MYOF gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024985589.1	XP_024841357.1	myoferlin isoform X3
NM_001193212.1	NP_001180141.1	myoferlin
NM_001193212.2	NP_001180141.1	myoferlin
XM_010819886.1	XP_010818188.1	myoferlin isoform X5
XM_005225523.3	XP_005225580.1	myoferlin isoform X6
XM_024985591.1	XP_024841359.1	myoferlin isoform X4
XM_010819883.2	XP_010818185.1	myoferlin isoform X2
XM_010819884.2	XP_010818186.1	myoferlin isoform X3
XM_010819885.2	XP_010818187.1	myoferlin isoform X4
XM_005225521.3	XP_005225578.1	myoferlin isoform X1
XM_005225523.4	XP_005225580.2	myoferlin isoform X5
NM_001193212.3	NP_001180141.2	myoferlin
XM_024985587.1	XP_024841355.1	myoferlin isoform X1
XM_024985588.1	XP_024841356.1	myoferlin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230835	XM_005225523.4 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa33557	XM_005225523.3 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa48677	XM_010819886.1 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa216187	NM_001193212.2 Latest version!	Bos taurus myoferlin (MYOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa239152	NM_001193212.3 Latest version!	Bos taurus myoferlin (MYOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa40769	NM_001193212.1 Latest version!	Bos taurus myoferlin (MYOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa230831	XM_024985587.1 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa230832	XM_024985588.1 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa230833	XM_024985589.1 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa230834	XM_024985591.1 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa33555	XM_005225521.3 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa48674	XM_010819883.2 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa48675	XM_010819884.2 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa48676	XM_010819885.2 Latest version!	Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

MYOF ( XM_005225523.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225580.2 Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa230835
Clone ID Related Accession (Same CDS sequence)	XM_005225523.4
Accession Version	XM_005225523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225523.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225580.2
CDS	251..5443
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFGV

Target ORF information:

RefSeq Version	XM_005225523.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225523.4

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_005225523.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225580.1 Bos taurus myoferlin (MYOF), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa33557
Clone ID Related Accession (Same CDS sequence)	XM_005225523.3
Accession Version	XM_005225523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225580.1
CDS	248..5440
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFGV

Target ORF information:

RefSeq Version	XM_005225523.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225523.3

