BRAFLDRAFT_83576 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_83576
Entrez Gene ID	7239721
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002591742.1	XP_002591788.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_83576 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_83576 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb14636	XM_002591742.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_83576 ( XM_002591742.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002591788.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb14636
Clone ID Related Accession (Same CDS sequence)	XM_002591742.1
Accession Version	XM_002591742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101395). COMPLETENESS: incomplete on both ends.

ATGGCGGGAC AACACGGATT TGTCCTGCTG ACCGCCGCGC TCTTCCTACA GGCCTGTCGG 
GGGCAGGTGC TGACTCCCCC GTACCTAAAC CTGGCACAAG GCCGGCGGAT CGTCGCATCG 
GCCACTTGTG GTGAAGGCGT TCCCGAGCCG GAGCTGTACT GTAAGCTGGC CGGCGGGACC 
GGCAACGAGC CGGCTAACCT GATCCAAGGC CAGCAGTGCG ACTACTGCGA CCCCCGGGAC 
CCCTCGCGCG CTCATCCCGT CTCGCAGGCC GTGGACGGCA CCGAGAAATG GTGGCAGAGC 
CCTCCCCTAT CCCGTGGAAC CGACTACAAC AGAGTCAACG TCACCATCAG CCTTGGACAG 
TTGTTCCATG TGGCTTACGT GGTACTGAAG TTCGCCAATG CCCCCCGCCC GGGTGTCTGG 
GTTCTGGAGC GGTCCACGGA CTTCGGGCAG ACCTACAAGC CATGGCAGTA CTTTGCCGAC 
ACGCCGAGCG ACTGCTACAA CGTCTTCGGC GTCACCCCCA TGGACAGACC GACACATGAC 
GACTCCGTCA CCTGCACCAC GGAGTACTCC AACATCGTAC CTCTGGAAAA CGGCGAGATC 
CACCTGTCGC TGGTGAATGG CCGTCCCAGT TCCAAGAACT TCACCCATTC CTCCATGCTG 
CACGAGTGGA CCAAGGCCAC CAATGTGCGA CTCAGGTTCC TCCGGACTAA GACACTGCTG 
GGTCATCTCA TGGCGGTCAC ACAGCAGGAC CCCACTGTCA CCAGAAGGTA TTTCTACAGT 
TTGAAGGACA TCTCTATCGG CGGACGGTGT GTGTGTAACG GGCATGCGGA CGAGTGCGAT 
GGAACAGGGT CTGCAGATCC CAACAGACTT GTGTGTAAGT GCCAGCACAA CACCTGTGGG 
GAGTCATGTG AGCAGTGCTG TCCTGGATTT GTGCAGAAAC CATGGCAACC GGCCACACCT 
AACAACCTGT TTGAATGTGA ATCCTGTAAC TGCCTGGGCC ACTCTACTGA GTGTTACTAT 
GACCCTGAGG TGGCACAGAA CCGACTCAGT CTGGACATGA GGGGAGACTT CAATGGAGGG 
GGTGTCTGCA TCAACTGTCA GCACAACACT GAAGGCATCA ACTGTGAGCG GTGCAAACCC 
GGGTTCTTCC GAGTGCCTGA CACCCCTCTA ACATCACCCT ATGTCTGCCA ATCCTGCCAG 
TGTAACTCTT TCTTCACCAC TGGGACATGT GAACAGCTGA CTGGACGTTG TTACTGTCTG 
CCTGGCTATG CTGGTGATGA CTGTAACCAG TGTGCGTCTG GTTATGTGGG CTTCCCAAAC 
