Dpse\GA27747 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA27747
Entrez Gene ID	6903314
Full Name	GA27747 gene product from transcript GA27747-RA
Synonyms	GA27747,dpse_GLEANR_6864
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002132466.2	XP_002132502.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA27747 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA27747 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe14690	XM_002132466.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27747), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA27747 ( XM_002132466.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002132502.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27747), mRNA.

Price & Availability↑

CloneID	ODe14690
Clone ID Related Accession (Same CDS sequence)	XM_002132466.2
Accession Version	XM_002132466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589965). On Dec 22, 2015 this sequence version replaced XM_002132466.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACATCAA GTGGAGAAAA ACTGTCAAGC AATAATTGTA GAACTGGAAA AAAGTGTCCA 
GACAATGTTC ATTTCATAAT GCGTGCAAAC GGTAATATTA GCATCAGTCA AAGAAAATCT 
GTTAATTCCC AGAATCAATT TTCGTTGACC TTTGAATCAA ATGAATGTGA TGTGATGTAC 
TGCAGTTTGC AATCTTCTCG CTTAAATGCG CAGTCTAGTG ATGCTTTCCA GCCCAACGCT 
GGAAAGCTGA AGCTGTCGCC GCTGGAAAAG ATTGACGATG CCAATACCGC AGCCCCTCCA 
TCGCCCGCAC CTAATAATGA TGGTTTTGAG GCATGCGTTA ACCAGTTAAA AACTCATACA 
TGTAACGTAA CAATATCAAA GTCAGAAAAC CATGCCTTAG TTCCGAAGCA TGGTATCGGA 
ACAACAAGCT ACCATCAACG TGGCATATCC CCAAACTCAA GATATCGTCT GGAGCGGTAT 
AAAGAGGCTA AGCCAGCTCA ACAACCAAAA TTATTAGAAG CAGTAAATGT TCCATCAACA 
GTTCCATCTG TTTCCTCAAC TCGATTACTG CTACCATCCA ATGTAGCGCT ACCGATGGGT 
CAATGTGAGA ATTATAATGC TTATCTAGGA TCGGCGGTAC ATACACCAGT AAAGCGATAT 
GTGCCAACTC CACCGCCTGC TATTGATATG TATGCAGGCC TAACATTACA GGCCTCGTCA 
GCGAATATGA CCCCCCCGGC ATCATCAGTG CAGCCTCAGT ACGGCAATAT GCCCTATAAT 
TATCGTTCAA AATGCTGCCA TAATGGCAAT GTGGAATCTA TGCATGGAAG TTTGACCAAA 
CCATCACAAA CATATGTCGC GAGTGTATCC TGTTCAACAT CTTGTACCTC GCCATTGCCC 
GCACCATCAA CAAGTATTTC CATGGCTTTT TCAGCAGGCA ACCATTGCTC GCCAATTATG 
