Dpse\GA24563 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA24563
Entrez Gene ID	6900950
Full Name	GA24563 gene product from transcript GA24563-RA
Synonyms	GA24563,dpse_GLEANR_1495
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002134615.2	XP_002134651.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA24563 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA24563 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe12978	XM_002134615.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA24563), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA24563 ( XM_002134615.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002134651.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA24563), mRNA.

Price & Availability↑

CloneID	ODe12978
Clone ID Related Accession (Same CDS sequence)	XM_002134615.2
Accession Version	XM_002134615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589957). On Dec 22, 2015 this sequence version replaced XM_002134615.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACAGC ACGCTGACGT GGATGGTGCC CACCCGGAGG AGGAGGACGG TTCGGGCAGG 
GGAAAGGCAA AGGGAAAAGG GAGGGCCAAA AAGAAGGCCA AAAAGGATGC CCATCCGATG 
GTCGGCTACA CGCAACTCTT TCGCTACATC ACGGCCTGGG ACTATGTCCT GTTCGTGTCC 
GCCCTGATAG CAGCCCTCCT GCAGTCTCTG GTCTACCCGA TTGCCATTGT GGTGTACAGC 
GAACTGGTGG CCATGTTCAT CGACCGGACC CTGGGGCAGG GCACCAGCTC CATTACCATC 
GGGCTGTCGC TCTTCGGAGG CGGAAAGGTG CTGACCAACG CCACGTACGA GGAGAACATG 
GAGGAGCTGC GCAAGGACAG CGTCTCCTTT GGCATCCTCA TGACCTTGAA CACCCTCCTG 
ATGCTCTTCT CCGGCATGTA CTACGTGGAC GCCTTCAACC GCCTCGCCCT GCGGCTCACG 
GTGCGCATGC GGAGGGAGTT CTTCAAGGCC ACCCTCAGGC AGGAGATCGG GTGGCACGAC 
ATGGCCAAGG ACCAGAACTT TGCCGTCCGC ATAACAGACA ACATGGAGAA GATACGCAGC 
GGCATCGCCG AGAACCTGGG CCACTACGTG GAGATCCTGT GCGAGGTGCT CATCAGCGTG 
GTCCTGTCCT TCGTCTACGG CTGGAAGCTG GCCCTGTCGA TAGTCTTCTA CATACCCCTC 
ACCCTCGCGG TCAATTCCGC GGTGGCACAC TACCAGGGGA AACTCACGGC GAAGGAGCAG 
AGCTCGTATG TGCGCGCCAG CTCTGTGGTG GAGGAGGTGA TTGGCGCCAT TCGCACGGTG 
GTGGCCTTTG GGGGCGAGAA AAGCGAGTCC GTGCGCTACG ACACCCTCCT GAAGCCCGCC 
CTGAAGGCGG GCAAGTGGAA GGGCGCCTTC TCGGGGCTCA GCGACACGGT GATGAAGGCC 
ATGATGTTCA TCACGGGGGC GGGGGCCTTC TGGTACGGCG CCAATCTGAT TCTCTTCTAC 
AGGGACCCCG CGATCCCCAT CGAGGAGCGG GTGTACACCC CAGCGGTGGT CATGATCGTC 
ATTTCGGGGA TCATCGTGGC CGCCAATCAG ATCTCCCGCA CCTCCCCCTT CCTCGAGACG 
