Apaf-1 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apaf-1
Entrez Gene ID	100529085
Full Name	Apaf-1 protein
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012697713.2	XP_012553167.2	apaf-1 protein isoform X1
XM_021353580.1	XP_021209255.1	apaf-1 protein isoform X1
XM_012697713.1	XP_012553167.1	apaf-1 protein isoform X1
NM_001200008.1	NP_001186937.1	Apaf-1 protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.
Zhang JY, Pan MH, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C
BMC genomics11611(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Apaf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apaf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg00410	NM_001200008.1 Latest version!	Bombyx mori Apaf-1 protein (Apaf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBg14515	XM_012697713.1 Latest version!	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg22410	XM_012697713.2 Latest version!	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg22410	XM_021353580.1 Latest version!	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apaf-1 ( NM_001200008.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001186937.1 Bombyx mori Apaf-1 protein (Apaf-1), mRNA.

Price & Availability↑

CloneID	OBg00410
Clone ID Related Accession (Same CDS sequence)	NM_001200008.1
Accession Version	NM_001200008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498233600000
Organism	Bombyx mori(domestic silkworm)
Product	Apaf-1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HQ179968.1. ##Evidence-Data-START## Transcript exon combination :: HQ179968.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCCGGCT CCAAAAAATC CATGGCTGTA AAGTCGGCTG GAAAAGTGAT GGCTTCCGTC 
TTTTGGGATG CAGATGGGAT CTTGATGATA GATTACCTTC CTAAAGATGA GCGAGCCGCG 
ATCGAAATGG ATCTCAAGAA CAGGAAGCTG CTGCAGCACA ATCAGCAGGC TATCATCAAA 
GATCTAGATG TTCAGTACAT TCTAGACGAA CTTTACACGA AACAAGCTAT ATCTTCAGAG 
GTGTACGAAC ATATCGCTAA TCTGGTCATA GTTAAAAATC GAGTCGAAGG CGTCAGGTAT 
TTGATAGATT GCCTCCTAAG GTATGGCACG AATCAGGCCT ACGAAGCATT CGTTGATAGC 
CTTGCCAAAG ATTACAACTG GCTCTGGGAG AAGTTTGCTA CACAATCCAA CGGGCCAATG 
TTTAACGACA GCTTCGAGGA CAGCCTTAGC AAAGGCGACG TGCCAAGGCT TCCCGAACAC 
CATGTCAGGA GAAAGGCTGT GGAAACTGAA GTCCTATCCA AGATTAAAGA GTTGACCCGT 
CACAAGATAC TGGTGCTTCA CGGTATGCTT GGCAGCGGAA AGACCTGCGT CGCCATCAAC 
GTACTCCGGG ACAACCCCGA ACTCATAACG AACAATTTCA ATGATATTGT CTTTTGGATG 
AACTTTGCGA ATTGCAAGAC CGATGACGAC ATCATCGCTC AACAGAATAC GTTACTACGT 
AAAATATCAT CGATGTACAT TCAAAATTCT CACATGAACT CTTCGATATC AATGTCGAGT 
ATGGGCTCGA ATATCGACAG CCATTCGCTC TCCTACTACG ATCGCACGTG GAACGACTTC 
AGAGACAGAC TGAAGAACCT ATTCTCGGAG CCCCCGCTGA AGGAAGGTTT GCTCGTAATG 
GACGAAGTCA ACGATCGGAA ATGCGTCGAA GCGTTCGATA TCGGTTGCAA GATACTGATC 
ACGACCAGAG ATACGGACGT TGTGGCTAAT TATCACCCGC AAATCGTTAC GGTGGAGAAC 
AGTTTCACAG AGAGGGAGTC CCTAGAACTG TTTGCTTCTT GCTTAGACGA ACCTGTATCG 
AAACTTCCAT CGTATGCGAA AGAGCTCAAC GAATTATGCA AAGGAAGCCC CTTCCACGTG 
GCCCTGATCG GTGCCCAGCT CGCAGAAGAC AGGGAGAGAC TGAAACACGA TCGAAGTCAT 
TGGAAATATT ACTTGAAGAA ATTAAAAAAG AATTTCTTTT CTGTGTGGAA AACGAACAAC 
GATAACCCGA TGAAAACTAT ACAAGTGTGT ATAAACTCGT TGAACCCCAA CACGCTTGCG 
ATGTTCATGA AACTGGCAGT GCTTCCGGAC AGCGTCAAAG TCACCGCTCA AGTCTACAGC 
AAACTGTGGA ACGTAGACGT CAACGAAGTC GAAAACATAA CGAAGAAACT CAAGAGCAAG 
TCTCTGATCA TAAAGAACTA CGATAGAGAT CAAAAAAAGT ATTTATACGA AGTGCACGAT 
CTCATAATGA ACTATCTAAG GTCTTGTACG CACGAAGAGG AAATGAAGAA GCTACACGAC 
GAACTGTTGA ACAGTTACAG ATATGATAGC GAAACTGCAC CAACGGAGAT AGTCGATGAC 
GGATACATAG CCTTCTACAT AGGATTTCAC ATTTTGAAAA CGAAAAATTT GCACCAGAAA 
TGGAGTTTGT TCAGTAAGCT GTATTTAGAT TTGAGGTTTC TAGGGAATAA AGTCAGACTG 
ACCGGACCGG ACGATGTCAT GCTGGATCTG CAGAAATACG AAAATTATAT TGTGAGAGAT 
GCTTCTTGTG ACTTTGGTGT GACGAGGAAG TGGAAGTTGC TTGTTTTCAA AGATGGGATC 
TGTCGCTCGT TCTTCAACAT AGAAACTAAT AAGCAGAAGA AGGAACTAAA GGGCACCGGT 
GCCGCCATTA AATGGATAGG CGTCGCGTGC ACTAAAGACA CGACTATTGT AGCCGCTCTG 
TCGACGGACG GATATCTCAA ACTTTGGTTC GTCGATGACT TCGATGATGA CGACAACGCT 
AATAATCTCG TCGAAGAAGA ACCGAACGAT GCCTACAACA ACTATCCCAG TCCGTTAACA 
