BCIN_12g04860 cDNA ORF clone, Botrytis cinerea B05.10

Gene
Clones
gRNAs

Summary

Gene Symbol	BCIN_12g04860
Entrez Gene ID	5434405
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Botrytis cinerea B05.10

mRNA and Protein(s)

mRNA	Protein	Name
XM_001553849.1	XP_001553899.1	hypothetical protein
XM_001553849.2	XP_001553899.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Van Kan JA, Stassen JH, Mosbach A, Van Der Lee TA, Faino L, Farmer AD, Papasotiriou DG, Zhou S, Seidl MF, Cottam E, Edel D, Hahn M, Schwartz DC, Dietrich RA, Widdison S, Scalliet G
Molecular plant pathology18(1)75-89(2017 01)

Genome update of Botrytis cinerea strains B05.10 and T4.
Staats M, van Kan JA
Eukaryotic cell11(11)1413-4(2012 Nov)

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH, Dickman M
PLoS genetics7(8)e1002230(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BCIN_12g04860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCIN_12g04860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBc04845	XM_001553849.2 Latest version!	Botrytis cinerea B05.10 hypothetical protein (BCIN_12g04860), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBc04845	XM_001553849.1	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07459) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCIN_12g04860 ( XM_001553849.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001553899.1 Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07459) partial mRNA.

Price & Availability↑

CloneID	OBc04845
Clone ID Related Accession (Same CDS sequence)	XM_001553849.1 , XM_001553849.2
Accession Version	XM_001553849.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1203868800000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001814530). COMPLETENESS: incomplete on both ends.

