BRAFLDRAFT_124682 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_124682
Entrez Gene ID	7243539
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002603766.1	XP_002603812.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_124682 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_124682 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb16307	XM_002603766.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_124682 ( XM_002603766.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002603812.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb16307
Clone ID Related Accession (Same CDS sequence)	XM_002603766.1
Accession Version	XM_002603766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101505). COMPLETENESS: incomplete on the 5' end.

ATGGCGGACG CATCGGCTCG TTCGCTTCAG TACGAGTACA AAGCGAACTC CAACCTTGTT 
CTCCAAGCCG ACCGGTCGCT GATTGACCGC AGAAGTCGCG ATGAGGCGAC GGGTGAGGTT 
ATGTCCCTGG TGGGGAAAAT GCAGGAGAGC CGCATGGGGG ACCGCTACCA GCGCACCAAA 
CCGCCGCTCA TGGAGGAACG AAAGGCCAAG CGAAAGAAGC GTGACGAGGA CCAGTATGAT 
GTGAAGAAGA TGAAGGGGAT GACGCTGCTG TCGGAGGGGA TTGAGGACAT GGTCGGTATC 
GTGTACCGCC CCAAGACGCA GGAGACGCGA CAAACGTACG AGATGATTCT CAGTGTTATC 
CAGGACGCCC TTGGAGACCA GCCCCGTGAT GTCCTGTGCG GAGCTGCAGA CGAGGTTCTG 
GCCAGTCTGA AGAACGACAG AATGAAAGAG AAGGACAGGC GGAAGGAAGT GGAGCTCCTG 
CTGGGATCCA TGGAGGAGTC ACGTTACGCG CTCATCGTGA ACCTCTGCAA GAAAATCACG 
GATTACGGAG GGGACAAGGA GCAACGGCCG ATTGAAGAGA ACATAGACGA GACCCTTGGT 
GTGAATGTCC AGTTTGAGTC GGATGAGGAG GAGGATGATG ACGATGTGTA CGGCGAGGTT 
CGGGACGCCC AGTCTGATGA TGATGAGGAG GGAGAGGAGG CAGAGATCAC CGGGACACTT 
AAGGCCGAGG GCCTGACGGA GAGTAAAGAC CATGGCAAGG GGAACAAGGA CCTCCACCCT 
CGCGACATCG ATGCCTACTG GCTGCAGCGC CAGCTCGGCA AGATCTACGA CGACCCGATC 
GTCTCGCAGA AACGCGCCGA GGAGATTCTA GAGATCCTGA AGACGGCGTC GGACAACCAC 
GACTGCGAGA ACCAGCTGGT GCGCCTCCTC GGGTTCGACC AGTTCGACTT CATCAAAGTG 
CTGCGGCAGT ATCGCTTCAT GATCCTGTAC TGCACCATGC TGGCTGCGGC ACAGAGCGAG 
GCGGAGCGAC AGCGTATCGT GGATAAGATG GAGAGCGACA CGGAGCTAGC CGCCATCTTG 
CACGCCCTCC AAGAGGAGGA GAAGGGTGAC CTGGTGCAGG AGGAGCGTGC ACGGCGTGCG 
GCGGCCCGTC AGACCCGCGT AGAGGGGGAG CTGGAGGCCA TGCAAGTGGA TGACATCCCA 
GAGGCAATGC GACCAAGTCA GGTGTTAGAC CTGGAGGACC TGATCTTTAC CCAGGGTTCC 