TTTGAACCCA AACTGGGACC GATACTAAAC TGTCGTTGGA CGACCAGCGA AGACTTGTTA 
ATAACTCACA CGTCACAAAT GATTATACTG TACCGTCCCA AAGGCGCCGA GTATGACGTC 
ATAAGTGAGT TCGACAGGGA ACAGAAGCTG CTATGCTGCG TCCCCTGTAA CAACGACAAA 
TACATTGCTG TAGCTAGTTT CAACGCCGTA TACAGAGTGG ACTTGATCGA CATTAGAACT 
AAGCAGAAAG TTGTTTATTT CGAAGAAACA GGTGCCATAT TGGATATGCT TGTTGTTCCT 
GGTACCAATA AAATAATAAC GCTGAAACAA AACGAGATAA TGGCACACGA GTTCCGGGTA 
AAGCCTGCTT CGATAAACAG AGGCGGGACG GTTATCGATA GCGGTGCGGT CAAAGCGGAT 
GTCCGGTTCG CGGCGATGGC CGTCAACAAC TCGGGCTCGC TGCTGTTCGC GGCCACGGAC 
GATAGCCGGG TCGTTTGTGT CGACTTGAAA ACTAACTCAA GAGTTTTCGA TATCGAGAAC 
AGAAGGGGCA ATGTGGTGTC AATGGCGGTG AGCGAAGTGC TGATGTGGGA TGAATTCGAA 
CCGAGCTCCG ACGTACTTCT AACCGGTACT GATAGTACCG AAAATTCTGC CAAAGTTTGG 
TATTTAGACG CTTCATACGT GTCGCAGACT GCACAAAGAA AAGGAAAAGT TCGTCTAACA 
AGTAAATTCG ACGTGAGTTT CCTAAACGCG ACAACGCCAA CATCTCCGAG CGCCTCGCCT 
CTGAACAGCG GCGTGGAAAC AGTTCACAAC CCTCTAAAAT CACACCAGTC GTTCACGAAC 
AAGGACCCAG TAAAGAAACC GATTAAAACT ACCATGAGCT TGGACAGGAA TACATTGAAA 
CCGCTCAATC TCAAAGGAAT ATGCAACAAC AATGAAGGGC TCATACACCC ATTACTGGCT 
GTCGTCGATG ATATGAACAA TATACAGGTG ATGCGTGGTA AGAAAGTCCT AACCGAGATC 
TTAGAGAAAT CTGATGAGAA GATAACAGCC GTCAAGGTAT CGCCGTGCAA TCACTACATC 
ATGTACGGGC TCAAGTCGGG AAAAGTCAAG AGATACACAT TGAGAACCAA GGAGATGACA 
ACTATAATGC ACGTTAACAG TCCGGTGCAA TACCTGAACT ACATCAACAA CAGGTTGATG 
CTGGTCGCGT GGAAGAACCG ATGTCTAATG TCGTATAAAC TGGGCCACGA GGAGACCTGG 
AAGACTGAAA TGCTACAGGC CGGTAACACG CACCTCGGCT CGCAAGAATC GCAGCTAGGA 
AGCGATTGGC AGAGTATGAA AATAAGGAAC GGCCACTCCG ACTTGTCGAC GTCGAGCAGC 
GAGGCCAGCG TGAGCTCCCG CGAGAACCGA CTGTTCCACG GCGGGGAGAT GAAGGAGCTG 
TACAAACAGA GCGACCTCGT CGACTGCTTC TGGGTCGCGG ACACCGGCCT CATCACGGTC 
GAGTCGAACG CGGCCATCAA ACTGTGGAAC AAAGACTGCA AAGTCTCAAC CATATTGAAC 
GCGAGGCAAA TGGATACTTA CATCACGTGC GCCGCTTTCC AGAACAACGT TCTCGTTATA 
TGCGATAGTG AAAACAAAGG ATTTCAGTCG TACGAACTAA AATTCGACAA CACAATAGAT 
TTGTGCCTCA TCCAAGAGTA CAAACTGAAC AATGTGGTGA CGTCATGCGA TATCACATCC 
GACGGCTACA TACTAGCGAT GGGACTAGAC TGTGGTAATG TTGTGCTTTG GAACGTAAAA 
GGGAAGCGTC AAATCAGTTT ACTCAAACAC CACAACTCGA GAGTGCAGGC GTGCTCGTTC 
TCGCCGGTAC CGGACCGACT GTACCGGAGC TCCGTGCACT CGCCCGGTGT GACGTCACCG 
GCGGACGCCG TCGCCGACGA CCAGCCTCCA CTGGTCCTGG TCACGATGGC TTCAGAGATA 
GTCTGGTGGA ACATCACGTA CGTCATACGG ATACGAGCGA GTAATAAGTC ATTGTGGAGA 
TGTAAAGAAG GGTCGAAACG CAAAGAAATA TTGGCGTGTA TAAAACTATC GGGTATAAAC 
GCGACGAGGT TGTGCCACGA CGAGAATTTC TCGTGCTTCG TGACCGTGGA CAATCCGGGC 
CACGTCCACA TCATGAACGT TATGAGGGAT CTTTGTACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186937.1
CDS	1..4302
Translation	MPGSKKSMAVKSAGKVMASVFWDADGILMIDYLPKDERAAIEMDLKNRKLLQHNQQAIIKDLDVQYILDELYTKQAISSEVYEHIANLVIVKNRVEGVRYLIDCLLRYGTNQAYEAFVDSLAKDYNWLWEKFATQSNGPMFNDSFEDSLSKGDVPRLPEHHVRRKAVETEVLSKIKELTRHKILVLHGMLGSGKTCVAINVLRDNPELITNNFNDIVFWMNFANCKTDDDIIAQQNTLLRKISSMYIQNSHMNSSISMSSMGSNIDSHSLSYYDRTWNDFRDRLKNLFSEPPLKEGLLVMDEVNDRKCVEAFDIGCKILITTRDTDVVANYHPQIVTVENSFTERESLELFASCLDEPVSKLPSYAKELNELCKGSPFHVALIGAQLAEDRERLKHDRSHWKYYLKKLKKNFFSVWKTNNDNPMKTIQVCINSLNPNTLAMFMKLAVLPDSVKVTAQVYSKLWNVDVNEVENITKKLKSKSLIIKNYDRDQKKYLYEVHDLIMNYLRSCTHEEEMKKLHDELLNSYRYDSETAPTEIVDDGYIAFYIGFHILKTKNLHQKWSLFSKLYLDLRFLGNKVRLTGPDDVMLDLQKYENYIVRDASCDFGVTRKWKLLVFKDGICRSFFNIETNKQKKELKGTGAAIKWIGVACTKDTTIVAALSTDGYLKLWFVDDFDDDDNANNLVEEEPNDAYNNYPSPLTFEPKLGPILNCRWTTSEDLLITHTSQMIILYRPKGAEYDVISEFDREQKLLCCVPCNNDKYIAVASFNAVYRVDLIDIRTKQKVVYFEETGAILDMLVVPGTNKIITLKQNEIMAHEFRVKPASINRGGTVIDSGAVKADVRFAAMAVNNSGSLLFAATDDSRVVCVDLKTNSRVFDIENRRGNVVSMAVSEVLMWDEFEPSSDVLLTGTDSTENSAKVWYLDASYVSQTAQRKGKVRLTSKFDVSFLNATTPTSPSASPLNSGVETVHNPLKSHQSFTNKDPVKKPIKTTMSLDRNTLKPLNLKGICNNNEGLIHPLLAVVDDMNNIQVMRGKKVLTEILEKSDEKITAVKVSPCNHYIMYGLKSGKVKRYTLRTKEMTTIMHVNSPVQYLNYINNRLMLVAWKNRCLMSYKLGHEETWKTEMLQAGNTHLGSQESQLGSDWQSMKIRNGHSDLSTSSSEASVSSRENRLFHGGEMKELYKQSDLVDCFWVADTGLITVESNAAIKLWNKDCKVSTILNARQMDTYITCAAFQNNVLVICDSENKGFQSYELKFDNTIDLCLIQEYKLNNVVTSCDITSDGYILAMGLDCGNVVLWNVKGKRQISLLKHHNSRVQACSFSPVPDRLYRSSVHSPGVTSPADAVADDQPPLVLVTMASEIVWWNITYVIRIRASNKSLWRCKEGSKRKEILACIKLSGINATRLCHDENFSCFVTVDNPGHVHIMNVMRDLCT

