CNF03740 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNF03740
Entrez Gene ID	3258220
Full Name	phosphatidylinositol 3-kinase TOR1
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

mRNA and Protein(s)

mRNA	Protein	Name
XM_571336.2	XP_571336.1	phosphatidylinositol 3-kinase TOR1
XM_571336.1	XP_571336.1	phosphatidylinositol 3-kinase TOR1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D, Vamathevan J, Miranda M, Anderson IJ, Fraser JA, Allen JE, Bosdet IE, Brent MR, Chiu R, Doering TL, Donlin MJ, D'Souza CA, Fox DS, Grinberg V, Fu J, Fukushima M, Haas BJ, Huang JC, Janbon G, Jones SJ, Koo HL, Krzywinski MI, Kwon-Chung JK, Lengeler KB, Maiti R, Marra MA, Marra RE, Mathewson CA, Mitchell TG, Pertea M, Riggs FR, Salzberg SL, Schein JE, Shvartsbeyn A, Shin H, Shumway M, Specht CA, Suh BB, Tenney A, Utterback TR, Wickes BL, Wortman JR, Wye NH, Kronstad JW, Lodge JK, Heitman J, Davis RW, Fraser CM, Hyman RW
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNF03740 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNF03740 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCn04903	XM_571336.2 Latest version!	Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1 (CNF03740), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OCn04903	XM_571336.1	Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNF03740 ( XM_571336.2 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_571336.1 Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1 (CNF03740), mRNA.

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CloneID	OCn04903
Clone ID Related Accession (Same CDS sequence)	XM_571336.2 , XM_571336.1
Accession Version	XM_571336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523289600000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	phosphatidylinositol 3-kinase TOR1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006691). On Apr 11, 2018 this sequence version replaced XM_571336.1.

