Dpse\GA10199 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10199
Entrez Gene ID	4815648
Full Name	GA10199 gene product from transcript GA10199-RA
Synonyms	GA10199,dpse_GLEANR_10809
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015185750.1	XP_015041236.1	uncharacterized protein, isoform B
XM_001355520.2	XP_001355556.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10100	XM_001355520.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe24173	XM_015185750.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10199 ( XM_001355520.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355556.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10199), mRNA.

Price & Availability↑

CloneID	ODe10100
Clone ID Related Accession (Same CDS sequence)	XM_001355520.2
Accession Version	XM_001355520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589961). On Dec 22, 2015 this sequence version replaced XM_001355520.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGA CCGCCTCGGA CAAGTACACG TACCAGCGGG CAGTCCTATG TCTGGCTCGA 
GTGCTGGCTG GGATCCAGCC CACACCGTGG GAGAAGGTGC AGACATTGTT CCGGTATTGC 
CCGCAGGAGA ATGCCGCGGG CGTCTTCTGT CTGGACACTA GGGCCCAGGA TGCGGTCATT 
GCGCTGGGCA TATACTTTCT GGAGAGCGGC TGCCAGCACG AGTCTCAGAT AGTGCCCTAT 
CTGCTGCGGC TGGCCAAGTG CCTGCCGAAG GCGGTCTGGA TCGATGATGC GAGGAGCAGC 
AAGATCGAAC GTGTGCGCAT CCCGTCGGCC GAAAAGTTCA GCTTCTGCCT GAACACTCTG 
CTGTCGGACA TTGCCGCCAA GTGCCCGGAC TCGCGCGAGG AGATCATACT CAATCAGGTG 
GAGACGCTGG GCGCCCTGGC CAACATCGTC AAGTCGAGCA GGGACAGCAG CTCGGCCCCG 
CCGCCGATCA TACTGTGCAA GGCCACAGTT CCGCTGCTGT TTGGACTGGC CCGTTCCATG 
GGCCGCTATG CGAGCAATGA TCCACCGCTG CTGTGCCGCA TCTTTCCGCC GGAGCTGCTG 
CCCATCCAGC GGGTAAGTGG ACGCGACGGC ACCGCCAGCA GCAGTGCCAG CGGCACCTGT 
GGCGGCTCCT TTAGCAGCAG CGAGCGGCTG GCTGCCACTG CCAACCATCA GTTTCGACCG 
ATCATACCCC GCTCCATGTC CGGCAGCCTG GCTGCACAAT ACCACCAGCA CTTTGACGAT 
GGCCGGCAGC GACACCTGGG CTATGGCTTC CAAAGCAATA GCAAGCAGAA GCCGTCCCTG 
AACAGCTATT GCTCGGTTCC GTACGACCCG CGGACGCACT TCTTCACGCG CTACGGCTCC 
AGCTTCAATC AGTTCCCCAA TATGCGGGTC TGCGAATCGC CAACAAAGGG CGGTCCTCGG 
CCCCTGTATC GAGTGCCCCC GTTTCCCATC CAGCATCTGC AGACGATATT CGCGGTGTCC 
AAGAAGCTGC TGACCAAGGA TACGCTGGAG CACCTGGACG AGCAGGCCAG CGACATATTC 
TCGCTGCACC AGATCAAGGG CTACTGCTAC AAAAGCTTCT CGGAGACGCT CAATCTGGTG 
CTGGTCACGT TGCTGCGCGA GCTGTTGCAG CATCAGGTAG ATCTGCCCAC ACCCTTCACC 
AAGGATGTCC AGGAGTTCGT CAAGCGGCTA TTCCTCAACG GCCAGACAGA GCTGCAGAAC 
AAGCAGCAGG ACCAGGAGAG GGAGCGGCGC GAGGAGAACG GCATCACCGT CGTCAACAAG 
TACAAGGTCA ATGTGATGGC CAATGCCGCC TGTGTGGATC TCCTCGTTTG GGCCATACGC 
GATGAGACGG AGGCGGACAA GCTGTGCGGC CGGCTGTCGC AGAAGCTGAA CCTGGTGCTC 
AGCCACAAGA TCGTGATGGA TCACATGCCC CTGCTGATGG TCTGCCTGGA GGGCCTGGGC 
AAGCTGGCCC AGAAGTTCCC CAACATAGCC GGCACATCGA TATCGTATCT GCGCGACTTT 
CTGGTGGACC CGAGCCCCAT TCTGGGGAAG CTGCATGCCC ATGCCATGCA GACGCTGGCC 
CTCCAGAAGA AGGAGAAGGA GCTGACGCCG TTCAAGATCG TGGTCCAGCA TTCGGACTCG 
CGTGCGGTGG TGGACATCCT GACCGATAAC CAGAAGCATG CGGCTGGTGG CGGCCCGAGC 
GGCGGCGGAC GCAGTGGCCA TGTGGCCTTC GAAGCGCTCC GGGACGCGGC CATTGAGAAC 
CTATCGATCG CCCTACGTGC TGCCCATACG CTGGATCAGT TCTGTGTCCC CGCTTTGGTG 
GCCAATGTGT CCAATCGGCT GTTTACCGCC GAGAAGCATG AGAGCGAATC GAATCTGGTG 
AGCCTGAACA TCATCGTGAT GCTGGGCCAT GTGGCCGTGG CATTGAAGGA CACCTCGAAG 
ACCACCCAGA ACATACTGCA GTTCTTCATT CAGCGCTTCT GCAAGGTGCC ATCGGAGCAG 
AACGCCCTGA TTGTCGACCA GCTGGGCTGC ATGATCATCT CCCAGTGCGA GACGCATGTC 
TTCGATGAGA TCATGAAGAT GTTCTCCCGC GTAACAGTGC AGTCGGCCTC GCTGGCGTAC 
ACCTCCGACC CGGAGCACCG GAAAAAGTTC CACCATGTCT CCGATGCGGT GGTGAATGCC 
TTGGGCAATA TAGCGGCCAA CATACAGGGC GACTCCGAGA TGCTGGAGCT GCTGGGCAAG 
CTGCTGGAGC TGTTCGTGCA GATTGGTCTG GACGGCGAGC GCTCCTACGA CAATACGCCG 
GGCGCCCAGA AGGCCAGCTC CCGGGCTGGT AATCTGGGCA TGCTGATACC GGTGATTGCG 
GTACTGGTGC GCCGTCTGCC GCCAATCAAG AATCCCCGCC AGAGGCTGCA CAAGCTCTTC 
AAGGACTTCT GGGCCTACTG TGTGGTCATG GGCTTTACCA ATGCCCGCCT CTGGCCGGCC 
GACTGGTACC AGGGTGTCCA GCAGATAGCC GCCAAATCGC CGCTCCTTAT CTCCCAAACG 
GCCCACAAGT CGGACATGCG GGAGCTCAAC TACACGCTGG CCATCAAGAG CGACTCGGTG 
AACGAGCTGC GCAGCCAGAT ACTGGTCCTG CTCGAGCACT CCTCGGACAA TGTGGCCACG 
GCCATCAACA AGCTAACGTT TGCCCAGTGC ACATACCTCC TGAGCGTCTA CTGGCTGGAG 
ATGCTGCGCG TGGAGAACGC CGACGAGCCC AGTCTGGAGC CCATCATGAG CTATCTGTGC 
GACACGGCCC TGCAGAGGGA CAAAACGGGG ATTTGGCAGT GCGTTAAATG CGTGGCCGAT 
CAGGTGTTTG AGAAGTTCCG CAATGTTCTG TACGCCCACG ATGAGATACG GGAGAAGGTG 
CTCGAGTCGC AGGCCACTCT GCTGCTCGTA TACTTCAATC ACATCCACAA GCAGATCCAA 
CTGGTGGCCG ATCAGTATCT GTCACAGCTG GTGGACCGCT TCCCCCACCT GCTGTGGAAT 
CGTCGCGTGC TCTGGTGCAT GCTGGACATC CTCCAGCTGC TGGCCTACTC GCTGACCCTC 
