USP43A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP43A
Entrez Gene ID	563983
Full Name	ubiquitin specific peptidase 43a
Synonyms	si:ch211-57c18.1
General protein information	Preferred Names ubiquitin specific peptidase 43a Names ubiquitin carboxyl-terminal hydrolase 43 usp43
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009302356.2	XP_009300631.1	ubiquitin carboxyl-terminal hydrolase 43 isoform X1
XM_009302357.3	XP_009300632.1	ubiquitin carboxyl-terminal hydrolase 43 isoform X2
NM_001089402.1	NP_001082871.1	ubiquitin carboxyl-terminal hydrolase 43
XM_009302356.3	XP_009300631.1	ubiquitin carboxyl-terminal hydrolase 43 isoform X1
XM_009302357.2	XP_009300632.1	ubiquitin carboxyl-terminal hydrolase 43 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	USP43	XP_005220486.1
Mus musculus (house mouse)	Usp43	NP_776115.2
Rattus norvegicus (Norway rat)	Usp43	NP_001119775.1
Pan troglodytes (chimpanzee)	USP43	XP_511843.3
Canis lupus familiaris (dog)	USP43	XP_005620104.1
Macaca mulatta (Rhesus monkey)	USP43	XP_002800319.1
Danio rerio (zebrafish)	LOC100149276	XP_001920291.2
Xenopus tropicalis (tropical clawed frog)	usp43	XP_004918792.1
Homo sapiens (human)	USP43	NP_694942.3
Danio rerio (zebrafish)	usp43a	NP_001082871.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following USP43A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP43A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35731	NM_001089402.1 Latest version!	Danio rerio ubiquitin specific peptidase 43a (usp43a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa44217	XM_009302356.3 Latest version!	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa44218	XM_009302357.3 Latest version!	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa44217	XM_009302356.2	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa44218	XM_009302357.2	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP43A ( NM_001089402.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001082871.1 Danio rerio ubiquitin specific peptidase 43a (usp43a), mRNA.

Price & Availability↑

CloneID	ODa35731
Clone ID Related Accession (Same CDS sequence)	NM_001089402.1
Accession Version	NM_001089402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 43
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX908749.6. On May 23, 2007 this sequence version replaced XM_687346.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGC CCGGAACTCG ACACAGTTTT GTACAGGAGC ACTTCCAAGC CCAGTACAGG 
TCATCCTTGA CATGCCCGCA TTGCCTTAAA CAGAGCAACA CTTTTGACCC TTTCCTGTGT 
ATTTCCCTTC CAATCCCGCT GCGACAGACC AGGGCCCTGA ATGTGACTCT GGTGTTCAGC 
ACAGAAGGTC AGCGGTTTCT GAGAATAGGG CTGGCTGTGC CTCTCTTTGG GTCTATTTCA 
ACACTCAGGG CCATGGTAGC AGAGGAGGGC AAGCTCTCTC CAGACCAGGT AATTTTGACA 
GAGATGAACT CGGGTGGGTT CCAGCGCTCT TTCTCTGATG AAGATGACAT GACCAGTATA 
TCAGAGAGTG ATGTCATCTA CGCCTTCCAG GTTCCCCCTC ACTTCACCAG AGGAGGTTCC 
ATGCGGTTCT CAGATTTCAA AGGTCACAGG CTACCGCCAT CAGGGACGTT ATCAACAGAA 
TTCTTGAATC ATGGGGGCTC TTCAAAAGTC CTTCTCTTAA TCTGCAATGT TGCTGGCTCT 
GGCCAACAGG CTACAAGGTT CGGGCCACCA TTTCTGATGA GAGTAGAAAG AACTCTTTCT 
TGGGATCATC TGCAACAGAG CGTCTGCAAT AAGCTTTACG TTTTTCAAAA AACTGCAATA 
ATAAAGGTTA GTACTACTGA ACTGTTTAAA GTGCGTGTGG TGGGTGGATC AGCCTCCTGC 
AGTTATCTGT CTCCGCACGA CAGCAGACCT CTGTGCCATC CCACCGTAGA CAGAGCCCTC 
AAGCTATGTG GTCCAGGTGG TCCTTTGCAT GTGAAGCTCA TAATTGAATG GGACAACAAA 
ATTAAAGAAT GCCTCTTTGG CAATATTCAA GAGGAGGTGA TTGAGGATGC AGAGAGTGTG 
AGAATCCAGC AGCAGAATCA TGTACAACAG CACAGCTGCA CACTGGATGA CTGTTTTCAG 
CTCTACACCA AAGAGGAGCA GCTGGCACCA GACGATGCAT GGAAGTGCCC CCACTGTAAG 
CAAATGCAGC AGGGAATGGT GAAGATGAGT TTATGGACAC TGCCCGACAT CCTCATTCTG 
CACCTGAAGC GTTTTCGACA GGTGGGCAAA CGGCGAAACA AGCTCTCCAC CCTCATTCGC 
TTTCCTGTAA GTGCTCTGGA TATGACCCCC CATGTGGTGA AACGCAGTCA AAGCATGAAG 
AATCTGGCCC CGTTGCCTTC AACTTGGAAG CATGGAGAAA CTGACTTTCT TTATGACTTG 
TATGCAGTTT GCAACCACCA CGGAGGCATG CATGGAGGCC ATTACACAGC CTATTGCAGG 
AACTCCGTGG ACGGCCACTG GTACAGTTAT GATGACAGCA GTGTGGAGAT TGTGCCAGAG 
GAGGAACTGT GCACACGAGG GGCTTATATC CTGTTCTACC AGAGGAGAAA TGTCATCCCA 
CCCTGGTCTG CCAACAGCTC GGTCAGAGGT TCCACCAGCT CCTCGATGTC AGATCACTGG 
CTCATCCGTT TGAATGGTGA CAGTAAAAGA GGAAGTATGG TTTCACGATC TTCTACAACA 
TGCCCTTCTT CCATCGCTGA CTCCCCAGAG TCACCAGTTT TTAAAAATGA ACCCTCAATT 
GAGGAAAGAG GTGGGTTTGA AAGCAGACCT TTTGTGCGAG GAATCCAAGG TCGTAGTGTA 
AGCATGAGAA GTCCCACCAA GACTAAAGTG CTCCCACTGC GATGGTCTTT TGGAAATAAA 
GACCGCCACA AACCTGTGCC GGCTTCTGAG CAGTCTCCTG CAGAACTGGT GGAGTATTTA 
GAGTCTGGGA GGAGACCAAG ATGTACCAAA GACCCTATTG TTTCCCTAAT GACTCGACCT 
TCAGACAAAG ACAACGATGC AAAAAAAACA GCCAAATCTT CCAGCCACAG TGGCCTCAGA 
CCTCCAGAAT ATCTGAAAAT ACCACAGGGT CAAGAAAGAG GTAGCTCAGA GAAAGCAATG 
GGCCAACAAC CTGCCAGCAG CAGTAGATCA AGAGAAGAAG GGACCTTGAC CAGACGGAGC 
AGCAGAAAAT CTAAACCGGA CCAATCACAC TCCAGGAAGA GCTCTGGAGC AGGGATCCAG 
TCCAGTTGTA GTACTCCAAG TGGCTGGGAA ACAGCACAGA AGCATGAGTC GAGTGACAAG 
CGTGACCAAT CAGAGGATAA ACATAAAAAA GGGCAGGAGA CTAAACGTCA TGATGTCTTC 
GCTTTTCTCA AAGGTGGCTT TATCAAGAAG GATTCCCTTC GGAAGTCTCG GGATAATGAT 
TCTGTCAAAA AGGCTGATTC AGGGGTGAAG AGCCCATCTG GAGTTTCCCT CTCCAACGGG 
ACACATAGTA GAAATCCAGA AGGTAATGTC AGTACTATGA ACCATGGTAG CAGGCACGGA 
AGTGAACTGG CAAATGGCAA GGTGAGTCGT GGTTCCTCAG ACATCAAACG CTCACAAAGC 
TCATCCAATA TTCAAAGCAA AACTGATCTA GGCTTAAGAA GAACAGCATC TTTACACAAA 
AACGGCACAT CGGTGGCCCA ACCACAGAGT GTGACCGGGG ACAGGGGCTC TCAGAGCACT 
TTACAACGCA CACGCTACAG CACAAACTCA CTGGGCAGAA AGAAGGCAGT GCCTGAATCA 
AGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082871.1
CDS	1..3369
Translation	MDSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQPDAAAEDDDGGFGDTASVHNSGNYRESSARKSSSYGSVKERLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMMKSEEVRGEVTDKLASLVRALWTNDYTPQLSVDFKSVVAKYGSQFKGNAQHDALEFLLWLLDCVHEDANLSTNNNQSRNSSSSKPGTRHSFVQEHFQAQYRSSLTCPHCLKQSNTFDPFLCISLPIPLRQTRALNVTLVFSTEGQRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQVILTEMNSGGFQRSFSDEDDMTSISESDVIYAFQVPPHFTRGGSMRFSDFKGHRLPPSGTLSTEFLNHGGSSKVLLLICNVAGSGQQATRFGPPFLMRVERTLSWDHLQQSVCNKLYVFQKTAIIKVSTTELFKVRVVGGSASCSYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECLFGNIQEEVIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMVKMSLWTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSMKNLAPLPSTWKHGETDFLYDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSSVEIVPEEELCTRGAYILFYQRRNVIPPWSANSSVRGSTSSSMSDHWLIRLNGDSKRGSMVSRSSTTCPSSIADSPESPVFKNEPSIEERGGFESRPFVRGIQGRSVSMRSPTKTKVLPLRWSFGNKDRHKPVPASEQSPAELVEYLESGRRPRCTKDPIVSLMTRPSDKDNDAKKTAKSSSHSGLRPPEYLKIPQGQERGSSEKAMGQQPASSSRSREEGTLTRRSSRKSKPDQSHSRKSSGAGIQSSCSTPSGWETAQKHESSDKRDQSEDKHKKGQETKRHDVFAFLKGGFIKKDSLRKSRDNDSVKKADSGVKSPSGVSLSNGTHSRNPEGNVSTMNHGSRHGSELANGKVSRGSSDIKRSQSSSNIQSKTDLGLRRTASLHKNGTSVAQPQSVTGDRGSQSTLQRTRYSTNSLGRKKAVPESSF