ATGTCTTCAC AATCAGACGT TCTCGACAAC ATCTTTCAGC GTCTGTCTGC CAGGTCGGAG 
GATGTCCGCG CTCAGGCCGG TCAAGATCTT GCAGAGCACG TCGTAGCTTA TACCCAGGAA 
TACCCGGGAC ATGATGCTTC GAAGGGTGTA TGGGCACAGG TCTTTCACAA GACATTCGAG 
TTTACTAGGA GCAACAATCA GCTAGAAAGA TTAGGTGCAA TTATTTCCAT CTCGCAATTG 
TTACAACTAA CGAAGGATGA CACACTGGAT CGTGCTCAAC AAAAGGTTCT ACGCCTCTAT 
GAATATCTTC GGCCTCTCAC AACATGCGGC GATTCGACCG TCATGCTTCC CGCCTCGCTC 
GTCGTTGAAG ATATGGTCCG CAATTCCCCC ACCCTTCACA CCGATACTTT CCTTGGCAAA 
GAAGTTGGAC AAGCGCTGGT AATGATTGAC GACTCACGTC AAGAAGTCGG TCGTTTCAGT 
GGTGCTCTTC TTCTCTATGC TTTCGCCCGT GCTGCCCCCG GAGTATTCCA TCAATACATC 
CCCAAGGTCT TGGAAAAGAT TTGGGTTCCC CTTCGCGACT CAAGATCTGT CGTTCGAGAA 
CGTGCGAGCA TGCTCTTATC TACCTGCCTT GACACTCTCA AAACCCGAGG AGACCGACCA 
TCAACCGACA CCTACCGCAA GATCTTTGAA GAGGCCCGTC TTGGTCTCCT CAAAGCCAGC 
TCAACAGAGT CTATCTTGGG TTCTCTCCTT GCTTTCAATT CCATGCTTCA AAATCAACAG 
CTTTCGATGG CGGAATATTA TCGCTCCATT TGCGAACTAA CCTTCAAATA CCGTGATTCG 
AAGGAAGTTT CCATCAGGAA GGCTGTCATC GCCCTCATAC CTTCTATGGC GACATATGAT 
AGCGACGACT TTGAGGCACA TTATTTGCAT AGGAGTATGG CGTATTTGCT ACAAGCGTTG 
AACAGGCCGG CGGACAGGGA CATTTCGTAT GTGGCATTGG GTCATATGGC CGTACATCTT 
GGATCGAAAA TGAAACCTTT TATTGACGAC ATTATGCGGA TCATTCGAGA TCATCTACGC 
ATGCGAGGCA AAAAAAATGC TCCATACGAA GCCCCCATCT TCCAGTGTCT TGCGATGCTC 
GCTACCTCTG TCGGCCCTAT GCTTACCCGC CAGATGCACG AAATCCTCAA CCTGATGTTC 
CCCTGGGGCC TTTCGGAACC CTTATGTACA GCCCTTCAAG CCACTGCCTC ACATATCCCT 
CCTCTATTGA GGACTATCCA AGATAGGTTG TTGGAGATGC TTTCACAGAC TTTGACCGGA 
CACAGCTATA GACCGTTAGG TGCGCCTGCC CCAAGAGGTG GAGCGCAGAT GGATCTTAAC 
CTTTTGCAAT CTACAACCAA TGCCCAGTCA GCAGACACCC TAAAACTTGC TCTTCGTCTC 
CTTGCGCGCT TTGATTTTGT CGGCCATACC CTTAGCGAGT TTGTCCGTGA CGCTGCTCTT 
CCGTATCTGG AACACGACAG TGTCGAGGTC CGACGTGAAG CCGTGTTGGC GACCACGACC 
CTGTTCATGA CGGACCCGAT CTGTCAACAG ACGAGCAGTA ATTCGGTCGA GATTGTAAAT 
GATGTGTTGA GTAAATTGTT AACCGTAGCC ATTACCGATC CTAATGCTGG AATCCGCCGG 
ACAGTGCTCG ATCACCTCGA AGACAAGTTT GATCGCCATC TCGCTCAGGC TGACGATATC 
CGATGTCTCT TTATCGCTCT CAACGATGAA GTTTTTGGTA ATCGTGAGAG GACTATCTCG 
ATCATTGGGA GATTGGCACA CCATAACCCG GCTTATGTGA TGCCTCATTT GAGAAAGAGT 
TTGATCAATA TTGTCACTGA GTTGGAGTAT TCTACCAATG CGCGACAAAA GGAAGAGAGT 
GCCAAGCTGC TCTGTCTTAT AATCGGCGCT GCCGCAGGTC TCGTCAAGTC TTATGCCCCT 
ACCATCCTAT CCGTTCTTCT CCGCACTGCT TCCAGCCCCG ATTCTTCTAT CGGTGTCCAA 
GCCGAATGCC TCAAATGTAT TGGTGAGCTT GCCAGAGTTG CGGGCGAAGA ACTCGTCCCC 
TCCGTCCGAG CCATTCTCGA CCTCGTCATT GAAATGCTCA ATGACCAGGC TTCCCCCGCC 
AAGAGAGATA CTGCCCTCAA GACTTTAGGG CAGATTGCGA GTAACACAGG AGAAGTTATC 
AAACCCTATA CGGATTACCC TCAGTTGATG GGGGTGCTGT TTAGGTTTTT GAGAATGGAG 
GCGAATTCGA GTGTCAGGCA AGAGACGATC AAGACGATTG GTATGCTCGG TGCCTTGGAT 
CCCTTTAAGC ACAAGACTTT GTTGGGTGAT GTGGACGACC CTATCGACGA AGGGACAACC 
TCGCGAGTCA ATGACATTGT GTTGCTCAAC CAACACAACT CTTCAGTCAA TGACGAGTTC 
TTCCAGACTG TTGTCATCCA TTCTTTAGTC AACGTCTTGC ATGACTCTAC TTACAAGGAT 
CATTATCAAG CCGTGGAAGC AATTATGATG ATTTTTAGGA CTCAACGATT GAGATGTGTC 
AACTTCCTCC CTCAGATCGT CCCTGCATTC CTCAATGTCA TTCGCATTGC ACATTCCTCT 
CGTACCGAGC TTTACCTCAA ACAACTTGCG CAATTTATTA CAATCGTTAA ACTGCATATC 
CGCAACTACC TCAATGATGT CTTTGATCTA ATCCACGAGT TCTGGAACCC CAACTCTACT 
CTTCAAATTA CCATCATCTC CCTCGTTGAG GCCATCGCGA AGGCTGTGGA AGGTGAATTC 
AAGGCATACT TGCCCAAGCT TTTGCAGCAG ATTTTGAGAT CGTTTGATGG AGACTTGTCA 
GCCAAGCACC TCCCGGAGCT GAAACTCAAC ACTTTGCTTC AGATTCTGAA AGCCTTCTAC 
GTCTTTGGCG AATCTATCGA AGACTACCTC CACCTTGTCT TGCCCGTCAT TGTTCGATCT 
TTTGAGAACC CGGCCGCCCC CGATTCTCTC CGCATTGCTG CTTTGCGCAC TACAGGACAG 
CTTTGTCGTA AAGTCAACTT TTCGGACCAT GCGAGTCAGA TCATCCACCC GTTGGTAAGA 
ACTTTGGGTA ATAGCTCAGA GGAGTTGAGG CAGACTGCAA TGGAAACACT TTGCGTTTTG 
GTGTTGCAGT TCGGCCCAGA TTATGCTATC TTCATTCCTA TGGTAAACAA AGCTTTGGTG 
GAAAACAAAA TATCACATCC TGGTTACGAA GCGCTCATAA CCCAATTGCT CAACCGCGAG 
CGCCTTCCTC CAGATCTTGG CCCTGTCGAA CGATACGCCA GTGACTCTGC CGTCGAAGCC 
TCTGCCCCAG AGCCTGTTGC TCTTAAGGTC AACCAGCAGG CTCTTAAACT GGCCTGGGAC 
TGTTCCCACT TGCTTAACAC CAGCAGCAGG ACCGAGTGGA TCTCTTGGAT TATTGGCTTG 
GGTCATGAGA TGATGAGAGA AAGCCCTAGT CAAGCCATAA GGGCCGCAAG GAGTTTGGCG 
TTGAGTAGTG TGGCCTTCAC CAAGGAACTT TTCAATGTTG CTTTCTACTC ATGCTGGCAG 
GAGCTGTTTG AGAGTTACCA GGAGGATTTA TGGCATAATC TTGACCGAGC AATCAAGAAA 
GATGATGTCC CCGGCGATGT TGTCAATATG ATTCTCGGGG CCACTCAGTT CTTGGAGCAT 
GATGAAAAGG AAGTAGCTAT CGAAAGTCGT GTTTTGGGCA GTGTCGCTGC CAATTATCAA 
GCTCTCGCGG TCGCTTTACA TTACAAAGAA CAGGAATTCT TCCTGGACCC TAGCAAGGAA 
GTCATCGAAG ACCTCATTGA CGTCAACCAG AAACTCCAGC AAAGCGATGC CGCCTGGGGT 
ACCCTTGAAT GGGCTCAGAC AGAGATGGGT ATGACTACTG AGGTCGAGTG GTACGAAAAG 
CTTGGAAGGT GGGAGGAAGC GTTACAAGTG TGGAACGAGC GAGATGCCGA TGCTTCGACC 
ACTTTCTCAG AGTGGGAGAT CACTGAAGGC AAGGTCACTT GTCTGCACGC TATGGGCGAA 
TGGGAACAAC TCTCCGACTT TGTCCAGGCT CGATGGGCCA ACAGGACGGC AGAGGAGAAA 
AAGTTGCTCT CGCCGTTAGC GGCAGCAGCT AGTTGGTCGC TGAAACAATG GGATCTGATG 
GACGATTACA TCTCCGCCAT GAAGGGCGAC GGAGCGGATC GCGCGTTCTT CAAGGCTATC 
TTAGCTGTGC ACAGAAACCA AATCCCTGCT GCTTTGAAGC AAATCTCCAA GGCGCGAGAA 
AGGCTGGATC CAGAGTTGAC TACCTTAACT GGTGACAGCT ACGGCCGAGC GTACGATACT 
GTCGTGAGGA TTCAAATGCT TGCTGAGCTT GAGGAAATCA TTGCCTACAA GGATCACGCC 
GACGAGCCTG CCCGACAGGA GATGCAACGA CAGACTTGGA AGAAGCGTTT GGCTGGATGT 
CAGCGCGATG TCGAAGTTTG GCAGCGCATT CTTCAGGTCA GATCCCTTGT ACTCAAGCCT 
AATGAGGATA TGGACACTTG GATTGAGTTT GCGGACCTCT GTCGAACATC TGACAGGCTG 
