Dpse\GA21287 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA21287
Entrez Gene ID	4805001
Full Name	GA21287 gene product from transcript GA21287-RA
Synonyms	GA21287,dpse_GLEANR_14063
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001361434.3	XP_001361471.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA21287 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA21287 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe04151	XM_001361434.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21287), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA21287 ( XM_001361434.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361471.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21287), mRNA.

Price & Availability↑

CloneID	ODe04151
Clone ID Related Accession (Same CDS sequence)	XM_001361434.3
Accession Version	XM_001361434.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361434.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCAGC CTCCGACTCC AGCGCAGCAG GTGCCTGTTC TACTGCCGCC CGCTGAAGAC 
CCTCCGCCAC CTGGTGTGAA GGATCCCAGC TCATCCTCCT ACAACTACAG CCAAAACGCT 
ACGCCCCCGG TGAACGACTA CGCTCAGGGC TCTACACCTT ATTACAGGTA TCCAGACGCC 
GCCTCTTTTA ACGCTTCAGC AGCGCCAACT TTCGATCCCT ACCATCAAGG AGCGTACGGG 
TATGAGGGTT ATCAGTACCA AGGACCACCG TACGCCCAAT ACGACCAAGG CGCGTATGCA 
TACGAAGGCT ACAAATACCA AGATCGGTAC TCGTACACGC CGAACTCTCG CCCGACTAAC 
CCAAGACCCC AGTCGTACAA TGTGAATCCA GTCTACGGAA ACCACTCGGG GGAGTACAGT 
TACTCCAAGC GATATGATCC ACGACATGCG TACTACCCCC AGAGGGAACC GCGCCCATCG 
CAGCATTATT ACCCTCCAAG AGGTCCCCCA GCCGAGGAGT ATGGTCCTCG GAGCTATCGA 
GAAAGATACG AGCCCGCAGA TGGGAAAGCG CCGCAATCCA AGACAAAACC AAAAGTGGAA 
ACGGAGCGAG ATCGCCTTCT CCGCCAATGG TGCTCCAACT ATTGCGAGAA GCCAGAGGAT 
TACATTGAGA AAATGAATGC CCTCAACGAT ACGGCAGCCA CAGTCGAGTC TTGGGTACGA 
TCCTCGCCAG CCGAGCCATA TTACGAACGC ACCAATAACG AAAATGAGGT CAGGAGCAAA 
AAGCGTTTGC AGAAATTGTG CGATCTCTTT GAGGACGAAC TACTTAAGCG AGCAGATCGA 
GTGCGCAAGA CATTTCCTGT CTATGCGCCG CCTCCGAGGA AGGCGCGACG TCGCGTCTGC 
AAGCACAAAC ACAAGTCGGA GGCCTGCTCC TCGTCGTCCG ATGACGACTC GGACGAAGAC 
GGCTTCAAGG TGGAGCAGGA CTGCTGCATG GAGGAGTTGT CGCGCAAGGT GCAGCATCCG 
CAGCGTGTTC ATGCCGATCT CTGGCACAAC GATGCGGGCG AGATGAATGA CGGTCCCCTA 
TGTCGCTGCT CTGCAAAGTC GCGTCGCAGC GGTATCCGGC ATGGCATCTA TCCCGGAGAG 
ACCGGCTACA AGCTGTGCGA CAAGGCCACA AACAACGCCA ACAGTCTGTT TCATTATCGC 
