Dpse\GA20855 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20855
Entrez Gene ID	4803355
Full Name	GA20855 gene product from transcript GA20855-RA
Synonyms	GA20855,dpse_GLEANR_14966
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001360074.3	XP_001360111.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20855 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20855 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe02742	XM_001360074.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20855), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20855 ( XM_001360074.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360111.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20855), mRNA.

Price & Availability↑

CloneID	ODe02742
Clone ID Related Accession (Same CDS sequence)	XM_001360074.3
Accession Version	XM_001360074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360074.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGGCA TTTGCCCCAG AGAGGCTGTG AGAGTTAAAG TGAAGAAATG CGAGTCGACC 
ACCAAACCAG AGTGGCGAAA GTTTTCGGTA GACCCCCAGA TTACAACTTT GGAGGTTCTG 
TACTCTTTGC TCGCCAAGGC GTTTGACGTC AAGTCGGACT TCTCCATTAA ATACAAAGCC 
TTTGATCCGG CTGGCAATGA AATTTACTTG GCCGTCCGCT CTGATTGGGA CCTCGATGCG 
GCGTTTCTTC GAATTCACAA CATTTCCATA CAGACAGCCT CAGAGCCCTG TTTGATGCTG 
CAGATCGATG TGAAACCGTT CACATTGGTA AGGGAGTGCG AGACGGAGTC ACCCGCAACC 
ACCAGCCGAT CAAGCAGCAT TACCTCATCG ACACTCTCTG CCTCCCCAAG TGGGGGGAGT 
GCTAGTGGTG GCGGTCATGC ATCTGCCCGG GAGCTGGTGT CGCCGCTGCA GTCGTTGGGA 
GTCTCGCAGA AATATGTTCA ACACATGCAG ACGAAACTGC CGGGTCTCAT CATGAATCAC 
ATGGAAAAGA CTTTTTCGAT CGTTCAGAAG GCATTCAATT TGTCCGAGGA ACACATGGCC 
AATCTTCCGC CTCGTCCTCC CATGTGCGAC AGCGAATTTC GCCTGTTCCT AGATGCTCTA 
GGCCAGATCC AGCGCAAGGA TGAGTTGCAC AAGGTGATCT TCCTGGGCGG GATCGATCCT 
AGCCTTCGCC GCGTTGTGTG GAAGCACCTG CTTAATGTCT ATCCAGGCGG GACACACGGA 
CTGGCCTTGG ATGGGCATCA GCGTATGGAG TTTATGCGGC GCAAGTCGGA GCAGTACTGC 
AGGCTTAGGG ACACATGGAA GGCGGCCATT AAACGGGGCA GTGTGGCCGG AGAACTCGCC 
TACGTAACCA GCATGGTGAA AAAGGATGTC CTGCGCACGG ACCGACTGCA TCCGTTCTAC 