AACTTGGCCG AGAAAACGCT GACATCTCTT GTTGGCTTCC AATACCCATC AATGGAAGAC 
ACTCGAGGAC GAGCACCACC TCCCATCATT TTCGCTTACC TACGTATGGC CTGGGCGAAG 
AACCTTCAAA TCGACTCTCG AGAAGAACGT TACGAAACTC TCCAACACTT GCGAGACTTC 
ACGGACCAGC TTACCGACGA TGTTGGTATC GGAGCGAGGG GCCCCAACGG CAGACTCATG 
TTACCTGACC AGAAGTTGTA TGGATCTTAC ACCAAGCTGC TGGCGCAGTG CCATGTTGAA 
TTGGGTCAGT GGCAAGCGAC TTTGAGGGAA AGCCAAGGAT CTGCTGATCC CTCGGGCATT 
CTCCACGACT ATTCTCTCGC CACTGAACTT GACCCTGAGT GGTACCAAGC TTGGCACACT 
TGGGCTTTGG CCAATTTCGA AGTTATTACC CAGCTTGAAG TATCACAGCA AGGTCTCTCA 
CCTATTCACT TTACGACCTA TATCATCCCT GCTGTCGAAG GTTTCCTCAA GTCTATCTCG 
CTTTCTCCTG GCAACTCCTT GCAAGATACT TTAAGGCTGC TGACTCTCTG GTTTACATAT 
GGATACTCTA GCGGAGTGAC AGCGGCCGTT AGCCAAGGTC TGCCCACTGT CAACATTGAT 
GTCTGGCTCG AGGTCATCCC TCAGATTATT GCCCGTATCC AAACGCCACG CCAGTCTATA 
CAGCAGCTCA TTGTACAGCT CTTGCATGAT ATTGGCAAAG CCCATCCTCA AGCCCTTATC 
TACCCTCTTA CCGTCGCCTC CAAATCTACA GTCGCAGCCC GACGCACTGT TGCTCAAAAT 
ATCACCCATA AAATGCGAGA GCACTCTCCC AAGATCGTTG ACCAGGCCGA GCTTGTCAGT 
ACTGAGCTCA TCCGAGCGGC TATCTTATGG CATGAGATGT GGTATGATGG TTTGGAAGAA 
GCGTCAAAGC ACTACTTTGG TGACCATGAT ATCCCTGGCA TGCTGGGAGT TCTTGAACCT 
TTGCATGAGA TTGTCGAAAA CGGACCCCAA ACCTTGCGTG AGACGTCCTT TATTCAATCG 
TTCGGGCATG ATTTGCGTAT CGCCCGAGAG CATCTCAAGC GTTACCGTAT AACTCAGGAT 
GGGACCGAAA TTCAACAAGC ATGGGATGTC TACTACTCCG TCTTCCAGCG TCTCGGCAAA 
CAGCTCAAGC TCCTGAACGT CATTGAGCTG CAATATGTCT CGCCCAAGTT GATGGCCGTT 
CGAGACTTGG ATATTGCTGT TCCAGGTACC TACCAGAGTG GCAAGCCTAT CATCGGTATT 
AAGAACGTTA TCCCGACCTT CAAGGTCATT GCTTCCAAGC AAAAGCCAAG ACAATGCAGC 
ATGCGCGGTA TGGATGGTAA AGAATATGCG TACTGCCTCA AGGGTCACGA GGACTTGCGA 
CAAGACGAGC GTGTTATGCA GCTCTTCGGT TTGGTCAACA CTCTTCTTAA TAATGATCAC 
GAGTCTGCCA AACGACATCT CAGTATCCAG CGATTTTCTG TTACTCCTCT TTCCCCTAGT 
GCCGGTTTAC TCGGTTGGGT TACCCACAGT GATACCATCC ACGTCCTCAT CAAACAATAC 
CGAGACCAAA GGAAAATCTT GGTGGATATT GAGCACAAAC TTATGCAGCA GATGTCTGAT 
GAGAGCTACG ATTCTCTGCC GCTCTTGCAC AAGGTCGAGA TCTTCCAGTA TGCCTTGGAT 
AACACAACTG GTCAGGATTT GTACCGCATC TTGTGGCTCA AGTCTCGTAA CTCAGATATC 
TGGCTTGAGA GAAGGACCAC CTACACTAGA AGTCTGGGTC TCAACTCGAT GGTCGGGTAT 
ATCCTTGGCC TGGGTGACAG ACATCCTTCA AACCTGTTGC TCGATCAGAT TACGGGTAAG 
ATGGTGCACA TTGACTTTGG TGATTGTTTC GAGGTTGCTC AACAGAGAGA CAAGTACCCT 
GAGAAAGTAC CGTTCCGACT TACCCGAATG CTCATTCATG CCATGGAGGT CTGCGGTATC 
ACTGGCAACT TTTCGCGAAG CTGTGAAGTG TCCATGGAAG TCCTTCGTGA CAACAGAGAA 
TCACTTATGG CCGTGCTTGA AGCATTTGTG TACGATCCTC TTATTGCTTG GCGTCTTACG 
GCGACGGACA AACGACCTGG TGGCGTTGGC GAAGTCAAGG ACCTGGATGA CCCGGCGGTG 
TACGGAAAGC AGAGGAAAAA CAAGGCGAAC GAGACAGAGA TTCTCAATGA TGTAGAAAAT 
ACCGAGGTGA AGAATGACAA GGGTCTGCAG GTCATTGAAC GAGTACGACG AAAATTGACC 
GGTCGAGATT TTAAGCCCGA CGTTGTACTC GATGTCAAGT CCCAAGTGGA GAAATTGGTG 
GTTGAGGCGA CAAAGACAGA GAACCTGTGT GTGGCGTTCT TGGGATGGTG TTCCTTCTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_571336.1
CDS	139..7221
Translation	MSSQSDVLDNIFQRLSARSEDVRAQAGQDLAEHVVAYTQEYPGHDASKGVWAQVFHKTFEFTRSNNQLERLGAIISISQLLQLTKDDTLDRAQQKVLRLYEYLRPLTTCGDSTVMLPASLVVEDMVRNSPTLHTDTFLGKEVGQALVMIDDSRQEVGRFSGALLLYAFARAAPGVFHQYIPKVLEKIWVPLRDSRSVVRERASMLLSTCLDTLKTRGDRPSTDTYRKIFEEARLGLLKASSTESILGSLLAFNSMLQNQQLSMAEYYRSICELTFKYRDSKEVSIRKAVIALIPSMATYDSDDFEAHYLHRSMAYLLQALNRPADRDISYVALGHMAVHLGSKMKPFIDDIMRIIRDHLRMRGKKNAPYEAPIFQCLAMLATSVGPMLTRQMHEILNLMFPWGLSEPLCTALQATASHIPPLLRTIQDRLLEMLSQTLTGHSYRPLGAPAPRGGAQMDLNLLQSTTNAQSADTLKLALRLLARFDFVGHTLSEFVRDAALPYLEHDSVEVRREAVLATTTLFMTDPICQQTSSNSVEIVNDVLSKLLTVAITDPNAGIRRTVLDHLEDKFDRHLAQADDIRCLFIALNDEVFGNRERTISIIGRLAHHNPAYVMPHLRKSLINIVTELEYSTNARQKEESAKLLCLIIGAAAGLVKSYAPTILSVLLRTASSPDSSIGVQAECLKCIGELARVAGEELVPSVRAILDLVIEMLNDQASPAKRDTALKTLGQIASNTGEVIKPYTDYPQLMGVLFRFLRMEANSSVRQETIKTIGMLGALDPFKHKTLLGDVDDPIDEGTTSRVNDIVLLNQHNSSVNDEFFQTVVIHSLVNVLHDSTYKDHYQAVEAIMMIFRTQRLRCVNFLPQIVPAFLNVIRIAHSSRTELYLKQLAQFITIVKLHIRNYLNDVFDLIHEFWNPNSTLQITIISLVEAIAKAVEGEFKAYLPKLLQQILRSFDGDLSAKHLPELKLNTLLQILKAFYVFGESIEDYLHLVLPVIVRSFENPAAPDSLRIAALRTTGQLCRKVNFSDHASQIIHPLVRTLGNSSEELRQTAMETLCVLVLQFGPDYAIFIPMVNKALVENKISHPGYEALITQLLNRERLPPDLGPVERYASDSAVEASAPEPVALKVNQQALKLAWDCSHLLNTSSRTEWISWIIGLGHEMMRESPSQAIRAARSLALSSVAFTKELFNVAFYSCWQELFESYQEDLWHNLDRAIKKDDVPGDVVNMILGATQFLEHDEKEVAIESRVLGSVAANYQALAVALHYKEQEFFLDPSKEVIEDLIDVNQKLQQSDAAWGTLEWAQTEMGMTTEVEWYEKLGRWEEALQVWNERDADASTTFSEWEITEGKVTCLHAMGEWEQLSDFVQARWANRTAEEKKLLSPLAAAASWSLKQWDLMDDYISAMKGDGADRAFFKAILAVHRNQIPAALKQISKARERLDPELTTLTGDSYGRAYDTVVRIQMLAELEEIIAYKDHADEPARQEMQRQTWKKRLAGCQRDVEVWQRILQVRSLVLKPNEDMDTWIEFADLCRTSDRLNLAEKTLTSLVGFQYPSMEDTRGRAPPPIIFAYLRMAWAKNLQIDSREERYETLQHLRDFTDQLTDDVGIGARGPNGRLMLPDQKLYGSYTKLLAQCHVELGQWQATLRESQGSADPSGILHDYSLATELDPEWYQAWHTWALANFEVITQLEVSQQGLSPIHFTTYIIPAVEGFLKSISLSPGNSLQDTLRLLTLWFTYGYSSGVTAAVSQGLPTVNIDVWLEVIPQIIARIQTPRQSIQQLIVQLLHDIGKAHPQALIYPLTVASKSTVAARRTVAQNITHKMREHSPKIVDQAELVSTELIRAAILWHEMWYDGLEEASKHYFGDHDIPGMLGVLEPLHEIVENGPQTLRETSFIQSFGHDLRIAREHLKRYRITQDGTEIQQAWDVYYSVFQRLGKQLKLLNVIELQYVSPKLMAVRDLDIAVPGTYQSGKPIIGIKNVIPTFKVIASKQKPRQCSMRGMDGKEYAYCLKGHEDLRQDERVMQLFGLVNTLLNNDHESAKRHLSIQRFSVTPLSPSAGLLGWVTHSDTIHVLIKQYRDQRKILVDIEHKLMQQMSDESYDSLPLLHKVEIFQYALDNTTGQDLYRILWLKSRNSDIWLERRTTYTRSLGLNSMVGYILGLGDRHPSNLLLDQITGKMVHIDFGDCFEVAQQRDKYPEKVPFRLTRMLIHAMEVCGITGNFSRSCEVSMEVLRDNRESLMAVLEAFVYDPLIAWRLTATDKRPGGVGEVKDLDDPAVYGKQRKNKANETEILNDVENTEVKNDKGLQVIERVRRKLTGRDFKPDVVLDVKSQVEKLVVEATKTENLCVAFLGWCSFW

