EHI_028930 cDNA ORF clone, Entamoeba histolytica HM-1:IMSS

The following EHI_028930 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHI_028930 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OEb00249 XM_645825.1
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Entamoeba histolytica HM-1:IMSS cyst wall-specific glycoprotein Jacob (EHI_028930), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OEb00249
    Clone ID Related Accession (Same CDS sequence) XM_645825.1
    Accession Version XM_645825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1508774400000
    Organism Entamoeba histolytica HM-1:IMSS
    Product cyst wall-specific glycoprotein Jacob
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001914981). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAGCAT TACTTGTTAT ATTATGTATG TATTTAGTTT CATCACTTGA ATTGGTAGAT 
    GGTGAATGTA AAGGAAGACA TGGATATTTC TGCTACACAG ACAATGATAA GAAGAAGAAT
    TATTATCTTT GTAACAAAGG AGTTCAAATG GGATGTGTTA GAACTTGTCC AAATGAAGAT
    GTTTGTACTG GTAGAGGTGA GTTTGAAATA ACAAAAGACA AGATTTGTGT TGCACTTAAT
    GAGTCACACT GGGAGGTTGA CCCATTACCA ACTCCTACAC CAAATAAATA TCCAGTCAAT
    TGTACTGAAG TGAAAGAAGG ATATTATTGC TATGAAGAAG AGTCAAAAAA TACTGAATAT
    TATTGGTGTG TTAACAATGT TGGCTATGAA ATGAAATGTC CTAATGGAAC AACATGTCAT
    ACAAAAGACA TTGGTTATAA CAATCCATGT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_650917.1
    CDS1..456
    Translation

    Target ORF information:

    RefSeq Version XM_645825.1
    Organism Entamoeba histolytica HM-1:IMSS
    Definition Entamoeba histolytica HM-1:IMSS cyst wall-specific glycoprotein Jacob (EHI_028930), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_645825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAGCAT TACTTGTTAT ATTATGTATG TATTTAGTTT CATCACTTGA ATTGGTAGAT 
    GGTGAATGTA AAGGAAGACA TGGATATTTC TGCTACACAG ACAATGATAA GAAGAAGAAT
    TATTATCTTT GTAACAAAGG AGTTCAAATG GGATGTGTTA GAACTTGTCC AAATGAAGAT
    GTTTGTACTG GTAGAGGTGA GTTTGAAATA ACAAAAGACA AGATTTGTGT TGCACTTAAT
    GAGTCACACT GGGAGGTTGA CCCATTACCA ACTCCTACAC CAAATAAATA TCCAGTCAAT
    TGTACTGAAG TGAAAGAAGG ATATTATTGC TATGAAGAAG AGTCAAAAAA TACTGAATAT
    TATTGGTGTG TTAACAATGT TGGCTATGAA ATGAAATGTC CTAATGGAAC AACATGTCAT
    ACAAAAGACA TTGGTTATAA CAATCCATGT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the protist parasite Entamoeba histolytica.
    Nature433(7028)865-8(2005 Feb)
    Loftus B,Anderson I,Davies R,Alsmark UC,Samuelson J,Amedeo P,Roncaglia P,Berriman M,Hirt RP,Mann BJ,Nozaki T,Suh B,Pop M,Duchene M,Ackers J,Tannich E,Leippe M,Hofer M,Bruchhaus I,Willhoeft U,Bhattacharya A,Chillingworth T,Churcher C,Hance Z,Harris B,Harris D,Jagels K,Moule S,Mungall K,Ormond D,Squares R,Whitehead S,Quail MA,Rabbinowitsch E,Norbertczak H,Price C,Wang Z,Guillén N,Gilchrist C,Stroup SE,Bhattacharya S,Lohia A,Foster PG,Sicheritz-Ponten T,Weber C,Singh U,Mukherjee C,El-Sayed NM,Petri WA Jr,Clark CG,Embley TM,Barrell B,Fraser CM,Hall N