ATGGCAGAAT TAGTTTTCCC TCCCGTTGCC GCCCCAATCC TCAATGGCAC TGATGAAGGA 
CTCATCAGTT TGGAATTGAA GGATGGCACA ATTTATCAAG GATATAGTTT TGGTGCGCAG 
AAGAGTGTCG CGGGAGAATT GGTCTTCCAG ACAGGTATGG TGGGGTACCC AGAATCCATC 
ACTGACCCTT CATACCGTGG ACAAATCTTG GTCATTACGT TCCCTTTGGT GGGCAATTAT 
GGTGTACCAT CTCGAGAAAC CATGGATGAA CTCCTCAAGG ATCTGCCAGC GCATTTCGAA 
GCATCACAGA TCCACATCGC AGGTTTGATT TGCGCTTCAT ACGCAGGCGA AGAGTTTTCT 
CATTTCCTCG CAACCTCTTC TTTGGGAGCA TGGTTGAAAG AGCAAGGTGT CCCAGCAATC 
TATGGTGTTG ACACCAGAGC TTTAACCAAG AGAATACGAG AAGAAGGTAG TATGCTTGGA 
CGAATGTTGT TGCAAAAGAA CATCCCTGCC AGTGGAAACG ACAACATCTC TGCTTTATCT 
ACAAAGGATT GGAGATCATC ATTCGAAGCA ATTGATTGGG TCGACCCCAA TGCCAAGAAC 
CTTGTCGCAG ATGTATCAAT TCGCGAGCCA AAATTATACA AACCTGATCC TTCGAATGCT 
TTGAAACATC CTTCTGGTAG ACCTATACGT GTGTTGTGTT TGGACGTTGG TTTGAAATAC 
AACCAGCTGC GTTGTCTTGT AAAGCGTGGT GTGGAAGTTC TCGTCTGCCC ATGGGATTAT 
GATTTCCCTG CTCTCGCCGG CAAGGATTAT GACGGTCTAT TCATCTCCAA CGGTCCTGGT 
GATCCAGCCA TGATGGATAA AACTGTCAAG CACATTCAAT CTGCCTTGAA GGAGAACCGT 
ACACCAATTT TCGGTATCTG TCTTGGACAT CAACTTCTCG CTAGGGCTGC TGGAGCTCAA 
ACCGAAAAGA TGAAGTTCGG TAACCGTGGA CATAACATTC CATGCACAAA CATGTTGACC 
GGAAAATGTC ATATTACTTC CCAAAACCAC GGTTTCGCTG TCCAAACTAG CTCCTTGCCT 
TCAGAATGGG AGGAACTTTT CGTTAATGCC AACGATGGAA GTAACGAGGG TATCAGACAT 
GTCAACAGGC CATACTTCAG TGTTCAATTC CATCCAGAAA GTACTCCTGG ACCAAGGGAT 
ACTGAATTCC TCTTTGATGT CTTCATCAAC ACTATCCAAA AGACTCTCGA TGACGAAAAA 
CTTCTTTCCG CACCAGTTGT CTTCCCAGGT GGTGAGAAAG CTGAGAATGA GAAGCTTACG 
CCTAAGGTCA ACGTCAAGAA AGTCCTTGTT TTGGGTAGTG GTGGTCTTAG TATCGGTCAA 
GCAGGCGAAT TCGATTACTC TGGAAGTCAA GCTATCAAAG CTCTTAAAGA GGAAGGAATC 
TATACCATCT TGATCAACCC TAACATCGCT ACAATTCAAA CTTCCAAAGG ATTGGCTGAT 
AAGGTCTATT TCCTTCCCGT CACAGCTGAT TTTGTTCGCA AAGTCATCAA GCACGAGAAG 
CCTGATGCAA TTTACGTCAC ATTTGGTGGC CAAACTGCTT TATCTGTTGG TATTCAACTC 
AAGGATGAAT TCGAAGAGCT CGGAGTTCGC GTCCTCGGTA CCCCAATTTC CACTGTCATC 
ACTACTGAAG ATCGAGAGTT GTTCGCTCGC AGTATGGAGT CGATTGGTGA GAAATGCGCC 
AAATCCGCTT CAGCGAACAA CATCGATGAA GCTATGCATG TCGTGAAGGA CATTGGATTC 
CCAGTCATCG TTCGCGCTGC ATATGCTTTG GGAGGTCTTG GAAGTGGTTT TGCAGACAAT 
GAGGAAGAAT TACTTGAACT CTGCAACAAA GCTTTCGCCG CCAGTCCTCA AGTTTTGATC 
GAGCGAAGTA TGAAGGGATG GAAGGAAATT GAGTACGAGG TTGTCCGTGA TTGTCGTGAC 
AACTGTATCA CAGTTTGTAA CATGGAGAAC TTCGATCCTC TGGGTATTCA CACTGGAGAC 
TCCATTGTTG TTGCTCCTTC TCAAACATTA TCCGATGAGG ATTACAACAT GTTGAGAACC 
ACAGCTGTTA ACGTCATTCG CCATCTCGGA GTTGTTGGAG AATGTAACAT TCAATACGCA 
CTCAACCCTG ACTCGAAGGA ATACTGTATT ATCGAGGTCA ACGCTAGATT GTCCAGATCT 
TCAGCCCTCG CATCCAAGGC TACTGGATAT CCATTGGCTT TCATCGCCGC AAAGCTCGGT 
CTTAACATCC CACTCAACGA AATCAAGAAC TCAGTCACTC AACAGACCTG CGCTTGTTTC 
GAACCATCTT TGGATTATGT TGTTGTTAAG ATGCCACGTT GGGATTTGAA GAAGTTCACT 
CGTGTTTCTA CACAACTTGG TTCATCAATG AAGAGTGTCG GTGAAGTTAT GAGTATCGGA 
AGAACTTTCG AAGAAGCTAT TCAGAAAGCA ATCCGTGCTA TTGATTTCCA TAACTTGGGT 
TTCAGTGCAA CAAAGGCATT AATGTCAATT GATGATGAAC TTCAAACGCC TTCCGATCAA 
CGTCTCTTTG CAATCGCTAA TGCCATGCAA TCTGGTTACT CTGTTAACAA GATTTGGGAA 
ATGACAAAGA TTGACAAATG GTTCTTGAAC AAGCTCAAGG GATTGAATGA CTTCAGCAAG 
CACATGTCAA CCTTCAACAC TAGCACCATC TCATATGACC AAATCAGACA AGCCAAACAA 
CTTGGATTCT GCGATAGACA ATTGGCTAAA TTCTGGGATT CTACAGAACT TGCTGTTAGA 
TTGTTGAGAA CCGAAGCTGG TATTGTCCCA GTTGTTAAGC AAATTGATAC CGTTGCTGCC 
GAGTTCGAGG CCCACACCAA TTATCTTTAC ATGACCTACA ACGGATCTGA GAGCGATATC 
TCTTTCAATG ACCACGGTGT CATGGTCCTC GGTTCTGGAG TTTACAGAAT TGGTTCTTCC 
GTCGAATTCG ATTGGTGTTC CGTTCGTGCT ATTAGAACTC TTCGGGAATC TGGTTTCAAG 
ACTGTCATGG TCAACTATAA CCCTGAGACT GTCAGTACCG ATTATGATGA AGCCGACAGA 
TTGTACTTTG AGAACATCAA CCTCGAAACC ATCCTCGACA TTTACGAATT GGAGTCTTCA 
AGTGGTGTTC TCATTGCTAT GGGAGGTCAA ACACCAAACA ACATTGCTCT CCCACTTCAC 
CGACAGAATG TCAAGATTCT TGGAACATCA CCAGAAATGA TTGATACTGC TGAGAATCGT 
TTCAAGTTCT CTCGTATGCT TGATGGCATC CATGTTGATC AACCAACATG GAAAGAGTTG 
ACTAGTTTCG AGGATGCTAA AACATTCTGT AACAAGGTCT CTTATCCAGT GCTCGTTAGA 
CCTTCATATG TGCTTTCAGG TGCAGCTATG AACACTGTTT ACTCCGAACC AGATCTTGAA 
GCTTATCTTG CTCAAGCTGT TGAAGTTTCA AGAGATCATC CAGTCGTTAT CACCAAATAT 
ATCGAAAATG CAAAGGAAAT TGAAATGGAC GCTGTTGCAA AGGATGGTGT TATGGTTGGT 
CACTTTATCT CTGAGCATGT TGAAAACGCC GGTGTACATT CTGGTGATGC TACTTTGATT 
TTACCACCTC AAGATCTCGA CCCTACCACT ATCAAGAGAA TTGAGGAAGC TACTCGCTTG 
ATTGGTAAAG CTCTTAACAT CACTGGTCCT TATAACATTC AATTCATCGC CAAGGACAAT 
GATATTAAGG TCATCGAATG TAATGTTCGT GCATCTAGAT CGACTCCATT CGTTTCAAAG 
GTCATGGGTG TTGATCTTAT TGAAATGGCT ACTAAAGCCA TTATGGGTGT ACCATTCATG 
CCATACCCAC CTACCGAATT ACCTGCAGGC TGCGTTGGTG TTAAAGTACC TCAATTCAGT 
TTCTCTCGAC TTTCTGGTGC TGATCCAGTT CTTGGTGTCG AGATGGCTTC TACTGGTGAA 
GTTGCTTGTT TCGGTCGTGA TAAGTATGAG GCTTACATCA AAGCATTGAT CTCTACTGGA 
TTCAAATTAC CAAAGCAAAA CATTCTCCTT TCTATTGGTT CATACAAGGA CAAGAAGGAA 
ATGCTTCCAT CTGTTGTCAA GCTTCAAAAG TTGGGTTACA AACTTTTCGC TACAGCCGGT 
ACCGCAGATT TCTTACAAGA ACATGATGTC CCAGTTCAGT ACCTCGAAAT TCTCGGAAAG 
GGTGAGGATG ATCAAAAATC GGAATATTCT TTGACTCAAC ATCTTGCCAA TAACATGATT 
GATCTTTATA TCAATCTTCC TTCCAGCAAC AGATATAGAA GACCTGCCAA CTACATGAGT 
AAGGGTTACC AAACTAGACG TATGGCTGTC GATTATCAAA CACCTTTGGT CACCAACGTC 