Target ORF information:

RefSeq Version	XM_571336.2
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1 (CNF03740), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_571336.2

ORF Insert Sequence:

ATGTCTTCAC AATCAGACGT TCTCGACAAC ATCTTTCAGC GTCTGTCTGC CAGGTCGGAG 
GATGTCCGCG CTCAGGCCGG TCAAGATCTT GCAGAGCACG TCGTAGCTTA TACCCAGGAA 
TACCCGGGAC ATGATGCTTC GAAGGGTGTA TGGGCACAGG TCTTTCACAA GACATTCGAG 
TTTACTAGGA GCAACAATCA GCTAGAAAGA TTAGGTGCAA TTATTTCCAT CTCGCAATTG 
TTACAACTAA CGAAGGATGA CACACTGGAT CGTGCTCAAC AAAAGGTTCT ACGCCTCTAT 
GAATATCTTC GGCCTCTCAC AACATGCGGC GATTCGACCG TCATGCTTCC CGCCTCGCTC 
GTCGTTGAAG ATATGGTCCG CAATTCCCCC ACCCTTCACA CCGATACTTT CCTTGGCAAA 
GAAGTTGGAC AAGCGCTGGT AATGATTGAC GACTCACGTC AAGAAGTCGG TCGTTTCAGT 
GGTGCTCTTC TTCTCTATGC TTTCGCCCGT GCTGCCCCCG GAGTATTCCA TCAATACATC 
CCCAAGGTCT TGGAAAAGAT TTGGGTTCCC CTTCGCGACT CAAGATCTGT CGTTCGAGAA 
CGTGCGAGCA TGCTCTTATC TACCTGCCTT GACACTCTCA AAACCCGAGG AGACCGACCA 
TCAACCGACA CCTACCGCAA GATCTTTGAA GAGGCCCGTC TTGGTCTCCT CAAAGCCAGC 
TCAACAGAGT CTATCTTGGG TTCTCTCCTT GCTTTCAATT CCATGCTTCA AAATCAACAG 
CTTTCGATGG CGGAATATTA TCGCTCCATT TGCGAACTAA CCTTCAAATA CCGTGATTCG 
AAGGAAGTTT CCATCAGGAA GGCTGTCATC GCCCTCATAC CTTCTATGGC GACATATGAT 
AGCGACGACT TTGAGGCACA TTATTTGCAT AGGAGTATGG CGTATTTGCT ACAAGCGTTG 
AACAGGCCGG CGGACAGGGA CATTTCGTAT GTGGCATTGG GTCATATGGC CGTACATCTT 
GGATCGAAAA TGAAACCTTT TATTGACGAC ATTATGCGGA TCATTCGAGA TCATCTACGC 
ATGCGAGGCA AAAAAAATGC TCCATACGAA GCCCCCATCT TCCAGTGTCT TGCGATGCTC 
GCTACCTCTG TCGGCCCTAT GCTTACCCGC CAGATGCACG AAATCCTCAA CCTGATGTTC 
CCCTGGGGCC TTTCGGAACC CTTATGTACA GCCCTTCAAG CCACTGCCTC ACATATCCCT 
CCTCTATTGA GGACTATCCA AGATAGGTTG TTGGAGATGC TTTCACAGAC TTTGACCGGA 
CACAGCTATA GACCGTTAGG TGCGCCTGCC CCAAGAGGTG GAGCGCAGAT GGATCTTAAC 
CTTTTGCAAT CTACAACCAA TGCCCAGTCA GCAGACACCC TAAAACTTGC TCTTCGTCTC 
CTTGCGCGCT TTGATTTTGT CGGCCATACC CTTAGCGAGT TTGTCCGTGA CGCTGCTCTT 
CCGTATCTGG AACACGACAG TGTCGAGGTC CGACGTGAAG CCGTGTTGGC GACCACGACC 
CTGTTCATGA CGGACCCGAT CTGTCAACAG ACGAGCAGTA ATTCGGTCGA GATTGTAAAT 
GATGTGTTGA GTAAATTGTT AACCGTAGCC ATTACCGATC CTAATGCTGG AATCCGCCGG 
ACAGTGCTCG ATCACCTCGA AGACAAGTTT GATCGCCATC TCGCTCAGGC TGACGATATC 
CGATGTCTCT TTATCGCTCT CAACGATGAA GTTTTTGGTA ATCGTGAGAG GACTATCTCG 
ATCATTGGGA GATTGGCACA CCATAACCCG GCTTATGTGA TGCCTCATTT GAGAAAGAGT 
TTGATCAATA TTGTCACTGA GTTGGAGTAT TCTACCAATG CGCGACAAAA GGAAGAGAGT 
GCCAAGCTGC TCTGTCTTAT AATCGGCGCT GCCGCAGGTC TCGTCAAGTC TTATGCCCCT 
ACCATCCTAT CCGTTCTTCT CCGCACTGCT TCCAGCCCCG ATTCTTCTAT CGGTGTCCAA 
GCCGAATGCC TCAAATGTAT TGGTGAGCTT GCCAGAGTTG CGGGCGAAGA ACTCGTCCCC 
TCCGTCCGAG CCATTCTCGA CCTCGTCATT GAAATGCTCA ATGACCAGGC TTCCCCCGCC 
AAGAGAGATA CTGCCCTCAA GACTTTAGGG CAGATTGCGA GTAACACAGG AGAAGTTATC 
AAACCCTATA CGGATTACCC TCAGTTGATG GGGGTGCTGT TTAGGTTTTT GAGAATGGAG 
GCGAATTCGA GTGTCAGGCA AGAGACGATC AAGACGATTG GTATGCTCGG TGCCTTGGAT 
CCCTTTAAGC ACAAGACTTT GTTGGGTGAT GTGGACGACC CTATCGACGA AGGGACAACC 
TCGCGAGTCA ATGACATTGT GTTGCTCAAC CAACACAACT CTTCAGTCAA TGACGAGTTC 
TTCCAGACTG TTGTCATCCA TTCTTTAGTC AACGTCTTGC ATGACTCTAC TTACAAGGAT 
CATTATCAAG CCGTGGAAGC AATTATGATG ATTTTTAGGA CTCAACGATT GAGATGTGTC 
AACTTCCTCC CTCAGATCGT CCCTGCATTC CTCAATGTCA TTCGCATTGC ACATTCCTCT 
CGTACCGAGC TTTACCTCAA ACAACTTGCG CAATTTATTA CAATCGTTAA ACTGCATATC 
CGCAACTACC TCAATGATGT CTTTGATCTA ATCCACGAGT TCTGGAACCC CAACTCTACT 
CTTCAAATTA CCATCATCTC CCTCGTTGAG GCCATCGCGA AGGCTGTGGA AGGTGAATTC 
AAGGCATACT TGCCCAAGCT TTTGCAGCAG ATTTTGAGAT CGTTTGATGG AGACTTGTCA 
GCCAAGCACC TCCCGGAGCT GAAACTCAAC ACTTTGCTTC AGATTCTGAA AGCCTTCTAC 
GTCTTTGGCG AATCTATCGA AGACTACCTC CACCTTGTCT TGCCCGTCAT TGTTCGATCT 
TTTGAGAACC CGGCCGCCCC CGATTCTCTC CGCATTGCTG CTTTGCGCAC TACAGGACAG 
CTTTGTCGTA AAGTCAACTT TTCGGACCAT GCGAGTCAGA TCATCCACCC GTTGGTAAGA 
ACTTTGGGTA ATAGCTCAGA GGAGTTGAGG CAGACTGCAA TGGAAACACT TTGCGTTTTG 
GTGTTGCAGT TCGGCCCAGA TTATGCTATC TTCATTCCTA TGGTAAACAA AGCTTTGGTG 
GAAAACAAAA TATCACATCC TGGTTACGAA GCGCTCATAA CCCAATTGCT CAACCGCGAG 
CGCCTTCCTC CAGATCTTGG CCCTGTCGAA CGATACGCCA GTGACTCTGC CGTCGAAGCC 
TCTGCCCCAG AGCCTGTTGC TCTTAAGGTC AACCAGCAGG CTCTTAAACT GGCCTGGGAC 
TGTTCCCACT TGCTTAACAC CAGCAGCAGG ACCGAGTGGA TCTCTTGGAT TATTGGCTTG 
GGTCATGAGA TGATGAGAGA AAGCCCTAGT CAAGCCATAA GGGCCGCAAG GAGTTTGGCG 