CACCTCATGG CAAATAAACG GTGTCAGCTA CCGGACGGCT CCTACCGCAA ACAGCGCAAA 
GGATATGAAG AGGTCCATGT TCCTGCGCTG AAACCCAAAC CGTTTGCGTC GGACGAGTCT 
CTGGTTTCCA TCGACCGTCT GCCGAAGTAT GCACAGCCGG CTTTCGAGGG CTTCAAGAGT 
CTGAACCGCA TCCAGAGCCG ACTGTACAAG GCCTCGCTGG AGAGTGATGA GAACCTGCTC 
CTGTGTGCTC CCACTGGCGC GGGTAAAACC AACGTGGCCC TGCTGACCAT CCTGCGAGAG 
GTGGGGAAGC ACATCAACCG TGACGGGACC ATCAACGTGG ACGCCTTCAA GATCATCTAC 
ATCGCTCCCA TGAGGTCACT CGTACAGGAG ATGACTGGTA ACTTCAGCAA GAGACTGGAG 
TCTTACGGCA TCACGGTGTC TGAGCTGACT GGGGATCACC AGCTGAGTCG AGAGGAGATC 
CAGGGCTCGC AGGTCATCGT GTGTACGCCG GAGAAGTGGG ACATCATCAC GCGGAAGGCC 
GGGGAGCGGA CGTACACCCA GCTGGTGCGA CTGCTCATCA TTGATGAGAT CCACCTTCTA 
CACGACGAGA GAGGGCCGGT GCTGGAGTGC ATTGTTGCCA GAATGATCCG GAGTATCGAG 
ACCACCCAGG AAGATATCCG ATTGGTCGGC CTGAGCGCCA CCCTGCCGAA CTACGAGGAC 
GTGGCCACGT TCCTGCGTGT GGACCCCGCC AAGGGGCTGT TCTTCTTTGA CAACAGTTTC 
CGCCCTGTGC CGCTGGAACA ACAGTACATC GGTATCACCG AGAAGAAGGC TGTGAAAAGG 
TTCCAGCTGA TGAACGATAT CCTGTATGAG AAGGTGATGG AACACGCGGG GAAGAACCAG 
GTTCTCGTGT TCGTTCACTC GCGGAAGGAG ACGGGAAAGA CGGCACGAGC GATCCGCGAC 
ATGTGTCTGG AAAAGGACAC GCTGGGTCAC TTCCTCCGGG AAGGGTCGGC CGCTACTGAA 
GTGTTGCGTA CAGAGGCAGA GCAGGCAAAG AACCAGGAGC TGAAAGACCT GCTGCCCTAC 
GGTTTCGCCA TCCATCACGC CGGGATGACG CGGGTGGACC GGACGCTGGT GGAGGATCTC 
TTCGCCGATC GCCACATCCA AGTCCTGGTT TCCACGGCAA CCCTCGCCTG GGGTGTCAAC 
CTTCCTGCTC ACACTGTCAT CATCAAGGGC ACACAGGTGT ACAGTCCGGA GAAAGGTCGC 
TGGGTGGAGC TGGGCGCGTT GGACGTCCTG CAGATGTTGG GGCGTGCCGG CCGCCCGCAG 
TACGACACCA AGGGGGAGGG GCTCCTCCTG ACGAGCCACA GTGAACTGCA GTACTACCTG 
TCGCTACAGA ACCAACAGCT GCCTGTGGAA AGTCAGTTCA TCTCCAAGCT GGCAGACAAC 
CTGAATGCTG AGGTAGTGCT GGGTACTGTG CAGAATCTGA AGGATGCTGT CCATTGGCTG 
GGCTACAGCT ACCTGTACAT CCGTATGCTC CGGAATCCCA CGCTGTACGG CGTCTCCTCC 
GACACGAAGG ACGACGCATT GTTGGAGCAG CGCCGTATCG ACCTGATTCA CACGTCGGCC 
GCCACGCTGG ACAAACACAA CCTGATCAAG TACGACAAGA AGACTGGCAA CTTCCAGGTG 
ACAGACTTGG GGCGCATTGC CAGCTACTAC TACCTGACGC ACGACACCAT CGCGACCTAC 
AACCAACTGC TGAAGCCCAC CCTTAGCGAG ATCGAGCTGT TCCGTGTCTT CTCGCTTTCT 
GGCGAGTTCC GAAACATCAC CGTGAGGGAG GAAGAGAAGT TGGAGCTGCA GAAGATGTTG 
GAGAGAGTTC CCATCCCCAT CAAGGAGAGT ATTGAGGAGC CCAGTGCCAA GGTGAATGTT 
CTCTTGCAAG CCTACATCTC CCAGCTGAAG CTGGAAGGTT TCGCGCTGGT GTCTGACATG 
