAGAAGTT AGATGACGAT GGATTGCCGT TTATAGGAGC AAAACTGCAG
TACGGAGATC CGTATTACAG CTACCTCAAC CTCAACACCG GGGAAAGTTT TGTGATGTAC
TATAAGAGTA AAGAAAATTG TGTTGTGGAT AACATCAAAG TGTGCAGTAA TGACACTGGG
AGTGGAAAAT TCAAGTGTGT TTGCATCACT ATGAGAGTGC CTCGGAACCC AACTATCGGA
GATAAATTTG CCAGTCGCCA TGGGCAGAAG GGCATTTTAA GCAGATTGTG GCCGGCTGAG
GACATGCCTT TTACTGAGAG TGGGATGGTC CCAGACATTC TGTTCAATCC CCATGGTTTT
CCATCCCGCA TGACCATTGG GATGTTAATT GAGAGTATGG CCGGGAAGTC TGCAGCTTTG
CATGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCAG AGGAGAACTC GGCCTTAGAA
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAATTTCT ATGGCACCGA GAGGTTATAT
AGTGGCATCA GTGGGCTAGA ACTGGAAGCA GACATCTTCA TAGGAGTGGT TTATTATCAG
CGCTTACGCC ATATGGTCTC AGACAAATTT CAAGTAAGGA CAACTGGAGC CCGAGACAGA
GTCACCAACC AGCCTATTGG GGGAAGAAAT GTCCAGGGTG GAATCCGTTT TGGGGAGATG
GAACGGGATG CGCTTTTAGC TCATGGTACA TCTTTTCTCC TTCATGACCG CCTCTTCAAC
TGCTCAGATC GGTCGGTAGC CCATGTGTGT GTGAAGTGTG GCAGTTTACT CTCTCCACTG
TTGGAGAAGC CACCCCCTTC TTGGTCTGCC ATGCGCAACA GAAAATACAA CTGTACTCTG
TGTAGTCGCA GTGACACTAT CGATACTGTT TCTGTGCCTT ATGTTTTTCG GTATTTTGTA
GCTGAACTGG CAGCTATGAA CATCAAAGTG AAACTGGATG TTGTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu108856 Clone ID Related Accession (Same CDS sequence) NM_001371969.1 Accession Version NM_001371969.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 7 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.128576.1 [ECO:0000332] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGAGTC TCGCTCTGTC GCCCAGGCTG CAGTGCAGTG GTGTGATCTT GGCTCACTGC
AGCCTCCGCC TCCCATGTTC AAGCAATTCT CCTGCCTCGG TCTCCCAAGT AGCTGGGATT
ACAGGTGCAC ACCACCATGC CTGGGTGTTG AGAGAGAAAA GAATTCCTCC CTGGATGGAA
GTGGTCCTTA TACCCATGAC AGGAAAACCA AGTCTGTACC CAGGATTGTT CCTTTTTACC
ACTCCTTGTA GACTGGTACG GCCTGTGCAG AACTTAGCAT TGGGCAAAGA AGAGCTAATT
GGAACTATGG AACAGATCTT CATGAATGTC GCTATCTTTG AGGATGAAGT TTTTGCTGGA
GTTACCACAC ACCAGGAACT CTTTCCACAC AGCCTGCTGA GTGTGATTGC CAACTTCATC
CCTTTCTCTG ATCACAACCA GAGTCCACGG AACATGTACC AATGCCAGAT GGGTAAGCAA
ACTATGGGCT TTCCACTTCT CACTTATCAA GACCGATCGG ATAACAAACT GTATCGTCTT
CAGACTCCTC AGAGTCCCTT GGTGAGACCC TCCATGTATG ATTATTATGA CATGGATAAC
TATCCAATTG GGACCAATGC CATCGTTGCT GTGATTTCTT ACACTGGCTA TGATATGGAA
GATGCCATGA TTGTGAATAA GGCCTCTTGG GAACGAGGCT TTGCCCATGG AAGTGTCTAC
AAGTCTGAGT TCATAGACCT CTCTGAAAAA ATTAAACAAG GAGATAGTAG CCTGGTGTTT
GGCATCAAAC CTGGTGACCC ACGCGTTCTG CAGAAGTTAG ATGACGATGG ATTGCCGTTT
ATAGGAGCAA AACTGCAGTA CGGAGATCCG TATTACAGCT ACCTCAACCT CAACACCGGG
GAAAGTTTTG TGATGTACTA TAAGAGTAAA GAAAATTGTG TTGTGGATAA CATCAAAGTG
TGCAGTAATG ACACTGGGAG TGGAAAATTC AAGTGTGTTT GCATCACTAT GAGAGTGCCT
CGGAACCCAA CTATCGGAGA TAAATTTGCC AGTCGCCATG GGCAGAAGGG CATTTTAAGC
AGATTGTGGC CGGCTGAGGA CATGCCTTTT ACTGAGAGTG GGATGGTCCC AGACATTCTG
TTCAATCCCC ATGGTTTTCC ATCCCGCATG ACCATTGGGA TGTTAATTGA GAGTATGGCC
GGGAAGTCTG CAGCTTTGCA TGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCAGAG
GAGAACTCGG CCTTAGAATA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAATTTCTAT
GGCACCGAGA GGTTATATAG TGGCATCAGT GGGCTAGAAC TGGAAGCAGA CATCTTCATA
GGAGTGGTTT ATTATCAGCG CTTACGCCAT ATGGTCTCAG ACAAATTTCA AGTAAGGACA
ACTGGAGCCC GAGACAGAGT CACCAACCAG CCTATTGGGG GAAGAAATGT CCAGGGTGGA
ATCCGTTTTG GGGAGATGGA ACGGGATGCG CTTTTAGCTC ATGGTACATC TTTTCTCCTT