Target ORF information:

RefSeq Version	NM_001089402.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 43a (usp43a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089402.1

ORF Insert Sequence:

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGC CCGGAACTCG ACACAGTTTT GTACAGGAGC ACTTCCAAGC CCAGTACAGG 
TCATCCTTGA CATGCCCGCA TTGCCTTAAA CAGAGCAACA CTTTTGACCC TTTCCTGTGT 
ATTTCCCTTC CAATCCCGCT GCGACAGACC AGGGCCCTGA ATGTGACTCT GGTGTTCAGC 
ACAGAAGGTC AGCGGTTTCT GAGAATAGGG CTGGCTGTGC CTCTCTTTGG GTCTATTTCA 
ACACTCAGGG CCATGGTAGC AGAGGAGGGC AAGCTCTCTC CAGACCAGGT AATTTTGACA 
GAGATGAACT CGGGTGGGTT CCAGCGCTCT TTCTCTGATG AAGATGACAT GACCAGTATA 
TCAGAGAGTG ATGTCATCTA CGCCTTCCAG GTTCCCCCTC ACTTCACCAG AGGAGGTTCC 
ATGCGGTTCT CAGATTTCAA AGGTCACAGG CTACCGCCAT CAGGGACGTT ATCAACAGAA 
TTCTTGAATC ATGGGGGCTC TTCAAAAGTC CTTCTCTTAA TCTGCAATGT TGCTGGCTCT 
GGCCAACAGG CTACAAGGTT CGGGCCACCA TTTCTGATGA GAGTAGAAAG AACTCTTTCT 
TGGGATCATC TGCAACAGAG CGTCTGCAAT AAGCTTTACG TTTTTCAAAA AACTGCAATA 
ATAAAGGTTA GTACTACTGA ACTGTTTAAA GTGCGTGTGG TGGGTGGATC AGCCTCCTGC 
AGTTATCTGT CTCCGCACGA CAGCAGACCT CTGTGCCATC CCACCGTAGA CAGAGCCCTC 
AAGCTATGTG GTCCAGGTGG TCCTTTGCAT GTGAAGCTCA TAATTGAATG GGACAACAAA 
ATTAAAGAAT GCCTCTTTGG CAATATTCAA GAGGAGGTGA TTGAGGATGC AGAGAGTGTG 
AGAATCCAGC AGCAGAATCA TGTACAACAG CACAGCTGCA CACTGGATGA CTGTTTTCAG 
CTCTACACCA AAGAGGAGCA GCTGGCACCA GACGATGCAT GGAAGTGCCC CCACTGTAAG 
CAAATGCAGC AGGGAATGGT GAAGATGAGT TTATGGACAC TGCCCGACAT CCTCATTCTG 
CACCTGAAGC GTTTTCGACA GGTGGGCAAA CGGCGAAACA AGCTCTCCAC CCTCATTCGC 
TTTCCTGTAA GTGCTCTGGA TATGACCCCC CATGTGGTGA AACGCAGTCA AAGCATGAAG 
AATCTGGCCC CGTTGCCTTC AACTTGGAAG CATGGAGAAA CTGACTTTCT TTATGACTTG 
TATGCAGTTT GCAACCACCA CGGAGGCATG CATGGAGGCC ATTACACAGC CTATTGCAGG 
AACTCCGTGG ACGGCCACTG GTACAGTTAT GATGACAGCA GTGTGGAGAT TGTGCCAGAG 
GAGGAACTGT GCACACGAGG GGCTTATATC CTGTTCTACC AGAGGAGAAA TGTCATCCCA 
CCCTGGTCTG CCAACAGCTC GGTCAGAGGT TCCACCAGCT CCTCGATGTC AGATCACTGG 
CTCATCCGTT TGAATGGTGA CAGTAAAAGA GGAAGTATGG TTTCACGATC TTCTACAACA 
TGCCCTTCTT CCATCGCTGA CTCCCCAGAG TCACCAGTTT TTAAAAATGA ACCCTCAATT 
GAGGAAAGAG GTGGGTTTGA AAGCAGACCT TTTGTGCGAG GAATCCAAGG TCGTAGTGTA 
AGCATGAGAA GTCCCACCAA GACTAAAGTG CTCCCACTGC GATGGTCTTT TGGAAATAAA 
GACCGCCACA AACCTGTGCC GGCTTCTGAG CAGTCTCCTG CAGAACTGGT GGAGTATTTA 
GAGTCTGGGA GGAGACCAAG ATGTACCAAA GACCCTATTG TTTCCCTAAT GACTCGACCT 
TCAGACAAAG ACAACGATGC AAAAAAAACA GCCAAATCTT CCAGCCACAG TGGCCTCAGA 
CCTCCAGAAT ATCTGAAAAT ACCACAGGGT CAAGAAAGAG GTAGCTCAGA GAAAGCAATG 
GGCCAACAAC CTGCCAGCAG CAGTAGATCA AGAGAAGAAG GGACCTTGAC CAGACGGAGC 
AGCAGAAAAT CTAAACCGGA CCAATCACAC TCCAGGAAGA GCTCTGGAGC AGGGATCCAG 
TCCAGTTGTA GTACTCCAAG TGGCTGGGAA ACAGCACAGA AGCATGAGTC GAGTGACAAG 
CGTGACCAAT CAGAGGATAA ACATAAAAAA GGGCAGGAGA CTAAACGTCA TGATGTCTTC 
GCTTTTCTCA AAGGTGGCTT TATCAAGAAG GATTCCCTTC GGAAGTCTCG GGATAATGAT 
TCTGTCAAAA AGGCTGATTC AGGGGTGAAG AGCCCATCTG GAGTTTCCCT CTCCAACGGG 
ACACATAGTA GAAATCCAGA AGGTAATGTC AGTACTATGA ACCATGGTAG CAGGCACGGA 
AGTGAACTGG CAAATGGCAA GGTGAGTCGT GGTTCCTCAG ACATCAAACG CTCACAAAGC 
TCATCCAATA TTCAAAGCAA AACTGATCTA GGCTTAAGAA GAACAGCATC TTTACACAAA 