TGCAAAGCCT TGTTGGTCAA AAAGACTAAG AGCAGACCCA ACCCAGAAGC AGAGGTGTTC 
ATTCCCCTGA CTTCTGAGAT TGTGGACATC CCTCCTCCAT ATAGTCAACC AAAAGTGGTC 
CAGCCAACTC CAGGATATGA TGGAGGCTAC AGTGCCTATG GCCTCTTGTG TGAGTGCCAC 
ATGAATGGTA CAGCAGGCTT CACCTGTTCA GTGGAGGGTG ATGGCCAGTG TCCCTGTAAG 
TACAACTATG CCGGGCGCTA CTGCGAGGGC TGTGCACTTG GGTACTACAA CTTCCCAGAG 
TGTCTACCCT GTCAGTGTGA TGAAGCCGGT TCTCTGTCCC ACCGGTGTGA CATCCAGCAG 
GGCCAGTGTG AATGTCGCAG TGGCTTCATG GGCCGGACAT GTGAGCGCTG TGCCACTGGG 
TACTACAACT ACCCCAGATG CACCTCCTGT GACTGTAATG TGATGGGAGC TACCCAGGAG 
GTATGTGATG CTGATACTGG CACCTGTCTG TGTCGGGAAC CTTTCACCGG TCCGAGGTGC 
GACCAGTGCA AGTCTGGGTC CTACAGTTTC CCAAACTGCA TACGTTGTAA CTGCTATGGT 
GCAGGTGTGC TAGATGATGA CACCTGCTCC TGTGAGGCGG GTGGGTCCAT AGGCAGTGAC 
TGTGACCCCA GGTCAGGACA GTGCCGCTGT AAGGAACATG TGGAGGGGAG AAACTGCAAC 
AAGTGTCAAG TCGGCTTCTT CAACTTCCCT AACTGCCTAG AGTGCAACTG TAACCCAGCA 
GGCCGGGCCC CTGACACTGA GTGTCCGTCA GTCCAAACAA TGACGTGTGA CTGTAAGAGC 
CTTGTGGAAG GCCCAGCCTG TGACCAGTGT AAGCCCCTGT ACTGGAACCT GCTGAAGGAC 
AGACCTGAGG GGTGTGAGGA GTGTGGATGC TTCAGACCTG GGACCAGCAG TGGACTGGGC 
ACTTGTGATA TGGCCATAGG TCAGTGTGCC TGCAAAGCTA ACGTCCAGGG GAGGATCTGT 
ATGGACTGTA GGGATGGAAG CTTTGGGATG TCGGAGGTGG ACAGTCTCGG ATGTCAGGGT 
TGCAGTTGTG ACGTAGGTGG GGCAGTGACA GATGTGTGTG ACAAGGCCAC AGGACAGTGC 
GTGTGCAGGG AGGGGATGGA GGGGCGGCAG TGTGACGTCC CCACCCAGGG GCAGTACGTC 
CCGGGGCTGT ACCAGTACAG GTACGAGGCC GAGGATGCAG ACACACCCCG CGGCATGCCC 
GTCAGGTTTG GGTACTCTCC AAAGGATTTC CCAGGGTTCA GCTGGAGGGG ATATGCAATC 
ATGAATACCG TCCAGCCAAG GATAATCCTG TCAGTCCTAG TTGACCGCCC TGCGCTGTAC 
CGTGTGGTTC TGTCATACGT CAACCCGGGA CAGGAGACTG TCATTGGCAA CATTCGAGCA 
AACCCGCTGT CTCAGTCAGC TGATGTTGTT TCCCAGCAGA GCAGTGAGAT AGTGTTTAAC 
CCCACCACCA CCCCGACAAC TGTCACTGTG GTGGGGAACA GTTTCATCAT GCCCTTCGTG 
CTCAACCCAG GACAATGGAG CTTCACCATT GAGGTGTCAA GGGTCCTGTT GGACTACATG 
GTGCTGCTGC CTGCAGCCTA CTTTGAGGCC CCACTGCTGC AGCTGAAGGT GGAGGTCCCC 
TGCAAGTATG AGATTCCAGA GGACCCCAAA GACCACTGCC TGATGTACAC CCATCTGCCC 
CTGGAAGACT TCACCACAGT CCAGGCAGAG TCTGGGTTCT TCCGCCCGCA GCAGGGCGAT 
GATGACATGA ACAACACCCT GTCTGTCCTG GGAGACAAGA TCGCTACACA GTACATCCAG 
CCCTCCAAGT TCTACCCACT CATGGCACTG TTGGATCACA ATCAGATGGT TCTACTTCTG 
