prpf8 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	prpf8
Entrez Gene ID	8619668
Full Name	Mov34/MPN/PAD-1 domain-containing protein
Synonyms	DDBDRAFT_0167592,DDBDRAFT_0233128,DDB_0167592,DDB_0233128
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_639148.1	XP_644240.1	Mov34/MPN/PAD-1 domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Glöckner G, Eichinger L, Szafranski K, Pachebat JA, Bankier AT, Dear PH, Lehmann R, Baumgart C, Parra G, Abril JF, Guigó R, Kumpf K, Tunggal B, Cox E, Quail MA, Platzer M, Rosenthal A, Noegel AA,
Nature418(6893)79-85(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm03518	XM_639148.1 Latest version!	Dictyostelium discoideum AX4 pre-mRNA processing factor 8 (prpf8) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prpf8 ( XM_639148.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_644240.1 Dictyostelium discoideum AX4 pre-mRNA processing factor 8 (prpf8) mRNA, complete cds.

Price & Availability↑

CloneID	ODm03518
Clone ID Related Accession (Same CDS sequence)	XM_639148.1
Accession Version	XM_639148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	Mov34/MPN/PAD-1 domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007088). COMPLETENESS: incomplete on both ends.

ATGGATGATA CTAATAGTAA TATTAATCAA TCAAATGAAT CACAACATTT AGAAGAAAAA 
GCAAAAAAAT GGATTCAATT AAATAATAAA AAATATAGTG AAAAAAGAAA ATTTGGAGCT 
GTAGAGATTA GAAAAGAGGA TATGCCTCCA GAGCATTTAA GAAAGATTAT AAAAGATCAT 
GGTGATATGT CAAATAGAAG GTTTAGAGAT GATAAACGTG TTTATTTAGG AGCATTGAAA 
TATATGCCAC ATGCAATTTT AAAACTTTTA GAGAATATAC CAATGCCATG GGAACAAGTT 
AAATATGTTA AAGTATTATA TCATTTATCA GGTGCAATCA CATTTGTAAA TGAAATACCA 
TTCGTAATTG AACCAATCTA TATTGCACAA TGGGCAACTA TGTGGGTTAC AATGAGAAGA 
GAGAAAAGGG ATCGTACCCA TTTTCGTCGT ATGAAATTCC CATTATTCGA TGATGAAGAA 
CCACCATTAG ATTACTCTGA TAACATTTTA GATAATGAAG TTGAAGATCC AATTCAAATG 
GAATTAGATG AAAATGATGA TTCTGAAGTA ATAGATTGGT TATATGATTC AAAACCATTA 
GTTAATACAA AATTTGTAAA TGGTTCAAGT TATAGAAAAT GGAGATTAAA TTTACCAATT 
ATGTCAACAT TATTTAGATT GGCATCACCA TTATTAAGTG ATTTAACAGA TAGTAATTAT 
TTTTATTTAT TTGATGATAA TAGTTTTTTC ACATCAAAAG CATTGAATAT GGCAATACCA 
GGTGGACCAA AGTTTGAACC ATTATTTAGG GATGTTGATG ATGATGATGA AGATTGGAAT 
GAATTCAATG ATATCAATAA AGTGATTATT AGAAATAAGA TTAGAACAGA ATATAAAATT 
GCATTCCCAT ATCTATACAA TAGTAGACCA CGTAAAGTTA AAACACCAAC TTATCATACT 
CCAAATAATT GTTATATTAA AAATGATTCA CCAGATTTAC CTGGTTTTTA TTTTGGTGCT 
GCATTAAATC CAATTCCATC ATATAAAACA AGTGGTAATA AAAATGAACA ATCTGAATAT 
GGTACAGAAG ATGATGAATT TCAATTACCA GAAGAAATTG AAACAATATT ATCAAAAACA 