GATCCCAACG AGGAGACGCC CACGCTGCGA GTGGTTTCGA CGCCCTATAC CCTGCAGCTG 
ATGGACTCGC TGCCGGCGCG CGAGCTGCGG CTGAAGGACT TTGCCGATCG CTGTCAGGGC 
ATTGTCAACG AGGCCATGAA GTGGGCGCCG CGCTCGACGC GCTCCCACCT GCAGGAGTAT 
CCCAACCAGA TACCCACCCC GGCGCTGGCC CACCACAGCG GCCTGGCGCT GGCCATCGAT 
TCGGTGGTCA ACAGCAGCTA CCTCCACCCG GGCAATGCCC TGGGGACGCT CAGCAAGCGC 
CCCAGCTGCG TCAACTCGGA CACGCCCAGG TTCGTGTCGG TTCTGTGCTT GCGGAGCAAG 
TACGCGGGCG AAATCAGCGG CCTGCTGTCG GTGCTCAGTG AGCAGGACAA GGCCGGACTG 
GCCGAGCGAC TGGTCAGCGA CGTGTGGGAG GCCTGCCGGG ACAAGAGCGA TGCCCGTCAC 
CGGGGCGCCC TGTGGCGGGC CACCGCCTAC CTCATAATCT GCGCGGAGGT GGATCGCAAG 
CTGCTGCATG CCGTCGCCAG CTCCCAGCTG GAGCTCTTCA CGGAGTCCGT CATGGAGACG 
GCGGTCGAGT GCTGGCAGTG GGTGCTGACC GCACGGCAGG ACCTCGAGCT GTGCTTCATC 
CAGGAGATGG TCAGTGCCTG GCAGACGACC TTCGAGAAGC GCATGGGTCT GTTTGCCCTC 
GAGCAGGAGG TCACCCATCC ATTGGCCGCC TACGAGGGCT GCAAGCTCGT GAGCAGCCCC 
ATCCTAATTG TCCCGCACCT CATCTGGCTG CAGCTGCTCT CGGAGATGGT GGACACGGCC 
AAATACTGCA ACCGGGACAA GGTGGAGATG TTCTGCCTGC TCCTGCACCG CTGCCTGCCC 
ATTCTGAAGA GCAGCCGACA GAACCGGCAG GTGGCCACCG TCGGCTGTCG CTTCAAGCTG 
CTCCAGTGCG GCCTCTCGCT GCTGCAGGGC AACACCATAC CCAAGTCGCT GGCGCGGAAC 
ATATTGCGCG AGAGGATCTA CTCGAATGCC CTCGACTACT TCTGCGGCCC GCCCACCTGC 
CCCGTCCAGA GCAGGGACCA GCTGCTGGAC GACATAATGA TTCTGCTCAA GTTCTGGCAG 
ACGATGCGCA GCGAGAAGAA GCACTTGGTC ACCTCCGAGG TGGGCGACTA TGACATGGCG 
GGCCACGCCT CGGGCAGCTC CAACCAAATG CTGGGGGTCA AGGCCAATCC GGAGACGGCC 
TCGCTCATCA GCGGCGGGGC CGACTACTCG ATGCGATCGA TGAGCGCCTC TGGGAATGTG 
ACTGGTGGCA GCAGCGGCTG GTACAACACC ATACCCCACT CGACGTCGAC GCTGTCGAAG 
CGCTCGAATC GCTCCAAGCG GCTGCAGTAC CAGAAGGACT CCTACGACAA GGACTACATG 
AAGAAGCGCA ATCTGATACT CGAGCTGCTG GCCGTCGAGC TGGAGTTCCT CATCACCTGG 
TACAATCCCA ATAGTCTGCC CGATCTGATA GTGCCAGGCG AGGAACAGAT CACCGAGTGG 
CGCAATCGTC CGTACAAGGT GAACGTGTGG CGGGACTATG CCCGCCTGGC CTGGTGCTAC 
AATCCCTCGC TGGCCGTCTT CCTGCCGCAG CGTATCAAGA ACGCAGAGAT CATCGACGAG 
GAGGTATCCC GCCTCGTCTG TTCCGATCCG ATAGCCGTCT GTCATATACC GGAGGCCCTC 
AAGTATCTGT GCACCACCAA GAATCTGCTG CAGGAGAGCC CCGATCTGGT GTACATACTC 
TCCTGGTCGC CCGTCACCCC CATCCAGGCG CTGTCGTACT TTTCGCGCCA GTATCCGTCG 
CATCCGCTGA CCGCCCAGTA CGCAGTCAAG TCGCTCTCCT CGTTCCCCGC CGAGTCCGTG 
CTGCCGTACA TTCCCCAACT GGTGCAGGCC CTGCGCCACG ACACCATGGG CTATGTGGTG 
GAGTTCATCA AGAACATCTC GAAGCGTTCG CAGATCGTCG CCCACCAGCT CATCTGGAAC 
ATGCAGACGA ATATGTATAT GGACGAGGAC CAGATGCATC GCGATCCCAA TCTGTACGAG 
GCGCTGGATC TGCTCTCGCA GAACATCATT GCCTCGTTCT CGGGCGCCGC GAAGCGATTC 
TATGAGCGGG AATTCGATTT CTTTGGCAAA ATAACAGCCG TCTCTGGCGA GATACGATCC 
TTTGCCAAGG GCATGGAGCG AAAGAATGCC TGTTTGGCGG CCCTTAGCCG CATCAAGGTG 
CAGTCGGGCT GTTATCTGCC CTCGAATCCA GAGGCCATGG TCCTGGACAT TGACTACAGC 
AGCGGCACGC CCATGCAGAG TGCGGCCAAA GCGCCGTATC TGGCCCGTTT TCGTGTCTAT 
CGGTGCGGCA TCACCGAGCT GGAGACCCGA GCCATGGAGG TGTCCAATAA TCCGAACTCC 
CAGGAGGATG CCAAGATAAC GCTGGGCGTC GAGTCGTGGC AGGCGGCCAT CTTCAAGGTG 
GGCGACGATG TGCGCCAGGA TATGCTGGCG CTTCAGGTGA TCACCATATT CAAGAATATC 
TTCCAACAAG TCGGCCTCGA TCTGTTTCTG TTTCCCTACC GCGTCGTGGC CACAGCTCCA 
GGCTGCGGCG TGATCGAGTG CGTGCCGAAT GCCAAGTCAC GCGATCAGTT GGGCAGGCAG 
ACAGACTCGG GCCTATCCGA GTACTTCCAG CATCAGTATG GCGACGAGAG CTCCAAGGAG 
TTTCAGGCGG CGCGGGCGAA CTTTGTGAAA TCGATGGCCG CCTACTCGCT GATCGGGTAT 
CTGCTGCAGA TCAAGGATCG CCACAATGGC AACATCATGA TCGACAAGGA TGGCCACATC 
ATACACATCG ATTTCGGCTT TATGTTCGAG TCATCGCCCG GTGGCAATAT CGGCTTTGAG 
CCCGACATGA AGCTCACGGA TGAGATGGTC ATGATTATGG GCGGCAAAAT GGACTCGCCC 
GCCTTCAAAT GGTTCTGCGA GCTGTGCGTG CAGGCCTTCC TGGCGGTGCG CCCCTACCAG 
GATGCGATTG TGTCTCTGGT CTCGCTGATG CTCGACACGG GGCTGCCCTG CTTCCGCGGA 
CAGACGATCA ATCTGCTCAA GCAGCGGTTC GTGGCCACCA AGAACAACAA GGAGGCCGCC 
GCCCACATGC TCTCCGTCAT ACGAAACTCT TATCAGAATT TCCGCACGCG TACCTACGAC 
ATGATCCAGT ACTATCAGAA CCAGATACCC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355556.1
CDS	263..6658
Translation	MTMTASDKYTYQRAVLCLARVLAGIQPTPWEKVQTLFRYCPQENAAGVFCLDTRAQDAVIALGIYFLESGCQHESQIVPYLLRLAKCLPKAVWIDDARSSKIERVRIPSAEKFSFCLNTLLSDIAAKCPDSREEIILNQVETLGALANIVKSSRDSSSAPPPIILCKATVPLLFGLARSMGRYASNDPPLLCRIFPPELLPIQRVSGRDGTASSSASGTCGGSFSSSERLAATANHQFRPIIPRSMSGSLAAQYHQHFDDGRQRHLGYGFQSNSKQKPSLNSYCSVPYDPRTHFFTRYGSSFNQFPNMRVCESPTKGGPRPLYRVPPFPIQHLQTIFAVSKKLLTKDTLEHLDEQASDIFSLHQIKGYCYKSFSETLNLVLVTLLRELLQHQVDLPTPFTKDVQEFVKRLFLNGQTELQNKQQDQERERREENGITVVNKYKVNVMANAACVDLLVWAIRDETEADKLCGRLSQKLNLVLSHKIVMDHMPLLMVCLEGLGKLAQKFPNIAGTSISYLRDFLVDPSPILGKLHAHAMQTLALQKKEKELTPFKIVVQHSDSRAVVDILTDNQKHAAGGGPSGGGRSGHVAFEALRDAAIENLSIALRAAHTLDQFCVPALVANVSNRLFTAEKHESESNLVSLNIIVMLGHVAVALKDTSKTTQNILQFFIQRFCKVPSEQNALIVDQLGCMIISQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKKFHHVSDAVVNALGNIAANIQGDSEMLELLGKLLELFVQIGLDGERSYDNTPGAQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLFKDFWAYCVVMGFTNARLWPADWYQGVQQIAAKSPLLISQTAHKSDMRELNYTLAIKSDSVNELRSQILVLLEHSSDNVATAINKLTFAQCTYLLSVYWLEMLRVENADEPSLEPIMSYLCDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEIREKVLESQATLLLVYFNHIHKQIQLVADQYLSQLVDRFPHLLWNRRVLWCMLDILQLLAYSLTLDPNEETPTLRVVSTPYTLQLMDSLPARELRLKDFADRCQGIVNEAMKWAPRSTRSHLQEYPNQIPTPALAHHSGLALAIDSVVNSSYLHPGNALGTLSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEQDKAGLAERLVSDVWEACRDKSDARHRGALWRATAYLIICAEVDRKLLHAVASSQLELFTESVMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFALEQEVTHPLAAYEGCKLVSSPILIVPHLIWLQLLSEMVDTAKYCNRDKVEMFCLLLHRCLPILKSSRQNRQVATVGCRFKLLQCGLSLLQGNTIPKSLARNILRERIYSNALDYFCGPPTCPVQSRDQLLDDIMILLKFWQTMRSEKKHLVTSEVGDYDMAGHASGSSNQMLGVKANPETASLISGGADYSMRSMSASGNVTGGSSGWYNTIPHSTSTLSKRSNRSKRLQYQKDSYDKDYMKKRNLILELLAVELEFLITWYNPNSLPDLIVPGEEQITEWRNRPYKVNVWRDYARLAWCYNPSLAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALSYFSRQYPSHPLTAQYAVKSLSSFPAESVLPYIPQLVQALRHDTMGYVVEFIKNISKRSQIVAHQLIWNMQTNMYMDEDQMHRDPNLYEALDLLSQNIIASFSGAAKRFYEREFDFFGKITAVSGEIRSFAKGMERKNACLAALSRIKVQSGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRCGITELETRAMEVSNNPNSQEDAKITLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGLSEYFQHQYGDESSKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFGFMFESSPGGNIGFEPDMKLTDEMVMIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRGQTINLLKQRFVATKNNKEAAAHMLSVIRNSYQNFRTRTYDMIQYYQNQIPY

Target ORF information:

RefSeq Version	XM_001355520.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355520.2

ORF Insert Sequence:

ATGACGATGA CCGCCTCGGA CAAGTACACG TACCAGCGGG CAGTCCTATG TCTGGCTCGA 
GTGCTGGCTG GGATCCAGCC CACACCGTGG GAGAAGGTGC AGACATTGTT CCGGTATTGC 
CCGCAGGAGA ATGCCGCGGG CGTCTTCTGT CTGGACACTA GGGCCCAGGA TGCGGTCATT 
GCGCTGGGCA TATACTTTCT GGAGAGCGGC TGCCAGCACG AGTCTCAGAT AGTGCCCTAT 
CTGCTGCGGC TGGCCAAGTG CCTGCCGAAG GCGGTCTGGA TCGATGATGC GAGGAGCAGC 
AAGATCGAAC GTGTGCGCAT CCCGTCGGCC GAAAAGTTCA GCTTCTGCCT GAACACTCTG 
CTGTCGGACA TTGCCGCCAA GTGCCCGGAC TCGCGCGAGG AGATCATACT CAATCAGGTG 
GAGACGCTGG GCGCCCTGGC CAACATCGTC AAGTCGAGCA GGGACAGCAG CTCGGCCCCG 
CCGCCGATCA TACTGTGCAA GGCCACAGTT CCGCTGCTGT TTGGACTGGC CCGTTCCATG 
GGCCGCTATG CGAGCAATGA TCCACCGCTG CTGTGCCGCA TCTTTCCGCC GGAGCTGCTG 
CCCATCCAGC GGGTAAGTGG ACGCGACGGC ACCGCCAGCA GCAGTGCCAG CGGCACCTGT 
GGCGGCTCCT TTAGCAGCAG CGAGCGGCTG GCTGCCACTG CCAACCATCA GTTTCGACCG 
ATCATACCCC GCTCCATGTC CGGCAGCCTG GCTGCACAAT ACCACCAGCA CTTTGACGAT 
GGCCGGCAGC GACACCTGGG CTATGGCTTC CAAAGCAATA GCAAGCAGAA GCCGTCCCTG 
AACAGCTATT GCTCGGTTCC GTACGACCCG CGGACGCACT TCTTCACGCG CTACGGCTCC 
AGCTTCAATC AGTTCCCCAA TATGCGGGTC TGCGAATCGC CAACAAAGGG CGGTCCTCGG 
CCCCTGTATC GAGTGCCCCC GTTTCCCATC CAGCATCTGC AGACGATATT CGCGGTGTCC 
AAGAAGCTGC TGACCAAGGA TACGCTGGAG CACCTGGACG AGCAGGCCAG CGACATATTC 
TCGCTGCACC AGATCAAGGG CTACTGCTAC AAAAGCTTCT CGGAGACGCT CAATCTGGTG 
CTGGTCACGT TGCTGCGCGA GCTGTTGCAG CATCAGGTAG ATCTGCCCAC ACCCTTCACC 
AAGGATGTCC AGGAGTTCGT CAAGCGGCTA TTCCTCAACG GCCAGACAGA GCTGCAGAAC 
AAGCAGCAGG ACCAGGAGAG GGAGCGGCGC GAGGAGAACG GCATCACCGT CGTCAACAAG 
TACAAGGTCA ATGTGATGGC CAATGCCGCC TGTGTGGATC TCCTCGTTTG GGCCATACGC 
GATGAGACGG AGGCGGACAA GCTGTGCGGC CGGCTGTCGC AGAAGCTGAA CCTGGTGCTC 
AGCCACAAGA TCGTGATGGA TCACATGCCC CTGCTGATGG TCTGCCTGGA GGGCCTGGGC 
AAGCTGGCCC AGAAGTTCCC CAACATAGCC GGCACATCGA TATCGTATCT GCGCGACTTT 
CTGGTGGACC CGAGCCCCAT TCTGGGGAAG CTGCATGCCC ATGCCATGCA GACGCTGGCC 
CTCCAGAAGA AGGAGAAGGA GCTGACGCCG TTCAAGATCG TGGTCCAGCA TTCGGACTCG 
CGTGCGGTGG TGGACATCCT GACCGATAAC CAGAAGCATG CGGCTGGTGG CGGCCCGAGC 
GGCGGCGGAC GCAGTGGCCA TGTGGCCTTC GAAGCGCTCC GGGACGCGGC CATTGAGAAC 
CTATCGATCG CCCTACGTGC TGCCCATACG CTGGATCAGT TCTGTGTCCC CGCTTTGGTG 
GCCAATGTGT CCAATCGGCT GTTTACCGCC GAGAAGCATG AGAGCGAATC GAATCTGGTG 
AGCCTGAACA TCATCGTGAT GCTGGGCCAT GTGGCCGTGG CATTGAAGGA CACCTCGAAG 
ACCACCCAGA ACATACTGCA GTTCTTCATT CAGCGCTTCT GCAAGGTGCC ATCGGAGCAG 
AACGCCCTGA TTGTCGACCA GCTGGGCTGC ATGATCATCT CCCAGTGCGA GACGCATGTC 
TTCGATGAGA TCATGAAGAT GTTCTCCCGC GTAACAGTGC AGTCGGCCTC GCTGGCGTAC 
ACCTCCGACC CGGAGCACCG GAAAAAGTTC CACCATGTCT CCGATGCGGT GGTGAATGCC 
TTGGGCAATA TAGCGGCCAA CATACAGGGC GACTCCGAGA TGCTGGAGCT GCTGGGCAAG 
CTGCTGGAGC TGTTCGTGCA GATTGGTCTG GACGGCGAGC GCTCCTACGA CAATACGCCG 
GGCGCCCAGA AGGCCAGCTC CCGGGCTGGT AATCTGGGCA TGCTGATACC GGTGATTGCG 
GTACTGGTGC GCCGTCTGCC GCCAATCAAG AATCCCCGCC AGAGGCTGCA CAAGCTCTTC 
AAGGACTTCT GGGCCTACTG TGTGGTCATG GGCTTTACCA ATGCCCGCCT CTGGCCGGCC 
GACTGGTACC AGGGTGTCCA GCAGATAGCC GCCAAATCGC CGCTCCTTAT CTCCCAAACG 