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_010819886.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818188.1 Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48677
Clone ID Related Accession (Same CDS sequence)	XM_010819886.1
Accession Version	XM_010819886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACC AGAGCAGATC ACTGCCCTAC AAACTGGTCT CCCTGCTAAA CGAAAGGGGA 
CAAGAGACTG GGGCCACAAT TGACCTGGTG ATTGGCTATC ATCCACCTTC AGCTCCACAT 
CCAAATGACC CCGGTGGGAC CAGCGTGCCA GGCACAGGAG AAGAAGAGGA AGATGGAGGT 
GATGAGGACA TGTTGGACAG TACGGTCAGG GGCCCAGGGC CCAGGGGGCC AGGAGGGACC 
GTGCCGGAAG CTCACCTTGC CCGGAGGCTC ACGAAAGCGA AGAGCAGCCG CCGCATGCTG 
TCTAACAAGC CACAGGACTT CCAGATCCGG GTCCGAGTGA TCGAGGGCCG GCAGTTGAGT 
GGCAACAACA TCAAGCCTGT GGTCAAAGTC CACATCTGTG GCCAGACGCA TCGAACAAGA 
ATCAAGCGAG GGAACAATCC ATTTTTTGAT GAGTTATTTT TCTACAATGT CCACCTGACT 
CCTTCTGAAC TGATGGATGA GATCATCAGC ATTCGGGTTT ACAATTCCCA TTCTCTGCGG 
GCAGATTCTC TGATGGGGGA GTTTAAGATC GATGTTGGAT TTGTTTATGA TGAACCTGGT 
CATGCTGTCA TGAGGAAATG GCTTCTCCTC AATGACCCTG AAGATACCAG CTCGGGTGCT 
AAAGGCTACC TGAAAGTCAG CATGTTCGTC CTGGGAACTG GAGACGAGCC TCCTCCTGAG 
AAACAAGATC GTGATAATGA CAGTGATGAC GTGGAAAGTA ACTTGTTACT GCCTGCTGGC 
ATTGCCCTGC GGTGGGTGAC CTTCCTGCTG AAGATCTACA GAGCGGAGGA CATCCCCCAG 
ATGGATGATG CCTTCTCACA GACAGTGAAG GAAATATTTG GAGGCGTCGC TGATAAGAAG 
AACCTAGTGG ACCCTTTTGT AGAAGTTTCC TTTGCCGGAA AAAAGGTTTG CACGAACATA 
ATTGAGAAAA ATGCAAACCC TGAGTGGAAT CAAGTCGTTA ATCTTCAGAT CAAGTTTCCT 
TCAGTGTGTG AAAAAATAAA ACTGACAATA TATGACTGGG ACCGTCTTAC CAAAAATGAT 
GTTGTTGGAA CAACGTATCT GCACCTCTCT AAAATTGCTG CCTCTGGTGG GGAAGTGGAA 
GATTTCTCAT CCTCGGGATC TGGGGCTGCA TCGTATACAG CGAATACAGG AGAAACAGAG 
GTGGGCTTTG TTCCAACTTT TGGACCTTGT TATCTGAACC TTTATGGAAG CCCCCGAGAG 
TACACGGGAT TCCCAGATCC CTATGATGAG CTAAATTCTG GAAAGGGGGA AGGAGTTGCC 
TACAGAGGCA GAATCTTGGT TGAATTAAGC ACTTTACTTG AAAAGACACC ACCAGATAAA 
AAGCTTGAGT CCATTTCGCA TGATGACCTG CTGGTTGTTG AGAAATATCA GCGAAGGCGG 
AAGTACAGCC TGTCTGCTGT GTTCCATTCA GCCACCATGT TGCAAGACAT TGGCGAGGCC 
ATTCAGTTTG AAGTCAGCAT CGGGAACTAC GGCAACAAGT TTGACACGAC TTGTAAGCCT 
CTGGCATCAA CCACTCAGTA TAGCCGTGCT GTATTTGATG GAAACTACTA TTACTACTTG 
CCTTGGGCCC ACACGAAGCC AGTTGTTACA CTGACTTCCT ACTGGGAGGA TATTAGTCAT 
CGCCTGGATG CTGTGAACAC GCTCCTAGTC ATGGCAGAAA GGCTGCAATC AAACATAGAG 
GCTCTAAAAG CAGGGATACA AGGTAAAATT CCTTCAAACG AACTGGCAAA AATGTGGCAT 
AAGCTCATGG ATGAAGTTAT CGAAGACACA AGATACACGT TACCTCTCAC AGAAGGAAAA 
GCTAATGTCA CGGTTCTCGA TACTCAGATC CGAAAGTTGC GATCCAGAGC TCTCTCTCAG 
ATACATGAGG CAGCTGTGAG GATGCGGTCT GAGGTCCCAG ACGTCAAGTC CACATTGGTG 
GAAATTGAGG ACTGGCTCGA TAAATTAATG CAGCTGACTG AGGAGCCTCA GAACAGCATG 
CCTGACATCA TCATCTGGAT GATCCGGGGA GAGAAGAGAT TGGCCTACGC ACGCATTCCC 
GCGCATCAGG TTTTATACTC CACCAGCGGC GGGGATGCGT CTGGGAAGTA CTGTGGGAAA 
ACCCAGACCA TCTTTCTGAA GTATCCACAA GAAAAAAACA ACGGACCAAA GGTGCCCGTG 
GAGTTGCGTG TGAACATCTG GCTGGGCTTA AGTGCTGTTG AGAAGAAGTT CAATAGCTTC 
GCAGAAGGAA CATTCACCGT CTTTGCTGAA ATGTATGAAA ATCAAGCTCT CATGTTTGGA 
AAATGGGGCA CTTCCGGATT AGTGGGTCGT CATAAATTTT CCGATGTCAC AGGAAAAATA 
AAACTCAAGA GGGAATTCTT TTTGCCTCCA AAAGGCTGGG AATGGGAAGG AGACTGGATA 
GTTGATCCTG AAAGAAGCTT GCTGACCGAG GCGGATGCGG GTCACACAGA GTTCACAGAT 
GAAGTGTACC AGAACGAGAG TCGCTACCCC GGGGGCGAGT GGAAGCCGGC GGAGGACACC 
TACACCGATG CGAACGGCGA TAAAGCAGCA TCACCAAGCG AGCTCACTTG CCCTCCAGGC 
TGGGAATGGG AGGATGATGC ATGGGTCTAT GACATTAATC GTGCAGTGGA TGAGAGAGGC 
TGGGAATATG GAATTACCAT TCCTCCTGAT AATAAGCCCA AATCCTGGGT TGCAGCAGAA 
AAAATGTATC ATACTCATAG ACGCCGAAGG CTGGTCCGAA AACGCAAGAA AGATTTAACA 
CAATCCACTT CGAGTACCGC AAGGGCCATG GCAGAATTTG AAGACCGGGA GGGCTGGGAA 
TACGCTTCTC TAATTGGCTG GAAATTTCAC TGGAAACAGC GTAGTTCAGA TACGTTTCGC 