AAGAACGCCA AGATCCTTAT CGAAGCCATC GCAAGACATT ATGATATGGA AGTCAGTGGA 
TTCGATGCAC AAACTAGTCA CAGAACTGTT GTTCTTCCTG GTCTTATCAA CATTGCATCT 
TTCGTTCCTG GCATCACCAC TCCTGGCAGC CGTGATTTCC AAACTGTCAC CAAAGCATCA 
ATTGCATCTG GTTTCAGTAT GATTCGTGTT ATGCCTCTTG GTCTTGATGG ATCAGTCACT 
GATGCTAGAG CATTGAAGGT AGCTCAACTT AACAGCAAAC TTGGTTCATC TTGCGATTAC 
AATTTCTCTG TTGCCGCAAC TTCATCTAAC GCCGATCAAA TTAGTCAAGT CACTGGTGAA 
GTTGGCTCTC TCTTCATCCC ATTCAATCAC TTGTCCGATA ATATCAGCAA GGTAGCTGCT 
GTTACTGCTC ATTTTGAATC TTGGCCATCT TTCAAGCCAA TCATCACTGA TGCAAAGATG 
ACCGATCTTG CATCAATTCT ACTTCTCGCA AGTCTCCATA GCCGAAAGAT TCACATCACC 
AATGTCACCA CCAAGGATGA TATTGGATTG ATTGATTTGG CTAAGCAAAA AGGTCTTCAA 
GTCACTTGTG ATGTTTCAGT GTACGCCCTT TTCTTCAGCC AAGATGATTA CCCTGAAGCT 
TCTTTCCTTC CAACTGCCAA GGACCAAGAA GCATTATGGG AACACATGAA CATCATTGAT 
GTCTTTTCTA TTGGTAGCTT ACCATATCAA CTTGCAACTC TTGCAAAGGC AGAGGCAGAC 
GCCGCTGTTG GTATTGCTGA CACTTTGCCT CTACTGTTCT CAGCTGTTGC AGATGGTAGA 
CTCACAGTCG ATGATATTAA GGCTCGTCTC CATGACAATC CAAAGGAGAT TTTCGAAATT 
CACGATCAAG TCGGAGCTTC CGTAGAGGTT GAGATTGATA GACCACACAC TGTCAAGCCA 
GGAACAGTTT GGTCGCCACT TGTTGGACAG GTCACAAGAG GAGCTGTCCA AAGAGTCATC 
TTCCAAGATA AGACTGCTTG TCTAGATGGT TTACCAACTC AAGATGCTAT TGATGGTAAA 
GATATGTCAT CTCATCTTAT TGATTTACAT ACCACAGTAC CACAATCACC ACTTGTCAAG 
GCTAATCTTG GATCCCCAGC TATGGGACCA AGACCTGATA GCTCTCATGG ATTGAGAAAG 
TTCTCTTCAT TTGCTGGTGC ATCATCTTCA GCTATGCGAC CTGCAACTCG TTCAAGGTTC 
TTGGACGGTG CTGCCAGCCA AGCACCACAA CTTGGTTCAA CAGCTGAAGA TTTGGGTGCT 
CCATTATACC CTCAACCAAC AATATCACCA TCTCTACAAA CCCTCCTCGG CCAATCGCCA 
TTCAAGAATC AACACGTTCT TTCGGTCAAT CAATTCTCCC GTCAAGATCT TCATCTTCTT 
TTCTCTGTTG CTCAAGAAAT GAGATTGGGT GTTCAACGCG AAGGTGTTCT TTCTATTCTC 
AAGGGACGTC TTCTTTGCAC AATGTTCTAC GAACCATCTA CTCGTACATC TGCATCATTT 
GATGCTGCTA TGCAAAGATT GGGTGGACGA ACAGTTGCAA TCGCTACTTC TCATTCTTCC 
ACTGTCAAGG GCGAATCTCT TGCAGATACC ATCCGCACTC TTGGTTGTTA TGGAGATGCT 
ATTGTCCTTC GTCACCCTGA AGAGAGTAGT GCTTCAACTG CTGCCAAATT TTCTCCAGTT 
CCAATTATCA ACGGAGGTAA TGGAAGCAAA GAACACCCAA CTCAAGCATT CTTGGATTTG 
TTCACAATCA GAGAAGAATT GGGTACTGTT GGTGGTCTTA CCATTACCTT TACTGGTGAT 
CTTAGATATG GTCGTACCGT TCACTCTCTC GTCAAGCTTC TTCAACATTA TGATGTTCAC 
ATCCAGCTTG TCTCCCCACA AGCTTTATCC CTTCCACAAG AGATCTTGGC ACTTATCAAA 
TCTAAGCCTG GTCAATTACT TTCAGTTTCT ACTGAGTTGA CCCCCGAGAT CCTTGCTAGA 
TCCGATGTCC TTTACTGTAC TCGTGTACAA AAGGAGCGAT TTGCCGATTT GGAAGAGTAT 
GAGAGATTGA AGGATAGTTT CATCATTGAT AACAAGACAT TGAGAAATGC AAAGAGTTCA 
ATGATTGTTA TGCATCCATT GCCGAGAAAT GCCGAGATTG GAGAGGAAGT GGATTTCGAT 
CAAAGAGCTG CATACTTTAG ACAGATGAGA TATGGCCTTT ACTGTCGCAT GGCATTGTTG 
GCTTTGGTAT TAGCCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001553899.1
CDS	1..6741
Translation	MAELVFPPVAAPILNGTDEGLISLELKDGTIYQGYSFGAQKSVAGELVFQTGMVGYPESITDPSYRGQILVITFPLVGNYGVPSRETMDELLKDLPAHFEASQIHIAGLICASYAGEEFSHFLATSSLGAWLKEQGVPAIYGVDTRALTKRIREEGSMLGRMLLQKNIPASGNDNISALSTKDWRSSFEAIDWVDPNAKNLVADVSIREPKLYKPDPSNALKHPSGRPIRVLCLDVGLKYNQLRCLVKRGVEVLVCPWDYDFPALAGKDYDGLFISNGPGDPAMMDKTVKHIQSALKENRTPIFGICLGHQLLARAAGAQTEKMKFGNRGHNIPCTNMLTGKCHITSQNHGFAVQTSSLPSEWEELFVNANDGSNEGIRHVNRPYFSVQFHPESTPGPRDTEFLFDVFINTIQKTLDDEKLLSAPVVFPGGEKAENEKLTPKVNVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVTADFVRKVIKHEKPDAIYVTFGGQTALSVGIQLKDEFEELGVRVLGTPISTVITTEDRELFARSMESIGEKCAKSASANNIDEAMHVVKDIGFPVIVRAAYALGGLGSGFADNEEELLELCNKAFAASPQVLIERSMKGWKEIEYEVVRDCRDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPDSKEYCIIEVNARLSRSSALASKATGYPLAFIAAKLGLNIPLNEIKNSVTQQTCACFEPSLDYVVVKMPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRAIDFHNLGFSATKALMSIDDELQTPSDQRLFAIANAMQSGYSVNKIWEMTKIDKWFLNKLKGLNDFSKHMSTFNTSTISYDQIRQAKQLGFCDRQLAKFWDSTELAVRLLRTEAGIVPVVKQIDTVAAEFEAHTNYLYMTYNGSESDISFNDHGVMVLGSGVYRIGSSVEFDWCSVRAIRTLRESGFKTVMVNYNPETVSTDYDEADRLYFENINLETILDIYELESSSGVLIAMGGQTPNNIALPLHRQNVKILGTSPEMIDTAENRFKFSRMLDGIHVDQPTWKELTSFEDAKTFCNKVSYPVLVRPSYVLSGAAMNTVYSEPDLEAYLAQAVEVSRDHPVVITKYIENAKEIEMDAVAKDGVMVGHFISEHVENAGVHSGDATLILPPQDLDPTTIKRIEEATRLIGKALNITGPYNIQFIAKDNDIKVIECNVRASRSTPFVSKVMGVDLIEMATKAIMGVPFMPYPPTELPAGCVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRDKYEAYIKALISTGFKLPKQNILLSIGSYKDKKEMLPSVVKLQKLGYKLFATAGTADFLQEHDVPVQYLEILGKGEDDQKSEYSLTQHLANNMIDLYINLPSSNRYRRPANYMSKGYQTRRMAVDYQTPLVTNVKNAKILIEAIARHYDMEVSGFDAQTSHRTVVLPGLINIASFVPGITTPGSRDFQTVTKASIASGFSMIRVMPLGLDGSVTDARALKVAQLNSKLGSSCDYNFSVAATSSNADQISQVTGEVGSLFIPFNHLSDNISKVAAVTAHFESWPSFKPIITDAKMTDLASILLLASLHSRKIHITNVTTKDDIGLIDLAKQKGLQVTCDVSVYALFFSQDDYPEASFLPTAKDQEALWEHMNIIDVFSIGSLPYQLATLAKAEADAAVGIADTLPLLFSAVADGRLTVDDIKARLHDNPKEIFEIHDQVGASVEVEIDRPHTVKPGTVWSPLVGQVTRGAVQRVIFQDKTACLDGLPTQDAIDGKDMSSHLIDLHTTVPQSPLVKANLGSPAMGPRPDSSHGLRKFSSFAGASSSAMRPATRSRFLDGAASQAPQLGSTAEDLGAPLYPQPTISPSLQTLLGQSPFKNQHVLSVNQFSRQDLHLLFSVAQEMRLGVQREGVLSILKGRLLCTMFYEPSTRTSASFDAAMQRLGGRTVAIATSHSSTVKGESLADTIRTLGCYGDAIVLRHPEESSASTAAKFSPVPIINGGNGSKEHPTQAFLDLFTIREELGTVGGLTITFTGDLRYGRTVHSLVKLLQHYDVHIQLVSPQALSLPQEILALIKSKPGQLLSVSTELTPEILARSDVLYCTRVQKERFADLEEYERLKDSFIIDNKTLRNAKSSMIVMHPLPRNAEIGEEVDFDQRAAYFRQMRYGLYCRMALLALVLAP

