chia.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chia.3
Entrez Gene ID	406819
Full Name	chitinase, acidic.3
Synonyms	chioIb,wu:fd61a02,wu:fi13f01,zgc:55406,zgc:77912
General protein information	Preferred Names chitinase, acidic.3 Names chitinase, acidic.3 chia3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213213.1	NP_998378.1	chitinase, acidic.3 precursor

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_01247	XP_003714154.1
Danio rerio (zebrafish)	zgc:65788	NP_955897.2
Danio rerio (zebrafish)	chia.4	NP_956740.1
Bos taurus (cattle)	CHIA	NP_777124.1
Gallus gallus (chicken)	CHIA	NP_989760.1
Danio rerio (zebrafish)	chia.3	NP_998378.1
Homo sapiens (human)	CHIA	NP_970615.2
Xenopus tropicalis (tropical clawed frog)	chia	NP_001186489.1
Neurospora crassa	NCU03209	XP_965309.2
Macaca mulatta (Rhesus monkey)	CHIA	XP_001104487.1
Canis lupus familiaris (dog)	CHIA	XP_537030.3
Mus musculus (house mouse)	Chia1	NP_075675.2
Rattus norvegicus (Norway rat)	Chia	NP_997469.1
Danio rerio (zebrafish)	zgc:173927	NP_001103511.1

Gene Ontology
Bibliography

Related articles in PubMed

A six-months study on Black Soldier Fly (Hermetia illucens) based diets in zebrafish.
Zarantoniello M, Randazzo B, Truzzi C, Giorgini E, Marcellucci C, Vargas-Abúndez JA, Zimbelli A, Annibaldi A, Parisi G, Tulli F, Riolo P, Olivotto I
Scientific reports9(1)8598(2019 Jun)

Partial Dietary Inclusion of Hermetia illucens (Black Soldier Fly) Full-Fat Prepupae in Zebrafish Feed: Biometric, Histological, Biochemical, and Molecular Implications.
Zarantoniello M, Bruni L, Randazzo B, Vargas A, Gioacchini G, Truzzi C, Annibaldi A, Riolo P, Parisi G, Cardinaletti G, Tulli F, Olivotto I
Zebrafish15(5)519-532(2018 10)

Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.
Vargas A, Randazzo B, Riolo P, Truzzi C, Gioacchini G, Giorgini E, Loreto N, Ruschioni S, Zarantoniello M, Antonucci M, Polverini S, Cardinaletti G, Sabbatini S, Tulli F, Olivotto I
Zebrafish15(4)404-419(2018 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish.
Teng Z, Sun C, Liu S, Wang H, Zhang S
Developmental and comparative immunology46(2)489-98(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chia.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chia.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01912	NM_213213.1 Latest version!	Danio rerio chitinase, acidic.3 (chia.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chia.3 ( NM_213213.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998378.1 Danio rerio chitinase, acidic.3 (chia.3), mRNA.

