DDB_G0283995 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DDB_G0283995
Entrez Gene ID	8624328
Full Name	hypothetical protein
Synonyms	DDBDRAFT_0218554,DDB_0218554
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_633712.1	XP_638804.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB_G0283995 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB_G0283995 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm07866	XM_633712.1 Latest version!	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0283995) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDB_G0283995 ( XM_633712.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_638804.1 Dictyostelium discoideum AX4 hypothetical protein (DDB_G0283995) mRNA, complete cds.

Price & Availability↑

CloneID	ODm07866
Clone ID Related Accession (Same CDS sequence)	XM_633712.1
Accession Version	XM_633712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007090). COMPLETENESS: incomplete on both ends.

ATGAAATTTG GGACTTTCTT TTTATTTTTA TTTTTGTTTA TTTCAATTTC ATCATGTGAA 
TTACCTTTAA AAGAAAAGCA AGCAATAGAT ACTTTATTAC AAATATATGG TTTAAATTTA 
ATAAACGGTG ATTATTGTGG TTCACCCCAA TATTTTGTGT GTAATTTTGA AAACACAACG 
ATTACAGAAA TTTCATTAAG TGGTAATGAA ATTAATAAAA CTATTTTCCC AGATGGAGTA 
ATTGGTAATT TTAGTTTTAT AAATAAAGTA AAATTATCAA ATTCAAATAT CTCAAATGCT 
TTTTTTAGTG AATTACAAAC CACAAGAAAT TTTCAATTTG ATAATTGTTT TATTCCAAAA 
TTTCCAACAT TCCTCCCCTA TGTCCAAGTA TTATATATGA ATGATGTATT TTTTAATGGT 
AATATAAATT ATTCATCATT TTCAGCTATC GGTAATTTTA CATTGATTTA TTCTTCAGGT 
GATTTAATGA CAGATTATCA ATTGATTGAT GATTCACCAC TTTATTTTGT TATTTCAAAC 
ATTAAAATCG TATTAAATAA TATTATAGAT TTTCAGTATT CACTTCAAAA AATAGAAATT 
TATTTAGGAA AATATTTTAA TAATAGTGAT AGTTTTAGAA ATATGAGCTT CACTGGTGGC 
CCAATTTTCA TTAGTGATTT GTACTATAAT AAACCTATCG AAATTACAAA TTTAATTGGT 
CAAAGTGTAA TATTAAATTT TGCTAATGTC CAGTTTAGTT TACCGGATAA TAGCTACATG 
GATTTTACTT TCCATTTGGT TAGTTCTTTA ATTTTTACTA ATTGCTCAGG ATTAACAAAT 
TCAAATGGTG ATTTAATGAT AAAAATAGGA AGTGTTAAAA CTTTAACAAT AACCAATTCG 
AAATTAAAAA AGATACCTCC ATTTTCATTT TATAAAACTT TATCTTCATT GGATATTAGT 
AAAAATAATA TTATAGGTCA GATACCTGAA TTATATGAAC CAATGAATGC ATCACAAATA 
ATTGGTATTA ATTTAAGTGG AAATAAAATA AGTGGTGAAT TAAATCAAAA TCATTGTTAT 
CACAATTTAA ATATATCCAA TAACCAATTA ACAGGTAAAC TTCCAATGTG TTTTATATGT 
CAATTAAATG ATCCACTATT AAGATATAGA ATAAATGGAA ATAATTTTGA AAATTATATT 
AGTAATAGTG AAAATAATTT TCCAATTTGT TCAGGCATAT CATTTATTGG GGATGCTTTG 
ATAGAAGTTG GAACAACTAA TTCATTTATT AATGGAACTA ATTTAGGATG GGAATCTATA 
TATTATAGTA TTCCAGGACA AATATTAAGT GATCCATTGG GATTATTTTT TTCAGTGGAG 
ATTCCAAATA AAGTTTTAAG AATTTCAGAT CCAAAAAAAT TATTCCCAAT TGATAAAGCA 
ATTGTGAATT TCGCAATTCC AAATATAAAT GTTACAATGC ATTTTAAATA CTCTGAACCA 