Target ORF information:

RefSeq Version	NM_001200008.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori Apaf-1 protein (Apaf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200008.1

ORF Insert Sequence:

ATGCCCGGCT CCAAAAAATC CATGGCTGTA AAGTCGGCTG GAAAAGTGAT GGCTTCCGTC 
TTTTGGGATG CAGATGGGAT CTTGATGATA GATTACCTTC CTAAAGATGA GCGAGCCGCG 
ATCGAAATGG ATCTCAAGAA CAGGAAGCTG CTGCAGCACA ATCAGCAGGC TATCATCAAA 
GATCTAGATG TTCAGTACAT TCTAGACGAA CTTTACACGA AACAAGCTAT ATCTTCAGAG 
GTGTACGAAC ATATCGCTAA TCTGGTCATA GTTAAAAATC GAGTCGAAGG CGTCAGGTAT 
TTGATAGATT GCCTCCTAAG GTATGGCACG AATCAGGCCT ACGAAGCATT CGTTGATAGC 
CTTGCCAAAG ATTACAACTG GCTCTGGGAG AAGTTTGCTA CACAATCCAA CGGGCCAATG 
TTTAACGACA GCTTCGAGGA CAGCCTTAGC AAAGGCGACG TGCCAAGGCT TCCCGAACAC 
CATGTCAGGA GAAAGGCTGT GGAAACTGAA GTCCTATCCA AGATTAAAGA GTTGACCCGT 
CACAAGATAC TGGTGCTTCA CGGTATGCTT GGCAGCGGAA AGACCTGCGT CGCCATCAAC 
GTACTCCGGG ACAACCCCGA ACTCATAACG AACAATTTCA ATGATATTGT CTTTTGGATG 
AACTTTGCGA ATTGCAAGAC CGATGACGAC ATCATCGCTC AACAGAATAC GTTACTACGT 
AAAATATCAT CGATGTACAT TCAAAATTCT CACATGAACT CTTCGATATC AATGTCGAGT 
ATGGGCTCGA ATATCGACAG CCATTCGCTC TCCTACTACG ATCGCACGTG GAACGACTTC 
AGAGACAGAC TGAAGAACCT ATTCTCGGAG CCCCCGCTGA AGGAAGGTTT GCTCGTAATG 
GACGAAGTCA ACGATCGGAA ATGCGTCGAA GCGTTCGATA TCGGTTGCAA GATACTGATC 
ACGACCAGAG ATACGGACGT TGTGGCTAAT TATCACCCGC AAATCGTTAC GGTGGAGAAC 
AGTTTCACAG AGAGGGAGTC CCTAGAACTG TTTGCTTCTT GCTTAGACGA ACCTGTATCG 
AAACTTCCAT CGTATGCGAA AGAGCTCAAC GAATTATGCA AAGGAAGCCC CTTCCACGTG 
GCCCTGATCG GTGCCCAGCT CGCAGAAGAC AGGGAGAGAC TGAAACACGA TCGAAGTCAT 
TGGAAATATT ACTTGAAGAA ATTAAAAAAG AATTTCTTTT CTGTGTGGAA AACGAACAAC 
GATAACCCGA TGAAAACTAT ACAAGTGTGT ATAAACTCGT TGAACCCCAA CACGCTTGCG 
ATGTTCATGA AACTGGCAGT GCTTCCGGAC AGCGTCAAAG TCACCGCTCA AGTCTACAGC 
AAACTGTGGA ACGTAGACGT CAACGAAGTC GAAAACATAA CGAAGAAACT CAAGAGCAAG 
TCTCTGATCA TAAAGAACTA CGATAGAGAT CAAAAAAAGT ATTTATACGA AGTGCACGAT 
CTCATAATGA ACTATCTAAG GTCTTGTACG CACGAAGAGG AAATGAAGAA GCTACACGAC 
GAACTGTTGA ACAGTTACAG ATATGATAGC GAAACTGCAC CAACGGAGAT AGTCGATGAC 
GGATACATAG CCTTCTACAT AGGATTTCAC ATTTTGAAAA CGAAAAATTT GCACCAGAAA 
TGGAGTTTGT TCAGTAAGCT GTATTTAGAT TTGAGGTTTC TAGGGAATAA AGTCAGACTG 
ACCGGACCGG ACGATGTCAT GCTGGATCTG CAGAAATACG AAAATTATAT TGTGAGAGAT 
GCTTCTTGTG ACTTTGGTGT GACGAGGAAG TGGAAGTTGC TTGTTTTCAA AGATGGGATC 
TGTCGCTCGT TCTTCAACAT AGAAACTAAT AAGCAGAAGA AGGAACTAAA GGGCACCGGT 
GCCGCCATTA AATGGATAGG CGTCGCGTGC ACTAAAGACA CGACTATTGT AGCCGCTCTG 
TCGACGGACG GATATCTCAA ACTTTGGTTC GTCGATGACT TCGATGATGA CGACAACGCT 
AATAATCTCG TCGAAGAAGA ACCGAACGAT GCCTACAACA ACTATCCCAG TCCGTTAACA 
TTTGAACCCA AACTGGGACC GATACTAAAC TGTCGTTGGA CGACCAGCGA AGACTTGTTA 
ATAACTCACA CGTCACAAAT GATTATACTG TACCGTCCCA AAGGCGCCGA GTATGACGTC 
ATAAGTGAGT TCGACAGGGA ACAGAAGCTG CTATGCTGCG TCCCCTGTAA CAACGACAAA 
TACATTGCTG TAGCTAGTTT CAACGCCGTA TACAGAGTGG ACTTGATCGA CATTAGAACT 
AAGCAGAAAG TTGTTTATTT CGAAGAAACA GGTGCCATAT TGGATATGCT TGTTGTTCCT 
GGTACCAATA AAATAATAAC GCTGAAACAA AACGAGATAA TGGCACACGA GTTCCGGGTA 
AAGCCTGCTT CGATAAACAG AGGCGGGACG GTTATCGATA GCGGTGCGGT CAAAGCGGAT 
GTCCGGTTCG CGGCGATGGC CGTCAACAAC TCGGGCTCGC TGCTGTTCGC GGCCACGGAC 
GATAGCCGGG TCGTTTGTGT CGACTTGAAA ACTAACTCAA GAGTTTTCGA TATCGAGAAC 
AGAAGGGGCA ATGTGGTGTC AATGGCGGTG AGCGAAGTGC TGATGTGGGA TGAATTCGAA 
CCGAGCTCCG ACGTACTTCT AACCGGTACT GATAGTACCG AAAATTCTGC CAAAGTTTGG 
TATTTAGACG CTTCATACGT GTCGCAGACT GCACAAAGAA AAGGAAAAGT TCGTCTAACA 
AGTAAATTCG ACGTGAGTTT CCTAAACGCG ACAACGCCAA CATCTCCGAG CGCCTCGCCT 
CTGAACAGCG GCGTGGAAAC AGTTCACAAC CCTCTAAAAT CACACCAGTC GTTCACGAAC 
AAGGACCCAG TAAAGAAACC GATTAAAACT ACCATGAGCT TGGACAGGAA TACATTGAAA 
CCGCTCAATC TCAAAGGAAT ATGCAACAAC AATGAAGGGC TCATACACCC ATTACTGGCT 
GTCGTCGATG ATATGAACAA TATACAGGTG ATGCGTGGTA AGAAAGTCCT AACCGAGATC 
TTAGAGAAAT CTGATGAGAA GATAACAGCC GTCAAGGTAT CGCCGTGCAA TCACTACATC 
ATGTACGGGC TCAAGTCGGG AAAAGTCAAG AGATACACAT TGAGAACCAA GGAGATGACA 
ACTATAATGC ACGTTAACAG TCCGGTGCAA TACCTGAACT ACATCAACAA CAGGTTGATG 
CTGGTCGCGT GGAAGAACCG ATGTCTAATG TCGTATAAAC TGGGCCACGA GGAGACCTGG 
AAGACTGAAA TGCTACAGGC CGGTAACACG CACCTCGGCT CGCAAGAATC GCAGCTAGGA 
AGCGATTGGC AGAGTATGAA AATAAGGAAC GGCCACTCCG ACTTGTCGAC GTCGAGCAGC 
GAGGCCAGCG TGAGCTCCCG CGAGAACCGA CTGTTCCACG GCGGGGAGAT GAAGGAGCTG 
