TIE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIE1
Entrez Gene ID	482535
Full Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
General protein information	Preferred Names tyrosine kinase with immunoglobulin like and EGF like domains 1 Names tyrosine-protein kinase receptor Tie-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_539652.5	XP_539652.3	tyrosine-protein kinase receptor Tie-1
XM_539652.6	XP_539652.3	tyrosine-protein kinase receptor Tie-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TIE1	NP_776390.1
Rattus norvegicus (Norway rat)	Tie1	NP_445997.1
Gallus gallus (chicken)	TIE1	XP_004936746.1
Danio rerio (zebrafish)	tie1	XP_001334673.5
Pan troglodytes (chimpanzee)	TIE1	XP_001173341.1
Canis lupus familiaris (dog)	TIE1	XP_539652.3
Mus musculus (house mouse)	Tie1	NP_035717.2
Xenopus tropicalis (tropical clawed frog)	tie1	XP_002931650.2
Homo sapiens (human)	TIE1	NP_005415.1

Gene Ontology

The following TIE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe17438	XM_539652.6 Latest version!	Canis lupus familiaris tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe17438	XM_539652.5	Canis lupus familiaris tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIE1 ( XM_539652.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539652.3 Canis lupus familiaris tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17438
Clone ID Related Accession (Same CDS sequence)	XM_539652.5 , XM_539652.6
Accession Version	XM_539652.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	tyrosine-protein kinase receptor Tie-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539652.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC TGGGGCTCCC TTTGCTGCTC CCCATCCTCT TTCTGGCTTC TCATGTTGGC 
GCCGCGGTGG ACCTGACGCT GCTGGCCGAC CTGCGGCTCG TGGAGCCCCA GCGCTTCTTC 
CTGACCTGCG TGTCCGGGGA GGCCGGGCCG GGCCGGGGCG CCGAGGCCTG GGCGCCGCCG 
CTGCTGCTGG AGAAGGACGA CCGCATCGTG CGCACGCCGC CCGGGCAGCC CCCGCACCTG 
CTGCGCAACG GCTCGCACAG CGTCACGCTG CGGGGCTTCT CGCAGCCCTC CGACCTCGTG 
GGCGTCTTCT CGTGCGTGGG CGGCGCGGGC CCGGGGCGCA CCCGCGTCCT CTACGTGCAC 
AACAGCCCCG GAGCGCACCT GCTCCCGGAC AAGGTCACGC ACACGGTGAA CAAGGGCGAC 
ACGGCCGTGC TTGCGGCGCG TGTGCACAAG GAGAAGCAGA CGGATGTGAT CTGGAAAAGC 
AACGGATCCT ACTTCTATAC CCTGGACTGG CATGAAGCCC AGGACGGGCA CTTCCTGCTG 
CAGCTCCCCA ACGTGCAGCC ACCTTCGAGT GGCATCTACA GTGCCACCTA CCTGGAAGCC 
AGCCCCCTAG GCAGCGCCTT CTTCAGGCTC ATTGTGCGGG GCTGTGGGGC AGGGCGCTGG 
GGACCAGACT GTACCAAGGA ATGCCCCGGC TGCCTGCACG GAGGTGTCTG CCATGACCAG 
GATGGCGAGT GTGTGTGTCC CCCTGGATTC ACTGGTACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCACGTCAGG CTGCCGGGGT 
