ORF » Species Summary » Dictyostelium discoideum AX4 » brf1 cDNA ORF clone

brf1 cDNA ORF clone, Dictyostelium discoideum AX4

The following brf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODm06607 XM_635799.1
Latest version!		 Dictyostelium discoideum AX4 TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 (brf1) mRNA, complete cds. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    brf1 ( XM_635799.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_640891.1 Dictyostelium discoideum AX4 TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 (brf1) mRNA, complete cds.
    Price & Availability↑
    CloneID ODm06607
    Clone ID Related Accession (Same CDS sequence) XM_635799.1
    Accession Version XM_635799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1264608000000
    Organism Dictyostelium discoideum AX4
    Product TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007089). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAAAAA ATTGTGGAAA TTGTGGAAAT GATTCATTTG CGTCATCAAA TGAAGGTAGT 
    GTTGTATGTA CAGCATGTGG TACAGTTAAA GAATCAGCAA ACATTGTATC AGAAGTACAA 
    TTTGGAGGAG ATTCATCAAT TGTTGGTACA TTTGTTAGTG CAACTAGGAA ACCATCATCC 
    TATAGATCAT TAGGAGGAAG AGATTCAAGG GCAATGTCAG TAGAGAATGC ACGTAAAAAA 
    TTGGATCAAA TTGGTAACTC ATTAAGAATT AGAAGTCATT TAATCGATAG TGCACAAAGA 
    ACCTTTGAAT TGGCAATGGA GAATAATTTC ACCAAAGGTA GACAAACAAG ATTGGTTGCA 
    GCAGCATGTT TATATATAGT TTGTCGTAGA GAGAGAACAC CTCATCTACT AATTGACTTT 
    TCAGAGAATC TTCAAGTCAA TGTATTCATA GTTGCTGGTA CATTCCTTCA ATTGATCAAA 
    TTATTAAATA TTCAATTACC AATTGTAGAT CCAAGTTTCT TCATTCAAAG ATTTGCATCA 
    GCCTTAGAGT TTGGTGATCA AACTAAAGAT GTAATTGCGA CCGCTAATAA ATTAGTCGCT 
    AGAATGAAGA GAGATTGGAT GTCAATTGGT AGAAAGCCAT CAGGTATTTG TGGTGCTTCA 
    TTATATATCG CTTCAAAAAT TCATGGTTTC AAAAGATCAA TGAAAGAAAT CGTTCATGTC 
    GTTAAAATTG GTGAACAAAC TTTAATCAAT CGTTTAGATG AATTTAGAAA AACACCAACT 
    GCTACCCTTA GATTCGATGA ATTCAATGGT ATGGATTTAG AGGATCAATG TGATCCACCC 
    TCTTTCACAA GAGCAAGATT AAAAGAAAAG AAAGAAGAAG AAAAATTAAA ACTTTTAGAA 
    GAAAAAAAAG AAAGAAAAAG ATTAAAAAAA GAGAAAAAAA AATTACTTGA ACTTGAAAAT 
    AATAAAGATA ATAAAGAAAT AATTATAAAA GAAAATAAAG AAGAAATAGA GAAAAAATTG 
    GAAAAAAAAC AAGACTTAAA AAAAGATAAA AAAGATAAAA TTAAAGGAGG TGAAGGTGAA 
    GAGGAAGAAG AAGAAGAGGA AGATGATGAT GACGATGATG AAGAGGAGGA AGAAGATAAT 
    TTAAATGAAG AAACTAAAGG TGATAATAAG AAGAATAAAA AAAGAAAGAG AGAATTATTA 
    AAAGATGATG ATGATATTGA AAAAGAATCA AATGATATAG TCAAAGGTTC ACAAAATGTT 
    CAATCTATTA AGGATATTAC AAATGTAGAT TCAATTCCAA CAACACTTTT GGAGAATATT 
    GAAAAGTTTG AAAAAATTCA ATCCGATATT AAAACTTTAG CTCAAAAAGT AAAAAATGAA 
    ATGGATGAAA AAATTAAAGA AAGTCAAAGT CAAGAACCAG ATACAACACC ACCACCATCA 
    TCAACAACAA CCACACAAAC AACAACAACT ACAACTACAA GTCAAAATAA TGAGAGTTCA 
    GCACCAATAG ATTTACCAAC ACCAATTAAT AAAACAATGG ATTCTGTTGT ATCATACCCA 
    ACTATAACAT CAACAATTGA TCAATCAGTT TTTGCACCAA ATGATGAATT GGAAAATTTA 
    AGTGATGAAG AATTAGATTT TTACTTGGAA CATGATAAAG ATACTGTAAA AAAGAAAGAA 
    GAGATTTGGG TGGAATTAAA TAAAGAATGG ATTGAAAAGA ATGAAGAAAG ACAAAGAGAG 
    ATTGAGGAGG ATATTAAAGC TGGTAGACCA CCAAGAAAGA GAAAAGCACC TGTAAGTAAG 
    AAATCTGCTG AAAATGCACA AGCTGCCGCT GAAGAAGAAT TAAAAAAGAG AACTAGAAAT 
    GCAAAACTCG TTGAGAAATT AGGTCTCTTC AAACCAAATT TCGTTACTAT GGGTTTATTT 
    GGTGGGGCTG GTGCATCAAA TAATCAAGCT GCCGTAGGTG CAATGTTATC TACTGATGGC 
    ACCTCTAAAA ATACCTCTAT CAATCCAAAA GAAACAAATA AAGAAGACGA TGATGATGAT 
    GATTATGAAC CAATTCAACC AATTCAATCT GGTAGTTTAT TAAGTCGTAA TTTTAATAAC 
    TATGAAGAAG ATGATTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_640891.1
    CDS1..2121
    Translation