TTCGCGATGG CTCGGGGATC GGCGGCGGCC ATCTTCGAGG TCATCGATCG GCGATCCCTG 
ATTGACCCGC TCTCCAAGGC GGGCAAGATC CTCAACTACG GGCTGAAGGG GGCCGTGGAG 
TTTCGGGACG TGTTCTTTCG GTACCCTGCC AGGGAGGACG TCATCGTCCT TCGGGGACTC 
AATGTCGTCG TGGAGGAGGG ACAGACGGTG GCCCTCGTTG GACCCTCGGG CTGTGGCAAG 
TCCACCTGCA TCCAGCTTCT GCAGCGCTTC TACGACCCCA TCTTCGGGCA GGTCCTTCTC 
GATGGCGAGG ACGTCCGCAA GTACAACATC CAGTGGCTGC GCTCCAACAT CGCGGTGGTG 
GGCCAGGAGC CGGTGCTCTT TCAGGGCTCC ATCGGGGAGA ACATCCGCCA CGGGAAGCCG 
GAGGCCACCC AAAAGGAGGT CGAGGATGCC GCCAAGGCCG CCAATGCCCA CGACTTCATT 
GTGGCCCTCC ACAAGGGCTA CGACACAAAC ATCAGCGAAA AGGGAGTCCA GCTGTCGGGC 
GGACAGCGAC AGCGCATTGC CATCGCCCGG GCCCTCATCC AGCAGCCCAA GATCCTGCTC 
CTGGACGAGG CCACCTCCGC GCTGGACTAC CACTCGGAGA AGCTCGTCCA GGAGGCCCTC 
GACAAGGCCT GCAGGGGGCG CACCACGCTG GTGGTCTCCC ACCGGCTCTC CGCCATCCGC 
CACGCCCATC GGATCGTGTA CATCGAGCAG GGGAAGGCGG TGGAGCAGGG CACCCACGAG 
GAGCTAATGA AAATCGAAGG ATTCTACCAC AAAATGGTCA CCGTTCACGC GTACGACGAC 
TCGGCCGAGG AGCTAATGAA CGAAATGGAG GAGGAGGCCG CCGTCCCGAA GAAGGAGCGC 
AAGAGCTCCG CGTACGATGC GGAGCCGCAG GCGCTGGAGA AGAACGCCTT CCAGATGAAG 
CACCTGAACG GCGTGGCGCC GCCCAGCACG CCGCAGGAGG ACGTGGACCC CCAGGAGCCT 
GCGACGGGCG GCAACTACAT TCGCACCTTC TTCCGGATCG TCGTGGCCGC TCGGCCCGAG 
TGGAGTTTCC TCATCATTGG AGCCATCTGC GCGGGCATCT ACGGCGTCAC GATGCCCGTC 
TTCTCCATCG TCCTGGCGGA GCTCTACGGC TCGCTGGCGA AGCCCACCGA CGAGGAGGTG 
CTCGATCAGA GCTCCTCGAT GGCCATCATT TCGCTGGTGA TTGGCGTCGC CGCCGGCATC 
GTGTGCTTCA TTCAAACGTT TTTCTTCAAT CTGGCGGGTG TGTGGCTCAC GATGCGAATG 
CGCTCGAGGA CCTTCAGCAG CATCATGCAG CAGGAGATGG GCTGGTTCGA CCGCAAGGAC 
AACAGCATTG GGGCCCTCTC GGCCCGGCTT TCGGGCGACG CAGCCAGTGT CCAGGGCGCC 
ATCGGCTTCC CGCTGAGCAA CATCATCCAG GCGCTGACGA ACTTCATCTG CAGCATTGCC 
ATCGCCTTTC CGTACTCCTG GGAGCTGGCC CTGATCTGCC TGAGCACGGC CCCCTTCATG 
ATTGCCTCGA TCGTCTTCGA GGCTCGCTTC GGGGAGCGGT CGGCGCTGAA GGAGAAGGAT 
GTCCTGGAGG AGACGAGTCG CATAGCCACG GAGACCATCG CCCAGATCCG CACTGTCGCC 
GGCCTGCGCC GCGAGGCGGA GCTGATTCGG GTCTACGACC AGGAGGTGGA GCGCTACCGC 
GTGCAGATCC TCACCCGCCT CAAGTGGCGG GGACTCGTCA ATTCCTTGGG CAAGTCCCTC 
ATGTTCTTCG GCTACGCGGT GACCCTGACC TACGGCGGAC ACATGTGCGC CGACGGGAAG 
ATCAAATTCG AGACGATCAT GAAGATCTCC AATACCATGC TCTACGGACT GTTCATCCTC 
GCCCAGTCGC TGGCCTTCAC GCCGGCCTTC AATGCGGCCC TCCTCTCCGC CAACCGCATG 
TACGAGATCA TCGACCGGCG GCCGCAGATC CAGTCGCCGG ATCCGGGGAT CGCGTCCCTG 
CTGCAGAACG GCAGTGGATC GCCGCACAAG ACGAATGTGG GGGTGCAGCA GGGCGTCTGC 