CTCACCTTCT GCCTCCCAGA CCCCTACGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAGG AAGCCTGTGC CCCTGGCCAC TTCGGGGCAG ATTGCCGCCT CCAGTGCCAC 
TGTCGGAACG GTGGTACTTG TGACCGCTTC AGTGGCTGCG TCTGTCCCTC CGGGTGGCAC 
GGAATGCACT GTGAGAAGTC AGACCGGATC CCGCAGATCC TGGACTTGGC CTCGGAACTG 
GAGTTCAACT TAGAGACCAT GCCCCGGATC AACTGTGCAG CCGCGGGGAA CCCCTTCCCG 
GTGCGGGGCA GCATGGAGCT CCGGAAGCCC GACGGCACGG TCCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGACAGGAC CACAGCCGAG TTCGAGGTGC CCCGCTTGAC CCTTGGGGAC 
AGCGGGCTCT GGGAGTGCCG CGTGTCCACG TCTGGTGGCC AAGACAGCCG ACGTTTCAGG 
GTCAACGTCA AAGTGCCCCC GGTGCCCCTG ACCCCTCCTC GGCTCCTGGC CAAGCACAGC 
CGCCAGCTCG TGGTCTCGCC GCTGGTCTCC TTCTCCGGGG ACGGACCCAT CGCCTCCGTC 
CGCCTGCACT ACCGGCCCCA GGACAGCACC ATGGCCTGGT CGGCGATTGT GGTGGACCCC 
AGTGAGAATG TGACTTTAAT GAACCTGAGG CCGAAGACAG GATACAGCGT CCGAGTGCAG 
CTGAGCCGGC CGGGGGAGGG CGGGGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACG 
GACTGTCCCG AGCCCTCACT GCAGCCGTGG CTGGAGGGCT GGCACGTGGA GGGCCCTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTCG GTGCCCGGGC CACTGGTGGG CGATGGTTTC 
CTGCTGCGCC TATGGGATGG GGCACGGGGA CAGGAGCGGC GGGAGAACGT GTCGTCCCCC 
CAGGCCCGCA CCGCCCTCCT GACGGGACTC GCGCCGGGCA CCCAGTACCA GCTGGACGTG 
CGGCTGTACC ACTGCACCCT CCTGGGCCCC GCCTCGCCCC CTGCGCGCGT GCTTCTGCCC 
CCCAGCGGGC CCCCAGCCCC CCGCTACCTC CGTGCCCAGG CCCTCTCAGA CTCAGAGATC 
CAGCTGACGT GGCTGCGCCC AGAGGCTCCG GCTGGGCCTA TATCCAAGTA CATCGTGGAG 
GTGCAGGTGG CGGGGGGCTC GGGAGACCCG CTGTGGATGG ACGTGGACAG GCCCGAGGAG 
ACCAGCACGG TTGTCCGTGG CCTTAACGCC AGCACACGCT ACCTCTTCCG TGTGCGGGCC 
AGTATCCAGG GTCCCGGAGA CTGGAGCACT GTGGTGGAGG AGTCCACCCT GGGCAATGGT 
CTGCAGAGTG AGGGCCCGGT CCAAGAAGTC CAGGCGGCTG AGGAGGGCCT GGACCGGCAG 
CTGATCCTGG CTGTAGTAGG CTCTGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTG 
CTAACCCTCG TGTGCATCCG CAGAAGCTGC CTGCACCGCA GACGCACCTT CACCTACCAG 
TCTGGCTCGG GTGAGGAAAC CATCCTGCAG TTCAGCTCAG GAACCTTGAC GCTAACCCGG 
CGGCCGAAAC CACAGCCCGA GCCCCTGAGT TATCCCGTGC TGGAGTGGGA AGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTT GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTCA AGATGAATGC GGCCATCAAG ATGCTGAAAG AGTACGCCTC TGAAAATGAC 
CATCGTGACT TCGCAGGAGA ACTGGAAGTT CTGTGCAAAC TGGGGCATCA CCCCAATATC 
ATCAACCTCT TGGGGGCTTG TGAGAACCGA GGTTACCTGT ATATCGCTAT TGAATACGCC 
CCCTACGGGA ACCTGCTAGA CTTTCTGCGG AAGAGCCGGG TCTTGGAGAC GGACCCAGCT 
TTCGCCCGAG AGCATGGCAC GGCCTCCACC CTCAGCTCCC GGCAGCTGCT GCGTTTTGCC 
AGCGATGCCG CCAACGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGAGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTGGCCTCCA AGATTGCTGA CTTCGGCCTT 