GAGATTGAAC ATGATAATTT AGCAAATGGT ATACAATTAT ATTGGGCACC AAGACCATTT 
TCATTAAGAT CAGGTACAAC TAGAAGAGCA GAGGATATTC CATTAGTTAA ATCATGGTAT 
AAAGAACATT GTCCAAGTGA ACATCCAGTT AAAGTTAGAG TTAGTTATCA AAAGTTATTG 
AAATGTCATG TATTGAATAA ATTACATCAT CGTAAACCAA AAGCACAAAC TAAAAGAAAT 
TTATTTAAAT CATTGAAAGC AACTAAATTC TTTCAATCTA CAGAGATTGA TTGGGTAGAG 
GCAGGTTTAC AAGTTTGTCG TCAAGGTTAC AATATGTTAA ATCTATTAAT TCATAGAAAG 
AATTTGAATT ATTTACATTT GGATTACAAT TTCTATTTGA AACCAATTAA AACTCTAACA 
ACAAAGGAGA GAAAGAAATC AAGATTTGGT AATGCATTCC ATTTGTGTAG AGAGATATTA 
CGTTTAACTA AATTGGTGGT TGATGTTCAT GTGAAATTTA GATTAGGTGA TGCAGATGCT 
TTTCAATTGG CAGATGCTAT TCAATATTTA TTTAGTCATT TGGGTCTATT GACTGGTATG 
TATAAATACA AGTATCGTTT AATGAGACAA ATTCGTATGT GTAAAGATTT GAAACATTTA 
ATCTACTATC GTTTCAATAC TGGCGCTGTT GGTAAAGGTC CAGGTTGTGG TTTTTGGGCA 
CCAATGTGGA GAGTTTGGTT ATTCTTTTTA CGTGGTATAG TACCTTTGTT GGAACGTTGG 
CTTGGAAATC TATTGGCAAG ACAATTCGAA GGTAGACAAA CCAAAGGTAT GGCCAAAACA 
GTCACTAAAC AACGTGTTGA ATCTCATTTC GATTATGAAT TACGTGCAGC AGTAATGCAT 
GATATCCTCG ATATGATGCC AGAAGGAATC AAAGCAAATA AATCACGTAT TATCTTACAA 
CATCTTTCAG AGGCTTGGAG ATGTTGGAAA TCAAATATCC CATGGAAAGT ACCAGGTTTA 
CCAATACCAA TAGAGAATAT GATTCTACGT TATGTTAAAT CAAAAGCAGA TTGGTGGACA 
AATATAGCTC ATTACAATCG TGAACGTATT AAACGTGGTG CTACCATCGA TAAAACTGCA 
AGTAAAAAGA ATTTGGGTCG TTTAACTAGA CTTTGGTTAA AGGCAGAACA AGAACGTCAA 
CATAATTATC TAAAGGATGG TCCATACGTA TCGGCAGAGG AAGCTGTTGC CATTTATACC 
ACCACTGTTC ATTGGTTGGA GAAACGTAGA TTCTCTGCGA TTCCATTCCC TCAAACTTCA 
TACAAACACG ATATTAAAAT TCTCACATTG GCATTGGAAC GTCTTAAAGA GGCATACAGT 
GTTAAATCAC GTTTAAATCA ATCACAACGT GAGGAGTTAT CATTGGTTGA ACAAGCTTAT 
GATAATCCTC ATGATGCATT AGCTCGTATC AAAAGACATT TACTCACTCA ACGTACCTTT 
AAAGAGGTTG GTATTGAATT TATGGATATG TACACTCATC TAGTACCAAT CTATGATGTA 
GATCCATTTG AAAAGATCAC CGATGCCTAT TTGGATCAAT ATCTATGGTA TGAAGCAGAT 
AAACGTCAAC TCTTTCCAAA TTGGGTTAAA CCATCCGATA ATGAACCACC ACCTGTACTC 
ATTCATAAAT GGTGTCAAGG TATTAATAAT TTAGATCAAG TTTGGGAGAC TAGTCAAGGT 
GAATGTGTTG TTTTATTAGA GACTCAATTT AGTAAAGTTT ATGAAAAGAT GGATTTAACT 
TTGATGAATC GTTTGTTACG TTTAATTGTT GATCAAAATA TTGCCGATTA TATGTCTGGT 
AAGAATAATG TAGTAATCAA CTATAAGGAT ATGAATCATA CCAACTCTTA TGGTTTAATT 
AGAGGTTTAC AATTTGCATC ATTTATTTTC CAATATTATG GTTTGGTATT GGATCTTTTA 
GTATTGGGTT TAGAACGTGC ATCAGCACTT GCAGGACCAC CAAACTTACC AAATTCATTC 
CTTACATTCC CATCAGTTCA AACTGAAACC GCCCATCCAA TTCGTTTGTA TAGTCGTTAT 
GTTGATCGTA TTCATGTACT CTATAAATTT ACAGCGGATG AAGCAAGAAA ATTAATTCAA 
AAGTATATGT CAGAACATCC AGATCCAAAC AATGAAAATG TGGTTGGTTA TAACAATAAG 