TACCGGGGCC TCCAGTTCGC GTATCCGTCG AGGCCCCACC TCAAGGTCCT GCAGGACTTC 
AACCTGGAGA TACAGCAGGG ACAAACGGTG GCCCTCGTGG GCGCCTCCGG GTCGGGCAAG 
TCCACCTGCG TGCAGCTCCT TTTGCGCTAC TACGATCCCG ACGAGGGGAA GATTCTGATC 
GATCAGGAGA GCATCCACCA GGATATGGGG CTCAAGACCC TGCGCCGCCG CCTGGGTCTC 
GTCTCGCAGG AGCCCTCGCT CTTCGAGAAA TCCATCGCCG AGAACATTGG CTACGGGGAC 
ACCAGCCGCA CGATTCCCAT GCAGCAGATC ATCGACGCCG CCAAGATGGC CAATGCCCAC 
GAGTTCATCA TGTCCCTGCC CGCCCAGTAC GACACCGTCC TGGGGTCAAA GGGCACCCAG 
CTGTCCGGTG GCCAGAAGCA GCGCATCGCC ATTGCCCGGG CGATGGTGAG GAACCCCAAG 
ATCCTGCTCC TGGACGAGGC CACCTCCGCC CTGGACTTCC AGAGTGAAAG GGTGGTGCAG 
CAGGCCCTCG ACTCCGCGTG CAGCGGACGC ACCAGCATCG TGATTGCCCA TCGCCTGTCC 
ACCATTCAGA ACGCGAGCGT CATTTGCGTG ATTCAAGCGG GACGCATCGC GGAGCAGGGC 
TCCCACGCGC AGCTGCTGGC CAAGAACGGC ATCTACTCGA AGCTGTATCG CAGCCAGACG 
AAGGCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002134651.1
CDS	535..4506
Translation	MPQHADVDGAHPEEEDGSGRGKAKGKGRAKKKAKKDAHPMVGYTQLFRYITAWDYVLFVSALIAALLQSLVYPIAIVVYSELVAMFIDRTLGQGTSSITIGLSLFGGGKVLTNATYEENMEELRKDSVSFGILMTLNTLLMLFSGMYYVDAFNRLALRLTVRMRREFFKATLRQEIGWHDMAKDQNFAVRITDNMEKIRSGIAENLGHYVEILCEVLISVVLSFVYGWKLALSIVFYIPLTLAVNSAVAHYQGKLTAKEQSSYVRASSVVEEVIGAIRTVVAFGGEKSESVRYDTLLKPALKAGKWKGAFSGLSDTVMKAMMFITGAGAFWYGANLILFYRDPAIPIEERVYTPAVVMIVISGIIVAANQISRTSPFLETFAMARGSAAAIFEVIDRRSLIDPLSKAGKILNYGLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPIFGQVLLDGEDVRKYNIQWLRSNIAVVGQEPVLFQGSIGENIRHGKPEATQKEVEDAAKAANAHDFIVALHKGYDTNISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQEALDKACRGRTTLVVSHRLSAIRHAHRIVYIEQGKAVEQGTHEELMKIEGFYHKMVTVHAYDDSAEELMNEMEEEAAVPKKERKSSAYDAEPQALEKNAFQMKHLNGVAPPSTPQEDVDPQEPATGGNYIRTFFRIVVAARPEWSFLIIGAICAGIYGVTMPVFSIVLAELYGSLAKPTDEEVLDQSSSMAIISLVIGVAAGIVCFIQTFFFNLAGVWLTMRMRSRTFSSIMQQEMGWFDRKDNSIGALSARLSGDAASVQGAIGFPLSNIIQALTNFICSIAIAFPYSWELALICLSTAPFMIASIVFEARFGERSALKEKDVLEETSRIATETIAQIRTVAGLRREAELIRVYDQEVERYRVQILTRLKWRGLVNSLGKSLMFFGYAVTLTYGGHMCADGKIKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRRPQIQSPDPGIASLLQNGSGSPHKTNVGVQQGVCYRGLQFAYPSRPHLKVLQDFNLEIQQGQTVALVGASGSGKSTCVQLLLRYYDPDEGKILIDQESIHQDMGLKTLRRRLGLVSQEPSLFEKSIAENIGYGDTSRTIPMQQIIDAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACSGRTSIVIAHRLSTIQNASVICVIQAGRIAEQGSHAQLLAKNGIYSKLYRSQTKAS

Target ORF information:

RefSeq Version	XM_002134615.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA24563), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002134615.2