    Target ORF information:

    RefSeq Version XM_635799.1
    Organism Dictyostelium discoideum AX4
    Definition Dictyostelium discoideum AX4 TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 (brf1) mRNA, complete cds.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_635799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAAAAA ATTGTGGAAA TTGTGGAAAT GATTCATTTG CGTCATCAAA TGAAGGTAGT 
    GTTGTATGTA CAGCATGTGG TACAGTTAAA GAATCAGCAA ACATTGTATC AGAAGTACAA 
    TTTGGAGGAG ATTCATCAAT TGTTGGTACA TTTGTTAGTG CAACTAGGAA ACCATCATCC 
    TATAGATCAT TAGGAGGAAG AGATTCAAGG GCAATGTCAG TAGAGAATGC ACGTAAAAAA 
    TTGGATCAAA TTGGTAACTC ATTAAGAATT AGAAGTCATT TAATCGATAG TGCACAAAGA 
    ACCTTTGAAT TGGCAATGGA GAATAATTTC ACCAAAGGTA GACAAACAAG ATTGGTTGCA 
    GCAGCATGTT TATATATAGT TTGTCGTAGA GAGAGAACAC CTCATCTACT AATTGACTTT 
    TCAGAGAATC TTCAAGTCAA TGTATTCATA GTTGCTGGTA CATTCCTTCA ATTGATCAAA 
    TTATTAAATA TTCAATTACC AATTGTAGAT CCAAGTTTCT TCATTCAAAG ATTTGCATCA 
    GCCTTAGAGT TTGGTGATCA AACTAAAGAT GTAATTGCGA CCGCTAATAA ATTAGTCGCT 
    AGAATGAAGA GAGATTGGAT GTCAATTGGT AGAAAGCCAT CAGGTATTTG TGGTGCTTCA 
    TTATATATCG CTTCAAAAAT TCATGGTTTC AAAAGATCAA TGAAAGAAAT CGTTCATGTC 
    GTTAAAATTG GTGAACAAAC TTTAATCAAT CGTTTAGATG AATTTAGAAA AACACCAACT 
    GCTACCCTTA GATTCGATGA ATTCAATGGT ATGGATTTAG AGGATCAATG TGATCCACCC 
    TCTTTCACAA GAGCAAGATT AAAAGAAAAG AAAGAAGAAG AAAAATTAAA ACTTTTAGAA 
    GAAAAAAAAG AAAGAAAAAG ATTAAAAAAA GAGAAAAAAA AATTACTTGA ACTTGAAAAT 
    AATAAAGATA ATAAAGAAAT AATTATAAAA GAAAATAAAG AAGAAATAGA GAAAAAATTG 
    GAAAAAAAAC AAGACTTAAA AAAAGATAAA AAAGATAAAA TTAAAGGAGG TGAAGGTGAA 
    GAGGAAGAAG AAGAAGAGGA AGATGATGAT GACGATGATG AAGAGGAGGA AGAAGATAAT 
    TTAAATGAAG AAACTAAAGG TGATAATAAG AAGAATAAAA AAAGAAAGAG AGAATTATTA 
    AAAGATGATG ATGATATTGA AAAAGAATCA AATGATATAG TCAAAGGTTC ACAAAATGTT 
    CAATCTATTA AGGATATTAC AAATGTAGAT TCAATTCCAA CAACACTTTT GGAGAATATT 
    GAAAAGTTTG AAAAAATTCA ATCCGATATT AAAACTTTAG CTCAAAAAGT AAAAAATGAA 
    ATGGATGAAA AAATTAAAGA AAGTCAAAGT CAAGAACCAG ATACAACACC ACCACCATCA 
    TCAACAACAA CCACACAAAC AACAACAACT ACAACTACAA GTCAAAATAA TGAGAGTTCA 
    GCACCAATAG ATTTACCAAC ACCAATTAAT AAAACAATGG ATTCTGTTGT ATCATACCCA 
    ACTATAACAT CAACAATTGA TCAATCAGTT TTTGCACCAA ATGATGAATT GGAAAATTTA 
    AGTGATGAAG AATTAGATTT TTACTTGGAA CATGATAAAG ATACTGTAAA AAAGAAAGAA 
    GAGATTTGGG TGGAATTAAA TAAAGAATGG ATTGAAAAGA ATGAAGAAAG ACAAAGAGAG 
    ATTGAGGAGG ATATTAAAGC TGGTAGACCA CCAAGAAAGA GAAAAGCACC TGTAAGTAAG 
    AAATCTGCTG AAAATGCACA AGCTGCCGCT GAAGAAGAAT TAAAAAAGAG AACTAGAAAT 
    GCAAAACTCG TTGAGAAATT AGGTCTCTTC AAACCAAATT TCGTTACTAT GGGTTTATTT 
    GGTGGGGCTG GTGCATCAAA TAATCAAGCT GCCGTAGGTG CAATGTTATC TACTGATGGC 
    ACCTCTAAAA ATACCTCTAT CAATCCAAAA GAAACAAATA AAGAAGACGA TGATGATGAT 
    GATTATGAAC CAATTCAACC AATTCAATCT GGTAGTTTAT TAAGTCGTAA TTTTAATAAC 
    TATGAAGAAG ATGATTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the social amoeba Dictyostelium discoideum.
    Nature435(7038)43-57(2005 May)
    Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A


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