AACGGCACAT CGGTGGCCCA ACCACAGAGT GTGACCGGGG ACAGGGGCTC TCAGAGCACT 
TTACAACGCA CACGCTACAG CACAAACTCA CTGGGCAGAA AGAAGGCAGT GCCTGAATCA 
AGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP43A ( XM_009302356.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300631.1 Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44217
Clone ID Related Accession (Same CDS sequence)	XM_009302356.2 , XM_009302356.3
Accession Version	XM_009302356.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 43 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGTGT CTTTAGGTGG GTTTGAAAGC 
AGACCTTTTG TGCGAGGAAT CCAAGGTCGT AGTGTAAGCA TGAGAAGTCC CACCAAGAGT 
AAAGTGCTCC CACTGCGATG GTCTTTTGGA AATAAAGACC GCCACAAACC TGTGCCGGCT 
TCTGAGCAGT CTCCTGCAGA ACTGGTGGAG TATTTAGAGT CTGGGAGGAG ACCAAGATGT 
ACCAAAGACC CTATTGTTTC CCTAATGACT CGACCTTCAG ACAAAGACAA CGATGCAAAA 
AAAACAGCCA AATCTTCCAG CCACAGTGGC CTCAGACCTC CAGAATATCT GAAAATACCA 
CAGGGTCAAG AAAGAAGTAG CTCAGAGAAA GCAATGGGCC AACAACCTGC CAGCAGCAGT 
AGATCAAGAG AAGAAGGGAC CTTGACCAGA CGGAGCAGCA GAAAATCTAA ACCGGACCAA 
TCACACTCCA GGAAGAGCTC TGGAGCAGGG ATCCAGTCCA GTTGTAGTAC TCCAAGTGGC 
TGGGAAACAG CACAGAAGCA TGAGTCGAGT GACAAGCGTG ACCAATCAGA GGATAAACAT 
AAAAAAGGGC AGGAGACTAA ACGTCATGAT GTCTTCGCTT TTCTCAAAGG TGGCTTTATC 
AAGAAGGATT CCCTTCGGAA GTCTCGGGAT AATGATTCTG TCAAAAAGGC TGATTCAGGG 
GTGAAGAGCC CATCTGGAGT TTCCCTCTCC AACGGGACAC ATAATAAAAA TCCAGAAGGT 
AAAGTCAGTA CTATGAACCA TGGTAGCAGG CACGGAAGTG AACTGGCAAA TGGCAAGGTG 
AGTCGTGGTT CCTCAGACAT CAAACGCTCA CAAAGCTCAT CCAATATTCA AAGCAAAACT 
GATCTAGGCT TAAGAAGAAC AGCATCTTTA CACAGAAACG GCACATCGGT GGCCCAACCA 
CAGAGCGTGA CAGGGGACAG GGGCTCTCAG AGCACTTTAC AACGCACACG CTACAGCACA 
AACTCACTGG GCAGAAAGAA GGCAGTGCCT GAATCAAGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300631.1
CDS	398..3862
Translation	MDSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQPDAAAEDDDGGFGDTASVHNSGNYRESSARKSSSYGSVKERLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMMKSEEVRGEVTDKLASLVRALWTNDYTPQLSVDFKSVVAKYGSQFKGNAQHDALEFLLWLLDCVHEDANLSTNNNQSRNSSSSKGSVGLEESSCPGPANSQQPGTRHSFVQEHFQAQYRSSLTCPHCLKQSNTFDPFLCISLPIPLRQTRALNVTLVFSTEGQRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQVILTEMNSGGFQRSFSDEDDMTSISESDVIYAFQVPPHFTRGGSMRFSGYHHSLPSSPYTADFKGHRLPPSGTLSTEFLNHGGSSKVLLLICNVAGSGQQATRFGPPFLMRVERTLSWDHLQQSVCNKLYVFQKTAIIKTTELFKVRVVGGSASCSYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECLFGNIQEEVIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMVKMSLWTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSMKNLAPLPSTWKHGETDFLYDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSSVEIVPEEELCTRGAYILFYQRRNVIPPWSANSSVRGSTSSSMSDHWLIRLNGDSKRGSMVSRSSTTCPSSIADSPESPVFKNEPSIEERVSLGGFESRPFVRGIQGRSVSMRSPTKSKVLPLRWSFGNKDRHKPVPASEQSPAELVEYLESGRRPRCTKDPIVSLMTRPSDKDNDAKKTAKSSSHSGLRPPEYLKIPQGQERSSSEKAMGQQPASSSRSREEGTLTRRSSRKSKPDQSHSRKSSGAGIQSSCSTPSGWETAQKHESSDKRDQSEDKHKKGQETKRHDVFAFLKGGFIKKDSLRKSRDNDSVKKADSGVKSPSGVSLSNGTHNKNPEGKVSTMNHGSRHGSELANGKVSRGSSDIKRSQSSSNIQSKTDLGLRRTASLHRNGTSVAQPQSVTGDRGSQSTLQRTRYSTNSLGRKKAVPESSF