GAGCTGACCC TGCCCAAGGC TGGCGAGTAT GTGTTTGTGA TCGAGTATGC CAACTTTGAG 
ACACGGGCAC AGACCCTGCA GATCCGTGTG GACAAGACAG AGGGCAAGAA TCAAGGCATC 
TTCAACATCA ACACCTGCAA CTTTAGTTTC CGCTGTCGTG CTGTTGGGAT TGACGACAAC 
GGGAGAGAGG CACGCTTCAA GGTTACCTCA AAGTCAACCA CCATCAGTCT GTCTGGAGAT 
AAGCTCAGAG TTGCCATTGA CTCACTGACT GCTATCCCTG TGGAATCATG GGACCCAGAT 
TACATCATTC CCAAACCTCA GTGTGTGGCC AGTTACGGGC CATTCTCCAA TCACAAGTGT 
GTCCAGTCCA TGTACACAGA ACCAGTCGGA ACAGTAAGGA TCATGCCGAG TTCTGGACAG 
GACTCGATGC GTCGCCAGCC TCTCCTGGTC CTGTCCAACT CCAAACTCTT CCTGCTGGAC 
AGGGAACAGA ACCAACTTGA GTTTAGTGGC CGTCTCCTCT CAGCTGGTAG AATGGTGTTC 
CTGCTCCACT ACTTCCAGCC TGAGAGTACA GGGTACACTG CACAGGTCAA GGTCACAACA 
CCGCTCAGGC AGGAGATTCA AGGAGTTATC CAGGTTGACT ACTGTCCCCA CAGTGCCGGC 
TGTCGTGCCA TCATGCTTTT CTCAGGTCTG GGACAGGAGG TGGACATCAC CCAGCCTGAC 
ATCAGGCTCA TGATCACTGT TCCTGCAGAC AGAACCATCT GGGTGGACCA TGTTGTGGCT 
ATTCCAGAGT TCAGCTACAC CCCCTCCCTT CTGATGGACA GCAGGATTGA CAAGTCTGGT 
GACTTCATCA AGCAGTGTGG CAAATTTGAC TTCAACCTCA ATGACAGGGA CCTGACTCCC 
TTCTGCCGGA AGTCCATCAT GTCACTGTCT GCCCACTACA ACGATGGGGC AACCCCCTGC 
AACTGTAATC CCCGGGGCTC TTCCAGCCAG ACCTGCTCCC CCCTGGGTGG ACAGTGTCCC 
TGTAAGAACA ACATCGTGGG GCGCAGCTGT GATGTCTGTG CTACTGGGTA CTTTGGCTTC 
CCTGACTGCA GAGCCTGCCC ATGTGGAGAG AACCGCCTGT GTGACCCGGT GACGGGAGAG 
TGCATCTGTC CGCCACGTTC CCGCCGGCCT GAGTGTACCG TCTGTGAGGA GGGGACGTAC 
GGGTACCACC CACTGCTGGG ATGTGAGCCA TGTGACTGTG ATGCCATGGG TATCCTGGAC 
CTGAACTACC CATCATGTAA CAGGAACACA GGGCAGTGCA GCTGCAAGCC AAACGTAGGA 
GGCCGACGTT GTGACCGCTG CCTGCCAGGC TACTACAACT TCCCCTACTG CTCCCCCTGT 
AGCTGTAGTG CTGATGGGGT CACTGAGGAT GTGTGTGACC AGAGAACAGG AAGGTGTCTG 
TGTAAGACCA ATGTGGATGG CGTGAGGTGT GACCAGTGTA AGGCCAACAC CTACCACCTG 
TCAGCCAGGA ACCCTGAGGG ATGCCACAGC TGCTTCTGCT TCGGTGCCAC ACCTTACTGT 
AGGAGCAGTG ACTTGCATTA CACAGAGCTT TTGGACATGG AGGACTGGAC CGTGTCCAAC 
CAGGTTGGGT CAGAGGTCAT GCGGTTTAAC GACAAGGTGG AGGTGAAGCT GGGAGAACTG 
ATCCAACCAG ACGAGCCCGT GTACTGGGTG GCACCAGGCA TCTACCTCGG CAACTTGGTA 
ACATCATATG GTGGGAGCCT CACCTATACT GTAGAGTACC AGGTGACTAA GACCGCCCTG 
ATGGCCAGGA TGCGCCCCAC CAGGCTGCCT GATGTGCTCC TATCTGGTAA TGACATGACC 
ATCATGTACT CCCACCCTGT CATCCCTGAG CCTGATGAGC CCCTCACTGT TACTGTGGAC 
CTGCTGGAGG GTAACTTTGT TCACAGTAAC AGTAAGGGTG CCGTGAGCCG GGAGGATCTG 