AAAGTATATG CTATTACAGT GTATCCATAT TATAATCTTG GTTATTTATT TTTAATTAAT 
GGTGCTTCAT TTCCATCCCA AAATAGCACT TCCTCAAGAG TTTCTGTAAA ATTTGACAAT 
TTTGATTGTG AACCAACAAT AGTTGCCGGT ATTGTTATTG AATGTATTGT ATATAAATCT 
CAGTTAGCTG AAAAAATCTA TACAGTATCG ATAACCAATG AACAAACTAG AAAAACTGGT 
TTTTCTAAAT ATAAATTTGA AAGAGTTTAT CCTTTTGTTT ATGCATATAG CTCACCAACG 
AAAATTGGTG GTGTTGTAAC ATTTTTTGGA AGTTATGGGT CAGTCTTTAC CAATGCAACT 
ATTAAAATTG GAAAAGGTAC ATGTGAAATT ATCAATATAA ACTCAACAGT AATTACATGT 
AACCTAGCAG GAGCTAATTC AATTGGTCCA AGTCCTGCAA ATGTTAGTAT AACAATTAAT 
CAAGTTAATT GGTTTGTAAA TGATTATTTC ATTTATAATG AAGATAATAT ATGTCCAGGA 
ACACCAGAAT GTAGTGGCAA TGGTATTTGT TTTAGTGGTT ATTGTGCATG TTCAAATGGT 
TATTCTGGTA TTAAATGTGA TAAAATTTTT ACAGGCGATA TAGAGGTTAA AAAGAATGAT 
ACTTTAACAG AAATGATAAA GAATGGTTAT TCATTTGGTT TTTCAATTAG AGATATTAGA 
GAAATTGATT TCGATGGTAA AACAGTTAGA CAACATAATT TCACAAGTTG GACATTAACA 
CCAGATTCAA CGATTATGAA ATGGACTTAT GTAAATAAAT TTGAAAATTC AATCATTTCA 
TATACAGTAG AACAAATTAC AGGTGCACCA AAGAATTATA CATTTGCAGG TGAACAATTT 
ACACTTCAAC CTGGTTCTTT AAAATTATCA GCAAACATTT CAAATTGGGA ATATTTAGGA 
TCACTAAATA CTCTACAACT TCAAATTCAT TCTTCAGTTA AAGCTCAACA AGAGAATGAA 
TGTGAACAAA AATCTGAAAT TCAATCAAAT GGTAATGGTG TATCATTAAA TTATATTACT 
ATTCAAAAAG ATAAAAATGT ATTCAGTGGT AGATTCATAG ATAAGGTGGT TTCAGATGGT 
AGACCTACCT TTTCAAAAGT TTCAATCTCT GAACAAACTG AAGATTCAAT CACAGTTTCA 
ATATCATTAC CTTATTGTAA AGAATGTTTA ATTGATCCTG ATTTCTCTGT AATGATCAAT 
CCAAATCCAA TATTTAATCC ATGTGACAAT GATCAACCTT CTAATTTAAA ATGGATTATA 
CCTGTTAGTG TAATATTAGG TTTGTTAGGT TTGTTTGCTA TTTTTGTAAT AGTTTTCTTT 
TTAGCACGTG ATCGTATTTA TATCTCTAAA AAAGGTGGAA TTATTTTATT AAAAAAACAA 
AAAAAAACAT CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_638804.1
CDS	1..2958
Translation	MKFGTFFLFLFLFISISSCELPLKEKQAIDTLLQIYGLNLINGDYCGSPQYFVCNFENTTITEISLSGNEINKTIFPDGVIGNFSFINKVKLSNSNISNAFFSELQTTRNFQFDNCFIPKFPTFLPYVQVLYMNDVFFNGNINYSSFSAIGNFTLIYSSGDLMTDYQLIDDSPLYFVISNIKIVLNNIIDFQYSLQKIEIYLGKYFNNSDSFRNMSFTGGPIFISDLYYNKPIEITNLIGQSVILNFANVQFSLPDNSYMDFTFHLVSSLIFTNCSGLTNSNGDLMIKIGSVKTLTITNSKLKKIPPFSFYKTLSSLDISKNNIIGQIPELYEPMNASQIIGINLSGNKISGELNQNHCYHNLNISNNQLTGKLPMCFICQLNDPLLRYRINGNNFENYISNSENNFPICSGISFIGDALIEVGTTNSFINGTNLGWESIYYSIPGQILSDPLGLFFSVEIPNKVLRISDPKKLFPIDKAIVNFAIPNINVTMHFKYSEPKVYAITVYPYYNLGYLFLINGASFPSQNSTSSRVSVKFDNFDCEPTIVAGIVIECIVYKSQLAEKIYTVSITNEQTRKTGFSKYKFERVYPFVYAYSSPTKIGGVVTFFGSYGSVFTNATIKIGKGTCEIININSTVITCNLAGANSIGPSPANVSITINQVNWFVNDYFIYNEDNICPGTPECSGNGICFSGYCACSNGYSGIKCDKIFTGDIEVKKNDTLTEMIKNGYSFGFSIRDIREIDFDGKTVRQHNFTSWTLTPDSTIMKWTYVNKFENSIISYTVEQITGAPKNYTFAGEQFTLQPGSLKLSANISNWEYLGSLNTLQLQIHSSVKAQQENECEQKSEIQSNGNGVSLNYITIQKDKNVFSGRFIDKVVSDGRPTFSKVSISEQTEDSITVSISLPYCKECLIDPDFSVMINPNPIFNPCDNDQPSNLKWIIPVSVILGLLGLFAIFVIVFFLARDRIYISKKGGIILLKKQKKTST