GCCGGCAGCG ACGACAATCA GAACATTGCA TCGCTTTTCA ACATCCTCAC CACCTACGCC 
CTGAACCATC CCTCGGTGAG CTATTGTCAG GGAATGTCGG ATATAGCCTC GCCCCTTCTG 
GTCACCATGA ACGATGAAGC CCAGGCCTAC ATATGCTTCT GTGCCATTAT GTCTCGAGTC 
CGCGGTAACT TCATGCTAGA CGGCATCGCG ATGACGCAGA AGTTCGCACA CCTCACAGAG 
GCACTAAGCT TCTACGATCC CGAGTTCTGG GAGTACCTCA AGTCGCAGCA GGCAGACGAT 
TTACTCTTCT GTTACCGTTG GCTGCTGCTC GAGCTGAAGC GGGAGTTCCC ATTCGAGGAT 
GCGCTGCGCA TGCTGGAGGT ACAGTGGAGC TCGCTCCGTT ATGGCAGCAA TCCCAGTAGC 
GAAAAGGAGC TTCAGTTGTT TGAGAAGGAA TTCTTGGCCA TTGCGGATGC CTCAGTGCCC 
AGCAGTGCCA GCACGTTCTC CAGCTCCTAC AGCGCGACGC CGACCAGTCC TTCTTATCTA 
TTAAACAAAC CGCGAGAGAA TCCTTACACA AAGGTCTGTG CACTACGCCG TCAGAGCTCG 
TCCGCATCTC TCAGCTCGTT AAGCTCTTCC CTCGGGGCCA GCGGCCATGC TCTGGACAGC 
ACAAAGCGGT TGAATCACAG TCTGGACGAC AATATGTCTC GTCATGCGGG GGCAGCTGCT 
CGTCGTCAGC GACGAACTTC TGCCAAAGTG CACCAGAGCC TAGACGAGTC AAAAATGTTG 
GCGCTGATTG AGGGGGCCAC GGAAAGCACA ATCGTTCAGT CTGTGCAAAA ACTAACTGCC 
GGCGAGGATT CCGAGGAGGA TGATGATGTA TTTGATAGGA AGGACTCCCC GCGTTTTCTT 
GAAACGAATC CTTTTAAAGA TGATGCCCAA AAGGCCCCAG GCGCGGAAAG CCCAAAATCA 
GCCTCTCCAG GACGGGCCTT CCTGTGCAGT TCATCCTCAT CTTGCAACTT GGACGAGGCA 
CCGCAGGGCC CCGTGGGACA AAAGCCACAG CAAAATAACA TTTTATCCGT AAGCAATATA 
ATTGCAAAGC AATTGGCATC GAATGCCAGT CACGTCAGCG AGAGTGTAAA GCGCGTCAGT 
AGCGGCAGCC ACTTCAAGGA CCTCAAGGAG AAGCTTGCAT CGACGAGCCG CATTGGTATT 
CCGTTCAATG AAGATGGCGA GACTGCCATC TCCGGTAATG GCGAAAGCCA GATGCCGCCC 
AAGCTTGTCA AGAACTTCAA CGAATTTCTC AATTTCGCAA CAATTAATAA GAACCGCATC 
TCAGATCGAA TTGCCAATCA GCAGTTTTTG GGCGAAAAGC GGTCTCCCCC AGACTCTACT 
TCGCTCTCCA AGCCGCTGGT TCAGTTGACC AAAGCCGGAC TGGGAGGGTC CCTCGATGAG 
TCCGACTCTT CTTCGCTGCC AGATACTAGT TGGAGCGCCT CAACAGCGGC AACGGCCATA 
CAACAGGCGC AAGACTTGAG CAGCTACAAT CTGCAGCTGG AGTTGGAGAA CATCTCGCCA 
GGACTCCGCA CTCCGGACCT GCAACAGAGG GAACCGGGAG ACCGGGTGCC AGAGGTGAGA 
AATACCAGCG ACTGCACGCC CGACCAGGAG CCAGACCAGG ATCAAGAATA TTATCCCATG 
ACAAACGCAA TAACACGTGA GCTGCGTCTG GAGGCGGAAA ACTTGGATCG CCAAGTGTTC 
GGCCTCCCCT TCACGGAGAG CACAAAACGG CCGATGGATT CAGACGGAAT AGAGTACGAA 
GAGCTGGACA AGGACACGCT GGACATGGTA GATGATCTTA AGGAGAACGA GATCATAACG 