AAATGTTGGC CACGTGATTG TCGTATGCGT TTAATGAAAC ATGATGTTAA CTTGGGTAGA 
GCAGTATTTT GGCAAATTAA AAATAGATTA CCACGTTCAT TGACAACCAT TGATTGGGAG 
GATTCATTCG TATCGGTCTA TAGTAAAGAT AATCCAAATC TATTGATGAA TATGGCAGGT 
TTCGATATTC GTATACTTCC AAAATGTCGT ACACCCTTGG ATCAATTGGC ACCAAAGGAT 
GCAGTTTGGA GTCTTCAAAA TGTTAATACC AAAGAACGTA CGGCTCAAGC ATTCCTTAGG 
GTCGATACAG AATCACAAGA ACGTTTTGAA AATCGTATTC GTATGATCCT CATGGCAAGT 
GGTTCAACAA CATTCACAAA GATTGTAAAT AAATGGAATA CAGCATTGAT TGGTTTAATG 
ACTTATTATC GTGAGGCGGT GGTTACAACT CGTGAAATGT TGGATATTTT AGTGCGTTGT 
GAAAATAAAA TTCAAACTCG TGTAAAGATT GGATTGAATA GTAAGATGCC AAATCGTTTC 
CCACCAGTGG TATTCTATAC ACCAAAGGAG TTGGGTGGTT TAGGTATGTT AAGTATGGGT 
CATGTATTGA TTCCACAATC GGATTTGAAA TATTCAAAAC AAACCGATAC TGGTATAACT 
CATTTCACAA GTGGTATGAG TCATGATGAA GATCAATTAA TTCCAAATCT TTATCGTTAC 
ATTCAACCAT GGGAACAAGA AATTAAAGAT AGTCAACGTG TTTGGGCAGA ATATGCAATC 
AAATATGAAG AGGCTAAATC ACAAAATAAG AATCTTACAT TGGAGGATCT TGAAGATAGT 
TGGGATCGTG GTATACCACG TATCAATACA TTATTCCAAA AGAGTCGTCA TACTTTAGCT 
TATGATAAAG GTTGGAGAGT AAGAACCGAT TGGAAACAAT ATCAAGTCCT AAAGAATAAT 
CCATTCTGGT GGACTAATCA ACGTCATGAT GGTAAACTTT GGAATCTTAA TAATTATCGT 
ACAGATATCA TTCAAGCATT GGGTGGTGTT GAAGGTATTC TAGAGCATAC TCTATTCAAA 
GGTACTTATT TCCCAACTTG GGAAGGTTTA TTTTGGGAGA AAGCATCAGG TTTCGAAGAG 
TCTATGAAAT ATAAAAAACT TACACACGCT CAACGTTCAG GTCTTAATCA AATTCCAAAT 
CGTCGTTTCA CACTTTGGTG GTCTCCAACT ATCAATCGTA AGAATGTTTA TGTTGGTTTC 
CAAGTTCAAT TGGATTTAAC AGGTATTTTC ATGCATGGTA AAATTCCAAC ACTAAAGATT 
TCATTGATTC AAATTTTCCG TGCACATCTT TGGCAAAAGA TTCATGAATC ATTAGTAATG 
GATTTATGTC AAGTTTTCGA TCAAGAATTG GATAATCTAG AGATATCAGT AGTAAATAAA 
GAAGCAATTC ATCCACGTAA ATCTTATAAA ATGAATTCAT CATGTGCTGA TATTCTTTTA 
CGTGCAACTC ATAAATGGCA AGTTAGTCGT CCATCATTGT TAAATGATAA TCGTGATACC 
TATGATAATA CCACCACTCA ATATTGGTTG GATGTTCAAT TGAAATGGGG TGATTTCGAT 
TCTCATGATA TCGAGAGATA CAGTCGTGCC AAATTCTTGG ATTATACAAC CGATAGTATG 
TCATTGTATC CATCACCAAC TGGTTGTTTG ATTGGTTTAG ATTTGGCTTA CAATATCTAC 
AGTTCCTTTG GTAATTGGTT CCTTGGTGTT AAACCTTTGG TTCAAAAAGC AATGGCAAAA 
ATTTTGAAAT CCAATCCTGC ACTCTATGTA CTACGTGAAC GTATTCGTAA AGGTTTACAA 
TTATATTCAT CCGAACCAAC TGAACCTTAC TTATCAAGTC AAAACTTTGG TGAATTATTT 
AGTAATAAAA TTATGTGGTT CGTTGATGAT TCCAATGTTT ATCGTGTAAC CATTCATAAA 
ACTTTTGAAG GTAATTTAAC AACTAAACCA ATCAATGGTG CAATTTTCAT TTTCAATCCA 
CGTACTGGTC AATTATTCCT CAAGATCATT CATACCGATG TTTGGTTAGG TCAAAAACGT 
TTAGGTCAAT TAGCAAAATG GAAGACTGCC GAGGAGGTTG CTGCATTAAT TCGTTCCTTA 
CCAGTGGAAG AACAACCAAA ACAAATCATT GCCACTCGTA AAGGTATGAT GGATCCATTG 
GAGGTACATC TTTTAGATTT CCCAAATATC GTCATTCAAG GTAGTGAACT TCAATTACCA 