Target ORF information:

RefSeq Version	XM_001553849.1
Organism	Botrytis cinerea B05.10
Definition	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07459) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001553849.1

ORF Insert Sequence:

ATGGCAGAAT TAGTTTTCCC TCCCGTTGCC GCCCCAATCC TCAATGGCAC TGATGAAGGA 
CTCATCAGTT TGGAATTGAA GGATGGCACA ATTTATCAAG GATATAGTTT TGGTGCGCAG 
AAGAGTGTCG CGGGAGAATT GGTCTTCCAG ACAGGTATGG TGGGGTACCC AGAATCCATC 
ACTGACCCTT CATACCGTGG ACAAATCTTG GTCATTACGT TCCCTTTGGT GGGCAATTAT 
GGTGTACCAT CTCGAGAAAC CATGGATGAA CTCCTCAAGG ATCTGCCAGC GCATTTCGAA 
GCATCACAGA TCCACATCGC AGGTTTGATT TGCGCTTCAT ACGCAGGCGA AGAGTTTTCT 
CATTTCCTCG CAACCTCTTC TTTGGGAGCA TGGTTGAAAG AGCAAGGTGT CCCAGCAATC 
TATGGTGTTG ACACCAGAGC TTTAACCAAG AGAATACGAG AAGAAGGTAG TATGCTTGGA 
CGAATGTTGT TGCAAAAGAA CATCCCTGCC AGTGGAAACG ACAACATCTC TGCTTTATCT 
ACAAAGGATT GGAGATCATC ATTCGAAGCA ATTGATTGGG TCGACCCCAA TGCCAAGAAC 
CTTGTCGCAG ATGTATCAAT TCGCGAGCCA AAATTATACA AACCTGATCC TTCGAATGCT 
TTGAAACATC CTTCTGGTAG ACCTATACGT GTGTTGTGTT TGGACGTTGG TTTGAAATAC 
AACCAGCTGC GTTGTCTTGT AAAGCGTGGT GTGGAAGTTC TCGTCTGCCC ATGGGATTAT 
GATTTCCCTG CTCTCGCCGG CAAGGATTAT GACGGTCTAT TCATCTCCAA CGGTCCTGGT 
GATCCAGCCA TGATGGATAA AACTGTCAAG CACATTCAAT CTGCCTTGAA GGAGAACCGT 
ACACCAATTT TCGGTATCTG TCTTGGACAT CAACTTCTCG CTAGGGCTGC TGGAGCTCAA 
ACCGAAAAGA TGAAGTTCGG TAACCGTGGA CATAACATTC CATGCACAAA CATGTTGACC 
GGAAAATGTC ATATTACTTC CCAAAACCAC GGTTTCGCTG TCCAAACTAG CTCCTTGCCT 
TCAGAATGGG AGGAACTTTT CGTTAATGCC AACGATGGAA GTAACGAGGG TATCAGACAT 
GTCAACAGGC CATACTTCAG TGTTCAATTC CATCCAGAAA GTACTCCTGG ACCAAGGGAT 
ACTGAATTCC TCTTTGATGT CTTCATCAAC ACTATCCAAA AGACTCTCGA TGACGAAAAA 
CTTCTTTCCG CACCAGTTGT CTTCCCAGGT GGTGAGAAAG CTGAGAATGA GAAGCTTACG 
CCTAAGGTCA ACGTCAAGAA AGTCCTTGTT TTGGGTAGTG GTGGTCTTAG TATCGGTCAA 
GCAGGCGAAT TCGATTACTC TGGAAGTCAA GCTATCAAAG CTCTTAAAGA GGAAGGAATC 
TATACCATCT TGATCAACCC TAACATCGCT ACAATTCAAA CTTCCAAAGG ATTGGCTGAT 
AAGGTCTATT TCCTTCCCGT CACAGCTGAT TTTGTTCGCA AAGTCATCAA GCACGAGAAG 
CCTGATGCAA TTTACGTCAC ATTTGGTGGC CAAACTGCTT TATCTGTTGG TATTCAACTC 
AAGGATGAAT TCGAAGAGCT CGGAGTTCGC GTCCTCGGTA CCCCAATTTC CACTGTCATC 
ACTACTGAAG ATCGAGAGTT GTTCGCTCGC AGTATGGAGT CGATTGGTGA GAAATGCGCC 
AAATCCGCTT CAGCGAACAA CATCGATGAA GCTATGCATG TCGTGAAGGA CATTGGATTC 
CCAGTCATCG TTCGCGCTGC ATATGCTTTG GGAGGTCTTG GAAGTGGTTT TGCAGACAAT 
GAGGAAGAAT TACTTGAACT CTGCAACAAA GCTTTCGCCG CCAGTCCTCA AGTTTTGATC 
GAGCGAAGTA TGAAGGGATG GAAGGAAATT GAGTACGAGG TTGTCCGTGA TTGTCGTGAC 
AACTGTATCA CAGTTTGTAA CATGGAGAAC TTCGATCCTC TGGGTATTCA CACTGGAGAC 
TCCATTGTTG TTGCTCCTTC TCAAACATTA TCCGATGAGG ATTACAACAT GTTGAGAACC 
ACAGCTGTTA ACGTCATTCG CCATCTCGGA GTTGTTGGAG AATGTAACAT TCAATACGCA 
CTCAACCCTG ACTCGAAGGA ATACTGTATT ATCGAGGTCA ACGCTAGATT GTCCAGATCT 
TCAGCCCTCG CATCCAAGGC TACTGGATAT CCATTGGCTT TCATCGCCGC AAAGCTCGGT 
CTTAACATCC CACTCAACGA AATCAAGAAC TCAGTCACTC AACAGACCTG CGCTTGTTTC 
GAACCATCTT TGGATTATGT TGTTGTTAAG ATGCCACGTT GGGATTTGAA GAAGTTCACT 
CGTGTTTCTA CACAACTTGG TTCATCAATG AAGAGTGTCG GTGAAGTTAT GAGTATCGGA 
AGAACTTTCG AAGAAGCTAT TCAGAAAGCA ATCCGTGCTA TTGATTTCCA TAACTTGGGT 
TTCAGTGCAA CAAAGGCATT AATGTCAATT GATGATGAAC TTCAAACGCC TTCCGATCAA 
CGTCTCTTTG CAATCGCTAA TGCCATGCAA TCTGGTTACT CTGTTAACAA GATTTGGGAA 
ATGACAAAGA TTGACAAATG GTTCTTGAAC AAGCTCAAGG GATTGAATGA CTTCAGCAAG 
CACATGTCAA CCTTCAACAC TAGCACCATC TCATATGACC AAATCAGACA AGCCAAACAA 
CTTGGATTCT GCGATAGACA ATTGGCTAAA TTCTGGGATT CTACAGAACT TGCTGTTAGA 
TTGTTGAGAA CCGAAGCTGG TATTGTCCCA GTTGTTAAGC AAATTGATAC CGTTGCTGCC 
GAGTTCGAGG CCCACACCAA TTATCTTTAC ATGACCTACA ACGGATCTGA GAGCGATATC 
TCTTTCAATG ACCACGGTGT CATGGTCCTC GGTTCTGGAG TTTACAGAAT TGGTTCTTCC 
GTCGAATTCG ATTGGTGTTC CGTTCGTGCT ATTAGAACTC TTCGGGAATC TGGTTTCAAG 
ACTGTCATGG TCAACTATAA CCCTGAGACT GTCAGTACCG ATTATGATGA AGCCGACAGA 
TTGTACTTTG AGAACATCAA CCTCGAAACC ATCCTCGACA TTTACGAATT GGAGTCTTCA 
AGTGGTGTTC TCATTGCTAT GGGAGGTCAA ACACCAAACA ACATTGCTCT CCCACTTCAC 
CGACAGAATG TCAAGATTCT TGGAACATCA CCAGAAATGA TTGATACTGC TGAGAATCGT 
TTCAAGTTCT CTCGTATGCT TGATGGCATC CATGTTGATC AACCAACATG GAAAGAGTTG 
ACTAGTTTCG AGGATGCTAA AACATTCTGT AACAAGGTCT CTTATCCAGT GCTCGTTAGA 
CCTTCATATG TGCTTTCAGG TGCAGCTATG AACACTGTTT ACTCCGAACC AGATCTTGAA 
GCTTATCTTG CTCAAGCTGT TGAAGTTTCA AGAGATCATC CAGTCGTTAT CACCAAATAT 
ATCGAAAATG CAAAGGAAAT TGAAATGGAC GCTGTTGCAA AGGATGGTGT TATGGTTGGT 
CACTTTATCT CTGAGCATGT TGAAAACGCC GGTGTACATT CTGGTGATGC TACTTTGATT 
TTACCACCTC AAGATCTCGA CCCTACCACT ATCAAGAGAA TTGAGGAAGC TACTCGCTTG 
ATTGGTAAAG CTCTTAACAT CACTGGTCCT TATAACATTC AATTCATCGC CAAGGACAAT 
GATATTAAGG TCATCGAATG TAATGTTCGT GCATCTAGAT CGACTCCATT CGTTTCAAAG 
GTCATGGGTG TTGATCTTAT TGAAATGGCT ACTAAAGCCA TTATGGGTGT ACCATTCATG 
CCATACCCAC CTACCGAATT ACCTGCAGGC TGCGTTGGTG TTAAAGTACC TCAATTCAGT 
TTCTCTCGAC TTTCTGGTGC TGATCCAGTT CTTGGTGTCG AGATGGCTTC TACTGGTGAA 
GTTGCTTGTT TCGGTCGTGA TAAGTATGAG GCTTACATCA AAGCATTGAT CTCTACTGGA 
TTCAAATTAC CAAAGCAAAA CATTCTCCTT TCTATTGGTT CATACAAGGA CAAGAAGGAA 
ATGCTTCCAT CTGTTGTCAA GCTTCAAAAG TTGGGTTACA AACTTTTCGC TACAGCCGGT 
ACCGCAGATT TCTTACAAGA ACATGATGTC CCAGTTCAGT ACCTCGAAAT TCTCGGAAAG 
GGTGAGGATG ATCAAAAATC GGAATATTCT TTGACTCAAC ATCTTGCCAA TAACATGATT 
GATCTTTATA TCAATCTTCC TTCCAGCAAC AGATATAGAA GACCTGCCAA CTACATGAGT 
AAGGGTTACC AAACTAGACG TATGGCTGTC GATTATCAAA CACCTTTGGT CACCAACGTC 
AAGAACGCCA AGATCCTTAT CGAAGCCATC GCAAGACATT ATGATATGGA AGTCAGTGGA 
TTCGATGCAC AAACTAGTCA CAGAACTGTT GTTCTTCCTG GTCTTATCAA CATTGCATCT 
TTCGTTCCTG GCATCACCAC TCCTGGCAGC CGTGATTTCC AAACTGTCAC CAAAGCATCA 
ATTGCATCTG GTTTCAGTAT GATTCGTGTT ATGCCTCTTG GTCTTGATGG ATCAGTCACT 
GATGCTAGAG CATTGAAGGT AGCTCAACTT AACAGCAAAC TTGGTTCATC TTGCGATTAC 
AATTTCTCTG TTGCCGCAAC TTCATCTAAC GCCGATCAAA TTAGTCAAGT CACTGGTGAA 
GTTGGCTCTC TCTTCATCCC ATTCAATCAC TTGTCCGATA ATATCAGCAA GGTAGCTGCT 
GTTACTGCTC ATTTTGAATC TTGGCCATCT TTCAAGCCAA TCATCACTGA TGCAAAGATG 
ACCGATCTTG CATCAATTCT ACTTCTCGCA AGTCTCCATA GCCGAAAGAT TCACATCACC 
AATGTCACCA CCAAGGATGA TATTGGATTG ATTGATTTGG CTAAGCAAAA AGGTCTTCAA 
GTCACTTGTG ATGTTTCAGT GTACGCCCTT TTCTTCAGCC AAGATGATTA CCCTGAAGCT 
TCTTTCCTTC CAACTGCCAA GGACCAAGAA GCATTATGGG AACACATGAA CATCATTGAT 
GTCTTTTCTA TTGGTAGCTT ACCATATCAA CTTGCAACTC TTGCAAAGGC AGAGGCAGAC 
GCCGCTGTTG GTATTGCTGA CACTTTGCCT CTACTGTTCT CAGCTGTTGC AGATGGTAGA 
CTCACAGTCG ATGATATTAA GGCTCGTCTC CATGACAATC CAAAGGAGAT TTTCGAAATT 
CACGATCAAG TCGGAGCTTC CGTAGAGGTT GAGATTGATA GACCACACAC TGTCAAGCCA 
GGAACAGTTT GGTCGCCACT TGTTGGACAG GTCACAAGAG GAGCTGTCCA AAGAGTCATC 
TTCCAAGATA AGACTGCTTG TCTAGATGGT TTACCAACTC AAGATGCTAT TGATGGTAAA 
GATATGTCAT CTCATCTTAT TGATTTACAT ACCACAGTAC CACAATCACC ACTTGTCAAG 
GCTAATCTTG GATCCCCAGC TATGGGACCA AGACCTGATA GCTCTCATGG ATTGAGAAAG 
TTCTCTTCAT TTGCTGGTGC ATCATCTTCA GCTATGCGAC CTGCAACTCG TTCAAGGTTC 
TTGGACGGTG CTGCCAGCCA AGCACCACAA CTTGGTTCAA CAGCTGAAGA TTTGGGTGCT 
CCATTATACC CTCAACCAAC AATATCACCA TCTCTACAAA CCCTCCTCGG CCAATCGCCA 
TTCAAGAATC AACACGTTCT TTCGGTCAAT CAATTCTCCC GTCAAGATCT TCATCTTCTT 
TTCTCTGTTG CTCAAGAAAT GAGATTGGGT GTTCAACGCG AAGGTGTTCT TTCTATTCTC 
AAGGGACGTC TTCTTTGCAC AATGTTCTAC GAACCATCTA CTCGTACATC TGCATCATTT 
GATGCTGCTA TGCAAAGATT GGGTGGACGA ACAGTTGCAA TCGCTACTTC TCATTCTTCC 
ACTGTCAAGG GCGAATCTCT TGCAGATACC ATCCGCACTC TTGGTTGTTA TGGAGATGCT 
ATTGTCCTTC GTCACCCTGA AGAGAGTAGT GCTTCAACTG CTGCCAAATT TTCTCCAGTT 
CCAATTATCA ACGGAGGTAA TGGAAGCAAA GAACACCCAA CTCAAGCATT CTTGGATTTG 
TTCACAATCA GAGAAGAATT GGGTACTGTT GGTGGTCTTA CCATTACCTT TACTGGTGAT 
CTTAGATATG GTCGTACCGT TCACTCTCTC GTCAAGCTTC TTCAACATTA TGATGTTCAC 
ATCCAGCTTG TCTCCCCACA AGCTTTATCC CTTCCACAAG AGATCTTGGC ACTTATCAAA 
TCTAAGCCTG GTCAATTACT TTCAGTTTCT ACTGAGTTGA CCCCCGAGAT CCTTGCTAGA 
TCCGATGTCC TTTACTGTAC TCGTGTACAA AAGGAGCGAT TTGCCGATTT GGAAGAGTAT 
GAGAGATTGA AGGATAGTTT CATCATTGAT AACAAGACAT TGAGAAATGC AAAGAGTTCA 
ATGATTGTTA TGCATCCATT GCCGAGAAAT GCCGAGATTG GAGAGGAAGT GGATTTCGAT 
CAAAGAGCTG CATACTTTAG ACAGATGAGA TATGGCCTTT ACTGTCGCAT GGCATTGTTG 
GCTTTGGTAT TAGCCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCIN_12g04860 ( XM_001553849.2 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001553899.1 Botrytis cinerea B05.10 hypothetical protein (BCIN_12g04860), mRNA.

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CloneID	OBc04845
Clone ID Related Accession (Same CDS sequence)	XM_001553849.1 , XM_001553849.2
Accession Version	XM_001553849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523808000000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_037321). On Apr 17, 2018 this sequence version replaced XM_001553849.1.