TACAAACAGA GCGACCTCGT CGACTGCTTC TGGGTCGCGG ACACCGGCCT CATCACGGTC 
GAGTCGAACG CGGCCATCAA ACTGTGGAAC AAAGACTGCA AAGTCTCAAC CATATTGAAC 
GCGAGGCAAA TGGATACTTA CATCACGTGC GCCGCTTTCC AGAACAACGT TCTCGTTATA 
TGCGATAGTG AAAACAAAGG ATTTCAGTCG TACGAACTAA AATTCGACAA CACAATAGAT 
TTGTGCCTCA TCCAAGAGTA CAAACTGAAC AATGTGGTGA CGTCATGCGA TATCACATCC 
GACGGCTACA TACTAGCGAT GGGACTAGAC TGTGGTAATG TTGTGCTTTG GAACGTAAAA 
GGGAAGCGTC AAATCAGTTT ACTCAAACAC CACAACTCGA GAGTGCAGGC GTGCTCGTTC 
TCGCCGGTAC CGGACCGACT GTACCGGAGC TCCGTGCACT CGCCCGGTGT GACGTCACCG 
GCGGACGCCG TCGCCGACGA CCAGCCTCCA CTGGTCCTGG TCACGATGGC TTCAGAGATA 
GTCTGGTGGA ACATCACGTA CGTCATACGG ATACGAGCGA GTAATAAGTC ATTGTGGAGA 
TGTAAAGAAG GGTCGAAACG CAAAGAAATA TTGGCGTGTA TAAAACTATC GGGTATAAAC 
GCGACGAGGT TGTGCCACGA CGAGAATTTC TCGTGCTTCG TGACCGTGGA CAATCCGGGC 
CACGTCCACA TCATGAACGT TATGAGGGAT CTTTGTACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apaf-1 ( XM_012697713.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012553167.1 Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg14515
Clone ID Related Accession (Same CDS sequence)	XM_012697713.1
Accession Version	XM_012697713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	apaf-1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581680.1) and transcript sequence (HQ179968.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 106 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCCCGGCT CCAAAAAATC CATGGCTGTA AAGTCGGCTG GAAAAGTGAT GGCTTCCGTC 
TTTTGGGATG CAGATGGGAT CTTGATGATA GATTACCTTC CTAAAGATGA GCGAGCCGCG 
ATCGAAATGG ATCTCAAGAA CAGGAAGCTG CTGCAGCACA ATCAGCAGGC TATCATCAAA 
GATCTAGATG TTCAGTACAT TCTAGACGAA CTTTACACGA AACAAGCTAT ATCTTCAGAG 
GTGTACGAAC ATATCGCTAA TCTGGTCATA GTTAAAAATC GAGTCGAAGG CGTCAGGTAT 
TTGATAGATT GCCTCCTAAG GTATGGCACG AATCAGGCCT ACGAAGCATT CGTTGATAGC 
CTTGCCAAAG ATTACAACTG GCTCTGGGAG AAGTTTGCTA CACAATCCAA CGGGCCAATG 
TTTAACGACA GCTTCGAGGA CAGCCTTAGC AAAGGCGACG TGCCAAGGCT TCCCGAACAC 
CATGTCAGGA GAAAGGCTGT GGAAACTGAA GTCCTATCCA AGATTAAAGA GTTGACCCGT 
CACAAGATAC TGGTGCTTCA CGGTATGCTT GGCAGCGGAA AGACCTGCGT CGCCATCAAC 
GTACTCCGGG ACAACCCCGA ACTCATAACG AACAATTTCA ATGATATTGT CTTTTGGATG 
AACTTTGCGA ATTGCAAGAC CGATGACGAC ATCATCGCTC AACAGAATAC GTTACTACGT 
AAAATATCAT CGATGTACAT TCAAAATTCT CACATGAACT CTTCGATATC AATGTCGAGT 
ATGGGCTCGA ATATCGACAG CCATTCGCTC TCCTACTACG ATCGCACGTG GAACGACTTC 
AGAGACAGAC TGAAGAACCT ATTCTCGGAG CCCCCGCTGA AGGAAGGTTT GCTCGTAATG 
GACGAAGTCA ACGATCGGAA ATGCGTCGAA GCGTTCGATA TCGGTTGCAA GATACTGATC 
ACGACCAGAG ATACGGACGT TGTGGCTAAT TATCACCCGC AAATCGTTAC GGTGGAGAAC 
AGTTTCACAG AGAGGGAGTC CCTAGAACTG TTTGCTTCTT GCTTAGACGA ACCTGTATCG 
AAACTTCCAT CGTATGCGAA AGAGCTCAAC GAATTATGCA AAGGAAGCCC CTTCCACGTG 
GCCCTGATCG GTGCCCAGCT CGCAGAAGAC AGGGAGAGAC TGAAACACGA TCGAAGTCAT 
TGGAAATATT ACTTGAAGAA ATTAAAAAAG AATTTCTTTT CTGTGTGGAA AACGAACAAC 
GATAACCCGA TGAAAACTAT ACAAGTGTGT ATAAACTCGT TGAACCCCAA CACGCTTGCG 
ATGTTCATGA AACTGGCAGT GCTTCCGGAC AGCGTCAAAG TCACCGCTCA AGTCTACAGC 
AAACTGTGGA ACGTAGACGT CAACGAAGTC GAAAACATAA CGAAGAAACT CAAGAGCAAG 
TCTCTGATCA TAAAGAACTA CGATAGAGAT CAAAAAAAGT ATTTATACGA AGTGCACGAT 
CTCATAATGA ACTATCTAAG GTCTTGTACG CACGAAGAGG AAATGAAGAA GCTACACGAC 
GAACTGTTGA ACAGTTACAG ATATGATAGC GAAACTGCAC CAACGGAGAT AGTCGATGAC 
GGATACATAG CCTTCTACAT AGGATTTCAC ATTTTGAAAA CGAAAAATTT GCACCAGAAA 
TGGAGTTTGT TCAGTAAGCT GTATTTAGAT TTGAGGTTTC TAGGGAATAA AGTCAGACTG 
ACCGGACCGG ACGATGTCAT GCTGGATCTG CAGAAATACG AAAATTATAT TGTGAGAGAT 
GAAATCGACA GAAACTTAGT GTACAGTATA AAAGCGTTTC TAAGCACGCA CGGCACAGAC 
CTGCACCACT ACCCCTGCAC GAACATTGTG CAAAGTATAA TACAACACGA AACGAAAGGA 
ATATTGTGTT GTAAGGCTTC GTTAATAGCA CGGGATAACT GCGTAAACAA CGAATTGTAT 
TTTGAGTTTA TGCACGAACA TGATGTAGAA GAAATTAAAC ATTCAACTAT CGACGTTAAG 
GAAGTTATCA AATCTGTTTG CTTCTTGAGA AATTATGTAA TTGTTGGGAC GTCCAATGGC 
TTGATAAAGT TCTTCAACAT AGAAACTAAT AAGCAGAAGA AGGAACTAAA GGGCACCGGT 
GCCGCCATTA AATGGATAGG CGTCGCGTGC ACTAAAGACA CGACTATTGT AGCCGCTCTG 
TCGACGGACG GATATCTCAA ACTTTGGTTC GTCGATGACT TCGATGATGA CGACAACGCT 
AATAATCTCG TCGAAGAAGA ACCGAACGAT GCCTACAACA ACTATCCCAG TCCGTTAACA 
TTTGAACCCA AACTGGGACC GATACTAAAC TGTCGTTGGA CGACCAGCGA AGACTTGTTA 
ATAACTCACA CGTCACAAAT GATTATACTG TACCGTCCCA AAGGCGCCGA GTATGACGTC 