TCTCGGGGGG AGGAGGTTTA TGTGAAGAAG ACAATGGGGC GTCTCCCCGT GCGCTGGATG 
GCCATTGAGT CTCTGAATTA CAGCGTCTAT ACCACCAAGA GTGATGTTTG GTCCTTTGGG 
GTCCTCCTCT GGGAGATCGT GAGCCTTGGG GGCACACCTT ACTGTGGCAT GACCTGTGCT 
GAGCTCTATG AGAAGCTGCC TCAGGGCTAC CGCATGGAAC AGCCTCGAAA CTGTGATGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CGTACGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTGCAGCT GGGCCGGATG CTGGAAGCCA GGAAGGCCTA CGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATCGACGCCA CAGCCGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539652.3
CDS	88..3504
Translation	MVWLGLPLLLPILFLASHVGAAVDLTLLADLRLVEPQRFFLTCVSGEAGPGRGAEAWAPPLLLEKDDRIVRTPPGQPPHLLRNGSHSVTLRGFSQPSDLVGVFSCVGGAGPGRTRVLYVHNSPGAHLLPDKVTHTVNKGDTAVLAARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGHFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPDCTKECPGCLHGGVCHDQDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTSGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCHCRNGGTCDRFSGCVCPSGWHGMHCEKSDRIPQILDLASELEFNLETMPRINCAAAGNPFPVRGSMELRKPDGTVLLSTKAIVEPDRTTAEFEVPRLTLGDSGLWECRVSTSGGQDSRRFRVNVKVPPVPLTPPRLLAKHSRQLVVSPLVSFSGDGPIASVRLHYRPQDSTMAWSAIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPSLQPWLEGWHVEGPDRLRVSWSLPSVPGPLVGDGFLLRLWDGARGQERRENVSSPQARTALLTGLAPGTQYQLDVRLYHCTLLGPASPPARVLLPPSGPPAPRYLRAQALSDSEIQLTWLRPEAPAGPISKYIVEVQVAGGSGDPLWMDVDRPEETSTVVRGLNASTRYLFRVRASIQGPGDWSTVVEESTLGNGLQSEGPVQEVQAAEEGLDRQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_539652.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539652.5

ORF Insert Sequence:

ATGGTCTGGC TGGGGCTCCC TTTGCTGCTC CCCATCCTCT TTCTGGCTTC TCATGTTGGC 
GCCGCGGTGG ACCTGACGCT GCTGGCCGAC CTGCGGCTCG TGGAGCCCCA GCGCTTCTTC 
CTGACCTGCG TGTCCGGGGA GGCCGGGCCG GGCCGGGGCG CCGAGGCCTG GGCGCCGCCG 
CTGCTGCTGG AGAAGGACGA CCGCATCGTG CGCACGCCGC CCGGGCAGCC CCCGCACCTG 
CTGCGCAACG GCTCGCACAG CGTCACGCTG CGGGGCTTCT CGCAGCCCTC CGACCTCGTG 
GGCGTCTTCT CGTGCGTGGG CGGCGCGGGC CCGGGGCGCA CCCGCGTCCT CTACGTGCAC 
AACAGCCCCG GAGCGCACCT GCTCCCGGAC AAGGTCACGC ACACGGTGAA CAAGGGCGAC 
ACGGCCGTGC TTGCGGCGCG TGTGCACAAG GAGAAGCAGA CGGATGTGAT CTGGAAAAGC 
AACGGATCCT ACTTCTATAC CCTGGACTGG CATGAAGCCC AGGACGGGCA CTTCCTGCTG 
CAGCTCCCCA ACGTGCAGCC ACCTTCGAGT GGCATCTACA GTGCCACCTA CCTGGAAGCC 
AGCCCCCTAG GCAGCGCCTT CTTCAGGCTC ATTGTGCGGG GCTGTGGGGC AGGGCGCTGG 
GGACCAGACT GTACCAAGGA ATGCCCCGGC TGCCTGCACG GAGGTGTCTG CCATGACCAG 
GATGGCGAGT GTGTGTGTCC CCCTGGATTC ACTGGTACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCACGTCAGG CTGCCGGGGT 