ATGGCAGAAT TAGTTTTCCC TCCCGTTGCC GCCCCAATCC TCAATGGCAC TGATGAAGGA 
CTCATCAGTT TGGAATTGAA GGATGGCACA ATTTATCAAG GATATAGTTT TGGTGCGCAG 
AAGAGTGTCG CGGGAGAATT GGTCTTCCAG ACAGGTATGG TGGGGTACCC AGAATCCATC 
ACTGACCCTT CATACCGTGG ACAAATCTTG GTCATTACGT TCCCTTTGGT GGGCAATTAT 
GGTGTACCAT CTCGAGAAAC CATGGATGAA CTCCTCAAGG ATCTGCCAGC GCATTTCGAA 
GCATCACAGA TCCACATCGC AGGTTTGATT TGCGCTTCAT ACGCAGGCGA AGAGTTTTCT 
CATTTCCTCG CAACCTCTTC TTTGGGAGCA TGGTTGAAAG AGCAAGGTGT CCCAGCAATC 
TATGGTGTTG ACACCAGAGC TTTAACCAAG AGAATACGAG AAGAAGGTAG TATGCTTGGA 
CGAATGTTGT TGCAAAAGAA CATCCCTGCC AGTGGAAACG ACAACATCTC TGCTTTATCT 
ACAAAGGATT GGAGATCATC ATTCGAAGCA ATTGATTGGG TCGACCCCAA TGCCAAGAAC 
CTTGTCGCAG ATGTATCAAT TCGCGAGCCA AAATTATACA AACCTGATCC TTCGAATGCT 
TTGAAACATC CTTCTGGTAG ACCTATACGT GTGTTGTGTT TGGACGTTGG TTTGAAATAC 
AACCAGCTGC GTTGTCTTGT AAAGCGTGGT GTGGAAGTTC TCGTCTGCCC ATGGGATTAT 
GATTTCCCTG CTCTCGCCGG CAAGGATTAT GACGGTCTAT TCATCTCCAA CGGTCCTGGT 
GATCCAGCCA TGATGGATAA AACTGTCAAG CACATTCAAT CTGCCTTGAA GGAGAACCGT 
ACACCAATTT TCGGTATCTG TCTTGGACAT CAACTTCTCG CTAGGGCTGC TGGAGCTCAA 
ACCGAAAAGA TGAAGTTCGG TAACCGTGGA CATAACATTC CATGCACAAA CATGTTGACC 
GGAAAATGTC ATATTACTTC CCAAAACCAC GGTTTCGCTG TCCAAACTAG CTCCTTGCCT 
TCAGAATGGG AGGAACTTTT CGTTAATGCC AACGATGGAA GTAACGAGGG TATCAGACAT 
GTCAACAGGC CATACTTCAG TGTTCAATTC CATCCAGAAA GTACTCCTGG ACCAAGGGAT 
ACTGAATTCC TCTTTGATGT CTTCATCAAC ACTATCCAAA AGACTCTCGA TGACGAAAAA 
CTTCTTTCCG CACCAGTTGT CTTCCCAGGT GGTGAGAAAG CTGAGAATGA GAAGCTTACG 
CCTAAGGTCA ACGTCAAGAA AGTCCTTGTT TTGGGTAGTG GTGGTCTTAG TATCGGTCAA 
GCAGGCGAAT TCGATTACTC TGGAAGTCAA GCTATCAAAG CTCTTAAAGA GGAAGGAATC 
TATACCATCT TGATCAACCC TAACATCGCT ACAATTCAAA CTTCCAAAGG ATTGGCTGAT 
AAGGTCTATT TCCTTCCCGT CACAGCTGAT TTTGTTCGCA AAGTCATCAA GCACGAGAAG 
CCTGATGCAA TTTACGTCAC ATTTGGTGGC CAAACTGCTT TATCTGTTGG TATTCAACTC 
AAGGATGAAT TCGAAGAGCT CGGAGTTCGC GTCCTCGGTA CCCCAATTTC CACTGTCATC 
ACTACTGAAG ATCGAGAGTT GTTCGCTCGC AGTATGGAGT CGATTGGTGA GAAATGCGCC 
AAATCCGCTT CAGCGAACAA CATCGATGAA GCTATGCATG TCGTGAAGGA CATTGGATTC 
CCAGTCATCG TTCGCGCTGC ATATGCTTTG GGAGGTCTTG GAAGTGGTTT TGCAGACAAT 
GAGGAAGAAT TACTTGAACT CTGCAACAAA GCTTTCGCCG CCAGTCCTCA AGTTTTGATC 
GAGCGAAGTA TGAAGGGATG GAAGGAAATT GAGTACGAGG TTGTCCGTGA TTGTCGTGAC 
AACTGTATCA CAGTTTGTAA CATGGAGAAC TTCGATCCTC TGGGTATTCA CACTGGAGAC 
TCCATTGTTG TTGCTCCTTC TCAAACATTA TCCGATGAGG ATTACAACAT GTTGAGAACC 
ACAGCTGTTA ACGTCATTCG CCATCTCGGA GTTGTTGGAG AATGTAACAT TCAATACGCA 
CTCAACCCTG ACTCGAAGGA ATACTGTATT ATCGAGGTCA ACGCTAGATT GTCCAGATCT 
TCAGCCCTCG CATCCAAGGC TACTGGATAT CCATTGGCTT TCATCGCCGC AAAGCTCGGT 
CTTAACATCC CACTCAACGA AATCAAGAAC TCAGTCACTC AACAGACCTG CGCTTGTTTC 
GAACCATCTT TGGATTATGT TGTTGTTAAG ATGCCACGTT GGGATTTGAA GAAGTTCACT 
CGTGTTTCTA CACAACTTGG TTCATCAATG AAGAGTGTCG GTGAAGTTAT GAGTATCGGA 
AGAACTTTCG AAGAAGCTAT TCAGAAAGCA ATCCGTGCTA TTGATTTCCA TAACTTGGGT 
TTCAGTGCAA CAAAGGCATT AATGTCAATT GATGATGAAC TTCAAACGCC TTCCGATCAA 
CGTCTCTTTG CAATCGCTAA TGCCATGCAA TCTGGTTACT CTGTTAACAA GATTTGGGAA 
ATGACAAAGA TTGACAAATG GTTCTTGAAC AAGCTCAAGG GATTGAATGA CTTCAGCAAG 
CACATGTCAA CCTTCAACAC TAGCACCATC TCATATGACC AAATCAGACA AGCCAAACAA 
CTTGGATTCT GCGATAGACA ATTGGCTAAA TTCTGGGATT CTACAGAACT TGCTGTTAGA 
TTGTTGAGAA CCGAAGCTGG TATTGTCCCA GTTGTTAAGC AAATTGATAC CGTTGCTGCC 
GAGTTCGAGG CCCACACCAA TTATCTTTAC ATGACCTACA ACGGATCTGA GAGCGATATC 
TCTTTCAATG ACCACGGTGT CATGGTCCTC GGTTCTGGAG TTTACAGAAT TGGTTCTTCC 
GTCGAATTCG ATTGGTGTTC CGTTCGTGCT ATTAGAACTC TTCGGGAATC TGGTTTCAAG 
ACTGTCATGG TCAACTATAA CCCTGAGACT GTCAGTACCG ATTATGATGA AGCCGACAGA 
TTGTACTTTG AGAACATCAA CCTCGAAACC ATCCTCGACA TTTACGAATT GGAGTCTTCA 
AGTGGTGTTC TCATTGCTAT GGGAGGTCAA ACACCAAACA ACATTGCTCT CCCACTTCAC 
CGACAGAATG TCAAGATTCT TGGAACATCA CCAGAAATGA TTGATACTGC TGAGAATCGT 
TTCAAGTTCT CTCGTATGCT TGATGGCATC CATGTTGATC AACCAACATG GAAAGAGTTG 
ACTAGTTTCG AGGATGCTAA AACATTCTGT AACAAGGTCT CTTATCCAGT GCTCGTTAGA 
CCTTCATATG TGCTTTCAGG TGCAGCTATG AACACTGTTT ACTCCGAACC AGATCTTGAA 
GCTTATCTTG CTCAAGCTGT TGAAGTTTCA AGAGATCATC CAGTCGTTAT CACCAAATAT 
ATCGAAAATG CAAAGGAAAT TGAAATGGAC GCTGTTGCAA AGGATGGTGT TATGGTTGGT 
CACTTTATCT CTGAGCATGT TGAAAACGCC GGTGTACATT CTGGTGATGC TACTTTGATT 
TTACCACCTC AAGATCTCGA CCCTACCACT ATCAAGAGAA TTGAGGAAGC TACTCGCTTG 
ATTGGTAAAG CTCTTAACAT CACTGGTCCT TATAACATTC AATTCATCGC CAAGGACAAT 
GATATTAAGG TCATCGAATG TAATGTTCGT GCATCTAGAT CGACTCCATT CGTTTCAAAG 
GTCATGGGTG TTGATCTTAT TGAAATGGCT ACTAAAGCCA TTATGGGTGT ACCATTCATG 
CCATACCCAC CTACCGAATT ACCTGCAGGC TGCGTTGGTG TTAAAGTACC TCAATTCAGT 
TTCTCTCGAC TTTCTGGTGC TGATCCAGTT CTTGGTGTCG AGATGGCTTC TACTGGTGAA 
GTTGCTTGTT TCGGTCGTGA TAAGTATGAG GCTTACATCA AAGCATTGAT CTCTACTGGA 
TTCAAATTAC CAAAGCAAAA CATTCTCCTT TCTATTGGTT CATACAAGGA CAAGAAGGAA 
ATGCTTCCAT CTGTTGTCAA GCTTCAAAAG TTGGGTTACA AACTTTTCGC TACAGCCGGT 
ACCGCAGATT TCTTACAAGA ACATGATGTC CCAGTTCAGT ACCTCGAAAT TCTCGGAAAG 
GGTGAGGATG ATCAAAAATC GGAATATTCT TTGACTCAAC ATCTTGCCAA TAACATGATT 
GATCTTTATA TCAATCTTCC TTCCAGCAAC AGATATAGAA GACCTGCCAA CTACATGAGT 
AAGGGTTACC AAACTAGACG TATGGCTGTC GATTATCAAA CACCTTTGGT CACCAACGTC 
