Dpse\GA13194 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA13194
Entrez Gene ID	4802373
Full Name	GA13194 gene product from transcript GA13194-RB
Synonyms	GA13194,dpse_GLEANR_5967
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001359265.4	XP_001359302.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA13194 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA13194 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe01874	XM_001359265.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13194), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA13194 ( XM_001359265.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001359302.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13194), mRNA.

Price & Availability↑

CloneID	ODe01874
Clone ID Related Accession (Same CDS sequence)	XM_001359265.4
Accession Version	XM_001359265.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001359265.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCATC AGGCGCGCTT AAGTTTGAGC ATCATGTCAC CGCATCACAG CAATCCGATT 
GCTGACTCTA CCAGAATTGA TGGGAGTCCA GTAGCTCATC GTCATTCGCC GGCGAATCTC 
ATACACAGAA CTATGAGTCT CGAACGGAGC CAAAGCACAA CTAATGGCAC CCCGGGGAAC 
CGCACCGCAT CATCGACAAC AACAATGTCG CGACAGAAAC TGAACCTCAG TCTGATGGAT 
CCCAAAACAT TTGCGGATGT GCACAGCAGT GGACTGGCCT CTCGGATTGT TGCATATCAT 
AACGACAGCA CCTTGGGCGG CCGTCGTGGC CTTCAGCGCA GCATATCTGT GAGCAATCTG 
TACAATCCAC TGAAGCAGCC AAGAAGGACC CCGTCGTCCC CACTGCCGTA CAGACCATCG 
CCACCGTCAC TGTCCATGAC TCGAATCGCG CCGCCAGAGC GCAGTTTCTA CTGCGGCAGC 
ACGCTGCCCG GCCTGCTGCG TTCCAATTCG GTGGTGGGTG TCCACAAGGT GTGCAATAAT 
GAACACGAAC AGTTATCACG GGAAAATCGC CCCATCCTGC ATCCACATCC ACCGCAGCAT 
GAGAGCGAGC CCACCAGGAG TGTTCTGGAA GAACTCAAAG AGATCTCCCG GAAGCGTATC 
AACAGTGGGG ACACACAAAA GCAGCACGAG ATTTTCAAGA GGAGCTGCCA TCGCATTGGC 
GACTTTGTGG ACCATCATCA CCATCCGCCT CCCGCGACAA CTTTCAAAAG GCAACGAGAG 
CTGAGCGTGG CAGTTCCACT GAGACACCAT CATGCGGCGA CAATGGTGCC ACCGCTGGCG 
CCGCCGACAC AAACGCAGCA TCACCCGCAG CCCACTGAGG CCGTTTCACC GGAGCAGCTG 
GCAAAGCGGC GCAACTGTAG CTACAACAAT GACATCGCCT CCTCGTTGAG TTCCAGCCGG 
CGGCACGTGA CCAAGCGCAA GCTGTACGAC ATGAGGGAAA GCATGCGGCA GACGAGCATG 
GAGGGCACTG CCAACGGCAG CTCGCCCGAG AACTCCCCCA GCGCACCAGT GTCCAAGATG 
CAGCGCAAGA CAGACGCAGA ACCCATAGCC ATAGCAAAGA GTATGCCGAT TGTAGGAGTA 
CCAGTAGTGG CGCCCTCACG CACCATCTCA GCACCGGCTA TGGGACAGCA GCACCAGATA 
AATACAGAAC TACACAGGAA CACCGAGGTG GATCTGCCAT TGGCTGCCTC CAAACCGAAA 