TTGAGTAGTG TGGCCTTCAC CAAGGAACTT TTCAATGTTG CTTTCTACTC ATGCTGGCAG 
GAGCTGTTTG AGAGTTACCA GGAGGATTTA TGGCATAATC TTGACCGAGC AATCAAGAAA 
GATGATGTCC CCGGCGATGT TGTCAATATG ATTCTCGGGG CCACTCAGTT CTTGGAGCAT 
GATGAAAAGG AAGTAGCTAT CGAAAGTCGT GTTTTGGGCA GTGTCGCTGC CAATTATCAA 
GCTCTCGCGG TCGCTTTACA TTACAAAGAA CAGGAATTCT TCCTGGACCC TAGCAAGGAA 
GTCATCGAAG ACCTCATTGA CGTCAACCAG AAACTCCAGC AAAGCGATGC CGCCTGGGGT 
ACCCTTGAAT GGGCTCAGAC AGAGATGGGT ATGACTACTG AGGTCGAGTG GTACGAAAAG 
CTTGGAAGGT GGGAGGAAGC GTTACAAGTG TGGAACGAGC GAGATGCCGA TGCTTCGACC 
ACTTTCTCAG AGTGGGAGAT CACTGAAGGC AAGGTCACTT GTCTGCACGC TATGGGCGAA 
TGGGAACAAC TCTCCGACTT TGTCCAGGCT CGATGGGCCA ACAGGACGGC AGAGGAGAAA 
AAGTTGCTCT CGCCGTTAGC GGCAGCAGCT AGTTGGTCGC TGAAACAATG GGATCTGATG 
GACGATTACA TCTCCGCCAT GAAGGGCGAC GGAGCGGATC GCGCGTTCTT CAAGGCTATC 
TTAGCTGTGC ACAGAAACCA AATCCCTGCT GCTTTGAAGC AAATCTCCAA GGCGCGAGAA 
AGGCTGGATC CAGAGTTGAC TACCTTAACT GGTGACAGCT ACGGCCGAGC GTACGATACT 
GTCGTGAGGA TTCAAATGCT TGCTGAGCTT GAGGAAATCA TTGCCTACAA GGATCACGCC 
GACGAGCCTG CCCGACAGGA GATGCAACGA CAGACTTGGA AGAAGCGTTT GGCTGGATGT 
CAGCGCGATG TCGAAGTTTG GCAGCGCATT CTTCAGGTCA GATCCCTTGT ACTCAAGCCT 
AATGAGGATA TGGACACTTG GATTGAGTTT GCGGACCTCT GTCGAACATC TGACAGGCTG 
AACTTGGCCG AGAAAACGCT GACATCTCTT GTTGGCTTCC AATACCCATC AATGGAAGAC 
ACTCGAGGAC GAGCACCACC TCCCATCATT TTCGCTTACC TACGTATGGC CTGGGCGAAG 
AACCTTCAAA TCGACTCTCG AGAAGAACGT TACGAAACTC TCCAACACTT GCGAGACTTC 
ACGGACCAGC TTACCGACGA TGTTGGTATC GGAGCGAGGG GCCCCAACGG CAGACTCATG 
TTACCTGACC AGAAGTTGTA TGGATCTTAC ACCAAGCTGC TGGCGCAGTG CCATGTTGAA 
TTGGGTCAGT GGCAAGCGAC TTTGAGGGAA AGCCAAGGAT CTGCTGATCC CTCGGGCATT 
CTCCACGACT ATTCTCTCGC CACTGAACTT GACCCTGAGT GGTACCAAGC TTGGCACACT 
TGGGCTTTGG CCAATTTCGA AGTTATTACC CAGCTTGAAG TATCACAGCA AGGTCTCTCA 
CCTATTCACT TTACGACCTA TATCATCCCT GCTGTCGAAG GTTTCCTCAA GTCTATCTCG 
CTTTCTCCTG GCAACTCCTT GCAAGATACT TTAAGGCTGC TGACTCTCTG GTTTACATAT 
GGATACTCTA GCGGAGTGAC AGCGGCCGTT AGCCAAGGTC TGCCCACTGT CAACATTGAT 
GTCTGGCTCG AGGTCATCCC TCAGATTATT GCCCGTATCC AAACGCCACG CCAGTCTATA 
CAGCAGCTCA TTGTACAGCT CTTGCATGAT ATTGGCAAAG CCCATCCTCA AGCCCTTATC 
TACCCTCTTA CCGTCGCCTC CAAATCTACA GTCGCAGCCC GACGCACTGT TGCTCAAAAT 
ATCACCCATA AAATGCGAGA GCACTCTCCC AAGATCGTTG ACCAGGCCGA GCTTGTCAGT 
ACTGAGCTCA TCCGAGCGGC TATCTTATGG CATGAGATGT GGTATGATGG TTTGGAAGAA 
GCGTCAAAGC ACTACTTTGG TGACCATGAT ATCCCTGGCA TGCTGGGAGT TCTTGAACCT 
TTGCATGAGA TTGTCGAAAA CGGACCCCAA ACCTTGCGTG AGACGTCCTT TATTCAATCG 
TTCGGGCATG ATTTGCGTAT CGCCCGAGAG CATCTCAAGC GTTACCGTAT AACTCAGGAT 
GGGACCGAAA TTCAACAAGC ATGGGATGTC TACTACTCCG TCTTCCAGCG TCTCGGCAAA 
CAGCTCAAGC TCCTGAACGT CATTGAGCTG CAATATGTCT CGCCCAAGTT GATGGCCGTT 
CGAGACTTGG ATATTGCTGT TCCAGGTACC TACCAGAGTG GCAAGCCTAT CATCGGTATT 
AAGAACGTTA TCCCGACCTT CAAGGTCATT GCTTCCAAGC AAAAGCCAAG ACAATGCAGC 
ATGCGCGGTA TGGATGGTAA AGAATATGCG TACTGCCTCA AGGGTCACGA GGACTTGCGA 
CAAGACGAGC GTGTTATGCA GCTCTTCGGT TTGGTCAACA CTCTTCTTAA TAATGATCAC 
GAGTCTGCCA AACGACATCT CAGTATCCAG CGATTTTCTG TTACTCCTCT TTCCCCTAGT 
GCCGGTTTAC TCGGTTGGGT TACCCACAGT GATACCATCC ACGTCCTCAT CAAACAATAC 
CGAGACCAAA GGAAAATCTT GGTGGATATT GAGCACAAAC TTATGCAGCA GATGTCTGAT 
GAGAGCTACG ATTCTCTGCC GCTCTTGCAC AAGGTCGAGA TCTTCCAGTA TGCCTTGGAT 
AACACAACTG GTCAGGATTT GTACCGCATC TTGTGGCTCA AGTCTCGTAA CTCAGATATC 
TGGCTTGAGA GAAGGACCAC CTACACTAGA AGTCTGGGTC TCAACTCGAT GGTCGGGTAT 
ATCCTTGGCC TGGGTGACAG ACATCCTTCA AACCTGTTGC TCGATCAGAT TACGGGTAAG 
ATGGTGCACA TTGACTTTGG TGATTGTTTC GAGGTTGCTC AACAGAGAGA CAAGTACCCT 
GAGAAAGTAC CGTTCCGACT TACCCGAATG CTCATTCATG CCATGGAGGT CTGCGGTATC 
ACTGGCAACT TTTCGCGAAG CTGTGAAGTG TCCATGGAAG TCCTTCGTGA CAACAGAGAA 
TCACTTATGG CCGTGCTTGA AGCATTTGTG TACGATCCTC TTATTGCTTG GCGTCTTACG 
GCGACGGACA AACGACCTGG TGGCGTTGGC GAAGTCAAGG ACCTGGATGA CCCGGCGGTG 
TACGGAAAGC AGAGGAAAAA CAAGGCGAAC GAGACAGAGA TTCTCAATGA TGTAGAAAAT 
ACCGAGGTGA AGAATGACAA GGGTCTGCAG GTCATTGAAC GAGTACGACG AAAATTGACC 
GGTCGAGATT TTAAGCCCGA CGTTGTACTC GATGTCAAGT CCCAAGTGGA GAAATTGGTG 
GTTGAGGCGA CAAAGACAGA GAACCTGTGT GTGGCGTTCT TGGGATGGTG TTCCTTCTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNF03740 ( XM_571336.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_571336.1 Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1, partial mRNA.