CTCACCTTCT GCCTCCCAGA CCCCTACGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAGG AAGCCTGTGC CCCTGGCCAC TTCGGGGCAG ATTGCCGCCT CCAGTGCCAC 
TGTCGGAACG GTGGTACTTG TGACCGCTTC AGTGGCTGCG TCTGTCCCTC CGGGTGGCAC 
GGAATGCACT GTGAGAAGTC AGACCGGATC CCGCAGATCC TGGACTTGGC CTCGGAACTG 
GAGTTCAACT TAGAGACCAT GCCCCGGATC AACTGTGCAG CCGCGGGGAA CCCCTTCCCG 
GTGCGGGGCA GCATGGAGCT CCGGAAGCCC GACGGCACGG TCCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGACAGGAC CACAGCCGAG TTCGAGGTGC CCCGCTTGAC CCTTGGGGAC 
AGCGGGCTCT GGGAGTGCCG CGTGTCCACG TCTGGTGGCC AAGACAGCCG ACGTTTCAGG 
GTCAACGTCA AAGTGCCCCC GGTGCCCCTG ACCCCTCCTC GGCTCCTGGC CAAGCACAGC 
CGCCAGCTCG TGGTCTCGCC GCTGGTCTCC TTCTCCGGGG ACGGACCCAT CGCCTCCGTC 
CGCCTGCACT ACCGGCCCCA GGACAGCACC ATGGCCTGGT CGGCGATTGT GGTGGACCCC 
AGTGAGAATG TGACTTTAAT GAACCTGAGG CCGAAGACAG GATACAGCGT CCGAGTGCAG 
CTGAGCCGGC CGGGGGAGGG CGGGGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACG 
GACTGTCCCG AGCCCTCACT GCAGCCGTGG CTGGAGGGCT GGCACGTGGA GGGCCCTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTCG GTGCCCGGGC CACTGGTGGG CGATGGTTTC 
CTGCTGCGCC TATGGGATGG GGCACGGGGA CAGGAGCGGC GGGAGAACGT GTCGTCCCCC 
CAGGCCCGCA CCGCCCTCCT GACGGGACTC GCGCCGGGCA CCCAGTACCA GCTGGACGTG 
CGGCTGTACC ACTGCACCCT CCTGGGCCCC GCCTCGCCCC CTGCGCGCGT GCTTCTGCCC 
CCCAGCGGGC CCCCAGCCCC CCGCTACCTC CGTGCCCAGG CCCTCTCAGA CTCAGAGATC 
CAGCTGACGT GGCTGCGCCC AGAGGCTCCG GCTGGGCCTA TATCCAAGTA CATCGTGGAG 
GTGCAGGTGG CGGGGGGCTC GGGAGACCCG CTGTGGATGG ACGTGGACAG GCCCGAGGAG 
ACCAGCACGG TTGTCCGTGG CCTTAACGCC AGCACACGCT ACCTCTTCCG TGTGCGGGCC 
AGTATCCAGG GTCCCGGAGA CTGGAGCACT GTGGTGGAGG AGTCCACCCT GGGCAATGGT 
CTGCAGAGTG AGGGCCCGGT CCAAGAAGTC CAGGCGGCTG AGGAGGGCCT GGACCGGCAG 
CTGATCCTGG CTGTAGTAGG CTCTGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTG 
CTAACCCTCG TGTGCATCCG CAGAAGCTGC CTGCACCGCA GACGCACCTT CACCTACCAG 
TCTGGCTCGG GTGAGGAAAC CATCCTGCAG TTCAGCTCAG GAACCTTGAC GCTAACCCGG 
CGGCCGAAAC CACAGCCCGA GCCCCTGAGT TATCCCGTGC TGGAGTGGGA AGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTT GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTCA AGATGAATGC GGCCATCAAG ATGCTGAAAG AGTACGCCTC TGAAAATGAC 
CATCGTGACT TCGCAGGAGA ACTGGAAGTT CTGTGCAAAC TGGGGCATCA CCCCAATATC 
ATCAACCTCT TGGGGGCTTG TGAGAACCGA GGTTACCTGT ATATCGCTAT TGAATACGCC 
CCCTACGGGA ACCTGCTAGA CTTTCTGCGG AAGAGCCGGG TCTTGGAGAC GGACCCAGCT 
TTCGCCCGAG AGCATGGCAC GGCCTCCACC CTCAGCTCCC GGCAGCTGCT GCGTTTTGCC 
AGCGATGCCG CCAACGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGAGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTGGCCTCCA AGATTGCTGA CTTCGGCCTT 