CTCACGCTTT TCAATACCAA GCAAACACAG CAGGTGCAAG AGCAGACCAA ACATTACGAT 
CTTGAGAGTC CCGACGTGGA TGCTGGCGAA TACGCGGGCA TAAATTTTGT GAAGCCCAAG 
CAGCAGAACT CCTTGAGTGG CGTCAAGAAT CCCAGCGTCG AGCGCACACA TAAAACGAAG 
CTGGCCATTA TGCTGAGTGG CCTGCGCGGC GAGATCTACC AGGGAGAAGA AGAAGAACCA 
GACGAAATGG ACGCCCCCGT CAGTCCAAAG ACACCCATCA AACCGATTGT TCTGACGACA 
ACCAAAGCGG TATCACCCAA CACAGCGACC AAAGTCAGCC CCTCGACAAT AACCAAGACC 
ACGACAACGT CCAGCAGTGA CAAGCCAATT ATATTGAGTG ACAAGGTGAT CGCACCAGCT 
CCAGGAACAG GAACAGCAAC AGCAACAGCC ACAGCTACCA CGACTATGAC AAATGGTTTG 
CAGCCGAAGC TTCTCTTTGG CCAAGCACCA ACACCAGCGA CAGCGACAGC GGCAGAAGGG 
GCATCACCCA ACGAGTCATC CAAAGAAACG CCAACTGCTG TCAACACAGC CCCAACAACA 
TCGAGCTCAC CTGCAGCCAC ACCAGTACTG ACATTCGGAA GTCCAAAATC CACTGCCACG 
GTTATCGGGG CCCCTTTGAA AACAACATCA GTCTTTGCCC CCACAAGCAG TGCTCAAGCG 
CCCGTACTGT CATTTGGCAA AGCGCCGCCA GTAGACACTG CAACACCATT CAAACTGGAA 
AGCAATCCCA CAAACAAGAT GAATGGAATA CCAACCAATG GGATAGCAAC TACATCGGCG 
TCTACACCTG CAGCGACTGC TAGCCCAGCA GCAATGACCT TCGGAAGCAC CATCACAAAC 
AATGCGCCAA GCACAGCCGC GTTTATGTTT GGAAAAACAG AGGTGTCCAG CGCACCAGCA 
GCAGCCAAGC CTCCTGTCTT TAGTTTTGGG GGCAGTACAG CAGCTGCACC AACACCAACA 
CCAACACAAA CACCAACAGC AACAGCAACA CCATCTCCAG CAACAGGAAC AGGAACATCT 
TTGTTTGGGA ACAGCTTTGG CAGCGCATTT AAGCAGCCCG AAGCGAACAA AGCCGCCACA 
ACAGTTCCAG AGCCAGCAAA GCCGTCAGCC ATAATCGGAG TTAAGCCGAA CTTCGGTGGC 
AACAGTTTTG GCAGCGCATT CAAGCAGCCC GCAGCCACAG TCACAGCCAC AGCCTCAACC 
ACAGCTGCGG AGCCAACAAA GGCATTCAGT TTCACGGGAA CAACGAACAC AGCGGCTGCG 
ACCACATCAA ACCTCTTTTC GTTTGGAAGT GCAGCAGTCG CAAAGCCAGC AGAACCGGCA 
CCGGCACCAG CACCAGCACC AGCGCCAGCG CCCACCTCAG GTAACATCTT TGGGCAGAGT 
GCAACTGCCA GTACACCCGC ACTGTTTTCT TTTGGTGGTG CTGCAGCAGC ATCAGCATCA 
GCAGCAGCAC CAAACACCAT CAAGGAAACA AAGCCAGCAT TTGCATTCGG AAGCGATGCC 
AGCGCTACAA CAGCTGTGCA ATCCAATGGC AGCAACAGCA GCGGCAGCAA CAATTTGTTC 
AACTTCGGTG CAGGCGTCAC GAGTGGAGCC CCTCCTTCCT TTGGCGGCGC CACATCGACC 
AATGCCCCAT CAAAAAGTGC CTTCAGCTTC AGTACAGCTC AGAAGCCGAC CTCTCAGATC 
GGCAATAATG CAGCTCCAAC AGCCGCAAAA CCATTTGCAT TTGGTGGCGG ACAGCCACAG 
GTCCCTGGAA CAACCGCATC CACCGCTGGC TCAGCTGCAT CGCCCGGAGG ATTCTCCTTT 
GTGGCCGCTG CAGGGAAGAA GGAGGCAGCA ACCAGTGGAG CGAGTCTTTT TGGAACACCG 
AACAACGCCA GCACAGGCGT TAAGCCGAGC TTTAGTTTCG GTGGAAGCAG CAACAATCAG 
CAACCCAACA ATGCGGCAAC CTCAACCCCC TCAGCAGGGC AGCAGCAGCA GTCGGCCCCG 
GGTCAGCCGG GAGCATTCTC CTTTGCCGCT GTCGCCAAGA AGGAGACCAG TGGCGCCAGC 
