DG1112 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DG1112
Entrez Gene ID	8620536
Full Name	RasGTPase-activating protein
Synonyms	DDBDRAFT_0185082,DDBDRAFT_0217813,DDB_0185082,DDB_0217813
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_638038.1	XP_643130.1	RasGTPase-activating protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Glöckner G, Eichinger L, Szafranski K, Pachebat JA, Bankier AT, Dear PH, Lehmann R, Baumgart C, Parra G, Abril JF, Guigó R, Kumpf K, Tunggal B, Cox E, Quail MA, Platzer M, Rosenthal A, Noegel AA,
Nature418(6893)79-85(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DG1112 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DG1112 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm04321	XM_638038.1 Latest version!	Dictyostelium discoideum AX4 RasGTPase-activating protein (DG1112) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DG1112 ( XM_638038.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_643130.1 Dictyostelium discoideum AX4 RasGTPase-activating protein (DG1112) mRNA, complete cds.

Price & Availability↑

CloneID	ODm04321
Clone ID Related Accession (Same CDS sequence)	XM_638038.1
Accession Version	XM_638038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	RasGTPase-activating protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007088). COMPLETENESS: incomplete on both ends.

ATGATTGAAT TATCAACTAC ATCACCAGCA TATAATGCTA TGGTTTCATA TGAAAATCAA 
ACCTCAAAAG CAAGTTCAAA TACAATCATA GTTTATAAAC CAATATCAGA TCATATTGTA 
TTGGATGGTA TGGTTTTAAC AGAAGATGAA ATTCAACAAC GTCAAAGAGA AAAAACAGAA 
ACATTAATGT ATTCAACAAA AGATGCTGTA ACAATTGATT CAATATTTAA ACATATTGTA 
AATAATCAAA TTAATATAAT TCATGAATTT TATAAATTTA TTGAAAAAGA TCAATATGAA 
TCAATGTCAA AATTAATTAT TACATTTTCA ATTGGATGTA ATAATGCATT AAGAATATTA 
AGATATTTTA TATATACAGA TATTAAAGAG GATGTTAATA ATATTAATAG AAATTATTCA 
TTTTCATCAT TGTTAATATC AAATTATTTG GAGAAAACAT GTCAACAATA TATAAAGGAT 
GTATTGAGAC AATTTGTATT ACAATTAGTT CAAGATAAAG CAATAAATTT TAAATATGAT 
TTAGAGAATT CAGAGAATGT GGTGATTCAT AATGAATTTA GTAATTTAAT AGTGTGGAGT 
AATCGATTGA GTGAGATCAT CACTAGTCAA GAATCATTGG ATAAAATGCC AATGTCGATT 
AAAGTGATTG CAATGTTTTT CCATGAAATA TGGCCACAAG ATGATCAAGG TGGTCAAGGT 
GTTTCCAATT TCTTGATTAA TAAATTATTT GTACCAGCAT TGATTTCACC ACAACTCTAT 
GACTTGATAC CACCGCAAAT TCATTTAAAT AGTAGATCAA CATCCAATTT ATCAATACTT 
GCAAGATACT TGTCAACTTG TATTACAACT TATTCATCTG ATGAATATCA AGAAGTATTC 
GATTGTAATC AATCATTGTT ACAATGTAAA CAAAAAATAA AACAAACTTT TAAAGAGAGT 
CAATATTTTA AATCTGTATT ATCAGCTAGG TTCTCTACAC AATCTTATCA ATATACCAAT 
GAAGCTCCAA TGAGCCAATT ACATTTCCTT CATCGTATAC TTTGTAATGA GAAATCAACA 
ATACGTCAAA TGTATCAATC TATGGAAGCA GCATCTGAAT TTGAAAAGTT GATGGATTTA 
TTAGCAGATT ATAACTATCG TGTATCCTAT CAATTGTTAA CAACACCATC AGAGATTAAA 
TCTGTTAAAT CTTATATGGA TTCAAAGAAT GAGGAAATCT CATACATTGG TTATATTCAA 
AAGAATGGAA AGAAAGGTTT TGTAAAGAGA ATATTGGTAG TTGGTATCTA TAGAATTGTA 
ACATTTAAAA TGAATGGTAA ATTGGCAAGA GACGGCCATA TATTGGATTT ACAAGAGATT 
GTATCACAAA ATAATCAACA ATATGAATTG ATTTTCAAGG GTCATCGTAT TCATGCAACC 
ACTGAGGAAT GTGATCATAC CATCACATGT ATTCGTCGTA TCTATGAGTA TTGTCTTTAC 