CATGACCGCC TCTTCAACTG CTCAGATCGG TCGGTAGCCC ATGTGTGTGT GAAGTGTGGC
AGTTTACTCT CTCCACTGTT GGAGAAGCCA CCCCCTTCTT GGTCTGCCAT GCGCAACAGA
AAATACAACT GTACTCTGTG TAGTCGCAGT GACACTATCG ATACTGTTTC TGTGCCTTAT
GTTTTTCGGT ATTTTGTAGC TGAACTGGCA GCTATGAACA TCAAAGTGAA ACTGGATGTT
GTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001358898.1 CDS 31..3576 Translation
Target ORF information:
RefSeq Version NM_001371969.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 8, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001371969.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541ATGGATCCTG GCAGCCGGTG GCGGAACCTG CCCAGCGGGC CTAGCCTAAA GCACTTGACT
GACCCCTCTT ATGGAATCCC GCGGGAACAG CAAAAGGCAG CGTTGCAGGA GCTGACGCGG
GCGCACGTGG AGTCCTTCAA CTACGCTGTG CACGAGGGTC TCGGCCTCGC GGTGCAGGCT
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGTATCT CTTTTACTAT TCTGGATGCT
GTTATCAGTC CACCTACAGT TCCAAAAGGG ACCATCTGCA AAGAGGCCAA TGTTTATCCA
GCAGAATGCC GGGGCCGAAG GAGTACCTAC CGTGGGAAGT TGACAGCTGA TATCAACTGG
GCAGTGAATG GAATCTCAAA AGGAATCATT AAGCAGTTTC TTGGCTATGT TCCCATCATG
GTGAAATCCA AGCTTTGCAA CTTACGTAAC CTTCCCCCAC AAGCCCTCAT TGAGCACCAT
GAGGAGGCAG AGGAAATGGG GGGCTATTTT ATAATCAATG GCATTGAAAA AGTCATCCGA
ATGTTGATTA TGCCTCGGAG AAATTTTCCC ATTGCAATGA TAAGACCAAA ATGGAAAACC
AGAGGGCCTG GTTATACTCA GTATGGAGTT TCAATGCACT GTGTGAGGGA AGAACATTCC
GCTGTCAATA TGAACCTCCA CTACTTGGAA AATGGCACTG TTATGTTGAA CTTTATTTAC
CGAAAAGAAC TGTTCTTTCT TCCTTTGGGA TTTGCACTTA AGGCACTTGT CAGCTTTTCT
GATTATCAGA TCTTTCAGGA GCTCATCAAA GGAAAAGAGG ATGATTCTTT CCTTAGGAAC
TCTGTTTCTC AGATGTTAAG GATTGTAATG GAAGAGGGTT GTTCGACACA AAAACAGGTC
CTTAACTACC TAGGTGAATG CTTCAGAGTA AAACTCAATG TTCCTGACTG GTACCCAAAT
GAGCAAGCTG CGGAGTTCCT GTTTAACCAG TGCATCTGTA TCCACTTGAA ATCCAATACT
GAAAAGTTTT ATATGCTTTG TCTCATGACG CGAAAGCTCT TTGCTTTAGC CAAAGGAGAG
TGCATGGAGG ACAATCCTGA TAGTTTGGTG AACCAGGAAG TCCTCACACC GGGTCAGCTC
TTCCTTATGT TCCTGAAGGA AAAACTGGAA GGTTGGTTAG TGTCTATTAA AATAGCTTTT
GATAAGAAGG CTCAGAAGAC CAGTGTTTCC ATGAACACTG ACAATTTGAT GAGGATTTTT
ACAATGGGCA TAGACCTTAC AAAACCATTT GAATACCTTT TTGCTACTGG GAATCTGCGT
TCTAAAACAG GTCTTGGCCT CCTACAAGAT TCTGGACTTT GTGTTGTGGC TGACAAGCTG
AACTTCATAC GCTACCTCTC CCATTTCCGC TGCGTGCACA GAGGGGCTGA TTTTGCCAAG
ATGAGGACCA CCACAGTACG CAGGCTGCTG CCAGAGTCCT GGGGCTTCCT TTGTCCCGTG
CATACCCCAG ACGGGGAGCC CTGTGGCCTG ATGAACCACC TAACTGCCGT ATGTGAGGTT
GTCACACAGT TTGTGTATAC GGCATCTATT CCAGCTTTAC TGTGCAACTT GGGGGTCACT
CCCATTGATG GAGCTCCCCA CCGATCATAC AGTGAGTGCT ACCCTGTCCT GCTGGACGGT
GTCATGGTTG GCTGGGTGGA TAAGGATCTT GCTCCAGGCA TCGCAGATTC TCTTCGTCAT
TTTAAGAGTC TCGCTCTGTC GCCCAGGCTG CAGTGCAGTG GTGTGATCTT GGCTCACTGC
AGCCTCCGCC TCCCATGTTC AAGCAATTCT CCTGCCTCGG TCTCCCAAGT AGCTGGGATT
ACAGGTGCAC ACCACCATGC CTGGGTGTTG AGAGAGAAAA GAATTCCTCC CTGGATGGAA
GTGGTCCTTA TACCCATGAC AGGAAAACCA AGTCTGTACC CAGGATTGTT CCTTTTTACC
ACTCCTTGTA GACTGGTACG GCCTGTGCAG AACTTAGCAT TGGGCAAAGA AGAGCTAATT
GGAACTATGG AACAGATCTT CATGAATGTC GCTATCTTTG AGGATGAAGT TTTTGCTGGA
GTTACCACAC ACCAGGAACT CTTTCCACAC AGCCTGCTGA GTGTGATTGC CAACTTCATC
CCTTTCTCTG ATCACAACCA GAGTCCACGG AACATGTACC AATGCCAGAT GGGTAAGCAA
ACTATGGGCT TTCCACTTCT CACTTATCAA GACCGATCGG ATAACAAACT GTATCGTCTT
CAGACTCCTC AGAGTCCCTT GGTGAGACCC TCCATGTATG ATTATTATGA CATGGATAAC
TATCCAATTG GGACCAATGC CATCGTTGCT GTGATTTCTT ACACTGGCTA TGATATGGAA
GATGCCATGA TTGTGAATAA GGCCTCTTGG GAACGAGGCT TTGCCCATGG AAGTGTCTAC