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CloneID	OCn04903
Clone ID Related Accession (Same CDS sequence)	XM_571336.2 , XM_571336.1
Accession Version	XM_571336.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1345046400000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	phosphatidylinositol 3-kinase TOR1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006691). The reference sequence was derived from mrna.CNF03740.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/). COMPLETENESS: incomplete on the 5' end.

ATGTCTTCAC AATCAGACGT TCTCGACAAC ATCTTTCAGC GTCTGTCTGC CAGGTCGGAG 
GATGTCCGCG CTCAGGCCGG TCAAGATCTT GCAGAGCACG TCGTAGCTTA TACCCAGGAA 
TACCCGGGAC ATGATGCTTC GAAGGGTGTA TGGGCACAGG TCTTTCACAA GACATTCGAG 
TTTACTAGGA GCAACAATCA GCTAGAAAGA TTAGGTGCAA TTATTTCCAT CTCGCAATTG 
TTACAACTAA CGAAGGATGA CACACTGGAT CGTGCTCAAC AAAAGGTTCT ACGCCTCTAT 
GAATATCTTC GGCCTCTCAC AACATGCGGC GATTCGACCG TCATGCTTCC CGCCTCGCTC 
GTCGTTGAAG ATATGGTCCG CAATTCCCCC ACCCTTCACA CCGATACTTT CCTTGGCAAA 
GAAGTTGGAC AAGCGCTGGT AATGATTGAC GACTCACGTC AAGAAGTCGG TCGTTTCAGT 
GGTGCTCTTC TTCTCTATGC TTTCGCCCGT GCTGCCCCCG GAGTATTCCA TCAATACATC 
CCCAAGGTCT TGGAAAAGAT TTGGGTTCCC CTTCGCGACT CAAGATCTGT CGTTCGAGAA 
CGTGCGAGCA TGCTCTTATC TACCTGCCTT GACACTCTCA AAACCCGAGG AGACCGACCA 
TCAACCGACA CCTACCGCAA GATCTTTGAA GAGGCCCGTC TTGGTCTCCT CAAAGCCAGC 
TCAACAGAGT CTATCTTGGG TTCTCTCCTT GCTTTCAATT CCATGCTTCA AAATCAACAG 
CTTTCGATGG CGGAATATTA TCGCTCCATT TGCGAACTAA CCTTCAAATA CCGTGATTCG 
AAGGAAGTTT CCATCAGGAA GGCTGTCATC GCCCTCATAC CTTCTATGGC GACATATGAT 
AGCGACGACT TTGAGGCACA TTATTTGCAT AGGAGTATGG CGTATTTGCT ACAAGCGTTG 
AACAGGCCGG CGGACAGGGA CATTTCGTAT GTGGCATTGG GTCATATGGC CGTACATCTT 
GGATCGAAAA TGAAACCTTT TATTGACGAC ATTATGCGGA TCATTCGAGA TCATCTACGC 
ATGCGAGGCA AAAAAAATGC TCCATACGAA GCCCCCATCT TCCAGTGTCT TGCGATGCTC 
GCTACCTCTG TCGGCCCTAT GCTTACCCGC CAGATGCACG AAATCCTCAA CCTGATGTTC 
CCCTGGGGCC TTTCGGAACC CTTATGTACA GCCCTTCAAG CCACTGCCTC ACATATCCCT 
CCTCTATTGA GGACTATCCA AGATAGGTTG TTGGAGATGC TTTCACAGAC TTTGACCGGA 
CACAGCTATA GACCGTTAGG TGCGCCTGCC CCAAGAGGTG GAGCGCAGAT GGATCTTAAC 
CTTTTGCAAT CTACAACCAA TGCCCAGTCA GCAGACACCC TAAAACTTGC TCTTCGTCTC 
CTTGCGCGCT TTGATTTTGT CGGCCATACC CTTAGCGAGT TTGTCCGTGA CGCTGCTCTT 
CCGTATCTGG AACACGACAG TGTCGAGGTC CGACGTGAAG CCGTGTTGGC GACCACGACC 
CTGTTCATGA CGGACCCGAT CTGTCAACAG ACGAGCAGTA ATTCGGTCGA GATTGTAAAT 
GATGTGTTGA GTAAATTGTT AACCGTAGCC ATTACCGATC CTAATGCTGG AATCCGCCGG 
ACAGTGCTCG ATCACCTCGA AGACAAGTTT GATCGCCATC TCGCTCAGGC TGACGATATC 
CGATGTCTCT TTATCGCTCT CAACGATGAA GTTTTTGGTA ATCGTGAGAG GACTATCTCG 
ATCATTGGGA GATTGGCACA CCATAACCCG GCTTATGTGA TGCCTCATTT GAGAAAGAGT 
TTGATCAATA TTGTCACTGA GTTGGAGTAT TCTACCAATG CGCGACAAAA GGAAGAGAGT 
GCCAAGCTGC TCTGTCTTAT AATCGGCGCT GCCGCAGGTC TCGTCAAGTC TTATGCCCCT 
ACCATCCTAT CCGTTCTTCT CCGCACTGCT TCCAGCCCCG ATTCTTCTAT CGGTGTCCAA 
GCCGAATGCC TCAAATGTAT TGGTGAGCTT GCCAGAGTTG CGGGCGAAGA ACTCGTCCCC 
TCCGTCCGAG CCATTCTCGA CCTCGTCATT GAAATGCTCA ATGACCAGGC TTCCCCCGCC 
AAGAGAGATA CTGCCCTCAA GACTTTAGGG CAGATTGCGA GTAACACAGG AGAAGTTATC 
AAACCCTATA CGGATTACCC TCAGTTGATG GGGGTGCTGT TTAGGTTTTT GAGAATGGAG 
GCGAATTCGA GTGTCAGGCA AGAGACGATC AAGACGATTG GTATGCTCGG TGCCTTGGAT 
CCCTTTAAGC ACAAGACTTT GTTGGGTGAT GTGGACGACC CTATCGACGA AGGGACAACC 
TCGCGAGTCA ATGACATTGT GTTGCTCAAC CAACACAACT CTTCAGTCAA TGACGAGTTC 
TTCCAGACTG TTGTCATCCA TTCTTTAGTC AACGTCTTGC ATGACTCTAC TTACAAGGAT 
CATTATCAAG CCGTGGAAGC AATTATGATG ATTTTTAGGA CTCAACGATT GAGATGTGTC 
AACTTCCTCC CTCAGATCGT CCCTGCATTC CTCAATGTCA TTCGCATTGC ACATTCCTCT 
CGTACCGAGC TTTACCTCAA ACAACTTGCG CAATTTATTA CAATCGTTAA ACTGCATATC 
CGCAACTACC TCAATGATGT CTTTGATCTA ATCCACGAGT TCTGGAACCC CAACTCTACT 
CTTCAAATTA CCATCATCTC CCTCGTTGAG GCCATCGCGA AGGCTGTGGA AGGTGAATTC 
AAGGCATACT TGCCCAAGCT TTTGCAGCAG ATTTTGAGAT CGTTTGATGG AGACTTGTCA 
GCCAAGCACC TCCCGGAGCT GAAACTCAAC ACTTTGCTTC AGATTCTGAA AGCCTTCTAC 
GTCTTTGGCG AATCTATCGA AGACTACCTC CACCTTGTCT TGCCCGTCAT TGTTCGATCT 
TTTGAGAACC CGGCCGCCCC CGATTCTCTC CGCATTGCTG CTTTGCGCAC TACAGGACAG 
CTTTGTCGTA AAGTCAACTT TTCGGACCAT GCGAGTCAGA TCATCCACCC GTTGGTAAGA 
ACTTTGGGTA ATAGCTCAGA GGAGTTGAGG CAGACTGCAA TGGAAACACT TTGCGTTTTG 
GTGTTGCAGT TCGGCCCAGA TTATGCTATC TTCATTCCTA TGGTAAACAA AGCTTTGGTG 
GAAAACAAAA TATCACATCC TGGTTACGAA GCGCTCATAA CCCAATTGCT CAACCGCGAG 
CGCCTTCCTC CAGATCTTGG CCCTGTCGAA CGATACGCCA GTGACTCTGC CGTCGAAGCC 
TCTGCCCCAG AGCCTGTTGC TCTTAAGGTC AACCAGCAGG CTCTTAAACT GGCCTGGGAC 
TGTTCCCACT TGCTTAACAC CAGCAGCAGG ACCGAGTGGA TCTCTTGGAT TATTGGCTTG 
GGTCATGAGA TGATGAGAGA AAGCCCTAGT CAAGCCATAA GGGCCGCAAG GAGTTTGGCG 
TTGAGTAGTG TGGCCTTCAC CAAGGAACTT TTCAATGTTG CTTTCTACTC ATGCTGGCAG 
GAGCTGTTTG AGAGTTACCA GGAGGATTTA TGGCATAATC TTGACCGAGC AATCAAGAAA 
GATGATGTCC CCGGCGATGT TGTCAATATG ATTCTCGGGG CCACTCAGTT CTTGGAGCAT 
GATGAAAAGG AAGTAGCTAT CGAAAGTCGT GTTTTGGGCA GTGTCGCTGC CAATTATCAA 
GCTCTCGCGG TCGCTTTACA TTACAAAGAA CAGGAATTCT TCCTGGACCC TAGCAAGGAA 
GTCATCGAAG ACCTCATTGA CGTCAACCAG AAACTCCAGC AAAGCGATGC CGCCTGGGGT 
ACCCTTGAAT GGGCTCAGAC AGAGATGGGT ATGACTACTG AGGTCGAGTG GTACGAAAAG 
CTTGGAAGGT GGGAGGAAGC GTTACAAGTG TGGAACGAGC GAGATGCCGA TGCTTCGACC 
ACTTTCTCAG AGTGGGAGAT CACTGAAGGC AAGGTCACTT GTCTGCACGC TATGGGCGAA 
TGGGAACAAC TCTCCGACTT TGTCCAGGCT CGATGGGCCA ACAGGACGGC AGAGGAGAAA 
AAGTTGCTCT CGCCGTTAGC GGCAGCAGCT AGTTGGTCGC TGAAACAATG GGATCTGATG 
GACGATTACA TCTCCGCCAT GAAGGGCGAC GGAGCGGATC GCGCGTTCTT CAAGGCTATC 
TTAGCTGTGC ACAGAAACCA AATCCCTGCT GCTTTGAAGC AAATCTCCAA GGCGCGAGAA 
AGGCTGGATC CAGAGTTGAC TACCTTAACT GGTGACAGCT ACGGCCGAGC GTACGATACT 