AATTGGCCAT ATGCTTTGAA AATGAAATTA AAGATTCAAC CATCATCTCG TTTGGAGGCA 
ATGTCACCAC CTGACCATAC GCCAACCTCG GCAATGGTGG GTGCATACAA ATGCCTTTGC 
TCCTACTATT CCATACCAAT TAAACAAACC ATAGCTTGGT ATATCGAACA TGCATTTAAA 
GATCCAAAAT ATAAAGTATT CAATTTGAAA GTTTTCACTA AACATACACG TGACCTACCA 
ACCAATCAAG AATTGATACC ATTAATTCAT TCATTACGTT ACGATTGGTT CTTTGAAGAA 
TTCATCATTA AAGAATATAA ATTGGATAAT AAAGATTTAT TAAATGAAAT TGGCTCAATG 
TTATCATCAA ATTCAACAAT CAATAAATTA ATTCTCTCAA ATTTATCATT ACCAAAGGAA 
TTTATGATGA CATTATTCGA TACGATTCAG TCACAGAAAT CTTTGAAATT GACTTTATTA 
GATATTAGTA ATAATCAATT GGATGATAAA TCATTAAATT CTTTCTTTTC ATATCTTCAA 
TATGGTAATA CCAATGGTTT GATTCATTTG AATTGTCAAT CTATTGGAAT TAAAGAATCA 
CATATTAAAC CATTCATAGA TTCACTTAGA AGAGGTAATT TAAATTCTTT AACAAGTTTA 
AATATTTCAA ATAATAAAAT CAATGACCAA GCTATGGAAT TATCGAAATG GTTAACTAAT 
GAAGGTAAAT CTTTGAAAAA TTTAGATTTA TCTGAAACTT TATTAAAAAT TAAATTCTTT 
CAATTTAATG ATCAATCATC TTGTTTAGAG AATTTAAATT TAAATAGAAA TTTATTTCGT 
TCAAAAGAGG ATACACAATC ACTTTCAAAC TGTTTATCAA ATTTACCAAA TTTATTAACC 
CTATCATTAA ATAAAACAAT GATACCATCA GATTCAATTA AAGAGATTTT ATCATTTTTG 
TGTGGTGCTA ATAATAATAA TAATAATAAA AATGAATTAT TTTCAAATTT AATAATATCA 
GAAAATTCAA TTCAAAGACC ATTGATTACA AATATGATCT CAATGAATGC TTTTAATTTA 
ATTAAGGAAA TCGATTTTTC TGATAATGAT TTAACCGATG GTGGTATTAA AGAACTTTGT 
CGTTCATTAT ATGGTAATCA AATAATTACA AGTTTAAATA TAAATGGTTG TTTTAGAGGT 
AGTGCCGGTG CTCAAAGAAG TAAGGCAATC TCTGCTTTGT CATCTTTCAT TAATTCAAGT 
TCATCATTGG AAACATTGTT AATGAGAGGT GGTCAAAAAT CAAATCAACA ATTACAACGT 
TGTATTGTAC CTTTGTTATT ATCATTGGCT GCAAATTTAA ATACTCGTTT AAAGAAATTG 
GATATCTCAA ATCATCAATT CTCAAATTTC GGTGCTAATG CTCTAAGTAA AGCTATCATT 
TTAAATAAAT CAATCTCTAC TCTAATTTTA GATAATAATT CAATTGGTAC AATTGGTTAT 
GCAAATTTAA AGAATTCATT AAAACAAAAT TATACTATCA AAGAATTACC AATTCCATTA 
TTTGATTTAA CTGAAACTTT AAAATATGGT GGTACATCTT TAATTGAACA AAAGAAATTA 
CGTTCTTTAT TATTTAAAAT TGAATTATAT TTATCAAGAA ATCAATCATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_643130.1
CDS	1..3174
Translation	MIELSTTSPAYNAMVSYENQTSKASSNTIIVYKPISDHIVLDGMVLTEDEIQQRQREKTETLMYSTKDAVTIDSIFKHIVNNQINIIHEFYKFIEKDQYESMSKLIITFSIGCNNALRILRYFIYTDIKEDVNNINRNYSFSSLLISNYLEKTCQQYIKDVLRQFVLQLVQDKAINFKYDLENSENVVIHNEFSNLIVWSNRLSEIITSQESLDKMPMSIKVIAMFFHEIWPQDDQGGQGVSNFLINKLFVPALISPQLYDLIPPQIHLNSRSTSNLSILARYLSTCITTYSSDEYQEVFDCNQSLLQCKQKIKQTFKESQYFKSVLSARFSTQSYQYTNEAPMSQLHFLHRILCNEKSTIRQMYQSMEAASEFEKLMDLLADYNYRVSYQLLTTPSEIKSVKSYMDSKNEEISYIGYIQKNGKKGFVKRILVVGIYRIVTFKMNGKLARDGHILDLQEIVSQNNQQYELIFKGHRIHATTEECDHTITCIRRIYEYCLYNWPYALKMKLKIQPSSRLEAMSPPDHTPTSAMVGAYKCLCSYYSIPIKQTIAWYIEHAFKDPKYKVFNLKVFTKHTRDLPTNQELIPLIHSLRYDWFFEEFIIKEYKLDNKDLLNEIGSMLSSNSTINKLILSNLSLPKEFMMTLFDTIQSQKSLKLTLLDISNNQLDDKSLNSFFSYLQYGNTNGLIHLNCQSIGIKESHIKPFIDSLRRGNLNSLTSLNISNNKINDQAMELSKWLTNEGKSLKNLDLSETLLKIKFFQFNDQSSCLENLNLNRNLFRSKEDTQSLSNCLSNLPNLLTLSLNKTMIPSDSIKEILSFLCGANNNNNNKNELFSNLIISENSIQRPLITNMISMNAFNLIKEIDFSDNDLTDGGIKELCRSLYGNQIITSLNINGCFRGSAGAQRSKAISALSSFINSSSSLETLLMRGGQKSNQQLQRCIVPLLLSLAANLNTRLKKLDISNHQFSNFGANALSKAIILNKSISTLILDNNSIGTIGYANLKNSLKQNYTIKELPIPLFDLTETLKYGGTSLIEQKKLRSLLFKIELYLSRNQSL