AAGTCTGAGT TCATAGACCT CTCTGAAAAA ATTAAACAAG GAGATAGTAG CCTGGTGTTT
GGCATCAAAC CTGGTGACCC ACGCGTTCTG CAGAAGTTAG ATGACGATGG ATTGCCGTTT
ATAGGAGCAA AACTGCAGTA CGGAGATCCG TATTACAGCT ACCTCAACCT CAACACCGGG
GAAAGTTTTG TGATGTACTA TAAGAGTAAA GAAAATTGTG TTGTGGATAA CATCAAAGTG
TGCAGTAATG ACACTGGGAG TGGAAAATTC AAGTGTGTTT GCATCACTAT GAGAGTGCCT
CGGAACCCAA CTATCGGAGA TAAATTTGCC AGTCGCCATG GGCAGAAGGG CATTTTAAGC
AGATTGTGGC CGGCTGAGGA CATGCCTTTT ACTGAGAGTG GGATGGTCCC AGACATTCTG
TTCAATCCCC ATGGTTTTCC ATCCCGCATG ACCATTGGGA TGTTAATTGA GAGTATGGCC
GGGAAGTCTG CAGCTTTGCA TGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCAGAG
GAGAACTCGG CCTTAGAATA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAATTTCTAT
GGCACCGAGA GGTTATATAG TGGCATCAGT GGGCTAGAAC TGGAAGCAGA CATCTTCATA
GGAGTGGTTT ATTATCAGCG CTTACGCCAT ATGGTCTCAG ACAAATTTCA AGTAAGGACA
ACTGGAGCCC GAGACAGAGT CACCAACCAG CCTATTGGGG GAAGAAATGT CCAGGGTGGA
ATCCGTTTTG GGGAGATGGA ACGGGATGCG CTTTTAGCTC ATGGTACATC TTTTCTCCTT
CATGACCGCC TCTTCAACTG CTCAGATCGG TCGGTAGCCC ATGTGTGTGT GAAGTGTGGC
AGTTTACTCT CTCCACTGTT GGAGAAGCCA CCCCCTTCTT GGTCTGCCAT GCGCAACAGA
AAATACAACT GTACTCTGTG TAGTCGCAGT GACACTATCG ATACTGTTTC TGTGCCTTAT
GTTTTTCGGT ATTTTGTAGC TGAACTGGCA GCTATGAACA TCAAAGTGAA ACTGGATGTT
GTTTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu11089 Clone ID Related Accession (Same CDS sequence) NM_001282772.1 , NM_001282772.2 Accession Version NM_001282772.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1530201600000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 3 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK302814.1, AC012442.7 and AA484631.1. On Sep 19, 2013 this sequence version replaced XM_005263813.1. Transcript Variant: This variant (3) contains alternate 5' exon structure and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481ATGAGAGCCA AAGAGGCTGC GGAAACGGGA CTGCGGCCAC TACTTCCGGC GTGTACCGAG
AGACTGGCGT CCGGCGTGTA CCGAGAGACT GGCGTCCGGT GTGCAGGTGG CCACATGGAT
CCTGGCAGCC GGTGGCGGAA CCTGCCCAGC GGGCCTAGCC TAAAGCACTT GACTGACCCC
TCTTATGGAA TCCCGCGGGA ACAGCAAAAG GCAGCGTTGC AGGAGCTGAC GCGGGCGCAC
GTGGAGTCCT TCAACTACGC TGTGCACGAG GGTCTCGGCC TCGCGGTGCA GGCTATACCT
CCCTTTGAAT TTGCTTTCAA AGATGAGCGT ATCTCTTTTA CTATTCTGGA TGCTGTTATC
AGTCCACCTA CAGTTCCAAA AGGGACCATC TGCAAAGAGG CCAATGTTTA TCCAGCAGAA
TGCCGGGGCC GAAGGAGTAC CTACCGTGGG AAGTTGACAG CTGATATCAA CTGGGCAGTG
AATGGAATCT CAAAAGGAAT CATTAAGCAG TTTCTTGGCT ATGTTCCCAT CATGGTGAAA
TCCAAGCTTT GCAACTTACG TAACCTTCCC CCACAAGCCC TCATTGAGCA CCATGAGGAG
GCAGAGGAAA TGGGGGGCTA TTTTATAATC AATGGCATTG AAAAAGTCAT CCGAATGTTG
ATTATGCCTC GGAGAAATTT TCCCATTGCA ATGATAAGAC CAAAATGGAA AACCAGAGGG
CCTGGTTATA CTCAGTATGG AGTTTCAATG CACTGTGTGA GGGAAGAACA TTCCGCTGTC
AATATGAACC TCCACTACTT GGAAAATGGC ACTGTTATGT TGAACTTTAT TTACCGAAAA
GAACTGTTCT TTCTTCCTTT GGGATTTGCA CTTAAGGCAC TTGTCAGCTT TTCTGATTAT
CAGATCTTTC AGGAGCTCAT CAAAGGAAAA GAGGATGATT CTTTCCTTAG GAACTCTGTT
TCTCAGATGT TAAGGATTGT AATGGAAGAG GGTTGTTCGA CACAAAAACA GGTCCTTAAC
TACCTAGGTG AATGCTTCAG AGTAAAACTC AATGTTCCTG ACTGGTACCC AAATGAGCAA
GCTGCGGAGT TCCTGTTTAA CCAGTGCATC TGTATCCACT TGAAATCCAA TACTGAAAAG
TTTTATATGC TTTGTCTCAT GACGCGAAAG CTCTTTGCTT TAGCCAAAGG AGAGTGCATG
GAGGACAATC CTGATAGTTT GGTGAACCAG GAAGTCCTCA CACCGGGTCA GCTCTTCCTT