GTCGTGAGGA TTCAAATGCT TGCTGAGCTT GAGGAAATCA TTGCCTACAA GGATCACGCC 
GACGAGCCTG CCCGACAGGA GATGCAACGA CAGACTTGGA AGAAGCGTTT GGCTGGATGT 
CAGCGCGATG TCGAAGTTTG GCAGCGCATT CTTCAGGTCA GATCCCTTGT ACTCAAGCCT 
AATGAGGATA TGGACACTTG GATTGAGTTT GCGGACCTCT GTCGAACATC TGACAGGCTG 
AACTTGGCCG AGAAAACGCT GACATCTCTT GTTGGCTTCC AATACCCATC AATGGAAGAC 
ACTCGAGGAC GAGCACCACC TCCCATCATT TTCGCTTACC TACGTATGGC CTGGGCGAAG 
AACCTTCAAA TCGACTCTCG AGAAGAACGT TACGAAACTC TCCAACACTT GCGAGACTTC 
ACGGACCAGC TTACCGACGA TGTTGGTATC GGAGCGAGGG GCCCCAACGG CAGACTCATG 
TTACCTGACC AGAAGTTGTA TGGATCTTAC ACCAAGCTGC TGGCGCAGTG CCATGTTGAA 
TTGGGTCAGT GGCAAGCGAC TTTGAGGGAA AGCCAAGGAT CTGCTGATCC CTCGGGCATT 
CTCCACGACT ATTCTCTCGC CACTGAACTT GACCCTGAGT GGTACCAAGC TTGGCACACT 
TGGGCTTTGG CCAATTTCGA AGTTATTACC CAGCTTGAAG TATCACAGCA AGGTCTCTCA 
CCTATTCACT TTACGACCTA TATCATCCCT GCTGTCGAAG GTTTCCTCAA GTCTATCTCG 
CTTTCTCCTG GCAACTCCTT GCAAGATACT TTAAGGCTGC TGACTCTCTG GTTTACATAT 
GGATACTCTA GCGGAGTGAC AGCGGCCGTT AGCCAAGGTC TGCCCACTGT CAACATTGAT 
GTCTGGCTCG AGGTCATCCC TCAGATTATT GCCCGTATCC AAACGCCACG CCAGTCTATA 
CAGCAGCTCA TTGTACAGCT CTTGCATGAT ATTGGCAAAG CCCATCCTCA AGCCCTTATC 
TACCCTCTTA CCGTCGCCTC CAAATCTACA GTCGCAGCCC GACGCACTGT TGCTCAAAAT 
ATCACCCATA AAATGCGAGA GCACTCTCCC AAGATCGTTG ACCAGGCCGA GCTTGTCAGT 
ACTGAGCTCA TCCGAGCGGC TATCTTATGG CATGAGATGT GGTATGATGG TTTGGAAGAA 
GCGTCAAAGC ACTACTTTGG TGACCATGAT ATCCCTGGCA TGCTGGGAGT TCTTGAACCT 
TTGCATGAGA TTGTCGAAAA CGGACCCCAA ACCTTGCGTG AGACGTCCTT TATTCAATCG 
TTCGGGCATG ATTTGCGTAT CGCCCGAGAG CATCTCAAGC GTTACCGTAT AACTCAGGAT 
GGGACCGAAA TTCAACAAGC ATGGGATGTC TACTACTCCG TCTTCCAGCG TCTCGGCAAA 
CAGCTCAAGC TCCTGAACGT CATTGAGCTG CAATATGTCT CGCCCAAGTT GATGGCCGTT 
CGAGACTTGG ATATTGCTGT TCCAGGTACC TACCAGAGTG GCAAGCCTAT CATCGGTATT 
AAGAACGTTA TCCCGACCTT CAAGGTCATT GCTTCCAAGC AAAAGCCAAG ACAATGCAGC 
ATGCGCGGTA TGGATGGTAA AGAATATGCG TACTGCCTCA AGGGTCACGA GGACTTGCGA 
CAAGACGAGC GTGTTATGCA GCTCTTCGGT TTGGTCAACA CTCTTCTTAA TAATGATCAC 
GAGTCTGCCA AACGACATCT CAGTATCCAG CGATTTTCTG TTACTCCTCT TTCCCCTAGT 
GCCGGTTTAC TCGGTTGGGT TACCCACAGT GATACCATCC ACGTCCTCAT CAAACAATAC 
CGAGACCAAA GGAAAATCTT GGTGGATATT GAGCACAAAC TTATGCAGCA GATGTCTGAT 
GAGAGCTACG ATTCTCTGCC GCTCTTGCAC AAGGTCGAGA TCTTCCAGTA TGCCTTGGAT 
AACACAACTG GTCAGGATTT GTACCGCATC TTGTGGCTCA AGTCTCGTAA CTCAGATATC 
TGGCTTGAGA GAAGGACCAC CTACACTAGA AGTCTGGGTC TCAACTCGAT GGTCGGGTAT 
ATCCTTGGCC TGGGTGACAG ACATCCTTCA AACCTGTTGC TCGATCAGAT TACGGGTAAG 
ATGGTGCACA TTGACTTTGG TGATTGTTTC GAGGTTGCTC AACAGAGAGA CAAGTACCCT 
GAGAAAGTAC CGTTCCGACT TACCCGAATG CTCATTCATG CCATGGAGGT CTGCGGTATC 
ACTGGCAACT TTTCGCGAAG CTGTGAAGTG TCCATGGAAG TCCTTCGTGA CAACAGAGAA 
TCACTTATGG CCGTGCTTGA AGCATTTGTG TACGATCCTC TTATTGCTTG GCGTCTTACG 
GCGACGGACA AACGACCTGG TGGCGTTGGC GAAGTCAAGG ACCTGGATGA CCCGGCGGTG 
TACGGAAAGC AGAGGAAAAA CAAGGCGAAC GAGACAGAGA TTCTCAATGA TGTAGAAAAT 
ACCGAGGTGA AGAATGACAA GGGTCTGCAG GTCATTGAAC GAGTACGACG AAAATTGACC 
GGTCGAGATT TTAAGCCCGA CGTTGTACTC GATGTCAAGT CCCAAGTGGA GAAATTGGTG 
GTTGAGGCGA CAAAGACAGA GAACCTGTGT GTGGCGTTCT TGGGATGGTG TTCCTTCTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_571336.1
CDS	1..7083
Translation	MSSQSDVLDNIFQRLSARSEDVRAQAGQDLAEHVVAYTQEYPGHDASKGVWAQVFHKTFEFTRSNNQLERLGAIISISQLLQLTKDDTLDRAQQKVLRLYEYLRPLTTCGDSTVMLPASLVVEDMVRNSPTLHTDTFLGKEVGQALVMIDDSRQEVGRFSGALLLYAFARAAPGVFHQYIPKVLEKIWVPLRDSRSVVRERASMLLSTCLDTLKTRGDRPSTDTYRKIFEEARLGLLKASSTESILGSLLAFNSMLQNQQLSMAEYYRSICELTFKYRDSKEVSIRKAVIALIPSMATYDSDDFEAHYLHRSMAYLLQALNRPADRDISYVALGHMAVHLGSKMKPFIDDIMRIIRDHLRMRGKKNAPYEAPIFQCLAMLATSVGPMLTRQMHEILNLMFPWGLSEPLCTALQATASHIPPLLRTIQDRLLEMLSQTLTGHSYRPLGAPAPRGGAQMDLNLLQSTTNAQSADTLKLALRLLARFDFVGHTLSEFVRDAALPYLEHDSVEVRREAVLATTTLFMTDPICQQTSSNSVEIVNDVLSKLLTVAITDPNAGIRRTVLDHLEDKFDRHLAQADDIRCLFIALNDEVFGNRERTISIIGRLAHHNPAYVMPHLRKSLINIVTELEYSTNARQKEESAKLLCLIIGAAAGLVKSYAPTILSVLLRTASSPDSSIGVQAECLKCIGELARVAGEELVPSVRAILDLVIEMLNDQASPAKRDTALKTLGQIASNTGEVIKPYTDYPQLMGVLFRFLRMEANSSVRQETIKTIGMLGALDPFKHKTLLGDVDDPIDEGTTSRVNDIVLLNQHNSSVNDEFFQTVVIHSLVNVLHDSTYKDHYQAVEAIMMIFRTQRLRCVNFLPQIVPAFLNVIRIAHSSRTELYLKQLAQFITIVKLHIRNYLNDVFDLIHEFWNPNSTLQITIISLVEAIAKAVEGEFKAYLPKLLQQILRSFDGDLSAKHLPELKLNTLLQILKAFYVFGESIEDYLHLVLPVIVRSFENPAAPDSLRIAALRTTGQLCRKVNFSDHASQIIHPLVRTLGNSSEELRQTAMETLCVLVLQFGPDYAIFIPMVNKALVENKISHPGYEALITQLLNRERLPPDLGPVERYASDSAVEASAPEPVALKVNQQALKLAWDCSHLLNTSSRTEWISWIIGLGHEMMRESPSQAIRAARSLALSSVAFTKELFNVAFYSCWQELFESYQEDLWHNLDRAIKKDDVPGDVVNMILGATQFLEHDEKEVAIESRVLGSVAANYQALAVALHYKEQEFFLDPSKEVIEDLIDVNQKLQQSDAAWGTLEWAQTEMGMTTEVEWYEKLGRWEEALQVWNERDADASTTFSEWEITEGKVTCLHAMGEWEQLSDFVQARWANRTAEEKKLLSPLAAAASWSLKQWDLMDDYISAMKGDGADRAFFKAILAVHRNQIPAALKQISKARERLDPELTTLTGDSYGRAYDTVVRIQMLAELEEIIAYKDHADEPARQEMQRQTWKKRLAGCQRDVEVWQRILQVRSLVLKPNEDMDTWIEFADLCRTSDRLNLAEKTLTSLVGFQYPSMEDTRGRAPPPIIFAYLRMAWAKNLQIDSREERYETLQHLRDFTDQLTDDVGIGARGPNGRLMLPDQKLYGSYTKLLAQCHVELGQWQATLRESQGSADPSGILHDYSLATELDPEWYQAWHTWALANFEVITQLEVSQQGLSPIHFTTYIIPAVEGFLKSISLSPGNSLQDTLRLLTLWFTYGYSSGVTAAVSQGLPTVNIDVWLEVIPQIIARIQTPRQSIQQLIVQLLHDIGKAHPQALIYPLTVASKSTVAARRTVAQNITHKMREHSPKIVDQAELVSTELIRAAILWHEMWYDGLEEASKHYFGDHDIPGMLGVLEPLHEIVENGPQTLRETSFIQSFGHDLRIAREHLKRYRITQDGTEIQQAWDVYYSVFQRLGKQLKLLNVIELQYVSPKLMAVRDLDIAVPGTYQSGKPIIGIKNVIPTFKVIASKQKPRQCSMRGMDGKEYAYCLKGHEDLRQDERVMQLFGLVNTLLNNDHESAKRHLSIQRFSVTPLSPSAGLLGWVTHSDTIHVLIKQYRDQRKILVDIEHKLMQQMSDESYDSLPLLHKVEIFQYALDNTTGQDLYRILWLKSRNSDIWLERRTTYTRSLGLNSMVGYILGLGDRHPSNLLLDQITGKMVHIDFGDCFEVAQQRDKYPEKVPFRLTRMLIHAMEVCGITGNFSRSCEVSMEVLRDNRESLMAVLEAFVYDPLIAWRLTATDKRPGGVGEVKDLDDPAVYGKQRKNKANETEILNDVENTEVKNDKGLQVIERVRRKLTGRDFKPDVVLDVKSQVEKLVVEATKTENLCVAFLGWCSFW