GTGTACGTGA CGCAGTCTGC CGGACGGTTG ATGAGAGCGA TCTTCGAGAT TGTGCTCCAC 
CGTGGCTGGG CGCAGCTGGC AGACAAGGCC CTCGCACTCT GCAAGATGAT TGACAAGAGG 
ATGTGGCAGT CCATGTGCCC GCTCCGACAG TTCCGGAAGA TTCCGGAGGA GGTGGTCAGG 
AAGATTGAGA AGAAGAACTT CCCATGGGAG CGCTTCTACG ACCTGAACCA CAACGAGATT 
GGCGAGCTGA TCCGCATGCC CAAGATGGGG AAGACGCTGC ACAAGTTCAT ACACCAGTTC 
CCTAAGCTGG AGCTGGCCAC ACACATCCAG CCAATCACAA GATCCACCCT GAAGGTGGAA 
CTGACCATCA CACCTGACTT CCAGTGGGAC GATAAGGTCC ACGGGAACTC GGAGGCCTTC 
TGGATCCTGG TGGAGGATGT GGACAGTGAG ATCATCCTGC ACCACGAGTA CTTCCTGCTC 
AAGGCCAAGT TCGCGCAGGA CGAACACATG GTCAAGTTCT TTGTGCCGGT GTTCGAGCCT 
CTCCCTCCAC AGTACTTCAT CAGAGTTGTG TCTGACCGCT GGTTGATGTC GGAGACGCAG 
CTCCCCGTGT CGTTCCGCCA CCTGATCCTC CCGGAGAAGA ACCCGCCCCC CACGGAGCTG 
CTGGACCTGC AGCCCCTGCC CGTGTCCGCT CTCCGGAACT CCCTGTACGA GACTCTGTAC 
CAGAACAAGT TCCCCTGCTT CAACCCAATT CAGACGCAAG TTTTCAACGC GGTGTACAAC 
GGAGATGAGA ACGTGTTCAT CGGGGCGCCG ACAGGAAGCG GGAAGACCAT CTGTGGGGAG 
TTCTCCGTGC TGCGGTTGAT CTCACAGAAC CCAGAGGGGC GCTGTGTCTA CGTGACACCC 
ATGGAGTCAC TTGCCGAGCA GGTGTACAGC GACTGGTACG CCAAGTTCCA GGTACAGCTG 
GGTAAGAAGG TGGTGATGCT GACGGGCGAG ACCAGCACTG ATCTGAAACT CCTGGCCAAG 
GGGAACATCA TCATCAGCAC GCCCGACCGC TGGGACGTGC TCTCACGCCG CTGGAAACAG 
CGCAAGAACG TCCAGAACGT CAACCTGTTC ATCGTGGACG AGGCACATCT GATTGGAGGG 
GAGAATGGGC CCGTGTTGGA GGTCATCTGT TCGCGTATGC GGTACATCGC CTCGCAGATC 
GAGCGTCAGA TCCGAATCGT GGCCCTGAGC TCGTCCCTGT CCAATGCCAA GGACATCGCG 
CAGTGGCTGG GAGCCACCAC CAACTCCACC TTCAACTTCC ACCCCAATGT GCGGCCGGTA 
CCACTGGAGC TGCACATACA GGGCTTCAAC ATCACCCACA CCCCGTCACG ACTGATCGCC 
ATGTCCAAAC CGGCGTACCA CGCCATCCTC AAACATTCCA CCAAGAAGCC CGTCATCATC 
TTTGTGCCGT CACGGAAGCA AGCGCGGATC ACCGCCATCG ATATCCTGAC CTTCGCTGCA 
GCTGACCAGC AACCACAAAG ATTCCTACAC TGTACAGAAG ATGACCTTGG ACCTCACCTG 
AGTAAGGTGT CGGATGCCAC GCTGAAGGAG ACGCTGGTGA ACGGAGTGGC GTACATGCAC 
GAGGGTCTCA CCGACATCGA AAAGAAGATC GTCCAACAGC TGTTTGATGC TGGGGCCATT 
CAGGTCGTGG TGGCATCCCG TAACCTGTGC TGGGCCATCA GCATGCAGGC CCACCTGGTG 
GTCGTCATGG ATACGCAGTT CTACAACGGA AAGATCCACG CCTACGTGGA CTATCCTGTC 
ACGGACGTGC TGCAGATGGT CGGGCGCGCC AACAGGCCTC TCACGGATGA GGATGGAAAG 
TGTGTCATCA TGTGCCCCGC CTCCAAGAAG GACTTCTTTA AGAAGTTCCT GTACGAGCCC 
CTCCCCGTGG AGTCTCACCT GGACCACTGC ATGCACGACC ACTTCAACGC TGAGATCGTG 