ATGTTCCTGA AGGAAAAACT GGAAGGTTGG TTAGTGTCTA TTAAAATAGC TTTTGATAAG
AAGGCTCAGA AGACCAGTGT TTCCATGAAC ACTGACAATT TGATGAGGAT TTTTACAATG
GGCATAGACC TTACAAAACC ATTTGAATAC CTTTTTGCTA CTGGGAATCT GCGTTCTAAA
ACAGGTCTTG GCCTCCTACA AGATTCTGGA CTTTGTGTTG TGGCTGACAA GCTGAACTTC
ATACGCTACC TCTCCCATTT CCGCTGCGTG CACAGAGGGG CTGATTTTGC CAAGATGAGG
ACCACCACAG TACGCAGGCT GCTGCCAGAG TCCTGGGGCT TCCTTTGTCC CGTGCATACC
CCAGACGGGG AGCCCTGTGG CCTGATGAAC CACCTAACTG CCGTATGTGA GGTTGTCACA
CAGTTTGTGT ATACGGCATC TATTCCAGCT TTACTGTGCA ACTTGGGGGT CACTCCCATT
GATGGAGCTC CCCACCGATC ATACAGTGAG TGCTACCCTG TCCTGCTGGA CGGTGTCATG
GTTGGCTGGG TGGATAAGGA TCTTGCTCCA GGCATCGCAG ATTCTCTTCG TCATTTTAAG
GTGTTGAGAG AGAAAAGAAT TCCTCCCTGG ATGGAAGTGG TCCTTATACC CATGACAGGA
AAACCAAGTC TGTACCCAGG ATTGTTCCTT TTTACCACTC CTTGTAGACT GGTACGGCCT
GTGCAGAACT TAGCATTGGG CAAAGAAGAG CTAATTGGAA CTATGGAACA GATCTTCATG
AATGTCGCTA TCTTTGAGGA TGAAGTTTTT GCTGGAGTTA CCACACACCA GGAACTCTTT
CCACACAGCC TGCTGAGTGT GATTGCCAAC TTCATCCCTT TCTCTGATCA CAACCAGAGT
CCACGGAACA TGTACCAATG CCAGATGGGT AAGCAAACTA TGGGCTTTCC ACTTCTCACT
TATCAAGACC GATCGGATAA CAAACTGTAT CGTCTTCAGA CTCCTCAGAG TCCCTTGGTG
AGACCCTCCA TGTATGATTA TTATGACATG GATAACTATC CAATTGGGAC CAATGCCATC
GTTGCTGTGA TTTCTTACAC TGGCTATGAT ATGGAAGATG CCATGATTGT GAATAAGGCC
TCTTGGGAAC GAGGCTTTGC CCATGGAAGT GTCTACAAGT CTGAGTTCAT AGACCTCTCT
GAAAAAATTA AACAAGGAGA TAGTAGCCTG GTGTTTGGCA TCAAACCTGG TGACCCACGC
GTTCTGCAGA AGTTAGATGA CGATGGATTG CCGTTTATAG GAGCAAAACT GCAGTACGGA
GATCCGTATT ACAGCTACCT CAACCTCAAC ACCGGGGAAA GTTTTGTGAT GTACTATAAG
AGTAAAGAAA ATTGTGTTGT GGATAACATC AAAGTGTGCA GTAATGACAC TGGGAGTGGA
AAATTCAAGT GTGTTTGCAT CACTATGAGA GTGCCTCGGA ACCCAACTAT CGGAGATAAA
TTTGCCAGTC GCCATGGGCA GAAGGGCATT TTAAGCAGAT TGTGGCCGGC TGAGGACATG
CCTTTTACTG AGAGTGGGAT GGTCCCAGAC ATTCTGTTCA ATCCCCATGG TTTTCCATCC
CGCATGACCA TTGGGATGTT AATTGAGAGT ATGGCCGGGA AGTCTGCAGC TTTGCATGGT
CTCTGCCATG ATGCTACACC CTTCATCTTC TCAGAGGAGA ACTCGGCCTT AGAATACTTT
GGTGAGATGT TAAAGGCTGC TGGCTACAAT TTCTATGGCA CCGAGAGGTT ATATAGTGGC
ATCAGTGGGC TAGAACTGGA AGCAGACATC TTCATAGGAG TGGTTTATTA TCAGCGCTTA
CGCCATATGG TCTCAGACAA ATTTCAAGTA AGGACAACTG GAGCCCGAGA CAGAGTCACC
AACCAGCCTA TTGGGGGAAG AAATGTCCAG GGTGGAATCC GTTTTGGGGA GATGGAACGG
GATGCGCTTT TAGCTCATGG TACATCTTTT CTCCTTCATG ACCGCCTCTT CAACTGCTCA
GATCGGTCGG TAGCCCATGT GTGTGTGAAG TGTGGCAGTT TACTCTCTCC ACTGTTGGAG
AAGCCACCCC CTTCTTGGTC TGCCATGCGC AACAGAAAAT ACAACTGTAC TCTGTGTAGT
CGCAGTGACA CTATCGATAC TGTTTCTGTG CCTTATGTTT TTCGGTATTT TGTAGCTGAA
CTGGCAGCTA TGAACATCAA AGTGAAACTG GATGTTGTTT AAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269701.1 CDS 117..3638 Translation
Target ORF information:
RefSeq Version NM_001282772.1 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282772.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481ATGAGAGCCA AAGAGGCTGC GGAAACGGGA CTGCGGCCAC TACTTCCGGC GTGTACCGAG
AGACTGGCGT CCGGCGTGTA CCGAGAGACT GGCGTCCGGT GTGCAGGTGG CCACATGGAT
CCTGGCAGCC GGTGGCGGAA CCTGCCCAGC GGGCCTAGCC TAAAGCACTT GACTGACCCC
TCTTATGGAA TCCCGCGGGA ACAGCAAAAG GCAGCGTTGC AGGAGCTGAC GCGGGCGCAC
GTGGAGTCCT TCAACTACGC TGTGCACGAG GGTCTCGGCC TCGCGGTGCA GGCTATACCT
CCCTTTGAAT TTGCTTTCAA AGATGAGCGT ATCTCTTTTA CTATTCTGGA TGCTGTTATC
AGTCCACCTA CAGTTCCAAA AGGGACCATC TGCAAAGAGG CCAATGTTTA TCCAGCAGAA
TGCCGGGGCC GAAGGAGTAC CTACCGTGGG AAGTTGACAG CTGATATCAA CTGGGCAGTG