CTGATGGTAC TGGCTGATCT CCAGGCACTG CTCATCAGGG CTTCCTACAG GGAGAAGATT 
CTCAGTGCTA CTATTGAGTC AGTAAAGCTG AGCATCACGA CCCAAACCTT CTCTGGCAAG 
CCTGCCTTGG ATGTGGAGTA CTGTAACTGT CCATATGGAT ACAAAGGGAC TTCATGTCAG 
GAGTGTGATG AGGGTTACTA CAGGTCTAAG ACTGGTCCGT ACCTGGGCCA GTGTGTGAAG 
TGTGAGTGTT ACGGGCATGC CAGCAGCTGT GATGAGAATG GAGCATGCAT TGACTGTCAG 
CATCACACTA TGGGAGAGAG GTGTGACAAG TGCATGACTG GTTACTATGG TGAGCCCAGG 
AGGGGCACAC CCGACGACTG TCAGATCTGT GCCTGCCCCA TGGCAACAGA AGACAACAAC 
TTTGCCTCAG AGTGTCAGCC ACTTGCAGGT GGACAGTTCC AGTGCACCTG TCTGCCAGGA 
TATGATGGAC CAAACTGTGA CAGATGTGCT ATTGGCTTCT ATGGGAAGCC CAGTGAGGTG 
GGCGGGTTCT GTCAGCGCTG TGACTGCAGT GGGAATGTCC GGCTGACCAA GGTGGGGATG 
AAGAATGTGG AACAGTGTGA CGCAGAGACA GGACAGTGTG TGGACTGTCT GGACAACACC 
GGGGGCCGCA ACTGTCAGAA GTGTGCTGCT GGGTTCTATG GAGATGCCAT CAAGACCAGG 
AGTTGTTCAA AATGCAGGAA CTGTGATGTT TGCGGCACAG TACCTGATGT GTGCGATGAC 
AAGACGGGCA AGTGTCTGTG TCGGGACCAT GTGGTAGGGG ACAACTGTGA TCAGTGTGAG 
GAGAACCACT ATGGGTTTGA CTCGTGCCAA GGCTGCCAGG CCTGTGACTG TTCAGCGGAC 
GGTTCCTTGG CCAAGTCCTG TCACCCCAAG ACTGGTCAGT GCACCTGTAA GCCGGGTGTG 
ACCGGCCGGC GCTGTGACCG GTGTGAGGCC AAACACTCCA ACTTCACCAG TAGTGGATGC 
AGAGCTTGCA ACTGTCCAAA GGATGGACCA TGCGACCCAC AGACGGGGGA GTGCAGGTGT 
CCTGAGGGAC TGACAGGGAC AGACTGTGAG AGCTGCAAAA ATGAAACCCT CCTGTACACC 
CCCAGAGGGT GCTGGGGTGA GTTGGTCCAC TTGAATGTGT CAGACTGCAA TATGTTCACA 
TACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002591788.1
CDS	1..6429
Translation	MAGQHGFVLLTAALFLQACRGQVLTPPYLNLAQGRRIVASATCGEGVPEPELYCKLAGGTGNEPANLIQGQQCDYCDPRDPSRAHPVSQAVDGTEKWWQSPPLSRGTDYNRVNVTISLGQLFHVAYVVLKFANAPRPGVWVLERSTDFGQTYKPWQYFADTPSDCYNVFGVTPMDRPTHDDSVTCTTEYSNIVPLENGEIHLSLVNGRPSSKNFTHSSMLHEWTKATNVRLRFLRTKTLLGHLMAVTQQDPTVTRRYFYSLKDISIGGRCVCNGHADECDGTGSADPNRLVCKCQHNTCGESCEQCCPGFVQKPWQPATPNNLFECESCNCLGHSTECYYDPEVAQNRLSLDMRGDFNGGGVCINCQHNTEGINCERCKPGFFRVPDTPLTSPYVCQSCQCNSFFTTGTCEQLTGRCYCLPGYAGDDCNQCASGYVGFPNCKALLVKKTKSRPNPEAEVFIPLTSEIVDIPPPYSQPKVVQPTPGYDGGYSAYGLLCECHMNGTAGFTCSVEGDGQCPCKYNYAGRYCEGCALGYYNFPECLPCQCDEAGSLSHRCDIQQGQCECRSGFMGRTCERCATGYYNYPRCTSCDCNVMGATQEVCDADTGTCLCREPFTGPRCDQCKSGSYSFPNCIRCNCYGAGVLDDDTCSCEAGGSIGSDCDPRSGQCRCKEHVEGRNCNKCQVGFFNFPNCLECNCNPAGRAPDTECPSVQTMTCDCKSLVEGPACDQCKPLYWNLLKDRPEGCEECGCFRPGTSSGLGTCDMAIGQCACKANVQGRICMDCRDGSFGMSEVDSLGCQGCSCDVGGAVTDVCDKATGQCVCREGMEGRQCDVPTQGQYVPGLYQYRYEAEDADTPRGMPVRFGYSPKDFPGFSWRGYAIMNTVQPRIILSVLVDRPALYRVVLSYVNPGQETVIGNIRANPLSQSADVVSQQSSEIVFNPTTTPTTVTVVGNSFIMPFVLNPGQWSFTIEVSRVLLDYMVLLPAAYFEAPLLQLKVEVPCKYEIPEDPKDHCLMYTHLPLEDFTTVQAESGFFRPQQGDDDMNNTLSVLGDKIATQYIQPSKFYPLMALLDHNQMVLLLELTLPKAGEYVFVIEYANFETRAQTLQIRVDKTEGKNQGIFNINTCNFSFRCRAVGIDDNGREARFKVTSKSTTISLSGDKLRVAIDSLTAIPVESWDPDYIIPKPQCVASYGPFSNHKCVQSMYTEPVGTVRIMPSSGQDSMRRQPLLVLSNSKLFLLDREQNQLEFSGRLLSAGRMVFLLHYFQPESTGYTAQVKVTTPLRQEIQGVIQVDYCPHSAGCRAIMLFSGLGQEVDITQPDIRLMITVPADRTIWVDHVVAIPEFSYTPSLLMDSRIDKSGDFIKQCGKFDFNLNDRDLTPFCRKSIMSLSAHYNDGATPCNCNPRGSSSQTCSPLGGQCPCKNNIVGRSCDVCATGYFGFPDCRACPCGENRLCDPVTGECICPPRSRRPECTVCEEGTYGYHPLLGCEPCDCDAMGILDLNYPSCNRNTGQCSCKPNVGGRRCDRCLPGYYNFPYCSPCSCSADGVTEDVCDQRTGRCLCKTNVDGVRCDQCKANTYHLSARNPEGCHSCFCFGATPYCRSSDLHYTELLDMEDWTVSNQVGSEVMRFNDKVEVKLGELIQPDEPVYWVAPGIYLGNLVTSYGGSLTYTVEYQVTKTALMARMRPTRLPDVLLSGNDMTIMYSHPVIPEPDEPLTVTVDLLEGNFVHSNSKGAVSREDLLMVLADLQALLIRASYREKILSATIESVKLSITTQTFSGKPALDVEYCNCPYGYKGTSCQECDEGYYRSKTGPYLGQCVKCECYGHASSCDENGACIDCQHHTMGERCDKCMTGYYGEPRRGTPDDCQICACPMATEDNNFASECQPLAGGQFQCTCLPGYDGPNCDRCAIGFYGKPSEVGGFCQRCDCSGNVRLTKVGMKNVEQCDAETGQCVDCLDNTGGRNCQKCAAGFYGDAIKTRSCSKCRNCDVCGTVPDVCDDKTGKCLCRDHVVGDNCDQCEENHYGFDSCQGCQACDCSADGSLAKSCHPKTGQCTCKPGVTGRRCDRCEAKHSNFTSSGCRACNCPKDGPCDPQTGECRCPEGLTGTDCESCKNETLLYTPRGCWGELVHLNVSDCNMFTYD

Target ORF information:

RefSeq Version	XM_002591742.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002591742.1

ORF Insert Sequence:

ATGGCGGGAC AACACGGATT TGTCCTGCTG ACCGCCGCGC TCTTCCTACA GGCCTGTCGG 
GGGCAGGTGC TGACTCCCCC GTACCTAAAC CTGGCACAAG GCCGGCGGAT CGTCGCATCG 
GCCACTTGTG GTGAAGGCGT TCCCGAGCCG GAGCTGTACT GTAAGCTGGC CGGCGGGACC 
GGCAACGAGC CGGCTAACCT GATCCAAGGC CAGCAGTGCG ACTACTGCGA CCCCCGGGAC 
CCCTCGCGCG CTCATCCCGT CTCGCAGGCC GTGGACGGCA CCGAGAAATG GTGGCAGAGC 
CCTCCCCTAT CCCGTGGAAC CGACTACAAC AGAGTCAACG TCACCATCAG CCTTGGACAG 
TTGTTCCATG TGGCTTACGT GGTACTGAAG TTCGCCAATG CCCCCCGCCC GGGTGTCTGG 
GTTCTGGAGC GGTCCACGGA CTTCGGGCAG ACCTACAAGC CATGGCAGTA CTTTGCCGAC 
ACGCCGAGCG ACTGCTACAA CGTCTTCGGC GTCACCCCCA TGGACAGACC GACACATGAC 
GACTCCGTCA CCTGCACCAC GGAGTACTCC AACATCGTAC CTCTGGAAAA CGGCGAGATC 
CACCTGTCGC TGGTGAATGG CCGTCCCAGT TCCAAGAACT TCACCCATTC CTCCATGCTG 