GCCCACAAGT CGGACATGCG GGAGCTCAAC TACACGCTGG CCATCAAGAG CGACTCGGTG 
AACGAGCTGC GCAGCCAGAT ACTGGTCCTG CTCGAGCACT CCTCGGACAA TGTGGCCACG 
GCCATCAACA AGCTAACGTT TGCCCAGTGC ACATACCTCC TGAGCGTCTA CTGGCTGGAG 
ATGCTGCGCG TGGAGAACGC CGACGAGCCC AGTCTGGAGC CCATCATGAG CTATCTGTGC 
GACACGGCCC TGCAGAGGGA CAAAACGGGG ATTTGGCAGT GCGTTAAATG CGTGGCCGAT 
CAGGTGTTTG AGAAGTTCCG CAATGTTCTG TACGCCCACG ATGAGATACG GGAGAAGGTG 
CTCGAGTCGC AGGCCACTCT GCTGCTCGTA TACTTCAATC ACATCCACAA GCAGATCCAA 
CTGGTGGCCG ATCAGTATCT GTCACAGCTG GTGGACCGCT TCCCCCACCT GCTGTGGAAT 
CGTCGCGTGC TCTGGTGCAT GCTGGACATC CTCCAGCTGC TGGCCTACTC GCTGACCCTC 
GATCCCAACG AGGAGACGCC CACGCTGCGA GTGGTTTCGA CGCCCTATAC CCTGCAGCTG 
ATGGACTCGC TGCCGGCGCG CGAGCTGCGG CTGAAGGACT TTGCCGATCG CTGTCAGGGC 
ATTGTCAACG AGGCCATGAA GTGGGCGCCG CGCTCGACGC GCTCCCACCT GCAGGAGTAT 
CCCAACCAGA TACCCACCCC GGCGCTGGCC CACCACAGCG GCCTGGCGCT GGCCATCGAT 
TCGGTGGTCA ACAGCAGCTA CCTCCACCCG GGCAATGCCC TGGGGACGCT CAGCAAGCGC 
CCCAGCTGCG TCAACTCGGA CACGCCCAGG TTCGTGTCGG TTCTGTGCTT GCGGAGCAAG 
TACGCGGGCG AAATCAGCGG CCTGCTGTCG GTGCTCAGTG AGCAGGACAA GGCCGGACTG 
GCCGAGCGAC TGGTCAGCGA CGTGTGGGAG GCCTGCCGGG ACAAGAGCGA TGCCCGTCAC 
CGGGGCGCCC TGTGGCGGGC CACCGCCTAC CTCATAATCT GCGCGGAGGT GGATCGCAAG 
CTGCTGCATG CCGTCGCCAG CTCCCAGCTG GAGCTCTTCA CGGAGTCCGT CATGGAGACG 
GCGGTCGAGT GCTGGCAGTG GGTGCTGACC GCACGGCAGG ACCTCGAGCT GTGCTTCATC 
CAGGAGATGG TCAGTGCCTG GCAGACGACC TTCGAGAAGC GCATGGGTCT GTTTGCCCTC 
GAGCAGGAGG TCACCCATCC ATTGGCCGCC TACGAGGGCT GCAAGCTCGT GAGCAGCCCC 
ATCCTAATTG TCCCGCACCT CATCTGGCTG CAGCTGCTCT CGGAGATGGT GGACACGGCC 
AAATACTGCA ACCGGGACAA GGTGGAGATG TTCTGCCTGC TCCTGCACCG CTGCCTGCCC 
ATTCTGAAGA GCAGCCGACA GAACCGGCAG GTGGCCACCG TCGGCTGTCG CTTCAAGCTG 
CTCCAGTGCG GCCTCTCGCT GCTGCAGGGC AACACCATAC CCAAGTCGCT GGCGCGGAAC 
ATATTGCGCG AGAGGATCTA CTCGAATGCC CTCGACTACT TCTGCGGCCC GCCCACCTGC 
CCCGTCCAGA GCAGGGACCA GCTGCTGGAC GACATAATGA TTCTGCTCAA GTTCTGGCAG 
ACGATGCGCA GCGAGAAGAA GCACTTGGTC ACCTCCGAGG TGGGCGACTA TGACATGGCG 
GGCCACGCCT CGGGCAGCTC CAACCAAATG CTGGGGGTCA AGGCCAATCC GGAGACGGCC 
TCGCTCATCA GCGGCGGGGC CGACTACTCG ATGCGATCGA TGAGCGCCTC TGGGAATGTG 
ACTGGTGGCA GCAGCGGCTG GTACAACACC ATACCCCACT CGACGTCGAC GCTGTCGAAG 
CGCTCGAATC GCTCCAAGCG GCTGCAGTAC CAGAAGGACT CCTACGACAA GGACTACATG 
AAGAAGCGCA ATCTGATACT CGAGCTGCTG GCCGTCGAGC TGGAGTTCCT CATCACCTGG 
TACAATCCCA ATAGTCTGCC CGATCTGATA GTGCCAGGCG AGGAACAGAT CACCGAGTGG 
CGCAATCGTC CGTACAAGGT GAACGTGTGG CGGGACTATG CCCGCCTGGC CTGGTGCTAC 
AATCCCTCGC TGGCCGTCTT CCTGCCGCAG CGTATCAAGA ACGCAGAGAT CATCGACGAG 
GAGGTATCCC GCCTCGTCTG TTCCGATCCG ATAGCCGTCT GTCATATACC GGAGGCCCTC 
AAGTATCTGT GCACCACCAA GAATCTGCTG CAGGAGAGCC CCGATCTGGT GTACATACTC 
TCCTGGTCGC CCGTCACCCC CATCCAGGCG CTGTCGTACT TTTCGCGCCA GTATCCGTCG 
CATCCGCTGA CCGCCCAGTA CGCAGTCAAG TCGCTCTCCT CGTTCCCCGC CGAGTCCGTG 
CTGCCGTACA TTCCCCAACT GGTGCAGGCC CTGCGCCACG ACACCATGGG CTATGTGGTG 
GAGTTCATCA AGAACATCTC GAAGCGTTCG CAGATCGTCG CCCACCAGCT CATCTGGAAC 
ATGCAGACGA ATATGTATAT GGACGAGGAC CAGATGCATC GCGATCCCAA TCTGTACGAG 
GCGCTGGATC TGCTCTCGCA GAACATCATT GCCTCGTTCT CGGGCGCCGC GAAGCGATTC 
TATGAGCGGG AATTCGATTT CTTTGGCAAA ATAACAGCCG TCTCTGGCGA GATACGATCC 
TTTGCCAAGG GCATGGAGCG AAAGAATGCC TGTTTGGCGG CCCTTAGCCG CATCAAGGTG 
CAGTCGGGCT GTTATCTGCC CTCGAATCCA GAGGCCATGG TCCTGGACAT TGACTACAGC 
AGCGGCACGC CCATGCAGAG TGCGGCCAAA GCGCCGTATC TGGCCCGTTT TCGTGTCTAT 
CGGTGCGGCA TCACCGAGCT GGAGACCCGA GCCATGGAGG TGTCCAATAA TCCGAACTCC 
CAGGAGGATG CCAAGATAAC GCTGGGCGTC GAGTCGTGGC AGGCGGCCAT CTTCAAGGTG 
GGCGACGATG TGCGCCAGGA TATGCTGGCG CTTCAGGTGA TCACCATATT CAAGAATATC 
TTCCAACAAG TCGGCCTCGA TCTGTTTCTG TTTCCCTACC GCGTCGTGGC CACAGCTCCA 
GGCTGCGGCG TGATCGAGTG CGTGCCGAAT GCCAAGTCAC GCGATCAGTT GGGCAGGCAG 
ACAGACTCGG GCCTATCCGA GTACTTCCAG CATCAGTATG GCGACGAGAG CTCCAAGGAG 
TTTCAGGCGG CGCGGGCGAA CTTTGTGAAA TCGATGGCCG CCTACTCGCT GATCGGGTAT 
CTGCTGCAGA TCAAGGATCG CCACAATGGC AACATCATGA TCGACAAGGA TGGCCACATC 
ATACACATCG ATTTCGGCTT TATGTTCGAG TCATCGCCCG GTGGCAATAT CGGCTTTGAG 
CCCGACATGA AGCTCACGGA TGAGATGGTC ATGATTATGG GCGGCAAAAT GGACTCGCCC 
GCCTTCAAAT GGTTCTGCGA GCTGTGCGTG CAGGCCTTCC TGGCGGTGCG CCCCTACCAG 
GATGCGATTG TGTCTCTGGT CTCGCTGATG CTCGACACGG GGCTGCCCTG CTTCCGCGGA 
CAGACGATCA ATCTGCTCAA GCAGCGGTTC GTGGCCACCA AGAACAACAA GGAGGCCGCC 
GCCCACATGC TCTCCGTCAT ACGAAACTCT TATCAGAATT TCCGCACGCG TACCTACGAC 
ATGATCCAGT ACTATCAGAA CCAGATACCC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10199 ( XM_015185750.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015041236.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10199), mRNA.