AATGGAATCT CAAAAGGAAT CATTAAGCAG TTTCTTGGCT ATGTTCCCAT CATGGTGAAA
TCCAAGCTTT GCAACTTACG TAACCTTCCC CCACAAGCCC TCATTGAGCA CCATGAGGAG
GCAGAGGAAA TGGGGGGCTA TTTTATAATC AATGGCATTG AAAAAGTCAT CCGAATGTTG
ATTATGCCTC GGAGAAATTT TCCCATTGCA ATGATAAGAC CAAAATGGAA AACCAGAGGG
CCTGGTTATA CTCAGTATGG AGTTTCAATG CACTGTGTGA GGGAAGAACA TTCCGCTGTC
AATATGAACC TCCACTACTT GGAAAATGGC ACTGTTATGT TGAACTTTAT TTACCGAAAA
GAACTGTTCT TTCTTCCTTT GGGATTTGCA CTTAAGGCAC TTGTCAGCTT TTCTGATTAT
CAGATCTTTC AGGAGCTCAT CAAAGGAAAA GAGGATGATT CTTTCCTTAG GAACTCTGTT
TCTCAGATGT TAAGGATTGT AATGGAAGAG GGTTGTTCGA CACAAAAACA GGTCCTTAAC
TACCTAGGTG AATGCTTCAG AGTAAAACTC AATGTTCCTG ACTGGTACCC AAATGAGCAA
GCTGCGGAGT TCCTGTTTAA CCAGTGCATC TGTATCCACT TGAAATCCAA TACTGAAAAG
TTTTATATGC TTTGTCTCAT GACGCGAAAG CTCTTTGCTT TAGCCAAAGG AGAGTGCATG
GAGGACAATC CTGATAGTTT GGTGAACCAG GAAGTCCTCA CACCGGGTCA GCTCTTCCTT
ATGTTCCTGA AGGAAAAACT GGAAGGTTGG TTAGTGTCTA TTAAAATAGC TTTTGATAAG
AAGGCTCAGA AGACCAGTGT TTCCATGAAC ACTGACAATT TGATGAGGAT TTTTACAATG
GGCATAGACC TTACAAAACC ATTTGAATAC CTTTTTGCTA CTGGGAATCT GCGTTCTAAA
ACAGGTCTTG GCCTCCTACA AGATTCTGGA CTTTGTGTTG TGGCTGACAA GCTGAACTTC
ATACGCTACC TCTCCCATTT CCGCTGCGTG CACAGAGGGG CTGATTTTGC CAAGATGAGG
ACCACCACAG TACGCAGGCT GCTGCCAGAG TCCTGGGGCT TCCTTTGTCC CGTGCATACC
CCAGACGGGG AGCCCTGTGG CCTGATGAAC CACCTAACTG CCGTATGTGA GGTTGTCACA
CAGTTTGTGT ATACGGCATC TATTCCAGCT TTACTGTGCA ACTTGGGGGT CACTCCCATT
GATGGAGCTC CCCACCGATC ATACAGTGAG TGCTACCCTG TCCTGCTGGA CGGTGTCATG
GTTGGCTGGG TGGATAAGGA TCTTGCTCCA GGCATCGCAG ATTCTCTTCG TCATTTTAAG
GTGTTGAGAG AGAAAAGAAT TCCTCCCTGG ATGGAAGTGG TCCTTATACC CATGACAGGA
AAACCAAGTC TGTACCCAGG ATTGTTCCTT TTTACCACTC CTTGTAGACT GGTACGGCCT
GTGCAGAACT TAGCATTGGG CAAAGAAGAG CTAATTGGAA CTATGGAACA GATCTTCATG
AATGTCGCTA TCTTTGAGGA TGAAGTTTTT GCTGGAGTTA CCACACACCA GGAACTCTTT
CCACACAGCC TGCTGAGTGT GATTGCCAAC TTCATCCCTT TCTCTGATCA CAACCAGAGT
CCACGGAACA TGTACCAATG CCAGATGGGT AAGCAAACTA TGGGCTTTCC ACTTCTCACT
TATCAAGACC GATCGGATAA CAAACTGTAT CGTCTTCAGA CTCCTCAGAG TCCCTTGGTG
AGACCCTCCA TGTATGATTA TTATGACATG GATAACTATC CAATTGGGAC CAATGCCATC
GTTGCTGTGA TTTCTTACAC TGGCTATGAT ATGGAAGATG CCATGATTGT GAATAAGGCC
TCTTGGGAAC GAGGCTTTGC CCATGGAAGT GTCTACAAGT CTGAGTTCAT AGACCTCTCT
GAAAAAATTA AACAAGGAGA TAGTAGCCTG GTGTTTGGCA TCAAACCTGG TGACCCACGC
GTTCTGCAGA AGTTAGATGA CGATGGATTG CCGTTTATAG GAGCAAAACT GCAGTACGGA
GATCCGTATT ACAGCTACCT CAACCTCAAC ACCGGGGAAA GTTTTGTGAT GTACTATAAG
AGTAAAGAAA ATTGTGTTGT GGATAACATC AAAGTGTGCA GTAATGACAC TGGGAGTGGA
AAATTCAAGT GTGTTTGCAT CACTATGAGA GTGCCTCGGA ACCCAACTAT CGGAGATAAA
TTTGCCAGTC GCCATGGGCA GAAGGGCATT TTAAGCAGAT TGTGGCCGGC TGAGGACATG
CCTTTTACTG AGAGTGGGAT GGTCCCAGAC ATTCTGTTCA ATCCCCATGG TTTTCCATCC
CGCATGACCA TTGGGATGTT AATTGAGAGT ATGGCCGGGA AGTCTGCAGC TTTGCATGGT
CTCTGCCATG ATGCTACACC CTTCATCTTC TCAGAGGAGA ACTCGGCCTT AGAATACTTT
GGTGAGATGT TAAAGGCTGC TGGCTACAAT TTCTATGGCA CCGAGAGGTT ATATAGTGGC
ATCAGTGGGC TAGAACTGGA AGCAGACATC TTCATAGGAG TGGTTTATTA TCAGCGCTTA
CGCCATATGG TCTCAGACAA ATTTCAAGTA AGGACAACTG GAGCCCGAGA CAGAGTCACC
AACCAGCCTA TTGGGGGAAG AAATGTCCAG GGTGGAATCC GTTTTGGGGA GATGGAACGG
GATGCGCTTT TAGCTCATGG TACATCTTTT CTCCTTCATG ACCGCCTCTT CAACTGCTCA
GATCGGTCGG TAGCCCATGT GTGTGTGAAG TGTGGCAGTT TACTCTCTCC ACTGTTGGAG
AAGCCACCCC CTTCTTGGTC TGCCATGCGC AACAGAAAAT ACAACTGTAC TCTGTGTAGT
