Dpse\thr cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\thr
Entrez Gene ID	4805385
Full Name	GA19126 gene product from transcript GA19126-RB
Synonyms	Dpse\GA19126,GA19126,dpse_GLEANR_13850,thr
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_004444439.2	XP_004444496.1	thr

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\thr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\thr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe04496	XM_004444439.2 Latest version!	Drosophila pseudoobscura pseudoobscura thr (Dpse\thr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\thr ( XM_004444439.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444496.1 Drosophila pseudoobscura pseudoobscura thr (Dpse\thr), mRNA.

Price & Availability↑

CloneID	ODe04496
Clone ID Related Accession (Same CDS sequence)	XM_004444439.2
Accession Version	XM_004444439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	thr
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444439.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAATA GAATCGTCAG TAGACTTACG GTATGTTTTG GTATATTTCT GAGGGTTGCC 
ATATTTTTCA TCCATAAGAC CGCGATAGAT CAGGTCACAC TGAAACACAC TCAAAAGAAG 
CAAAGCGTTG GTAAAAAAGT GACAATTTAT TTTAATGTAA GAATTCCGAA TCGAACGATT 
GACAAAATGT CTGCCGATAT AGTGAAACAG TTGAAAGGCA CTCGATCCGA TGTTAAGGCA 
GCCGCCACGA CAATTGAGCT GAAATTCAAG GAATTCAGCA AGGGCATCGG GATAAACGAC 
GCTTCCTTTC CGCTCAGATA TGAGTTGAGC GTTTTGCGCC AGCTGTGTCT CGCCCTGAAG 
GACAATCTGC ACCAGCATGC CGATCTCTAC TGCGGCATTG CGGCCACCAT GCTACCACAC 
GTGGAGCCCT ACGAGGAGAA GCCGAGCCTG TGGGAAGCTC ATCTAACGAG CCTGCGGTAC 
ATACATCATG GGCTTTGTCA GGAGAAATCC CTGACGGAAT GCCAGAAAAT GTACGGCCTA 
ATCCGATCGC AGCCCTGCCG CCTGCAAGAG GAGGCTGATT ACAAGTTTTA TTTGGACATC 
CATCTGACGC ACTTCAATGG CATTTACTTG CAAATGCAGA AGGAGACGTT GCCCCTCGCC 
GCCACAGATG AACTCTATTA CGCCTTGGAG GCAATGGGTG TCCTCTTTGA CACCATGCAC 
CAGCGTAAAG TGGCAAAGAA TGCAGCTCTC CTGGTCCAGC TGAATGATTC CCTGTTCAGC 
AAGCGAAGCA AAGCCTTTCT GAAGTACCTG AGCGCCCTTC CGCCCGAGAG CACGACGAAA 
ATGTACGACC CGCTTCTCAA GCTCCTGAGC TGCAGCTGGG CCACACCCAG CTCTGAGCTG 
ACTAATCAAT TCACGGAGTA TCTGGGCCTG GTTCTGGCAC TGGTGCAGAT CGATATGTTT 
TCCATAGAAG CGCCACTCGA ACAACAACTG GCACTGAAGT TGCTGCGCAT TTGTCGCGAC 