TCTCGGGGGG AGGAGGTTTA TGTGAAGAAG ACAATGGGGC GTCTCCCCGT GCGCTGGATG 
GCCATTGAGT CTCTGAATTA CAGCGTCTAT ACCACCAAGA GTGATGTTTG GTCCTTTGGG 
GTCCTCCTCT GGGAGATCGT GAGCCTTGGG GGCACACCTT ACTGTGGCAT GACCTGTGCT 
GAGCTCTATG AGAAGCTGCC TCAGGGCTAC CGCATGGAAC AGCCTCGAAA CTGTGATGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CGTACGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTGCAGCT GGGCCGGATG CTGGAAGCCA GGAAGGCCTA CGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATCGACGCCA CAGCCGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_539652.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539652.3 Canis lupus familiaris tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17438
Clone ID Related Accession (Same CDS sequence)	XM_539652.5 , XM_539652.6
Accession Version	XM_539652.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	tyrosine-protein kinase receptor Tie-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539652.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC TGGGGCTCCC TTTGCTGCTC CCCATCCTCT TTCTGGCTTC TCATGTTGGC 
GCCGCGGTGG ACCTGACGCT GCTGGCCGAC CTGCGGCTCG TGGAGCCCCA GCGCTTCTTC 
CTGACCTGCG TGTCCGGGGA GGCCGGGCCG GGCCGGGGCG CCGAGGCCTG GGCGCCGCCG 
CTGCTGCTGG AGAAGGACGA CCGCATCGTG CGCACGCCGC CCGGGCAGCC CCCGCACCTG 
CTGCGCAACG GCTCGCACAG CGTCACGCTG CGGGGCTTCT CGCAGCCCTC CGACCTCGTG 
GGCGTCTTCT CGTGCGTGGG CGGCGCGGGC CCGGGGCGCA CCCGCGTCCT CTACGTGCAC 
AACAGCCCCG GAGCGCACCT GCTCCCGGAC AAGGTCACGC ACACGGTGAA CAAGGGCGAC 
ACGGCCGTGC TTGCGGCGCG TGTGCACAAG GAGAAGCAGA CGGATGTGAT CTGGAAAAGC 
AACGGATCCT ACTTCTATAC CCTGGACTGG CATGAAGCCC AGGACGGGCA CTTCCTGCTG 
CAGCTCCCCA ACGTGCAGCC ACCTTCGAGT GGCATCTACA GTGCCACCTA CCTGGAAGCC 
AGCCCCCTAG GCAGCGCCTT CTTCAGGCTC ATTGTGCGGG GCTGTGGGGC AGGGCGCTGG 
GGACCAGACT GTACCAAGGA ATGCCCCGGC TGCCTGCACG GAGGTGTCTG CCATGACCAG 
GATGGCGAGT GTGTGTGTCC CCCTGGATTC ACTGGTACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCACGTCAGG CTGCCGGGGT 
CTCACCTTCT GCCTCCCAGA CCCCTACGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAGG AAGCCTGTGC CCCTGGCCAC TTCGGGGCAG ATTGCCGCCT CCAGTGCCAC 
TGTCGGAACG GTGGTACTTG TGACCGCTTC AGTGGCTGCG TCTGTCCCTC CGGGTGGCAC 
GGAATGCACT GTGAGAAGTC AGACCGGATC CCGCAGATCC TGGACTTGGC CTCGGAACTG 
GAGTTCAACT TAGAGACCAT GCCCCGGATC AACTGTGCAG CCGCGGGGAA CCCCTTCCCG 
GTGCGGGGCA GCATGGAGCT CCGGAAGCCC GACGGCACGG TCCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGACAGGAC CACAGCCGAG TTCGAGGTGC CCCGCTTGAC CCTTGGGGAC 
AGCGGGCTCT GGGAGTGCCG CGTGTCCACG TCTGGTGGCC AAGACAGCCG ACGTTTCAGG 
GTCAACGTCA AAGTGCCCCC GGTGCCCCTG ACCCCTCCTC GGCTCCTGGC CAAGCACAGC 