CACGAGTGGA CCAAGGCCAC CAATGTGCGA CTCAGGTTCC TCCGGACTAA GACACTGCTG 
GGTCATCTCA TGGCGGTCAC ACAGCAGGAC CCCACTGTCA CCAGAAGGTA TTTCTACAGT 
TTGAAGGACA TCTCTATCGG CGGACGGTGT GTGTGTAACG GGCATGCGGA CGAGTGCGAT 
GGAACAGGGT CTGCAGATCC CAACAGACTT GTGTGTAAGT GCCAGCACAA CACCTGTGGG 
GAGTCATGTG AGCAGTGCTG TCCTGGATTT GTGCAGAAAC CATGGCAACC GGCCACACCT 
AACAACCTGT TTGAATGTGA ATCCTGTAAC TGCCTGGGCC ACTCTACTGA GTGTTACTAT 
GACCCTGAGG TGGCACAGAA CCGACTCAGT CTGGACATGA GGGGAGACTT CAATGGAGGG 
GGTGTCTGCA TCAACTGTCA GCACAACACT GAAGGCATCA ACTGTGAGCG GTGCAAACCC 
GGGTTCTTCC GAGTGCCTGA CACCCCTCTA ACATCACCCT ATGTCTGCCA ATCCTGCCAG 
TGTAACTCTT TCTTCACCAC TGGGACATGT GAACAGCTGA CTGGACGTTG TTACTGTCTG 
CCTGGCTATG CTGGTGATGA CTGTAACCAG TGTGCGTCTG GTTATGTGGG CTTCCCAAAC 
TGCAAAGCCT TGTTGGTCAA AAAGACTAAG AGCAGACCCA ACCCAGAAGC AGAGGTGTTC 
ATTCCCCTGA CTTCTGAGAT TGTGGACATC CCTCCTCCAT ATAGTCAACC AAAAGTGGTC 
CAGCCAACTC CAGGATATGA TGGAGGCTAC AGTGCCTATG GCCTCTTGTG TGAGTGCCAC 
ATGAATGGTA CAGCAGGCTT CACCTGTTCA GTGGAGGGTG ATGGCCAGTG TCCCTGTAAG 
TACAACTATG CCGGGCGCTA CTGCGAGGGC TGTGCACTTG GGTACTACAA CTTCCCAGAG 
TGTCTACCCT GTCAGTGTGA TGAAGCCGGT TCTCTGTCCC ACCGGTGTGA CATCCAGCAG 
GGCCAGTGTG AATGTCGCAG TGGCTTCATG GGCCGGACAT GTGAGCGCTG TGCCACTGGG 
TACTACAACT ACCCCAGATG CACCTCCTGT GACTGTAATG TGATGGGAGC TACCCAGGAG 
GTATGTGATG CTGATACTGG CACCTGTCTG TGTCGGGAAC CTTTCACCGG TCCGAGGTGC 
GACCAGTGCA AGTCTGGGTC CTACAGTTTC CCAAACTGCA TACGTTGTAA CTGCTATGGT 
GCAGGTGTGC TAGATGATGA CACCTGCTCC TGTGAGGCGG GTGGGTCCAT AGGCAGTGAC 
TGTGACCCCA GGTCAGGACA GTGCCGCTGT AAGGAACATG TGGAGGGGAG AAACTGCAAC 
AAGTGTCAAG TCGGCTTCTT CAACTTCCCT AACTGCCTAG AGTGCAACTG TAACCCAGCA 
GGCCGGGCCC CTGACACTGA GTGTCCGTCA GTCCAAACAA TGACGTGTGA CTGTAAGAGC 
CTTGTGGAAG GCCCAGCCTG TGACCAGTGT AAGCCCCTGT ACTGGAACCT GCTGAAGGAC 
AGACCTGAGG GGTGTGAGGA GTGTGGATGC TTCAGACCTG GGACCAGCAG TGGACTGGGC 
ACTTGTGATA TGGCCATAGG TCAGTGTGCC TGCAAAGCTA ACGTCCAGGG GAGGATCTGT 
ATGGACTGTA GGGATGGAAG CTTTGGGATG TCGGAGGTGG ACAGTCTCGG ATGTCAGGGT 
TGCAGTTGTG ACGTAGGTGG GGCAGTGACA GATGTGTGTG ACAAGGCCAC AGGACAGTGC 
GTGTGCAGGG AGGGGATGGA GGGGCGGCAG TGTGACGTCC CCACCCAGGG GCAGTACGTC 
CCGGGGCTGT ACCAGTACAG GTACGAGGCC GAGGATGCAG ACACACCCCG CGGCATGCCC 
GTCAGGTTTG GGTACTCTCC AAAGGATTTC CCAGGGTTCA GCTGGAGGGG ATATGCAATC 