CGCAGACGCT GGAGGAGAAA AATGGCTCCT TCAGAAACAC ATGGTGCAGC TGCCATCTTT 
AAACTTGAAG GGGCCCTTGG CGCAGACACT ACGGACGATG GAGAGGAAAA GAGCGCAGAA 
AAGCAGAAGC ACAGCGCCAC CACTGTATTT GGAGCAAACA CTCCCATTGT TTCCTGTAAT 
TTTGACAGAG TCTACATTTA CCACCTGCGC TGCTACATCT ATCAAGCCAG GAACCTCATG 
GCTCTAGACA AGGATAGCTT TTCAGATCCA TATGCTCATG TCTCCTTCCT TCATCGAAGT 
AAAACCACCG AGATCATCCA CTCAACTCTG AATCCGACAT GGGACCAAAC GATTATATTC 
GATGAAATTG AAATTTATGG GGAACCCCAA ACCATCCTAC AGAACCCACC CAAGGTTATC 
ATCGAACTTT TTGACAATGA CCAAGTGGGC AAAGATGAAT TTTTAGGACG AAGCATGTGC 
TCTCCTCTGG TGAAACTAGA CTCAGAAATG GACATCACTC CCAAACTTCT CTGGTACCCA 
GTGATGAATG GAGACAAAAC CTGTGGGGAT GTCCTTGTAA CTGCAGAGTT GATTCTGAGG 
CACAAGGATG GCTCCAACCT TCCCATTCTT CCCTCGCAAA GGGCACCAAA TCTGTACATG 
GTCCCCCAGG GCATCAGGCC TGTGGTTCAG CTCACTGCTA TTGAGATTCT AGCTTGGGGC 
TTAAGAAATA TGAAGAATTA CCAGATGGCT TCTGTCACCT CCCCCAGTCT CATTGTGGAG 
TGTGGAGGGG AAAGGGTCGA GTCAGTGGTG ATCAAAAACC TGAAGAAGAC ACCCAACTTC 
CCAAGTTCTG TTCTCTTCAT GAGAGTGTTC TTGCCCAAGG AGGAACTGTA CATGCCTCCA 
CTGGTGATCA AGGTCATCGA CCACCGGCAG TTCGGGCGCA AGCCCGTTGT GGGCCAGTGC 
ACCATTGATC GCCTGGACCG CTTTCGCTGT GACCCTTATG CAGGAAAGGA GGACATCGTG 
CCACAGCTAA AAGCCTCCTT TTTGTCTACC CCACCCCGCC GGGACATTGT TATTGAAATA 
GAGGACACCA AGCCATTACT AGCTTCAAAG TGCTTAAGCA GTATGTCAAC AGCACTCAGC 
AAAATGGCTT CTCCAAGGAC AGTGCATCTA ACAGAAAAGG AGGAAGAAAT TGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CACAGGGGAA CATGAAAAAT GTGGACAATA TATTCAGAAA 
GGTTATTCCA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAGAGGA ATTCGAGGGC 
CTGACGGACT TCTCAGATAC TTTCAAGCTG TTTCGAGGCA AATCAGATGA GAATGAAGAC 
CCTTCTGTGG TAGGGGAGTT TAAGGGCTCC TTCCGAATCT ACCCACTGCC GGATGACCCC 
AGCGTGCCGG CCCCTCCCCG GCAGTTTCGG GAGTTACCTG ACAGCGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTAA TTGAAGACCG AGACAACTAC 
ATCCCCAACA CTCTCAACCC GGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAAAAGG ACCTGAAAAT TTCTGTCTAC GATTATGACA CCTTTACCCG TGATGAAAAA 
GTAGGAGAAA CGATCATTGA CCTAGAAAAC CGATTCCTTT CCCGGTTTGG GTCCCACTGT 
GGCATTCCTG AGCAGTACTG CGTTTCTGGA GTAAACACCT GGCGAGATCA ATTGAAACCA 
ACACAGTTGC TTCAAAATGT GGCCAGATTC AAAGGCTTCC CACCGCCCGT TCTTTCTGAA 
GATGGAAGTA GCATCAGATA TGGAGGAAAA GACTACGGCC TAGATGAATT TGAAGCAAAC 
AAAATCCTGC ACCAGCACCT TGGGCCCCCT GAGGAGCGCC TTGCCCTCTA CATCCTCAGG 
ACTCAGGGGC TGGTTCCTGA ACACGTGGAA ACGAGGACTT TGCACAGCAG CTTCCAGCCT 
AACATCTCCC AGGGAAGACT TCAGATGTGG GTGGATGTTT TCCCCAAGAA TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATT 
ATCTGGAACA CCAAGGATGT TGTCCTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGC 
GACATCTATG TGAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGGT CCTTGGATGG CGTAGGGAAC TTTAACTGGA GATTCGTCTT CCCTTTTGAC 
TACCTCCCAG CTGAACAGCT CTGTCTCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACTGAAT TTCGGGTCCC ACCCAGACTG GTCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTGGAA CTTGATTTGC ATCATACCAT CATTCCAGCA 
AAGTCACCAG AGAAATGCAG TTTGAACATG ATTCCAGACC TCAAAGCCAT GAACCCCCTG 
AAAGTGAAAA CTGCCTCTCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCTTGCTAC 
ACAGAGAAAG ATGGCTCCCG TGTGATGGCT GGGAAAGTGG AGATGACGCT GGAAGTCCTC 
AATGAGAAGG AGGCCGACGA GAGGCCAGCT GGGAAGGGAC GGGATGAACC CAACATGAAC 
CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCGTGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGA TTTAAGTGGG TCATCATTGC CCTGCTGATC 
CTGCTGATCC TGCTGTTCTT TGTGGCTGTG TTTCTGTATT CCTTACCGAA CTATTTGTCA 
ATGAAGATTG TGAAACCAAA TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818188.1
CDS	228..6194
Translation	MGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_010819886.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819886.1