CTGTACAAGG ACGTCTCGCC GCAGAACTAC TCCATCCAGC TCCTCTACTA CTACGTCAAG 
CTGATGTACG TGCGCGAGGC TACGGCGGAC TTCAAGCAGA CTTACATCGA TCTGTGCAAG 
AAGTTCGTCT ACTTCTTCGA GCACAAGGGC GCGACGCACG CCAAAGAGCA GTGGTTCATG 
GACCTGCTGG TATTCTTCCA GCGCCTGCAG ACGCTGCTCC ATCAGAGCAG CAACAAGCCT 
CCCTTAGACA TTTTTTGGCA GCAGCTCGAA GGCGACGACA GTTCCGAGGT ATACACCGCC 
CACTTTCAAC TGCTGCATGG CTGCCTTGGG CTGGCGGTGA ACGTAGTGAG GAGTCCGCTG 
GGGACCAGCT GCTCCAACGA GGCCTGTAAA AGCATTCGCA GGCATTGCCT TCTCTACTTC 
GGGATGTGCG CCCTGGAAGC TTACATTAAC TGGCAGCCGA CGACCGAACA GAAGGCGGAC 
AAGGCACCCT ATAAGCCGCT CTTGGGCATT CTTGGCTACA CCTTGGACGT GGCCAAGAGC 
ATGAAGTGCC TGGGGCCCTC CGCCATGGAG CTAGTCAAGC TTGTGCGTCT ACTGGCACTC 
GTGGCCGAAA AGGTATCGTG TCCCGAACAA ATGTCCCTTG TGCTGGCCTT CTTGGAGCCC 
TTGCAACATC TACGACCGCT CATCGCCAGC CAGGATATGC TTAGTGTGCT CCGGAGAATC 
TACAAAGCCA GTGTCCACTG CAAGAGCTCC GACATGGCCA ACCGTCTGCA GTCCACCTAC 
CTTGCGGCCC TAACGAATCC CTCGCGGTTG CGTTCACAGC TCTTCGCCCA CTACCACAAT 
GCCAATAATA CGGAGAAGTG CGTCTACGAG TGGCACGAGT CCAGTCCTAT GCCCAACCCC 
CTAACCCCCG CCCAAACGAA ACAGCTATAC GATGTGGATC TGTTGGCCGT GCTGCACTTC 
CTGAGTGCGC CGCCCGTACC CCTGCTGCAG TCGCTACTAC GCTGCCGTCA CAATGACTAC 
CACCTGGCTC TCCTGGCACG CAAGATGCGC ACCGACAGCG AGGTGGTGCG TCAGTGCGAG 
GAACTGCGCT CCCAGCTGCA CAGCACGGCC CTCAAGCAAC CGCTCAGCCG CATGCAACAG 
CTGGCCATTG GCCATACCAG TATCAGCGTG CTGCTGGAGG CTTTGGAGGC ACAGAAGACC 
AAGTTCTCGA TCAAGGAGAC GGCCGAGAAC TGCCTGGAAG AGTTAATCGT CAAGAATAAC 
TTGCTGGAGC TCAACATTAA GCGGGAGCAT CGCCTGGTGG AGTTGGCCAC ATCGGCCATC 
GCTGGCTTCG CGGCCTTCTT CGAACGTGCA GACACAGAGC CCCTTGGCTG CGACGATACA 
CCAATCGACT GGGAAGCCCT GATCGACGAT GCAGTTGCTG CAGCCATGGC TCTGTCCACC 
ATGGGCTACA TGGCGCAGGC CGATGAAGCC TGGCTTCTGA TACTCAGGAT TGGGCAAATG 
CTGGACGAGA GATTCACCTA CCTGCGAGCC CTGACCCACT TCCTGGGACA AGACCATCTG 
AATTCTAACC AGCAGCTCCA ACTCTCCGAG GAGGTGGATC GAGCGCAGGA GCTGCTTGAC 
GATCTGTGGC CTCAGCTCCA AAACGGATGG TTCTTTAAAC GCCAACATAC TATAGTGATG 
CTCTGCCTCT GCCACATGGC CAGCTACTAT GCGCGGCAGG ACTGTCTGTG CCACGCCCAG 
CTGCTGTTGC TGCAGGCCGA GGAGCTGCGC GCCCAGTTCG ATGAGCGAGT TGGCAAGAGC 
GACATTGTGC AGATCACAAT CCAGACAGTT CGCTTTCGAT TGGAGTACCT GCGGAATAAG 
AGGTGCAGCA GCCTGCCTAG GAGGCCGACG CCCCTGCGAC AGCTAGACAC GCTGGTCGAC 
