Dpse\GA16425 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA16425
Entrez Gene ID	4816446
Full Name	GA16425 gene product from transcript GA16425-RA
Synonyms	GA16425,dpse_GLEANR_9017
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356092.3	XP_001356128.2	uncharacterized protein, isoform A
XM_015181245.1	XP_015036731.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA16425 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA16425 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10680	XM_001356092.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA16425), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe18643	XM_015181245.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA16425), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA16425 ( XM_001356092.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356128.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA16425), mRNA.

Price & Availability↑

CloneID	ODe10680
Clone ID Related Accession (Same CDS sequence)	XM_001356092.3
Accession Version	XM_001356092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). On Dec 22, 2015 this sequence version replaced XM_001356092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCAT TTTTGCGGGT TTTATTACTT TGTGCGCATT TGTTGCATTT TAAATCAATC 
TCGGGAGTGG ATCTGGATCT GCCAGAGGTA TTTGGCGGGG AAGGAGCTGC AGGAGCGCCT 
GTGTGGCAGA ATGGTGAAAT CAAGGCGGCG CCCGAGGCAG GATCTGCCGT AGTAAATGCC 
ACAGTGGAGG AGAACATTGG TGTGTCCGTA GAAGATCCCT CGTCAGCGAG CCTGAGTCGG 
AGACAGCGTC AACTCTTCTT CGACCCGAAT CTCTTCCTCC TCCAAGGTGG CCTGGGCGGC 
CTAGGAGGTC TTGGCGGCCT TGGCGGTCTT AGCGGCTCTG GTGGATGGGA GGGATCCAAG 
TGTCTCACGG TCCAGGGTCA GGCCGGCGCT TGTGTCCGCA TCAACAACTG CCGCCAGTAC 
TACCGAGTGG CCCGCCAGCT GACCTTCATC GCCCTGCGGC AATGGCCCCA GAACCCTCCC 
CTTGGCCTGG GCGGAAATAT GTGCAACTTT TTCGACCAGC TGGGACGCAT CAACAACGGT 
ATCTGCTGCA CGGACATGGA TGCCAACACT TTCGGAGTAT TCCCGCTGCC GACCACAACG 
ACGACAGAGA GACCTTCGCC GGCTGCCGAT GAGGGCGAAA ATGGGGATGA GCCAGAGGAG 
GAGCCAGTCA ATGTTGACAC TGTGGTTCAA CCGGCGGAGA CACCACATCC AGAGGATCCC 
ATTGATAATG TGAACAGTGT AGAGGACACA CCGGACTTTC AGGATGTGAA CACAATCGAG 
