Prosalpha5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prosalpha5
Entrez Gene ID	36951
Full Name	Proteasome alpha5 subunit
Synonyms	154644_at,Alpha-5,CG10938,Dmel\CG10938,PRCZ,PSA5_DROME,PSMA5,ProsMA5,alpha-5,alpha5,alpha5_dm,prosMA5
General protein information	Preferred Names Proteasome alpha5 subunit Names CG10938 gene product from transcript CG10938-RA 20s proteasome CG10938-PA CG10938-PB Prosalpha5-PA Prosalpha5-PB alpha-subunit of 20S proteasome alpha5 proteasome alpha subunit proteasome alpha5 subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54B7-54B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_057854.4	NP_477202.2	proteasome alpha5 subunit, isoform B
NM_166221.4	NP_725669.1	proteasome alpha5 subunit, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC469795	XP_525179.3
Danio rerio (zebrafish)	psma5	NP_991271.1
Saccharomyces cerevisiae (baker's yeast)	PUP2	NP_011769.1
Arabidopsis thaliana (thale cress)	PAE2	NP_188046.1
Arabidopsis thaliana (thale cress)	PAE1	NP_001077717.1
Eremothecium gossypii	AGOS_AER296W	NP_985153.1
Canis lupus familiaris (dog)	PSMA5	XP_003639184.1
Kluyveromyces lactis	KLLA0A05093g	XP_451224.1
Homo sapiens (human)	PSMA5	NP_002781.2
Bos taurus (cattle)	PSMA5	NP_001015566.1
Drosophila melanogaster (fruit fly)	Prosalpha5	NP_725669.1
Rattus norvegicus (Norway rat)	Psma5	NP_058978.1
Caenorhabditis elegans (roundworm)	pas-5	NP_492765.1
Oryza sativa (rice)	Os11g0615700	NP_001068278.1
Magnaporthe oryzae (rice blast fungus)	MGG_07130	XP_003715323.1
Macaca mulatta (Rhesus monkey)	PSMA5	XP_001090512.1
Gallus gallus (chicken)	PSMA5	NP_001026578.1
Neurospora crassa	NCU05295	XP_962066.1
Schizosaccharomyces pombe (fission yeast)	pup2	NP_594372.1
Mus musculus (house mouse)	Psma5	NP_036097.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008816	XP_314945.1
Xenopus tropicalis (tropical clawed frog)	psma5	NP_001096282.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prosalpha5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prosalpha5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13023	NM_057854.4 Latest version!	Drosophila melanogaster proteasome alpha5 subunit, transcript variant B (Prosalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa13023	NM_166221.4 Latest version!	Drosophila melanogaster proteasome alpha5 subunit, transcript variant A (Prosalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prosalpha5 ( NM_057854.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477202.2 Drosophila melanogaster proteasome alpha5 subunit, transcript variant B (Prosalpha5), mRNA.