ATAAGTGAGT TCGACAGGGA ACAGAAGCTG CTATGCTGCG TCCCCTGTAA CAACGACAAA 
TACATTGCTG TAGCTAGTTT CAACGCCGTA TACAGAGTGG ACTTGATCGA CATTAGAACT 
AAGCAGAAAG TTGTTTATTT CGAAGAAACA GGTGCCATAT TGGATATGCT TGTTGTTCCT 
GGTACCAATA AAATAATAAC GCTGAAACAA AACGAGATAA TGGCACACGA GTTCCGGGTA 
AAGCCTGCTT CGATAAACAG AGGCGGGACG GTTATCGATA GCGGTGCGGT CAAAGCGGAT 
GTCCGGTTCG CGGCGATGGC CGTCAACAAC TCGGGCTCGC TGCTGTTCGC GGCCACGGAC 
GATAGCCGGG TCGTTTGTGT CGACTTGAAA ACTAACTCAA GAGTTTTCGA TATCGAGAAC 
AGAAGGGGCA ATGTGGTGTC AATGGCGGTG AGCGAAGTGC TGATGTGGGA TGAATTCGAA 
CCGAGCTCCG ACGTACTTCT AACCGGTACT GATAGTACCG AAAATTCTGC CAAAGTTTGG 
TATTTAGACG CTTCATACGT GTCGCAGACT GCACAAAGAA AAGGAAAAGT TCGTCTAACA 
AGTAAATTCG ACGTGAGTTT CCTAAACGCG ACAACGCCAA CATCTCCGAG CGCCTCGCCT 
CTGAACAGCG GCGTGGAAAC AGTTCACAAC CCTCTAAAAT CACACCAGTC GTTCACGAAC 
AAGGACCCAG TAAAGAAACC GATTAAAACT ACCATGAGCT TGGACAGGAA TACATTGAAA 
CCGCTCAATC TCAAAGGAAT ATGCAACAAC AATGAAGGGC TCATACACCC ATTACTGGCT 
GTCGTCGATG ATATGAACAA TATACAGGTG ATGCGTGGTA AGAAAGTCCT AACCGAGATC 
TTAGAGAAAT CTGATGAGAA GATAACAGCC GTCAAGGTAT CGCCGTGCAA TCACTACATC 
ATGTACGGGC TCAAGTCGGG AAAAGTCAAG AGATACACAT TGAGAACCAA GGAGATGACA 
ACTATAATGC ACGTTAACAG TCCGGTGCAA TACCTGAACT ACATCAACAA CAGGTTGATG 
CTGGTCGCGT GGAAGAACCG ATGTCTAATG TCGTATAAAC TGGGCCACGA GGAGACCTGG 
AAGACTGAAA TGCTACAGGC CGGTAACACG CACCTCGGCT CGCAAGAATC GCAGCTAGGA 
AGCGATTGGC AGAGTATGAA AATAAGGAAC GGCCACTCCG ACTTGTCGAC GTCGAGCAGC 
GAGGCCAGCG TGAGCTCCCG CGAGAACCGA CTGTTCCACG GCGGGGAGAT GAAGGAGCTG 
TACAAACAGA GCGACCTCGT CGACTGCTTC TGGGTCGCGG ACACCGGCCT CATCACGGTC 
GAGTCGAACG CGGCCATCAA ACTGTGGAAC AAAGACTGCA AAGTCTCAAC CATATTGAAC 
GCGAGGCAAA TGGATACTTA CATCACGTGC GCCGCTTTCC AGAACAACGT TCTCGTTATA 
TGCGATAGTG AAAACAAAGG ATTTCAGTCG TACGAACTAA AATTCGACAA CACAATAGAT 
TTGTGCCTCA TCCAAGAGTA CAAACTGAAC AATGTGGTGA CGTCATGCGA TATCACATCC 
GACGGCTACA TACTAGCGAT GGGACTAGAC TGTGGTAATG TTGTGCTTTG GAACGTAAAA 
GGGAAGCGTC AAATCAGTTT ACTCAAACAC CACAACTCGA GAGTGCAGGC GTGCTCGTTC 
TCGCCGGTAC CGGACCGACT GTACCGGAGC TCCGTGCACT CGCCCGGTGT GACGTCACCG 
GCGGACGCCG TCGCCGACGA CCAGCCTCCA CTGGTCCTGG TCACGATGGC TTCAGAGATA 
GTCTGGTGGA ACATCACGTA CGTCATACGG ATACGAGCGA GTAATAAGTC ATTGTGGAGG 
AGCGGACTCA ATGTTATATC GCCAATTGGA ACGCCATTAG AGAATCGAGC TGAGAATCAC 
CAGACGACAG AAAATTCGGG CAACGTTAAT ACCAATTTCT TCTTCAGAGA TATGTTCGCA 
GATAGACATT GTTGGAAGAA CATTTGGAAA GGAAAAACAT GTAAAGAAGG GTCGAAACGC 
AAAGAAATAT TGGCGTGTAT AAAACTATCG GGTATAAACG CGACGAGGTT GTGCCACGAC 
GAGAATTTCT CGTGCTTCGT GACCGTGGAC AATCCGGGCC ACGTCCACAT CATGAACGTT 
ATGAGGGATC TTTGTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012553167.1
CDS	1..4701
Translation	MPGSKKSMAVKSAGKVMASVFWDADGILMIDYLPKDERAAIEMDLKNRKLLQHNQQAIIKDLDVQYILDELYTKQAISSEVYEHIANLVIVKNRVEGVRYLIDCLLRYGTNQAYEAFVDSLAKDYNWLWEKFATQSNGPMFNDSFEDSLSKGDVPRLPEHHVRRKAVETEVLSKIKELTRHKILVLHGMLGSGKTCVAINVLRDNPELITNNFNDIVFWMNFANCKTDDDIIAQQNTLLRKISSMYIQNSHMNSSISMSSMGSNIDSHSLSYYDRTWNDFRDRLKNLFSEPPLKEGLLVMDEVNDRKCVEAFDIGCKILITTRDTDVVANYHPQIVTVENSFTERESLELFASCLDEPVSKLPSYAKELNELCKGSPFHVALIGAQLAEDRERLKHDRSHWKYYLKKLKKNFFSVWKTNNDNPMKTIQVCINSLNPNTLAMFMKLAVLPDSVKVTAQVYSKLWNVDVNEVENITKKLKSKSLIIKNYDRDQKKYLYEVHDLIMNYLRSCTHEEEMKKLHDELLNSYRYDSETAPTEIVDDGYIAFYIGFHILKTKNLHQKWSLFSKLYLDLRFLGNKVRLTGPDDVMLDLQKYENYIVRDEIDRNLVYSIKAFLSTHGTDLHHYPCTNIVQSIIQHETKGILCCKASLIARDNCVNNELYFEFMHEHDVEEIKHSTIDVKEVIKSVCFLRNYVIVGTSNGLIKFFNIETNKQKKELKGTGAAIKWIGVACTKDTTIVAALSTDGYLKLWFVDDFDDDDNANNLVEEEPNDAYNNYPSPLTFEPKLGPILNCRWTTSEDLLITHTSQMIILYRPKGAEYDVISEFDREQKLLCCVPCNNDKYIAVASFNAVYRVDLIDIRTKQKVVYFEETGAILDMLVVPGTNKIITLKQNEIMAHEFRVKPASINRGGTVIDSGAVKADVRFAAMAVNNSGSLLFAATDDSRVVCVDLKTNSRVFDIENRRGNVVSMAVSEVLMWDEFEPSSDVLLTGTDSTENSAKVWYLDASYVSQTAQRKGKVRLTSKFDVSFLNATTPTSPSASPLNSGVETVHNPLKSHQSFTNKDPVKKPIKTTMSLDRNTLKPLNLKGICNNNEGLIHPLLAVVDDMNNIQVMRGKKVLTEILEKSDEKITAVKVSPCNHYIMYGLKSGKVKRYTLRTKEMTTIMHVNSPVQYLNYINNRLMLVAWKNRCLMSYKLGHEETWKTEMLQAGNTHLGSQESQLGSDWQSMKIRNGHSDLSTSSSEASVSSRENRLFHGGEMKELYKQSDLVDCFWVADTGLITVESNAAIKLWNKDCKVSTILNARQMDTYITCAAFQNNVLVICDSENKGFQSYELKFDNTIDLCLIQEYKLNNVVTSCDITSDGYILAMGLDCGNVVLWNVKGKRQISLLKHHNSRVQACSFSPVPDRLYRSSVHSPGVTSPADAVADDQPPLVLVTMASEIVWWNITYVIRIRASNKSLWRSGLNVISPIGTPLENRAENHQTTENSGNVNTNFFFRDMFADRHCWKNIWKGKTCKEGSKRKEILACIKLSGINATRLCHDENFSCFVTVDNPGHVHIMNVMRDLCT