TGTCCCGACG TCAGCGAACT GCACATGCGA CGTCGCCAGC GACTAGCGGC CACCAAGAGC 
AACAGCGTCC AGCTGAACGA GTCCCGCACT TTCAATCCGT TCATCGACGA AAGCCAGCTG 
CTGCTGGACG GGCATAGTCC GCTGGGAATG CGCAACAGCA GTAGCCTGCC AGAGGTGATC 
CTGCCCATCT CAACAGAGCC GAATTTGGTG GAAGTATCCC CGCTTGTGGA GATTGCCACC 
AATGCCGATG ACGAGAGCGC GTACACACAT TCGCCACAAC GAACGGGCAA TCTGAATGGA 
ATCGCCGGAA CACTTGGCAC CACGTCGACG GCACTACCTC CGCCGTCAGA ATTCGGCGGT 
GGCAATGCCT TCCTGATGTT CCTTTGCCTT ACCCTGCTGC TGCAGCACCG CAACACCATC 
ATGAAAGCGG GAATGGACTA CAACGAGATT GCGATGCACT TTGACAAGAT GGTGCGCAAG 
CACGACGTTA CACGCGTTCT CAACCAAGCC AGGCGAATGT ATGTCGAGTA TTTGAAGGCC 
CAGAGCTGTT TGCAGCATCA GCAGACGACC AGTGCGACAC CGTCTCCGGG CAACCCCGTG 
TCGACGGGCA GGCAGTCGGC TACGACCTTG CCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360111.2
CDS	130..3588
Translation	MAGICPREAVRVKVKKCESTTKPEWRKFSVDPQITTLEVLYSLLAKAFDVKSDFSIKYKAFDPAGNEIYLAVRSDWDLDAAFLRIHNISIQTASEPCLMLQIDVKPFTLVRECETESPATTSRSSSITSSTLSASPSGGSASGGGHASARELVSPLQSLGVSQKYVQHMQTKLPGLIMNHMEKTFSIVQKAFNLSEEHMANLPPRPPMCDSEFRLFLDALGQIQRKDELHKVIFLGGIDPSLRRVVWKHLLNVYPGGTHGLALDGHQRMEFMRRKSEQYCRLRDTWKAAIKRGSVAGELAYVTSMVKKDVLRTDRLHPFYAGSDDNQNIASLFNILTTYALNHPSVSYCQGMSDIASPLLVTMNDEAQAYICFCAIMSRVRGNFMLDGIAMTQKFAHLTEALSFYDPEFWEYLKSQQADDLLFCYRWLLLELKREFPFEDALRMLEVQWSSLRYGSNPSSEKELQLFEKEFLAIADASVPSSASTFSSSYSATPTSPSYLLNKPRENPYTKVCALRRQSSSASLSSLSSSLGASGHALDSTKRLNHSLDDNMSRHAGAAARRQRRTSAKVHQSLDESKMLALIEGATESTIVQSVQKLTAGEDSEEDDDVFDRKDSPRFLETNPFKDDAQKAPGAESPKSASPGRAFLCSSSSSCNLDEAPQGPVGQKPQQNNILSVSNIIAKQLASNASHVSESVKRVSSGSHFKDLKEKLASTSRIGIPFNEDGETAISGNGESQMPPKLVKNFNEFLNFATINKNRISDRIANQQFLGEKRSPPDSTSLSKPLVQLTKAGLGGSLDESDSSSLPDTSWSASTAATAIQQAQDLSSYNLQLELENISPGLRTPDLQQREPGDRVPEVRNTSDCTPDQEPDQDQEYYPMTNAITRELRLEAENLDRQVFGLPFTESTKRPMDSDGIEYEELDKDTLDMVDDLKENEIITCPDVSELHMRRRQRLAATKSNSVQLNESRTFNPFIDESQLLLDGHSPLGMRNSSSLPEVILPISTEPNLVEVSPLVEIATNADDESAYTHSPQRTGNLNGIAGTLGTTSTALPPPSEFGGGNAFLMFLCLTLLLQHRNTIMKAGMDYNEIAMHFDKMVRKHDVTRVLNQARRMYVEYLKAQSCLQHQQTTSATPSPGNPVSTGRQSATTLPA

Target ORF information:

RefSeq Version	XM_001360074.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20855), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360074.3