ACAGCTACTG CAGAGCAAAC GATAGCTCAC AGTAGCGACA ACTCACATAT AACCAGCCGA 
AGGTCCGAGG AAAGTGATTC TTTAATTGTT ATAGCACCCG GTACCACCAT AGCCCAATTA 
GAAGCGGACA GCCCCGCCGG CACGTGCATG CATTGCAATA CAGCACGACG AACCACTGGG 
GTACATCAGA CAACACAAAC GACTGGACCT ATTAGTCCTG TGCCGATATC TTTGTCTGTT 
CCTATGACTG CGCCAGTAAT ATCGAGTTTG AGTGAGCAAC TTGCAAATAC AAATACCGAA 
TCAAGTATCG TGTCCGTTCA AACGAAGCTC CATGATAGTA GAAATATTGC ACTGAAGCAG 
GGGTCGAGTC ATCATCAGTT GTACGGACCA GGTACTGCAG CTGCGTTTAG CCATCTTATA 
AGCAATCCAC GCTTACAGCA TACTCACCAC ACACAGACAC ATCAACACCA GTCGTTGCAA 
ATACTACCGC AGCCCCAAAT TCATCATTAC TCTTGCAAAA AGCGAATAGT AATCTATTTA 
AAACGTACAA CTTCCCAGTT TTTTGGAGTT GAGCCGAGTA CTGAGGCCGA AGATTGTGCT 
CTGTGGCATG GTCGACATCG TCGACTAGCT ATTCGTCGAT TTGGATTGTT CGATAGCGCA 
CTAGAATATC GTGTCCAACA GTCGATTAGA CACGGTGTCA ATGTTGAAGT TGGAAAGCAA 
AATAGTCCGG AAATGAAGCA GAACTGTTCT AACGCAAGCA GTAATTACGC ACAGGATCGC 
CCCGACATTC TACCAGTTCA AGATGCAATT GCTATGAATA TGACATATTC TTCAGATAAT 
AGGCGACCCA AATTCTACAC TAACAGAGAT TTTTGTGACG GTGAATTTGT AGAGCGTAAG 
GCGTCAGTTG GTCACATGTT TATAACAATG GTTAATTATT TGCTCCATTT TATTTATAAA 
GGGCAGACTA AGTGCAGCCA CAGAGTAAAG CATCAGTGGC AATGGTCACG TAGTTTTGCA 
CCGTTACATG TGCAAAGCAG CTCCAGTTTT GATCCGACTC ACAACGATCA ACCGATTGAT 
ATAGAAATAA GCAATAGCAT GACGGATGCT TTAGAAACGA TAATTGTTGA TGAACTCTTT 
TTTGATAGTC CTTGCGAAGT GTCTACTGCA TCTGCAAATG ATGACATATC TGATATTAGC 
AGACAAACCA TTAAGCCACG TGTCTCCACA CTGGGAACCA ATGGTACCAA TATGGGAGTT 
GGAGTAAGTG TGTACATGAC TGAAAGACAC CAAAACGGAT GGCGCACATC CGCTCTGATC 
AGCAATGCAT GCACAAATGG TGATATTAAT TTAAATGCTG ATCAAAATTT ACATCAAGTA 
AATGCGGGTC ATATACAATT GTGCAACTCA ATCCCTAGCC AAATACAACC ATCAATGCGA 
ACATCACAAT CTACAACATC TGCATGCCAA CGTGGTACAC GTATCACAGC TCAGCTGTTG 
GACGGAGTCT TGGAAAACTC AAGGCGAACC CCTTTACGGC GCATCAAATA TTTCTCAGTC 
AATGATTTAG ACGATCGAAC GGATCATCGA CCTATATTTA CCTATTGGAT TAATACCGTA 
CAGTTTTTAG TTTTAGTTCT ATCGATAATA TGTTATGGTA TTGCTCCAAT TGGAATTGGC 
TCTGAACAAA AAACAGGACA GGTACTGGTC ACCAGTCTAA GTCTGCAGAC GGTTCAACAT 
GTAGAACAAC GAAATCTGTG GATTGGGCCT AGGAATATTG ACTTAGTTCA CATTGGTGCA 
AAGTTTGCTG CCTGCATGCG ACGTGACAGT AAGATCATGG ACGTGGTTAT TAAGACAAGA 