ATTTTTGGCC AGCCCGCCGC CGCCGCCGCC GCCACCCCCA GCGCGTCCAT CAAGCCGAGC 
TTCAGTTTTG GTGGCAGCAG CAACAACACT CAAAATGCAC CATCAGCACA TGTTGCGGCG 
CCAAGCTTTG GCGGATTTGG GGCACCTGCC GTGCCCGCTA CTAGCAATCA AAGCAAACCC 
TTTGCATTTG GTGGCACCAC AACCGCGGCG CCCAATGCCA CTCCTGCCAT AGGCGGCAAC 
TTGTTTGCCA ATGCGGTGAG CGCCGTGCAG AGCCAGGCCA AGCCGGGAGG TTTCTCGTTT 
GGCGGAACAA CAACCAAGAC CAACGCCCCG GCACCACCGG GCAACGCTCC ATTCTCGTTC 
GGAGGAGCGG CAGCTGGCGG CATGGCCTCT CCGCCCAGCA GCCAGAACGT TAACACGGCG 
AAGCCGTTCA GCTTTGGCGG CGGTGTCAGT GCTTTCGGAA GCCCCAGTCC GTCGCCGGTT 
CAGGCTGCAG CAGCCACCAA TCCAGGAGCA GGCGCCTTCA GTTTCGGTAG CCCCGCCCCA 
CCATCGGCGA GTGTGGCGCC CGGCATGTCT CAGCAAATCA ATGCCGGCAA TATGTTCGCC 
CTGCCGCCGA GCACTTCGGA GCAACGCCCC ATCCGACGAG CCACCAGGCG GCTGCAGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001359302.2
CDS	106..4128
Translation	MNHQARLSLSIMSPHHSNPIADSTRIDGSPVAHRHSPANLIHRTMSLERSQSTTNGTPGNRTASSTTTMSRQKLNLSLMDPKTFADVHSSGLASRIVAYHNDSTLGGRRGLQRSISVSNLYNPLKQPRRTPSSPLPYRPSPPSLSMTRIAPPERSFYCGSTLPGLLRSNSVVGVHKVCNNEHEQLSRENRPILHPHPPQHESEPTRSVLEELKEISRKRINSGDTQKQHEIFKRSCHRIGDFVDHHHHPPPATTFKRQRELSVAVPLRHHHAATMVPPLAPPTQTQHHPQPTEAVSPEQLAKRRNCSYNNDIASSLSSSRRHVTKRKLYDMRESMRQTSMEGTANGSSPENSPSAPVSKMQRKTDAEPIAIAKSMPIVGVPVVAPSRTISAPAMGQQHQINTELHRNTEVDLPLAASKPKLTLFNTKQTQQVQEQTKHYDLESPDVDAGEYAGINFVKPKQQNSLSGVKNPSVERTHKTKLAIMLSGLRGEIYQGEEEEPDEMDAPVSPKTPIKPIVLTTTKAVSPNTATKVSPSTITKTTTTSSSDKPIILSDKVIAPAPGTGTATATATATTTMTNGLQPKLLFGQAPTPATATAAEGASPNESSKETPTAVNTAPTTSSSPAATPVLTFGSPKSTATVIGAPLKTTSVFAPTSSAQAPVLSFGKAPPVDTATPFKLESNPTNKMNGIPTNGIATTSASTPAATASPAAMTFGSTITNNAPSTAAFMFGKTEVSSAPAAAKPPVFSFGGSTAAAPTPTPTQTPTATATPSPATGTGTSLFGNSFGSAFKQPEANKAATTVPEPAKPSAIIGVKPNFGGNSFGSAFKQPAATVTATASTTAAEPTKAFSFTGTTNTAAATTSNLFSFGSAAVAKPAEPAPAPAPAPAPAPTSGNIFGQSATASTPALFSFGGAAAASASAAAPNTIKETKPAFAFGSDASATTAVQSNGSNSSGSNNLFNFGAGVTSGAPPSFGGATSTNAPSKSAFSFSTAQKPTSQIGNNAAPTAAKPFAFGGGQPQVPGTTASTAGSAASPGGFSFVAAAGKKEAATSGASLFGTPNNASTGVKPSFSFGGSSNNQQPNNAATSTPSAGQQQQSAPGQPGAFSFAAVAKKETSGASIFGQPAAAAAATPSASIKPSFSFGGSSNNTQNAPSAHVAAPSFGGFGAPAVPATSNQSKPFAFGGTTTAAPNATPAIGGNLFANAVSAVQSQAKPGGFSFGGTTTKTNAPAPPGNAPFSFGGAAAGGMASPPSSQNVNTAKPFSFGGGVSAFGSPSPSPVQAAAATNPGAGAFSFGSPAPPSASVAPGMSQQINAGNMFALPPSTSEQRPIRRATRRLQK

Target ORF information:

RefSeq Version	XM_001359265.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13194), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001359265.4