AAGAACGCCA AGATCCTTAT CGAAGCCATC GCAAGACATT ATGATATGGA AGTCAGTGGA 
TTCGATGCAC AAACTAGTCA CAGAACTGTT GTTCTTCCTG GTCTTATCAA CATTGCATCT 
TTCGTTCCTG GCATCACCAC TCCTGGCAGC CGTGATTTCC AAACTGTCAC CAAAGCATCA 
ATTGCATCTG GTTTCAGTAT GATTCGTGTT ATGCCTCTTG GTCTTGATGG ATCAGTCACT 
GATGCTAGAG CATTGAAGGT AGCTCAACTT AACAGCAAAC TTGGTTCATC TTGCGATTAC 
AATTTCTCTG TTGCCGCAAC TTCATCTAAC GCCGATCAAA TTAGTCAAGT CACTGGTGAA 
GTTGGCTCTC TCTTCATCCC ATTCAATCAC TTGTCCGATA ATATCAGCAA GGTAGCTGCT 
GTTACTGCTC ATTTTGAATC TTGGCCATCT TTCAAGCCAA TCATCACTGA TGCAAAGATG 
ACCGATCTTG CATCAATTCT ACTTCTCGCA AGTCTCCATA GCCGAAAGAT TCACATCACC 
AATGTCACCA CCAAGGATGA TATTGGATTG ATTGATTTGG CTAAGCAAAA AGGTCTTCAA 
GTCACTTGTG ATGTTTCAGT GTACGCCCTT TTCTTCAGCC AAGATGATTA CCCTGAAGCT 
TCTTTCCTTC CAACTGCCAA GGACCAAGAA GCATTATGGG AACACATGAA CATCATTGAT 
GTCTTTTCTA TTGGTAGCTT ACCATATCAA CTTGCAACTC TTGCAAAGGC AGAGGCAGAC 
GCCGCTGTTG GTATTGCTGA CACTTTGCCT CTACTGTTCT CAGCTGTTGC AGATGGTAGA 
CTCACAGTCG ATGATATTAA GGCTCGTCTC CATGACAATC CAAAGGAGAT TTTCGAAATT 
CACGATCAAG TCGGAGCTTC CGTAGAGGTT GAGATTGATA GACCACACAC TGTCAAGCCA 
GGAACAGTTT GGTCGCCACT TGTTGGACAG GTCACAAGAG GAGCTGTCCA AAGAGTCATC 
TTCCAAGATA AGACTGCTTG TCTAGATGGT TTACCAACTC AAGATGCTAT TGATGGTAAA 
GATATGTCAT CTCATCTTAT TGATTTACAT ACCACAGTAC CACAATCACC ACTTGTCAAG 
GCTAATCTTG GATCCCCAGC TATGGGACCA AGACCTGATA GCTCTCATGG ATTGAGAAAG 
TTCTCTTCAT TTGCTGGTGC ATCATCTTCA GCTATGCGAC CTGCAACTCG TTCAAGGTTC 
TTGGACGGTG CTGCCAGCCA AGCACCACAA CTTGGTTCAA CAGCTGAAGA TTTGGGTGCT 
CCATTATACC CTCAACCAAC AATATCACCA TCTCTACAAA CCCTCCTCGG CCAATCGCCA 
TTCAAGAATC AACACGTTCT TTCGGTCAAT CAATTCTCCC GTCAAGATCT TCATCTTCTT 
TTCTCTGTTG CTCAAGAAAT GAGATTGGGT GTTCAACGCG AAGGTGTTCT TTCTATTCTC 
AAGGGACGTC TTCTTTGCAC AATGTTCTAC GAACCATCTA CTCGTACATC TGCATCATTT 
GATGCTGCTA TGCAAAGATT GGGTGGACGA ACAGTTGCAA TCGCTACTTC TCATTCTTCC 
ACTGTCAAGG GCGAATCTCT TGCAGATACC ATCCGCACTC TTGGTTGTTA TGGAGATGCT 
ATTGTCCTTC GTCACCCTGA AGAGAGTAGT GCTTCAACTG CTGCCAAATT TTCTCCAGTT 
CCAATTATCA ACGGAGGTAA TGGAAGCAAA GAACACCCAA CTCAAGCATT CTTGGATTTG 
TTCACAATCA GAGAAGAATT GGGTACTGTT GGTGGTCTTA CCATTACCTT TACTGGTGAT 
CTTAGATATG GTCGTACCGT TCACTCTCTC GTCAAGCTTC TTCAACATTA TGATGTTCAC 
ATCCAGCTTG TCTCCCCACA AGCTTTATCC CTTCCACAAG AGATCTTGGC ACTTATCAAA 
TCTAAGCCTG GTCAATTACT TTCAGTTTCT ACTGAGTTGA CCCCCGAGAT CCTTGCTAGA 
TCCGATGTCC TTTACTGTAC TCGTGTACAA AAGGAGCGAT TTGCCGATTT GGAAGAGTAT 
GAGAGATTGA AGGATAGTTT CATCATTGAT AACAAGACAT TGAGAAATGC AAAGAGTTCA 
ATGATTGTTA TGCATCCATT GCCGAGAAAT GCCGAGATTG GAGAGGAAGT GGATTTCGAT 
CAAAGAGCTG CATACTTTAG ACAGATGAGA TATGGCCTTT ACTGTCGCAT GGCATTGTTG 
GCTTTGGTAT TAGCCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001553899.1
CDS	174..6914
Translation	MAELVFPPVAAPILNGTDEGLISLELKDGTIYQGYSFGAQKSVAGELVFQTGMVGYPESITDPSYRGQILVITFPLVGNYGVPSRETMDELLKDLPAHFEASQIHIAGLICASYAGEEFSHFLATSSLGAWLKEQGVPAIYGVDTRALTKRIREEGSMLGRMLLQKNIPASGNDNISALSTKDWRSSFEAIDWVDPNAKNLVADVSIREPKLYKPDPSNALKHPSGRPIRVLCLDVGLKYNQLRCLVKRGVEVLVCPWDYDFPALAGKDYDGLFISNGPGDPAMMDKTVKHIQSALKENRTPIFGICLGHQLLARAAGAQTEKMKFGNRGHNIPCTNMLTGKCHITSQNHGFAVQTSSLPSEWEELFVNANDGSNEGIRHVNRPYFSVQFHPESTPGPRDTEFLFDVFINTIQKTLDDEKLLSAPVVFPGGEKAENEKLTPKVNVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVTADFVRKVIKHEKPDAIYVTFGGQTALSVGIQLKDEFEELGVRVLGTPISTVITTEDRELFARSMESIGEKCAKSASANNIDEAMHVVKDIGFPVIVRAAYALGGLGSGFADNEEELLELCNKAFAASPQVLIERSMKGWKEIEYEVVRDCRDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPDSKEYCIIEVNARLSRSSALASKATGYPLAFIAAKLGLNIPLNEIKNSVTQQTCACFEPSLDYVVVKMPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRAIDFHNLGFSATKALMSIDDELQTPSDQRLFAIANAMQSGYSVNKIWEMTKIDKWFLNKLKGLNDFSKHMSTFNTSTISYDQIRQAKQLGFCDRQLAKFWDSTELAVRLLRTEAGIVPVVKQIDTVAAEFEAHTNYLYMTYNGSESDISFNDHGVMVLGSGVYRIGSSVEFDWCSVRAIRTLRESGFKTVMVNYNPETVSTDYDEADRLYFENINLETILDIYELESSSGVLIAMGGQTPNNIALPLHRQNVKILGTSPEMIDTAENRFKFSRMLDGIHVDQPTWKELTSFEDAKTFCNKVSYPVLVRPSYVLSGAAMNTVYSEPDLEAYLAQAVEVSRDHPVVITKYIENAKEIEMDAVAKDGVMVGHFISEHVENAGVHSGDATLILPPQDLDPTTIKRIEEATRLIGKALNITGPYNIQFIAKDNDIKVIECNVRASRSTPFVSKVMGVDLIEMATKAIMGVPFMPYPPTELPAGCVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRDKYEAYIKALISTGFKLPKQNILLSIGSYKDKKEMLPSVVKLQKLGYKLFATAGTADFLQEHDVPVQYLEILGKGEDDQKSEYSLTQHLANNMIDLYINLPSSNRYRRPANYMSKGYQTRRMAVDYQTPLVTNVKNAKILIEAIARHYDMEVSGFDAQTSHRTVVLPGLINIASFVPGITTPGSRDFQTVTKASIASGFSMIRVMPLGLDGSVTDARALKVAQLNSKLGSSCDYNFSVAATSSNADQISQVTGEVGSLFIPFNHLSDNISKVAAVTAHFESWPSFKPIITDAKMTDLASILLLASLHSRKIHITNVTTKDDIGLIDLAKQKGLQVTCDVSVYALFFSQDDYPEASFLPTAKDQEALWEHMNIIDVFSIGSLPYQLATLAKAEADAAVGIADTLPLLFSAVADGRLTVDDIKARLHDNPKEIFEIHDQVGASVEVEIDRPHTVKPGTVWSPLVGQVTRGAVQRVIFQDKTACLDGLPTQDAIDGKDMSSHLIDLHTTVPQSPLVKANLGSPAMGPRPDSSHGLRKFSSFAGASSSAMRPATRSRFLDGAASQAPQLGSTAEDLGAPLYPQPTISPSLQTLLGQSPFKNQHVLSVNQFSRQDLHLLFSVAQEMRLGVQREGVLSILKGRLLCTMFYEPSTRTSASFDAAMQRLGGRTVAIATSHSSTVKGESLADTIRTLGCYGDAIVLRHPEESSASTAAKFSPVPIINGGNGSKEHPTQAFLDLFTIREELGTVGGLTITFTGDLRYGRTVHSLVKLLQHYDVHIQLVSPQALSLPQEILALIKSKPGQLLSVSTELTPEILARSDVLYCTRVQKERFADLEEYERLKDSFIIDNKTLRNAKSSMIVMHPLPRNAEIGEEVDFDQRAAYFRQMRYGLYCRMALLALVLAP