CGTCACGAAA GGGAAACAGC CTGCTGTATC CGGAATGATG ACTCCGGATG CGTTCAAAGT 
TCTCAAGCGG ATTGTAGTAT AAGAGGCCTC TATCCTACAA AATCCATATC AACATGGAAA 
AAATGGTCGC CCGGCGAATC AGGACCTGGA GGTCGAATTT CTGGATCAGT TTGCGGCTTA 
GATCCAAAAT TTTGCGATGC ACCAGCGTCA ATAGCACCAT ATGAGTGGCC CGACGACATC 
ACAAAGTGGC CCATTTGTCG TAAAACGAAT TCATTCACGC AGCGCTATCG TTATAAAGAC 
CATACTTCTG AGCATATGGT CTGCGAAGTA ATTGGTCATC CTTGTTGCAC CGGGTTATAC 
GGAGAATGCC GAATAACGAC GCGGGAGTAT TGTGATTTTA TAAATGGATA TTTTCATGAG 
GAAGCCTCTC TCTGCTCACA GATATCATGC CTCAACAATG TTTGTGGAAT GTTACCATTT 
ATATCTGTGG AAACTCCAGA TCAGCTCTAT AGACTGCTAA CATCACTGTG CATGCATGCC 
GGTATCTTAC ACTTGGCAAT TACACTCATT TTCCAGCATT TGTTTCTTGC TGATTTGGAA 
AAATTAATTG GAACGCTACG AACTGCTGTT GTCTACATAG TTTCCGGCTT TGCAGGAAAT 
CTTACAAGCG CTATAATTGT ACCACATCGC CCAGAGGTTG GACCATCAGC ATCGCTCTGT 
GGTGTGGTGT CTTCGCTTAT TGCACTTTTG ATATGGATGC ATTGGAAATG TCTTCACAAG 
CCGCATATTG CTTTATTTAA ATTGTTACTC TTATGCAGTG TTCTGGTCGG AATCGGAACC 
CTACCCTATC AGCTGAATTT TGTTGGACTT TTGGCTGGAG TGGCTTGTGG TGGCCTTTTA 
ACTATGACCC TAGTGCCATT TACAACATTT TCGAAATACG GACGAAAAAA AAAGATAAAA 
CTTATTTGGA CATGTCTATT GTTCCATGTA GTAGTATACA GTGGTTTGAT TGCTACCTTT 
TACATTCATC CCAGTGAATT TCATACAATA AGCATTGTGG ATATTTTTAG TAATAGCAAC 
GGATACGATA ATGTCACCAA CTCTGACCAT CATGGTGTTG ACGTCATAAG CAGCACCACT 
AGATACACGG AGGCCCAAAA TCCCCAATTT TATTATCACC ATCATTCTGA TGATATTATC 
AGAAATAGTG TCGCATTCAC AGAAAGAGCT CTTGTTTCGC ACATAATATA TCCATCAACT 
CCACGGAAAG CAAGTGTTCA ACAGTGGCAG AAAGCTGAGT TGAGTCGTTC GTTGAATAAC 
TTTACAAATT ATAGTAATAT AAAAAAAAGT ATCGAACATA TATCAAACGT AAAAAAAATG 
TTTACCCTAC CTGTTAGATT AACAATTCAA AATAACCATT CAAATCTTAT GACTGAATTA 
ACAACAGTGA ATACCAAAAT GAACCAGAAA TATTTAGGTT ACATAAACAA CACAAAAGAA 
AAAAGCCCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002132502.1
CDS	345..4676
Translation	MTSSGEKLSSNNCRTGKKCPDNVHFIMRANGNISISQRKSVNSQNQFSLTFESNECDVMYCSLQSSRLNAQSSDAFQPNAGKLKLSPLEKIDDANTAAPPSPAPNNDGFEACVNQLKTHTCNVTISKSENHALVPKHGIGTTSYHQRGISPNSRYRLERYKEAKPAQQPKLLEAVNVPSTVPSVSSTRLLLPSNVALPMGQCENYNAYLGSAVHTPVKRYVPTPPPAIDMYAGLTLQASSANMTPPASSVQPQYGNMPYNYRSKCCHNGNVESMHGSLTKPSQTYVASVSCSTSCTSPLPAPSTSISMAFSAGNHCSPIMTATAEQTIAHSSDNSHITSRRSEESDSLIVIAPGTTIAQLEADSPAGTCMHCNTARRTTGVHQTTQTTGPISPVPISLSVPMTAPVISSLSEQLANTNTESSIVSVQTKLHDSRNIALKQGSSHHQLYGPGTAAAFSHLISNPRLQHTHHTQTHQHQSLQILPQPQIHHYSCKKRIVIYLKRTTSQFFGVEPSTEAEDCALWHGRHRRLAIRRFGLFDSALEYRVQQSIRHGVNVEVGKQNSPEMKQNCSNASSNYAQDRPDILPVQDAIAMNMTYSSDNRRPKFYTNRDFCDGEFVERKASVGHMFITMVNYLLHFIYKGQTKCSHRVKHQWQWSRSFAPLHVQSSSSFDPTHNDQPIDIEISNSMTDALETIIVDELFFDSPCEVSTASANDDISDISRQTIKPRVSTLGTNGTNMGVGVSVYMTERHQNGWRTSALISNACTNGDINLNADQNLHQVNAGHIQLCNSIPSQIQPSMRTSQSTTSACQRGTRITAQLLDGVLENSRRTPLRRIKYFSVNDLDDRTDHRPIFTYWINTVQFLVLVLSIICYGIAPIGIGSEQKTGQVLVTSLSLQTVQHVEQRNLWIGPRNIDLVHIGAKFAACMRRDSKIMDVVIKTRRHERETACCIRNDDSGCVQSSQADCSIRGLYPTKSISTWKKWSPGESGPGGRISGSVCGLDPKFCDAPASIAPYEWPDDITKWPICRKTNSFTQRYRYKDHTSEHMVCEVIGHPCCTGLYGECRITTREYCDFINGYFHEEASLCSQISCLNNVCGMLPFISVETPDQLYRLLTSLCMHAGILHLAITLIFQHLFLADLEKLIGTLRTAVVYIVSGFAGNLTSAIIVPHRPEVGPSASLCGVVSSLIALLIWMHWKCLHKPHIALFKLLLLCSVLVGIGTLPYQLNFVGLLAGVACGGLLTMTLVPFTTFSKYGRKKKIKLIWTCLLFHVVVYSGLIATFYIHPSEFHTISIVDIFSNSNGYDNVTNSDHHGVDVISSTTRYTEAQNPQFYYHHHSDDIIRNSVAFTERALVSHIIYPSTPRKASVQQWQKAELSRSLNNFTNYSNIKKSIEHISNVKKMFTLPVRLTIQNNHSNLMTELTTVNTKMNQKYLGYINNTKEKSP

Target ORF information:

RefSeq Version	XM_002132466.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27747), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002132466.2

ORF Insert Sequence:

ATGACATCAA GTGGAGAAAA ACTGTCAAGC AATAATTGTA GAACTGGAAA AAAGTGTCCA 
GACAATGTTC ATTTCATAAT GCGTGCAAAC GGTAATATTA GCATCAGTCA AAGAAAATCT 
GTTAATTCCC AGAATCAATT TTCGTTGACC TTTGAATCAA ATGAATGTGA TGTGATGTAC 
TGCAGTTTGC AATCTTCTCG CTTAAATGCG CAGTCTAGTG ATGCTTTCCA GCCCAACGCT 
GGAAAGCTGA AGCTGTCGCC GCTGGAAAAG ATTGACGATG CCAATACCGC AGCCCCTCCA 
TCGCCCGCAC CTAATAATGA TGGTTTTGAG GCATGCGTTA ACCAGTTAAA AACTCATACA 
TGTAACGTAA CAATATCAAA GTCAGAAAAC CATGCCTTAG TTCCGAAGCA TGGTATCGGA 
ACAACAAGCT ACCATCAACG TGGCATATCC CCAAACTCAA GATATCGTCT GGAGCGGTAT 
AAAGAGGCTA AGCCAGCTCA ACAACCAAAA TTATTAGAAG CAGTAAATGT TCCATCAACA 
GTTCCATCTG TTTCCTCAAC TCGATTACTG CTACCATCCA ATGTAGCGCT ACCGATGGGT 
CAATGTGAGA ATTATAATGC TTATCTAGGA TCGGCGGTAC ATACACCAGT AAAGCGATAT 
GTGCCAACTC CACCGCCTGC TATTGATATG TATGCAGGCC TAACATTACA GGCCTCGTCA 