ORF Insert Sequence:

ATGGCAGGCA TTTGCCCCAG AGAGGCTGTG AGAGTTAAAG TGAAGAAATG CGAGTCGACC 
ACCAAACCAG AGTGGCGAAA GTTTTCGGTA GACCCCCAGA TTACAACTTT GGAGGTTCTG 
TACTCTTTGC TCGCCAAGGC GTTTGACGTC AAGTCGGACT TCTCCATTAA ATACAAAGCC 
TTTGATCCGG CTGGCAATGA AATTTACTTG GCCGTCCGCT CTGATTGGGA CCTCGATGCG 
GCGTTTCTTC GAATTCACAA CATTTCCATA CAGACAGCCT CAGAGCCCTG TTTGATGCTG 
CAGATCGATG TGAAACCGTT CACATTGGTA AGGGAGTGCG AGACGGAGTC ACCCGCAACC 
ACCAGCCGAT CAAGCAGCAT TACCTCATCG ACACTCTCTG CCTCCCCAAG TGGGGGGAGT 
GCTAGTGGTG GCGGTCATGC ATCTGCCCGG GAGCTGGTGT CGCCGCTGCA GTCGTTGGGA 
GTCTCGCAGA AATATGTTCA ACACATGCAG ACGAAACTGC CGGGTCTCAT CATGAATCAC 
ATGGAAAAGA CTTTTTCGAT CGTTCAGAAG GCATTCAATT TGTCCGAGGA ACACATGGCC 
AATCTTCCGC CTCGTCCTCC CATGTGCGAC AGCGAATTTC GCCTGTTCCT AGATGCTCTA 
GGCCAGATCC AGCGCAAGGA TGAGTTGCAC AAGGTGATCT TCCTGGGCGG GATCGATCCT 
AGCCTTCGCC GCGTTGTGTG GAAGCACCTG CTTAATGTCT ATCCAGGCGG GACACACGGA 
CTGGCCTTGG ATGGGCATCA GCGTATGGAG TTTATGCGGC GCAAGTCGGA GCAGTACTGC 
AGGCTTAGGG ACACATGGAA GGCGGCCATT AAACGGGGCA GTGTGGCCGG AGAACTCGCC 
TACGTAACCA GCATGGTGAA AAAGGATGTC CTGCGCACGG ACCGACTGCA TCCGTTCTAC 
GCCGGCAGCG ACGACAATCA GAACATTGCA TCGCTTTTCA ACATCCTCAC CACCTACGCC 
CTGAACCATC CCTCGGTGAG CTATTGTCAG GGAATGTCGG ATATAGCCTC GCCCCTTCTG 
GTCACCATGA ACGATGAAGC CCAGGCCTAC ATATGCTTCT GTGCCATTAT GTCTCGAGTC 
CGCGGTAACT TCATGCTAGA CGGCATCGCG ATGACGCAGA AGTTCGCACA CCTCACAGAG 
GCACTAAGCT TCTACGATCC CGAGTTCTGG GAGTACCTCA AGTCGCAGCA GGCAGACGAT 
TTACTCTTCT GTTACCGTTG GCTGCTGCTC GAGCTGAAGC GGGAGTTCCC ATTCGAGGAT 
GCGCTGCGCA TGCTGGAGGT ACAGTGGAGC TCGCTCCGTT ATGGCAGCAA TCCCAGTAGC 
GAAAAGGAGC TTCAGTTGTT TGAGAAGGAA TTCTTGGCCA TTGCGGATGC CTCAGTGCCC 
AGCAGTGCCA GCACGTTCTC CAGCTCCTAC AGCGCGACGC CGACCAGTCC TTCTTATCTA 
TTAAACAAAC CGCGAGAGAA TCCTTACACA AAGGTCTGTG CACTACGCCG TCAGAGCTCG 
TCCGCATCTC TCAGCTCGTT AAGCTCTTCC CTCGGGGCCA GCGGCCATGC TCTGGACAGC 
ACAAAGCGGT TGAATCACAG TCTGGACGAC AATATGTCTC GTCATGCGGG GGCAGCTGCT 
CGTCGTCAGC GACGAACTTC TGCCAAAGTG CACCAGAGCC TAGACGAGTC AAAAATGTTG 