ORF Insert Sequence:

ATGAACCATC AGGCGCGCTT AAGTTTGAGC ATCATGTCAC CGCATCACAG CAATCCGATT 
GCTGACTCTA CCAGAATTGA TGGGAGTCCA GTAGCTCATC GTCATTCGCC GGCGAATCTC 
ATACACAGAA CTATGAGTCT CGAACGGAGC CAAAGCACAA CTAATGGCAC CCCGGGGAAC 
CGCACCGCAT CATCGACAAC AACAATGTCG CGACAGAAAC TGAACCTCAG TCTGATGGAT 
CCCAAAACAT TTGCGGATGT GCACAGCAGT GGACTGGCCT CTCGGATTGT TGCATATCAT 
AACGACAGCA CCTTGGGCGG CCGTCGTGGC CTTCAGCGCA GCATATCTGT GAGCAATCTG 
TACAATCCAC TGAAGCAGCC AAGAAGGACC CCGTCGTCCC CACTGCCGTA CAGACCATCG 
CCACCGTCAC TGTCCATGAC TCGAATCGCG CCGCCAGAGC GCAGTTTCTA CTGCGGCAGC 
ACGCTGCCCG GCCTGCTGCG TTCCAATTCG GTGGTGGGTG TCCACAAGGT GTGCAATAAT 
GAACACGAAC AGTTATCACG GGAAAATCGC CCCATCCTGC ATCCACATCC ACCGCAGCAT 
GAGAGCGAGC CCACCAGGAG TGTTCTGGAA GAACTCAAAG AGATCTCCCG GAAGCGTATC 
AACAGTGGGG ACACACAAAA GCAGCACGAG ATTTTCAAGA GGAGCTGCCA TCGCATTGGC 
GACTTTGTGG ACCATCATCA CCATCCGCCT CCCGCGACAA CTTTCAAAAG GCAACGAGAG 
CTGAGCGTGG CAGTTCCACT GAGACACCAT CATGCGGCGA CAATGGTGCC ACCGCTGGCG 
CCGCCGACAC AAACGCAGCA TCACCCGCAG CCCACTGAGG CCGTTTCACC GGAGCAGCTG 
GCAAAGCGGC GCAACTGTAG CTACAACAAT GACATCGCCT CCTCGTTGAG TTCCAGCCGG 
CGGCACGTGA CCAAGCGCAA GCTGTACGAC ATGAGGGAAA GCATGCGGCA GACGAGCATG 
GAGGGCACTG CCAACGGCAG CTCGCCCGAG AACTCCCCCA GCGCACCAGT GTCCAAGATG 
CAGCGCAAGA CAGACGCAGA ACCCATAGCC ATAGCAAAGA GTATGCCGAT TGTAGGAGTA 
CCAGTAGTGG CGCCCTCACG CACCATCTCA GCACCGGCTA TGGGACAGCA GCACCAGATA 
AATACAGAAC TACACAGGAA CACCGAGGTG GATCTGCCAT TGGCTGCCTC CAAACCGAAA 
CTCACGCTTT TCAATACCAA GCAAACACAG CAGGTGCAAG AGCAGACCAA ACATTACGAT 
CTTGAGAGTC CCGACGTGGA TGCTGGCGAA TACGCGGGCA TAAATTTTGT GAAGCCCAAG 
CAGCAGAACT CCTTGAGTGG CGTCAAGAAT CCCAGCGTCG AGCGCACACA TAAAACGAAG 
CTGGCCATTA TGCTGAGTGG CCTGCGCGGC GAGATCTACC AGGGAGAAGA AGAAGAACCA 
GACGAAATGG ACGCCCCCGT CAGTCCAAAG ACACCCATCA AACCGATTGT TCTGACGACA 
ACCAAAGCGG TATCACCCAA CACAGCGACC AAAGTCAGCC CCTCGACAAT AACCAAGACC 
ACGACAACGT CCAGCAGTGA CAAGCCAATT ATATTGAGTG ACAAGGTGAT CGCACCAGCT 
CCAGGAACAG GAACAGCAAC AGCAACAGCC ACAGCTACCA CGACTATGAC AAATGGTTTG 
CAGCCGAAGC TTCTCTTTGG CCAAGCACCA ACACCAGCGA CAGCGACAGC GGCAGAAGGG 
GCATCACCCA ACGAGTCATC CAAAGAAACG CCAACTGCTG TCAACACAGC CCCAACAACA 
TCGAGCTCAC CTGCAGCCAC ACCAGTACTG ACATTCGGAA GTCCAAAATC CACTGCCACG 
GTTATCGGGG CCCCTTTGAA AACAACATCA GTCTTTGCCC CCACAAGCAG TGCTCAAGCG 
CCCGTACTGT CATTTGGCAA AGCGCCGCCA GTAGACACTG CAACACCATT CAAACTGGAA 