AGCGTGCGCA ACTACTGCAC AGTGTCCAGC GTGGACCTGG GAGCCCTGCA GCTGCTGCTG 
GCTGACCTCG TGCGGGAGAG CACCGAGTGT GCGGCCAACC GGCTGACCGA GCGGTTCGCC 
TTTTACGGCA CCATGCTTAA CCTGGTGCTG CAGTCGGGTA TGGCCCTGCG GACCATTGAG 
GTGCTCATCT CGTGGCTCTG GATGAACCTG CAGATGGAGT ACCTGGACCA GGCGCAGTCC 
AAGCTGCGCC TCCTAGACCA TTTGCTCGCC ATCAAGCCGC TTAGTCGAAC GCTGGTGGAG 
CAGACATCGG CCACATACGT CCCCGCAATC GCCGCCAAGG AAGATCTCAA GGCGAATGCC 
ATGAGCGAGC TGACCAGCAA CATGCTGCTC ATGCAGCTCG TGGAGCCGAT AAGGAAGCAG 
AACCAAATGG ACGTGGCCAC AATCAAATCT CTGCCCATGC ACGAGCCAAT TCCCACCAGC 
CATCAGCTGC AGCGCTATGT GAGCAAGCAA GGGACGCCGC CCCATCTACG GGACAGCATG 
CAGCTGCAAT GTATCTACTT TATCGTGGGA TGTCTCCATG CCCGCTTCAG CTTCTTGAAA 
AGGGAGAACG ATCAGCTGGA CGACTTCTAC GTGGGCGCGG GCAATTGGCT GCAGGAGGAT 
CCCGTACGCA CCGCCACACT TGGCTCCATG CTCCTCGTGC ACGAACTCTA CCACCTCAAC 
TATCTTCGCT TCAGAAAGAA GCATAAGGAG GCTCTCAGCT ATGCGGAGGC AGGTCTCAAA 
TCAGTGTACC AAACGGCGGA CATCAACTAT AGCTTCAACT TCATGGTCCA GCTGAAGACG 
GCCCGGTTGG AGCTGCATCC CGTTGGCAAA GCGAGGGCGA AGACCATTAG GCGAGCCCTG 
GCTTTCAACA CTTCGCCAGA GGATAAACGC AGGAAAGGTG TTGTTGAGGG GTCTATCAAG 
GCAAAATCAT CTGCCAGAAA GACGCCCAGG TTCAAGATAT ACACGGAGCT GGAATTGCGA 
CCCCCCATCG GCTGCAGCAA CAGCAGCAGC AGTAGCAGCA AGAGCGGCAA TGAGAACACG 
CCACCATCGG ATCATGTGGA TCTGAATGCC TGCCAGGCGA TCGAGATAAG CGATGACGAT 
GCTGCTTCGG TTTCAGCGTC GACTCCGGCT CCCTCTCAAT TGAAACGATC CCAATCGGTG 
CCAGCTAAGG CCACAAAAAC ACGCTCCGCT AGAGTTGGTA GCCAACTAAA AGTACCAGAG 
ATCATCGAAT TGGACGATAC CATGGAAGAG ACGCCTTCCA CCTCGACGGC AGCCACAGTG 
AAGCGGTATC CGACCACTGA TGCNAGGAGC TCTCGTGCAC GCAACAGGCA ACTCGAGGAA 
ACGCCAGCGA CCACGCGGGG ACGACCACGT CNGAAGGTAC CGGAGCCTGC ACCCCAGNAC 
GAGACAGTCA GCCTTAGGCC GCGACAAAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444496.1
CDS	17..4432
Translation	MYNRIVSRLTVCFGIFLRVAIFFIHKTAIDQVTLKHTQKKQSVGKKVTIYFNVRIPNRTIDKMSADIVKQLKGTRSDVKAAATTIELKFKEFSKGIGINDASFPLRYELSVLRQLCLALKDNLHQHADLYCGIAATMLPHVEPYEEKPSLWEAHLTSLRYIHHGLCQEKSLTECQKMYGLIRSQPCRLQEEADYKFYLDIHLTHFNGIYLQMQKETLPLAATDELYYALEAMGVLFDTMHQRKVAKNAALLVQLNDSLFSKRSKAFLKYLSALPPESTTKMYDPLLKLLSCSWATPSSELTNQFTEYLGLVLALVQIDMFSIEAPLEQQLALKLLRICRDLYKDVSPQNYSIQLLYYYVKLMYVREATADFKQTYIDLCKKFVYFFEHKGATHAKEQWFMDLLVFFQRLQTLLHQSSNKPPLDIFWQQLEGDDSSEVYTAHFQLLHGCLGLAVNVVRSPLGTSCSNEACKSIRRHCLLYFGMCALEAYINWQPTTEQKADKAPYKPLLGILGYTLDVAKSMKCLGPSAMELVKLVRLLALVAEKVSCPEQMSLVLAFLEPLQHLRPLIASQDMLSVLRRIYKASVHCKSSDMANRLQSTYLAALTNPSRLRSQLFAHYHNANNTEKCVYEWHESSPMPNPLTPAQTKQLYDVDLLAVLHFLSAPPVPLLQSLLRCRHNDYHLALLARKMRTDSEVVRQCEELRSQLHSTALKQPLSRMQQLAIGHTSISVLLEALEAQKTKFSIKETAENCLEELIVKNNLLELNIKREHRLVELATSAIAGFAAFFERADTEPLGCDDTPIDWEALIDDAVAAAMALSTMGYMAQADEAWLLILRIGQMLDERFTYLRALTHFLGQDHLNSNQQLQLSEEVDRAQELLDDLWPQLQNGWFFKRQHTIVMLCLCHMASYYARQDCLCHAQLLLLQAEELRAQFDERVGKSDIVQITIQTVRFRLEYLRNKRCSSLPRRPTPLRQLDTLVDSVRNYCTVSSVDLGALQLLLADLVRESTECAANRLTERFAFYGTMLNLVLQSGMALRTIEVLISWLWMNLQMEYLDQAQSKLRLLDHLLAIKPLSRTLVEQTSATYVPAIAAKEDLKANAMSELTSNMLLMQLVEPIRKQNQMDVATIKSLPMHEPIPTSHQLQRYVSKQGTPPHLRDSMQLQCIYFIVGCLHARFSFLKRENDQLDDFYVGAGNWLQEDPVRTATLGSMLLVHELYHLNYLRFRKKHKEALSYAEAGLKSVYQTADINYSFNFMVQLKTARLELHPVGKARAKTIRRALAFNTSPEDKRRKGVVEGSIKAKSSARKTPRFKIYTELELRPPIGCSNSSSSSSKSGNENTPPSDHVDLNACQAIEISDDDAASVSASTPAPSQLKRSQSVPAKATKTRSARVGSQLKVPEIIELDDTMEETPSTSTAATVKRYPTTDnullRSSRARNRQLEETPATTRGRPRnullKVPEPAPQnullETVSLRPRQRN

Target ORF information:

RefSeq Version	XM_004444439.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura thr (Dpse\thr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444439.2

ORF Insert Sequence:

ATGTACAATA GAATCGTCAG TAGACTTACG GTATGTTTTG GTATATTTCT GAGGGTTGCC 
ATATTTTTCA TCCATAAGAC CGCGATAGAT CAGGTCACAC TGAAACACAC TCAAAAGAAG 
CAAAGCGTTG GTAAAAAAGT GACAATTTAT TTTAATGTAA GAATTCCGAA TCGAACGATT 
GACAAAATGT CTGCCGATAT AGTGAAACAG TTGAAAGGCA CTCGATCCGA TGTTAAGGCA 
GCCGCCACGA CAATTGAGCT GAAATTCAAG GAATTCAGCA AGGGCATCGG GATAAACGAC 
GCTTCCTTTC CGCTCAGATA TGAGTTGAGC GTTTTGCGCC AGCTGTGTCT CGCCCTGAAG 
GACAATCTGC ACCAGCATGC CGATCTCTAC TGCGGCATTG CGGCCACCAT GCTACCACAC 
GTGGAGCCCT ACGAGGAGAA GCCGAGCCTG TGGGAAGCTC ATCTAACGAG CCTGCGGTAC 
ATACATCATG GGCTTTGTCA GGAGAAATCC CTGACGGAAT GCCAGAAAAT GTACGGCCTA 
ATCCGATCGC AGCCCTGCCG CCTGCAAGAG GAGGCTGATT ACAAGTTTTA TTTGGACATC 
CATCTGACGC ACTTCAATGG CATTTACTTG CAAATGCAGA AGGAGACGTT GCCCCTCGCC 
GCCACAGATG AACTCTATTA CGCCTTGGAG GCAATGGGTG TCCTCTTTGA CACCATGCAC 
CAGCGTAAAG TGGCAAAGAA TGCAGCTCTC CTGGTCCAGC TGAATGATTC CCTGTTCAGC 
AAGCGAAGCA AAGCCTTTCT GAAGTACCTG AGCGCCCTTC CGCCCGAGAG CACGACGAAA 