ATCACCATAT CGCCGCCCAC CAACTTTCTG ACCAAGACGC CAACTATTAT CAAACACGAT 
GAGCACGAGT TTCTCTTCGA GGGATTCTCG CTGCTGTCCC ACGTGCGCCT CACCGATCTG 
CCGGTCTGCA AGGTGATTCG CTTCAATATA GAGTACACAA TTGAATACGA CGAGGAGAAG 
ATGCCCGACA ACTTCACCAT TCACGAGCTG GACCTTTTTT TCAAATACCT GTTTCACGAG 
CTGCTGGAGC TGGTTGACTT TAACCTGGAG CCCAATACTG GAGCTGATGG TGTTGAATCC 
TGCCCAGCGT TTCATTTTCT CCCCCGGTTC GTGCGTGACC TGCCCGACAA TGGCAAAGAG 
GTGCTGGCGA TGTGTGAGGT ACTCAAGTAT CTGCTGGACA ACTCGGCCGA ACTGGTGGAG 
CGGCAGCAGT TGCTTCACTT GAACCAGATC AGTCAGAGCG AATGGCAAAA CTATGTGGAC 
TTTATAAAGG GTATGCTGGT GACCAAGCCG GGCCACAAGC CATGCTCGTT GCGTGTGGAC 
CAGTTGGACA GGAACAACTC GGACTTGCCC GAGTGCGTGC ACCAGGAGTC GGGGATCTCG 
CATCCGGCCA TTGTGCACTT TGGCATACGT CCTCCCCAGT TGAGCTATGC GGGCAATCCG 
GAGTACCAGA AGGCCTGGCG TGATTACGTC AAGTTCCGGC ATTTAATGGC AAACATGTCG 
AAGCCCTCGT TCAAGGACAA ACGCAAGCTA GAGGATAAGG AGCAGCGGCT GCAGGAGATG 
CGCACTCAGG GACGGATGAA GCGCAACATC ACGGTGGCCA TCAGCTCGGA GGGTTTCTTT 
CGAACCGGCA TCATGTGCGA CATTGTGCAG CATGCCATGC TGATACCCGT GCTGACGTGC 
CATTTGCGCT TCCACAAGTC GCTGGACCTG CTCGAGGAGA GCATTGGCTA CCAATTCAAG 
AATCGCTATC TTCTGCAGCT GGCGCTGACG CATCCCTCGT ACAAGGAGAA CTACGGCACC 
AATCCGGACC ATGCTCGCAA CTCGCTCACC AACTGTGGCA TACGGCAGCC GGAGTACGGG 
GACCGCAAGA TCCACTACAT GAACACAAGG AAGCGGGGCA TCAACACGCT GGTCAGCATT 
ATGTCCCGCT TTGGCAAGGA TCAGGAGACC GTTTCGAATA TCACCCACAA CGAACGCCTG 
GAGTTCCTGG GCGACGCTGT CGTCGAATTC CTCAGTTCGA TTCATTTGTT CTTCATGTTC 
CCAGAGCTTG AGGAGGGCGG CCTGGCCACC TACCGAGCTG CCATTGTGCA AAATCAGCAT 
TTGGCTCTCT TGGCGAAGAA ATTGCAGCTA GAGGAGTTTA TGCTGTATGC CCATGGTTCC 
GATTTGTGCC ACGAACTGGA ACTGCGGCAT GCGATGGCCA ATTGCTTTGA GGCCCTGATG 
GGAGCGCTTT TGCTGGACGG CGGTATCAAA GTGGCCGACG AAGTCTTTAC GGAGGCATTG 
TTCAGGAAGG AGGGCAAACT TTTGAACATT TGGAAGAACC TGCCGGAGCA TCCGCTGCAG 
GAACAGGAGC CTTTGGGCGA TCGTCGTTGC ATTGATTCTT ATCGCGTCCT GAAGGATCTA 
ACTAAGTTCG AAGACTCGAT TGGACTTAAG TTCAAGCACA TCCGCCTTTT GGCCCGCGCC 
TTCACCGACC GCTCTATCGG TTTCACCCAC CTCACGCTGG GCTCCAATCA GCGGTTAGAG 
TTCCTGGGCG ACACTGTCCT CCAGTTGATA AGCTCGGAAT ACCTGTATCG TCACTTCCCC 
GAGCATCACG AGGGCCATTT GTCGCTTCTA CGCTCTTCGC TGGTCAATAA TCGCACTCAG 
GCCGTAGTGT GCGATGACCT GGGCATGACC AAGTATGCCG TCTACGCTAA TCCCAAGGCA 
GACCTAAAGA CCAAAGACCG CGCTGATCTG CTGGAGGCAT TTCTGGGTTC GCTATATGTC 