Target ORF information:

RefSeq Version	XM_012697713.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012697713.1

ORF Insert Sequence:

ATGCCCGGCT CCAAAAAATC CATGGCTGTA AAGTCGGCTG GAAAAGTGAT GGCTTCCGTC 
TTTTGGGATG CAGATGGGAT CTTGATGATA GATTACCTTC CTAAAGATGA GCGAGCCGCG 
ATCGAAATGG ATCTCAAGAA CAGGAAGCTG CTGCAGCACA ATCAGCAGGC TATCATCAAA 
GATCTAGATG TTCAGTACAT TCTAGACGAA CTTTACACGA AACAAGCTAT ATCTTCAGAG 
GTGTACGAAC ATATCGCTAA TCTGGTCATA GTTAAAAATC GAGTCGAAGG CGTCAGGTAT 
TTGATAGATT GCCTCCTAAG GTATGGCACG AATCAGGCCT ACGAAGCATT CGTTGATAGC 
CTTGCCAAAG ATTACAACTG GCTCTGGGAG AAGTTTGCTA CACAATCCAA CGGGCCAATG 
TTTAACGACA GCTTCGAGGA CAGCCTTAGC AAAGGCGACG TGCCAAGGCT TCCCGAACAC 
CATGTCAGGA GAAAGGCTGT GGAAACTGAA GTCCTATCCA AGATTAAAGA GTTGACCCGT 
CACAAGATAC TGGTGCTTCA CGGTATGCTT GGCAGCGGAA AGACCTGCGT CGCCATCAAC 
GTACTCCGGG ACAACCCCGA ACTCATAACG AACAATTTCA ATGATATTGT CTTTTGGATG 
AACTTTGCGA ATTGCAAGAC CGATGACGAC ATCATCGCTC AACAGAATAC GTTACTACGT 
AAAATATCAT CGATGTACAT TCAAAATTCT CACATGAACT CTTCGATATC AATGTCGAGT 
ATGGGCTCGA ATATCGACAG CCATTCGCTC TCCTACTACG ATCGCACGTG GAACGACTTC 
AGAGACAGAC TGAAGAACCT ATTCTCGGAG CCCCCGCTGA AGGAAGGTTT GCTCGTAATG 
GACGAAGTCA ACGATCGGAA ATGCGTCGAA GCGTTCGATA TCGGTTGCAA GATACTGATC 
ACGACCAGAG ATACGGACGT TGTGGCTAAT TATCACCCGC AAATCGTTAC GGTGGAGAAC 
AGTTTCACAG AGAGGGAGTC CCTAGAACTG TTTGCTTCTT GCTTAGACGA ACCTGTATCG 
AAACTTCCAT CGTATGCGAA AGAGCTCAAC GAATTATGCA AAGGAAGCCC CTTCCACGTG 
GCCCTGATCG GTGCCCAGCT CGCAGAAGAC AGGGAGAGAC TGAAACACGA TCGAAGTCAT 
TGGAAATATT ACTTGAAGAA ATTAAAAAAG AATTTCTTTT CTGTGTGGAA AACGAACAAC 
GATAACCCGA TGAAAACTAT ACAAGTGTGT ATAAACTCGT TGAACCCCAA CACGCTTGCG 
ATGTTCATGA AACTGGCAGT GCTTCCGGAC AGCGTCAAAG TCACCGCTCA AGTCTACAGC 
AAACTGTGGA ACGTAGACGT CAACGAAGTC GAAAACATAA CGAAGAAACT CAAGAGCAAG 
TCTCTGATCA TAAAGAACTA CGATAGAGAT CAAAAAAAGT ATTTATACGA AGTGCACGAT 
CTCATAATGA ACTATCTAAG GTCTTGTACG CACGAAGAGG AAATGAAGAA GCTACACGAC 
GAACTGTTGA ACAGTTACAG ATATGATAGC GAAACTGCAC CAACGGAGAT AGTCGATGAC 
GGATACATAG CCTTCTACAT AGGATTTCAC ATTTTGAAAA CGAAAAATTT GCACCAGAAA 
TGGAGTTTGT TCAGTAAGCT GTATTTAGAT TTGAGGTTTC TAGGGAATAA AGTCAGACTG 
ACCGGACCGG ACGATGTCAT GCTGGATCTG CAGAAATACG AAAATTATAT TGTGAGAGAT 
GAAATCGACA GAAACTTAGT GTACAGTATA AAAGCGTTTC TAAGCACGCA CGGCACAGAC 
CTGCACCACT ACCCCTGCAC GAACATTGTG CAAAGTATAA TACAACACGA AACGAAAGGA 
ATATTGTGTT GTAAGGCTTC GTTAATAGCA CGGGATAACT GCGTAAACAA CGAATTGTAT 
TTTGAGTTTA TGCACGAACA TGATGTAGAA GAAATTAAAC ATTCAACTAT CGACGTTAAG 
GAAGTTATCA AATCTGTTTG CTTCTTGAGA AATTATGTAA TTGTTGGGAC GTCCAATGGC 
TTGATAAAGT TCTTCAACAT AGAAACTAAT AAGCAGAAGA AGGAACTAAA GGGCACCGGT 
GCCGCCATTA AATGGATAGG CGTCGCGTGC ACTAAAGACA CGACTATTGT AGCCGCTCTG 
TCGACGGACG GATATCTCAA ACTTTGGTTC GTCGATGACT TCGATGATGA CGACAACGCT 
AATAATCTCG TCGAAGAAGA ACCGAACGAT GCCTACAACA ACTATCCCAG TCCGTTAACA 
TTTGAACCCA AACTGGGACC GATACTAAAC TGTCGTTGGA CGACCAGCGA AGACTTGTTA 
ATAACTCACA CGTCACAAAT GATTATACTG TACCGTCCCA AAGGCGCCGA GTATGACGTC 
ATAAGTGAGT TCGACAGGGA ACAGAAGCTG CTATGCTGCG TCCCCTGTAA CAACGACAAA 
TACATTGCTG TAGCTAGTTT CAACGCCGTA TACAGAGTGG ACTTGATCGA CATTAGAACT 
AAGCAGAAAG TTGTTTATTT CGAAGAAACA GGTGCCATAT TGGATATGCT TGTTGTTCCT 
GGTACCAATA AAATAATAAC GCTGAAACAA AACGAGATAA TGGCACACGA GTTCCGGGTA 
AAGCCTGCTT CGATAAACAG AGGCGGGACG GTTATCGATA GCGGTGCGGT CAAAGCGGAT 
GTCCGGTTCG CGGCGATGGC CGTCAACAAC TCGGGCTCGC TGCTGTTCGC GGCCACGGAC 
GATAGCCGGG TCGTTTGTGT CGACTTGAAA ACTAACTCAA GAGTTTTCGA TATCGAGAAC 
AGAAGGGGCA ATGTGGTGTC AATGGCGGTG AGCGAAGTGC TGATGTGGGA TGAATTCGAA 
CCGAGCTCCG ACGTACTTCT AACCGGTACT GATAGTACCG AAAATTCTGC CAAAGTTTGG 
TATTTAGACG CTTCATACGT GTCGCAGACT GCACAAAGAA AAGGAAAAGT TCGTCTAACA 
AGTAAATTCG ACGTGAGTTT CCTAAACGCG ACAACGCCAA CATCTCCGAG CGCCTCGCCT 
CTGAACAGCG GCGTGGAAAC AGTTCACAAC CCTCTAAAAT CACACCAGTC GTTCACGAAC 
AAGGACCCAG TAAAGAAACC GATTAAAACT ACCATGAGCT TGGACAGGAA TACATTGAAA 
CCGCTCAATC TCAAAGGAAT ATGCAACAAC AATGAAGGGC TCATACACCC ATTACTGGCT 
GTCGTCGATG ATATGAACAA TATACAGGTG ATGCGTGGTA AGAAAGTCCT AACCGAGATC 
TTAGAGAAAT CTGATGAGAA GATAACAGCC GTCAAGGTAT CGCCGTGCAA TCACTACATC 
ATGTACGGGC TCAAGTCGGG AAAAGTCAAG AGATACACAT TGAGAACCAA GGAGATGACA 
ACTATAATGC ACGTTAACAG TCCGGTGCAA TACCTGAACT ACATCAACAA CAGGTTGATG 
CTGGTCGCGT GGAAGAACCG ATGTCTAATG TCGTATAAAC TGGGCCACGA GGAGACCTGG 
AAGACTGAAA TGCTACAGGC CGGTAACACG CACCTCGGCT CGCAAGAATC GCAGCTAGGA 
AGCGATTGGC AGAGTATGAA AATAAGGAAC GGCCACTCCG ACTTGTCGAC GTCGAGCAGC 
GAGGCCAGCG TGAGCTCCCG CGAGAACCGA CTGTTCCACG GCGGGGAGAT GAAGGAGCTG 
TACAAACAGA GCGACCTCGT CGACTGCTTC TGGGTCGCGG ACACCGGCCT CATCACGGTC 
GAGTCGAACG CGGCCATCAA ACTGTGGAAC AAAGACTGCA AAGTCTCAAC CATATTGAAC 
GCGAGGCAAA TGGATACTTA CATCACGTGC GCCGCTTTCC AGAACAACGT TCTCGTTATA 
TGCGATAGTG AAAACAAAGG ATTTCAGTCG TACGAACTAA AATTCGACAA CACAATAGAT 
TTGTGCCTCA TCCAAGAGTA CAAACTGAAC AATGTGGTGA CGTCATGCGA TATCACATCC 
GACGGCTACA TACTAGCGAT GGGACTAGAC TGTGGTAATG TTGTGCTTTG GAACGTAAAA 
GGGAAGCGTC AAATCAGTTT ACTCAAACAC CACAACTCGA GAGTGCAGGC GTGCTCGTTC 
TCGCCGGTAC CGGACCGACT GTACCGGAGC TCCGTGCACT CGCCCGGTGT GACGTCACCG 
GCGGACGCCG TCGCCGACGA CCAGCCTCCA CTGGTCCTGG TCACGATGGC TTCAGAGATA 
GTCTGGTGGA ACATCACGTA CGTCATACGG ATACGAGCGA GTAATAAGTC ATTGTGGAGG 
AGCGGACTCA ATGTTATATC GCCAATTGGA ACGCCATTAG AGAATCGAGC TGAGAATCAC 
CAGACGACAG AAAATTCGGG CAACGTTAAT ACCAATTTCT TCTTCAGAGA TATGTTCGCA 
GATAGACATT GTTGGAAGAA CATTTGGAAA GGAAAAACAT GTAAAGAAGG GTCGAAACGC 
AAAGAAATAT TGGCGTGTAT AAAACTATCG GGTATAAACG CGACGAGGTT GTGCCACGAC 
GAGAATTTCT CGTGCTTCGT GACCGTGGAC AATCCGGGCC ACGTCCACAT CATGAACGTT 
ATGAGGGATC TTTGTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apaf-1 ( XM_012697713.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012553167.2 Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.