Target ORF information:

RefSeq Version	XM_009302356.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302356.2

ORF Insert Sequence:

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGTGT CTTTAGGTGG GTTTGAAAGC 
AGACCTTTTG TGCGAGGAAT CCAAGGTCGT AGTGTAAGCA TGAGAAGTCC CACCAAGAGT 
AAAGTGCTCC CACTGCGATG GTCTTTTGGA AATAAAGACC GCCACAAACC TGTGCCGGCT 
TCTGAGCAGT CTCCTGCAGA ACTGGTGGAG TATTTAGAGT CTGGGAGGAG ACCAAGATGT 
ACCAAAGACC CTATTGTTTC CCTAATGACT CGACCTTCAG ACAAAGACAA CGATGCAAAA 
AAAACAGCCA AATCTTCCAG CCACAGTGGC CTCAGACCTC CAGAATATCT GAAAATACCA 
CAGGGTCAAG AAAGAAGTAG CTCAGAGAAA GCAATGGGCC AACAACCTGC CAGCAGCAGT 
AGATCAAGAG AAGAAGGGAC CTTGACCAGA CGGAGCAGCA GAAAATCTAA ACCGGACCAA 
TCACACTCCA GGAAGAGCTC TGGAGCAGGG ATCCAGTCCA GTTGTAGTAC TCCAAGTGGC 
TGGGAAACAG CACAGAAGCA TGAGTCGAGT GACAAGCGTG ACCAATCAGA GGATAAACAT 
AAAAAAGGGC AGGAGACTAA ACGTCATGAT GTCTTCGCTT TTCTCAAAGG TGGCTTTATC 
AAGAAGGATT CCCTTCGGAA GTCTCGGGAT AATGATTCTG TCAAAAAGGC TGATTCAGGG 
GTGAAGAGCC CATCTGGAGT TTCCCTCTCC AACGGGACAC ATAATAAAAA TCCAGAAGGT 
AAAGTCAGTA CTATGAACCA TGGTAGCAGG CACGGAAGTG AACTGGCAAA TGGCAAGGTG 
AGTCGTGGTT CCTCAGACAT CAAACGCTCA CAAAGCTCAT CCAATATTCA AAGCAAAACT 
GATCTAGGCT TAAGAAGAAC AGCATCTTTA CACAGAAACG GCACATCGGT GGCCCAACCA 
CAGAGCGTGA CAGGGGACAG GGGCTCTCAG AGCACTTTAC AACGCACACG CTACAGCACA 
AACTCACTGG GCAGAAAGAA GGCAGTGCCT GAATCAAGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP43A ( XM_009302356.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300631.1 Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.