CGCCAGCTCG TGGTCTCGCC GCTGGTCTCC TTCTCCGGGG ACGGACCCAT CGCCTCCGTC 
CGCCTGCACT ACCGGCCCCA GGACAGCACC ATGGCCTGGT CGGCGATTGT GGTGGACCCC 
AGTGAGAATG TGACTTTAAT GAACCTGAGG CCGAAGACAG GATACAGCGT CCGAGTGCAG 
CTGAGCCGGC CGGGGGAGGG CGGGGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACG 
GACTGTCCCG AGCCCTCACT GCAGCCGTGG CTGGAGGGCT GGCACGTGGA GGGCCCTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTCG GTGCCCGGGC CACTGGTGGG CGATGGTTTC 
CTGCTGCGCC TATGGGATGG GGCACGGGGA CAGGAGCGGC GGGAGAACGT GTCGTCCCCC 
CAGGCCCGCA CCGCCCTCCT GACGGGACTC GCGCCGGGCA CCCAGTACCA GCTGGACGTG 
CGGCTGTACC ACTGCACCCT CCTGGGCCCC GCCTCGCCCC CTGCGCGCGT GCTTCTGCCC 
CCCAGCGGGC CCCCAGCCCC CCGCTACCTC CGTGCCCAGG CCCTCTCAGA CTCAGAGATC 
CAGCTGACGT GGCTGCGCCC AGAGGCTCCG GCTGGGCCTA TATCCAAGTA CATCGTGGAG 
GTGCAGGTGG CGGGGGGCTC GGGAGACCCG CTGTGGATGG ACGTGGACAG GCCCGAGGAG 
ACCAGCACGG TTGTCCGTGG CCTTAACGCC AGCACACGCT ACCTCTTCCG TGTGCGGGCC 
AGTATCCAGG GTCCCGGAGA CTGGAGCACT GTGGTGGAGG AGTCCACCCT GGGCAATGGT 
CTGCAGAGTG AGGGCCCGGT CCAAGAAGTC CAGGCGGCTG AGGAGGGCCT GGACCGGCAG 
CTGATCCTGG CTGTAGTAGG CTCTGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTG 
CTAACCCTCG TGTGCATCCG CAGAAGCTGC CTGCACCGCA GACGCACCTT CACCTACCAG 
TCTGGCTCGG GTGAGGAAAC CATCCTGCAG TTCAGCTCAG GAACCTTGAC GCTAACCCGG 
CGGCCGAAAC CACAGCCCGA GCCCCTGAGT TATCCCGTGC TGGAGTGGGA AGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTT GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTCA AGATGAATGC GGCCATCAAG ATGCTGAAAG AGTACGCCTC TGAAAATGAC 
CATCGTGACT TCGCAGGAGA ACTGGAAGTT CTGTGCAAAC TGGGGCATCA CCCCAATATC 
ATCAACCTCT TGGGGGCTTG TGAGAACCGA GGTTACCTGT ATATCGCTAT TGAATACGCC 
CCCTACGGGA ACCTGCTAGA CTTTCTGCGG AAGAGCCGGG TCTTGGAGAC GGACCCAGCT 
TTCGCCCGAG AGCATGGCAC GGCCTCCACC CTCAGCTCCC GGCAGCTGCT GCGTTTTGCC 
AGCGATGCCG CCAACGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGAGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTGGCCTCCA AGATTGCTGA CTTCGGCCTT 
TCTCGGGGGG AGGAGGTTTA TGTGAAGAAG ACAATGGGGC GTCTCCCCGT GCGCTGGATG 
GCCATTGAGT CTCTGAATTA CAGCGTCTAT ACCACCAAGA GTGATGTTTG GTCCTTTGGG 
GTCCTCCTCT GGGAGATCGT GAGCCTTGGG GGCACACCTT ACTGTGGCAT GACCTGTGCT 
GAGCTCTATG AGAAGCTGCC TCAGGGCTAC CGCATGGAAC AGCCTCGAAA CTGTGATGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CGTACGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTGCAGCT GGGCCGGATG CTGGAAGCCA GGAAGGCCTA CGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATCGACGCCA CAGCCGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539652.3
CDS	92..3508