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CloneID	OBg22410
Clone ID Related Accession (Same CDS sequence)	XM_012697713.2 , XM_021353580.1
Accession Version	XM_012697713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	apaf-1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012697713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTCA AGAACAGGAA GCTGCTGCAG CACAATCAGC AGGCTATCAT CAAAGATCTA 
GATGTTCAGT ACATTCTAGA CGAACTTTAC ACGAAACAAG CTATATCTTC AGAGGTGTAC 
GAACATATCG CTAATCTGGT CATAGTTAAA AATCGAGTCG AAGGCGTCAG GTATTTGATA 
GATTGCCTCC TAAGGTATGG CACGAATCAG GCCTACGAAG CATTCGTTGA TAGCCTTGCC 
AAAGATTACA ACTGGCTCTG GGAGAAGTTT GCTACACAAT CCAACGGGCC AATGTTTAAC 
GACAGCTTCG AGGACAGCCT TAGCAAAGGC GACGTGCCAA GGCTTCCCGA ACACCATGTC 
AGGAGAAAGG CTGTGGAAAC TGAAGTCCTA TCCAAGATTA AAGAGTTGAC CCGTCACAAG 
ATACTGGTGC TTCACGGTAT GCTTGGCAGC GGAAAGACCT GCGTCGCCAT CAACGTACTC 
CGGGACAACC CCGAACTCAT AACGAACAAT TTCAATGATA TTGTCTTTTG GATGAACTTT 
GCGAATTGCA AGACCGATGA CGACATCATC GCTCAACAGA ATACGTTACT ACGTAAAATA 
TCATCGATGT ACATTCAAAA TTCTCACATG AACTCTTCGA TATCAATGTC GAGTATGGGC 
TCGAATATCG ACAGCCATTC GCTCTCCTAC TACGATCGCA CGTGGAACGA CTTCAGAGAC 
AGACTGAAGA ACCTATTCTC GGAGCCCCCG CTGAAGGAAG GTTTGCTCGT AATGGACGAA 
GTCAACGATC GGAAATGCGT CGAAGCGTTC GATATCGGTT GCAAGATACT GATCACGACC 
AGAGATACGG ACGTTGTGGC TAATTATCAC CCGCAAATCG TTACGGTGGA GAACAGTTTC 
ACAGAGAGGG AGTCCCTAGA ACTGTTTGCT TCTTGCTTAG ACGAACCTGT ATCGAAACTT 
CCATCGTATG CGAAAGAGCT CAACGAATTA TGCAAAGGAA GCCCCTTCCA CGTGGCCCTG 
ATCGGTGCCC AGCTCGCAGA AGACAGGGAG AGACTGAAAC ACGATCGAAG TCATTGGAAA 
TATTACTTGA AGAAATTAAA AAAGAATTTC TTTTCTGTGT GGAAAACGAA CAACGATAAC 
CCGATGAAAA CTATACAAGT GTGTATAAAC TCGTTGAACC CCAACACGCT TGCGATGTTC 
ATGAAACTGG CAGTGCTTCC GGACAGCGTC AAAGTCACCG CTCAAGTCTA CAGCAAACTG 
TGGAACGTAG ACGTCAACGA AGTCGAAAAC ATAACGAAGA AACTCAAGAG CAAGTCTCTG 
ATCATAAAGA ACTACGATAG AGATCAAAAA AAGTATTTAT ACGAAGTGCA CGATCTCATA 
ATGAACTATC TAAGGTCTTG TACGCACGAA GAGGAAATGA AGAAGCTACA CGACGAACTG 
TTGAACAGTT ACAGATATGA TAGCGAAACT GCACCAACGG AGATAGTCGA TGACGGATAC 
ATAGCCTTCT ACATAGGATT TCACATTTTG AAAACGAAAA ATTTGCACCA GAAATGGAGT 
TTGTTCAGTA AGCTGTATTT AGATTTGAGG TTTCTAGGGA ATAAAGTCAG ACTGACCGGA 
CCGGACGATG TCATGCTGGA TCTGCAGAAA TACGAAAATT ATATTGTGAG AGATGAAATC 
GACAGAAACT TAGTGTACAG TATAAAAGCG TTTCTAAGCA CGCACGGCAC AGACCTGCAC 
CACTACCCCT GCACGAACAT TGTGCAAAGT ATAATACAAC ACGAAACGAA AGGAATATTG 
TGTTGTAAGG CTTCGTTAAT AGCACGGGAT AACTGCGTAA ACAACGAATT GTATTTTGAG 
TTTATGCACG AACATGATGT AGAAGAAATT AAACATTCAA CTATCGACGT TAAGGAAGTT 
ATCAAATCTG TTTGCTTCTT GAGAAATTAT GTAATTGTTG GGACGTCCAA TGGCTTGATA 
AAGTTCTTCA ACATAGAAAC TAATAAGCAG AAGAAGGAAC TAAAGGGCAC CGGTGCCGCC 
ATTAAATGGA TAGGCGTCGC GTGCACTAAA GACACGACTA TTGTAGCCGC TCTGTCGACG 
GACGGATATC TCAAACTTTG GTTCGTCGAT GACTTCGATG ATGACGACAA CGCTAATAAT 
CTCGTCGAAG AAGAACCGAA CGATGCCTAC AACAACTATC CCAGTCCGTT AACATTTGAA 
CCCAAACTGG GACCGATACT AAACTGTCGT TGGACGACCA GCGAAGACTT GTTAATAACT 
CACACGTCAC AAATGATTAT ACTGTACCGT CCCAAAGGCG CCGAGTATGA CGTCATAAGT 
GAGTTCGACA GGGAACAGAA GCTGCTATGC TGCGTCCCCT GTAACAACGA CAAATACATT 
GCTGTAGCTA GTTTCAACGC CGTATACAGA GTGGACTTGA TCGACATTAG AACTAAGCAG 
AAAGTTGTTT ATTTCGAAGA AACAGGTGCC ATATTGGATA TGCTTGTTGT TCCTGGTACC 
AATAAAATAA TAACGCTGAA ACAAAACGAG ATAATGGCAC ACGAGTTCCG GGTAAAGCCT 
GCTTCGATAA ACAGAGGCGG GACGGTTATC GATAGCGGTG CGGTCAAAGC GGATGTCCGG 
TTCGCGGCGA TGGCCGTCAA CAACTCGGGC TCGCTGCTGT TCGCGGCCAC GGACGATAGC 
CGGGTCGTTT GTGTCGACTT GAAAACTAAC TCAAGAGTTT TCGATATCGA GAACAGAAGG 
GGCAATGTGG TGTCAATGGC GGTGAGCGAA GTGCTGATGT GGGATGAATT CGAACCGAGC 
TCCGACGTAC TTCTAACCGG TACTGATAGT ACCGAAAATT CTGCCAAAGT TTGGTATTTA 
GACGCTTCAT ACGTGTCGCA GACTGCACAA AGAAAAGGAA AAGTTCGTCT AACAAGTAAA 
TTCGACGTGA GTTTCCTAAA CGCGACAACG CCAACATCTC CGAGCGCCTC GCCTCTGAAC 
AGCGGCGTGG AAACAGTTCA CAACCCTCTA AAATCACACC AGTCGTTCAC GAACAAGGAC 
CCAGTAAAGA AACCGATTAA AACTACCATG AGCTTGGACA GGAATACATT GAAACCGCTC 
AATCTCAAAG GAATATGCAA CAACAATGAA GGGCTCATAC ACCCATTACT GGCTGTCGTC 
GATGATATGA ACAATATACA GGTGATGCGT GGTAAGAAAG TCCTAACCGA GATCTTAGAG 
AAATCTGATG AGAAGATAAC AGCCGTCAAG GTATCGCCGT GCAATCACTA CATCATGTAC 
GGGCTCAAGT CGGGAAAAGT CAAGAGATAC ACATTGAGAA CCAAGGAGAT GACAACTATA 
ATGCACGTTA ACAGTCCGGT GCAATACCTG AACTACATCA ACAACAGGTT GATGCTGGTC 
GCGTGGAAGA ACCGATGTCT AATGTCGTAT AAACTGGGCC ACGAGGAGAC CTGGAAGACT 
GAAATGCTAC AGGCCGGTAA CACGCACCTC GGCTCGCAAG AATCGCAGCT AGGAAGCGAT 
TGGCAGAGTA TGAAAATAAG GAACGGCCAC TCCGACTTGT CGACGTCGAG CAGCGAGGCC 
AGCGTGAGCT CCCGCGAGAA CCGACTGTTC CACGGCGGGG AGATGAAGGA GCTGTACAAA 
CAGAGCGACC TCGTCGACTG CTTCTGGGTC GCGGACACCG GCCTCATCAC GGTCGAGTCG 
AACGCGGCCA TCAAACTGTG GAACAAAGAC TGCAAAGTCT CAACCATATT GAACGCGAGG 
CAAATGGATA CTTACATCAC GTGCGCCGCT TTCCAGAACA ACGTTCTCGT TATATGCGAT 
AGTGAAAACA AAGGATTTCA GTCGTACGAA CTAAAATTCG ACAACACAAT AGATTTGTGC 
CTCATCCAAG AGTACAAACT GAACAATGTG GTGACGTCAT GCGATATCAC ATCCGACGGC 
TACATACTAG CGATGGGACT AGACTGTGGT AATGTTGTGC TTTGGAACGT AAAAGGGAAG 
CGTCAAATCA GTTTACTCAA ACACCACAAC TCGAGAGTGC AGGCGTGCTC GTTCTCGCCG 
GTACCGGACC GACTGTACCG GAGCTCCGTG CACTCGCCCG GTGTGACGTC ACCGGCGGAC 
GCCGTCGCCG ACGACCAGCC TCCACTGGTC CTGGTCACGA TGGCTTCAGA GATAGTCTGG 
TGGAACATCA CGTACGTCAT ACGGATACGA GCGAGTAATA AGTCATTGTG GAGGAGCGGA 
CTCAATGTTA TATCGCCAAT TGGAACGCCA TTAGAGAATC GAGCTGAGAA TCACCAGACG 
ACAGAAAATT CGGGCAACGT TAATACCAAT TTCTTCTTCA GAGATATGTT CGCAGATAGA 
CATTGTTGGA AGAACATTTG GAAAGGAAAA ACATGTAAAG AAGGGTCGAA ACGCAAAGAA 
ATATTGGCGT GTATAAAACT ATCGGGTATA AACGCGACGA GGTTGTGCCA CGACGAGAAT 
TTCTCGTGCT TCGTGACCGT GGACAATCCG GGCCACGTCC ACATCATGAA CGTTATGAGG 
GATCTTTGTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012553167.2
CDS	233..4807
Translation	MDLKNRKLLQHNQQAIIKDLDVQYILDELYTKQAISSEVYEHIANLVIVKNRVEGVRYLIDCLLRYGTNQAYEAFVDSLAKDYNWLWEKFATQSNGPMFNDSFEDSLSKGDVPRLPEHHVRRKAVETEVLSKIKELTRHKILVLHGMLGSGKTCVAINVLRDNPELITNNFNDIVFWMNFANCKTDDDIIAQQNTLLRKISSMYIQNSHMNSSISMSSMGSNIDSHSLSYYDRTWNDFRDRLKNLFSEPPLKEGLLVMDEVNDRKCVEAFDIGCKILITTRDTDVVANYHPQIVTVENSFTERESLELFASCLDEPVSKLPSYAKELNELCKGSPFHVALIGAQLAEDRERLKHDRSHWKYYLKKLKKNFFSVWKTNNDNPMKTIQVCINSLNPNTLAMFMKLAVLPDSVKVTAQVYSKLWNVDVNEVENITKKLKSKSLIIKNYDRDQKKYLYEVHDLIMNYLRSCTHEEEMKKLHDELLNSYRYDSETAPTEIVDDGYIAFYIGFHILKTKNLHQKWSLFSKLYLDLRFLGNKVRLTGPDDVMLDLQKYENYIVRDEIDRNLVYSIKAFLSTHGTDLHHYPCTNIVQSIIQHETKGILCCKASLIARDNCVNNELYFEFMHEHDVEEIKHSTIDVKEVIKSVCFLRNYVIVGTSNGLIKFFNIETNKQKKELKGTGAAIKWIGVACTKDTTIVAALSTDGYLKLWFVDDFDDDDNANNLVEEEPNDAYNNYPSPLTFEPKLGPILNCRWTTSEDLLITHTSQMIILYRPKGAEYDVISEFDREQKLLCCVPCNNDKYIAVASFNAVYRVDLIDIRTKQKVVYFEETGAILDMLVVPGTNKIITLKQNEIMAHEFRVKPASINRGGTVIDSGAVKADVRFAAMAVNNSGSLLFAATDDSRVVCVDLKTNSRVFDIENRRGNVVSMAVSEVLMWDEFEPSSDVLLTGTDSTENSAKVWYLDASYVSQTAQRKGKVRLTSKFDVSFLNATTPTSPSASPLNSGVETVHNPLKSHQSFTNKDPVKKPIKTTMSLDRNTLKPLNLKGICNNNEGLIHPLLAVVDDMNNIQVMRGKKVLTEILEKSDEKITAVKVSPCNHYIMYGLKSGKVKRYTLRTKEMTTIMHVNSPVQYLNYINNRLMLVAWKNRCLMSYKLGHEETWKTEMLQAGNTHLGSQESQLGSDWQSMKIRNGHSDLSTSSSEASVSSRENRLFHGGEMKELYKQSDLVDCFWVADTGLITVESNAAIKLWNKDCKVSTILNARQMDTYITCAAFQNNVLVICDSENKGFQSYELKFDNTIDLCLIQEYKLNNVVTSCDITSDGYILAMGLDCGNVVLWNVKGKRQISLLKHHNSRVQACSFSPVPDRLYRSSVHSPGVTSPADAVADDQPPLVLVTMASEIVWWNITYVIRIRASNKSLWRSGLNVISPIGTPLENRAENHQTTENSGNVNTNFFFRDMFADRHCWKNIWKGKTCKEGSKRKEILACIKLSGINATRLCHDENFSCFVTVDNPGHVHIMNVMRDLCT