GCGCTGATTG AGGGGGCCAC GGAAAGCACA ATCGTTCAGT CTGTGCAAAA ACTAACTGCC 
GGCGAGGATT CCGAGGAGGA TGATGATGTA TTTGATAGGA AGGACTCCCC GCGTTTTCTT 
GAAACGAATC CTTTTAAAGA TGATGCCCAA AAGGCCCCAG GCGCGGAAAG CCCAAAATCA 
GCCTCTCCAG GACGGGCCTT CCTGTGCAGT TCATCCTCAT CTTGCAACTT GGACGAGGCA 
CCGCAGGGCC CCGTGGGACA AAAGCCACAG CAAAATAACA TTTTATCCGT AAGCAATATA 
ATTGCAAAGC AATTGGCATC GAATGCCAGT CACGTCAGCG AGAGTGTAAA GCGCGTCAGT 
AGCGGCAGCC ACTTCAAGGA CCTCAAGGAG AAGCTTGCAT CGACGAGCCG CATTGGTATT 
CCGTTCAATG AAGATGGCGA GACTGCCATC TCCGGTAATG GCGAAAGCCA GATGCCGCCC 
AAGCTTGTCA AGAACTTCAA CGAATTTCTC AATTTCGCAA CAATTAATAA GAACCGCATC 
TCAGATCGAA TTGCCAATCA GCAGTTTTTG GGCGAAAAGC GGTCTCCCCC AGACTCTACT 
TCGCTCTCCA AGCCGCTGGT TCAGTTGACC AAAGCCGGAC TGGGAGGGTC CCTCGATGAG 
TCCGACTCTT CTTCGCTGCC AGATACTAGT TGGAGCGCCT CAACAGCGGC AACGGCCATA 
CAACAGGCGC AAGACTTGAG CAGCTACAAT CTGCAGCTGG AGTTGGAGAA CATCTCGCCA 
GGACTCCGCA CTCCGGACCT GCAACAGAGG GAACCGGGAG ACCGGGTGCC AGAGGTGAGA 
AATACCAGCG ACTGCACGCC CGACCAGGAG CCAGACCAGG ATCAAGAATA TTATCCCATG 
ACAAACGCAA TAACACGTGA GCTGCGTCTG GAGGCGGAAA ACTTGGATCG CCAAGTGTTC 
GGCCTCCCCT TCACGGAGAG CACAAAACGG CCGATGGATT CAGACGGAAT AGAGTACGAA 
GAGCTGGACA AGGACACGCT GGACATGGTA GATGATCTTA AGGAGAACGA GATCATAACG 
TGTCCCGACG TCAGCGAACT GCACATGCGA CGTCGCCAGC GACTAGCGGC CACCAAGAGC 
AACAGCGTCC AGCTGAACGA GTCCCGCACT TTCAATCCGT TCATCGACGA AAGCCAGCTG 
CTGCTGGACG GGCATAGTCC GCTGGGAATG CGCAACAGCA GTAGCCTGCC AGAGGTGATC 
CTGCCCATCT CAACAGAGCC GAATTTGGTG GAAGTATCCC CGCTTGTGGA GATTGCCACC 
AATGCCGATG ACGAGAGCGC GTACACACAT TCGCCACAAC GAACGGGCAA TCTGAATGGA 
ATCGCCGGAA CACTTGGCAC CACGTCGACG GCACTACCTC CGCCGTCAGA ATTCGGCGGT 
GGCAATGCCT TCCTGATGTT CCTTTGCCTT ACCCTGCTGC TGCAGCACCG CAACACCATC 
ATGAAAGCGG GAATGGACTA CAACGAGATT GCGATGCACT TTGACAAGAT GGTGCGCAAG 
CACGACGTTA CACGCGTTCT CAACCAAGCC AGGCGAATGT ATGTCGAGTA TTTGAAGGCC 
CAGAGCTGTT TGCAGCATCA GCAGACGACC AGTGCGACAC CGTCTCCGGG CAACCCCGTG 
TCGACGGGCA GGCAGTCGGC TACGACCTTG CCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20855 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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