Translation	MVWLGLPLLLPILFLASHVGAAVDLTLLADLRLVEPQRFFLTCVSGEAGPGRGAEAWAPPLLLEKDDRIVRTPPGQPPHLLRNGSHSVTLRGFSQPSDLVGVFSCVGGAGPGRTRVLYVHNSPGAHLLPDKVTHTVNKGDTAVLAARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGHFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPDCTKECPGCLHGGVCHDQDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTSGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCHCRNGGTCDRFSGCVCPSGWHGMHCEKSDRIPQILDLASELEFNLETMPRINCAAAGNPFPVRGSMELRKPDGTVLLSTKAIVEPDRTTAEFEVPRLTLGDSGLWECRVSTSGGQDSRRFRVNVKVPPVPLTPPRLLAKHSRQLVVSPLVSFSGDGPIASVRLHYRPQDSTMAWSAIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPSLQPWLEGWHVEGPDRLRVSWSLPSVPGPLVGDGFLLRLWDGARGQERRENVSSPQARTALLTGLAPGTQYQLDVRLYHCTLLGPASPPARVLLPPSGPPAPRYLRAQALSDSEIQLTWLRPEAPAGPISKYIVEVQVAGGSGDPLWMDVDRPEETSTVVRGLNASTRYLFRVRASIQGPGDWSTVVEESTLGNGLQSEGPVQEVQAAEEGLDRQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_539652.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539652.6

ORF Insert Sequence:

ATGGTCTGGC TGGGGCTCCC TTTGCTGCTC CCCATCCTCT TTCTGGCTTC TCATGTTGGC 
GCCGCGGTGG ACCTGACGCT GCTGGCCGAC CTGCGGCTCG TGGAGCCCCA GCGCTTCTTC 
CTGACCTGCG TGTCCGGGGA GGCCGGGCCG GGCCGGGGCG CCGAGGCCTG GGCGCCGCCG 
CTGCTGCTGG AGAAGGACGA CCGCATCGTG CGCACGCCGC CCGGGCAGCC CCCGCACCTG 
CTGCGCAACG GCTCGCACAG CGTCACGCTG CGGGGCTTCT CGCAGCCCTC CGACCTCGTG 
GGCGTCTTCT CGTGCGTGGG CGGCGCGGGC CCGGGGCGCA CCCGCGTCCT CTACGTGCAC 
AACAGCCCCG GAGCGCACCT GCTCCCGGAC AAGGTCACGC ACACGGTGAA CAAGGGCGAC 
ACGGCCGTGC TTGCGGCGCG TGTGCACAAG GAGAAGCAGA CGGATGTGAT CTGGAAAAGC 
AACGGATCCT ACTTCTATAC CCTGGACTGG CATGAAGCCC AGGACGGGCA CTTCCTGCTG 
CAGCTCCCCA ACGTGCAGCC ACCTTCGAGT GGCATCTACA GTGCCACCTA CCTGGAAGCC 
AGCCCCCTAG GCAGCGCCTT CTTCAGGCTC ATTGTGCGGG GCTGTGGGGC AGGGCGCTGG 
GGACCAGACT GTACCAAGGA ATGCCCCGGC TGCCTGCACG GAGGTGTCTG CCATGACCAG 
GATGGCGAGT GTGTGTGTCC CCCTGGATTC ACTGGTACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCACGTCAGG CTGCCGGGGT 
CTCACCTTCT GCCTCCCAGA CCCCTACGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAGG AAGCCTGTGC CCCTGGCCAC TTCGGGGCAG ATTGCCGCCT CCAGTGCCAC 
TGTCGGAACG GTGGTACTTG TGACCGCTTC AGTGGCTGCG TCTGTCCCTC CGGGTGGCAC 
GGAATGCACT GTGAGAAGTC AGACCGGATC CCGCAGATCC TGGACTTGGC CTCGGAACTG 
GAGTTCAACT TAGAGACCAT GCCCCGGATC AACTGTGCAG CCGCGGGGAA CCCCTTCCCG 
GTGCGGGGCA GCATGGAGCT CCGGAAGCCC GACGGCACGG TCCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGACAGGAC CACAGCCGAG TTCGAGGTGC CCCGCTTGAC CCTTGGGGAC 
AGCGGGCTCT GGGAGTGCCG CGTGTCCACG TCTGGTGGCC AAGACAGCCG ACGTTTCAGG 
GTCAACGTCA AAGTGCCCCC GGTGCCCCTG ACCCCTCCTC GGCTCCTGGC CAAGCACAGC 