ORF Insert Sequence:

ATGGGTGACC AGAGCAGATC ACTGCCCTAC AAACTGGTCT CCCTGCTAAA CGAAAGGGGA 
CAAGAGACTG GGGCCACAAT TGACCTGGTG ATTGGCTATC ATCCACCTTC AGCTCCACAT 
CCAAATGACC CCGGTGGGAC CAGCGTGCCA GGCACAGGAG AAGAAGAGGA AGATGGAGGT 
GATGAGGACA TGTTGGACAG TACGGTCAGG GGCCCAGGGC CCAGGGGGCC AGGAGGGACC 
GTGCCGGAAG CTCACCTTGC CCGGAGGCTC ACGAAAGCGA AGAGCAGCCG CCGCATGCTG 
TCTAACAAGC CACAGGACTT CCAGATCCGG GTCCGAGTGA TCGAGGGCCG GCAGTTGAGT 
GGCAACAACA TCAAGCCTGT GGTCAAAGTC CACATCTGTG GCCAGACGCA TCGAACAAGA 
ATCAAGCGAG GGAACAATCC ATTTTTTGAT GAGTTATTTT TCTACAATGT CCACCTGACT 
CCTTCTGAAC TGATGGATGA GATCATCAGC ATTCGGGTTT ACAATTCCCA TTCTCTGCGG 
GCAGATTCTC TGATGGGGGA GTTTAAGATC GATGTTGGAT TTGTTTATGA TGAACCTGGT 
CATGCTGTCA TGAGGAAATG GCTTCTCCTC AATGACCCTG AAGATACCAG CTCGGGTGCT 
AAAGGCTACC TGAAAGTCAG CATGTTCGTC CTGGGAACTG GAGACGAGCC TCCTCCTGAG 
AAACAAGATC GTGATAATGA CAGTGATGAC GTGGAAAGTA ACTTGTTACT GCCTGCTGGC 
ATTGCCCTGC GGTGGGTGAC CTTCCTGCTG AAGATCTACA GAGCGGAGGA CATCCCCCAG 
ATGGATGATG CCTTCTCACA GACAGTGAAG GAAATATTTG GAGGCGTCGC TGATAAGAAG 
AACCTAGTGG ACCCTTTTGT AGAAGTTTCC TTTGCCGGAA AAAAGGTTTG CACGAACATA 
ATTGAGAAAA ATGCAAACCC TGAGTGGAAT CAAGTCGTTA ATCTTCAGAT CAAGTTTCCT 
TCAGTGTGTG AAAAAATAAA ACTGACAATA TATGACTGGG ACCGTCTTAC CAAAAATGAT 
GTTGTTGGAA CAACGTATCT GCACCTCTCT AAAATTGCTG CCTCTGGTGG GGAAGTGGAA 
GATTTCTCAT CCTCGGGATC TGGGGCTGCA TCGTATACAG CGAATACAGG AGAAACAGAG 
GTGGGCTTTG TTCCAACTTT TGGACCTTGT TATCTGAACC TTTATGGAAG CCCCCGAGAG 
TACACGGGAT TCCCAGATCC CTATGATGAG CTAAATTCTG GAAAGGGGGA AGGAGTTGCC 
TACAGAGGCA GAATCTTGGT TGAATTAAGC ACTTTACTTG AAAAGACACC ACCAGATAAA 
AAGCTTGAGT CCATTTCGCA TGATGACCTG CTGGTTGTTG AGAAATATCA GCGAAGGCGG 
AAGTACAGCC TGTCTGCTGT GTTCCATTCA GCCACCATGT TGCAAGACAT TGGCGAGGCC 
ATTCAGTTTG AAGTCAGCAT CGGGAACTAC GGCAACAAGT TTGACACGAC TTGTAAGCCT 
CTGGCATCAA CCACTCAGTA TAGCCGTGCT GTATTTGATG GAAACTACTA TTACTACTTG 
CCTTGGGCCC ACACGAAGCC AGTTGTTACA CTGACTTCCT ACTGGGAGGA TATTAGTCAT 
CGCCTGGATG CTGTGAACAC GCTCCTAGTC ATGGCAGAAA GGCTGCAATC AAACATAGAG 
GCTCTAAAAG CAGGGATACA AGGTAAAATT CCTTCAAACG AACTGGCAAA AATGTGGCAT 
AAGCTCATGG ATGAAGTTAT CGAAGACACA AGATACACGT TACCTCTCAC AGAAGGAAAA 
GCTAATGTCA CGGTTCTCGA TACTCAGATC CGAAAGTTGC GATCCAGAGC TCTCTCTCAG 
ATACATGAGG CAGCTGTGAG GATGCGGTCT GAGGTCCCAG ACGTCAAGTC CACATTGGTG 
GAAATTGAGG ACTGGCTCGA TAAATTAATG CAGCTGACTG AGGAGCCTCA GAACAGCATG 
CCTGACATCA TCATCTGGAT GATCCGGGGA GAGAAGAGAT TGGCCTACGC ACGCATTCCC 
GCGCATCAGG TTTTATACTC CACCAGCGGC GGGGATGCGT CTGGGAAGTA CTGTGGGAAA 
ACCCAGACCA TCTTTCTGAA GTATCCACAA GAAAAAAACA ACGGACCAAA GGTGCCCGTG 
GAGTTGCGTG TGAACATCTG GCTGGGCTTA AGTGCTGTTG AGAAGAAGTT CAATAGCTTC 
GCAGAAGGAA CATTCACCGT CTTTGCTGAA ATGTATGAAA ATCAAGCTCT CATGTTTGGA 
AAATGGGGCA CTTCCGGATT AGTGGGTCGT CATAAATTTT CCGATGTCAC AGGAAAAATA 
AAACTCAAGA GGGAATTCTT TTTGCCTCCA AAAGGCTGGG AATGGGAAGG AGACTGGATA 
GTTGATCCTG AAAGAAGCTT GCTGACCGAG GCGGATGCGG GTCACACAGA GTTCACAGAT 
GAAGTGTACC AGAACGAGAG TCGCTACCCC GGGGGCGAGT GGAAGCCGGC GGAGGACACC 
TACACCGATG CGAACGGCGA TAAAGCAGCA TCACCAAGCG AGCTCACTTG CCCTCCAGGC 
TGGGAATGGG AGGATGATGC ATGGGTCTAT GACATTAATC GTGCAGTGGA TGAGAGAGGC 
TGGGAATATG GAATTACCAT TCCTCCTGAT AATAAGCCCA AATCCTGGGT TGCAGCAGAA 
AAAATGTATC ATACTCATAG ACGCCGAAGG CTGGTCCGAA AACGCAAGAA AGATTTAACA 
CAATCCACTT CGAGTACCGC AAGGGCCATG GCAGAATTTG AAGACCGGGA GGGCTGGGAA 
TACGCTTCTC TAATTGGCTG GAAATTTCAC TGGAAACAGC GTAGTTCAGA TACGTTTCGC 
CGCAGACGCT GGAGGAGAAA AATGGCTCCT TCAGAAACAC ATGGTGCAGC TGCCATCTTT 
AAACTTGAAG GGGCCCTTGG CGCAGACACT ACGGACGATG GAGAGGAAAA GAGCGCAGAA 