GCTAATGCCC CAGAACCCGA ACCTGAGGCG GAACCAGAAC TAGAGACGAT ACCAGTGCCC 
GCCCAGCCGG CCACACCCGT CTACCCGTAT CAGTGGCCAT TCGGCTCCTT TGCCGGAGGC 
CCAGTAGCCC AATGGCCGCC ACCGCTGCCC ACCCATCCGC CTACAACTGG AGGCTGGCCC 
CCACCGCTTC CAACCCATCC GCCCAACCAC CACTATCCGA CGCATCCTTC GACGTCCGGA 
GTGCCCAGAC CCACCACAGT GCCCAGCACC AAGCGCACCA CCCCAAGACC CGCTGCCAGG 
CCGACTACCA CCAAGCGTCC CACTTATCCC AGCTATCCGG TGACCTCAGC GCCTGCGCCG 
ACGACCACGC GACGTCCGGT TAGCGGCTCC AGTCCCGAGG GCCTGCCGCT GCAGTGTGGC 
AACAAGAATC CGGTTTCGCC GGATCAGGAG CGCATTGTGG GAGGGATTAA CGCCAGTCCG 
CACGAGTTCC CCTGGATAGC GGTGCTATTC AAGTCGGGCA AGCAGTTCTG CGGCGGCAGT 
CTGATAACCA ACAATCACAT CCTGACAGCA GCACATTGTG TGGCACGCAT GACCTCGTGG 
GATGTGGCGG CCTTGACGGC CCACTTGGGC GACTACAATA TCCGGACGGA CTTTGAGGTG 
CAGCATGTCT CGCGCAGGAT CAAACGACTG GTGCGGCACA AAGGCTTCGA GTTCAGCACG 
CTGCACAACG ATGTGGCTAT TCTGACTCTC AGTGAGCCGG TGCCCTTCAC CCACGAGATC 
CAGCCGATTT GCCTGCCAAC GTCTCTGTCG CAGCAGTCGC GCTCGTATAG CGGCCAAGTG 
GCGACTGTGG CCGGCTGGGG CAGTCTGCGG GAGAACGGGC CCCAACCCTC GATTCTGCAG 
AAGGTGGACA TACCCATTTG GGCGAATGCC GAGTGTGCTC GCAAATACGG ACGTGCAGCG 
CCCGGTGGCA TCATCGAATC GATGATCTGT GCTGGCCAGG CCGCCAAGGA TTCCTGCAGC 
GGCGACTCTG GTGGTCCTAT GATCATCAAC GATGGCGGAC GATACACACA GGTGGGCATC 
GTTTCCTGGG GCATTGGCTG TGGCAAGGGT CAATATCCGG GCGTCTATAC GCGTGTCACG 
TCGCTGCTGC CCTGGATCTA CAAGAATATC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356128.2
CDS	1659..3614
Translation	MFAFLRVLLLCAHLLHFKSISGVDLDLPEVFGGEGAAGAPVWQNGEIKAAPEAGSAVVNATVEENIGVSVEDPSSASLSRRQRQLFFDPNLFLLQGGLGGLGGLGGLGGLSGSGGWEGSKCLTVQGQAGACVRINNCRQYYRVARQLTFIALRQWPQNPPLGLGGNMCNFFDQLGRINNGICCTDMDANTFGVFPLPTTTTTERPSPAADEGENGDEPEEEPVNVDTVVQPAETPHPEDPIDNVNSVEDTPDFQDVNTIEANAPEPEPEAEPELETIPVPAQPATPVYPYQWPFGSFAGGPVAQWPPPLPTHPPTTGGWPPPLPTHPPNHHYPTHPSTSGVPRPTTVPSTKRTTPRPAARPTTTKRPTYPSYPVTSAPAPTTTRRPVSGSSPEGLPLQCGNKNPVSPDQERIVGGINASPHEFPWIAVLFKSGKQFCGGSLITNNHILTAAHCVARMTSWDVAALTAHLGDYNIRTDFEVQHVSRRIKRLVRHKGFEFSTLHNDVAILTLSEPVPFTHEIQPICLPTSLSQQSRSYSGQVATVAGWGSLRENGPQPSILQKVDIPIWANAECARKYGRAAPGGIIESMICAGQAAKDSCSGDSGGPMIINDGGRYTQVGIVSWGIGCGKGQYPGVYTRVTSLLPWIYKNIK