TTCCAAGCTT GTTTGAAAGT AGAGAAGTTT GGTGATCTCA TTTTGAAAGC AACCGAACCA 
AAGATGGTAC TCTTCAATAT CTATGATGAT TGGTTATCAA CAATTCATTC TTACACTGCT 
TTCCTTCGTT TGATTCTAAT CTTACGTGCA CTTCATGTCA ATTTAGAACG TACTAAAATC 
ATATTGAAAC CAAATAAAAA TGTTATCACT CAACCTCATC ATATTTGGCC AACTCTAACA 
GAACAAGAAT GGTTAACCGT TGAAGGTTCA TTGAAAGATT TGATTTTAGC AGATTTTGGT 
AAAAGAAATA ATGTAAACGT TGCTTCGCTC ACTCAATCAG AGATTAGAGA TATCATTTTG 
GGTATGGAAA TTTCTGCACC ATCACAACAA CGTGAAGATC AAATCGCAGA GATTGAAAAA 
CAAAAAACAG AAGCATCTCA TTTAACAGCC GTTACAGTTC GTTCAACCAA TATTCATGGT 
GAAGAGATTA TCACAACCGC TACATCACCA CATGAACAAA AAGTATTCTC TTCAAAAACA 
GATTGGCGTG TACGTGCTAT CAGTGCAACT AATCTTCATT TACGTACCAA TCAAATCTAT 
GTAAACTCTG ATAACGCTAA AGAAACTGGT GGTTTCACTT ATGTTTTCCC AAAGAATATC 
TTAAAGAAAT TCATTACCAT TGCCGATCTT CGTACTCAAA TCATGGGTTA TTGTTATGGT 
ATCTCACCAC CCGATAATCC ATCAGTAAAA GAAATTCGTT GTATTGTAAT GCCACCACAA 
TGGGGTACAC CAGTTCATGT AACCGTACCA AATCAATTAC CAGAACATGA ATATCTCAAA 
GATTTAGAAC CATTGGGTTG GATTCACACT CAACCAACTG AATTACCACA ACTATCACCA 
CAAGATGTTA TCACTCATTC AAAGATTATG TCTGACAATA AGAGTTGGGA TGGTGAGAAA 
ACTGTTATCA TCTCTGTCTC TGTGGCTTGG CCTTGTACTC TAACTGCTTA CCATTTGACT 
CCAAGTGGTT TTGAATGGGG TAAAAATAAT AAAGATTCTC TCAATTATCA AGGTTATCAA 
CCACAATTCT ATGAGAAGGT TCAAATGTTA TTATCCGATC GTTTCCTTGG TTTCTATATG 
GTACCTGATC GTGGTTCTTG GAATTATAAT TTCATGGGTG TTAAACATTC CACAAATATG 
ACCTATGGTT TGAAATTAGA TTATCCTAAA AACTTTTATG ATGAATCTCA TAGACCTGCT 
CATTTCCAAA ATTGGACTCA AATGGCTCCT TCAGCAAATG ATGATGAAGA AAATCAACCT 
GAAAATGAAA ATTTATTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_644240.1
CDS	1..6984
Translation	MDDTNSNINQSNESQHLEEKAKKWIQLNNKKYSEKRKFGAVEIRKEDMPPEHLRKIIKDHGDMSNRRFRDDKRVYLGALKYMPHAILKLLENIPMPWEQVKYVKVLYHLSGAITFVNEIPFVIEPIYIAQWATMWVTMRREKRDRTHFRRMKFPLFDDEEPPLDYSDNILDNEVEDPIQMELDENDDSEVIDWLYDSKPLVNTKFVNGSSYRKWRLNLPIMSTLFRLASPLLSDLTDSNYFYLFDDNSFFTSKALNMAIPGGPKFEPLFRDVDDDDEDWNEFNDINKVIIRNKIRTEYKIAFPYLYNSRPRKVKTPTYHTPNNCYIKNDSPDLPGFYFGAALNPIPSYKTSGNKNEQSEYGTEDDEFQLPEEIETILSKTEIEHDNLANGIQLYWAPRPFSLRSGTTRRAEDIPLVKSWYKEHCPSEHPVKVRVSYQKLLKCHVLNKLHHRKPKAQTKRNLFKSLKATKFFQSTEIDWVEAGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFYLKPIKTLTTKERKKSRFGNAFHLCREILRLTKLVVDVHVKFRLGDADAFQLADAIQYLFSHLGLLTGMYKYKYRLMRQIRMCKDLKHLIYYRFNTGAVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRQTKGMAKTVTKQRVESHFDYELRAAVMHDILDMMPEGIKANKSRIILQHLSEAWRCWKSNIPWKVPGLPIPIENMILRYVKSKADWWTNIAHYNRERIKRGATIDKTASKKNLGRLTRLWLKAEQERQHNYLKDGPYVSAEEAVAIYTTTVHWLEKRRFSAIPFPQTSYKHDIKILTLALERLKEAYSVKSRLNQSQREELSLVEQAYDNPHDALARIKRHLLTQRTFKEVGIEFMDMYTHLVPIYDVDPFEKITDAYLDQYLWYEADKRQLFPNWVKPSDNEPPPVLIHKWCQGINNLDQVWETSQGECVVLLETQFSKVYEKMDLTLMNRLLRLIVDQNIADYMSGKNNVVINYKDMNHTNSYGLIRGLQFASFIFQYYGLVLDLLVLGLERASALAGPPNLPNSFLTFPSVQTETAHPIRLYSRYVDRIHVLYKFTADEARKLIQKYMSEHPDPNNENVVGYNNKKCWPRDCRMRLMKHDVNLGRAVFWQIKNRLPRSLTTIDWEDSFVSVYSKDNPNLLMNMAGFDIRILPKCRTPLDQLAPKDAVWSLQNVNTKERTAQAFLRVDTESQERFENRIRMILMASGSTTFTKIVNKWNTALIGLMTYYREAVVTTREMLDILVRCENKIQTRVKIGLNSKMPNRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLKYSKQTDTGITHFTSGMSHDEDQLIPNLYRYIQPWEQEIKDSQRVWAEYAIKYEEAKSQNKNLTLEDLEDSWDRGIPRINTLFQKSRHTLAYDKGWRVRTDWKQYQVLKNNPFWWTNQRHDGKLWNLNNYRTDIIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTHAQRSGLNQIPNRRFTLWWSPTINRKNVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESLVMDLCQVFDQELDNLEISVVNKEAIHPRKSYKMNSSCADILLRATHKWQVSRPSLLNDNRDTYDNTTTQYWLDVQLKWGDFDSHDIERYSRAKFLDYTTDSMSLYPSPTGCLIGLDLAYNIYSSFGNWFLGVKPLVQKAMAKILKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNFGELFSNKIMWFVDDSNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTDVWLGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIATRKGMMDPLEVHLLDFPNIVIQGSELQLPFQACLKVEKFGDLILKATEPKMVLFNIYDDWLSTIHSYTAFLRLILILRALHVNLERTKIILKPNKNVITQPHHIWPTLTEQEWLTVEGSLKDLILADFGKRNNVNVASLTQSEIRDIILGMEISAPSQQREDQIAEIEKQKTEASHLTAVTVRSTNIHGEEIITTATSPHEQKVFSSKTDWRVRAISATNLHLRTNQIYVNSDNAKETGGFTYVFPKNILKKFITIADLRTQIMGYCYGISPPDNPSVKEIRCIVMPPQWGTPVHVTVPNQLPEHEYLKDLEPLGWIHTQPTELPQLSPQDVITHSKIMSDNKSWDGEKTVIISVSVAWPCTLTAYHLTPSGFEWGKNNKDSLNYQGYQPQFYEKVQMLLSDRFLGFYMVPDRGSWNYNFMGVKHSTNMTYGLKLDYPKNFYDESHRPAHFQNWTQMAPSANDDEENQPENENLFE

Target ORF information:

RefSeq Version	XM_639148.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 pre-mRNA processing factor 8 (prpf8) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_639148.1

ORF Insert Sequence:

ATGGATGATA CTAATAGTAA TATTAATCAA TCAAATGAAT CACAACATTT AGAAGAAAAA 
GCAAAAAAAT GGATTCAATT AAATAATAAA AAATATAGTG AAAAAAGAAA ATTTGGAGCT 
GTAGAGATTA GAAAAGAGGA TATGCCTCCA GAGCATTTAA GAAAGATTAT AAAAGATCAT 
GGTGATATGT CAAATAGAAG GTTTAGAGAT GATAAACGTG TTTATTTAGG AGCATTGAAA 
TATATGCCAC ATGCAATTTT AAAACTTTTA GAGAATATAC CAATGCCATG GGAACAAGTT 
AAATATGTTA AAGTATTATA TCATTTATCA GGTGCAATCA CATTTGTAAA TGAAATACCA 
TTCGTAATTG AACCAATCTA TATTGCACAA TGGGCAACTA TGTGGGTTAC AATGAGAAGA 
GAGAAAAGGG ATCGTACCCA TTTTCGTCGT ATGAAATTCC CATTATTCGA TGATGAAGAA 
CCACCATTAG ATTACTCTGA TAACATTTTA GATAATGAAG TTGAAGATCC AATTCAAATG 
GAATTAGATG AAAATGATGA TTCTGAAGTA ATAGATTGGT TATATGATTC AAAACCATTA 
GTTAATACAA AATTTGTAAA TGGTTCAAGT TATAGAAAAT GGAGATTAAA TTTACCAATT 