GACAAGGGTC TGCTGTTCTG CGAGCAGTTC TGCCATGTCT GCCTGTTTCC ACGTCTCCAG 
TTGTTTATCA TGAACCAGGA CTGGAACGAC CCAAAGTCCA AGCTGCAGCA ATGTTGCCTC 
ACACTCCGCA CTATGGACGG CGGGGAGCCG GACATACCCT ACTACAAGGT GGTCGAGGCC 
AGTGGTCCGA CCAACACGCG TGTCTACAAG GTGGCTGTCT ACTTCCGATC AAAGCGCTTA 
GCCACCTCTA GTGGTGCCTC CATTCAGCAG GCCGAGATGA ACGCAGCAAA GCAGGCCTTG 
GAGAACTCGC GGGACCTTTT CCCGCAGTTG GATCACCAGA AACGGGTGAT TGCCAAAAGC 
ATTAAGAAGC AGACGGGCAG CGAGGTGGAC AACGATGCCG ACAAACAGCA GGACGAGGAC 
AAGGCCAAGA AGCCCAAATA TACGGCGGTC CTGCAGGATG AATCCCACTT GCCCAAGCAG 
TATCGCATGC ATGAAAACAT CTCCAGCGAC GAGTTACCGG AGGATGATGA ACTTGACAAT 
GGCACAGCCA AGAGTCCGAC TGCCCATCGC TTGCCACGGA CTGGAGTCGA CACCGACAGC 
AGCAGTGACA GCGACAGCGA CAGCGACGAC TCCAAAATAA CTCCCAAGCG CCGTCGCCGC 
ATGCCATCCT CGCCATCGTC CTCATCGCCA TTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361471.2
CDS	87..3965
Translation	MYQPPTPAQQVPVLLPPAEDPPPPGVKDPSSSSYNYSQNATPPVNDYAQGSTPYYRYPDAASFNASAAPTFDPYHQGAYGYEGYQYQGPPYAQYDQGAYAYEGYKYQDRYSYTPNSRPTNPRPQSYNVNPVYGNHSGEYSYSKRYDPRHAYYPQREPRPSQHYYPPRGPPAEEYGPRSYRERYEPADGKAPQSKTKPKVETERDRLLRQWCSNYCEKPEDYIEKMNALNDTAATVESWVRSSPAEPYYERTNNENEVRSKKRLQKLCDLFEDELLKRADRVRKTFPVYAPPPRKARRRVCKHKHKSEACSSSSDDDSDEDGFKVEQDCCMEELSRKVQHPQRVHADLWHNDAGEMNDGPLCRCSAKSRRSGIRHGIYPGETGYKLCDKATNNANSLFHYRITISPPTNFLTKTPTIIKHDEHEFLFEGFSLLSHVRLTDLPVCKVIRFNIEYTIEYDEEKMPDNFTIHELDLFFKYLFHELLELVDFNLEPNTGADGVESCPAFHFLPRFVRDLPDNGKEVLAMCEVLKYLLDNSAELVERQQLLHLNQISQSEWQNYVDFIKGMLVTKPGHKPCSLRVDQLDRNNSDLPECVHQESGISHPAIVHFGIRPPQLSYAGNPEYQKAWRDYVKFRHLMANMSKPSFKDKRKLEDKEQRLQEMRTQGRMKRNITVAISSEGFFRTGIMCDIVQHAMLIPVLTCHLRFHKSLDLLEESIGYQFKNRYLLQLALTHPSYKENYGTNPDHARNSLTNCGIRQPEYGDRKIHYMNTRKRGINTLVSIMSRFGKDQETVSNITHNERLEFLGDAVVEFLSSIHLFFMFPELEEGGLATYRAAIVQNQHLALLAKKLQLEEFMLYAHGSDLCHELELRHAMANCFEALMGALLLDGGIKVADEVFTEALFRKEGKLLNIWKNLPEHPLQEQEPLGDRRCIDSYRVLKDLTKFEDSIGLKFKHIRLLARAFTDRSIGFTHLTLGSNQRLEFLGDTVLQLISSEYLYRHFPEHHEGHLSLLRSSLVNNRTQAVVCDDLGMTKYAVYANPKADLKTKDRADLLEAFLGSLYVDKGLLFCEQFCHVCLFPRLQLFIMNQDWNDPKSKLQQCCLTLRTMDGGEPDIPYYKVVEASGPTNTRVYKVAVYFRSKRLATSSGASIQQAEMNAAKQALENSRDLFPQLDHQKRVIAKSIKKQTGSEVDNDADKQQDEDKAKKPKYTAVLQDESHLPKQYRMHENISSDELPEDDELDNGTAKSPTAHRLPRTGVDTDSSSDSDSDSDDSKITPKRRRRMPSSPSSSSPLS

Target ORF information:

RefSeq Version	XM_001361434.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21287), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361434.3