GCGAATATGA CCCCCCCGGC ATCATCAGTG CAGCCTCAGT ACGGCAATAT GCCCTATAAT 
TATCGTTCAA AATGCTGCCA TAATGGCAAT GTGGAATCTA TGCATGGAAG TTTGACCAAA 
CCATCACAAA CATATGTCGC GAGTGTATCC TGTTCAACAT CTTGTACCTC GCCATTGCCC 
GCACCATCAA CAAGTATTTC CATGGCTTTT TCAGCAGGCA ACCATTGCTC GCCAATTATG 
ACAGCTACTG CAGAGCAAAC GATAGCTCAC AGTAGCGACA ACTCACATAT AACCAGCCGA 
AGGTCCGAGG AAAGTGATTC TTTAATTGTT ATAGCACCCG GTACCACCAT AGCCCAATTA 
GAAGCGGACA GCCCCGCCGG CACGTGCATG CATTGCAATA CAGCACGACG AACCACTGGG 
GTACATCAGA CAACACAAAC GACTGGACCT ATTAGTCCTG TGCCGATATC TTTGTCTGTT 
CCTATGACTG CGCCAGTAAT ATCGAGTTTG AGTGAGCAAC TTGCAAATAC AAATACCGAA 
TCAAGTATCG TGTCCGTTCA AACGAAGCTC CATGATAGTA GAAATATTGC ACTGAAGCAG 
GGGTCGAGTC ATCATCAGTT GTACGGACCA GGTACTGCAG CTGCGTTTAG CCATCTTATA 
AGCAATCCAC GCTTACAGCA TACTCACCAC ACACAGACAC ATCAACACCA GTCGTTGCAA 
ATACTACCGC AGCCCCAAAT TCATCATTAC TCTTGCAAAA AGCGAATAGT AATCTATTTA 
AAACGTACAA CTTCCCAGTT TTTTGGAGTT GAGCCGAGTA CTGAGGCCGA AGATTGTGCT 
CTGTGGCATG GTCGACATCG TCGACTAGCT ATTCGTCGAT TTGGATTGTT CGATAGCGCA 
CTAGAATATC GTGTCCAACA GTCGATTAGA CACGGTGTCA ATGTTGAAGT TGGAAAGCAA 
AATAGTCCGG AAATGAAGCA GAACTGTTCT AACGCAAGCA GTAATTACGC ACAGGATCGC 
CCCGACATTC TACCAGTTCA AGATGCAATT GCTATGAATA TGACATATTC TTCAGATAAT 
AGGCGACCCA AATTCTACAC TAACAGAGAT TTTTGTGACG GTGAATTTGT AGAGCGTAAG 
GCGTCAGTTG GTCACATGTT TATAACAATG GTTAATTATT TGCTCCATTT TATTTATAAA 
GGGCAGACTA AGTGCAGCCA CAGAGTAAAG CATCAGTGGC AATGGTCACG TAGTTTTGCA 
CCGTTACATG TGCAAAGCAG CTCCAGTTTT GATCCGACTC ACAACGATCA ACCGATTGAT 
ATAGAAATAA GCAATAGCAT GACGGATGCT TTAGAAACGA TAATTGTTGA TGAACTCTTT 
TTTGATAGTC CTTGCGAAGT GTCTACTGCA TCTGCAAATG ATGACATATC TGATATTAGC 
AGACAAACCA TTAAGCCACG TGTCTCCACA CTGGGAACCA ATGGTACCAA TATGGGAGTT 
GGAGTAAGTG TGTACATGAC TGAAAGACAC CAAAACGGAT GGCGCACATC CGCTCTGATC 
AGCAATGCAT GCACAAATGG TGATATTAAT TTAAATGCTG ATCAAAATTT ACATCAAGTA 
AATGCGGGTC ATATACAATT GTGCAACTCA ATCCCTAGCC AAATACAACC ATCAATGCGA 
ACATCACAAT CTACAACATC TGCATGCCAA CGTGGTACAC GTATCACAGC TCAGCTGTTG 
GACGGAGTCT TGGAAAACTC AAGGCGAACC CCTTTACGGC GCATCAAATA TTTCTCAGTC 
AATGATTTAG ACGATCGAAC GGATCATCGA CCTATATTTA CCTATTGGAT TAATACCGTA 