ATGTACGACC CGCTTCTCAA GCTCCTGAGC TGCAGCTGGG CCACACCCAG CTCTGAGCTG 
ACTAATCAAT TCACGGAGTA TCTGGGCCTG GTTCTGGCAC TGGTGCAGAT CGATATGTTT 
TCCATAGAAG CGCCACTCGA ACAACAACTG GCACTGAAGT TGCTGCGCAT TTGTCGCGAC 
CTGTACAAGG ACGTCTCGCC GCAGAACTAC TCCATCCAGC TCCTCTACTA CTACGTCAAG 
CTGATGTACG TGCGCGAGGC TACGGCGGAC TTCAAGCAGA CTTACATCGA TCTGTGCAAG 
AAGTTCGTCT ACTTCTTCGA GCACAAGGGC GCGACGCACG CCAAAGAGCA GTGGTTCATG 
GACCTGCTGG TATTCTTCCA GCGCCTGCAG ACGCTGCTCC ATCAGAGCAG CAACAAGCCT 
CCCTTAGACA TTTTTTGGCA GCAGCTCGAA GGCGACGACA GTTCCGAGGT ATACACCGCC 
CACTTTCAAC TGCTGCATGG CTGCCTTGGG CTGGCGGTGA ACGTAGTGAG GAGTCCGCTG 
GGGACCAGCT GCTCCAACGA GGCCTGTAAA AGCATTCGCA GGCATTGCCT TCTCTACTTC 
GGGATGTGCG CCCTGGAAGC TTACATTAAC TGGCAGCCGA CGACCGAACA GAAGGCGGAC 
AAGGCACCCT ATAAGCCGCT CTTGGGCATT CTTGGCTACA CCTTGGACGT GGCCAAGAGC 
ATGAAGTGCC TGGGGCCCTC CGCCATGGAG CTAGTCAAGC TTGTGCGTCT ACTGGCACTC 
GTGGCCGAAA AGGTATCGTG TCCCGAACAA ATGTCCCTTG TGCTGGCCTT CTTGGAGCCC 
TTGCAACATC TACGACCGCT CATCGCCAGC CAGGATATGC TTAGTGTGCT CCGGAGAATC 
TACAAAGCCA GTGTCCACTG CAAGAGCTCC GACATGGCCA ACCGTCTGCA GTCCACCTAC 
CTTGCGGCCC TAACGAATCC CTCGCGGTTG CGTTCACAGC TCTTCGCCCA CTACCACAAT 
GCCAATAATA CGGAGAAGTG CGTCTACGAG TGGCACGAGT CCAGTCCTAT GCCCAACCCC 
CTAACCCCCG CCCAAACGAA ACAGCTATAC GATGTGGATC TGTTGGCCGT GCTGCACTTC 
CTGAGTGCGC CGCCCGTACC CCTGCTGCAG TCGCTACTAC GCTGCCGTCA CAATGACTAC 
CACCTGGCTC TCCTGGCACG CAAGATGCGC ACCGACAGCG AGGTGGTGCG TCAGTGCGAG 
GAACTGCGCT CCCAGCTGCA CAGCACGGCC CTCAAGCAAC CGCTCAGCCG CATGCAACAG 
CTGGCCATTG GCCATACCAG TATCAGCGTG CTGCTGGAGG CTTTGGAGGC ACAGAAGACC 
AAGTTCTCGA TCAAGGAGAC GGCCGAGAAC TGCCTGGAAG AGTTAATCGT CAAGAATAAC 
TTGCTGGAGC TCAACATTAA GCGGGAGCAT CGCCTGGTGG AGTTGGCCAC ATCGGCCATC 
GCTGGCTTCG CGGCCTTCTT CGAACGTGCA GACACAGAGC CCCTTGGCTG CGACGATACA 
CCAATCGACT GGGAAGCCCT GATCGACGAT GCAGTTGCTG CAGCCATGGC TCTGTCCACC 
ATGGGCTACA TGGCGCAGGC CGATGAAGCC TGGCTTCTGA TACTCAGGAT TGGGCAAATG 
CTGGACGAGA GATTCACCTA CCTGCGAGCC CTGACCCACT TCCTGGGACA AGACCATCTG 
AATTCTAACC AGCAGCTCCA ACTCTCCGAG GAGGTGGATC GAGCGCAGGA GCTGCTTGAC 
GATCTGTGGC CTCAGCTCCA AAACGGATGG TTCTTTAAAC GCCAACATAC TATAGTGATG 
CTCTGCCTCT GCCACATGGC CAGCTACTAT GCGCGGCAGG ACTGTCTGTG CCACGCCCAG 