Price & Availability↑

CloneID	OFa13023
Clone ID Related Accession (Same CDS sequence)	NM_057854.4 , NM_166221.4
Accession Version	NM_057854.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome alpha5 subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_057854.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCTCA CGCGTTCCGA GTACGACAGA GGCGTGAACA CCTTCTCGCC GGAGGGCCGC 
CTCTTCCAAG TGGAATATGC CATTGAGGCC ATCAAATTGG GCTCCACAGC AATTGGAATT 
TGCACGCCAG AAGGAGTGGT TTTGGCGGTG GAGAAGCGCA TCACTTCGCC GCTAATGGTA 
CCCAGTACTG TGGAGAAGAT TGTGGAGGTG GACAAGCACA TTGGTTGCGC CACCTCCGGC 
TTGATGGCCG ATGCCAGGAC TCTGATCGAG AGGGCACGCG TGGAGTGCCA GAACCACTGG 
TTCGTCTACA ACGAGCGCAT GTCCATCGAG TCCTGCGCCC AGGCTGTGTC CACTTTGGCC 
ATCCAGTTCG GTGATAGTGG CGACAGCGAT GGTGCCGCCG CCATGAGTCG TCCCTTTGGT 
GTGGCCATTC TATTTGCCGG CATCGAGGCG GGACAACCCC AGTTGTGGCA CATGGATCCC 
TCCGGCACAT TCGTGCGCCA CGGAGCCAAG GCCATTGGCT CGGGCAGCGA AGGTGCTCAG 
CAGAATCTGC AGGACTTATT TAGACCCGAT TTGACTCTCG ATGAGGCTAT CGACATTTCG 
CTCAACACAC TTAAACAGGT TATGGAGGAG AAACTAAACT CCACCAATGT GGAGGTGATG 
ACCATGACGA AAGAAAGAGA GTTCTACATG TTCACCAAGG AGGAGGTGGA GCAGCACATT 
AAGAACATTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477202.2
CDS	73..807
Translation	MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGICTPEGVVLAVEKRITSPLMVPSTVEKIVEVDKHIGCATSGLMADARTLIERARVECQNHWFVYNERMSIESCAQAVSTLAIQFGDSGDSDGAAAMSRPFGVAILFAGIEAGQPQLWHMDPSGTFVRHGAKAIGSGSEGAQQNLQDLFRPDLTLDEAIDISLNTLKQVMEEKLNSTNVEVMTMTKEREFYMFTKEEVEQHIKNIA

Target ORF information:

RefSeq Version	NM_057854.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome alpha5 subunit, transcript variant B (Prosalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057854.4

ORF Insert Sequence:

ATGTTTCTCA CGCGTTCCGA GTACGACAGA GGCGTGAACA CCTTCTCGCC GGAGGGCCGC 
CTCTTCCAAG TGGAATATGC CATTGAGGCC ATCAAATTGG GCTCCACAGC AATTGGAATT 
TGCACGCCAG AAGGAGTGGT TTTGGCGGTG GAGAAGCGCA TCACTTCGCC GCTAATGGTA 
CCCAGTACTG TGGAGAAGAT TGTGGAGGTG GACAAGCACA TTGGTTGCGC CACCTCCGGC 
TTGATGGCCG ATGCCAGGAC TCTGATCGAG AGGGCACGCG TGGAGTGCCA GAACCACTGG 
TTCGTCTACA ACGAGCGCAT GTCCATCGAG TCCTGCGCCC AGGCTGTGTC CACTTTGGCC 
ATCCAGTTCG GTGATAGTGG CGACAGCGAT GGTGCCGCCG CCATGAGTCG TCCCTTTGGT 
GTGGCCATTC TATTTGCCGG CATCGAGGCG GGACAACCCC AGTTGTGGCA CATGGATCCC 
TCCGGCACAT TCGTGCGCCA CGGAGCCAAG GCCATTGGCT CGGGCAGCGA AGGTGCTCAG 
CAGAATCTGC AGGACTTATT TAGACCCGAT TTGACTCTCG ATGAGGCTAT CGACATTTCG 
CTCAACACAC TTAAACAGGT TATGGAGGAG AAACTAAACT CCACCAATGT GGAGGTGATG 
ACCATGACGA AAGAAAGAGA GTTCTACATG TTCACCAAGG AGGAGGTGGA GCAGCACATT 
AAGAACATTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prosalpha5 ( NM_166221.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725669.1 Drosophila melanogaster proteasome alpha5 subunit, transcript variant A (Prosalpha5), mRNA.