ACCAAGACCA TTGAGAACAA GCAGGACGCG GTGGACTACC TGACATGGAC GTTCCTGTAC 
CGCAGGATGA CTCAGAACCC AAACTACTAC AACTTACAGG GTGTGTCTCA CCGCCACTTG 
TCGGACCACC TGTCGGAGGT GGTGGAGCTG ACCTTGAACG ACCTGGAGAA GTCCAAGTGC 
ATCAGCATCG AGGATGAAAT GGATGTGTCG CCGCTCAACC TGGGCATGAT CGCGGCCTAC 
TACTACATCA ACTACACCAC CATTGAATTG TTCAGCATGT CGCTCAACGC CAAGACCAAG 
ATTCGCGGTC TCCTGGAGAT CATCTCCAAT GCTGCGGAGT ACGAGAGCAT CCCCATCCGC 
CACCACGAGG ACTCGGTCCT GCGCCAGCTC TCGGCGCGCC TGCCCAACAA GCTGGCCAAC 
CCCAAGTTCA ACGACCCCCA CATCAAGACC AACCTGCTGC TGCAGGCCCA CCTGTCCCGC 
ATGCAGCTGT CTGCGGAGCT GCAGTCTGAC ACAGAGGAGA TTCTCACTAA GGCTATCCGA 
CTGATCCAAG CGTGCGTGGA CGTCCTGAGC AGTAACGGTT GGCTGAGCCC GGCGCTAGCG 
GCCATGGAGC TGGCCCAGAT GGTGACCCAG GCCATGTGGA GTAAAGACTC CTACCTCAAA 
CAGCTGCCGC ACTTCTCCAA CGACCTCATC AAAAAGTGCA CCGACTCCGG CATCGAGAGT 
GTGTTTGACA TCATGGAGAT GGAAGACGAG GACAGAAACA GTCTCCTGCA GATGAGCGAT 
GCCCAGATGG CGGATGTGGC GCGCTTCTGT AACCGCTACC CCAACATCGA GCTGGCGTAC 
GAAGTCATGG ACAAGGACAA CCTGCACAGT GGCTCCCCTG TGATGATGGT GGTGACACTG 
GAGAGAGAGG ACGAGGCAGC AGGGCCTGTG GTGGCTCCAT TCTTCCCACA GAAACGTGAG 
GAGGGCTGGT GGGTGGTAAT CGGCGACAAC AAGTCCAACA GCCTCATCTC AATCAAGAGG 
CTGACCTTGC AGAACAAGGC CAAGGTCAAA CTGGACTTTG TGGCACCATC TCAAGGGACC 
CACACCTACA CCCTGTACTA CATGAGCGAT GCCTACATGG GCTGTGACCA GGAGTACAAG 
TTCAACATCA ATGTCGGGGA AGAGGCCAGC GATATGGACA GTGACTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002603812.1
CDS	1..6414
Translation	MADASARSLQYEYKANSNLVLQADRSLIDRRSRDEATGEVMSLVGKMQESRMGDRYQRTKPPLMEERKAKRKKRDEDQYDVKKMKGMTLLSEGIEDMVGIVYRPKTQETRQTYEMILSVIQDALGDQPRDVLCGAADEVLASLKNDRMKEKDRRKEVELLLGSMEESRYALIVNLCKKITDYGGDKEQRPIEENIDETLGVNVQFESDEEEDDDDVYGEVRDAQSDDDEEGEEAEITGTLKAEGLTESKDHGKGNKDLHPRDIDAYWLQRQLGKIYDDPIVSQKRAEEILEILKTASDNHDCENQLVRLLGFDQFDFIKVLRQYRFMILYCTMLAAAQSEAERQRIVDKMESDTELAAILHALQEEEKGDLVQEERARRAAARQTRVEGELEAMQVDDIPEAMRPSQVLDLEDLIFTQGSHLMANKRCQLPDGSYRKQRKGYEEVHVPALKPKPFASDESLVSIDRLPKYAQPAFEGFKSLNRIQSRLYKASLESDENLLLCAPTGAGKTNVALLTILREVGKHINRDGTINVDAFKIIYIAPMRSLVQEMTGNFSKRLESYGITVSELTGDHQLSREEIQGSQVIVCTPEKWDIITRKAGERTYTQLVRLLIIDEIHLLHDERGPVLECIVARMIRSIETTQEDIRLVGLSATLPNYEDVATFLRVDPAKGLFFFDNSFRPVPLEQQYIGITEKKAVKRFQLMNDILYEKVMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGHFLREGSAATEVLRTEAEQAKNQELKDLLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWVELGALDVLQMLGRAGRPQYDTKGEGLLLTSHSELQYYLSLQNQQLPVESQFISKLADNLNAEVVLGTVQNLKDAVHWLGYSYLYIRMLRNPTLYGVSSDTKDDALLEQRRIDLIHTSAATLDKHNLIKYDKKTGNFQVTDLGRIASYYYLTHDTIATYNQLLKPTLSEIELFRVFSLSGEFRNITVREEEKLELQKMLERVPIPIKESIEEPSAKVNVLLQAYISQLKLEGFALVSDMVYVTQSAGRLMRAIFEIVLHRGWAQLADKALALCKMIDKRMWQSMCPLRQFRKIPEEVVRKIEKKNFPWERFYDLNHNEIGELIRMPKMGKTLHKFIHQFPKLELATHIQPITRSTLKVELTITPDFQWDDKVHGNSEAFWILVEDVDSEIILHHEYFLLKAKFAQDEHMVKFFVPVFEPLPPQYFIRVVSDRWLMSETQLPVSFRHLILPEKNPPPTELLDLQPLPVSALRNSLYETLYQNKFPCFNPIQTQVFNAVYNGDENVFIGAPTGSGKTICGEFSVLRLISQNPEGRCVYVTPMESLAEQVYSDWYAKFQVQLGKKVVMLTGETSTDLKLLAKGNIIISTPDRWDVLSRRWKQRKNVQNVNLFIVDEAHLIGGENGPVLEVICSRMRYIASQIERQIRIVALSSSLSNAKDIAQWLGATTNSTFNFHPNVRPVPLELHIQGFNITHTPSRLIAMSKPAYHAILKHSTKKPVIIFVPSRKQARITAIDILTFAAADQQPQRFLHCTEDDLGPHLSKVSDATLKETLVNGVAYMHEGLTDIEKKIVQQLFDAGAIQVVVASRNLCWAISMQAHLVVVMDTQFYNGKIHAYVDYPVTDVLQMVGRANRPLTDEDGKCVIMCPASKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGVSHRHLSDHLSEVVELTLNDLEKSKCISIEDEMDVSPLNLGMIAAYYYINYTTIELFSMSLNAKTKIRGLLEIISNAAEYESIPIRHHEDSVLRQLSARLPNKLANPKFNDPHIKTNLLLQAHLSRMQLSAELQSDTEEILTKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFSNDLIKKCTDSGIESVFDIMEMEDEDRNSLLQMSDAQMADVARFCNRYPNIELAYEVMDKDNLHSGSPVMMVVTLEREDEAAGPVVAPFFPQKREEGWWVVIGDNKSNSLISIKRLTLQNKAKVKLDFVAPSQGTHTYTLYYMSDAYMGCDQEYKFNINVGEEASDMDSDSD

Target ORF information:

RefSeq Version	XM_002603766.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002603766.1

ORF Insert Sequence:

ATGGCGGACG CATCGGCTCG TTCGCTTCAG TACGAGTACA AAGCGAACTC CAACCTTGTT 
CTCCAAGCCG ACCGGTCGCT GATTGACCGC AGAAGTCGCG ATGAGGCGAC GGGTGAGGTT 
ATGTCCCTGG TGGGGAAAAT GCAGGAGAGC CGCATGGGGG ACCGCTACCA GCGCACCAAA 
CCGCCGCTCA TGGAGGAACG AAAGGCCAAG CGAAAGAAGC GTGACGAGGA CCAGTATGAT 
GTGAAGAAGA TGAAGGGGAT GACGCTGCTG TCGGAGGGGA TTGAGGACAT GGTCGGTATC 
GTGTACCGCC CCAAGACGCA GGAGACGCGA CAAACGTACG AGATGATTCT CAGTGTTATC 
CAGGACGCCC TTGGAGACCA GCCCCGTGAT GTCCTGTGCG GAGCTGCAGA CGAGGTTCTG 
GCCAGTCTGA AGAACGACAG AATGAAAGAG AAGGACAGGC GGAAGGAAGT GGAGCTCCTG 
CTGGGATCCA TGGAGGAGTC ACGTTACGCG CTCATCGTGA ACCTCTGCAA GAAAATCACG 
GATTACGGAG GGGACAAGGA GCAACGGCCG ATTGAAGAGA ACATAGACGA GACCCTTGGT 
GTGAATGTCC AGTTTGAGTC GGATGAGGAG GAGGATGATG ACGATGTGTA CGGCGAGGTT 