Target ORF information:

RefSeq Version	XM_001356092.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA16425), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356092.3

ORF Insert Sequence:

ATGTTTGCAT TTTTGCGGGT TTTATTACTT TGTGCGCATT TGTTGCATTT TAAATCAATC 
TCGGGAGTGG ATCTGGATCT GCCAGAGGTA TTTGGCGGGG AAGGAGCTGC AGGAGCGCCT 
GTGTGGCAGA ATGGTGAAAT CAAGGCGGCG CCCGAGGCAG GATCTGCCGT AGTAAATGCC 
ACAGTGGAGG AGAACATTGG TGTGTCCGTA GAAGATCCCT CGTCAGCGAG CCTGAGTCGG 
AGACAGCGTC AACTCTTCTT CGACCCGAAT CTCTTCCTCC TCCAAGGTGG CCTGGGCGGC 
CTAGGAGGTC TTGGCGGCCT TGGCGGTCTT AGCGGCTCTG GTGGATGGGA GGGATCCAAG 
TGTCTCACGG TCCAGGGTCA GGCCGGCGCT TGTGTCCGCA TCAACAACTG CCGCCAGTAC 
TACCGAGTGG CCCGCCAGCT GACCTTCATC GCCCTGCGGC AATGGCCCCA GAACCCTCCC 
CTTGGCCTGG GCGGAAATAT GTGCAACTTT TTCGACCAGC TGGGACGCAT CAACAACGGT 
ATCTGCTGCA CGGACATGGA TGCCAACACT TTCGGAGTAT TCCCGCTGCC GACCACAACG 
ACGACAGAGA GACCTTCGCC GGCTGCCGAT GAGGGCGAAA ATGGGGATGA GCCAGAGGAG 
GAGCCAGTCA ATGTTGACAC TGTGGTTCAA CCGGCGGAGA CACCACATCC AGAGGATCCC 
ATTGATAATG TGAACAGTGT AGAGGACACA CCGGACTTTC AGGATGTGAA CACAATCGAG 
GCTAATGCCC CAGAACCCGA ACCTGAGGCG GAACCAGAAC TAGAGACGAT ACCAGTGCCC 
GCCCAGCCGG CCACACCCGT CTACCCGTAT CAGTGGCCAT TCGGCTCCTT TGCCGGAGGC 
CCAGTAGCCC AATGGCCGCC ACCGCTGCCC ACCCATCCGC CTACAACTGG AGGCTGGCCC 
CCACCGCTTC CAACCCATCC GCCCAACCAC CACTATCCGA CGCATCCTTC GACGTCCGGA 
GTGCCCAGAC CCACCACAGT GCCCAGCACC AAGCGCACCA CCCCAAGACC CGCTGCCAGG 
CCGACTACCA CCAAGCGTCC CACTTATCCC AGCTATCCGG TGACCTCAGC GCCTGCGCCG 
ACGACCACGC GACGTCCGGT TAGCGGCTCC AGTCCCGAGG GCCTGCCGCT GCAGTGTGGC 
AACAAGAATC CGGTTTCGCC GGATCAGGAG CGCATTGTGG GAGGGATTAA CGCCAGTCCG 
CACGAGTTCC CCTGGATAGC GGTGCTATTC AAGTCGGGCA AGCAGTTCTG CGGCGGCAGT 
CTGATAACCA ACAATCACAT CCTGACAGCA GCACATTGTG TGGCACGCAT GACCTCGTGG 
GATGTGGCGG CCTTGACGGC CCACTTGGGC GACTACAATA TCCGGACGGA CTTTGAGGTG 
CAGCATGTCT CGCGCAGGAT CAAACGACTG GTGCGGCACA AAGGCTTCGA GTTCAGCACG 
CTGCACAACG ATGTGGCTAT TCTGACTCTC AGTGAGCCGG TGCCCTTCAC CCACGAGATC 
CAGCCGATTT GCCTGCCAAC GTCTCTGTCG CAGCAGTCGC GCTCGTATAG CGGCCAAGTG 
GCGACTGTGG CCGGCTGGGG CAGTCTGCGG GAGAACGGGC CCCAACCCTC GATTCTGCAG 
AAGGTGGACA TACCCATTTG GGCGAATGCC GAGTGTGCTC GCAAATACGG ACGTGCAGCG 
CCCGGTGGCA TCATCGAATC GATGATCTGT GCTGGCCAGG CCGCCAAGGA TTCCTGCAGC 
GGCGACTCTG GTGGTCCTAT GATCATCAAC GATGGCGGAC GATACACACA GGTGGGCATC 
GTTTCCTGGG GCATTGGCTG TGGCAAGGGT CAATATCCGG GCGTCTATAC GCGTGTCACG 
TCGCTGCTGC CCTGGATCTA CAAGAATATC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA16425 ( XM_015181245.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036731.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA16425), mRNA.