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CloneID	ODe24173
Clone ID Related Accession (Same CDS sequence)	XM_015185750.1
Accession Version	XM_015185750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589961). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGA CCGCCTCGGA CAAGTACACG TACCAGCGGG CAGTCCTATG TCTGGCTCGA 
GTGCTGGCTG GGATCCAGCC CACACCGTGG GAGAAGGTGC AGACATTGTT CCGGTATTGC 
CCGCAGGAGA ATGCCGCGGG CGTCTTCTGT CTGGACACTA GGGCCCAGGA TGCGGTCATT 
GCGCTGGGCA TATACTTTCT GGAGAGCGGC TGCCAGCACG AGTCTCAGAT AGTGCCCTAT 
CTGCTGCGGC TGGCCAAGTG CCTGCCGAAG GCGGTCTGGA TCGATGATGC GAGGAGCAGC 
AAGATCGAAC GTGTGCGCAT CCCGTCGGCC GAAAAGTTCA GCTTCTGCCT GAACACTCTG 
CTGTCGGACA TTGCCGCCAA GTGCCCGGAC TCGCGCGAGG AGATCATACT CAATCAGGTG 
GAGACGCTGG GCGCCCTGGC CAACATCGTC AAGTCGAGCA GGGACAGCAG CTCGGCCCCG 
CCGCCGATCA TACTGTGCAA GGCCACAGTT CCGCTGCTGT TTGGACTGGC CCGTTCCATG 
GGCCGCTATG CGAGCAATGA TCCACCGCTG CTGTGCCGCA TCTTTCCGCC GGAGCTGCTG 
CCCATCCAGC GGGTAAGTGG ACGCGACGGC ACCGCCAGCA GCAGTGCCAG CGGCACCTGT 
GGCGGCTCCT TTAGCAGCAG CGAGCGGCTG GCTGCCACTG CCAACCATCA GTTTCGACCG 
ATCATACCCC GCTCCATGTC CGGCAGCCTG GCTGCACAAT ACCACCAGCA CTTTGACGAT 
GGCCGGCAGC GACACCTGGG CTATGGCTTC CAAAGCAATA GCAAGCAGAA GCCGTCCCTG 
AACAGCTATT GCTCGGTTCC GTACGACCCG CGGACGCACT TCTTCACGCG CTACGGCTCC 
AGCTTCAATC AGTTCCCCAA TATGCGGGTC TGCGAATCGC CAACAAAGGG CGGTCCTCGG 
CCCCTGTATC GAGTGCCCCC GTTTCCCATC CAGCATCTGC AGACGATATT CGCGGTGTCC 
AAGAAGCTGC TGACCAAGGA TACGCTGGAG CACCTGGACG AGCAGGCCAG CGACATATTC 
TCGCTGCACC AGATCAAGGG CTACTGCTAC AAAAGCTTCT CGGAGACGCT CAATCTGGTG 
CTGGTCACGT TGCTGCGCGA GCTGTTGCAG CATCAGGTAG ATCTGCCCAC ACCCTTCACC 
AAGGATGTCC AGGAGTTCGT CAAGCGGCTA TTCCTCAACG GCCAGACAGA GCTGCAGAAC 
AAGCAGCAGG ACCAGGAGAG GGAGCGGCGC GAGGAGAACG GCATCACCGT CGTCAACAAG 
TACAAGGTCA ATGTGATGGC CAATGCCGCC TGTGTGGATC TCCTCGTTTG GGCCATACGC 
GATGAGACGG TTGATGTCGA CTATACGGTG CCATCCCTGC AGAATGGCTT GCAATTTTTA 
TTGAAGAAAG AGGCGGACAA GCTGTGCGGC CGGCTGTCGC AGAAGCTGAA CCTGGTGCTC 
AGCCACAAGA TCGTGATGGA TCACATGCCC CTGCTGATGG TCTGCCTGGA GGGCCTGGGC 
AAGCTGGCCC AGAAGTTCCC CAACATAGCC GGCACATCGA TATCGTATCT GCGCGACTTT 
CTGGTGGACC CGAGCCCCAT TCTGGGGAAG CTGCATGCCC ATGCCATGCA GACGCTGGCC 
CTCCAGAAGA AGGAGAAGGA GCTGACGCCG TTCAAGATCG TGGTCCAGCA TTCGGACTCG 
CGTGCGGTGG TGGACATCCT GACCGATAAC CAGAAGCATG CGGCTGGTGG CGGCCCGAGC 
GGCGGCGGAC GCAGTGGCCA TGTGGCCTTC GAAGCGCTCC GGGACGCGGC CATTGAGAAC 
CTATCGATCG CCCTACGTGC TGCCCATACG CTGGATCAGT TCTGTGTCCC CGCTTTGGTG 
GCCAATGTGT CCAATCGGCT GTTTACCGCC GAGAAGCATG AGAGCGAATC GAATCTGGTG 
AGCCTGAACA TCATCGTGAT GCTGGGCCAT GTGGCCGTGG CATTGAAGGA CACCTCGAAG 
ACCACCCAGA ACATACTGCA GTTCTTCATT CAGCGCTTCT GCAAGGTGCC ATCGGAGCAG 
AACGCCCTGA TTGTCGACCA GCTGGGCTGC ATGATCATCT CCCAGTGCGA GACGCATGTC 
TTCGATGAGA TCATGAAGAT GTTCTCCCGC GTAACAGTGC AGTCGGCCTC GCTGGCGTAC 
ACCTCCGACC CGGAGCACCG GAAAAAGTTC CACCATGTCT CCGATGCGGT GGTGAATGCC 
TTGGGCAATA TAGCGGCCAA CATACAGGGC GACTCCGAGA TGCTGGAGCT GCTGGGCAAG 
CTGCTGGAGC TGTTCGTGCA GATTGGTCTG GACGGCGAGC GCTCCTACGA CAATACGCCG 
GGCGCCCAGA AGGCCAGCTC CCGGGCTGGT AATCTGGGCA TGCTGATACC GGTGATTGCG 
GTACTGGTGC GCCGTCTGCC GCCAATCAAG AATCCCCGCC AGAGGCTGCA CAAGCTCTTC 
AAGGACTTCT GGGCCTACTG TGTGGTCATG GGCTTTACCA ATGCCCGCCT CTGGCCGGCC 
GACTGGTACC AGGGTGTCCA GCAGATAGCC GCCAAATCGC CGCTCCTTAT CTCCCAAACG 
GCCCACAAGT CGGACATGCG GGAGCTCAAC TACACGCTGG CCATCAAGAG CGACTCGGTG 
AACGAGCTGC GCAGCCAGAT ACTGGTCCTG CTCGAGCACT CCTCGGACAA TGTGGCCACG 
GCCATCAACA AGCTAACGTT TGCCCAGTGC ACATACCTCC TGAGCGTCTA CTGGCTGGAG 
ATGCTGCGCG TGGAGAACGC CGACGAGCCC AGTCTGGAGC CCATCATGAG CTATCTGTGC 
GACACGGCCC TGCAGAGGGA CAAAACGGGG ATTTGGCAGT GCGTTAAATG CGTGGCCGAT 
CAGGTGTTTG AGAAGTTCCG CAATGTTCTG TACGCCCACG ATGAGATACG GGAGAAGGTG 
CTCGAGTCGC AGGCCACTCT GCTGCTCGTA TACTTCAATC ACATCCACAA GCAGATCCAA 
CTGGTGGCCG ATCAGTATCT GTCACAGCTG GTGGACCGCT TCCCCCACCT GCTGTGGAAT 
CGTCGCGTGC TCTGGTGCAT GCTGGACATC CTCCAGCTGC TGGCCTACTC GCTGACCCTC 
GATCCCAACG AGGAGACGCC CACGCTGCGA GTGGTTTCGA CGCCCTATAC CCTGCAGCTG 
ATGGACTCGC TGCCGGCGCG CGAGCTGCGG CTGAAGGACT TTGCCGATCG CTGTCAGGGC 
ATTGTCAACG AGGCCATGAA GTGGGCGCCG CGCTCGACGC GCTCCCACCT GCAGGAGTAT 
CCCAACCAGA TACCCACCCC GGCGCTGGCC CACCACAGCG GCCTGGCGCT GGCCATCGAT 
TCGGTGGTCA ACAGCAGCTA CCTCCACCCG GGCAATGCCC TGGGGACGCT CAGCAAGCGC 
CCCAGCTGCG TCAACTCGGA CACGCCCAGG TTCGTGTCGG TTCTGTGCTT GCGGAGCAAG 
TACGCGGGCG AAATCAGCGG CCTGCTGTCG GTGCTCAGTG AGCAGGACAA GGCCGGACTG 
GCCGAGCGAC TGGTCAGCGA CGTGTGGGAG GCCTGCCGGG ACAAGAGCGA TGCCCGTCAC 