Target ORF information:

RefSeq Version	XM_001553849.2
Organism	Botrytis cinerea B05.10
Definition	Botrytis cinerea B05.10 hypothetical protein (BCIN_12g04860), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001553849.2

ORF Insert Sequence:

ATGGCAGAAT TAGTTTTCCC TCCCGTTGCC GCCCCAATCC TCAATGGCAC TGATGAAGGA 
CTCATCAGTT TGGAATTGAA GGATGGCACA ATTTATCAAG GATATAGTTT TGGTGCGCAG 
AAGAGTGTCG CGGGAGAATT GGTCTTCCAG ACAGGTATGG TGGGGTACCC AGAATCCATC 
ACTGACCCTT CATACCGTGG ACAAATCTTG GTCATTACGT TCCCTTTGGT GGGCAATTAT 
GGTGTACCAT CTCGAGAAAC CATGGATGAA CTCCTCAAGG ATCTGCCAGC GCATTTCGAA 
GCATCACAGA TCCACATCGC AGGTTTGATT TGCGCTTCAT ACGCAGGCGA AGAGTTTTCT 
CATTTCCTCG CAACCTCTTC TTTGGGAGCA TGGTTGAAAG AGCAAGGTGT CCCAGCAATC 
TATGGTGTTG ACACCAGAGC TTTAACCAAG AGAATACGAG AAGAAGGTAG TATGCTTGGA 
CGAATGTTGT TGCAAAAGAA CATCCCTGCC AGTGGAAACG ACAACATCTC TGCTTTATCT 
ACAAAGGATT GGAGATCATC ATTCGAAGCA ATTGATTGGG TCGACCCCAA TGCCAAGAAC 
CTTGTCGCAG ATGTATCAAT TCGCGAGCCA AAATTATACA AACCTGATCC TTCGAATGCT 
TTGAAACATC CTTCTGGTAG ACCTATACGT GTGTTGTGTT TGGACGTTGG TTTGAAATAC 
AACCAGCTGC GTTGTCTTGT AAAGCGTGGT GTGGAAGTTC TCGTCTGCCC ATGGGATTAT 
GATTTCCCTG CTCTCGCCGG CAAGGATTAT GACGGTCTAT TCATCTCCAA CGGTCCTGGT 
GATCCAGCCA TGATGGATAA AACTGTCAAG CACATTCAAT CTGCCTTGAA GGAGAACCGT 
ACACCAATTT TCGGTATCTG TCTTGGACAT CAACTTCTCG CTAGGGCTGC TGGAGCTCAA 
ACCGAAAAGA TGAAGTTCGG TAACCGTGGA CATAACATTC CATGCACAAA CATGTTGACC 
GGAAAATGTC ATATTACTTC CCAAAACCAC GGTTTCGCTG TCCAAACTAG CTCCTTGCCT 
TCAGAATGGG AGGAACTTTT CGTTAATGCC AACGATGGAA GTAACGAGGG TATCAGACAT 
GTCAACAGGC CATACTTCAG TGTTCAATTC CATCCAGAAA GTACTCCTGG ACCAAGGGAT 
ACTGAATTCC TCTTTGATGT CTTCATCAAC ACTATCCAAA AGACTCTCGA TGACGAAAAA 
CTTCTTTCCG CACCAGTTGT CTTCCCAGGT GGTGAGAAAG CTGAGAATGA GAAGCTTACG 
CCTAAGGTCA ACGTCAAGAA AGTCCTTGTT TTGGGTAGTG GTGGTCTTAG TATCGGTCAA 
GCAGGCGAAT TCGATTACTC TGGAAGTCAA GCTATCAAAG CTCTTAAAGA GGAAGGAATC 
TATACCATCT TGATCAACCC TAACATCGCT ACAATTCAAA CTTCCAAAGG ATTGGCTGAT 
AAGGTCTATT TCCTTCCCGT CACAGCTGAT TTTGTTCGCA AAGTCATCAA GCACGAGAAG 
CCTGATGCAA TTTACGTCAC ATTTGGTGGC CAAACTGCTT TATCTGTTGG TATTCAACTC 
AAGGATGAAT TCGAAGAGCT CGGAGTTCGC GTCCTCGGTA CCCCAATTTC CACTGTCATC 
ACTACTGAAG ATCGAGAGTT GTTCGCTCGC AGTATGGAGT CGATTGGTGA GAAATGCGCC 
AAATCCGCTT CAGCGAACAA CATCGATGAA GCTATGCATG TCGTGAAGGA CATTGGATTC 
CCAGTCATCG TTCGCGCTGC ATATGCTTTG GGAGGTCTTG GAAGTGGTTT TGCAGACAAT 
GAGGAAGAAT TACTTGAACT CTGCAACAAA GCTTTCGCCG CCAGTCCTCA AGTTTTGATC 
GAGCGAAGTA TGAAGGGATG GAAGGAAATT GAGTACGAGG TTGTCCGTGA TTGTCGTGAC 
AACTGTATCA CAGTTTGTAA CATGGAGAAC TTCGATCCTC TGGGTATTCA CACTGGAGAC 
TCCATTGTTG TTGCTCCTTC TCAAACATTA TCCGATGAGG ATTACAACAT GTTGAGAACC 
ACAGCTGTTA ACGTCATTCG CCATCTCGGA GTTGTTGGAG AATGTAACAT TCAATACGCA 
CTCAACCCTG ACTCGAAGGA ATACTGTATT ATCGAGGTCA ACGCTAGATT GTCCAGATCT 
TCAGCCCTCG CATCCAAGGC TACTGGATAT CCATTGGCTT TCATCGCCGC AAAGCTCGGT 
CTTAACATCC CACTCAACGA AATCAAGAAC TCAGTCACTC AACAGACCTG CGCTTGTTTC 
GAACCATCTT TGGATTATGT TGTTGTTAAG ATGCCACGTT GGGATTTGAA GAAGTTCACT 
CGTGTTTCTA CACAACTTGG TTCATCAATG AAGAGTGTCG GTGAAGTTAT GAGTATCGGA 
AGAACTTTCG AAGAAGCTAT TCAGAAAGCA ATCCGTGCTA TTGATTTCCA TAACTTGGGT 
TTCAGTGCAA CAAAGGCATT AATGTCAATT GATGATGAAC TTCAAACGCC TTCCGATCAA 
CGTCTCTTTG CAATCGCTAA TGCCATGCAA TCTGGTTACT CTGTTAACAA GATTTGGGAA 
ATGACAAAGA TTGACAAATG GTTCTTGAAC AAGCTCAAGG GATTGAATGA CTTCAGCAAG 
CACATGTCAA CCTTCAACAC TAGCACCATC TCATATGACC AAATCAGACA AGCCAAACAA 
CTTGGATTCT GCGATAGACA ATTGGCTAAA TTCTGGGATT CTACAGAACT TGCTGTTAGA 
TTGTTGAGAA CCGAAGCTGG TATTGTCCCA GTTGTTAAGC AAATTGATAC CGTTGCTGCC 
GAGTTCGAGG CCCACACCAA TTATCTTTAC ATGACCTACA ACGGATCTGA GAGCGATATC 
TCTTTCAATG ACCACGGTGT CATGGTCCTC GGTTCTGGAG TTTACAGAAT TGGTTCTTCC 
GTCGAATTCG ATTGGTGTTC CGTTCGTGCT ATTAGAACTC TTCGGGAATC TGGTTTCAAG 
ACTGTCATGG TCAACTATAA CCCTGAGACT GTCAGTACCG ATTATGATGA AGCCGACAGA 
TTGTACTTTG AGAACATCAA CCTCGAAACC ATCCTCGACA TTTACGAATT GGAGTCTTCA 
AGTGGTGTTC TCATTGCTAT GGGAGGTCAA ACACCAAACA ACATTGCTCT CCCACTTCAC 
CGACAGAATG TCAAGATTCT TGGAACATCA CCAGAAATGA TTGATACTGC TGAGAATCGT 
TTCAAGTTCT CTCGTATGCT TGATGGCATC CATGTTGATC AACCAACATG GAAAGAGTTG 
ACTAGTTTCG AGGATGCTAA AACATTCTGT AACAAGGTCT CTTATCCAGT GCTCGTTAGA 
CCTTCATATG TGCTTTCAGG TGCAGCTATG AACACTGTTT ACTCCGAACC AGATCTTGAA 
GCTTATCTTG CTCAAGCTGT TGAAGTTTCA AGAGATCATC CAGTCGTTAT CACCAAATAT 
ATCGAAAATG CAAAGGAAAT TGAAATGGAC GCTGTTGCAA AGGATGGTGT TATGGTTGGT 
CACTTTATCT CTGAGCATGT TGAAAACGCC GGTGTACATT CTGGTGATGC TACTTTGATT 
TTACCACCTC AAGATCTCGA CCCTACCACT ATCAAGAGAA TTGAGGAAGC TACTCGCTTG 
ATTGGTAAAG CTCTTAACAT CACTGGTCCT TATAACATTC AATTCATCGC CAAGGACAAT 
GATATTAAGG TCATCGAATG TAATGTTCGT GCATCTAGAT CGACTCCATT CGTTTCAAAG 
GTCATGGGTG TTGATCTTAT TGAAATGGCT ACTAAAGCCA TTATGGGTGT ACCATTCATG 