ATGTCAACAT TATTTAGATT GGCATCACCA TTATTAAGTG ATTTAACAGA TAGTAATTAT 
TTTTATTTAT TTGATGATAA TAGTTTTTTC ACATCAAAAG CATTGAATAT GGCAATACCA 
GGTGGACCAA AGTTTGAACC ATTATTTAGG GATGTTGATG ATGATGATGA AGATTGGAAT 
GAATTCAATG ATATCAATAA AGTGATTATT AGAAATAAGA TTAGAACAGA ATATAAAATT 
GCATTCCCAT ATCTATACAA TAGTAGACCA CGTAAAGTTA AAACACCAAC TTATCATACT 
CCAAATAATT GTTATATTAA AAATGATTCA CCAGATTTAC CTGGTTTTTA TTTTGGTGCT 
GCATTAAATC CAATTCCATC ATATAAAACA AGTGGTAATA AAAATGAACA ATCTGAATAT 
GGTACAGAAG ATGATGAATT TCAATTACCA GAAGAAATTG AAACAATATT ATCAAAAACA 
GAGATTGAAC ATGATAATTT AGCAAATGGT ATACAATTAT ATTGGGCACC AAGACCATTT 
TCATTAAGAT CAGGTACAAC TAGAAGAGCA GAGGATATTC CATTAGTTAA ATCATGGTAT 
AAAGAACATT GTCCAAGTGA ACATCCAGTT AAAGTTAGAG TTAGTTATCA AAAGTTATTG 
AAATGTCATG TATTGAATAA ATTACATCAT CGTAAACCAA AAGCACAAAC TAAAAGAAAT 
TTATTTAAAT CATTGAAAGC AACTAAATTC TTTCAATCTA CAGAGATTGA TTGGGTAGAG 
GCAGGTTTAC AAGTTTGTCG TCAAGGTTAC AATATGTTAA ATCTATTAAT TCATAGAAAG 
AATTTGAATT ATTTACATTT GGATTACAAT TTCTATTTGA AACCAATTAA AACTCTAACA 
ACAAAGGAGA GAAAGAAATC AAGATTTGGT AATGCATTCC ATTTGTGTAG AGAGATATTA 
CGTTTAACTA AATTGGTGGT TGATGTTCAT GTGAAATTTA GATTAGGTGA TGCAGATGCT 
TTTCAATTGG CAGATGCTAT TCAATATTTA TTTAGTCATT TGGGTCTATT GACTGGTATG 
TATAAATACA AGTATCGTTT AATGAGACAA ATTCGTATGT GTAAAGATTT GAAACATTTA 
ATCTACTATC GTTTCAATAC TGGCGCTGTT GGTAAAGGTC CAGGTTGTGG TTTTTGGGCA 
CCAATGTGGA GAGTTTGGTT ATTCTTTTTA CGTGGTATAG TACCTTTGTT GGAACGTTGG 
CTTGGAAATC TATTGGCAAG ACAATTCGAA GGTAGACAAA CCAAAGGTAT GGCCAAAACA 
GTCACTAAAC AACGTGTTGA ATCTCATTTC GATTATGAAT TACGTGCAGC AGTAATGCAT 
GATATCCTCG ATATGATGCC AGAAGGAATC AAAGCAAATA AATCACGTAT TATCTTACAA 
CATCTTTCAG AGGCTTGGAG ATGTTGGAAA TCAAATATCC CATGGAAAGT ACCAGGTTTA 
CCAATACCAA TAGAGAATAT GATTCTACGT TATGTTAAAT CAAAAGCAGA TTGGTGGACA 
AATATAGCTC ATTACAATCG TGAACGTATT AAACGTGGTG CTACCATCGA TAAAACTGCA 
AGTAAAAAGA ATTTGGGTCG TTTAACTAGA CTTTGGTTAA AGGCAGAACA AGAACGTCAA 
CATAATTATC TAAAGGATGG TCCATACGTA TCGGCAGAGG AAGCTGTTGC CATTTATACC 
ACCACTGTTC ATTGGTTGGA GAAACGTAGA TTCTCTGCGA TTCCATTCCC TCAAACTTCA 
TACAAACACG ATATTAAAAT TCTCACATTG GCATTGGAAC GTCTTAAAGA GGCATACAGT 
GTTAAATCAC GTTTAAATCA ATCACAACGT GAGGAGTTAT CATTGGTTGA ACAAGCTTAT 
GATAATCCTC ATGATGCATT AGCTCGTATC AAAAGACATT TACTCACTCA ACGTACCTTT 
AAAGAGGTTG GTATTGAATT TATGGATATG TACACTCATC TAGTACCAAT CTATGATGTA 
GATCCATTTG AAAAGATCAC CGATGCCTAT TTGGATCAAT ATCTATGGTA TGAAGCAGAT 
AAACGTCAAC TCTTTCCAAA TTGGGTTAAA CCATCCGATA ATGAACCACC ACCTGTACTC 
ATTCATAAAT GGTGTCAAGG TATTAATAAT TTAGATCAAG TTTGGGAGAC TAGTCAAGGT 