CGGGGCGCCC TGTGGCGGGC CACCGCCTAC CTCATAATCT GCGCGGAGGT GGATCGCAAG 
CTGCTGCATG CCGTCGCCAG CTCCCAGCTG GAGCTCTTCA CGGAGTCCGT CATGGAGACG 
GCGGTCGAGT GCTGGCAGTG GGTGCTGACC GCACGGCAGG ACCTCGAGCT GTGCTTCATC 
CAGGAGATGG TCAGTGCCTG GCAGACGACC TTCGAGAAGC GCATGGGTCT GTTTGCCCTC 
GAGCAGGAGG TCACCCATCC ATTGGCCGCC TACGAGGGCT GCAAGCTCGT GAGCAGCCCC 
ATCCTAATTG TCCCGCACCT CATCTGGCTG CAGCTGCTCT CGGAGATGGT GGACACGGCC 
AAATACTGCA ACCGGGACAA GGTGGAGATG TTCTGCCTGC TCCTGCACCG CTGCCTGCCC 
ATTCTGAAGA GCAGCCGACA GAACCGGCAG GTGGCCACCG TCGGCTGTCG CTTCAAGCTG 
CTCCAGTGCG GCCTCTCGCT GCTGCAGGGC AACACCATAC CCAAGTCGCT GGCGCGGAAC 
ATATTGCGCG AGAGGATCTA CTCGAATGCC CTCGACTACT TCTGCGGCCC GCCCACCTGC 
CCCGTCCAGA GCAGGGACCA GCTGCTGGAC GACATAATGA TTCTGCTCAA GTTCTGGCAG 
ACGATGCGCA GCGAGAAGAA GCACTTGGTC ACCTCCGAGG TGGGCGACTA TGACATGGCG 
GGCCACGCCT CGGGCAGCTC CAACCAAATG CTGGGGGTCA AGGCCAATCC GGAGACGGCC 
TCGCTCATCA GCGGCGGGGC CGACTACTCG ATGCGATCGA TGAGCGCCTC TGGGAATGTG 
ACTGGTGGCA GCAGCGGCTG GTACAACACC ATACCCCACT CGACGTCGAC GCTGTCGAAG 
CGCTCGAATC GCTCCAAGCG GCTGCAGTAC CAGAAGGACT CCTACGACAA GGACTACATG 
AAGAAGCGCA ATCTGATACT CGAGCTGCTG GCCGTCGAGC TGGAGTTCCT CATCACCTGG 
TACAATCCCA ATAGTCTGCC CGATCTGATA GTGCCAGGCG AGGAACAGAT CACCGAGTGG 
CGCAATCGTC CGTACAAGGT GAACGTGTGG CGGGACTATG CCCGCCTGGC CTGGTGCTAC 
AATCCCTCGC TGGCCGTCTT CCTGCCGCAG CGTATCAAGA ACGCAGAGAT CATCGACGAG 
GAGGTATCCC GCCTCGTCTG TTCCGATCCG ATAGCCGTCT GTCATATACC GGAGGCCCTC 
AAGTATCTGT GCACCACCAA GAATCTGCTG CAGGAGAGCC CCGATCTGGT GTACATACTC 
TCCTGGTCGC CCGTCACCCC CATCCAGGCG CTGTCGTACT TTTCGCGCCA GTATCCGTCG 
CATCCGCTGA CCGCCCAGTA CGCAGTCAAG TCGCTCTCCT CGTTCCCCGC CGAGTCCGTG 
CTGCCGTACA TTCCCCAACT GGTGCAGGCC CTGCGCCACG ACACCATGGG CTATGTGGTG 
GAGTTCATCA AGAACATCTC GAAGCGTTCG CAGATCGTCG CCCACCAGCT CATCTGGAAC 
ATGCAGACGA ATATGTATAT GGACGAGGAC CAGATGCATC GCGATCCCAA TCTGTACGAG 
GCGCTGGATC TGCTCTCGCA GAACATCATT GCCTCGTTCT CGGGCGCCGC GAAGCGATTC 
TATGAGCGGG AATTCGATTT CTTTGGCAAA ATAACAGCCG TCTCTGGCGA GATACGATCC 
TTTGCCAAGG GCATGGAGCG AAAGAATGCC TGTTTGGCGG CCCTTAGCCG CATCAAGGTG 
CAGTCGGGCT GTTATCTGCC CTCGAATCCA GAGGCCATGG TCCTGGACAT TGACTACAGC 
AGCGGCACGC CCATGCAGAG TGCGGCCAAA GCGCCGTATC TGGCCCGTTT TCGTGTCTAT 
CGGTGCGGCA TCACCGAGCT GGAGACCCGA GCCATGGAGG TGTCCAATAA TCCGAACTCC 
CAGGAGGATG CCAAGATAAC GCTGGGCGTC GAGTCGTGGC AGGCGGCCAT CTTCAAGGTG 
GGCGACGATG TGCGCCAGGA TATGCTGGCG CTTCAGGTGA TCACCATATT CAAGAATATC 
TTCCAACAAG TCGGCCTCGA TCTGTTTCTG TTTCCCTACC GCGTCGTGGC CACAGCTCCA 
GGCTGCGGCG TGATCGAGTG CGTGCCGAAT GCCAAGTCAC GCGATCAGTT GGGCAGGCAG 
ACAGACTCGG GCCTATCCGA GTACTTCCAG CATCAGTATG GCGACGAGAG CTCCAAGGAG 
TTTCAGGCGG CGCGGGCGAA CTTTGTGAAA TCGATGGCCG CCTACTCGCT GATCGGGTAT 
CTGCTGCAGA TCAAGGATCG CCACAATGGC AACATCATGA TCGACAAGGA TGGCCACATC 
ATACACATCG ATTTCGGCTT TATGTTCGAG TCATCGCCCG GTGGCAATAT CGGCTTTGAG 
CCCGACATGA AGCTCACGGA TGAGATGGTC ATGATTATGG GCGGCAAAAT GGACTCGCCC 
GCCTTCAAAT GGTTCTGCGA GCTGTGCGTG CAGGCCTTCC TGGCGGTGCG CCCCTACCAG 
GATGCGATTG TGTCTCTGGT CTCGCTGATG CTCGACACGG GGCTGCCCTG CTTCCGCGGA 
CAGACGATCA ATCTGCTCAA GCAGCGGTTC GTGGCCACCA AGAACAACAA GGAGGCCGCC 
GCCCACATGC TCTCCGTCAT ACGAAACTCT TATCAGAATT TCCGCACGCG TACCTACGAC 
ATGATCCAGT ACTATCAGAA CCAGATACCC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015041236.1
CDS	263..6718
Translation	MTMTASDKYTYQRAVLCLARVLAGIQPTPWEKVQTLFRYCPQENAAGVFCLDTRAQDAVIALGIYFLESGCQHESQIVPYLLRLAKCLPKAVWIDDARSSKIERVRIPSAEKFSFCLNTLLSDIAAKCPDSREEIILNQVETLGALANIVKSSRDSSSAPPPIILCKATVPLLFGLARSMGRYASNDPPLLCRIFPPELLPIQRVSGRDGTASSSASGTCGGSFSSSERLAATANHQFRPIIPRSMSGSLAAQYHQHFDDGRQRHLGYGFQSNSKQKPSLNSYCSVPYDPRTHFFTRYGSSFNQFPNMRVCESPTKGGPRPLYRVPPFPIQHLQTIFAVSKKLLTKDTLEHLDEQASDIFSLHQIKGYCYKSFSETLNLVLVTLLRELLQHQVDLPTPFTKDVQEFVKRLFLNGQTELQNKQQDQERERREENGITVVNKYKVNVMANAACVDLLVWAIRDETVDVDYTVPSLQNGLQFLLKKEADKLCGRLSQKLNLVLSHKIVMDHMPLLMVCLEGLGKLAQKFPNIAGTSISYLRDFLVDPSPILGKLHAHAMQTLALQKKEKELTPFKIVVQHSDSRAVVDILTDNQKHAAGGGPSGGGRSGHVAFEALRDAAIENLSIALRAAHTLDQFCVPALVANVSNRLFTAEKHESESNLVSLNIIVMLGHVAVALKDTSKTTQNILQFFIQRFCKVPSEQNALIVDQLGCMIISQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKKFHHVSDAVVNALGNIAANIQGDSEMLELLGKLLELFVQIGLDGERSYDNTPGAQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLFKDFWAYCVVMGFTNARLWPADWYQGVQQIAAKSPLLISQTAHKSDMRELNYTLAIKSDSVNELRSQILVLLEHSSDNVATAINKLTFAQCTYLLSVYWLEMLRVENADEPSLEPIMSYLCDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEIREKVLESQATLLLVYFNHIHKQIQLVADQYLSQLVDRFPHLLWNRRVLWCMLDILQLLAYSLTLDPNEETPTLRVVSTPYTLQLMDSLPARELRLKDFADRCQGIVNEAMKWAPRSTRSHLQEYPNQIPTPALAHHSGLALAIDSVVNSSYLHPGNALGTLSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEQDKAGLAERLVSDVWEACRDKSDARHRGALWRATAYLIICAEVDRKLLHAVASSQLELFTESVMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFALEQEVTHPLAAYEGCKLVSSPILIVPHLIWLQLLSEMVDTAKYCNRDKVEMFCLLLHRCLPILKSSRQNRQVATVGCRFKLLQCGLSLLQGNTIPKSLARNILRERIYSNALDYFCGPPTCPVQSRDQLLDDIMILLKFWQTMRSEKKHLVTSEVGDYDMAGHASGSSNQMLGVKANPETASLISGGADYSMRSMSASGNVTGGSSGWYNTIPHSTSTLSKRSNRSKRLQYQKDSYDKDYMKKRNLILELLAVELEFLITWYNPNSLPDLIVPGEEQITEWRNRPYKVNVWRDYARLAWCYNPSLAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALSYFSRQYPSHPLTAQYAVKSLSSFPAESVLPYIPQLVQALRHDTMGYVVEFIKNISKRSQIVAHQLIWNMQTNMYMDEDQMHRDPNLYEALDLLSQNIIASFSGAAKRFYEREFDFFGKITAVSGEIRSFAKGMERKNACLAALSRIKVQSGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRCGITELETRAMEVSNNPNSQEDAKITLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGLSEYFQHQYGDESSKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFGFMFESSPGGNIGFEPDMKLTDEMVMIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRGQTINLLKQRFVATKNNKEAAAHMLSVIRNSYQNFRTRTYDMIQYYQNQIPY

Target ORF information:

RefSeq Version	XM_015185750.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015185750.1

ORF Insert Sequence:

ATGACGATGA CCGCCTCGGA CAAGTACACG TACCAGCGGG CAGTCCTATG TCTGGCTCGA 
GTGCTGGCTG GGATCCAGCC CACACCGTGG GAGAAGGTGC AGACATTGTT CCGGTATTGC 
CCGCAGGAGA ATGCCGCGGG CGTCTTCTGT CTGGACACTA GGGCCCAGGA TGCGGTCATT 
GCGCTGGGCA TATACTTTCT GGAGAGCGGC TGCCAGCACG AGTCTCAGAT AGTGCCCTAT 
CTGCTGCGGC TGGCCAAGTG CCTGCCGAAG GCGGTCTGGA TCGATGATGC GAGGAGCAGC 
AAGATCGAAC GTGTGCGCAT CCCGTCGGCC GAAAAGTTCA GCTTCTGCCT GAACACTCTG 
CTGTCGGACA TTGCCGCCAA GTGCCCGGAC TCGCGCGAGG AGATCATACT CAATCAGGTG 
GAGACGCTGG GCGCCCTGGC CAACATCGTC AAGTCGAGCA GGGACAGCAG CTCGGCCCCG 
CCGCCGATCA TACTGTGCAA GGCCACAGTT CCGCTGCTGT TTGGACTGGC CCGTTCCATG 
GGCCGCTATG CGAGCAATGA TCCACCGCTG CTGTGCCGCA TCTTTCCGCC GGAGCTGCTG 
CCCATCCAGC GGGTAAGTGG ACGCGACGGC ACCGCCAGCA GCAGTGCCAG CGGCACCTGT 
GGCGGCTCCT TTAGCAGCAG CGAGCGGCTG GCTGCCACTG CCAACCATCA GTTTCGACCG 
ATCATACCCC GCTCCATGTC CGGCAGCCTG GCTGCACAAT ACCACCAGCA CTTTGACGAT 
GGCCGGCAGC GACACCTGGG CTATGGCTTC CAAAGCAATA GCAAGCAGAA GCCGTCCCTG 
AACAGCTATT GCTCGGTTCC GTACGACCCG CGGACGCACT TCTTCACGCG CTACGGCTCC 
AGCTTCAATC AGTTCCCCAA TATGCGGGTC TGCGAATCGC CAACAAAGGG CGGTCCTCGG 
CCCCTGTATC GAGTGCCCCC GTTTCCCATC CAGCATCTGC AGACGATATT CGCGGTGTCC 
AAGAAGCTGC TGACCAAGGA TACGCTGGAG CACCTGGACG AGCAGGCCAG CGACATATTC 
TCGCTGCACC AGATCAAGGG CTACTGCTAC AAAAGCTTCT CGGAGACGCT CAATCTGGTG 
CTGGTCACGT TGCTGCGCGA GCTGTTGCAG CATCAGGTAG ATCTGCCCAC ACCCTTCACC 
AAGGATGTCC AGGAGTTCGT CAAGCGGCTA TTCCTCAACG GCCAGACAGA GCTGCAGAAC 
AAGCAGCAGG ACCAGGAGAG GGAGCGGCGC GAGGAGAACG GCATCACCGT CGTCAACAAG 
TACAAGGTCA ATGTGATGGC CAATGCCGCC TGTGTGGATC TCCTCGTTTG GGCCATACGC 
GATGAGACGG TTGATGTCGA CTATACGGTG CCATCCCTGC AGAATGGCTT GCAATTTTTA 
TTGAAGAAAG AGGCGGACAA GCTGTGCGGC CGGCTGTCGC AGAAGCTGAA CCTGGTGCTC 
AGCCACAAGA TCGTGATGGA TCACATGCCC CTGCTGATGG TCTGCCTGGA GGGCCTGGGC 
AAGCTGGCCC AGAAGTTCCC CAACATAGCC GGCACATCGA TATCGTATCT GCGCGACTTT 
CTGGTGGACC CGAGCCCCAT TCTGGGGAAG CTGCATGCCC ATGCCATGCA GACGCTGGCC 
CTCCAGAAGA AGGAGAAGGA GCTGACGCCG TTCAAGATCG TGGTCCAGCA TTCGGACTCG 
CGTGCGGTGG TGGACATCCT GACCGATAAC CAGAAGCATG CGGCTGGTGG CGGCCCGAGC 
GGCGGCGGAC GCAGTGGCCA TGTGGCCTTC GAAGCGCTCC GGGACGCGGC CATTGAGAAC 
CTATCGATCG CCCTACGTGC TGCCCATACG CTGGATCAGT TCTGTGTCCC CGCTTTGGTG 
GCCAATGTGT CCAATCGGCT GTTTACCGCC GAGAAGCATG AGAGCGAATC GAATCTGGTG 
AGCCTGAACA TCATCGTGAT GCTGGGCCAT GTGGCCGTGG CATTGAAGGA CACCTCGAAG 
ACCACCCAGA ACATACTGCA GTTCTTCATT CAGCGCTTCT GCAAGGTGCC ATCGGAGCAG 
AACGCCCTGA TTGTCGACCA GCTGGGCTGC ATGATCATCT CCCAGTGCGA GACGCATGTC 
TTCGATGAGA TCATGAAGAT GTTCTCCCGC GTAACAGTGC AGTCGGCCTC GCTGGCGTAC 
ACCTCCGACC CGGAGCACCG GAAAAAGTTC CACCATGTCT CCGATGCGGT GGTGAATGCC 
TTGGGCAATA TAGCGGCCAA CATACAGGGC GACTCCGAGA TGCTGGAGCT GCTGGGCAAG 
CTGCTGGAGC TGTTCGTGCA GATTGGTCTG GACGGCGAGC GCTCCTACGA CAATACGCCG 
GGCGCCCAGA AGGCCAGCTC CCGGGCTGGT AATCTGGGCA TGCTGATACC GGTGATTGCG 
GTACTGGTGC GCCGTCTGCC GCCAATCAAG AATCCCCGCC AGAGGCTGCA CAAGCTCTTC 
AAGGACTTCT GGGCCTACTG TGTGGTCATG GGCTTTACCA ATGCCCGCCT CTGGCCGGCC 
GACTGGTACC AGGGTGTCCA GCAGATAGCC GCCAAATCGC CGCTCCTTAT CTCCCAAACG 
GCCCACAAGT CGGACATGCG GGAGCTCAAC TACACGCTGG CCATCAAGAG CGACTCGGTG 