AAGCAGAAGC ACAGCGCCAC CACTGTATTT GGAGCAAACA CTCCCATTGT TTCCTGTAAT 
TTTGACAGAG TCTACATTTA CCACCTGCGC TGCTACATCT ATCAAGCCAG GAACCTCATG 
GCTCTAGACA AGGATAGCTT TTCAGATCCA TATGCTCATG TCTCCTTCCT TCATCGAAGT 
AAAACCACCG AGATCATCCA CTCAACTCTG AATCCGACAT GGGACCAAAC GATTATATTC 
GATGAAATTG AAATTTATGG GGAACCCCAA ACCATCCTAC AGAACCCACC CAAGGTTATC 
ATCGAACTTT TTGACAATGA CCAAGTGGGC AAAGATGAAT TTTTAGGACG AAGCATGTGC 
TCTCCTCTGG TGAAACTAGA CTCAGAAATG GACATCACTC CCAAACTTCT CTGGTACCCA 
GTGATGAATG GAGACAAAAC CTGTGGGGAT GTCCTTGTAA CTGCAGAGTT GATTCTGAGG 
CACAAGGATG GCTCCAACCT TCCCATTCTT CCCTCGCAAA GGGCACCAAA TCTGTACATG 
GTCCCCCAGG GCATCAGGCC TGTGGTTCAG CTCACTGCTA TTGAGATTCT AGCTTGGGGC 
TTAAGAAATA TGAAGAATTA CCAGATGGCT TCTGTCACCT CCCCCAGTCT CATTGTGGAG 
TGTGGAGGGG AAAGGGTCGA GTCAGTGGTG ATCAAAAACC TGAAGAAGAC ACCCAACTTC 
CCAAGTTCTG TTCTCTTCAT GAGAGTGTTC TTGCCCAAGG AGGAACTGTA CATGCCTCCA 
CTGGTGATCA AGGTCATCGA CCACCGGCAG TTCGGGCGCA AGCCCGTTGT GGGCCAGTGC 
ACCATTGATC GCCTGGACCG CTTTCGCTGT GACCCTTATG CAGGAAAGGA GGACATCGTG 
CCACAGCTAA AAGCCTCCTT TTTGTCTACC CCACCCCGCC GGGACATTGT TATTGAAATA 
GAGGACACCA AGCCATTACT AGCTTCAAAG TGCTTAAGCA GTATGTCAAC AGCACTCAGC 
AAAATGGCTT CTCCAAGGAC AGTGCATCTA ACAGAAAAGG AGGAAGAAAT TGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CACAGGGGAA CATGAAAAAT GTGGACAATA TATTCAGAAA 
GGTTATTCCA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAGAGGA ATTCGAGGGC 
CTGACGGACT TCTCAGATAC TTTCAAGCTG TTTCGAGGCA AATCAGATGA GAATGAAGAC 
CCTTCTGTGG TAGGGGAGTT TAAGGGCTCC TTCCGAATCT ACCCACTGCC GGATGACCCC 
AGCGTGCCGG CCCCTCCCCG GCAGTTTCGG GAGTTACCTG ACAGCGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTAA TTGAAGACCG AGACAACTAC 
ATCCCCAACA CTCTCAACCC GGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAAAAGG ACCTGAAAAT TTCTGTCTAC GATTATGACA CCTTTACCCG TGATGAAAAA 
GTAGGAGAAA CGATCATTGA CCTAGAAAAC CGATTCCTTT CCCGGTTTGG GTCCCACTGT 
GGCATTCCTG AGCAGTACTG CGTTTCTGGA GTAAACACCT GGCGAGATCA ATTGAAACCA 
ACACAGTTGC TTCAAAATGT GGCCAGATTC AAAGGCTTCC CACCGCCCGT TCTTTCTGAA 
GATGGAAGTA GCATCAGATA TGGAGGAAAA GACTACGGCC TAGATGAATT TGAAGCAAAC 
AAAATCCTGC ACCAGCACCT TGGGCCCCCT GAGGAGCGCC TTGCCCTCTA CATCCTCAGG 
ACTCAGGGGC TGGTTCCTGA ACACGTGGAA ACGAGGACTT TGCACAGCAG CTTCCAGCCT 
AACATCTCCC AGGGAAGACT TCAGATGTGG GTGGATGTTT TCCCCAAGAA TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATT 
ATCTGGAACA CCAAGGATGT TGTCCTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGC 
GACATCTATG TGAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGGT CCTTGGATGG CGTAGGGAAC TTTAACTGGA GATTCGTCTT CCCTTTTGAC 
TACCTCCCAG CTGAACAGCT CTGTCTCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACTGAAT TTCGGGTCCC ACCCAGACTG GTCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTGGAA CTTGATTTGC ATCATACCAT CATTCCAGCA 
AAGTCACCAG AGAAATGCAG TTTGAACATG ATTCCAGACC TCAAAGCCAT GAACCCCCTG 
AAAGTGAAAA CTGCCTCTCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCTTGCTAC 
ACAGAGAAAG ATGGCTCCCG TGTGATGGCT GGGAAAGTGG AGATGACGCT GGAAGTCCTC 
AATGAGAAGG AGGCCGACGA GAGGCCAGCT GGGAAGGGAC GGGATGAACC CAACATGAAC 
CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCGTGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGA TTTAAGTGGG TCATCATTGC CCTGCTGATC 
CTGCTGATCC TGCTGTTCTT TGTGGCTGTG TTTCTGTATT CCTTACCGAA CTATTTGTCA 
ATGAAGATTG TGAAACCAAA TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_001193212.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180141.1 Bos taurus myoferlin (MYOF), mRNA.