CGGGACGCCC AGTCTGATGA TGATGAGGAG GGAGAGGAGG CAGAGATCAC CGGGACACTT 
AAGGCCGAGG GCCTGACGGA GAGTAAAGAC CATGGCAAGG GGAACAAGGA CCTCCACCCT 
CGCGACATCG ATGCCTACTG GCTGCAGCGC CAGCTCGGCA AGATCTACGA CGACCCGATC 
GTCTCGCAGA AACGCGCCGA GGAGATTCTA GAGATCCTGA AGACGGCGTC GGACAACCAC 
GACTGCGAGA ACCAGCTGGT GCGCCTCCTC GGGTTCGACC AGTTCGACTT CATCAAAGTG 
CTGCGGCAGT ATCGCTTCAT GATCCTGTAC TGCACCATGC TGGCTGCGGC ACAGAGCGAG 
GCGGAGCGAC AGCGTATCGT GGATAAGATG GAGAGCGACA CGGAGCTAGC CGCCATCTTG 
CACGCCCTCC AAGAGGAGGA GAAGGGTGAC CTGGTGCAGG AGGAGCGTGC ACGGCGTGCG 
GCGGCCCGTC AGACCCGCGT AGAGGGGGAG CTGGAGGCCA TGCAAGTGGA TGACATCCCA 
GAGGCAATGC GACCAAGTCA GGTGTTAGAC CTGGAGGACC TGATCTTTAC CCAGGGTTCC 
CACCTCATGG CAAATAAACG GTGTCAGCTA CCGGACGGCT CCTACCGCAA ACAGCGCAAA 
GGATATGAAG AGGTCCATGT TCCTGCGCTG AAACCCAAAC CGTTTGCGTC GGACGAGTCT 
CTGGTTTCCA TCGACCGTCT GCCGAAGTAT GCACAGCCGG CTTTCGAGGG CTTCAAGAGT 
CTGAACCGCA TCCAGAGCCG ACTGTACAAG GCCTCGCTGG AGAGTGATGA GAACCTGCTC 
CTGTGTGCTC CCACTGGCGC GGGTAAAACC AACGTGGCCC TGCTGACCAT CCTGCGAGAG 
GTGGGGAAGC ACATCAACCG TGACGGGACC ATCAACGTGG ACGCCTTCAA GATCATCTAC 
ATCGCTCCCA TGAGGTCACT CGTACAGGAG ATGACTGGTA ACTTCAGCAA GAGACTGGAG 
TCTTACGGCA TCACGGTGTC TGAGCTGACT GGGGATCACC AGCTGAGTCG AGAGGAGATC 
CAGGGCTCGC AGGTCATCGT GTGTACGCCG GAGAAGTGGG ACATCATCAC GCGGAAGGCC 
GGGGAGCGGA CGTACACCCA GCTGGTGCGA CTGCTCATCA TTGATGAGAT CCACCTTCTA 
CACGACGAGA GAGGGCCGGT GCTGGAGTGC ATTGTTGCCA GAATGATCCG GAGTATCGAG 
ACCACCCAGG AAGATATCCG ATTGGTCGGC CTGAGCGCCA CCCTGCCGAA CTACGAGGAC 
GTGGCCACGT TCCTGCGTGT GGACCCCGCC AAGGGGCTGT TCTTCTTTGA CAACAGTTTC 
CGCCCTGTGC CGCTGGAACA ACAGTACATC GGTATCACCG AGAAGAAGGC TGTGAAAAGG 
TTCCAGCTGA TGAACGATAT CCTGTATGAG AAGGTGATGG AACACGCGGG GAAGAACCAG 
GTTCTCGTGT TCGTTCACTC GCGGAAGGAG ACGGGAAAGA CGGCACGAGC GATCCGCGAC 
ATGTGTCTGG AAAAGGACAC GCTGGGTCAC TTCCTCCGGG AAGGGTCGGC CGCTACTGAA 
GTGTTGCGTA CAGAGGCAGA GCAGGCAAAG AACCAGGAGC TGAAAGACCT GCTGCCCTAC 
GGTTTCGCCA TCCATCACGC CGGGATGACG CGGGTGGACC GGACGCTGGT GGAGGATCTC 
TTCGCCGATC GCCACATCCA AGTCCTGGTT TCCACGGCAA CCCTCGCCTG GGGTGTCAAC 
CTTCCTGCTC ACACTGTCAT CATCAAGGGC ACACAGGTGT ACAGTCCGGA GAAAGGTCGC 
TGGGTGGAGC TGGGCGCGTT GGACGTCCTG CAGATGTTGG GGCGTGCCGG CCGCCCGCAG 
TACGACACCA AGGGGGAGGG GCTCCTCCTG ACGAGCCACA GTGAACTGCA GTACTACCTG 
