LOC6547749 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6547749
Entrez Gene ID	6547749
Full Name	GG22771 gene product from transcript GG22771-RT
Synonyms	GG22771,dere_GLEANR_7470
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_015157116.1	XP_015012602.1	uncharacterized protein, isoform AC
XM_015157123.1	XP_015012609.1	uncharacterized protein, isoform E
XM_015157100.1	XP_015012586.1	uncharacterized protein, isoform M
XM_015157105.2	XP_015012591.1	myosin heavy chain, muscle isoform X6
XM_015157114.1	XP_015012600.1	uncharacterized protein, isoform AA
XM_015157122.1	XP_015012608.1	uncharacterized protein, isoform C
XM_015157125.1	XP_015012611.1	uncharacterized protein, isoform G
XM_015157128.1	XP_015012614.1	uncharacterized protein, isoform J
XM_015157130.1	XP_015012616.1	uncharacterized protein, isoform L
XM_001976222.2	XP_001976258.2	uncharacterized protein, isoform D
XM_026979932.1	XP_026835733.1	myosin heavy chain, muscle isoform X3
XM_026979936.1	XP_026835737.1	myosin heavy chain, muscle isoform X1
XM_015157111.1	XP_015012597.1	uncharacterized protein, isoform X
XM_015157113.1	XP_015012599.1	uncharacterized protein, isoform Z
XM_015157115.1	XP_015012601.1	uncharacterized protein, isoform AB
XM_015157119.2	XP_015012605.1	myosin heavy chain, muscle isoform X11
XM_015157120.1	XP_015012606.1	uncharacterized protein, isoform AG
XM_015157120.2	XP_015012606.1	myosin heavy chain, muscle isoform X13
XM_015157124.1	XP_015012610.1	uncharacterized protein, isoform F
XM_015157129.1	XP_015012615.1	uncharacterized protein, isoform K
XM_026979933.1	XP_026835734.1	myosin heavy chain, muscle isoform X4
XM_015157102.1	XP_015012588.1	uncharacterized protein, isoform O
XM_015157103.1	XP_015012589.1	uncharacterized protein, isoform P
XM_015157103.2	XP_015012589.1	myosin heavy chain, muscle isoform X12
XM_015157104.1	XP_015012590.1	uncharacterized protein, isoform Q
XM_015157107.1	XP_015012593.1	uncharacterized protein, isoform T
XM_015157108.1	XP_015012594.1	uncharacterized protein, isoform U
XM_015157109.1	XP_015012595.1	uncharacterized protein, isoform V
XM_015157110.1	XP_015012596.1	uncharacterized protein, isoform W
XM_015157117.1	XP_015012603.1	uncharacterized protein, isoform AD
XM_015157121.1	XP_015012607.1	uncharacterized protein, isoform B
XM_015157100.2	XP_015012586.1	myosin heavy chain, muscle isoform X10
XM_015157101.2	XP_015012587.1	myosin heavy chain, muscle isoform X2
XM_015157112.1	XP_015012598.1	uncharacterized protein, isoform Y
XM_015157113.2	XP_015012599.1	myosin heavy chain, muscle isoform X5
XM_015157118.1	XP_015012604.1	uncharacterized protein, isoform AE
XM_015157119.1	XP_015012605.1	uncharacterized protein, isoform AF
XM_015157125.2	XP_015012611.1	myosin heavy chain, muscle isoform X8
XM_015157126.1	XP_015012612.1	uncharacterized protein, isoform H
XM_015157101.1	XP_015012587.1	uncharacterized protein, isoform N
XM_015157105.1	XP_015012591.1	uncharacterized protein, isoform R
XM_015157106.1	XP_015012592.1	uncharacterized protein, isoform S
XM_015157112.2	XP_015012598.1	myosin heavy chain, muscle isoform X1
XM_015157122.2	XP_015012608.1	myosin heavy chain, muscle isoform X9
XM_015157127.1	XP_015012613.1	uncharacterized protein, isoform I
XM_026979934.1	XP_026835735.1	myosin heavy chain, muscle isoform X7