ATGAATACCG TCCAGCCAAG GATAATCCTG TCAGTCCTAG TTGACCGCCC TGCGCTGTAC 
CGTGTGGTTC TGTCATACGT CAACCCGGGA CAGGAGACTG TCATTGGCAA CATTCGAGCA 
AACCCGCTGT CTCAGTCAGC TGATGTTGTT TCCCAGCAGA GCAGTGAGAT AGTGTTTAAC 
CCCACCACCA CCCCGACAAC TGTCACTGTG GTGGGGAACA GTTTCATCAT GCCCTTCGTG 
CTCAACCCAG GACAATGGAG CTTCACCATT GAGGTGTCAA GGGTCCTGTT GGACTACATG 
GTGCTGCTGC CTGCAGCCTA CTTTGAGGCC CCACTGCTGC AGCTGAAGGT GGAGGTCCCC 
TGCAAGTATG AGATTCCAGA GGACCCCAAA GACCACTGCC TGATGTACAC CCATCTGCCC 
CTGGAAGACT TCACCACAGT CCAGGCAGAG TCTGGGTTCT TCCGCCCGCA GCAGGGCGAT 
GATGACATGA ACAACACCCT GTCTGTCCTG GGAGACAAGA TCGCTACACA GTACATCCAG 
CCCTCCAAGT TCTACCCACT CATGGCACTG TTGGATCACA ATCAGATGGT TCTACTTCTG 
GAGCTGACCC TGCCCAAGGC TGGCGAGTAT GTGTTTGTGA TCGAGTATGC CAACTTTGAG 
ACACGGGCAC AGACCCTGCA GATCCGTGTG GACAAGACAG AGGGCAAGAA TCAAGGCATC 
TTCAACATCA ACACCTGCAA CTTTAGTTTC CGCTGTCGTG CTGTTGGGAT TGACGACAAC 
GGGAGAGAGG CACGCTTCAA GGTTACCTCA AAGTCAACCA CCATCAGTCT GTCTGGAGAT 
AAGCTCAGAG TTGCCATTGA CTCACTGACT GCTATCCCTG TGGAATCATG GGACCCAGAT 
TACATCATTC CCAAACCTCA GTGTGTGGCC AGTTACGGGC CATTCTCCAA TCACAAGTGT 
GTCCAGTCCA TGTACACAGA ACCAGTCGGA ACAGTAAGGA TCATGCCGAG TTCTGGACAG 
GACTCGATGC GTCGCCAGCC TCTCCTGGTC CTGTCCAACT CCAAACTCTT CCTGCTGGAC 
AGGGAACAGA ACCAACTTGA GTTTAGTGGC CGTCTCCTCT CAGCTGGTAG AATGGTGTTC 
CTGCTCCACT ACTTCCAGCC TGAGAGTACA GGGTACACTG CACAGGTCAA GGTCACAACA 
CCGCTCAGGC AGGAGATTCA AGGAGTTATC CAGGTTGACT ACTGTCCCCA CAGTGCCGGC 
TGTCGTGCCA TCATGCTTTT CTCAGGTCTG GGACAGGAGG TGGACATCAC CCAGCCTGAC 
ATCAGGCTCA TGATCACTGT TCCTGCAGAC AGAACCATCT GGGTGGACCA TGTTGTGGCT 
ATTCCAGAGT TCAGCTACAC CCCCTCCCTT CTGATGGACA GCAGGATTGA CAAGTCTGGT 
GACTTCATCA AGCAGTGTGG CAAATTTGAC TTCAACCTCA ATGACAGGGA CCTGACTCCC 
TTCTGCCGGA AGTCCATCAT GTCACTGTCT GCCCACTACA ACGATGGGGC AACCCCCTGC 
AACTGTAATC CCCGGGGCTC TTCCAGCCAG ACCTGCTCCC CCCTGGGTGG ACAGTGTCCC 
TGTAAGAACA ACATCGTGGG GCGCAGCTGT GATGTCTGTG CTACTGGGTA CTTTGGCTTC 
CCTGACTGCA GAGCCTGCCC ATGTGGAGAG AACCGCCTGT GTGACCCGGT GACGGGAGAG 
TGCATCTGTC CGCCACGTTC CCGCCGGCCT GAGTGTACCG TCTGTGAGGA GGGGACGTAC 
GGGTACCACC CACTGCTGGG ATGTGAGCCA TGTGACTGTG ATGCCATGGG TATCCTGGAC 
CTGAACTACC CATCATGTAA CAGGAACACA GGGCAGTGCA GCTGCAAGCC AAACGTAGGA 
GGCCGACGTT GTGACCGCTG CCTGCCAGGC TACTACAACT TCCCCTACTG CTCCCCCTGT 