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CloneID	OBa216187
Clone ID Related Accession (Same CDS sequence)	NM_001193212.2
Accession Version	NM_001193212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	myoferlin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02058830.1, DAAA02058829.1 and DAAA02058828.1. On Jun 30, 2017 this sequence version replaced NM_001193212.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180141.1
CDS	90..6275
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGGDKEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	NM_001193212.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193212.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_001193212.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180141.2 Bos taurus myoferlin (MYOF), mRNA.

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CloneID	OBa239152
Clone ID Related Accession (Same CDS sequence)	NM_001193212.3
Accession Version	NM_001193212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529164800000
Organism	Bos taurus(cattle)
Product	myoferlin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000026.1, EV684499.1 and CB419566.1. On Jun 18, 2018 this sequence version replaced NM_001193212.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGACCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGTGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTCT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180141.2
CDS	90..6275
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGGDKEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	NM_001193212.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193212.3

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGACCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGTGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTCT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_001193212.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180141.1 Bos taurus myoferlin (MYOF), mRNA.

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CloneID	OBa40769
Clone ID Related Accession (Same CDS sequence)	NM_001193212.1
Accession Version	NM_001193212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1420214400000
Organism	Bos taurus(Cattle)
Product	myoferlin
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03042346.1, AAFC03125080.1, AAFC03073515.1, AAFC02005930.1, AAFC02115478.1, AAFC03073516.1 and AAFC03117136.1. On Jul 17, 2010 this sequence version replaced XM_873525.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAGGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCCG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACTTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180141.1
CDS	90..6275
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGGDKEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	NM_001193212.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus myoferlin (MYOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193212.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG 
GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG 
AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG 
ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC 
AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC 
ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT 
AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT 
CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG 
TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT 
GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC 
CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA 
GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG 
TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG 
ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG 
GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA 
CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT 
GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA 
TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT 
GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC 
CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG 
AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC 
CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT 
GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC 
CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT 
AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA 
GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT 
CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG 
CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA 
TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC 
AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT 
CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG 
GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG 
GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC 
TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA 
GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG 
GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG 
GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA 
GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT 
GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC 
CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA 
GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA 
ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA 
CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA 
GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT 
GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG 
GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG 
ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA 
GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA 
GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC 
CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG 
TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT 
TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC 
ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA 
TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT 
TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT 
AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG 
CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT 
TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC 
CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC 
GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG 
GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT 
GGAGGAAAAG ACTACGGCCT AGATGAATTT GAGGCAAACA AAATCCTGCA CCAGCACCTT 
GGGCCCCCCG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA 
CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACTTG CGTGTGATTA TCTGGAACAC CAAGGATGTT 
GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC 
GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC 
TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA 
CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT 
TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT 
GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG 
AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT 
GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_024985587.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841355.1 Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

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CloneID	OBa230831
Clone ID Related Accession (Same CDS sequence)	XM_024985587.1
Accession Version	XM_024985587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGAGGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC 
CCCGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG 
GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC 
CGGAAAGCCA AGAAATACTA CTTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG 
GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT 
GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG 
AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC 
GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA 
CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG 
GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC 
ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG 
CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG 
GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA 
GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA 
GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC 
CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT 
GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841355.1
CDS	248..6487
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_024985587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985587.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGAGGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC 
CCCGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG 
GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC 
CGGAAAGCCA AGAAATACTA CTTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG 
GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT 
GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG 
AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC 
GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA 
CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG 
GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC 
ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG 
CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG 
GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA 
GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA 
GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC 
CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT 
GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_024985588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841356.1 Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

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CloneID	OBa230832
Clone ID Related Accession (Same CDS sequence)	XM_024985588.1
Accession Version	XM_024985588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG 
GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT 
AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT 
TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG 
GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT 
GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG 
CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT 
AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC 
CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC 
AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA 
AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA 
GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT 
CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG 
TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA 
AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAGGC AAACAAAATC 
CTGCACCAGC ACCTTGGGCC CCCCGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG 
GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC 
TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC 
CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACTTGCGTGT GATTATCTGG 
AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC 
TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC 
AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC 
CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT 
GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG 
GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA 
CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG 
AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG 
AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG 
AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG 
CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC 
ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG 
ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG 
ATTGTGAAAC CAAATGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841356.1
CDS	249..6449
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_024985588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985588.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG 
GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT 
AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT 
TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG 
GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT 
GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG 
CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT 
AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC 
CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC 
AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA 
AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA 
GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT 
CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG 
TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA 
AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAGGC AAACAAAATC 
CTGCACCAGC ACCTTGGGCC CCCCGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG 
GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC 
TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC 
CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACTTGCGTGT GATTATCTGG 
AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC 
TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC 
AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC 
CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT 
GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG 
GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA 
CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG 
AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG 
AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG 
AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG 
CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC 
ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG 
ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG 
ATTGTGAAAC CAAATGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_024985589.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841357.1 Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