ORF Insert Sequence:

ATGCCACAGC ACGCTGACGT GGATGGTGCC CACCCGGAGG AGGAGGACGG TTCGGGCAGG 
GGAAAGGCAA AGGGAAAAGG GAGGGCCAAA AAGAAGGCCA AAAAGGATGC CCATCCGATG 
GTCGGCTACA CGCAACTCTT TCGCTACATC ACGGCCTGGG ACTATGTCCT GTTCGTGTCC 
GCCCTGATAG CAGCCCTCCT GCAGTCTCTG GTCTACCCGA TTGCCATTGT GGTGTACAGC 
GAACTGGTGG CCATGTTCAT CGACCGGACC CTGGGGCAGG GCACCAGCTC CATTACCATC 
GGGCTGTCGC TCTTCGGAGG CGGAAAGGTG CTGACCAACG CCACGTACGA GGAGAACATG 
GAGGAGCTGC GCAAGGACAG CGTCTCCTTT GGCATCCTCA TGACCTTGAA CACCCTCCTG 
ATGCTCTTCT CCGGCATGTA CTACGTGGAC GCCTTCAACC GCCTCGCCCT GCGGCTCACG 
GTGCGCATGC GGAGGGAGTT CTTCAAGGCC ACCCTCAGGC AGGAGATCGG GTGGCACGAC 
ATGGCCAAGG ACCAGAACTT TGCCGTCCGC ATAACAGACA ACATGGAGAA GATACGCAGC 
GGCATCGCCG AGAACCTGGG CCACTACGTG GAGATCCTGT GCGAGGTGCT CATCAGCGTG 
GTCCTGTCCT TCGTCTACGG CTGGAAGCTG GCCCTGTCGA TAGTCTTCTA CATACCCCTC 
ACCCTCGCGG TCAATTCCGC GGTGGCACAC TACCAGGGGA AACTCACGGC GAAGGAGCAG 
AGCTCGTATG TGCGCGCCAG CTCTGTGGTG GAGGAGGTGA TTGGCGCCAT TCGCACGGTG 
GTGGCCTTTG GGGGCGAGAA AAGCGAGTCC GTGCGCTACG ACACCCTCCT GAAGCCCGCC 
CTGAAGGCGG GCAAGTGGAA GGGCGCCTTC TCGGGGCTCA GCGACACGGT GATGAAGGCC 
ATGATGTTCA TCACGGGGGC GGGGGCCTTC TGGTACGGCG CCAATCTGAT TCTCTTCTAC 
AGGGACCCCG CGATCCCCAT CGAGGAGCGG GTGTACACCC CAGCGGTGGT CATGATCGTC 
ATTTCGGGGA TCATCGTGGC CGCCAATCAG ATCTCCCGCA CCTCCCCCTT CCTCGAGACG 
TTCGCGATGG CTCGGGGATC GGCGGCGGCC ATCTTCGAGG TCATCGATCG GCGATCCCTG 
ATTGACCCGC TCTCCAAGGC GGGCAAGATC CTCAACTACG GGCTGAAGGG GGCCGTGGAG 
TTTCGGGACG TGTTCTTTCG GTACCCTGCC AGGGAGGACG TCATCGTCCT TCGGGGACTC 
AATGTCGTCG TGGAGGAGGG ACAGACGGTG GCCCTCGTTG GACCCTCGGG CTGTGGCAAG 
TCCACCTGCA TCCAGCTTCT GCAGCGCTTC TACGACCCCA TCTTCGGGCA GGTCCTTCTC 
GATGGCGAGG ACGTCCGCAA GTACAACATC CAGTGGCTGC GCTCCAACAT CGCGGTGGTG 
GGCCAGGAGC CGGTGCTCTT TCAGGGCTCC ATCGGGGAGA ACATCCGCCA CGGGAAGCCG 
GAGGCCACCC AAAAGGAGGT CGAGGATGCC GCCAAGGCCG CCAATGCCCA CGACTTCATT 
GTGGCCCTCC ACAAGGGCTA CGACACAAAC ATCAGCGAAA AGGGAGTCCA GCTGTCGGGC 
GGACAGCGAC AGCGCATTGC CATCGCCCGG GCCCTCATCC AGCAGCCCAA GATCCTGCTC 
CTGGACGAGG CCACCTCCGC GCTGGACTAC CACTCGGAGA AGCTCGTCCA GGAGGCCCTC 
GACAAGGCCT GCAGGGGGCG CACCACGCTG GTGGTCTCCC ACCGGCTCTC CGCCATCCGC 
CACGCCCATC GGATCGTGTA CATCGAGCAG GGGAAGGCGG TGGAGCAGGG CACCCACGAG 
GAGCTAATGA AAATCGAAGG ATTCTACCAC AAAATGGTCA CCGTTCACGC GTACGACGAC 
TCGGCCGAGG AGCTAATGAA CGAAATGGAG GAGGAGGCCG CCGTCCCGAA GAAGGAGCGC 