Price & Availability↑

CloneID	ODe18643
Clone ID Related Accession (Same CDS sequence)	XM_015181245.1
Accession Version	XM_015181245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGATC CCTCGTCAGC GAGCCTGAGT CGGAGACAGC GTCAACTCTT CTTCGACCCG 
AATCTCTTCC TCCTCCAAGG TGGCCTGGGC GGCCTAGGAG GTCTTGGCGG CCTTGGCGGT 
CTTAGCGGCT CTGGTGGATG GGAGGGATCC AAGTGTCTCA CGGTCCAGGG TCAGGCCGGC 
GCTTGTGTCC GCATCAACAA CTGCCGCCAG TACTACCGAG TGGCCCGCCA GCTGACCTTC 
ATCGCCCTGC GGCAATGGCC CCAGAACCCT CCCCTTGGCC TGGGCGGAAA TATGTGCAAC 
TTTTTCGACC AGCTGGGACG CATCAACAAC GGTATCTGCT GCACGGACAT GGATGCCAAC 
ACTTTCGGAG TATTCCCGCT GCCGACCACA ACGACGACAG AGAGACCTTC GCCGGCTGCC 
GATGAGGGCG AAAATGGGGA TGAGCCAGAG GAGGAGCCAG TCAATGTTGA CACTGTGGTT 
CAACCGGCGG AGACACCACA TCCAGAGGAT CCCATTGATA ATGTGAACAG TGTAGAGGAC 
ACACCGGACT TTCAGGATGT GAACACAATC GAGGCTAATG CCCCAGAACC CGAACCTGAG 
GCGGAACCAG AACTAGAGAC GATACCAGTG CCCGCCCAGC CGGCCACACC CGTCTACCCG 
TATCAGTGGC CATTCGGCTC CTTTGCCGGA GGCCCAGTAG CCCAATGGCC GCCACCGCTG 
CCCACCCATC CGCCTACAAC TGGAGGCTGG CCCCCACCGC TTCCAACCCA TCCGCCCAAC 
CACCACTATC CGACGCATCC TTCGACGTCC GGAGTGCCCA GACCCACCAC AGTGCCCAGC 
ACCAAGCGCA CCACCCCAAG ACCCGCTGCC AGGCCGACTA CCACCAAGCG TCCCACTTAT 
CCCAGCTATC CGGTGACCTC AGCGCCTGCG CCGACGACCA CGCGACGTCC GGTTAGCGGC 
TCCAGTCCCG AGGGCCTGCC GCTGCAGTGT GGCAACAAGA ATCCGGTTTC GCCGGATCAG 
GAGCGCATTG TGGGAGGGAT TAACGCCAGT CCGCACGAGT TCCCCTGGAT AGCGGTGCTA 
TTCAAGTCGG GCAAGCAGTT CTGCGGCGGC AGTCTGATAA CCAACAATCA CATCCTGACA 
GCAGCACATT GTGTGGCACG CATGACCTCG TGGGATGTGG CGGCCTTGAC GGCCCACTTG 
GGCGACTACA ATATCCGGAC GGACTTTGAG GTGCAGCATG TCTCGCGCAG GATCAAACGA 
CTGGTGCGGC ACAAAGGCTT CGAGTTCAGC ACGCTGCACA ACGATGTGGC TATTCTGACT 
CTCAGTGAGC CGGTGCCCTT CACCCACGAG ATCCAGCCGA TTTGCCTGCC AACGTCTCTG 
TCGCAGCAGT CGCGCTCGTA TAGCGGCCAA GTGGCGACTG TGGCCGGCTG GGGCAGTCTG 
CGGGAGAACG GGCCCCAACC CTCGATTCTG CAGAAGGTGG ACATACCCAT TTGGGCGAAT 
GCCGAGTGTG CTCGCAAATA CGGACGTGCA GCGCCCGGTG GCATCATCGA ATCGATGATC 
TGTGCTGGCC AGGCCGCCAA GGATTCCTGC AGCGGCGACT CTGGTGGTCC TATGATCATC 
AACGATGGCG GACGATACAC ACAGGTGGGC ATCGTTTCCT GGGGCATTGG CTGTGGCAAG 
GGTCAATATC CGGGCGTCTA TACGCGTGTC ACGTCGCTGC TGCCCTGGAT CTACAAGAAT 
ATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036731.1
CDS	1582..3330
Translation	MLDPSSASLSRRQRQLFFDPNLFLLQGGLGGLGGLGGLGGLSGSGGWEGSKCLTVQGQAGACVRINNCRQYYRVARQLTFIALRQWPQNPPLGLGGNMCNFFDQLGRINNGICCTDMDANTFGVFPLPTTTTTERPSPAADEGENGDEPEEEPVNVDTVVQPAETPHPEDPIDNVNSVEDTPDFQDVNTIEANAPEPEPEAEPELETIPVPAQPATPVYPYQWPFGSFAGGPVAQWPPPLPTHPPTTGGWPPPLPTHPPNHHYPTHPSTSGVPRPTTVPSTKRTTPRPAARPTTTKRPTYPSYPVTSAPAPTTTRRPVSGSSPEGLPLQCGNKNPVSPDQERIVGGINASPHEFPWIAVLFKSGKQFCGGSLITNNHILTAAHCVARMTSWDVAALTAHLGDYNIRTDFEVQHVSRRIKRLVRHKGFEFSTLHNDVAILTLSEPVPFTHEIQPICLPTSLSQQSRSYSGQVATVAGWGSLRENGPQPSILQKVDIPIWANAECARKYGRAAPGGIIESMICAGQAAKDSCSGDSGGPMIINDGGRYTQVGIVSWGIGCGKGQYPGVYTRVTSLLPWIYKNIK