TCGCTACAGA ACCAACAGCT GCCTGTGGAA AGTCAGTTCA TCTCCAAGCT GGCAGACAAC 
CTGAATGCTG AGGTAGTGCT GGGTACTGTG CAGAATCTGA AGGATGCTGT CCATTGGCTG 
GGCTACAGCT ACCTGTACAT CCGTATGCTC CGGAATCCCA CGCTGTACGG CGTCTCCTCC 
GACACGAAGG ACGACGCATT GTTGGAGCAG CGCCGTATCG ACCTGATTCA CACGTCGGCC 
GCCACGCTGG ACAAACACAA CCTGATCAAG TACGACAAGA AGACTGGCAA CTTCCAGGTG 
ACAGACTTGG GGCGCATTGC CAGCTACTAC TACCTGACGC ACGACACCAT CGCGACCTAC 
AACCAACTGC TGAAGCCCAC CCTTAGCGAG ATCGAGCTGT TCCGTGTCTT CTCGCTTTCT 
GGCGAGTTCC GAAACATCAC CGTGAGGGAG GAAGAGAAGT TGGAGCTGCA GAAGATGTTG 
GAGAGAGTTC CCATCCCCAT CAAGGAGAGT ATTGAGGAGC CCAGTGCCAA GGTGAATGTT 
CTCTTGCAAG CCTACATCTC CCAGCTGAAG CTGGAAGGTT TCGCGCTGGT GTCTGACATG 
GTGTACGTGA CGCAGTCTGC CGGACGGTTG ATGAGAGCGA TCTTCGAGAT TGTGCTCCAC 
CGTGGCTGGG CGCAGCTGGC AGACAAGGCC CTCGCACTCT GCAAGATGAT TGACAAGAGG 
ATGTGGCAGT CCATGTGCCC GCTCCGACAG TTCCGGAAGA TTCCGGAGGA GGTGGTCAGG 
AAGATTGAGA AGAAGAACTT CCCATGGGAG CGCTTCTACG ACCTGAACCA CAACGAGATT 
GGCGAGCTGA TCCGCATGCC CAAGATGGGG AAGACGCTGC ACAAGTTCAT ACACCAGTTC 
CCTAAGCTGG AGCTGGCCAC ACACATCCAG CCAATCACAA GATCCACCCT GAAGGTGGAA 
CTGACCATCA CACCTGACTT CCAGTGGGAC GATAAGGTCC ACGGGAACTC GGAGGCCTTC 
TGGATCCTGG TGGAGGATGT GGACAGTGAG ATCATCCTGC ACCACGAGTA CTTCCTGCTC 
AAGGCCAAGT TCGCGCAGGA CGAACACATG GTCAAGTTCT TTGTGCCGGT GTTCGAGCCT 
CTCCCTCCAC AGTACTTCAT CAGAGTTGTG TCTGACCGCT GGTTGATGTC GGAGACGCAG 
CTCCCCGTGT CGTTCCGCCA CCTGATCCTC CCGGAGAAGA ACCCGCCCCC CACGGAGCTG 
CTGGACCTGC AGCCCCTGCC CGTGTCCGCT CTCCGGAACT CCCTGTACGA GACTCTGTAC 
CAGAACAAGT TCCCCTGCTT CAACCCAATT CAGACGCAAG TTTTCAACGC GGTGTACAAC 
GGAGATGAGA ACGTGTTCAT CGGGGCGCCG ACAGGAAGCG GGAAGACCAT CTGTGGGGAG 
TTCTCCGTGC TGCGGTTGAT CTCACAGAAC CCAGAGGGGC GCTGTGTCTA CGTGACACCC 
ATGGAGTCAC TTGCCGAGCA GGTGTACAGC GACTGGTACG CCAAGTTCCA GGTACAGCTG 
GGTAAGAAGG TGGTGATGCT GACGGGCGAG ACCAGCACTG ATCTGAAACT CCTGGCCAAG 
GGGAACATCA TCATCAGCAC GCCCGACCGC TGGGACGTGC TCTCACGCCG CTGGAAACAG 
CGCAAGAACG TCCAGAACGT CAACCTGTTC ATCGTGGACG AGGCACATCT GATTGGAGGG 
GAGAATGGGC CCGTGTTGGA GGTCATCTGT TCGCGTATGC GGTACATCGC CTCGCAGATC 
GAGCGTCAGA TCCGAATCGT GGCCCTGAGC TCGTCCCTGT CCAATGCCAA GGACATCGCG 
CAGTGGCTGG GAGCCACCAC CAACTCCACC TTCAACTTCC ACCCCAATGT GCGGCCGGTA 