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6547749 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6547749 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi219397	XM_026979932.1 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi219398	XM_026979934.1 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi35946	XM_001976222.2 Latest version!	Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36874	XM_015157100.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36875	XM_015157101.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36876	XM_015157102.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant O (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36877	XM_015157103.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36878	XM_015157104.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant Q (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36879	XM_015157105.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36880	XM_015157106.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant S (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36881	XM_015157107.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant T (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36882	XM_015157108.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant U (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36883	XM_015157109.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant V (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36884	XM_015157110.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant W (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36885	XM_015157111.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant X (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36886	XM_015157112.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36886	XM_026979936.1 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36886	XM_026979933.1 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36887	XM_015157113.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36888	XM_015157114.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant AA (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36889	XM_015157115.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant AB (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36890	XM_015157116.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant AC (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36891	XM_015157117.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant AD (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36892	XM_015157118.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant AE (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36893	XM_015157119.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36894	XM_015157120.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36895	XM_015157121.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36896	XM_015157122.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36897	XM_015157123.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36898	XM_015157124.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36899	XM_015157125.2 Latest version!	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36900	XM_015157126.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36901	XM_015157127.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36902	XM_015157128.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36903	XM_015157129.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36904	XM_015157130.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant L (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODi36894	XM_015157120.1	Drosophila erecta uncharacterized protein, transcript variant AG (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36877	XM_015157103.1	Drosophila erecta uncharacterized protein, transcript variant P (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36874	XM_015157100.1	Drosophila erecta uncharacterized protein, transcript variant M (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36887	XM_015157113.1	Drosophila erecta uncharacterized protein, transcript variant Z (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36893	XM_015157119.1	Drosophila erecta uncharacterized protein, transcript variant AF (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36899	XM_015157125.1	Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36875	XM_015157101.1	Drosophila erecta uncharacterized protein, transcript variant N (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36879	XM_015157105.1	Drosophila erecta uncharacterized protein, transcript variant R (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36886	XM_015157112.1	Drosophila erecta uncharacterized protein, transcript variant Y (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi36896	XM_015157122.1	Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG22771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6547749 ( XM_026979932.1 ) cDNA ORF clone, Drosophila erecta -> XP_026835733.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODi219397
Clone ID Related Accession (Same CDS sequence)	XM_026979932.1
Accession Version	XM_026979932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026835733.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_026979932.1
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026979932.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_026979934.1 ) cDNA ORF clone, Drosophila erecta -> XP_026835735.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X7, mRNA.

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CloneID	ODi219398
Clone ID Related Accession (Same CDS sequence)	XM_026979934.1
Accession Version	XM_026979934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026835735.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_026979934.1
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026979934.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_001976222.2 ) cDNA ORF clone, Drosophila erecta -> XP_001976258.2 Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG22771), mRNA.

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CloneID	ODi35946
Clone ID Related Accession (Same CDS sequence)	XM_001976222.2
Accession Version	XM_001976222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). On Dec 21, 2015 this sequence version replaced XM_001976222.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001976258.2
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_001976222.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001976222.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157100.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012586.1 Drosophila erecta uncharacterized protein, transcript variant M (Dere\GG22771), mRNA.

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CloneID	ODi36874
Clone ID Related Accession (Same CDS sequence)	XM_015157100.1 , XM_015157100.2
Accession Version	XM_015157100.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform M
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012586.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157100.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant M (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157100.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157100.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012586.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X10, mRNA.

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CloneID	ODi36874
Clone ID Related Accession (Same CDS sequence)	XM_015157100.1 , XM_015157100.2
Accession Version	XM_015157100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012586.1
CDS	284..6172
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157100.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157100.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157101.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012587.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X2, mRNA.

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CloneID	ODi36875
Clone ID Related Accession (Same CDS sequence)	XM_015157101.2 , XM_015157101.1
Accession Version	XM_015157101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012587.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157101.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157101.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157101.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012587.1 Drosophila erecta uncharacterized protein, transcript variant N (Dere\GG22771), mRNA.

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CloneID	ODi36875
Clone ID Related Accession (Same CDS sequence)	XM_015157101.2 , XM_015157101.1
Accession Version	XM_015157101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform N
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012587.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157101.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant N (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157101.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157102.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012588.1 Drosophila erecta uncharacterized protein, transcript variant O (Dere\GG22771), mRNA.

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CloneID	ODi36876
Clone ID Related Accession (Same CDS sequence)	XM_015157102.1
Accession Version	XM_015157102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform O
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012588.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157102.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant O (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157102.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157103.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012589.1 Drosophila erecta uncharacterized protein, transcript variant P (Dere\GG22771), mRNA.