Target ORF information:

RefSeq Version	XM_633712.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0283995) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_633712.1

ORF Insert Sequence:

ATGAAATTTG GGACTTTCTT TTTATTTTTA TTTTTGTTTA TTTCAATTTC ATCATGTGAA 
TTACCTTTAA AAGAAAAGCA AGCAATAGAT ACTTTATTAC AAATATATGG TTTAAATTTA 
ATAAACGGTG ATTATTGTGG TTCACCCCAA TATTTTGTGT GTAATTTTGA AAACACAACG 
ATTACAGAAA TTTCATTAAG TGGTAATGAA ATTAATAAAA CTATTTTCCC AGATGGAGTA 
ATTGGTAATT TTAGTTTTAT AAATAAAGTA AAATTATCAA ATTCAAATAT CTCAAATGCT 
TTTTTTAGTG AATTACAAAC CACAAGAAAT TTTCAATTTG ATAATTGTTT TATTCCAAAA 
TTTCCAACAT TCCTCCCCTA TGTCCAAGTA TTATATATGA ATGATGTATT TTTTAATGGT 
AATATAAATT ATTCATCATT TTCAGCTATC GGTAATTTTA CATTGATTTA TTCTTCAGGT 
GATTTAATGA CAGATTATCA ATTGATTGAT GATTCACCAC TTTATTTTGT TATTTCAAAC 
ATTAAAATCG TATTAAATAA TATTATAGAT TTTCAGTATT CACTTCAAAA AATAGAAATT 
TATTTAGGAA AATATTTTAA TAATAGTGAT AGTTTTAGAA ATATGAGCTT CACTGGTGGC 
CCAATTTTCA TTAGTGATTT GTACTATAAT AAACCTATCG AAATTACAAA TTTAATTGGT 
CAAAGTGTAA TATTAAATTT TGCTAATGTC CAGTTTAGTT TACCGGATAA TAGCTACATG 
GATTTTACTT TCCATTTGGT TAGTTCTTTA ATTTTTACTA ATTGCTCAGG ATTAACAAAT 
TCAAATGGTG ATTTAATGAT AAAAATAGGA AGTGTTAAAA CTTTAACAAT AACCAATTCG 
AAATTAAAAA AGATACCTCC ATTTTCATTT TATAAAACTT TATCTTCATT GGATATTAGT 
AAAAATAATA TTATAGGTCA GATACCTGAA TTATATGAAC CAATGAATGC ATCACAAATA 
ATTGGTATTA ATTTAAGTGG AAATAAAATA AGTGGTGAAT TAAATCAAAA TCATTGTTAT 
CACAATTTAA ATATATCCAA TAACCAATTA ACAGGTAAAC TTCCAATGTG TTTTATATGT 
CAATTAAATG ATCCACTATT AAGATATAGA ATAAATGGAA ATAATTTTGA AAATTATATT 
AGTAATAGTG AAAATAATTT TCCAATTTGT TCAGGCATAT CATTTATTGG GGATGCTTTG 
ATAGAAGTTG GAACAACTAA TTCATTTATT AATGGAACTA ATTTAGGATG GGAATCTATA 
TATTATAGTA TTCCAGGACA AATATTAAGT GATCCATTGG GATTATTTTT TTCAGTGGAG 
ATTCCAAATA AAGTTTTAAG AATTTCAGAT CCAAAAAAAT TATTCCCAAT TGATAAAGCA 
ATTGTGAATT TCGCAATTCC AAATATAAAT GTTACAATGC ATTTTAAATA CTCTGAACCA 