CCATACCCAC CTACCGAATT ACCTGCAGGC TGCGTTGGTG TTAAAGTACC TCAATTCAGT 
TTCTCTCGAC TTTCTGGTGC TGATCCAGTT CTTGGTGTCG AGATGGCTTC TACTGGTGAA 
GTTGCTTGTT TCGGTCGTGA TAAGTATGAG GCTTACATCA AAGCATTGAT CTCTACTGGA 
TTCAAATTAC CAAAGCAAAA CATTCTCCTT TCTATTGGTT CATACAAGGA CAAGAAGGAA 
ATGCTTCCAT CTGTTGTCAA GCTTCAAAAG TTGGGTTACA AACTTTTCGC TACAGCCGGT 
ACCGCAGATT TCTTACAAGA ACATGATGTC CCAGTTCAGT ACCTCGAAAT TCTCGGAAAG 
GGTGAGGATG ATCAAAAATC GGAATATTCT TTGACTCAAC ATCTTGCCAA TAACATGATT 
GATCTTTATA TCAATCTTCC TTCCAGCAAC AGATATAGAA GACCTGCCAA CTACATGAGT 
AAGGGTTACC AAACTAGACG TATGGCTGTC GATTATCAAA CACCTTTGGT CACCAACGTC 
AAGAACGCCA AGATCCTTAT CGAAGCCATC GCAAGACATT ATGATATGGA AGTCAGTGGA 
TTCGATGCAC AAACTAGTCA CAGAACTGTT GTTCTTCCTG GTCTTATCAA CATTGCATCT 
TTCGTTCCTG GCATCACCAC TCCTGGCAGC CGTGATTTCC AAACTGTCAC CAAAGCATCA 
ATTGCATCTG GTTTCAGTAT GATTCGTGTT ATGCCTCTTG GTCTTGATGG ATCAGTCACT 
GATGCTAGAG CATTGAAGGT AGCTCAACTT AACAGCAAAC TTGGTTCATC TTGCGATTAC 
AATTTCTCTG TTGCCGCAAC TTCATCTAAC GCCGATCAAA TTAGTCAAGT CACTGGTGAA 
GTTGGCTCTC TCTTCATCCC ATTCAATCAC TTGTCCGATA ATATCAGCAA GGTAGCTGCT 
GTTACTGCTC ATTTTGAATC TTGGCCATCT TTCAAGCCAA TCATCACTGA TGCAAAGATG 
ACCGATCTTG CATCAATTCT ACTTCTCGCA AGTCTCCATA GCCGAAAGAT TCACATCACC 
AATGTCACCA CCAAGGATGA TATTGGATTG ATTGATTTGG CTAAGCAAAA AGGTCTTCAA 
GTCACTTGTG ATGTTTCAGT GTACGCCCTT TTCTTCAGCC AAGATGATTA CCCTGAAGCT 
TCTTTCCTTC CAACTGCCAA GGACCAAGAA GCATTATGGG AACACATGAA CATCATTGAT 
GTCTTTTCTA TTGGTAGCTT ACCATATCAA CTTGCAACTC TTGCAAAGGC AGAGGCAGAC 
GCCGCTGTTG GTATTGCTGA CACTTTGCCT CTACTGTTCT CAGCTGTTGC AGATGGTAGA 
CTCACAGTCG ATGATATTAA GGCTCGTCTC CATGACAATC CAAAGGAGAT TTTCGAAATT 
CACGATCAAG TCGGAGCTTC CGTAGAGGTT GAGATTGATA GACCACACAC TGTCAAGCCA 
GGAACAGTTT GGTCGCCACT TGTTGGACAG GTCACAAGAG GAGCTGTCCA AAGAGTCATC 
TTCCAAGATA AGACTGCTTG TCTAGATGGT TTACCAACTC AAGATGCTAT TGATGGTAAA 
GATATGTCAT CTCATCTTAT TGATTTACAT ACCACAGTAC CACAATCACC ACTTGTCAAG 
GCTAATCTTG GATCCCCAGC TATGGGACCA AGACCTGATA GCTCTCATGG ATTGAGAAAG 
TTCTCTTCAT TTGCTGGTGC ATCATCTTCA GCTATGCGAC CTGCAACTCG TTCAAGGTTC 
TTGGACGGTG CTGCCAGCCA AGCACCACAA CTTGGTTCAA CAGCTGAAGA TTTGGGTGCT 
CCATTATACC CTCAACCAAC AATATCACCA TCTCTACAAA CCCTCCTCGG CCAATCGCCA 
TTCAAGAATC AACACGTTCT TTCGGTCAAT CAATTCTCCC GTCAAGATCT TCATCTTCTT 
TTCTCTGTTG CTCAAGAAAT GAGATTGGGT GTTCAACGCG AAGGTGTTCT TTCTATTCTC 
AAGGGACGTC TTCTTTGCAC AATGTTCTAC GAACCATCTA CTCGTACATC TGCATCATTT 
GATGCTGCTA TGCAAAGATT GGGTGGACGA ACAGTTGCAA TCGCTACTTC TCATTCTTCC 
ACTGTCAAGG GCGAATCTCT TGCAGATACC ATCCGCACTC TTGGTTGTTA TGGAGATGCT 
ATTGTCCTTC GTCACCCTGA AGAGAGTAGT GCTTCAACTG CTGCCAAATT TTCTCCAGTT 
CCAATTATCA ACGGAGGTAA TGGAAGCAAA GAACACCCAA CTCAAGCATT CTTGGATTTG 
TTCACAATCA GAGAAGAATT GGGTACTGTT GGTGGTCTTA CCATTACCTT TACTGGTGAT 
CTTAGATATG GTCGTACCGT TCACTCTCTC GTCAAGCTTC TTCAACATTA TGATGTTCAC 
ATCCAGCTTG TCTCCCCACA AGCTTTATCC CTTCCACAAG AGATCTTGGC ACTTATCAAA 
TCTAAGCCTG GTCAATTACT TTCAGTTTCT ACTGAGTTGA CCCCCGAGAT CCTTGCTAGA 
TCCGATGTCC TTTACTGTAC TCGTGTACAA AAGGAGCGAT TTGCCGATTT GGAAGAGTAT 
GAGAGATTGA AGGATAGTTT CATCATTGAT AACAAGACAT TGAGAAATGC AAAGAGTTCA 
ATGATTGTTA TGCATCCATT GCCGAGAAAT GCCGAGATTG GAGAGGAAGT GGATTTCGAT 
CAAAGAGCTG CATACTTTAG ACAGATGAGA TATGGCCTTT ACTGTCGCAT GGCATTGTTG 
GCTTTGGTAT TAGCCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Molecular plant pathology18(1)75-89(2017 01)
Van Kan JA,Stassen JH,Mosbach A,Van Der Lee TA,Faino L,Farmer AD,Papasotiriou DG,Zhou S,Seidl MF,Cottam E,Edel D,Hahn M,Schwartz DC,Dietrich RA,Widdison S,Scalliet G

Genome update of Botrytis cinerea strains B05.10 and T4.
Eukaryotic cell11(11)1413-4(2012 Nov)
Staats M,van Kan JA

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
PLoS genetics7(8)e1002230(2011 Aug)
Amselem J,Cuomo CA,van Kan JA,Viaud M,Benito EP,Couloux A,Coutinho PM,de Vries RP,Dyer PS,Fillinger S,Fournier E,Gout L,Hahn M,Kohn L,Lapalu N,Plummer KM,Pradier JM,Quévillon E,Sharon A,Simon A,ten Have A,Tudzynski B,Tudzynski P,Wincker P,Andrew M,Anthouard V,Beever RE,Beffa R,Benoit I,Bouzid O,Brault B,Chen Z,Choquer M,Collémare J,Cotton P,Danchin EG,Da Silva C,Gautier A,Giraud C,Giraud T,Gonzalez C,Grossetete S,Güldener U,Henrissat B,Howlett BJ,Kodira C,Kretschmer M,Lappartient A,Leroch M,Levis C,Mauceli E,Neuvéglise C,Oeser B,Pearson M,Poulain J,Poussereau N,Quesneville H,Rascle C,Schumacher J,Ségurens B,Sexton A,Silva E,Sirven C,Soanes DM,Talbot NJ,Templeton M,Yandava C,Yarden O,Zeng Q,Rollins JA,Lebrun MH,Dickman M

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BCIN_12g04860 cDNA ORF clone, Botrytis cinerea B05.10

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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