GAATGTGTTG TTTTATTAGA GACTCAATTT AGTAAAGTTT ATGAAAAGAT GGATTTAACT 
TTGATGAATC GTTTGTTACG TTTAATTGTT GATCAAAATA TTGCCGATTA TATGTCTGGT 
AAGAATAATG TAGTAATCAA CTATAAGGAT ATGAATCATA CCAACTCTTA TGGTTTAATT 
AGAGGTTTAC AATTTGCATC ATTTATTTTC CAATATTATG GTTTGGTATT GGATCTTTTA 
GTATTGGGTT TAGAACGTGC ATCAGCACTT GCAGGACCAC CAAACTTACC AAATTCATTC 
CTTACATTCC CATCAGTTCA AACTGAAACC GCCCATCCAA TTCGTTTGTA TAGTCGTTAT 
GTTGATCGTA TTCATGTACT CTATAAATTT ACAGCGGATG AAGCAAGAAA ATTAATTCAA 
AAGTATATGT CAGAACATCC AGATCCAAAC AATGAAAATG TGGTTGGTTA TAACAATAAG 
AAATGTTGGC CACGTGATTG TCGTATGCGT TTAATGAAAC ATGATGTTAA CTTGGGTAGA 
GCAGTATTTT GGCAAATTAA AAATAGATTA CCACGTTCAT TGACAACCAT TGATTGGGAG 
GATTCATTCG TATCGGTCTA TAGTAAAGAT AATCCAAATC TATTGATGAA TATGGCAGGT 
TTCGATATTC GTATACTTCC AAAATGTCGT ACACCCTTGG ATCAATTGGC ACCAAAGGAT 
GCAGTTTGGA GTCTTCAAAA TGTTAATACC AAAGAACGTA CGGCTCAAGC ATTCCTTAGG 
GTCGATACAG AATCACAAGA ACGTTTTGAA AATCGTATTC GTATGATCCT CATGGCAAGT 
GGTTCAACAA CATTCACAAA GATTGTAAAT AAATGGAATA CAGCATTGAT TGGTTTAATG 
ACTTATTATC GTGAGGCGGT GGTTACAACT CGTGAAATGT TGGATATTTT AGTGCGTTGT 
GAAAATAAAA TTCAAACTCG TGTAAAGATT GGATTGAATA GTAAGATGCC AAATCGTTTC 
CCACCAGTGG TATTCTATAC ACCAAAGGAG TTGGGTGGTT TAGGTATGTT AAGTATGGGT 
CATGTATTGA TTCCACAATC GGATTTGAAA TATTCAAAAC AAACCGATAC TGGTATAACT 
CATTTCACAA GTGGTATGAG TCATGATGAA GATCAATTAA TTCCAAATCT TTATCGTTAC 
ATTCAACCAT GGGAACAAGA AATTAAAGAT AGTCAACGTG TTTGGGCAGA ATATGCAATC 
AAATATGAAG AGGCTAAATC ACAAAATAAG AATCTTACAT TGGAGGATCT TGAAGATAGT 
TGGGATCGTG GTATACCACG TATCAATACA TTATTCCAAA AGAGTCGTCA TACTTTAGCT 
TATGATAAAG GTTGGAGAGT AAGAACCGAT TGGAAACAAT ATCAAGTCCT AAAGAATAAT 
CCATTCTGGT GGACTAATCA ACGTCATGAT GGTAAACTTT GGAATCTTAA TAATTATCGT 
ACAGATATCA TTCAAGCATT GGGTGGTGTT GAAGGTATTC TAGAGCATAC TCTATTCAAA 
GGTACTTATT TCCCAACTTG GGAAGGTTTA TTTTGGGAGA AAGCATCAGG TTTCGAAGAG 
TCTATGAAAT ATAAAAAACT TACACACGCT CAACGTTCAG GTCTTAATCA AATTCCAAAT 
CGTCGTTTCA CACTTTGGTG GTCTCCAACT ATCAATCGTA AGAATGTTTA TGTTGGTTTC 
CAAGTTCAAT TGGATTTAAC AGGTATTTTC ATGCATGGTA AAATTCCAAC ACTAAAGATT 
TCATTGATTC AAATTTTCCG TGCACATCTT TGGCAAAAGA TTCATGAATC ATTAGTAATG 
GATTTATGTC AAGTTTTCGA TCAAGAATTG GATAATCTAG AGATATCAGT AGTAAATAAA 
GAAGCAATTC ATCCACGTAA ATCTTATAAA ATGAATTCAT CATGTGCTGA TATTCTTTTA 
CGTGCAACTC ATAAATGGCA AGTTAGTCGT CCATCATTGT TAAATGATAA TCGTGATACC 
TATGATAATA CCACCACTCA ATATTGGTTG GATGTTCAAT TGAAATGGGG TGATTTCGAT 
TCTCATGATA TCGAGAGATA CAGTCGTGCC AAATTCTTGG ATTATACAAC CGATAGTATG 
TCATTGTATC CATCACCAAC TGGTTGTTTG ATTGGTTTAG ATTTGGCTTA CAATATCTAC 
AGTTCCTTTG GTAATTGGTT CCTTGGTGTT AAACCTTTGG TTCAAAAAGC AATGGCAAAA 