CGCCAGCTCG TGGTCTCGCC GCTGGTCTCC TTCTCCGGGG ACGGACCCAT CGCCTCCGTC 
CGCCTGCACT ACCGGCCCCA GGACAGCACC ATGGCCTGGT CGGCGATTGT GGTGGACCCC 
AGTGAGAATG TGACTTTAAT GAACCTGAGG CCGAAGACAG GATACAGCGT CCGAGTGCAG 
CTGAGCCGGC CGGGGGAGGG CGGGGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACG 
GACTGTCCCG AGCCCTCACT GCAGCCGTGG CTGGAGGGCT GGCACGTGGA GGGCCCTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTCG GTGCCCGGGC CACTGGTGGG CGATGGTTTC 
CTGCTGCGCC TATGGGATGG GGCACGGGGA CAGGAGCGGC GGGAGAACGT GTCGTCCCCC 
CAGGCCCGCA CCGCCCTCCT GACGGGACTC GCGCCGGGCA CCCAGTACCA GCTGGACGTG 
CGGCTGTACC ACTGCACCCT CCTGGGCCCC GCCTCGCCCC CTGCGCGCGT GCTTCTGCCC 
CCCAGCGGGC CCCCAGCCCC CCGCTACCTC CGTGCCCAGG CCCTCTCAGA CTCAGAGATC 
CAGCTGACGT GGCTGCGCCC AGAGGCTCCG GCTGGGCCTA TATCCAAGTA CATCGTGGAG 
GTGCAGGTGG CGGGGGGCTC GGGAGACCCG CTGTGGATGG ACGTGGACAG GCCCGAGGAG 
ACCAGCACGG TTGTCCGTGG CCTTAACGCC AGCACACGCT ACCTCTTCCG TGTGCGGGCC 
AGTATCCAGG GTCCCGGAGA CTGGAGCACT GTGGTGGAGG AGTCCACCCT GGGCAATGGT 
CTGCAGAGTG AGGGCCCGGT CCAAGAAGTC CAGGCGGCTG AGGAGGGCCT GGACCGGCAG 
CTGATCCTGG CTGTAGTAGG CTCTGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTG 
CTAACCCTCG TGTGCATCCG CAGAAGCTGC CTGCACCGCA GACGCACCTT CACCTACCAG 
TCTGGCTCGG GTGAGGAAAC CATCCTGCAG TTCAGCTCAG GAACCTTGAC GCTAACCCGG 
CGGCCGAAAC CACAGCCCGA GCCCCTGAGT TATCCCGTGC TGGAGTGGGA AGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTT GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTCA AGATGAATGC GGCCATCAAG ATGCTGAAAG AGTACGCCTC TGAAAATGAC 
CATCGTGACT TCGCAGGAGA ACTGGAAGTT CTGTGCAAAC TGGGGCATCA CCCCAATATC 
ATCAACCTCT TGGGGGCTTG TGAGAACCGA GGTTACCTGT ATATCGCTAT TGAATACGCC 
CCCTACGGGA ACCTGCTAGA CTTTCTGCGG AAGAGCCGGG TCTTGGAGAC GGACCCAGCT 
TTCGCCCGAG AGCATGGCAC GGCCTCCACC CTCAGCTCCC GGCAGCTGCT GCGTTTTGCC 
AGCGATGCCG CCAACGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGAGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTGGCCTCCA AGATTGCTGA CTTCGGCCTT 
TCTCGGGGGG AGGAGGTTTA TGTGAAGAAG ACAATGGGGC GTCTCCCCGT GCGCTGGATG 
GCCATTGAGT CTCTGAATTA CAGCGTCTAT ACCACCAAGA GTGATGTTTG GTCCTTTGGG 
GTCCTCCTCT GGGAGATCGT GAGCCTTGGG GGCACACCTT ACTGTGGCAT GACCTGTGCT 
GAGCTCTATG AGAAGCTGCC TCAGGGCTAC CGCATGGAAC AGCCTCGAAA CTGTGATGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CGTACGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTGCAGCT GGGCCGGATG CTGGAAGCCA GGAAGGCCTA CGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATCGACGCCA CAGCCGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TIE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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