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CloneID	OBa230833
Clone ID Related Accession (Same CDS sequence)	XM_024985589.1
Accession Version	XM_024985589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA 
GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT 
GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC 
AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG 
GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG 
TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG 
TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT 
GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA 
GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT 
TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC 
AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA 
GGAAAAGACT ACGGCCTAGA TGAATTTGAG GCAAACAAAA TCCTGCACCA GCACCTTGGG 
CCCCCCGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC 
GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACTTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC 
CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA 
GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT 
CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC 
AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG 
AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG 
ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG 
CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG 
GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841357.1
CDS	248..6430
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_024985589.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985589.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA 
GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT 
GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC 
AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG 
GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG 
TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG 
TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT 
GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA 
GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT 
TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC 
AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA 
GGAAAAGACT ACGGCCTAGA TGAATTTGAG GCAAACAAAA TCCTGCACCA GCACCTTGGG 
CCCCCCGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC 
GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACTTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC 
CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA 
GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT 
CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC 
AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG 
AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG 
ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG 
CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG 
GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_024985591.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841359.1 Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

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CloneID	OBa230834
Clone ID Related Accession (Same CDS sequence)	XM_024985591.1
Accession Version	XM_024985591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT 
TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG 
ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT 
TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC 
GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC 
CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA 
GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC 
ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA 
CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT 
GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA GGCAAACAAA 
ATCCTGCACC AGCACCTTGG GCCCCCCGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT 
CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC 
ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACTTGCG TGTGATTATC 
TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC 
CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG 
TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA 
GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA 
GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT 
GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC 
AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG 
CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG 
AAGATTGTGA AACCAAATGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841359.1
CDS	248..6391
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKVKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_024985591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985591.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT 
TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG 
ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT 
TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC 
GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC 
CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA 
GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC 
ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA 
CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT 
GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA GGCAAACAAA 
ATCCTGCACC AGCACCTTGG GCCCCCCGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT 
CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC 
ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACTTGCG TGTGATTATC 
TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC 
CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG 
TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA 
GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA 
GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT 
GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC 
AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG 
CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG 
AAGATTGTGA AACCAAATGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_005225521.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225578.1 Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

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CloneID	OBa33555
Clone ID Related Accession (Same CDS sequence)	XM_005225521.3
Accession Version	XM_005225521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGAAGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC 
CCTGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG 
GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC 
CGGAAAGCCA AGAAATACTA CCTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG 
GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT 
GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG 
AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC 
GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA 
CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG 
GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC 
ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG 
CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG 
GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA 
GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA 
GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC 
CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT 
GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225578.1
CDS	240..6479
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_005225521.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225521.3

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT 
CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG 
GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT 
TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG 
CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC 
TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT 
CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC 
TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG 
GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC 
GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC 
TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA 
AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT 
GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA 
TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA 
AAAGACTACG GCCTAGATGA ATTTGAAGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC 
CCTGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG 
GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC 
CGGAAAGCCA AGAAATACTA CCTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG 
GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT 
GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG 
AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC 
GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA 
CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG 
GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC 
ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG 
CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG 
GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA 
GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA 
GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC 
CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT 
GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_010819883.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818185.1 Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

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CloneID	OBa48674
Clone ID Related Accession (Same CDS sequence)	XM_010819883.2
Accession Version	XM_010819883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG 
GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT 
AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT 
TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG 
GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT 
GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG 
CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT 
AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC 
CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC 
AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA 
AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA 
GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT 
CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG 
TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA 
AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAAGC AAACAAAATC 
CTGCACCAGC ACCTTGGGCC CCCTGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG 
GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC 
TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC 
CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACCTGCGTGT GATTATCTGG 
AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC 
TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC 
AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC 
CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT 
GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG 
GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA 
CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG 
AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG 
AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG 
AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG 
CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC 
ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG 
ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG 
ATTGTGAAAC CAAATGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818185.1
CDS	240..6440
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_010819883.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819883.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG 
GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT 
AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT 
TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG 
GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT 
GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG 
CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT 
AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC 
CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC 
AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA 
AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA 
GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT 
CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG 
TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA 
AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAAGC AAACAAAATC 
CTGCACCAGC ACCTTGGGCC CCCTGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG 
GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC 
TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC 
CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACCTGCGTGT GATTATCTGG 
AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC 
TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC 
AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC 
CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT 
GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG 
GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA 
CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG 
AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG 
AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG 
AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG 
CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC 
ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG 
ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG 
ATTGTGAAAC CAAATGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_010819884.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818186.1 Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

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CloneID	OBa48675
Clone ID Related Accession (Same CDS sequence)	XM_010819884.2
Accession Version	XM_010819884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA 
GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT 
GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC 
AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG 
GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG 
TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG 
TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT 
GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA 
GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT 
TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC 
AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA 
GGAAAAGACT ACGGCCTAGA TGAATTTGAA GCAAACAAAA TCCTGCACCA GCACCTTGGG 
CCCCCTGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC 
GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC 
CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA 
GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT 
CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC 
AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG 
AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG 
ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG 
CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG 
GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818186.1
CDS	240..6422
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGSGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_010819884.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819884.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT 
GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT 
TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT 
GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA 
AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC 
CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT 
TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC 
TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT 
GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT 
ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA 
GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA 
ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC 
ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG 
ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG 
TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA 
ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG 
GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC 
GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA 
CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC 
TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT 
GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT 
CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT 
CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC 
GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC 
CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA 
GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC 
TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT 
GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA 
AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC 
ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT 
CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC 
ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA 
TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG 
CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT 
CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT 
CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC 
CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG 
GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA 
ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG 
GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT 
CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT 
CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG 
GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG 
GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG 
TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG 
CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC 
TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT 
ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA 
AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA 
GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT 
GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC 
AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG 
GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG 
TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG 
TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT 
GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA 
GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT 
TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC 
AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA 
GGAAAAGACT ACGGCCTAGA TGAATTTGAA GCAAACAAAA TCCTGCACCA GCACCTTGGG 
CCCCCTGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC 
GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC 
CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA 
GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT 
CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC 
AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG 
AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG 
ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG 
CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG 
GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_010819885.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818187.1 Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