Target ORF information:

RefSeq Version	XM_638038.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 RasGTPase-activating protein (DG1112) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_638038.1

ORF Insert Sequence:

ATGATTGAAT TATCAACTAC ATCACCAGCA TATAATGCTA TGGTTTCATA TGAAAATCAA 
ACCTCAAAAG CAAGTTCAAA TACAATCATA GTTTATAAAC CAATATCAGA TCATATTGTA 
TTGGATGGTA TGGTTTTAAC AGAAGATGAA ATTCAACAAC GTCAAAGAGA AAAAACAGAA 
ACATTAATGT ATTCAACAAA AGATGCTGTA ACAATTGATT CAATATTTAA ACATATTGTA 
AATAATCAAA TTAATATAAT TCATGAATTT TATAAATTTA TTGAAAAAGA TCAATATGAA 
TCAATGTCAA AATTAATTAT TACATTTTCA ATTGGATGTA ATAATGCATT AAGAATATTA 
AGATATTTTA TATATACAGA TATTAAAGAG GATGTTAATA ATATTAATAG AAATTATTCA 
TTTTCATCAT TGTTAATATC AAATTATTTG GAGAAAACAT GTCAACAATA TATAAAGGAT 
GTATTGAGAC AATTTGTATT ACAATTAGTT CAAGATAAAG CAATAAATTT TAAATATGAT 
TTAGAGAATT CAGAGAATGT GGTGATTCAT AATGAATTTA GTAATTTAAT AGTGTGGAGT 
AATCGATTGA GTGAGATCAT CACTAGTCAA GAATCATTGG ATAAAATGCC AATGTCGATT 
AAAGTGATTG CAATGTTTTT CCATGAAATA TGGCCACAAG ATGATCAAGG TGGTCAAGGT 
GTTTCCAATT TCTTGATTAA TAAATTATTT GTACCAGCAT TGATTTCACC ACAACTCTAT 
GACTTGATAC CACCGCAAAT TCATTTAAAT AGTAGATCAA CATCCAATTT ATCAATACTT 
GCAAGATACT TGTCAACTTG TATTACAACT TATTCATCTG ATGAATATCA AGAAGTATTC 
GATTGTAATC AATCATTGTT ACAATGTAAA CAAAAAATAA AACAAACTTT TAAAGAGAGT 
CAATATTTTA AATCTGTATT ATCAGCTAGG TTCTCTACAC AATCTTATCA ATATACCAAT 
GAAGCTCCAA TGAGCCAATT ACATTTCCTT CATCGTATAC TTTGTAATGA GAAATCAACA 
ATACGTCAAA TGTATCAATC TATGGAAGCA GCATCTGAAT TTGAAAAGTT GATGGATTTA 
TTAGCAGATT ATAACTATCG TGTATCCTAT CAATTGTTAA CAACACCATC AGAGATTAAA 
TCTGTTAAAT CTTATATGGA TTCAAAGAAT GAGGAAATCT CATACATTGG TTATATTCAA 
AAGAATGGAA AGAAAGGTTT TGTAAAGAGA ATATTGGTAG TTGGTATCTA TAGAATTGTA 
ACATTTAAAA TGAATGGTAA ATTGGCAAGA GACGGCCATA TATTGGATTT ACAAGAGATT 
GTATCACAAA ATAATCAACA ATATGAATTG ATTTTCAAGG GTCATCGTAT TCATGCAACC 
ACTGAGGAAT GTGATCATAC CATCACATGT ATTCGTCGTA TCTATGAGTA TTGTCTTTAC 
AATTGGCCAT ATGCTTTGAA AATGAAATTA AAGATTCAAC CATCATCTCG TTTGGAGGCA 
ATGTCACCAC CTGACCATAC GCCAACCTCG GCAATGGTGG GTGCATACAA ATGCCTTTGC 
TCCTACTATT CCATACCAAT TAAACAAACC ATAGCTTGGT ATATCGAACA TGCATTTAAA 