CGCAGTGACA CTATCGATAC TGTTTCTGTG CCTTATGTTT TTCGGTATTT TGTAGCTGAA
CTGGCAGCTA TGAACATCAA AGTGAAACTG GATGTTGTTT AAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu11089 Clone ID Related Accession (Same CDS sequence) NM_001282772.1 , NM_001282772.2 Accession Version NM_001282772.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569686400000 Organism Homo sapiens(human) Product DNA-directed RNA polymerase I subunit RPA2 isoform 3 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012442.7. On Jul 31, 2019 this sequence version replaced NM_001282772.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302814.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481ATGAGAGCCA AAGAGGCTGC GGAAACGGGA CTGCGGCCAC TACTTCCGGC GTGTACCGAG
AGACTGGCGT CCGGCGTGTA CCGAGAGACT GGCGTCCGGT GTGCAGGTGG CCACATGGAT
CCTGGCAGCC GGTGGCGGAA CCTGCCCAGC GGGCCTAGCC TAAAGCACTT GACTGACCCC
TCTTATGGAA TCCCGCGGGA ACAGCAAAAG GCAGCGTTGC AGGAGCTGAC GCGGGCGCAC
GTGGAGTCCT TCAACTACGC TGTGCACGAG GGTCTCGGCC TCGCGGTGCA GGCTATACCT
CCCTTTGAAT TTGCTTTCAA AGATGAGCGT ATCTCTTTTA CTATTCTGGA TGCTGTTATC
AGTCCACCTA CAGTTCCAAA AGGGACCATC TGCAAAGAGG CCAATGTTTA TCCAGCAGAA
TGCCGGGGCC GAAGGAGTAC CTACCGTGGG AAGTTGACAG CTGATATCAA CTGGGCAGTG
AATGGAATCT CAAAAGGAAT CATTAAGCAG TTTCTTGGCT ATGTTCCCAT CATGGTGAAA
TCCAAGCTTT GCAACTTACG TAACCTTCCC CCACAAGCCC TCATTGAGCA CCATGAGGAG
GCAGAGGAAA TGGGGGGCTA TTTTATAATC AATGGCATTG AAAAAGTCAT CCGAATGTTG
ATTATGCCTC GGAGAAATTT TCCCATTGCA ATGATAAGAC CAAAATGGAA AACCAGAGGG
CCTGGTTATA CTCAGTATGG AGTTTCAATG CACTGTGTGA GGGAAGAACA TTCCGCTGTC
AATATGAACC TCCACTACTT GGAAAATGGC ACTGTTATGT TGAACTTTAT TTACCGAAAA
GAACTGTTCT TTCTTCCTTT GGGATTTGCA CTTAAGGCAC TTGTCAGCTT TTCTGATTAT
CAGATCTTTC AGGAGCTCAT CAAAGGAAAA GAGGATGATT CTTTCCTTAG GAACTCTGTT
TCTCAGATGT TAAGGATTGT AATGGAAGAG GGTTGTTCGA CACAAAAACA GGTCCTTAAC
TACCTAGGTG AATGCTTCAG AGTAAAACTC AATGTTCCTG ACTGGTACCC AAATGAGCAA
GCTGCGGAGT TCCTGTTTAA CCAGTGCATC TGTATCCACT TGAAATCCAA TACTGAAAAG
TTTTATATGC TTTGTCTCAT GACGCGAAAG CTCTTTGCTT TAGCCAAAGG AGAGTGCATG
GAGGACAATC CTGATAGTTT GGTGAACCAG GAAGTCCTCA CACCGGGTCA GCTCTTCCTT
ATGTTCCTGA AGGAAAAACT GGAAGGTTGG TTAGTGTCTA TTAAAATAGC TTTTGATAAG
AAGGCTCAGA AGACCAGTGT TTCCATGAAC ACTGACAATT TGATGAGGAT TTTTACAATG
GGCATAGACC TTACAAAACC ATTTGAATAC CTTTTTGCTA CTGGGAATCT GCGTTCTAAA
ACAGGTCTTG GCCTCCTACA AGATTCTGGA CTTTGTGTTG TGGCTGACAA GCTGAACTTC
ATACGCTACC TCTCCCATTT CCGCTGCGTG CACAGAGGGG CTGATTTTGC CAAGATGAGG
ACCACCACAG TACGCAGGCT GCTGCCAGAG TCCTGGGGCT TCCTTTGTCC CGTGCATACC
CCAGACGGGG AGCCCTGTGG CCTGATGAAC CACCTAACTG CCGTATGTGA GGTTGTCACA
CAGTTTGTGT ATACGGCATC TATTCCAGCT TTACTGTGCA ACTTGGGGGT CACTCCCATT
GATGGAGCTC CCCACCGATC ATACAGTGAG TGCTACCCTG TCCTGCTGGA CGGTGTCATG
GTTGGCTGGG TGGATAAGGA TCTTGCTCCA GGCATCGCAG ATTCTCTTCG TCATTTTAAG
GTGTTGAGAG AGAAAAGAAT TCCTCCCTGG ATGGAAGTGG TCCTTATACC CATGACAGGA
AAACCAAGTC TGTACCCAGG ATTGTTCCTT TTTACCACTC CTTGTAGACT GGTACGGCCT
GTGCAGAACT TAGCATTGGG CAAAGAAGAG CTAATTGGAA CTATGGAACA GATCTTCATG
AATGTCGCTA TCTTTGAGGA TGAAGTTTTT GCTGGAGTTA CCACACACCA GGAACTCTTT
CCACACAGCC TGCTGAGTGT GATTGCCAAC TTCATCCCTT TCTCTGATCA CAACCAGAGT
CCACGGAACA TGTACCAATG CCAGATGGGT AAGCAAACTA TGGGCTTTCC ACTTCTCACT
TATCAAGACC GATCGGATAA CAAACTGTAT CGTCTTCAGA CTCCTCAGAG TCCCTTGGTG
AGACCCTCCA TGTATGATTA TTATGACATG GATAACTATC CAATTGGGAC CAATGCCATC