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CloneID	OBa48676
Clone ID Related Accession (Same CDS sequence)	XM_010819885.2
Accession Version	XM_010819885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT 
TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG 
ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT 
TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC 
GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC 
CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA 
GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC 
ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA 
CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT 
GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA AGCAAACAAA 
ATCCTGCACC AGCACCTTGG GCCCCCTGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT 
CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC 
ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATTATC 
TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC 
CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG 
TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA 
GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA 
GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT 
GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC 
AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG 
CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG 
AAGATTGTGA AACCAAATGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818187.1
CDS	240..6383
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLEIVVKDFETIGQNKLIGTATIALKDLMGDQSRSLPYKLVSLLNERGQETGATIDLVIGYHPPSAPHPNDPGGTSVPGTGEEEEDGGDEDMLDSTVRGPGPRGPGGTVPEAHLARRLTKAKSSRRMLSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHLTPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYLKVSMFVLGTGDEPPPEKQDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGVADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELSTLLEKTPPDKKLESISHDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEALKAGIQGKIPSNELAKMWHKLMDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRALSQIHEAAVRMRSEVPDVKSTLVEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGDASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGEWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWVYDINRAVDERGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQSTSSTARAMAEFEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTDDGEEKSAEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGEPQTILQNPPKVIIELFDNDQVGKDEFLGRSMCSPLVKLDSEMDITPKLLWYPVMNGDKTCGDVLVTAELILRHKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIVECGGERVESVVIKNLKKTPNFPSSVLFMRVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDRLDRFRCDPYAGKEDIVPQLKASFLSTPPRRDIVIEIEDTKPLLASKLTEKEEEIVDWWSKFYASTGEHEKCGQYIQKGYSKLKIYDCELEDVEEFEGLTDFSDTFKLFRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDNYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLKPTQLLQNVARFKGFPPPVLSEDGSSIRYGGKDYGLDEFEANKILHQHLGPPEERLALYILRTQGLVPEHVETRTLHSSFQPNISQGRLQMWVDVFPKNLGPPGPPFNITPRKAKKYYLRVIIWNTKDVVLDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGVGNFNWRFVFPFDYLPAEQLCLVAKKEHFWSIDQTEFRVPPRLVIQIWDNDKFSLDDYLGFLELDLHHTIIPAKSPEKCSLNMIPDLKAMNPLKVKTASLFEQKSMKGWWPCYTEKDGSRVMAGKVEMTLEVLNEKEADERPAGKGRDEPNMNPKLDPPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIALLILLILLFFVAVFLYSLPNYLSMKIVKPNA

Target ORF information:

RefSeq Version	XM_010819885.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819885.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT 
TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA 
ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC 
TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG 
GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG 
CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC 
AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG 
CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC 
CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA 
GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT 
GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC 
AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG 
ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC 
CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC 
GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT 
GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG 
CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG 
AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT 
TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG 
AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA 
ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC 
TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC 
TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG 
GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA 
GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT 
GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC 
AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG 
TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA 
TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG 
GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG 
GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA 
AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC 
ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT 
GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT 
GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT 
GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC 
ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT 
CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG 
ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG 
CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA 
GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG 
GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC 
AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT 
CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG 
TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC 
GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA 
TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA 
TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG 
TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC 
ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT 
TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA 
CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT 
GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG 
AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC 
AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA 
GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC 
ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA 
ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA 
CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT 
CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG 
AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG 
GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC 
CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA 
AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA 
GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT 
TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG 
ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT 
GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG 
CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC 
ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT 
TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG 
ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT 
TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC 
GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC 
CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA 
GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC 
ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA 
CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT 
GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA AGCAAACAAA 
ATCCTGCACC AGCACCTTGG GCCCCCTGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT 
CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC 
ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATTATC 
TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC 
CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG 
TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA 
GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA 
GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT 
GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC 
AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG 
CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG 
AAGATTGTGA AACCAAATGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYOF
Entrez Gene ID	513597
Full Name	myoferlin
Synonyms	FER1L3
General protein information	Preferred Names myoferlin Names myoferlin fer-1-like 3, myoferlin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Danio rerio (zebrafish)	myof	XP_687465.5
Macaca mulatta (Rhesus monkey)	MYOF	XP_001089235.2
Gallus gallus (chicken)	MYOF	XP_001231546.2
Homo sapiens (human)	MYOF	NP_038479.1
Pan troglodytes (chimpanzee)	MYOF	XP_003312803.1
Mus musculus (house mouse)	Myof	NP_001093104.1
Xenopus tropicalis (tropical clawed frog)	myof	NP_001122123.1
Bos taurus (cattle)	MYOF	NP_001180141.1
Rattus norvegicus (Norway rat)	Myof	NP_001178565.1
Canis lupus familiaris (dog)	MYOF	XP_858858.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYOF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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