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CloneID	ODa44217
Clone ID Related Accession (Same CDS sequence)	XM_009302356.2 , XM_009302356.3
Accession Version	XM_009302356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 43 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGTGT CTTTAGGTGG GTTTGAAAGC 
AGACCTTTTG TGCGAGGAAT CCAAGGTCGT AGTGTAAGCA TGAGAAGTCC CACCAAGAGT 
AAAGTGCTCC CACTGCGATG GTCTTTTGGA AATAAAGACC GCCACAAACC TGTGCCGGCT 
TCTGAGCAGT CTCCTGCAGA ACTGGTGGAG TATTTAGAGT CTGGGAGGAG ACCAAGATGT 
ACCAAAGACC CTATTGTTTC CCTAATGACT CGACCTTCAG ACAAAGACAA CGATGCAAAA 
AAAACAGCCA AATCTTCCAG CCACAGTGGC CTCAGACCTC CAGAATATCT GAAAATACCA 
CAGGGTCAAG AAAGAAGTAG CTCAGAGAAA GCAATGGGCC AACAACCTGC CAGCAGCAGT 
AGATCAAGAG AAGAAGGGAC CTTGACCAGA CGGAGCAGCA GAAAATCTAA ACCGGACCAA 
TCACACTCCA GGAAGAGCTC TGGAGCAGGG ATCCAGTCCA GTTGTAGTAC TCCAAGTGGC 
TGGGAAACAG CACAGAAGCA TGAGTCGAGT GACAAGCGTG ACCAATCAGA GGATAAACAT 
AAAAAAGGGC AGGAGACTAA ACGTCATGAT GTCTTCGCTT TTCTCAAAGG TGGCTTTATC 
AAGAAGGATT CCCTTCGGAA GTCTCGGGAT AATGATTCTG TCAAAAAGGC TGATTCAGGG 
GTGAAGAGCC CATCTGGAGT TTCCCTCTCC AACGGGACAC ATAATAAAAA TCCAGAAGGT 
AAAGTCAGTA CTATGAACCA TGGTAGCAGG CACGGAAGTG AACTGGCAAA TGGCAAGGTG 
AGTCGTGGTT CCTCAGACAT CAAACGCTCA CAAAGCTCAT CCAATATTCA AAGCAAAACT 
GATCTAGGCT TAAGAAGAAC AGCATCTTTA CACAGAAACG GCACATCGGT GGCCCAACCA 
CAGAGCGTGA CAGGGGACAG GGGCTCTCAG AGCACTTTAC AACGCACACG CTACAGCACA 
AACTCACTGG GCAGAAAGAA GGCAGTGCCT GAATCAAGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300631.1
CDS	576..4040
Translation	MDSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQPDAAAEDDDGGFGDTASVHNSGNYRESSARKSSSYGSVKERLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMMKSEEVRGEVTDKLASLVRALWTNDYTPQLSVDFKSVVAKYGSQFKGNAQHDALEFLLWLLDCVHEDANLSTNNNQSRNSSSSKGSVGLEESSCPGPANSQQPGTRHSFVQEHFQAQYRSSLTCPHCLKQSNTFDPFLCISLPIPLRQTRALNVTLVFSTEGQRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQVILTEMNSGGFQRSFSDEDDMTSISESDVIYAFQVPPHFTRGGSMRFSGYHHSLPSSPYTADFKGHRLPPSGTLSTEFLNHGGSSKVLLLICNVAGSGQQATRFGPPFLMRVERTLSWDHLQQSVCNKLYVFQKTAIIKTTELFKVRVVGGSASCSYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECLFGNIQEEVIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMVKMSLWTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSMKNLAPLPSTWKHGETDFLYDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSSVEIVPEEELCTRGAYILFYQRRNVIPPWSANSSVRGSTSSSMSDHWLIRLNGDSKRGSMVSRSSTTCPSSIADSPESPVFKNEPSIEERVSLGGFESRPFVRGIQGRSVSMRSPTKSKVLPLRWSFGNKDRHKPVPASEQSPAELVEYLESGRRPRCTKDPIVSLMTRPSDKDNDAKKTAKSSSHSGLRPPEYLKIPQGQERSSSEKAMGQQPASSSRSREEGTLTRRSSRKSKPDQSHSRKSSGAGIQSSCSTPSGWETAQKHESSDKRDQSEDKHKKGQETKRHDVFAFLKGGFIKKDSLRKSRDNDSVKKADSGVKSPSGVSLSNGTHNKNPEGKVSTMNHGSRHGSELANGKVSRGSSDIKRSQSSSNIQSKTDLGLRRTASLHRNGTSVAQPQSVTGDRGSQSTLQRTRYSTNSLGRKKAVPESSF

Target ORF information:

RefSeq Version	XM_009302356.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302356.3

ORF Insert Sequence:

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGTGT CTTTAGGTGG GTTTGAAAGC 
AGACCTTTTG TGCGAGGAAT CCAAGGTCGT AGTGTAAGCA TGAGAAGTCC CACCAAGAGT 
AAAGTGCTCC CACTGCGATG GTCTTTTGGA AATAAAGACC GCCACAAACC TGTGCCGGCT 
TCTGAGCAGT CTCCTGCAGA ACTGGTGGAG TATTTAGAGT CTGGGAGGAG ACCAAGATGT 
ACCAAAGACC CTATTGTTTC CCTAATGACT CGACCTTCAG ACAAAGACAA CGATGCAAAA 
AAAACAGCCA AATCTTCCAG CCACAGTGGC CTCAGACCTC CAGAATATCT GAAAATACCA 
CAGGGTCAAG AAAGAAGTAG CTCAGAGAAA GCAATGGGCC AACAACCTGC CAGCAGCAGT 
AGATCAAGAG AAGAAGGGAC CTTGACCAGA CGGAGCAGCA GAAAATCTAA ACCGGACCAA 
TCACACTCCA GGAAGAGCTC TGGAGCAGGG ATCCAGTCCA GTTGTAGTAC TCCAAGTGGC 
TGGGAAACAG CACAGAAGCA TGAGTCGAGT GACAAGCGTG ACCAATCAGA GGATAAACAT 
AAAAAAGGGC AGGAGACTAA ACGTCATGAT GTCTTCGCTT TTCTCAAAGG TGGCTTTATC 
AAGAAGGATT CCCTTCGGAA GTCTCGGGAT AATGATTCTG TCAAAAAGGC TGATTCAGGG 
GTGAAGAGCC CATCTGGAGT TTCCCTCTCC AACGGGACAC ATAATAAAAA TCCAGAAGGT 
AAAGTCAGTA CTATGAACCA TGGTAGCAGG CACGGAAGTG AACTGGCAAA TGGCAAGGTG 
AGTCGTGGTT CCTCAGACAT CAAACGCTCA CAAAGCTCAT CCAATATTCA AAGCAAAACT 
GATCTAGGCT TAAGAAGAAC AGCATCTTTA CACAGAAACG GCACATCGGT GGCCCAACCA 
CAGAGCGTGA CAGGGGACAG GGGCTCTCAG AGCACTTTAC AACGCACACG CTACAGCACA 
AACTCACTGG GCAGAAAGAA GGCAGTGCCT GAATCAAGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP43A ( XM_009302357.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300632.1 Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44218
Clone ID Related Accession (Same CDS sequence)	XM_009302357.3 , XM_009302357.2
Accession Version	XM_009302357.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 43 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGGTG GGTTTGAAAG CAGACCTTTT 
GTGCGAGGAA TCCAAGGTCG TAGTGTAAGC ATGAGAAGTC CCACCAAGAG TAAAGTGCTC 
CCACTGCGAT GGTCTTTTGG AAATAAAGAC CGCCACAAAC CTGTGCCGGC TTCTGAGCAG 
TCTCCTGCAG AACTGGTGGA GTATTTAGAG TCTGGGAGGA GACCAAGATG TACCAAAGAC 
CCTATTGTTT CCCTAATGAC TCGACCTTCA GACAAAGACA ACGATGCAAA AAAAACAGCC 
AAATCTTCCA GCCACAGTGG CCTCAGACCT CCAGAATATC TGAAAATACC ACAGGGTCAA 
GAAAGAAGTA GCTCAGAGAA AGCAATGGGC CAACAACCTG CCAGCAGCAG TAGATCAAGA 
GAAGAAGGGA CCTTGACCAG ACGGAGCAGC AGAAAATCTA AACCGGACCA ATCACACTCC 
AGGAAGAGCT CTGGAGCAGG GATCCAGTCC AGTTGTAGTA CTCCAAGTGG CTGGGAAACA 
GCACAGAAGC ATGAGTCGAG TGACAAGCGT GACCAATCAG AGGATAAACA TAAAAAAGGG 
CAGGAGACTA AACGTCATGA TGTCTTCGCT TTTCTCAAAG GTGGCTTTAT CAAGAAGGAT 
TCCCTTCGGA AGTCTCGGGA TAATGATTCT GTCAAAAAGG CTGATTCAGG GGTGAAGAGC 
CCATCTGGAG TTTCCCTCTC CAACGGGACA CATAATAAAA ATCCAGAAGG TAAAGTCAGT 
ACTATGAACC ATGGTAGCAG GCACGGAAGT GAACTGGCAA ATGGCAAGGT GAGTCGTGGT 
TCCTCAGACA TCAAACGCTC ACAAAGCTCA TCCAATATTC AAAGCAAAAC TGATCTAGGC 
TTAAGAAGAA CAGCATCTTT ACACAGAAAC GGCACATCGG TGGCCCAACC ACAGAGCGTG 
ACAGGGGACA GGGGCTCTCA GAGCACTTTA CAACGCACAC GCTACAGCAC AAACTCACTG 
GGCAGAAAGA AGGCAGTGCC TGAATCAAGT TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300632.1
CDS	577..4032
Translation	MDSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQPDAAAEDDDGGFGDTASVHNSGNYRESSARKSSSYGSVKERLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMMKSEEVRGEVTDKLASLVRALWTNDYTPQLSVDFKSVVAKYGSQFKGNAQHDALEFLLWLLDCVHEDANLSTNNNQSRNSSSSKGSVGLEESSCPGPANSQQPGTRHSFVQEHFQAQYRSSLTCPHCLKQSNTFDPFLCISLPIPLRQTRALNVTLVFSTEGQRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQVILTEMNSGGFQRSFSDEDDMTSISESDVIYAFQVPPHFTRGGSMRFSGYHHSLPSSPYTADFKGHRLPPSGTLSTEFLNHGGSSKVLLLICNVAGSGQQATRFGPPFLMRVERTLSWDHLQQSVCNKLYVFQKTAIIKTTELFKVRVVGGSASCSYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECLFGNIQEEVIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMVKMSLWTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSMKNLAPLPSTWKHGETDFLYDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSSVEIVPEEELCTRGAYILFYQRRNVIPPWSANSSVRGSTSSSMSDHWLIRLNGDSKRGSMVSRSSTTCPSSIADSPESPVFKNEPSIEERGGFESRPFVRGIQGRSVSMRSPTKSKVLPLRWSFGNKDRHKPVPASEQSPAELVEYLESGRRPRCTKDPIVSLMTRPSDKDNDAKKTAKSSSHSGLRPPEYLKIPQGQERSSSEKAMGQQPASSSRSREEGTLTRRSSRKSKPDQSHSRKSSGAGIQSSCSTPSGWETAQKHESSDKRDQSEDKHKKGQETKRHDVFAFLKGGFIKKDSLRKSRDNDSVKKADSGVKSPSGVSLSNGTHNKNPEGKVSTMNHGSRHGSELANGKVSRGSSDIKRSQSSSNIQSKTDLGLRRTASLHRNGTSVAQPQSVTGDRGSQSTLQRTRYSTNSLGRKKAVPESSF

Target ORF information:

RefSeq Version	XM_009302357.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302357.3

ORF Insert Sequence:

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGGTG GGTTTGAAAG CAGACCTTTT 
GTGCGAGGAA TCCAAGGTCG TAGTGTAAGC ATGAGAAGTC CCACCAAGAG TAAAGTGCTC 
CCACTGCGAT GGTCTTTTGG AAATAAAGAC CGCCACAAAC CTGTGCCGGC TTCTGAGCAG 
TCTCCTGCAG AACTGGTGGA GTATTTAGAG TCTGGGAGGA GACCAAGATG TACCAAAGAC 
CCTATTGTTT CCCTAATGAC TCGACCTTCA GACAAAGACA ACGATGCAAA AAAAACAGCC 
AAATCTTCCA GCCACAGTGG CCTCAGACCT CCAGAATATC TGAAAATACC ACAGGGTCAA 
GAAAGAAGTA GCTCAGAGAA AGCAATGGGC CAACAACCTG CCAGCAGCAG TAGATCAAGA 
GAAGAAGGGA CCTTGACCAG ACGGAGCAGC AGAAAATCTA AACCGGACCA ATCACACTCC 
AGGAAGAGCT CTGGAGCAGG GATCCAGTCC AGTTGTAGTA CTCCAAGTGG CTGGGAAACA 
GCACAGAAGC ATGAGTCGAG TGACAAGCGT GACCAATCAG AGGATAAACA TAAAAAAGGG 
CAGGAGACTA AACGTCATGA TGTCTTCGCT TTTCTCAAAG GTGGCTTTAT CAAGAAGGAT 
TCCCTTCGGA AGTCTCGGGA TAATGATTCT GTCAAAAAGG CTGATTCAGG GGTGAAGAGC 
CCATCTGGAG TTTCCCTCTC CAACGGGACA CATAATAAAA ATCCAGAAGG TAAAGTCAGT 
ACTATGAACC ATGGTAGCAG GCACGGAAGT GAACTGGCAA ATGGCAAGGT GAGTCGTGGT 
TCCTCAGACA TCAAACGCTC ACAAAGCTCA TCCAATATTC AAAGCAAAAC TGATCTAGGC 
TTAAGAAGAA CAGCATCTTT ACACAGAAAC GGCACATCGG TGGCCCAACC ACAGAGCGTG 
ACAGGGGACA GGGGCTCTCA GAGCACTTTA CAACGCACAC GCTACAGCAC AAACTCACTG 
GGCAGAAAGA AGGCAGTGCC TGAATCAAGT TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP43A ( XM_009302357.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300632.1 Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.