GATCCAAAAT ATAAAGTATT CAATTTGAAA GTTTTCACTA AACATACACG TGACCTACCA 
ACCAATCAAG AATTGATACC ATTAATTCAT TCATTACGTT ACGATTGGTT CTTTGAAGAA 
TTCATCATTA AAGAATATAA ATTGGATAAT AAAGATTTAT TAAATGAAAT TGGCTCAATG 
TTATCATCAA ATTCAACAAT CAATAAATTA ATTCTCTCAA ATTTATCATT ACCAAAGGAA 
TTTATGATGA CATTATTCGA TACGATTCAG TCACAGAAAT CTTTGAAATT GACTTTATTA 
GATATTAGTA ATAATCAATT GGATGATAAA TCATTAAATT CTTTCTTTTC ATATCTTCAA 
TATGGTAATA CCAATGGTTT GATTCATTTG AATTGTCAAT CTATTGGAAT TAAAGAATCA 
CATATTAAAC CATTCATAGA TTCACTTAGA AGAGGTAATT TAAATTCTTT AACAAGTTTA 
AATATTTCAA ATAATAAAAT CAATGACCAA GCTATGGAAT TATCGAAATG GTTAACTAAT 
GAAGGTAAAT CTTTGAAAAA TTTAGATTTA TCTGAAACTT TATTAAAAAT TAAATTCTTT 
CAATTTAATG ATCAATCATC TTGTTTAGAG AATTTAAATT TAAATAGAAA TTTATTTCGT 
TCAAAAGAGG ATACACAATC ACTTTCAAAC TGTTTATCAA ATTTACCAAA TTTATTAACC 
CTATCATTAA ATAAAACAAT GATACCATCA GATTCAATTA AAGAGATTTT ATCATTTTTG 
TGTGGTGCTA ATAATAATAA TAATAATAAA AATGAATTAT TTTCAAATTT AATAATATCA 
GAAAATTCAA TTCAAAGACC ATTGATTACA AATATGATCT CAATGAATGC TTTTAATTTA 
ATTAAGGAAA TCGATTTTTC TGATAATGAT TTAACCGATG GTGGTATTAA AGAACTTTGT 
CGTTCATTAT ATGGTAATCA AATAATTACA AGTTTAAATA TAAATGGTTG TTTTAGAGGT 
AGTGCCGGTG CTCAAAGAAG TAAGGCAATC TCTGCTTTGT CATCTTTCAT TAATTCAAGT 
TCATCATTGG AAACATTGTT AATGAGAGGT GGTCAAAAAT CAAATCAACA ATTACAACGT 
TGTATTGTAC CTTTGTTATT ATCATTGGCT GCAAATTTAA ATACTCGTTT AAAGAAATTG 
GATATCTCAA ATCATCAATT CTCAAATTTC GGTGCTAATG CTCTAAGTAA AGCTATCATT 
TTAAATAAAT CAATCTCTAC TCTAATTTTA GATAATAATT CAATTGGTAC AATTGGTTAT 
GCAAATTTAA AGAATTCATT AAAACAAAAT TATACTATCA AAGAATTACC AATTCCATTA 
TTTGATTTAA CTGAAACTTT AAAATATGGT GGTACATCTT TAATTGAACA AAAGAAATTA 
CGTTCTTTAT TATTTAAAAT TGAATTATAT TTATCAAGAA ATCAATCATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Nature418(6893)79-85(2002 Jul)
Glöckner G,Eichinger L,Szafranski K,Pachebat JA,Bankier AT,Dear PH,Lehmann R,Baumgart C,Parra G,Abril JF,Guigó R,Kumpf K,Tunggal B,Cox E,Quail MA,Platzer M,Rosenthal A,Noegel AA,

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DG1112 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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