Target ORF information:

RefSeq Version	XM_015181245.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA16425), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181245.1

ORF Insert Sequence:

ATGTTGGATC CCTCGTCAGC GAGCCTGAGT CGGAGACAGC GTCAACTCTT CTTCGACCCG 
AATCTCTTCC TCCTCCAAGG TGGCCTGGGC GGCCTAGGAG GTCTTGGCGG CCTTGGCGGT 
CTTAGCGGCT CTGGTGGATG GGAGGGATCC AAGTGTCTCA CGGTCCAGGG TCAGGCCGGC 
GCTTGTGTCC GCATCAACAA CTGCCGCCAG TACTACCGAG TGGCCCGCCA GCTGACCTTC 
ATCGCCCTGC GGCAATGGCC CCAGAACCCT CCCCTTGGCC TGGGCGGAAA TATGTGCAAC 
TTTTTCGACC AGCTGGGACG CATCAACAAC GGTATCTGCT GCACGGACAT GGATGCCAAC 
ACTTTCGGAG TATTCCCGCT GCCGACCACA ACGACGACAG AGAGACCTTC GCCGGCTGCC 
GATGAGGGCG AAAATGGGGA TGAGCCAGAG GAGGAGCCAG TCAATGTTGA CACTGTGGTT 
CAACCGGCGG AGACACCACA TCCAGAGGAT CCCATTGATA ATGTGAACAG TGTAGAGGAC 
ACACCGGACT TTCAGGATGT GAACACAATC GAGGCTAATG CCCCAGAACC CGAACCTGAG 
GCGGAACCAG AACTAGAGAC GATACCAGTG CCCGCCCAGC CGGCCACACC CGTCTACCCG 
TATCAGTGGC CATTCGGCTC CTTTGCCGGA GGCCCAGTAG CCCAATGGCC GCCACCGCTG 
CCCACCCATC CGCCTACAAC TGGAGGCTGG CCCCCACCGC TTCCAACCCA TCCGCCCAAC 
CACCACTATC CGACGCATCC TTCGACGTCC GGAGTGCCCA GACCCACCAC AGTGCCCAGC 
ACCAAGCGCA CCACCCCAAG ACCCGCTGCC AGGCCGACTA CCACCAAGCG TCCCACTTAT 
CCCAGCTATC CGGTGACCTC AGCGCCTGCG CCGACGACCA CGCGACGTCC GGTTAGCGGC 
TCCAGTCCCG AGGGCCTGCC GCTGCAGTGT GGCAACAAGA ATCCGGTTTC GCCGGATCAG 
GAGCGCATTG TGGGAGGGAT TAACGCCAGT CCGCACGAGT TCCCCTGGAT AGCGGTGCTA 
TTCAAGTCGG GCAAGCAGTT CTGCGGCGGC AGTCTGATAA CCAACAATCA CATCCTGACA 
GCAGCACATT GTGTGGCACG CATGACCTCG TGGGATGTGG CGGCCTTGAC GGCCCACTTG 
GGCGACTACA ATATCCGGAC GGACTTTGAG GTGCAGCATG TCTCGCGCAG GATCAAACGA 
CTGGTGCGGC ACAAAGGCTT CGAGTTCAGC ACGCTGCACA ACGATGTGGC TATTCTGACT 
CTCAGTGAGC CGGTGCCCTT CACCCACGAG ATCCAGCCGA TTTGCCTGCC AACGTCTCTG 
TCGCAGCAGT CGCGCTCGTA TAGCGGCCAA GTGGCGACTG TGGCCGGCTG GGGCAGTCTG 
CGGGAGAACG GGCCCCAACC CTCGATTCTG CAGAAGGTGG ACATACCCAT TTGGGCGAAT 
GCCGAGTGTG CTCGCAAATA CGGACGTGCA GCGCCCGGTG GCATCATCGA ATCGATGATC 
TGTGCTGGCC AGGCCGCCAA GGATTCCTGC AGCGGCGACT CTGGTGGTCC TATGATCATC 
AACGATGGCG GACGATACAC ACAGGTGGGC ATCGTTTCCT GGGGCATTGG CTGTGGCAAG 
GGTCAATATC CGGGCGTCTA TACGCGTGTC ACGTCGCTGC TGCCCTGGAT CTACAAGAAT 
ATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA16425 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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