ORF Insert Sequence:

ATGTATCAGC CTCCGACTCC AGCGCAGCAG GTGCCTGTTC TACTGCCGCC CGCTGAAGAC 
CCTCCGCCAC CTGGTGTGAA GGATCCCAGC TCATCCTCCT ACAACTACAG CCAAAACGCT 
ACGCCCCCGG TGAACGACTA CGCTCAGGGC TCTACACCTT ATTACAGGTA TCCAGACGCC 
GCCTCTTTTA ACGCTTCAGC AGCGCCAACT TTCGATCCCT ACCATCAAGG AGCGTACGGG 
TATGAGGGTT ATCAGTACCA AGGACCACCG TACGCCCAAT ACGACCAAGG CGCGTATGCA 
TACGAAGGCT ACAAATACCA AGATCGGTAC TCGTACACGC CGAACTCTCG CCCGACTAAC 
CCAAGACCCC AGTCGTACAA TGTGAATCCA GTCTACGGAA ACCACTCGGG GGAGTACAGT 
TACTCCAAGC GATATGATCC ACGACATGCG TACTACCCCC AGAGGGAACC GCGCCCATCG 
CAGCATTATT ACCCTCCAAG AGGTCCCCCA GCCGAGGAGT ATGGTCCTCG GAGCTATCGA 
GAAAGATACG AGCCCGCAGA TGGGAAAGCG CCGCAATCCA AGACAAAACC AAAAGTGGAA 
ACGGAGCGAG ATCGCCTTCT CCGCCAATGG TGCTCCAACT ATTGCGAGAA GCCAGAGGAT 
TACATTGAGA AAATGAATGC CCTCAACGAT ACGGCAGCCA CAGTCGAGTC TTGGGTACGA 
TCCTCGCCAG CCGAGCCATA TTACGAACGC ACCAATAACG AAAATGAGGT CAGGAGCAAA 
AAGCGTTTGC AGAAATTGTG CGATCTCTTT GAGGACGAAC TACTTAAGCG AGCAGATCGA 
GTGCGCAAGA CATTTCCTGT CTATGCGCCG CCTCCGAGGA AGGCGCGACG TCGCGTCTGC 
AAGCACAAAC ACAAGTCGGA GGCCTGCTCC TCGTCGTCCG ATGACGACTC GGACGAAGAC 
GGCTTCAAGG TGGAGCAGGA CTGCTGCATG GAGGAGTTGT CGCGCAAGGT GCAGCATCCG 
CAGCGTGTTC ATGCCGATCT CTGGCACAAC GATGCGGGCG AGATGAATGA CGGTCCCCTA 
TGTCGCTGCT CTGCAAAGTC GCGTCGCAGC GGTATCCGGC ATGGCATCTA TCCCGGAGAG 
ACCGGCTACA AGCTGTGCGA CAAGGCCACA AACAACGCCA ACAGTCTGTT TCATTATCGC 
ATCACCATAT CGCCGCCCAC CAACTTTCTG ACCAAGACGC CAACTATTAT CAAACACGAT 
GAGCACGAGT TTCTCTTCGA GGGATTCTCG CTGCTGTCCC ACGTGCGCCT CACCGATCTG 
CCGGTCTGCA AGGTGATTCG CTTCAATATA GAGTACACAA TTGAATACGA CGAGGAGAAG 
ATGCCCGACA ACTTCACCAT TCACGAGCTG GACCTTTTTT TCAAATACCT GTTTCACGAG 
CTGCTGGAGC TGGTTGACTT TAACCTGGAG CCCAATACTG GAGCTGATGG TGTTGAATCC 
TGCCCAGCGT TTCATTTTCT CCCCCGGTTC GTGCGTGACC TGCCCGACAA TGGCAAAGAG 
GTGCTGGCGA TGTGTGAGGT ACTCAAGTAT CTGCTGGACA ACTCGGCCGA ACTGGTGGAG 
CGGCAGCAGT TGCTTCACTT GAACCAGATC AGTCAGAGCG AATGGCAAAA CTATGTGGAC 
TTTATAAAGG GTATGCTGGT GACCAAGCCG GGCCACAAGC CATGCTCGTT GCGTGTGGAC 
CAGTTGGACA GGAACAACTC GGACTTGCCC GAGTGCGTGC ACCAGGAGTC GGGGATCTCG 
CATCCGGCCA TTGTGCACTT TGGCATACGT CCTCCCCAGT TGAGCTATGC GGGCAATCCG 
GAGTACCAGA AGGCCTGGCG TGATTACGTC AAGTTCCGGC ATTTAATGGC AAACATGTCG 
AAGCCCTCGT TCAAGGACAA ACGCAAGCTA GAGGATAAGG AGCAGCGGCT GCAGGAGATG 