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CloneID	ODi36877
Clone ID Related Accession (Same CDS sequence)	XM_015157103.1 , XM_015157103.2
Accession Version	XM_015157103.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform P
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012589.1
CDS	121..5970
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157103.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant P (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157103.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157103.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012589.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X13, mRNA.

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CloneID	ODi36877
Clone ID Related Accession (Same CDS sequence)	XM_015157103.1 , XM_015157103.2
Accession Version	XM_015157103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012589.1
CDS	195..6044
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157103.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157103.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157104.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012590.1 Drosophila erecta uncharacterized protein, transcript variant Q (Dere\GG22771), mRNA.

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CloneID	ODi36878
Clone ID Related Accession (Same CDS sequence)	XM_015157104.1
Accession Version	XM_015157104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform Q
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012590.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157104.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant Q (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157104.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157105.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012591.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X6, mRNA.

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CloneID	ODi36879
Clone ID Related Accession (Same CDS sequence)	XM_015157105.2 , XM_015157105.1
Accession Version	XM_015157105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012591.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157105.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157105.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157105.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012591.1 Drosophila erecta uncharacterized protein, transcript variant R (Dere\GG22771), mRNA.

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CloneID	ODi36879
Clone ID Related Accession (Same CDS sequence)	XM_015157105.2 , XM_015157105.1
Accession Version	XM_015157105.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform R
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012591.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157105.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant R (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157105.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157106.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012592.1 Drosophila erecta uncharacterized protein, transcript variant S (Dere\GG22771), mRNA.

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CloneID	ODi36880
Clone ID Related Accession (Same CDS sequence)	XM_015157106.1
Accession Version	XM_015157106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform S
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012592.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157106.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant S (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157106.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157107.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012593.1 Drosophila erecta uncharacterized protein, transcript variant T (Dere\GG22771), mRNA.

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CloneID	ODi36881
Clone ID Related Accession (Same CDS sequence)	XM_015157107.1
Accession Version	XM_015157107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform T
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012593.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157107.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant T (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157107.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157108.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012594.1 Drosophila erecta uncharacterized protein, transcript variant U (Dere\GG22771), mRNA.

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CloneID	ODi36882
Clone ID Related Accession (Same CDS sequence)	XM_015157108.1
Accession Version	XM_015157108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform U
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012594.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157108.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant U (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157108.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157109.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012595.1 Drosophila erecta uncharacterized protein, transcript variant V (Dere\GG22771), mRNA.

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CloneID	ODi36883
Clone ID Related Accession (Same CDS sequence)	XM_015157109.1
Accession Version	XM_015157109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform V
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012595.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157109.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant V (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157109.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157110.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012596.1 Drosophila erecta uncharacterized protein, transcript variant W (Dere\GG22771), mRNA.

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CloneID	ODi36884
Clone ID Related Accession (Same CDS sequence)	XM_015157110.1
Accession Version	XM_015157110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform W
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012596.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157110.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant W (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157110.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157111.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012597.1 Drosophila erecta uncharacterized protein, transcript variant X (Dere\GG22771), mRNA.

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CloneID	ODi36885
Clone ID Related Accession (Same CDS sequence)	XM_015157111.1
Accession Version	XM_015157111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform X
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012597.1
CDS	98..5908
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAI

Target ORF information:

RefSeq Version	XM_015157111.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant X (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157111.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_026979936.1 ) cDNA ORF clone, Drosophila erecta -> XP_026835737.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X12, mRNA.

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CloneID	ODi36886
Clone ID Related Accession (Same CDS sequence)	XM_026979936.1 , XM_026979933.1 , XM_015157112.1 , XM_015157112.2
Accession Version	XM_026979936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026835737.1
CDS	202..6090
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_026979936.1
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026979936.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_026979933.1 ) cDNA ORF clone, Drosophila erecta -> XP_026835734.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X4, mRNA.

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CloneID	ODi36886
Clone ID Related Accession (Same CDS sequence)	XM_026979936.1 , XM_026979933.1 , XM_015157112.1 , XM_015157112.2
Accession Version	XM_026979933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026835734.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_026979933.1
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026979933.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157112.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012598.1 Drosophila erecta uncharacterized protein, transcript variant Y (Dere\GG22771), mRNA.