CAGTTTTTAG TTTTAGTTCT ATCGATAATA TGTTATGGTA TTGCTCCAAT TGGAATTGGC 
TCTGAACAAA AAACAGGACA GGTACTGGTC ACCAGTCTAA GTCTGCAGAC GGTTCAACAT 
GTAGAACAAC GAAATCTGTG GATTGGGCCT AGGAATATTG ACTTAGTTCA CATTGGTGCA 
AAGTTTGCTG CCTGCATGCG ACGTGACAGT AAGATCATGG ACGTGGTTAT TAAGACAAGA 
CGTCACGAAA GGGAAACAGC CTGCTGTATC CGGAATGATG ACTCCGGATG CGTTCAAAGT 
TCTCAAGCGG ATTGTAGTAT AAGAGGCCTC TATCCTACAA AATCCATATC AACATGGAAA 
AAATGGTCGC CCGGCGAATC AGGACCTGGA GGTCGAATTT CTGGATCAGT TTGCGGCTTA 
GATCCAAAAT TTTGCGATGC ACCAGCGTCA ATAGCACCAT ATGAGTGGCC CGACGACATC 
ACAAAGTGGC CCATTTGTCG TAAAACGAAT TCATTCACGC AGCGCTATCG TTATAAAGAC 
CATACTTCTG AGCATATGGT CTGCGAAGTA ATTGGTCATC CTTGTTGCAC CGGGTTATAC 
GGAGAATGCC GAATAACGAC GCGGGAGTAT TGTGATTTTA TAAATGGATA TTTTCATGAG 
GAAGCCTCTC TCTGCTCACA GATATCATGC CTCAACAATG TTTGTGGAAT GTTACCATTT 
ATATCTGTGG AAACTCCAGA TCAGCTCTAT AGACTGCTAA CATCACTGTG CATGCATGCC 
GGTATCTTAC ACTTGGCAAT TACACTCATT TTCCAGCATT TGTTTCTTGC TGATTTGGAA 
AAATTAATTG GAACGCTACG AACTGCTGTT GTCTACATAG TTTCCGGCTT TGCAGGAAAT 
CTTACAAGCG CTATAATTGT ACCACATCGC CCAGAGGTTG GACCATCAGC ATCGCTCTGT 
GGTGTGGTGT CTTCGCTTAT TGCACTTTTG ATATGGATGC ATTGGAAATG TCTTCACAAG 
CCGCATATTG CTTTATTTAA ATTGTTACTC TTATGCAGTG TTCTGGTCGG AATCGGAACC 
CTACCCTATC AGCTGAATTT TGTTGGACTT TTGGCTGGAG TGGCTTGTGG TGGCCTTTTA 
ACTATGACCC TAGTGCCATT TACAACATTT TCGAAATACG GACGAAAAAA AAAGATAAAA 
CTTATTTGGA CATGTCTATT GTTCCATGTA GTAGTATACA GTGGTTTGAT TGCTACCTTT 
TACATTCATC CCAGTGAATT TCATACAATA AGCATTGTGG ATATTTTTAG TAATAGCAAC 
GGATACGATA ATGTCACCAA CTCTGACCAT CATGGTGTTG ACGTCATAAG CAGCACCACT 
AGATACACGG AGGCCCAAAA TCCCCAATTT TATTATCACC ATCATTCTGA TGATATTATC 
AGAAATAGTG TCGCATTCAC AGAAAGAGCT CTTGTTTCGC ACATAATATA TCCATCAACT 
CCACGGAAAG CAAGTGTTCA ACAGTGGCAG AAAGCTGAGT TGAGTCGTTC GTTGAATAAC 
TTTACAAATT ATAGTAATAT AAAAAAAAGT ATCGAACATA TATCAAACGT AAAAAAAATG 
TTTACCCTAC CTGTTAGATT AACAATTCAA AATAACCATT CAAATCTTAT GACTGAATTA 
ACAACAGTGA ATACCAAAAT GAACCAGAAA TATTTAGGTT ACATAAACAA CACAAAAGAA 
AAAAGCCCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA27747 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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