AGCTGTAGTG CTGATGGGGT CACTGAGGAT GTGTGTGACC AGAGAACAGG AAGGTGTCTG 
TGTAAGACCA ATGTGGATGG CGTGAGGTGT GACCAGTGTA AGGCCAACAC CTACCACCTG 
TCAGCCAGGA ACCCTGAGGG ATGCCACAGC TGCTTCTGCT TCGGTGCCAC ACCTTACTGT 
AGGAGCAGTG ACTTGCATTA CACAGAGCTT TTGGACATGG AGGACTGGAC CGTGTCCAAC 
CAGGTTGGGT CAGAGGTCAT GCGGTTTAAC GACAAGGTGG AGGTGAAGCT GGGAGAACTG 
ATCCAACCAG ACGAGCCCGT GTACTGGGTG GCACCAGGCA TCTACCTCGG CAACTTGGTA 
ACATCATATG GTGGGAGCCT CACCTATACT GTAGAGTACC AGGTGACTAA GACCGCCCTG 
ATGGCCAGGA TGCGCCCCAC CAGGCTGCCT GATGTGCTCC TATCTGGTAA TGACATGACC 
ATCATGTACT CCCACCCTGT CATCCCTGAG CCTGATGAGC CCCTCACTGT TACTGTGGAC 
CTGCTGGAGG GTAACTTTGT TCACAGTAAC AGTAAGGGTG CCGTGAGCCG GGAGGATCTG 
CTGATGGTAC TGGCTGATCT CCAGGCACTG CTCATCAGGG CTTCCTACAG GGAGAAGATT 
CTCAGTGCTA CTATTGAGTC AGTAAAGCTG AGCATCACGA CCCAAACCTT CTCTGGCAAG 
CCTGCCTTGG ATGTGGAGTA CTGTAACTGT CCATATGGAT ACAAAGGGAC TTCATGTCAG 
GAGTGTGATG AGGGTTACTA CAGGTCTAAG ACTGGTCCGT ACCTGGGCCA GTGTGTGAAG 
TGTGAGTGTT ACGGGCATGC CAGCAGCTGT GATGAGAATG GAGCATGCAT TGACTGTCAG 
CATCACACTA TGGGAGAGAG GTGTGACAAG TGCATGACTG GTTACTATGG TGAGCCCAGG 
AGGGGCACAC CCGACGACTG TCAGATCTGT GCCTGCCCCA TGGCAACAGA AGACAACAAC 
TTTGCCTCAG AGTGTCAGCC ACTTGCAGGT GGACAGTTCC AGTGCACCTG TCTGCCAGGA 
TATGATGGAC CAAACTGTGA CAGATGTGCT ATTGGCTTCT ATGGGAAGCC CAGTGAGGTG 
GGCGGGTTCT GTCAGCGCTG TGACTGCAGT GGGAATGTCC GGCTGACCAA GGTGGGGATG 
AAGAATGTGG AACAGTGTGA CGCAGAGACA GGACAGTGTG TGGACTGTCT GGACAACACC 
GGGGGCCGCA ACTGTCAGAA GTGTGCTGCT GGGTTCTATG GAGATGCCAT CAAGACCAGG 
AGTTGTTCAA AATGCAGGAA CTGTGATGTT TGCGGCACAG TACCTGATGT GTGCGATGAC 
AAGACGGGCA AGTGTCTGTG TCGGGACCAT GTGGTAGGGG ACAACTGTGA TCAGTGTGAG 
GAGAACCACT ATGGGTTTGA CTCGTGCCAA GGCTGCCAGG CCTGTGACTG TTCAGCGGAC 
GGTTCCTTGG CCAAGTCCTG TCACCCCAAG ACTGGTCAGT GCACCTGTAA GCCGGGTGTG 
ACCGGCCGGC GCTGTGACCG GTGTGAGGCC AAACACTCCA ACTTCACCAG TAGTGGATGC 
AGAGCTTGCA ACTGTCCAAA GGATGGACCA TGCGACCCAC AGACGGGGGA GTGCAGGTGT 
CCTGAGGGAC TGACAGGGAC AGACTGTGAG AGCTGCAAAA ATGAAACCCT CCTGTACACC 
CCCAGAGGGT GCTGGGGTGA GTTGGTCCAC TTGAATGTGT CAGACTGCAA TATGTTCACA 
TACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_83576 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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