AGCAATCCCA CAAACAAGAT GAATGGAATA CCAACCAATG GGATAGCAAC TACATCGGCG 
TCTACACCTG CAGCGACTGC TAGCCCAGCA GCAATGACCT TCGGAAGCAC CATCACAAAC 
AATGCGCCAA GCACAGCCGC GTTTATGTTT GGAAAAACAG AGGTGTCCAG CGCACCAGCA 
GCAGCCAAGC CTCCTGTCTT TAGTTTTGGG GGCAGTACAG CAGCTGCACC AACACCAACA 
CCAACACAAA CACCAACAGC AACAGCAACA CCATCTCCAG CAACAGGAAC AGGAACATCT 
TTGTTTGGGA ACAGCTTTGG CAGCGCATTT AAGCAGCCCG AAGCGAACAA AGCCGCCACA 
ACAGTTCCAG AGCCAGCAAA GCCGTCAGCC ATAATCGGAG TTAAGCCGAA CTTCGGTGGC 
AACAGTTTTG GCAGCGCATT CAAGCAGCCC GCAGCCACAG TCACAGCCAC AGCCTCAACC 
ACAGCTGCGG AGCCAACAAA GGCATTCAGT TTCACGGGAA CAACGAACAC AGCGGCTGCG 
ACCACATCAA ACCTCTTTTC GTTTGGAAGT GCAGCAGTCG CAAAGCCAGC AGAACCGGCA 
CCGGCACCAG CACCAGCACC AGCGCCAGCG CCCACCTCAG GTAACATCTT TGGGCAGAGT 
GCAACTGCCA GTACACCCGC ACTGTTTTCT TTTGGTGGTG CTGCAGCAGC ATCAGCATCA 
GCAGCAGCAC CAAACACCAT CAAGGAAACA AAGCCAGCAT TTGCATTCGG AAGCGATGCC 
AGCGCTACAA CAGCTGTGCA ATCCAATGGC AGCAACAGCA GCGGCAGCAA CAATTTGTTC 
AACTTCGGTG CAGGCGTCAC GAGTGGAGCC CCTCCTTCCT TTGGCGGCGC CACATCGACC 
AATGCCCCAT CAAAAAGTGC CTTCAGCTTC AGTACAGCTC AGAAGCCGAC CTCTCAGATC 
GGCAATAATG CAGCTCCAAC AGCCGCAAAA CCATTTGCAT TTGGTGGCGG ACAGCCACAG 
GTCCCTGGAA CAACCGCATC CACCGCTGGC TCAGCTGCAT CGCCCGGAGG ATTCTCCTTT 
GTGGCCGCTG CAGGGAAGAA GGAGGCAGCA ACCAGTGGAG CGAGTCTTTT TGGAACACCG 
AACAACGCCA GCACAGGCGT TAAGCCGAGC TTTAGTTTCG GTGGAAGCAG CAACAATCAG 
CAACCCAACA ATGCGGCAAC CTCAACCCCC TCAGCAGGGC AGCAGCAGCA GTCGGCCCCG 
GGTCAGCCGG GAGCATTCTC CTTTGCCGCT GTCGCCAAGA AGGAGACCAG TGGCGCCAGC 
ATTTTTGGCC AGCCCGCCGC CGCCGCCGCC GCCACCCCCA GCGCGTCCAT CAAGCCGAGC 
TTCAGTTTTG GTGGCAGCAG CAACAACACT CAAAATGCAC CATCAGCACA TGTTGCGGCG 
CCAAGCTTTG GCGGATTTGG GGCACCTGCC GTGCCCGCTA CTAGCAATCA AAGCAAACCC 
TTTGCATTTG GTGGCACCAC AACCGCGGCG CCCAATGCCA CTCCTGCCAT AGGCGGCAAC 
TTGTTTGCCA ATGCGGTGAG CGCCGTGCAG AGCCAGGCCA AGCCGGGAGG TTTCTCGTTT 
GGCGGAACAA CAACCAAGAC CAACGCCCCG GCACCACCGG GCAACGCTCC ATTCTCGTTC 
GGAGGAGCGG CAGCTGGCGG CATGGCCTCT CCGCCCAGCA GCCAGAACGT TAACACGGCG 
AAGCCGTTCA GCTTTGGCGG CGGTGTCAGT GCTTTCGGAA GCCCCAGTCC GTCGCCGGTT 
CAGGCTGCAG CAGCCACCAA TCCAGGAGCA GGCGCCTTCA GTTTCGGTAG CCCCGCCCCA 
CCATCGGCGA GTGTGGCGCC CGGCATGTCT CAGCAAATCA ATGCCGGCAA TATGTTCGCC 
CTGCCGCCGA GCACTTCGGA GCAACGCCCC ATCCGACGAG CCACCAGGCG GCTGCAGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA13194 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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