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CloneID	ODi36886
Clone ID Related Accession (Same CDS sequence)	XM_026979936.1 , XM_026979933.1 , XM_015157112.1 , XM_015157112.2
Accession Version	XM_015157112.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform Y
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012598.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157112.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant Y (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157112.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157112.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012598.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X1, mRNA.

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CloneID	ODi36886
Clone ID Related Accession (Same CDS sequence)	XM_026979936.1 , XM_026979933.1 , XM_015157112.1 , XM_015157112.2
Accession Version	XM_015157112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012598.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157112.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157112.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157113.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012599.1 Drosophila erecta uncharacterized protein, transcript variant Z (Dere\GG22771), mRNA.

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CloneID	ODi36887
Clone ID Related Accession (Same CDS sequence)	XM_015157113.1 , XM_015157113.2
Accession Version	XM_015157113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform Z
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012599.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157113.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant Z (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157113.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157113.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012599.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X5, mRNA.

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CloneID	ODi36887
Clone ID Related Accession (Same CDS sequence)	XM_015157113.1 , XM_015157113.2
Accession Version	XM_015157113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012599.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157113.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157113.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157114.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012600.1 Drosophila erecta uncharacterized protein, transcript variant AA (Dere\GG22771), mRNA.

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CloneID	ODi36888
Clone ID Related Accession (Same CDS sequence)	XM_015157114.1
Accession Version	XM_015157114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012600.1
CDS	98..5947
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157114.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AA (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157114.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157115.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012601.1 Drosophila erecta uncharacterized protein, transcript variant AB (Dere\GG22771), mRNA.

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CloneID	ODi36889
Clone ID Related Accession (Same CDS sequence)	XM_015157115.1
Accession Version	XM_015157115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012601.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157115.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AB (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157115.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157116.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012602.1 Drosophila erecta uncharacterized protein, transcript variant AC (Dere\GG22771), mRNA.

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CloneID	ODi36890
Clone ID Related Accession (Same CDS sequence)	XM_015157116.1
Accession Version	XM_015157116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AC
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012602.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157116.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AC (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157116.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157117.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012603.1 Drosophila erecta uncharacterized protein, transcript variant AD (Dere\GG22771), mRNA.

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CloneID	ODi36891
Clone ID Related Accession (Same CDS sequence)	XM_015157117.1
Accession Version	XM_015157117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AD
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012603.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157117.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AD (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157117.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157118.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012604.1 Drosophila erecta uncharacterized protein, transcript variant AE (Dere\GG22771), mRNA.

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CloneID	ODi36892
Clone ID Related Accession (Same CDS sequence)	XM_015157118.1
Accession Version	XM_015157118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AE
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012604.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157118.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AE (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157118.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157119.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012605.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X11, mRNA.

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CloneID	ODi36893
Clone ID Related Accession (Same CDS sequence)	XM_015157119.2 , XM_015157119.1
Accession Version	XM_015157119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012605.1
CDS	284..6172
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157119.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157119.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157119.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012605.1 Drosophila erecta uncharacterized protein, transcript variant AF (Dere\GG22771), mRNA.

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CloneID	ODi36893
Clone ID Related Accession (Same CDS sequence)	XM_015157119.2 , XM_015157119.1
Accession Version	XM_015157119.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AF
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012605.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157119.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AF (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157119.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157120.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012606.1 Drosophila erecta uncharacterized protein, transcript variant AG (Dere\GG22771), mRNA.

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CloneID	ODi36894
Clone ID Related Accession (Same CDS sequence)	XM_015157120.1 , XM_015157120.2
Accession Version	XM_015157120.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform AG
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012606.1
CDS	119..5968
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157120.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant AG (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157120.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157120.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012606.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X14, mRNA.

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CloneID	ODi36894
Clone ID Related Accession (Same CDS sequence)	XM_015157120.1 , XM_015157120.2
Accession Version	XM_015157120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012606.1
CDS	195..6044
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157120.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157120.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157121.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012607.1 Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG22771), mRNA.

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CloneID	ODi36895
Clone ID Related Accession (Same CDS sequence)	XM_015157121.1
Accession Version	XM_015157121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012607.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157121.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157121.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157122.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012608.1 Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG22771), mRNA.

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CloneID	ODi36896
Clone ID Related Accession (Same CDS sequence)	XM_015157122.1 , XM_015157122.2
Accession Version	XM_015157122.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012608.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157122.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157122.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157122.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012608.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X9, mRNA.