ATTTTGAAAT CCAATCCTGC ACTCTATGTA CTACGTGAAC GTATTCGTAA AGGTTTACAA 
TTATATTCAT CCGAACCAAC TGAACCTTAC TTATCAAGTC AAAACTTTGG TGAATTATTT 
AGTAATAAAA TTATGTGGTT CGTTGATGAT TCCAATGTTT ATCGTGTAAC CATTCATAAA 
ACTTTTGAAG GTAATTTAAC AACTAAACCA ATCAATGGTG CAATTTTCAT TTTCAATCCA 
CGTACTGGTC AATTATTCCT CAAGATCATT CATACCGATG TTTGGTTAGG TCAAAAACGT 
TTAGGTCAAT TAGCAAAATG GAAGACTGCC GAGGAGGTTG CTGCATTAAT TCGTTCCTTA 
CCAGTGGAAG AACAACCAAA ACAAATCATT GCCACTCGTA AAGGTATGAT GGATCCATTG 
GAGGTACATC TTTTAGATTT CCCAAATATC GTCATTCAAG GTAGTGAACT TCAATTACCA 
TTCCAAGCTT GTTTGAAAGT AGAGAAGTTT GGTGATCTCA TTTTGAAAGC AACCGAACCA 
AAGATGGTAC TCTTCAATAT CTATGATGAT TGGTTATCAA CAATTCATTC TTACACTGCT 
TTCCTTCGTT TGATTCTAAT CTTACGTGCA CTTCATGTCA ATTTAGAACG TACTAAAATC 
ATATTGAAAC CAAATAAAAA TGTTATCACT CAACCTCATC ATATTTGGCC AACTCTAACA 
GAACAAGAAT GGTTAACCGT TGAAGGTTCA TTGAAAGATT TGATTTTAGC AGATTTTGGT 
AAAAGAAATA ATGTAAACGT TGCTTCGCTC ACTCAATCAG AGATTAGAGA TATCATTTTG 
GGTATGGAAA TTTCTGCACC ATCACAACAA CGTGAAGATC AAATCGCAGA GATTGAAAAA 
CAAAAAACAG AAGCATCTCA TTTAACAGCC GTTACAGTTC GTTCAACCAA TATTCATGGT 
GAAGAGATTA TCACAACCGC TACATCACCA CATGAACAAA AAGTATTCTC TTCAAAAACA 
GATTGGCGTG TACGTGCTAT CAGTGCAACT AATCTTCATT TACGTACCAA TCAAATCTAT 
GTAAACTCTG ATAACGCTAA AGAAACTGGT GGTTTCACTT ATGTTTTCCC AAAGAATATC 
TTAAAGAAAT TCATTACCAT TGCCGATCTT CGTACTCAAA TCATGGGTTA TTGTTATGGT 
ATCTCACCAC CCGATAATCC ATCAGTAAAA GAAATTCGTT GTATTGTAAT GCCACCACAA 
TGGGGTACAC CAGTTCATGT AACCGTACCA AATCAATTAC CAGAACATGA ATATCTCAAA 
GATTTAGAAC CATTGGGTTG GATTCACACT CAACCAACTG AATTACCACA ACTATCACCA 
CAAGATGTTA TCACTCATTC AAAGATTATG TCTGACAATA AGAGTTGGGA TGGTGAGAAA 
ACTGTTATCA TCTCTGTCTC TGTGGCTTGG CCTTGTACTC TAACTGCTTA CCATTTGACT 
CCAAGTGGTT TTGAATGGGG TAAAAATAAT AAAGATTCTC TCAATTATCA AGGTTATCAA 
CCACAATTCT ATGAGAAGGT TCAAATGTTA TTATCCGATC GTTTCCTTGG TTTCTATATG 
GTACCTGATC GTGGTTCTTG GAATTATAAT TTCATGGGTG TTAAACATTC CACAAATATG 
ACCTATGGTT TGAAATTAGA TTATCCTAAA AACTTTTATG ATGAATCTCA TAGACCTGCT 
CATTTCCAAA ATTGGACTCA AATGGCTCCT TCAGCAAATG ATGATGAAGA AAATCAACCT 
GAAAATGAAA ATTTATTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Nature418(6893)79-85(2002 Jul)
Glöckner G,Eichinger L,Szafranski K,Pachebat JA,Bankier AT,Dear PH,Lehmann R,Baumgart C,Parra G,Abril JF,Guigó R,Kumpf K,Tunggal B,Cox E,Quail MA,Platzer M,Rosenthal A,Noegel AA,

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prpf8 cDNA ORF clone, Dictyostelium discoideum AX4

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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