AAGAGCTCCG CGTACGATGC GGAGCCGCAG GCGCTGGAGA AGAACGCCTT CCAGATGAAG 
CACCTGAACG GCGTGGCGCC GCCCAGCACG CCGCAGGAGG ACGTGGACCC CCAGGAGCCT 
GCGACGGGCG GCAACTACAT TCGCACCTTC TTCCGGATCG TCGTGGCCGC TCGGCCCGAG 
TGGAGTTTCC TCATCATTGG AGCCATCTGC GCGGGCATCT ACGGCGTCAC GATGCCCGTC 
TTCTCCATCG TCCTGGCGGA GCTCTACGGC TCGCTGGCGA AGCCCACCGA CGAGGAGGTG 
CTCGATCAGA GCTCCTCGAT GGCCATCATT TCGCTGGTGA TTGGCGTCGC CGCCGGCATC 
GTGTGCTTCA TTCAAACGTT TTTCTTCAAT CTGGCGGGTG TGTGGCTCAC GATGCGAATG 
CGCTCGAGGA CCTTCAGCAG CATCATGCAG CAGGAGATGG GCTGGTTCGA CCGCAAGGAC 
AACAGCATTG GGGCCCTCTC GGCCCGGCTT TCGGGCGACG CAGCCAGTGT CCAGGGCGCC 
ATCGGCTTCC CGCTGAGCAA CATCATCCAG GCGCTGACGA ACTTCATCTG CAGCATTGCC 
ATCGCCTTTC CGTACTCCTG GGAGCTGGCC CTGATCTGCC TGAGCACGGC CCCCTTCATG 
ATTGCCTCGA TCGTCTTCGA GGCTCGCTTC GGGGAGCGGT CGGCGCTGAA GGAGAAGGAT 
GTCCTGGAGG AGACGAGTCG CATAGCCACG GAGACCATCG CCCAGATCCG CACTGTCGCC 
GGCCTGCGCC GCGAGGCGGA GCTGATTCGG GTCTACGACC AGGAGGTGGA GCGCTACCGC 
GTGCAGATCC TCACCCGCCT CAAGTGGCGG GGACTCGTCA ATTCCTTGGG CAAGTCCCTC 
ATGTTCTTCG GCTACGCGGT GACCCTGACC TACGGCGGAC ACATGTGCGC CGACGGGAAG 
ATCAAATTCG AGACGATCAT GAAGATCTCC AATACCATGC TCTACGGACT GTTCATCCTC 
GCCCAGTCGC TGGCCTTCAC GCCGGCCTTC AATGCGGCCC TCCTCTCCGC CAACCGCATG 
TACGAGATCA TCGACCGGCG GCCGCAGATC CAGTCGCCGG ATCCGGGGAT CGCGTCCCTG 
CTGCAGAACG GCAGTGGATC GCCGCACAAG ACGAATGTGG GGGTGCAGCA GGGCGTCTGC 
TACCGGGGCC TCCAGTTCGC GTATCCGTCG AGGCCCCACC TCAAGGTCCT GCAGGACTTC 
AACCTGGAGA TACAGCAGGG ACAAACGGTG GCCCTCGTGG GCGCCTCCGG GTCGGGCAAG 
TCCACCTGCG TGCAGCTCCT TTTGCGCTAC TACGATCCCG ACGAGGGGAA GATTCTGATC 
GATCAGGAGA GCATCCACCA GGATATGGGG CTCAAGACCC TGCGCCGCCG CCTGGGTCTC 
GTCTCGCAGG AGCCCTCGCT CTTCGAGAAA TCCATCGCCG AGAACATTGG CTACGGGGAC 
ACCAGCCGCA CGATTCCCAT GCAGCAGATC ATCGACGCCG CCAAGATGGC CAATGCCCAC 
GAGTTCATCA TGTCCCTGCC CGCCCAGTAC GACACCGTCC TGGGGTCAAA GGGCACCCAG 
CTGTCCGGTG GCCAGAAGCA GCGCATCGCC ATTGCCCGGG CGATGGTGAG GAACCCCAAG 
ATCCTGCTCC TGGACGAGGC CACCTCCGCC CTGGACTTCC AGAGTGAAAG GGTGGTGCAG 
CAGGCCCTCG ACTCCGCGTG CAGCGGACGC ACCAGCATCG TGATTGCCCA TCGCCTGTCC 
ACCATTCAGA ACGCGAGCGT CATTTGCGTG ATTCAAGCGG GACGCATCGC GGAGCAGGGC 
TCCCACGCGC AGCTGCTGGC CAAGAACGGC ATCTACTCGA AGCTGTATCG CAGCCAGACG 
AAGGCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA24563 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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