GTTGCTGTGA TTTCTTACAC TGGCTATGAT ATGGAAGATG CCATGATTGT GAATAAGGCC
TCTTGGGAAC GAGGCTTTGC CCATGGAAGT GTCTACAAGT CTGAGTTCAT AGACCTCTCT
GAAAAAATTA AACAAGGAGA TAGTAGCCTG GTGTTTGGCA TCAAACCTGG TGACCCACGC
GTTCTGCAGA AGTTAGATGA CGATGGATTG CCGTTTATAG GAGCAAAACT GCAGTACGGA
GATCCGTATT ACAGCTACCT CAACCTCAAC ACCGGGGAAA GTTTTGTGAT GTACTATAAG
AGTAAAGAAA ATTGTGTTGT GGATAACATC AAAGTGTGCA GTAATGACAC TGGGAGTGGA
AAATTCAAGT GTGTTTGCAT CACTATGAGA GTGCCTCGGA ACCCAACTAT CGGAGATAAA
TTTGCCAGTC GCCATGGGCA GAAGGGCATT TTAAGCAGAT TGTGGCCGGC TGAGGACATG
CCTTTTACTG AGAGTGGGAT GGTCCCAGAC ATTCTGTTCA ATCCCCATGG TTTTCCATCC
CGCATGACCA TTGGGATGTT AATTGAGAGT ATGGCCGGGA AGTCTGCAGC TTTGCATGGT
CTCTGCCATG ATGCTACACC CTTCATCTTC TCAGAGGAGA ACTCGGCCTT AGAATACTTT
GGTGAGATGT TAAAGGCTGC TGGCTACAAT TTCTATGGCA CCGAGAGGTT ATATAGTGGC
ATCAGTGGGC TAGAACTGGA AGCAGACATC TTCATAGGAG TGGTTTATTA TCAGCGCTTA
CGCCATATGG TCTCAGACAA ATTTCAAGTA AGGACAACTG GAGCCCGAGA CAGAGTCACC
AACCAGCCTA TTGGGGGAAG AAATGTCCAG GGTGGAATCC GTTTTGGGGA GATGGAACGG
GATGCGCTTT TAGCTCATGG TACATCTTTT CTCCTTCATG ACCGCCTCTT CAACTGCTCA
GATCGGTCGG TAGCCCATGT GTGTGTGAAG TGTGGCAGTT TACTCTCTCC ACTGTTGGAG
AAGCCACCCC CTTCTTGGTC TGCCATGCGC AACAGAAAAT ACAACTGTAC TCTGTGTAGT
CGCAGTGACA CTATCGATAC TGTTTCTGTG CCTTATGTTT TTCGGTATTT TGTAGCTGAA
CTGGCAGCTA TGAACATCAA AGTGAAACTG GATGTTGTTT AAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001269701.1 CDS 117..3638 Translation
Target ORF information:
RefSeq Version NM_001282772.2 Organism Homo sapiens(human) Definition Homo sapiens RNA polymerase I subunit B (POLR1B), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001282772.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481ATGAGAGCCA AAGAGGCTGC GGAAACGGGA CTGCGGCCAC TACTTCCGGC GTGTACCGAG
AGACTGGCGT CCGGCGTGTA CCGAGAGACT GGCGTCCGGT GTGCAGGTGG CCACATGGAT
CCTGGCAGCC GGTGGCGGAA CCTGCCCAGC GGGCCTAGCC TAAAGCACTT GACTGACCCC
TCTTATGGAA TCCCGCGGGA ACAGCAAAAG GCAGCGTTGC AGGAGCTGAC GCGGGCGCAC
GTGGAGTCCT TCAACTACGC TGTGCACGAG GGTCTCGGCC TCGCGGTGCA GGCTATACCT
CCCTTTGAAT TTGCTTTCAA AGATGAGCGT ATCTCTTTTA CTATTCTGGA TGCTGTTATC
AGTCCACCTA CAGTTCCAAA AGGGACCATC TGCAAAGAGG CCAATGTTTA TCCAGCAGAA
TGCCGGGGCC GAAGGAGTAC CTACCGTGGG AAGTTGACAG CTGATATCAA CTGGGCAGTG
AATGGAATCT CAAAAGGAAT CATTAAGCAG TTTCTTGGCT ATGTTCCCAT CATGGTGAAA
TCCAAGCTTT GCAACTTACG TAACCTTCCC CCACAAGCCC TCATTGAGCA CCATGAGGAG
GCAGAGGAAA TGGGGGGCTA TTTTATAATC AATGGCATTG AAAAAGTCAT CCGAATGTTG
ATTATGCCTC GGAGAAATTT TCCCATTGCA ATGATAAGAC CAAAATGGAA AACCAGAGGG
CCTGGTTATA CTCAGTATGG AGTTTCAATG CACTGTGTGA GGGAAGAACA TTCCGCTGTC
AATATGAACC TCCACTACTT GGAAAATGGC ACTGTTATGT TGAACTTTAT TTACCGAAAA
GAACTGTTCT TTCTTCCTTT GGGATTTGCA CTTAAGGCAC TTGTCAGCTT TTCTGATTAT
CAGATCTTTC AGGAGCTCAT CAAAGGAAAA GAGGATGATT CTTTCCTTAG GAACTCTGTT
TCTCAGATGT TAAGGATTGT AATGGAAGAG GGTTGTTCGA CACAAAAACA GGTCCTTAAC
TACCTAGGTG AATGCTTCAG AGTAAAACTC AATGTTCCTG ACTGGTACCC AAATGAGCAA
GCTGCGGAGT TCCTGTTTAA CCAGTGCATC TGTATCCACT TGAAATCCAA TACTGAAAAG
TTTTATATGC TTTGTCTCAT GACGCGAAAG CTCTTTGCTT TAGCCAAAGG AGAGTGCATG
GAGGACAATC CTGATAGTTT GGTGAACCAG GAAGTCCTCA CACCGGGTCA GCTCTTCCTT
ATGTTCCTGA AGGAAAAACT GGAAGGTTGG TTAGTGTCTA TTAAAATAGC TTTTGATAAG
AAGGCTCAGA AGACCAGTGT TTCCATGAAC ACTGACAATT TGATGAGGAT TTTTACAATG
GGCATAGACC TTACAAAACC ATTTGAATAC CTTTTTGCTA CTGGGAATCT GCGTTCTAAA