CCACTGGAGC TGCACATACA GGGCTTCAAC ATCACCCACA CCCCGTCACG ACTGATCGCC 
ATGTCCAAAC CGGCGTACCA CGCCATCCTC AAACATTCCA CCAAGAAGCC CGTCATCATC 
TTTGTGCCGT CACGGAAGCA AGCGCGGATC ACCGCCATCG ATATCCTGAC CTTCGCTGCA 
GCTGACCAGC AACCACAAAG ATTCCTACAC TGTACAGAAG ATGACCTTGG ACCTCACCTG 
AGTAAGGTGT CGGATGCCAC GCTGAAGGAG ACGCTGGTGA ACGGAGTGGC GTACATGCAC 
GAGGGTCTCA CCGACATCGA AAAGAAGATC GTCCAACAGC TGTTTGATGC TGGGGCCATT 
CAGGTCGTGG TGGCATCCCG TAACCTGTGC TGGGCCATCA GCATGCAGGC CCACCTGGTG 
GTCGTCATGG ATACGCAGTT CTACAACGGA AAGATCCACG CCTACGTGGA CTATCCTGTC 
ACGGACGTGC TGCAGATGGT CGGGCGCGCC AACAGGCCTC TCACGGATGA GGATGGAAAG 
TGTGTCATCA TGTGCCCCGC CTCCAAGAAG GACTTCTTTA AGAAGTTCCT GTACGAGCCC 
CTCCCCGTGG AGTCTCACCT GGACCACTGC ATGCACGACC ACTTCAACGC TGAGATCGTG 
ACCAAGACCA TTGAGAACAA GCAGGACGCG GTGGACTACC TGACATGGAC GTTCCTGTAC 
CGCAGGATGA CTCAGAACCC AAACTACTAC AACTTACAGG GTGTGTCTCA CCGCCACTTG 
TCGGACCACC TGTCGGAGGT GGTGGAGCTG ACCTTGAACG ACCTGGAGAA GTCCAAGTGC 
ATCAGCATCG AGGATGAAAT GGATGTGTCG CCGCTCAACC TGGGCATGAT CGCGGCCTAC 
TACTACATCA ACTACACCAC CATTGAATTG TTCAGCATGT CGCTCAACGC CAAGACCAAG 
ATTCGCGGTC TCCTGGAGAT CATCTCCAAT GCTGCGGAGT ACGAGAGCAT CCCCATCCGC 
CACCACGAGG ACTCGGTCCT GCGCCAGCTC TCGGCGCGCC TGCCCAACAA GCTGGCCAAC 
CCCAAGTTCA ACGACCCCCA CATCAAGACC AACCTGCTGC TGCAGGCCCA CCTGTCCCGC 
ATGCAGCTGT CTGCGGAGCT GCAGTCTGAC ACAGAGGAGA TTCTCACTAA GGCTATCCGA 
CTGATCCAAG CGTGCGTGGA CGTCCTGAGC AGTAACGGTT GGCTGAGCCC GGCGCTAGCG 
GCCATGGAGC TGGCCCAGAT GGTGACCCAG GCCATGTGGA GTAAAGACTC CTACCTCAAA 
CAGCTGCCGC ACTTCTCCAA CGACCTCATC AAAAAGTGCA CCGACTCCGG CATCGAGAGT 
GTGTTTGACA TCATGGAGAT GGAAGACGAG GACAGAAACA GTCTCCTGCA GATGAGCGAT 
GCCCAGATGG CGGATGTGGC GCGCTTCTGT AACCGCTACC CCAACATCGA GCTGGCGTAC 
GAAGTCATGG ACAAGGACAA CCTGCACAGT GGCTCCCCTG TGATGATGGT GGTGACACTG 
GAGAGAGAGG ACGAGGCAGC AGGGCCTGTG GTGGCTCCAT TCTTCCCACA GAAACGTGAG 
GAGGGCTGGT GGGTGGTAAT CGGCGACAAC AAGTCCAACA GCCTCATCTC AATCAAGAGG 
CTGACCTTGC AGAACAAGGC CAAGGTCAAA CTGGACTTTG TGGCACCATC TCAAGGGACC 
CACACCTACA CCCTGTACTA CATGAGCGAT GCCTACATGG GCTGTGACCA GGAGTACAAG 
TTCAACATCA ATGTCGGGGA AGAGGCCAGC GATATGGACA GTGACTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_124682 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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