AAAGTATATG CTATTACAGT GTATCCATAT TATAATCTTG GTTATTTATT TTTAATTAAT 
GGTGCTTCAT TTCCATCCCA AAATAGCACT TCCTCAAGAG TTTCTGTAAA ATTTGACAAT 
TTTGATTGTG AACCAACAAT AGTTGCCGGT ATTGTTATTG AATGTATTGT ATATAAATCT 
CAGTTAGCTG AAAAAATCTA TACAGTATCG ATAACCAATG AACAAACTAG AAAAACTGGT 
TTTTCTAAAT ATAAATTTGA AAGAGTTTAT CCTTTTGTTT ATGCATATAG CTCACCAACG 
AAAATTGGTG GTGTTGTAAC ATTTTTTGGA AGTTATGGGT CAGTCTTTAC CAATGCAACT 
ATTAAAATTG GAAAAGGTAC ATGTGAAATT ATCAATATAA ACTCAACAGT AATTACATGT 
AACCTAGCAG GAGCTAATTC AATTGGTCCA AGTCCTGCAA ATGTTAGTAT AACAATTAAT 
CAAGTTAATT GGTTTGTAAA TGATTATTTC ATTTATAATG AAGATAATAT ATGTCCAGGA 
ACACCAGAAT GTAGTGGCAA TGGTATTTGT TTTAGTGGTT ATTGTGCATG TTCAAATGGT 
TATTCTGGTA TTAAATGTGA TAAAATTTTT ACAGGCGATA TAGAGGTTAA AAAGAATGAT 
ACTTTAACAG AAATGATAAA GAATGGTTAT TCATTTGGTT TTTCAATTAG AGATATTAGA 
GAAATTGATT TCGATGGTAA AACAGTTAGA CAACATAATT TCACAAGTTG GACATTAACA 
CCAGATTCAA CGATTATGAA ATGGACTTAT GTAAATAAAT TTGAAAATTC AATCATTTCA 
TATACAGTAG AACAAATTAC AGGTGCACCA AAGAATTATA CATTTGCAGG TGAACAATTT 
ACACTTCAAC CTGGTTCTTT AAAATTATCA GCAAACATTT CAAATTGGGA ATATTTAGGA 
TCACTAAATA CTCTACAACT TCAAATTCAT TCTTCAGTTA AAGCTCAACA AGAGAATGAA 
TGTGAACAAA AATCTGAAAT TCAATCAAAT GGTAATGGTG TATCATTAAA TTATATTACT 
ATTCAAAAAG ATAAAAATGT ATTCAGTGGT AGATTCATAG ATAAGGTGGT TTCAGATGGT 
AGACCTACCT TTTCAAAAGT TTCAATCTCT GAACAAACTG AAGATTCAAT CACAGTTTCA 
ATATCATTAC CTTATTGTAA AGAATGTTTA ATTGATCCTG ATTTCTCTGT AATGATCAAT 
CCAAATCCAA TATTTAATCC ATGTGACAAT GATCAACCTT CTAATTTAAA ATGGATTATA 
CCTGTTAGTG TAATATTAGG TTTGTTAGGT TTGTTTGCTA TTTTTGTAAT AGTTTTCTTT 
TTAGCACGTG ATCGTATTTA TATCTCTAAA AAAGGTGGAA TTATTTTATT AAAAAAACAA 
AAAAAAACAT CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

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DDB_G0283995 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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