CGCACTCAGG GACGGATGAA GCGCAACATC ACGGTGGCCA TCAGCTCGGA GGGTTTCTTT 
CGAACCGGCA TCATGTGCGA CATTGTGCAG CATGCCATGC TGATACCCGT GCTGACGTGC 
CATTTGCGCT TCCACAAGTC GCTGGACCTG CTCGAGGAGA GCATTGGCTA CCAATTCAAG 
AATCGCTATC TTCTGCAGCT GGCGCTGACG CATCCCTCGT ACAAGGAGAA CTACGGCACC 
AATCCGGACC ATGCTCGCAA CTCGCTCACC AACTGTGGCA TACGGCAGCC GGAGTACGGG 
GACCGCAAGA TCCACTACAT GAACACAAGG AAGCGGGGCA TCAACACGCT GGTCAGCATT 
ATGTCCCGCT TTGGCAAGGA TCAGGAGACC GTTTCGAATA TCACCCACAA CGAACGCCTG 
GAGTTCCTGG GCGACGCTGT CGTCGAATTC CTCAGTTCGA TTCATTTGTT CTTCATGTTC 
CCAGAGCTTG AGGAGGGCGG CCTGGCCACC TACCGAGCTG CCATTGTGCA AAATCAGCAT 
TTGGCTCTCT TGGCGAAGAA ATTGCAGCTA GAGGAGTTTA TGCTGTATGC CCATGGTTCC 
GATTTGTGCC ACGAACTGGA ACTGCGGCAT GCGATGGCCA ATTGCTTTGA GGCCCTGATG 
GGAGCGCTTT TGCTGGACGG CGGTATCAAA GTGGCCGACG AAGTCTTTAC GGAGGCATTG 
TTCAGGAAGG AGGGCAAACT TTTGAACATT TGGAAGAACC TGCCGGAGCA TCCGCTGCAG 
GAACAGGAGC CTTTGGGCGA TCGTCGTTGC ATTGATTCTT ATCGCGTCCT GAAGGATCTA 
ACTAAGTTCG AAGACTCGAT TGGACTTAAG TTCAAGCACA TCCGCCTTTT GGCCCGCGCC 
TTCACCGACC GCTCTATCGG TTTCACCCAC CTCACGCTGG GCTCCAATCA GCGGTTAGAG 
TTCCTGGGCG ACACTGTCCT CCAGTTGATA AGCTCGGAAT ACCTGTATCG TCACTTCCCC 
GAGCATCACG AGGGCCATTT GTCGCTTCTA CGCTCTTCGC TGGTCAATAA TCGCACTCAG 
GCCGTAGTGT GCGATGACCT GGGCATGACC AAGTATGCCG TCTACGCTAA TCCCAAGGCA 
GACCTAAAGA CCAAAGACCG CGCTGATCTG CTGGAGGCAT TTCTGGGTTC GCTATATGTC 
GACAAGGGTC TGCTGTTCTG CGAGCAGTTC TGCCATGTCT GCCTGTTTCC ACGTCTCCAG 
TTGTTTATCA TGAACCAGGA CTGGAACGAC CCAAAGTCCA AGCTGCAGCA ATGTTGCCTC 
ACACTCCGCA CTATGGACGG CGGGGAGCCG GACATACCCT ACTACAAGGT GGTCGAGGCC 
AGTGGTCCGA CCAACACGCG TGTCTACAAG GTGGCTGTCT ACTTCCGATC AAAGCGCTTA 
GCCACCTCTA GTGGTGCCTC CATTCAGCAG GCCGAGATGA ACGCAGCAAA GCAGGCCTTG 
GAGAACTCGC GGGACCTTTT CCCGCAGTTG GATCACCAGA AACGGGTGAT TGCCAAAAGC 
ATTAAGAAGC AGACGGGCAG CGAGGTGGAC AACGATGCCG ACAAACAGCA GGACGAGGAC 
AAGGCCAAGA AGCCCAAATA TACGGCGGTC CTGCAGGATG AATCCCACTT GCCCAAGCAG 
TATCGCATGC ATGAAAACAT CTCCAGCGAC GAGTTACCGG AGGATGATGA ACTTGACAAT 
GGCACAGCCA AGAGTCCGAC TGCCCATCGC TTGCCACGGA CTGGAGTCGA CACCGACAGC 
AGCAGTGACA GCGACAGCGA CAGCGACGAC TCCAAAATAA CTCCCAAGCG CCGTCGCCGC 
ATGCCATCCT CGCCATCGTC CTCATCGCCA TTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA21287 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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