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CloneID	ODi36896
Clone ID Related Accession (Same CDS sequence)	XM_015157122.1 , XM_015157122.2
Accession Version	XM_015157122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012608.1
CDS	284..6172
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157122.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157122.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157123.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012609.1 Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG22771), mRNA.

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CloneID	ODi36897
Clone ID Related Accession (Same CDS sequence)	XM_015157123.1
Accession Version	XM_015157123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012609.1
CDS	98..5947
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	XM_015157123.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157123.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157124.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012610.1 Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG22771), mRNA.

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CloneID	ODi36898
Clone ID Related Accession (Same CDS sequence)	XM_015157124.1
Accession Version	XM_015157124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012610.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157124.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157124.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157125.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012611.1 Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG22771), mRNA.

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CloneID	ODi36899
Clone ID Related Accession (Same CDS sequence)	XM_015157125.1 , XM_015157125.2
Accession Version	XM_015157125.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012611.1
CDS	119..6007
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157125.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157125.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157125.2 ) cDNA ORF clone, Drosophila erecta -> XP_015012611.1 Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X8, mRNA.

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CloneID	ODi36899
Clone ID Related Accession (Same CDS sequence)	XM_015157125.1 , XM_015157125.2
Accession Version	XM_015157125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	myosin heavy chain, muscle isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012611.1
CDS	195..6083
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157125.2
Organism	Drosophila erecta
Definition	Drosophila erecta myosin heavy chain, muscle (LOC6547749), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157125.2

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157126.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012612.1 Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG22771), mRNA.

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CloneID	ODi36900
Clone ID Related Accession (Same CDS sequence)	XM_015157126.1
Accession Version	XM_015157126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform H
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012612.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157126.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157126.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CACATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157127.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012613.1 Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG22771), mRNA.

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CloneID	ODi36901
Clone ID Related Accession (Same CDS sequence)	XM_015157127.1
Accession Version	XM_015157127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012613.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157127.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157127.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATAAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCC 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157128.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012614.1 Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG22771), mRNA.

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CloneID	ODi36902
Clone ID Related Accession (Same CDS sequence)	XM_015157128.1
Accession Version	XM_015157128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform J
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012614.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKDMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157128.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157128.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGACATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT TACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATAAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCTAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCCGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157129.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012615.1 Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG22771), mRNA.

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CloneID	ODi36903
Clone ID Related Accession (Same CDS sequence)	XM_015157129.1
Accession Version	XM_015157129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform K
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012615.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157129.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157129.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCCGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6547749 ( XM_015157130.1 ) cDNA ORF clone, Drosophila erecta -> XP_015012616.1 Drosophila erecta uncharacterized protein, transcript variant L (Dere\GG22771), mRNA.

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CloneID	ODi36904
Clone ID Related Accession (Same CDS sequence)	XM_015157130.1
Accession Version	XM_015157130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform L
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015012616.1
CDS	98..5986
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDETAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVAGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTSLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANGEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	XM_015157130.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant L (Dere\GG22771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157130.1

ORF Insert Sequence:

ATGCCGAAGC CAGTAGCCAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CCAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCCTACAAG CGCTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTAATTG CGTACTTCGC CACCGTTGGT 
GCCTCCAAGA AGACCGATGA GACCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAATC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGCCCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCC CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TTTTCTACCA GATCATGTCT GGCTCCGTTG CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA TGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGCCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAGATC 
TTCGCCGATC ACGCCGGACA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GCTCTACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAGATGAAG 
CAACCTGGTG TGGTCGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTATCC CGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCTCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCCTGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAGCAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAACCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTCCGCAAG GAGCTGGAGG CCCTCAACGC CAAGCTGTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GTCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGACC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGCGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCTGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAGCTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGT 
GAGCTTGAGG AGTTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAAATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAGCTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA CCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGATG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTC 
AGCAAGGCCA ACGGTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA GGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCTGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAGG CCGAGAAGGA CGAGCTCCAG 
GCCGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGCGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC CAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAGCAGG CCGATCGCGG CCGTCGCCAG 
GCCGAGCAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAGGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGTGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCCGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATCCAGAAGC TGGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACTTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CCGAGGAAAT CGCTGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGTGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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gene@genscript.com

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1-877-436-7274

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Online Quote Submission

LOC6547749 cDNA ORF clone, Drosophila erecta

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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