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CloneID	ODa44218
Clone ID Related Accession (Same CDS sequence)	XM_009302357.3 , XM_009302357.2
Accession Version	XM_009302357.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 43 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGGTG GGTTTGAAAG CAGACCTTTT 
GTGCGAGGAA TCCAAGGTCG TAGTGTAAGC ATGAGAAGTC CCACCAAGAG TAAAGTGCTC 
CCACTGCGAT GGTCTTTTGG AAATAAAGAC CGCCACAAAC CTGTGCCGGC TTCTGAGCAG 
TCTCCTGCAG AACTGGTGGA GTATTTAGAG TCTGGGAGGA GACCAAGATG TACCAAAGAC 
CCTATTGTTT CCCTAATGAC TCGACCTTCA GACAAAGACA ACGATGCAAA AAAAACAGCC 
AAATCTTCCA GCCACAGTGG CCTCAGACCT CCAGAATATC TGAAAATACC ACAGGGTCAA 
GAAAGAAGTA GCTCAGAGAA AGCAATGGGC CAACAACCTG CCAGCAGCAG TAGATCAAGA 
GAAGAAGGGA CCTTGACCAG ACGGAGCAGC AGAAAATCTA AACCGGACCA ATCACACTCC 
AGGAAGAGCT CTGGAGCAGG GATCCAGTCC AGTTGTAGTA CTCCAAGTGG CTGGGAAACA 
GCACAGAAGC ATGAGTCGAG TGACAAGCGT GACCAATCAG AGGATAAACA TAAAAAAGGG 
CAGGAGACTA AACGTCATGA TGTCTTCGCT TTTCTCAAAG GTGGCTTTAT CAAGAAGGAT 
TCCCTTCGGA AGTCTCGGGA TAATGATTCT GTCAAAAAGG CTGATTCAGG GGTGAAGAGC 
CCATCTGGAG TTTCCCTCTC CAACGGGACA CATAATAAAA ATCCAGAAGG TAAAGTCAGT 
ACTATGAACC ATGGTAGCAG GCACGGAAGT GAACTGGCAA ATGGCAAGGT GAGTCGTGGT 
TCCTCAGACA TCAAACGCTC ACAAAGCTCA TCCAATATTC AAAGCAAAAC TGATCTAGGC 
TTAAGAAGAA CAGCATCTTT ACACAGAAAC GGCACATCGG TGGCCCAACC ACAGAGCGTG 
ACAGGGGACA GGGGCTCTCA GAGCACTTTA CAACGCACAC GCTACAGCAC AAACTCACTG 
GGCAGAAAGA AGGCAGTGCC TGAATCAAGT TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300632.1
CDS	398..3853
Translation	MDSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQPDAAAEDDDGGFGDTASVHNSGNYRESSARKSSSYGSVKERLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMMKSEEVRGEVTDKLASLVRALWTNDYTPQLSVDFKSVVAKYGSQFKGNAQHDALEFLLWLLDCVHEDANLSTNNNQSRNSSSSKGSVGLEESSCPGPANSQQPGTRHSFVQEHFQAQYRSSLTCPHCLKQSNTFDPFLCISLPIPLRQTRALNVTLVFSTEGQRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQVILTEMNSGGFQRSFSDEDDMTSISESDVIYAFQVPPHFTRGGSMRFSGYHHSLPSSPYTADFKGHRLPPSGTLSTEFLNHGGSSKVLLLICNVAGSGQQATRFGPPFLMRVERTLSWDHLQQSVCNKLYVFQKTAIIKTTELFKVRVVGGSASCSYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECLFGNIQEEVIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMVKMSLWTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSMKNLAPLPSTWKHGETDFLYDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSSVEIVPEEELCTRGAYILFYQRRNVIPPWSANSSVRGSTSSSMSDHWLIRLNGDSKRGSMVSRSSTTCPSSIADSPESPVFKNEPSIEERGGFESRPFVRGIQGRSVSMRSPTKSKVLPLRWSFGNKDRHKPVPASEQSPAELVEYLESGRRPRCTKDPIVSLMTRPSDKDNDAKKTAKSSSHSGLRPPEYLKIPQGQERSSSEKAMGQQPASSSRSREEGTLTRRSSRKSKPDQSHSRKSSGAGIQSSCSTPSGWETAQKHESSDKRDQSEDKHKKGQETKRHDVFAFLKGGFIKKDSLRKSRDNDSVKKADSGVKSPSGVSLSNGTHNKNPEGKVSTMNHGSRHGSELANGKVSRGSSDIKRSQSSSNIQSKTDLGLRRTASLHRNGTSVAQPQSVTGDRGSQSTLQRTRYSTNSLGRKKAVPESSF

Target ORF information:

RefSeq Version	XM_009302357.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 43a (usp43a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302357.2

ORF Insert Sequence:

ATGGATTCGC TGAGGGAAAC CAGGAAACAA ACAATCAAAG CTAAATCGGC GAAGCGCAAG 
AACAAAACGC ACAAGTCCCA AAAGCTGTTC CGAAGGAAAT CGTTAAAATC GCTGGGAAAT 
TTTATGAGCA GGATTGTCAA AACTTTGGGC ACTTTGGCAC ACTTGGGGGA CGCGGATCAG 
CCGGACGCAG CAGCGGAGGA TGATGATGGT GGATTTGGAG ACACGGCGAG TGTCCACAAC 
TCTGGGAACT ACAGAGAGAG CTCGGCGAGG AAAAGCTCTT CTTACGGGTC TGTGAAAGAA 
AGACTGTCCT GGTGTTACGG AGAGAAAACA CCGGGAGTTC AGGGTCTGAA GAATCACGGA 
AACACCTGTT TTATGAACGC TGTGGTGCAG TGTCTGAGTA ACACAGAACT TCTGGCTGAA 
TATCTGGGTC TGGAGCAGTA TAAATATGAA TTAAATGAGC GGAGGATCAA CGGGATGATG 
AAGAGTGAGG AGGTCAGAGG GGAAGTCACG GACAAGCTGG CATCGTTAGT AAGGGCGCTC 
TGGACAAACG ACTACACGCC TCAGCTGTCG GTGGATTTTA AGAGTGTTGT GGCCAAGTAT 
GGCTCTCAGT TCAAAGGAAA CGCTCAACAT GATGCTTTAG AGTTTCTGCT CTGGCTTCTG 
GATTGTGTGC ATGAGGACGC TAACCTCTCC ACCAACAACA ACCAAAGCAG AAATAGTTCA 
TCCAGCAAGG GTTCTGTGGG ATTGGAGGAG AGTTCATGCC CTGGCCCAGC AAACTCACAG 
CAGCCCGGAA CTCGACACAG TTTTGTACAG GAGCACTTCC AAGCCCAGTA CAGGTCATCC 
TTGACATGCC CGCATTGCCT TAAACAGAGC AACACTTTTG ACCCTTTCCT GTGTATTTCC 
CTTCCAATCC CGCTGCGACA GACCAGGGCC CTGAATGTGA CTCTGGTGTT CAGCACAGAA 
GGTCAGCGGT TTCTGAGAAT AGGGCTGGCT GTGCCTCTCT TTGGGTCTAT TTCAACACTC 
AGGGCCATGG TAGCAGAGGA GGGCAAGCTC TCTCCAGACC AGGTAATTTT GACAGAGATG 
AACTCGGGTG GGTTCCAGCG CTCTTTCTCT GATGAAGATG ACATGACCAG TATATCAGAG 
AGTGATGTCA TCTACGCCTT CCAGGTTCCC CCTCACTTCA CCAGAGGAGG TTCCATGCGG 
TTCTCAGGGT ATCATCACTC CCTTCCCTCC TCCCCATACA CTGCAGATTT CAAAGGTCAC 
AGGCTACCGC CATCAGGGAC GTTATCAACA GAATTCTTGA ATCATGGGGG CTCTTCAAAA 
GTCCTTCTCT TAATCTGCAA TGTTGCTGGC TCTGGCCAAC AGGCTACAAG GTTCGGGCCA 
CCATTTCTGA TGAGAGTAGA AAGGACTCTT TCTTGGGATC ATCTGCAACA GAGTGTCTGC 
AATAAGCTTT ACGTTTTTCA AAAAACTGCA ATAATAAAGA CTACTGAACT GTTTAAAGTG 
CGTGTGGTGG GTGGATCAGC CTCCTGCAGT TATCTGTCTC CGCACGACAG CAGACCTCTG 
TGCCATCCCA CCGTAGACAG AGCCCTCAAG TTATGTGGTC CAGGTGGTCC TTTGCATGTG 
AAGCTCATAA TTGAATGGGA CAACAAAATT AAAGAATGCC TCTTTGGCAA TATTCAAGAG 
GAGGTGATTG AGGATGCAGA GAGTGTGAGA ATCCAGCAGC AGAATCATGT ACAACAGCAC 
AGCTGCACAC TGGATGACTG TTTTCAGCTC TACACCAAAG AGGAGCAGCT GGCACCAGAC 
GATGCATGGA AGTGCCCCCA CTGTAAGCAA ATGCAGCAGG GAATGGTGAA GATGAGTTTA 
TGGACACTGC CCGACATCCT CATTCTGCAC CTGAAGCGTT TTCGACAGGT GGGCAAACGG 
CGAAACAAGC TCTCCACCCT CATTCGCTTT CCTGTAAGTG CTCTGGATAT GACCCCCCAT 
GTGGTGAAAC GCAGTCAAAG CATGAAGAAT CTGGCCCCGT TGCCTTCAAC TTGGAAGCAT 
GGAGAAACTG ACTTTCTTTA TGACTTGTAT GCAGTTTGCA ACCACCACGG AGGCATGCAT 
GGAGGCCATT ACACAGCCTA TTGCAGGAAC TCCGTGGACG GCCACTGGTA CAGTTATGAT 
GACAGCAGTG TGGAGATTGT GCCAGAGGAG GAACTGTGCA CACGAGGGGC TTATATCCTG 
TTCTACCAGA GGAGAAATGT CATCCCACCC TGGTCTGCCA ACAGCTCGGT CAGAGGTTCC 
ACCAGCTCCT CGATGTCAGA TCACTGGCTC ATCCGTTTGA ATGGTGACAG TAAAAGAGGA 
AGTATGGTTT CACGGTCTTC TACAACATGC CCTTCTTCCA TCGCTGACTC CCCAGAGTCA 
CCAGTTTTTA AAAATGAACC CTCAATTGAG GAAAGAGGTG GGTTTGAAAG CAGACCTTTT 
GTGCGAGGAA TCCAAGGTCG TAGTGTAAGC ATGAGAAGTC CCACCAAGAG TAAAGTGCTC 
CCACTGCGAT GGTCTTTTGG AAATAAAGAC CGCCACAAAC CTGTGCCGGC TTCTGAGCAG 
TCTCCTGCAG AACTGGTGGA GTATTTAGAG TCTGGGAGGA GACCAAGATG TACCAAAGAC 
CCTATTGTTT CCCTAATGAC TCGACCTTCA GACAAAGACA ACGATGCAAA AAAAACAGCC 
AAATCTTCCA GCCACAGTGG CCTCAGACCT CCAGAATATC TGAAAATACC ACAGGGTCAA 
GAAAGAAGTA GCTCAGAGAA AGCAATGGGC CAACAACCTG CCAGCAGCAG TAGATCAAGA 
GAAGAAGGGA CCTTGACCAG ACGGAGCAGC AGAAAATCTA AACCGGACCA ATCACACTCC 
AGGAAGAGCT CTGGAGCAGG GATCCAGTCC AGTTGTAGTA CTCCAAGTGG CTGGGAAACA 
GCACAGAAGC ATGAGTCGAG TGACAAGCGT GACCAATCAG AGGATAAACA TAAAAAAGGG 
CAGGAGACTA AACGTCATGA TGTCTTCGCT TTTCTCAAAG GTGGCTTTAT CAAGAAGGAT 
TCCCTTCGGA AGTCTCGGGA TAATGATTCT GTCAAAAAGG CTGATTCAGG GGTGAAGAGC 
CCATCTGGAG TTTCCCTCTC CAACGGGACA CATAATAAAA ATCCAGAAGG TAAAGTCAGT 
ACTATGAACC ATGGTAGCAG GCACGGAAGT GAACTGGCAA ATGGCAAGGT GAGTCGTGGT 
TCCTCAGACA TCAAACGCTC ACAAAGCTCA TCCAATATTC AAAGCAAAAC TGATCTAGGC 
TTAAGAAGAA CAGCATCTTT ACACAGAAAC GGCACATCGG TGGCCCAACC ACAGAGCGTG 
ACAGGGGACA GGGGCTCTCA GAGCACTTTA CAACGCACAC GCTACAGCAC AAACTCACTG 
GGCAGAAAGA AGGCAGTGCC TGAATCAAGT TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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USP43A cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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