Price & Availability↑

CloneID	OFa13023
Clone ID Related Accession (Same CDS sequence)	NM_057854.4 , NM_166221.4
Accession Version	NM_166221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome alpha5 subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166221.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCTCA CGCGTTCCGA GTACGACAGA GGCGTGAACA CCTTCTCGCC GGAGGGCCGC 
CTCTTCCAAG TGGAATATGC CATTGAGGCC ATCAAATTGG GCTCCACAGC AATTGGAATT 
TGCACGCCAG AAGGAGTGGT TTTGGCGGTG GAGAAGCGCA TCACTTCGCC GCTAATGGTA 
CCCAGTACTG TGGAGAAGAT TGTGGAGGTG GACAAGCACA TTGGTTGCGC CACCTCCGGC 
TTGATGGCCG ATGCCAGGAC TCTGATCGAG AGGGCACGCG TGGAGTGCCA GAACCACTGG 
TTCGTCTACA ACGAGCGCAT GTCCATCGAG TCCTGCGCCC AGGCTGTGTC CACTTTGGCC 
ATCCAGTTCG GTGATAGTGG CGACAGCGAT GGTGCCGCCG CCATGAGTCG TCCCTTTGGT 
GTGGCCATTC TATTTGCCGG CATCGAGGCG GGACAACCCC AGTTGTGGCA CATGGATCCC 
TCCGGCACAT TCGTGCGCCA CGGAGCCAAG GCCATTGGCT CGGGCAGCGA AGGTGCTCAG 
CAGAATCTGC AGGACTTATT TAGACCCGAT TTGACTCTCG ATGAGGCTAT CGACATTTCG 
CTCAACACAC TTAAACAGGT TATGGAGGAG AAACTAAACT CCACCAATGT GGAGGTGATG 
ACCATGACGA AAGAAAGAGA GTTCTACATG TTCACCAAGG AGGAGGTGGA GCAGCACATT 
AAGAACATTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725669.1
CDS	84..818
Translation	MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGICTPEGVVLAVEKRITSPLMVPSTVEKIVEVDKHIGCATSGLMADARTLIERARVECQNHWFVYNERMSIESCAQAVSTLAIQFGDSGDSDGAAAMSRPFGVAILFAGIEAGQPQLWHMDPSGTFVRHGAKAIGSGSEGAQQNLQDLFRPDLTLDEAIDISLNTLKQVMEEKLNSTNVEVMTMTKEREFYMFTKEEVEQHIKNIA

Target ORF information:

RefSeq Version	NM_166221.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome alpha5 subunit, transcript variant A (Prosalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166221.4

ORF Insert Sequence:

ATGTTTCTCA CGCGTTCCGA GTACGACAGA GGCGTGAACA CCTTCTCGCC GGAGGGCCGC 
CTCTTCCAAG TGGAATATGC CATTGAGGCC ATCAAATTGG GCTCCACAGC AATTGGAATT 
TGCACGCCAG AAGGAGTGGT TTTGGCGGTG GAGAAGCGCA TCACTTCGCC GCTAATGGTA 
CCCAGTACTG TGGAGAAGAT TGTGGAGGTG GACAAGCACA TTGGTTGCGC CACCTCCGGC 
TTGATGGCCG ATGCCAGGAC TCTGATCGAG AGGGCACGCG TGGAGTGCCA GAACCACTGG 
TTCGTCTACA ACGAGCGCAT GTCCATCGAG TCCTGCGCCC AGGCTGTGTC CACTTTGGCC 
ATCCAGTTCG GTGATAGTGG CGACAGCGAT GGTGCCGCCG CCATGAGTCG TCCCTTTGGT 
GTGGCCATTC TATTTGCCGG CATCGAGGCG GGACAACCCC AGTTGTGGCA CATGGATCCC 
TCCGGCACAT TCGTGCGCCA CGGAGCCAAG GCCATTGGCT CGGGCAGCGA AGGTGCTCAG 
CAGAATCTGC AGGACTTATT TAGACCCGAT TTGACTCTCG ATGAGGCTAT CGACATTTCG 
CTCAACACAC TTAAACAGGT TATGGAGGAG AAACTAAACT CCACCAATGT GGAGGTGATG 
ACCATGACGA AAGAAAGAGA GTTCTACATG TTCACCAAGG AGGAGGTGGA GCAGCACATT 
AAGAACATTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Prosalpha5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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