ACAGGTCTTG GCCTCCTACA AGATTCTGGA CTTTGTGTTG TGGCTGACAA GCTGAACTTC
ATACGCTACC TCTCCCATTT CCGCTGCGTG CACAGAGGGG CTGATTTTGC CAAGATGAGG
ACCACCACAG TACGCAGGCT GCTGCCAGAG TCCTGGGGCT TCCTTTGTCC CGTGCATACC
CCAGACGGGG AGCCCTGTGG CCTGATGAAC CACCTAACTG CCGTATGTGA GGTTGTCACA
CAGTTTGTGT ATACGGCATC TATTCCAGCT TTACTGTGCA ACTTGGGGGT CACTCCCATT
GATGGAGCTC CCCACCGATC ATACAGTGAG TGCTACCCTG TCCTGCTGGA CGGTGTCATG
GTTGGCTGGG TGGATAAGGA TCTTGCTCCA GGCATCGCAG ATTCTCTTCG TCATTTTAAG
GTGTTGAGAG AGAAAAGAAT TCCTCCCTGG ATGGAAGTGG TCCTTATACC CATGACAGGA
AAACCAAGTC TGTACCCAGG ATTGTTCCTT TTTACCACTC CTTGTAGACT GGTACGGCCT
GTGCAGAACT TAGCATTGGG CAAAGAAGAG CTAATTGGAA CTATGGAACA GATCTTCATG
AATGTCGCTA TCTTTGAGGA TGAAGTTTTT GCTGGAGTTA CCACACACCA GGAACTCTTT
CCACACAGCC TGCTGAGTGT GATTGCCAAC TTCATCCCTT TCTCTGATCA CAACCAGAGT
CCACGGAACA TGTACCAATG CCAGATGGGT AAGCAAACTA TGGGCTTTCC ACTTCTCACT
TATCAAGACC GATCGGATAA CAAACTGTAT CGTCTTCAGA CTCCTCAGAG TCCCTTGGTG
AGACCCTCCA TGTATGATTA TTATGACATG GATAACTATC CAATTGGGAC CAATGCCATC
GTTGCTGTGA TTTCTTACAC TGGCTATGAT ATGGAAGATG CCATGATTGT GAATAAGGCC
TCTTGGGAAC GAGGCTTTGC CCATGGAAGT GTCTACAAGT CTGAGTTCAT AGACCTCTCT
GAAAAAATTA AACAAGGAGA TAGTAGCCTG GTGTTTGGCA TCAAACCTGG TGACCCACGC
GTTCTGCAGA AGTTAGATGA CGATGGATTG CCGTTTATAG GAGCAAAACT GCAGTACGGA
GATCCGTATT ACAGCTACCT CAACCTCAAC ACCGGGGAAA GTTTTGTGAT GTACTATAAG
AGTAAAGAAA ATTGTGTTGT GGATAACATC AAAGTGTGCA GTAATGACAC TGGGAGTGGA
AAATTCAAGT GTGTTTGCAT CACTATGAGA GTGCCTCGGA ACCCAACTAT CGGAGATAAA
TTTGCCAGTC GCCATGGGCA GAAGGGCATT TTAAGCAGAT TGTGGCCGGC TGAGGACATG
CCTTTTACTG AGAGTGGGAT GGTCCCAGAC ATTCTGTTCA ATCCCCATGG TTTTCCATCC
CGCATGACCA TTGGGATGTT AATTGAGAGT ATGGCCGGGA AGTCTGCAGC TTTGCATGGT
CTCTGCCATG ATGCTACACC CTTCATCTTC TCAGAGGAGA ACTCGGCCTT AGAATACTTT
GGTGAGATGT TAAAGGCTGC TGGCTACAAT TTCTATGGCA CCGAGAGGTT ATATAGTGGC
ATCAGTGGGC TAGAACTGGA AGCAGACATC TTCATAGGAG TGGTTTATTA TCAGCGCTTA
CGCCATATGG TCTCAGACAA ATTTCAAGTA AGGACAACTG GAGCCCGAGA CAGAGTCACC
AACCAGCCTA TTGGGGGAAG AAATGTCCAG GGTGGAATCC GTTTTGGGGA GATGGAACGG
GATGCGCTTT TAGCTCATGG TACATCTTTT CTCCTTCATG ACCGCCTCTT CAACTGCTCA
GATCGGTCGG TAGCCCATGT GTGTGTGAAG TGTGGCAGTT TACTCTCTCC ACTGTTGGAG
AAGCCACCCC CTTCTTGGTC TGCCATGCGC AACAGAAAAT ACAACTGTAC TCTGTGTAGT
CGCAGTGACA CTATCGATAC TGTTTCTGTG CCTTATGTTT TTCGGTATTT TGTAGCTGAA
CTGGCAGCTA TGAACATCAA AGTGAAACTG GATGTTGTTT AAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
-
PubMed
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M
PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation.
Nature structural & molecular biology17(4)445-50(2010 Apr)
Feng W,Yonezawa M,Ye J,Jenuwein T,Grummt I
FACT facilitates chromatin transcription by RNA polymerases I and III.
The EMBO journal28(7)854-65(2009 Apr)
Birch JL,Tan BC,Panov KI,Panova TB,Andersen JS,Owen-Hughes TA,Russell J,Lee SC,Zomerdijk JC
Efficient protein expression from the endogenous RNA polymerase I promoter using a human ribosomal DNA targeting vector.
Biochemical and biophysical research communications367(4)846-51(2008 Mar)
Wen L,Liang DS,Wu LQ,Cai F,Pan Q,Long ZG,Dai HP,Xia K,Xia JH
Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases.
Molecular cell26(3)367-79(2007 May)
Johnson SS,Zhang C,Fromm J,Willis IM,Johnson DL
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