AACGAGCTGC GCAGCCAGAT ACTGGTCCTG CTCGAGCACT CCTCGGACAA TGTGGCCACG 
GCCATCAACA AGCTAACGTT TGCCCAGTGC ACATACCTCC TGAGCGTCTA CTGGCTGGAG 
ATGCTGCGCG TGGAGAACGC CGACGAGCCC AGTCTGGAGC CCATCATGAG CTATCTGTGC 
GACACGGCCC TGCAGAGGGA CAAAACGGGG ATTTGGCAGT GCGTTAAATG CGTGGCCGAT 
CAGGTGTTTG AGAAGTTCCG CAATGTTCTG TACGCCCACG ATGAGATACG GGAGAAGGTG 
CTCGAGTCGC AGGCCACTCT GCTGCTCGTA TACTTCAATC ACATCCACAA GCAGATCCAA 
CTGGTGGCCG ATCAGTATCT GTCACAGCTG GTGGACCGCT TCCCCCACCT GCTGTGGAAT 
CGTCGCGTGC TCTGGTGCAT GCTGGACATC CTCCAGCTGC TGGCCTACTC GCTGACCCTC 
GATCCCAACG AGGAGACGCC CACGCTGCGA GTGGTTTCGA CGCCCTATAC CCTGCAGCTG 
ATGGACTCGC TGCCGGCGCG CGAGCTGCGG CTGAAGGACT TTGCCGATCG CTGTCAGGGC 
ATTGTCAACG AGGCCATGAA GTGGGCGCCG CGCTCGACGC GCTCCCACCT GCAGGAGTAT 
CCCAACCAGA TACCCACCCC GGCGCTGGCC CACCACAGCG GCCTGGCGCT GGCCATCGAT 
TCGGTGGTCA ACAGCAGCTA CCTCCACCCG GGCAATGCCC TGGGGACGCT CAGCAAGCGC 
CCCAGCTGCG TCAACTCGGA CACGCCCAGG TTCGTGTCGG TTCTGTGCTT GCGGAGCAAG 
TACGCGGGCG AAATCAGCGG CCTGCTGTCG GTGCTCAGTG AGCAGGACAA GGCCGGACTG 
GCCGAGCGAC TGGTCAGCGA CGTGTGGGAG GCCTGCCGGG ACAAGAGCGA TGCCCGTCAC 
CGGGGCGCCC TGTGGCGGGC CACCGCCTAC CTCATAATCT GCGCGGAGGT GGATCGCAAG 
CTGCTGCATG CCGTCGCCAG CTCCCAGCTG GAGCTCTTCA CGGAGTCCGT CATGGAGACG 
GCGGTCGAGT GCTGGCAGTG GGTGCTGACC GCACGGCAGG ACCTCGAGCT GTGCTTCATC 
CAGGAGATGG TCAGTGCCTG GCAGACGACC TTCGAGAAGC GCATGGGTCT GTTTGCCCTC 
GAGCAGGAGG TCACCCATCC ATTGGCCGCC TACGAGGGCT GCAAGCTCGT GAGCAGCCCC 
ATCCTAATTG TCCCGCACCT CATCTGGCTG CAGCTGCTCT CGGAGATGGT GGACACGGCC 
AAATACTGCA ACCGGGACAA GGTGGAGATG TTCTGCCTGC TCCTGCACCG CTGCCTGCCC 
ATTCTGAAGA GCAGCCGACA GAACCGGCAG GTGGCCACCG TCGGCTGTCG CTTCAAGCTG 
CTCCAGTGCG GCCTCTCGCT GCTGCAGGGC AACACCATAC CCAAGTCGCT GGCGCGGAAC 
ATATTGCGCG AGAGGATCTA CTCGAATGCC CTCGACTACT TCTGCGGCCC GCCCACCTGC 
CCCGTCCAGA GCAGGGACCA GCTGCTGGAC GACATAATGA TTCTGCTCAA GTTCTGGCAG 
ACGATGCGCA GCGAGAAGAA GCACTTGGTC ACCTCCGAGG TGGGCGACTA TGACATGGCG 
GGCCACGCCT CGGGCAGCTC CAACCAAATG CTGGGGGTCA AGGCCAATCC GGAGACGGCC 
TCGCTCATCA GCGGCGGGGC CGACTACTCG ATGCGATCGA TGAGCGCCTC TGGGAATGTG 
ACTGGTGGCA GCAGCGGCTG GTACAACACC ATACCCCACT CGACGTCGAC GCTGTCGAAG 
CGCTCGAATC GCTCCAAGCG GCTGCAGTAC CAGAAGGACT CCTACGACAA GGACTACATG 
AAGAAGCGCA ATCTGATACT CGAGCTGCTG GCCGTCGAGC TGGAGTTCCT CATCACCTGG 
TACAATCCCA ATAGTCTGCC CGATCTGATA GTGCCAGGCG AGGAACAGAT CACCGAGTGG 
CGCAATCGTC CGTACAAGGT GAACGTGTGG CGGGACTATG CCCGCCTGGC CTGGTGCTAC 
AATCCCTCGC TGGCCGTCTT CCTGCCGCAG CGTATCAAGA ACGCAGAGAT CATCGACGAG 
GAGGTATCCC GCCTCGTCTG TTCCGATCCG ATAGCCGTCT GTCATATACC GGAGGCCCTC 
AAGTATCTGT GCACCACCAA GAATCTGCTG CAGGAGAGCC CCGATCTGGT GTACATACTC 
TCCTGGTCGC CCGTCACCCC CATCCAGGCG CTGTCGTACT TTTCGCGCCA GTATCCGTCG 
CATCCGCTGA CCGCCCAGTA CGCAGTCAAG TCGCTCTCCT CGTTCCCCGC CGAGTCCGTG 
CTGCCGTACA TTCCCCAACT GGTGCAGGCC CTGCGCCACG ACACCATGGG CTATGTGGTG 
GAGTTCATCA AGAACATCTC GAAGCGTTCG CAGATCGTCG CCCACCAGCT CATCTGGAAC 
ATGCAGACGA ATATGTATAT GGACGAGGAC CAGATGCATC GCGATCCCAA TCTGTACGAG 
GCGCTGGATC TGCTCTCGCA GAACATCATT GCCTCGTTCT CGGGCGCCGC GAAGCGATTC 
TATGAGCGGG AATTCGATTT CTTTGGCAAA ATAACAGCCG TCTCTGGCGA GATACGATCC 
TTTGCCAAGG GCATGGAGCG AAAGAATGCC TGTTTGGCGG CCCTTAGCCG CATCAAGGTG 
CAGTCGGGCT GTTATCTGCC CTCGAATCCA GAGGCCATGG TCCTGGACAT TGACTACAGC 
AGCGGCACGC CCATGCAGAG TGCGGCCAAA GCGCCGTATC TGGCCCGTTT TCGTGTCTAT 
CGGTGCGGCA TCACCGAGCT GGAGACCCGA GCCATGGAGG TGTCCAATAA TCCGAACTCC 
CAGGAGGATG CCAAGATAAC GCTGGGCGTC GAGTCGTGGC AGGCGGCCAT CTTCAAGGTG 
GGCGACGATG TGCGCCAGGA TATGCTGGCG CTTCAGGTGA TCACCATATT CAAGAATATC 
TTCCAACAAG TCGGCCTCGA TCTGTTTCTG TTTCCCTACC GCGTCGTGGC CACAGCTCCA 
GGCTGCGGCG TGATCGAGTG CGTGCCGAAT GCCAAGTCAC GCGATCAGTT GGGCAGGCAG 
ACAGACTCGG GCCTATCCGA GTACTTCCAG CATCAGTATG GCGACGAGAG CTCCAAGGAG 
TTTCAGGCGG CGCGGGCGAA CTTTGTGAAA TCGATGGCCG CCTACTCGCT GATCGGGTAT 
CTGCTGCAGA TCAAGGATCG CCACAATGGC AACATCATGA TCGACAAGGA TGGCCACATC 
ATACACATCG ATTTCGGCTT TATGTTCGAG TCATCGCCCG GTGGCAATAT CGGCTTTGAG 
CCCGACATGA AGCTCACGGA TGAGATGGTC ATGATTATGG GCGGCAAAAT GGACTCGCCC 
GCCTTCAAAT GGTTCTGCGA GCTGTGCGTG CAGGCCTTCC TGGCGGTGCG CCCCTACCAG 
GATGCGATTG TGTCTCTGGT CTCGCTGATG CTCGACACGG GGCTGCCCTG CTTCCGCGGA 
CAGACGATCA ATCTGCTCAA GCAGCGGTTC GTGGCCACCA AGAACAACAA GGAGGCCGCC 
GCCCACATGC TCTCCGTCAT ACGAAACTCT TATCAGAATT TCCGCACGCG TACCTACGAC 
ATGATCCAGT ACTATCAGAA CCAGATACCC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA10199 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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