Target ORF information:

RefSeq Version	XM_571336.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 phosphatidylinositol 3-kinase TOR1, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_571336.1

ORF Insert Sequence:

ATGTCTTCAC AATCAGACGT TCTCGACAAC ATCTTTCAGC GTCTGTCTGC CAGGTCGGAG 
GATGTCCGCG CTCAGGCCGG TCAAGATCTT GCAGAGCACG TCGTAGCTTA TACCCAGGAA 
TACCCGGGAC ATGATGCTTC GAAGGGTGTA TGGGCACAGG TCTTTCACAA GACATTCGAG 
TTTACTAGGA GCAACAATCA GCTAGAAAGA TTAGGTGCAA TTATTTCCAT CTCGCAATTG 
TTACAACTAA CGAAGGATGA CACACTGGAT CGTGCTCAAC AAAAGGTTCT ACGCCTCTAT 
GAATATCTTC GGCCTCTCAC AACATGCGGC GATTCGACCG TCATGCTTCC CGCCTCGCTC 
GTCGTTGAAG ATATGGTCCG CAATTCCCCC ACCCTTCACA CCGATACTTT CCTTGGCAAA 
GAAGTTGGAC AAGCGCTGGT AATGATTGAC GACTCACGTC AAGAAGTCGG TCGTTTCAGT 
GGTGCTCTTC TTCTCTATGC TTTCGCCCGT GCTGCCCCCG GAGTATTCCA TCAATACATC 
CCCAAGGTCT TGGAAAAGAT TTGGGTTCCC CTTCGCGACT CAAGATCTGT CGTTCGAGAA 
CGTGCGAGCA TGCTCTTATC TACCTGCCTT GACACTCTCA AAACCCGAGG AGACCGACCA 
TCAACCGACA CCTACCGCAA GATCTTTGAA GAGGCCCGTC TTGGTCTCCT CAAAGCCAGC 
TCAACAGAGT CTATCTTGGG TTCTCTCCTT GCTTTCAATT CCATGCTTCA AAATCAACAG 
CTTTCGATGG CGGAATATTA TCGCTCCATT TGCGAACTAA CCTTCAAATA CCGTGATTCG 
AAGGAAGTTT CCATCAGGAA GGCTGTCATC GCCCTCATAC CTTCTATGGC GACATATGAT 
AGCGACGACT TTGAGGCACA TTATTTGCAT AGGAGTATGG CGTATTTGCT ACAAGCGTTG 
AACAGGCCGG CGGACAGGGA CATTTCGTAT GTGGCATTGG GTCATATGGC CGTACATCTT 
GGATCGAAAA TGAAACCTTT TATTGACGAC ATTATGCGGA TCATTCGAGA TCATCTACGC 
ATGCGAGGCA AAAAAAATGC TCCATACGAA GCCCCCATCT TCCAGTGTCT TGCGATGCTC 
GCTACCTCTG TCGGCCCTAT GCTTACCCGC CAGATGCACG AAATCCTCAA CCTGATGTTC 
CCCTGGGGCC TTTCGGAACC CTTATGTACA GCCCTTCAAG CCACTGCCTC ACATATCCCT 
CCTCTATTGA GGACTATCCA AGATAGGTTG TTGGAGATGC TTTCACAGAC TTTGACCGGA 
CACAGCTATA GACCGTTAGG TGCGCCTGCC CCAAGAGGTG GAGCGCAGAT GGATCTTAAC 
CTTTTGCAAT CTACAACCAA TGCCCAGTCA GCAGACACCC TAAAACTTGC TCTTCGTCTC 
CTTGCGCGCT TTGATTTTGT CGGCCATACC CTTAGCGAGT TTGTCCGTGA CGCTGCTCTT 
CCGTATCTGG AACACGACAG TGTCGAGGTC CGACGTGAAG CCGTGTTGGC GACCACGACC 
CTGTTCATGA CGGACCCGAT CTGTCAACAG ACGAGCAGTA ATTCGGTCGA GATTGTAAAT 
GATGTGTTGA GTAAATTGTT AACCGTAGCC ATTACCGATC CTAATGCTGG AATCCGCCGG 
ACAGTGCTCG ATCACCTCGA AGACAAGTTT GATCGCCATC TCGCTCAGGC TGACGATATC 
CGATGTCTCT TTATCGCTCT CAACGATGAA GTTTTTGGTA ATCGTGAGAG GACTATCTCG 
ATCATTGGGA GATTGGCACA CCATAACCCG GCTTATGTGA TGCCTCATTT GAGAAAGAGT 
TTGATCAATA TTGTCACTGA GTTGGAGTAT TCTACCAATG CGCGACAAAA GGAAGAGAGT 
GCCAAGCTGC TCTGTCTTAT AATCGGCGCT GCCGCAGGTC TCGTCAAGTC TTATGCCCCT 
ACCATCCTAT CCGTTCTTCT CCGCACTGCT TCCAGCCCCG ATTCTTCTAT CGGTGTCCAA 
GCCGAATGCC TCAAATGTAT TGGTGAGCTT GCCAGAGTTG CGGGCGAAGA ACTCGTCCCC 
TCCGTCCGAG CCATTCTCGA CCTCGTCATT GAAATGCTCA ATGACCAGGC TTCCCCCGCC 
AAGAGAGATA CTGCCCTCAA GACTTTAGGG CAGATTGCGA GTAACACAGG AGAAGTTATC 
AAACCCTATA CGGATTACCC TCAGTTGATG GGGGTGCTGT TTAGGTTTTT GAGAATGGAG 
GCGAATTCGA GTGTCAGGCA AGAGACGATC AAGACGATTG GTATGCTCGG TGCCTTGGAT 
CCCTTTAAGC ACAAGACTTT GTTGGGTGAT GTGGACGACC CTATCGACGA AGGGACAACC 
TCGCGAGTCA ATGACATTGT GTTGCTCAAC CAACACAACT CTTCAGTCAA TGACGAGTTC 
TTCCAGACTG TTGTCATCCA TTCTTTAGTC AACGTCTTGC ATGACTCTAC TTACAAGGAT 
CATTATCAAG CCGTGGAAGC AATTATGATG ATTTTTAGGA CTCAACGATT GAGATGTGTC 
AACTTCCTCC CTCAGATCGT CCCTGCATTC CTCAATGTCA TTCGCATTGC ACATTCCTCT 
CGTACCGAGC TTTACCTCAA ACAACTTGCG CAATTTATTA CAATCGTTAA ACTGCATATC 
CGCAACTACC TCAATGATGT CTTTGATCTA ATCCACGAGT TCTGGAACCC CAACTCTACT 
CTTCAAATTA CCATCATCTC CCTCGTTGAG GCCATCGCGA AGGCTGTGGA AGGTGAATTC 
AAGGCATACT TGCCCAAGCT TTTGCAGCAG ATTTTGAGAT CGTTTGATGG AGACTTGTCA 
GCCAAGCACC TCCCGGAGCT GAAACTCAAC ACTTTGCTTC AGATTCTGAA AGCCTTCTAC 
GTCTTTGGCG AATCTATCGA AGACTACCTC CACCTTGTCT TGCCCGTCAT TGTTCGATCT 
TTTGAGAACC CGGCCGCCCC CGATTCTCTC CGCATTGCTG CTTTGCGCAC TACAGGACAG 
CTTTGTCGTA AAGTCAACTT TTCGGACCAT GCGAGTCAGA TCATCCACCC GTTGGTAAGA 
ACTTTGGGTA ATAGCTCAGA GGAGTTGAGG CAGACTGCAA TGGAAACACT TTGCGTTTTG 
GTGTTGCAGT TCGGCCCAGA TTATGCTATC TTCATTCCTA TGGTAAACAA AGCTTTGGTG 
GAAAACAAAA TATCACATCC TGGTTACGAA GCGCTCATAA CCCAATTGCT CAACCGCGAG 
CGCCTTCCTC CAGATCTTGG CCCTGTCGAA CGATACGCCA GTGACTCTGC CGTCGAAGCC 
TCTGCCCCAG AGCCTGTTGC TCTTAAGGTC AACCAGCAGG CTCTTAAACT GGCCTGGGAC 
TGTTCCCACT TGCTTAACAC CAGCAGCAGG ACCGAGTGGA TCTCTTGGAT TATTGGCTTG 
GGTCATGAGA TGATGAGAGA AAGCCCTAGT CAAGCCATAA GGGCCGCAAG GAGTTTGGCG 
TTGAGTAGTG TGGCCTTCAC CAAGGAACTT TTCAATGTTG CTTTCTACTC ATGCTGGCAG 
GAGCTGTTTG AGAGTTACCA GGAGGATTTA TGGCATAATC TTGACCGAGC AATCAAGAAA 
GATGATGTCC CCGGCGATGT TGTCAATATG ATTCTCGGGG CCACTCAGTT CTTGGAGCAT 
GATGAAAAGG AAGTAGCTAT CGAAAGTCGT GTTTTGGGCA GTGTCGCTGC CAATTATCAA 
GCTCTCGCGG TCGCTTTACA TTACAAAGAA CAGGAATTCT TCCTGGACCC TAGCAAGGAA 
GTCATCGAAG ACCTCATTGA CGTCAACCAG AAACTCCAGC AAAGCGATGC CGCCTGGGGT 
ACCCTTGAAT GGGCTCAGAC AGAGATGGGT ATGACTACTG AGGTCGAGTG GTACGAAAAG 
CTTGGAAGGT GGGAGGAAGC GTTACAAGTG TGGAACGAGC GAGATGCCGA TGCTTCGACC 
ACTTTCTCAG AGTGGGAGAT CACTGAAGGC AAGGTCACTT GTCTGCACGC TATGGGCGAA 
TGGGAACAAC TCTCCGACTT TGTCCAGGCT CGATGGGCCA ACAGGACGGC AGAGGAGAAA 
AAGTTGCTCT CGCCGTTAGC GGCAGCAGCT AGTTGGTCGC TGAAACAATG GGATCTGATG 
GACGATTACA TCTCCGCCAT GAAGGGCGAC GGAGCGGATC GCGCGTTCTT CAAGGCTATC 
TTAGCTGTGC ACAGAAACCA AATCCCTGCT GCTTTGAAGC AAATCTCCAA GGCGCGAGAA 
AGGCTGGATC CAGAGTTGAC TACCTTAACT GGTGACAGCT ACGGCCGAGC GTACGATACT 
GTCGTGAGGA TTCAAATGCT TGCTGAGCTT GAGGAAATCA TTGCCTACAA GGATCACGCC 
GACGAGCCTG CCCGACAGGA GATGCAACGA CAGACTTGGA AGAAGCGTTT GGCTGGATGT 
CAGCGCGATG TCGAAGTTTG GCAGCGCATT CTTCAGGTCA GATCCCTTGT ACTCAAGCCT 
AATGAGGATA TGGACACTTG GATTGAGTTT GCGGACCTCT GTCGAACATC TGACAGGCTG 
AACTTGGCCG AGAAAACGCT GACATCTCTT GTTGGCTTCC AATACCCATC AATGGAAGAC 
ACTCGAGGAC GAGCACCACC TCCCATCATT TTCGCTTACC TACGTATGGC CTGGGCGAAG 
AACCTTCAAA TCGACTCTCG AGAAGAACGT TACGAAACTC TCCAACACTT GCGAGACTTC 
ACGGACCAGC TTACCGACGA TGTTGGTATC GGAGCGAGGG GCCCCAACGG CAGACTCATG 
TTACCTGACC AGAAGTTGTA TGGATCTTAC ACCAAGCTGC TGGCGCAGTG CCATGTTGAA 
TTGGGTCAGT GGCAAGCGAC TTTGAGGGAA AGCCAAGGAT CTGCTGATCC CTCGGGCATT 
CTCCACGACT ATTCTCTCGC CACTGAACTT GACCCTGAGT GGTACCAAGC TTGGCACACT 
TGGGCTTTGG CCAATTTCGA AGTTATTACC CAGCTTGAAG TATCACAGCA AGGTCTCTCA 
CCTATTCACT TTACGACCTA TATCATCCCT GCTGTCGAAG GTTTCCTCAA GTCTATCTCG 
CTTTCTCCTG GCAACTCCTT GCAAGATACT TTAAGGCTGC TGACTCTCTG GTTTACATAT 
GGATACTCTA GCGGAGTGAC AGCGGCCGTT AGCCAAGGTC TGCCCACTGT CAACATTGAT 
GTCTGGCTCG AGGTCATCCC TCAGATTATT GCCCGTATCC AAACGCCACG CCAGTCTATA 
CAGCAGCTCA TTGTACAGCT CTTGCATGAT ATTGGCAAAG CCCATCCTCA AGCCCTTATC 
TACCCTCTTA CCGTCGCCTC CAAATCTACA GTCGCAGCCC GACGCACTGT TGCTCAAAAT 
ATCACCCATA AAATGCGAGA GCACTCTCCC AAGATCGTTG ACCAGGCCGA GCTTGTCAGT 
ACTGAGCTCA TCCGAGCGGC TATCTTATGG CATGAGATGT GGTATGATGG TTTGGAAGAA 
GCGTCAAAGC ACTACTTTGG TGACCATGAT ATCCCTGGCA TGCTGGGAGT TCTTGAACCT 
TTGCATGAGA TTGTCGAAAA CGGACCCCAA ACCTTGCGTG AGACGTCCTT TATTCAATCG 
TTCGGGCATG ATTTGCGTAT CGCCCGAGAG CATCTCAAGC GTTACCGTAT AACTCAGGAT 
GGGACCGAAA TTCAACAAGC ATGGGATGTC TACTACTCCG TCTTCCAGCG TCTCGGCAAA 
CAGCTCAAGC TCCTGAACGT CATTGAGCTG CAATATGTCT CGCCCAAGTT GATGGCCGTT 
CGAGACTTGG ATATTGCTGT TCCAGGTACC TACCAGAGTG GCAAGCCTAT CATCGGTATT 
AAGAACGTTA TCCCGACCTT CAAGGTCATT GCTTCCAAGC AAAAGCCAAG ACAATGCAGC 
ATGCGCGGTA TGGATGGTAA AGAATATGCG TACTGCCTCA AGGGTCACGA GGACTTGCGA 
CAAGACGAGC GTGTTATGCA GCTCTTCGGT TTGGTCAACA CTCTTCTTAA TAATGATCAC 
GAGTCTGCCA AACGACATCT CAGTATCCAG CGATTTTCTG TTACTCCTCT TTCCCCTAGT 
GCCGGTTTAC TCGGTTGGGT TACCCACAGT GATACCATCC ACGTCCTCAT CAAACAATAC 
CGAGACCAAA GGAAAATCTT GGTGGATATT GAGCACAAAC TTATGCAGCA GATGTCTGAT 
GAGAGCTACG ATTCTCTGCC GCTCTTGCAC AAGGTCGAGA TCTTCCAGTA TGCCTTGGAT 
AACACAACTG GTCAGGATTT GTACCGCATC TTGTGGCTCA AGTCTCGTAA CTCAGATATC 
TGGCTTGAGA GAAGGACCAC CTACACTAGA AGTCTGGGTC TCAACTCGAT GGTCGGGTAT 
ATCCTTGGCC TGGGTGACAG ACATCCTTCA AACCTGTTGC TCGATCAGAT TACGGGTAAG 
ATGGTGCACA TTGACTTTGG TGATTGTTTC GAGGTTGCTC AACAGAGAGA CAAGTACCCT 
GAGAAAGTAC CGTTCCGACT TACCCGAATG CTCATTCATG CCATGGAGGT CTGCGGTATC 
ACTGGCAACT TTTCGCGAAG CTGTGAAGTG TCCATGGAAG TCCTTCGTGA CAACAGAGAA 
TCACTTATGG CCGTGCTTGA AGCATTTGTG TACGATCCTC TTATTGCTTG GCGTCTTACG 
GCGACGGACA AACGACCTGG TGGCGTTGGC GAAGTCAAGG ACCTGGATGA CCCGGCGGTG 
TACGGAAAGC AGAGGAAAAA CAAGGCGAAC GAGACAGAGA TTCTCAATGA TGTAGAAAAT 
ACCGAGGTGA AGAATGACAA GGGTCTGCAG GTCATTGAAC GAGTACGACG AAAATTGACC 
GGTCGAGATT TTAAGCCCGA CGTTGTACTC GATGTCAAGT CCCAAGTGGA GAAATTGGTG 
GTTGAGGCGA CAAAGACAGA GAACCTGTGT GTGGCGTTCT TGGGATGGTG TTCCTTCTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW

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CNF03740 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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