Price & Availability↑

CloneID	ODa01912
Clone ID Related Accession (Same CDS sequence)	NM_213213.1
Accession Version	NM_213213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573228800000
Organism	Danio rerio(zebrafish)
Product	chitinase, acidic.3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065893.1. On Aug 27, 2004 this sequence version replaced NM_213498.1. ##Evidence-Data-START## Transcript exon combination :: BC065893.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476796 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAGAC TTACACTTAT AGCAGGTTTG AGCTTGGTGC TCTGCCATGT CGCCTTTTCC 
ATGGAAATGG CCTGCTACTT CACCAACTGG TCCCAATATA GACCTGGAAT TGGAAAATAT 
ACTCCTGCAA ATGTCGACCC TTACCTTTGC ACACACCTTA TCTATGCTTT TTCAATCATC 
AACCAAAGGA ATGAGCTTGT CACATATGAG TGGAACGATG AAACTCTATA CAAGGCCTTC 
AATGAACTCA AGAACAAAAA TCCCACTCTT AAGACCCTTT TGGCTGTTGG AGGATGGAAT 
TTTGGTTCGG CACAGTTCTC CATCATGGTG TCCAATCCTG CAAACCGTAA AACATTTATT 
CAGTCTACCA TCAAATTCCT GAGAACTCAT GGATTTGATG GACTGGATCT GGACTGGGAA 
TATCCCGGAG CAAGAGGAAG TCCACCTGAA GACAAACAAA GATTCACTCT GCTGTGCAAG 
GAACTTGTTG CAGCCTATGA GGCTGAGAGT AAAGCCACTG GCAATCCTCA GCTTATGCTG 
ACCGCTGCTG TATCAGCTGG CAAAGGCACT ATTGATGATG GATATGAGAT TGCAGAGATT 
GCCAAGTACT TGAACTTCAT CAACGTCATG ACTTACGACT TCCATGGCAC TTGGGAGCGA 
TTCACAGGAC ACAACAGCCC TCTGTACCAA GGCTCAAAGG ATGAGGGAGA CCTGATCTAC 
TTCAACACCG ACTATGCTAT GCGCTACTGG AGGGACAATG GAACCCCTGT GGAGAAACTC 
AGAATGGGCT TTGCAGCATA CGGTCGCACT TTCCGTCTGA CATCTTCAGA TACCAGCGTT 
GGAGCTCCAG CTAGTGGACC TGCTTCAGCT GGAACCTACA CTCGCGAGGC TGGATTCTGG 
TCTTACTATG AGATCTGTGG ATTCCTGGAG GGAACAACAA TTCAGTGGAT TGATGACCAG 
AAGGTGCCCT ATGCCACAAA GAACAGCGAG TGGGTTGGAT TTGACACCAA GGAGAGTTAT 
GAAACGAAGG TCCGTTATCT GAAAGACAAG AATTTTGGTG GAGCTTTCGT TTGGGCACTT 
GATCTGGATG ACTTTGCTGG ACAGTTCTGT AGTCAGGGGA ACCATCCTCT CATGGCCCAT 
CTTCGCAATC TTCTGGATAT TGAATTGCCT CCAATGCCTT CAACTACCAC TCCTAAACCT 
GGCCAAAGCA CCACAAGGCC GACCACAACC ACAACTACCA CCACTCATGC TCCAGGACCA 
GGATTCTGTA ATGGGAAGCC AGATGGACTC TATGCTCACC CTAATGACCC CAACAAATAT 
TACAGCTGTG CTGGAGGTCA TACCTTCGTG GAAAAATGTG CTGTAGGCAC CGTGTTTGAT 
GACAGCTGCA AGTGCTGTGT TTGGCCCAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998378.1
CDS	28..1443
Translation	MGRLTLIAGLSLVLCHVAFSMEMACYFTNWSQYRPGIGKYTPANVDPYLCTHLIYAFSIINQRNELVTYEWNDETLYKAFNELKNKNPTLKTLLAVGGWNFGSAQFSIMVSNPANRKTFIQSTIKFLRTHGFDGLDLDWEYPGARGSPPEDKQRFTLLCKELVAAYEAESKATGNPQLMLTAAVSAGKGTIDDGYEIAEIAKYLNFINVMTYDFHGTWERFTGHNSPLYQGSKDEGDLIYFNTDYAMRYWRDNGTPVEKLRMGFAAYGRTFRLTSSDTSVGAPASGPASAGTYTREAGFWSYYEICGFLEGTTIQWIDDQKVPYATKNSEWVGFDTKESYETKVRYLKDKNFGGAFVWALDLDDFAGQFCSQGNHPLMAHLRNLLDIELPPMPSTTTPKPGQSTTRPTTTTTTTTHAPGPGFCNGKPDGLYAHPNDPNKYYSCAGGHTFVEKCAVGTVFDDSCKCCVWPKP

Target ORF information:

RefSeq Version	NM_213213.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chitinase, acidic.3 (chia.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213213.1

ORF Insert Sequence:

ATGGGGAGAC TTACACTTAT AGCAGGTTTG AGCTTGGTGC TCTGCCATGT CGCCTTTTCC 
ATGGAAATGG CCTGCTACTT CACCAACTGG TCCCAATATA GACCTGGAAT TGGAAAATAT 
ACTCCTGCAA ATGTCGACCC TTACCTTTGC ACACACCTTA TCTATGCTTT TTCAATCATC 
AACCAAAGGA ATGAGCTTGT CACATATGAG TGGAACGATG AAACTCTATA CAAGGCCTTC 
AATGAACTCA AGAACAAAAA TCCCACTCTT AAGACCCTTT TGGCTGTTGG AGGATGGAAT 
TTTGGTTCGG CACAGTTCTC CATCATGGTG TCCAATCCTG CAAACCGTAA AACATTTATT 
CAGTCTACCA TCAAATTCCT GAGAACTCAT GGATTTGATG GACTGGATCT GGACTGGGAA 
TATCCCGGAG CAAGAGGAAG TCCACCTGAA GACAAACAAA GATTCACTCT GCTGTGCAAG 
GAACTTGTTG CAGCCTATGA GGCTGAGAGT AAAGCCACTG GCAATCCTCA GCTTATGCTG 
ACCGCTGCTG TATCAGCTGG CAAAGGCACT ATTGATGATG GATATGAGAT TGCAGAGATT 
GCCAAGTACT TGAACTTCAT CAACGTCATG ACTTACGACT TCCATGGCAC TTGGGAGCGA 
TTCACAGGAC ACAACAGCCC TCTGTACCAA GGCTCAAAGG ATGAGGGAGA CCTGATCTAC 
TTCAACACCG ACTATGCTAT GCGCTACTGG AGGGACAATG GAACCCCTGT GGAGAAACTC 
AGAATGGGCT TTGCAGCATA CGGTCGCACT TTCCGTCTGA CATCTTCAGA TACCAGCGTT 
GGAGCTCCAG CTAGTGGACC TGCTTCAGCT GGAACCTACA CTCGCGAGGC TGGATTCTGG 
TCTTACTATG AGATCTGTGG ATTCCTGGAG GGAACAACAA TTCAGTGGAT TGATGACCAG 
AAGGTGCCCT ATGCCACAAA GAACAGCGAG TGGGTTGGAT TTGACACCAA GGAGAGTTAT 
GAAACGAAGG TCCGTTATCT GAAAGACAAG AATTTTGGTG GAGCTTTCGT TTGGGCACTT 
GATCTGGATG ACTTTGCTGG ACAGTTCTGT AGTCAGGGGA ACCATCCTCT CATGGCCCAT 
CTTCGCAATC TTCTGGATAT TGAATTGCCT CCAATGCCTT CAACTACCAC TCCTAAACCT 
GGCCAAAGCA CCACAAGGCC GACCACAACC ACAACTACCA CCACTCATGC TCCAGGACCA 
GGATTCTGTA ATGGGAAGCC AGATGGACTC TATGCTCACC CTAATGACCC CAACAAATAT 
TACAGCTGTG CTGGAGGTCA TACCTTCGTG GAAAAATGTG CTGTAGGCAC CGTGTTTGAT 
GACAGCTGCA AGTGCTGTGT TTGGCCCAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A six-months study on Black Soldier Fly (Hermetia illucens) based diets in zebrafish.
Scientific reports9(1)8598(2019 Jun)
Zarantoniello M,Randazzo B,Truzzi C,Giorgini E,Marcellucci C,Vargas-Abúndez JA,Zimbelli A,Annibaldi A,Parisi G,Tulli F,Riolo P,Olivotto I

Partial Dietary Inclusion of Hermetia illucens (Black Soldier Fly) Full-Fat Prepupae in Zebrafish Feed: Biometric, Histological, Biochemical, and Molecular Implications.
Zebrafish15(5)519-532(2018 10)
Zarantoniello M,Bruni L,Randazzo B,Vargas A,Gioacchini G,Truzzi C,Annibaldi A,Riolo P,Parisi G,Cardinaletti G,Tulli F,Olivotto I

Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.
Zebrafish15(4)404-419(2018 08)
Vargas A,Randazzo B,Riolo P,Truzzi C,Gioacchini G,Giorgini E,Loreto N,Ruschioni S,Zarantoniello M,Antonucci M,Polverini S,Cardinaletti G,Sabbatini S,Tulli F,Olivotto I

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish.
Developmental and comparative immunology46(2)489-98(2014 Oct)
Teng Z,Sun C,Liu S,Wang H,Zhang S

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chia.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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