CTGCTGTTGC TGCAGGCCGA GGAGCTGCGC GCCCAGTTCG ATGAGCGAGT TGGCAAGAGC 
GACATTGTGC AGATCACAAT CCAGACAGTT CGCTTTCGAT TGGAGTACCT GCGGAATAAG 
AGGTGCAGCA GCCTGCCTAG GAGGCCGACG CCCCTGCGAC AGCTAGACAC GCTGGTCGAC 
AGCGTGCGCA ACTACTGCAC AGTGTCCAGC GTGGACCTGG GAGCCCTGCA GCTGCTGCTG 
GCTGACCTCG TGCGGGAGAG CACCGAGTGT GCGGCCAACC GGCTGACCGA GCGGTTCGCC 
TTTTACGGCA CCATGCTTAA CCTGGTGCTG CAGTCGGGTA TGGCCCTGCG GACCATTGAG 
GTGCTCATCT CGTGGCTCTG GATGAACCTG CAGATGGAGT ACCTGGACCA GGCGCAGTCC 
AAGCTGCGCC TCCTAGACCA TTTGCTCGCC ATCAAGCCGC TTAGTCGAAC GCTGGTGGAG 
CAGACATCGG CCACATACGT CCCCGCAATC GCCGCCAAGG AAGATCTCAA GGCGAATGCC 
ATGAGCGAGC TGACCAGCAA CATGCTGCTC ATGCAGCTCG TGGAGCCGAT AAGGAAGCAG 
AACCAAATGG ACGTGGCCAC AATCAAATCT CTGCCCATGC ACGAGCCAAT TCCCACCAGC 
CATCAGCTGC AGCGCTATGT GAGCAAGCAA GGGACGCCGC CCCATCTACG GGACAGCATG 
CAGCTGCAAT GTATCTACTT TATCGTGGGA TGTCTCCATG CCCGCTTCAG CTTCTTGAAA 
AGGGAGAACG ATCAGCTGGA CGACTTCTAC GTGGGCGCGG GCAATTGGCT GCAGGAGGAT 
CCCGTACGCA CCGCCACACT TGGCTCCATG CTCCTCGTGC ACGAACTCTA CCACCTCAAC 
TATCTTCGCT TCAGAAAGAA GCATAAGGAG GCTCTCAGCT ATGCGGAGGC AGGTCTCAAA 
TCAGTGTACC AAACGGCGGA CATCAACTAT AGCTTCAACT TCATGGTCCA GCTGAAGACG 
GCCCGGTTGG AGCTGCATCC CGTTGGCAAA GCGAGGGCGA AGACCATTAG GCGAGCCCTG 
GCTTTCAACA CTTCGCCAGA GGATAAACGC AGGAAAGGTG TTGTTGAGGG GTCTATCAAG 
GCAAAATCAT CTGCCAGAAA GACGCCCAGG TTCAAGATAT ACACGGAGCT GGAATTGCGA 
CCCCCCATCG GCTGCAGCAA CAGCAGCAGC AGTAGCAGCA AGAGCGGCAA TGAGAACACG 
CCACCATCGG ATCATGTGGA TCTGAATGCC TGCCAGGCGA TCGAGATAAG CGATGACGAT 
GCTGCTTCGG TTTCAGCGTC GACTCCGGCT CCCTCTCAAT TGAAACGATC CCAATCGGTG 
CCAGCTAAGG CCACAAAAAC ACGCTCCGCT AGAGTTGGTA GCCAACTAAA AGTACCAGAG 
ATCATCGAAT TGGACGATAC CATGGAAGAG ACGCCTTCCA CCTCGACGGC AGCCACAGTG 
AAGCGGTATC CGACCACTGA TGCNAGGAGC TCTCGTGCAC GCAACAGGCA ACTCGAGGAA 
ACGCCAGCGA CCACGCGGGG ACGACCACGT CNGAAGGTAC CGGAGCCTGC ACCCCAGNAC 
GAGACAGTCA GCCTTAGGCC GCGACAAAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
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Evolution of genes and genomes on the Drosophila phylogeny.
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\thr cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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