Target ORF information:

RefSeq Version	XM_012697713.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012697713.2

ORF Insert Sequence:

ATGGATCTCA AGAACAGGAA GCTGCTGCAG CACAATCAGC AGGCTATCAT CAAAGATCTA 
GATGTTCAGT ACATTCTAGA CGAACTTTAC ACGAAACAAG CTATATCTTC AGAGGTGTAC 
GAACATATCG CTAATCTGGT CATAGTTAAA AATCGAGTCG AAGGCGTCAG GTATTTGATA 
GATTGCCTCC TAAGGTATGG CACGAATCAG GCCTACGAAG CATTCGTTGA TAGCCTTGCC 
AAAGATTACA ACTGGCTCTG GGAGAAGTTT GCTACACAAT CCAACGGGCC AATGTTTAAC 
GACAGCTTCG AGGACAGCCT TAGCAAAGGC GACGTGCCAA GGCTTCCCGA ACACCATGTC 
AGGAGAAAGG CTGTGGAAAC TGAAGTCCTA TCCAAGATTA AAGAGTTGAC CCGTCACAAG 
ATACTGGTGC TTCACGGTAT GCTTGGCAGC GGAAAGACCT GCGTCGCCAT CAACGTACTC 
CGGGACAACC CCGAACTCAT AACGAACAAT TTCAATGATA TTGTCTTTTG GATGAACTTT 
GCGAATTGCA AGACCGATGA CGACATCATC GCTCAACAGA ATACGTTACT ACGTAAAATA 
TCATCGATGT ACATTCAAAA TTCTCACATG AACTCTTCGA TATCAATGTC GAGTATGGGC 
TCGAATATCG ACAGCCATTC GCTCTCCTAC TACGATCGCA CGTGGAACGA CTTCAGAGAC 
AGACTGAAGA ACCTATTCTC GGAGCCCCCG CTGAAGGAAG GTTTGCTCGT AATGGACGAA 
GTCAACGATC GGAAATGCGT CGAAGCGTTC GATATCGGTT GCAAGATACT GATCACGACC 
AGAGATACGG ACGTTGTGGC TAATTATCAC CCGCAAATCG TTACGGTGGA GAACAGTTTC 
ACAGAGAGGG AGTCCCTAGA ACTGTTTGCT TCTTGCTTAG ACGAACCTGT ATCGAAACTT 
CCATCGTATG CGAAAGAGCT CAACGAATTA TGCAAAGGAA GCCCCTTCCA CGTGGCCCTG 
ATCGGTGCCC AGCTCGCAGA AGACAGGGAG AGACTGAAAC ACGATCGAAG TCATTGGAAA 
TATTACTTGA AGAAATTAAA AAAGAATTTC TTTTCTGTGT GGAAAACGAA CAACGATAAC 
CCGATGAAAA CTATACAAGT GTGTATAAAC TCGTTGAACC CCAACACGCT TGCGATGTTC 
ATGAAACTGG CAGTGCTTCC GGACAGCGTC AAAGTCACCG CTCAAGTCTA CAGCAAACTG 
TGGAACGTAG ACGTCAACGA AGTCGAAAAC ATAACGAAGA AACTCAAGAG CAAGTCTCTG 
ATCATAAAGA ACTACGATAG AGATCAAAAA AAGTATTTAT ACGAAGTGCA CGATCTCATA 
ATGAACTATC TAAGGTCTTG TACGCACGAA GAGGAAATGA AGAAGCTACA CGACGAACTG 
TTGAACAGTT ACAGATATGA TAGCGAAACT GCACCAACGG AGATAGTCGA TGACGGATAC 
ATAGCCTTCT ACATAGGATT TCACATTTTG AAAACGAAAA ATTTGCACCA GAAATGGAGT 
TTGTTCAGTA AGCTGTATTT AGATTTGAGG TTTCTAGGGA ATAAAGTCAG ACTGACCGGA 
CCGGACGATG TCATGCTGGA TCTGCAGAAA TACGAAAATT ATATTGTGAG AGATGAAATC 
GACAGAAACT TAGTGTACAG TATAAAAGCG TTTCTAAGCA CGCACGGCAC AGACCTGCAC 
CACTACCCCT GCACGAACAT TGTGCAAAGT ATAATACAAC ACGAAACGAA AGGAATATTG 
TGTTGTAAGG CTTCGTTAAT AGCACGGGAT AACTGCGTAA ACAACGAATT GTATTTTGAG 
TTTATGCACG AACATGATGT AGAAGAAATT AAACATTCAA CTATCGACGT TAAGGAAGTT 
ATCAAATCTG TTTGCTTCTT GAGAAATTAT GTAATTGTTG GGACGTCCAA TGGCTTGATA 
AAGTTCTTCA ACATAGAAAC TAATAAGCAG AAGAAGGAAC TAAAGGGCAC CGGTGCCGCC 
ATTAAATGGA TAGGCGTCGC GTGCACTAAA GACACGACTA TTGTAGCCGC TCTGTCGACG 
GACGGATATC TCAAACTTTG GTTCGTCGAT GACTTCGATG ATGACGACAA CGCTAATAAT 
CTCGTCGAAG AAGAACCGAA CGATGCCTAC AACAACTATC CCAGTCCGTT AACATTTGAA 
CCCAAACTGG GACCGATACT AAACTGTCGT TGGACGACCA GCGAAGACTT GTTAATAACT 
CACACGTCAC AAATGATTAT ACTGTACCGT CCCAAAGGCG CCGAGTATGA CGTCATAAGT 
GAGTTCGACA GGGAACAGAA GCTGCTATGC TGCGTCCCCT GTAACAACGA CAAATACATT 
GCTGTAGCTA GTTTCAACGC CGTATACAGA GTGGACTTGA TCGACATTAG AACTAAGCAG 
AAAGTTGTTT ATTTCGAAGA AACAGGTGCC ATATTGGATA TGCTTGTTGT TCCTGGTACC 
AATAAAATAA TAACGCTGAA ACAAAACGAG ATAATGGCAC ACGAGTTCCG GGTAAAGCCT 
GCTTCGATAA ACAGAGGCGG GACGGTTATC GATAGCGGTG CGGTCAAAGC GGATGTCCGG 
TTCGCGGCGA TGGCCGTCAA CAACTCGGGC TCGCTGCTGT TCGCGGCCAC GGACGATAGC 
CGGGTCGTTT GTGTCGACTT GAAAACTAAC TCAAGAGTTT TCGATATCGA GAACAGAAGG 
GGCAATGTGG TGTCAATGGC GGTGAGCGAA GTGCTGATGT GGGATGAATT CGAACCGAGC 
TCCGACGTAC TTCTAACCGG TACTGATAGT ACCGAAAATT CTGCCAAAGT TTGGTATTTA 
GACGCTTCAT ACGTGTCGCA GACTGCACAA AGAAAAGGAA AAGTTCGTCT AACAAGTAAA 
TTCGACGTGA GTTTCCTAAA CGCGACAACG CCAACATCTC CGAGCGCCTC GCCTCTGAAC 
AGCGGCGTGG AAACAGTTCA CAACCCTCTA AAATCACACC AGTCGTTCAC GAACAAGGAC 
CCAGTAAAGA AACCGATTAA AACTACCATG AGCTTGGACA GGAATACATT GAAACCGCTC 
AATCTCAAAG GAATATGCAA CAACAATGAA GGGCTCATAC ACCCATTACT GGCTGTCGTC 
GATGATATGA ACAATATACA GGTGATGCGT GGTAAGAAAG TCCTAACCGA GATCTTAGAG 
AAATCTGATG AGAAGATAAC AGCCGTCAAG GTATCGCCGT GCAATCACTA CATCATGTAC 
GGGCTCAAGT CGGGAAAAGT CAAGAGATAC ACATTGAGAA CCAAGGAGAT GACAACTATA 
ATGCACGTTA ACAGTCCGGT GCAATACCTG AACTACATCA ACAACAGGTT GATGCTGGTC 
GCGTGGAAGA ACCGATGTCT AATGTCGTAT AAACTGGGCC ACGAGGAGAC CTGGAAGACT 
GAAATGCTAC AGGCCGGTAA CACGCACCTC GGCTCGCAAG AATCGCAGCT AGGAAGCGAT 
TGGCAGAGTA TGAAAATAAG GAACGGCCAC TCCGACTTGT CGACGTCGAG CAGCGAGGCC 
AGCGTGAGCT CCCGCGAGAA CCGACTGTTC CACGGCGGGG AGATGAAGGA GCTGTACAAA 
CAGAGCGACC TCGTCGACTG CTTCTGGGTC GCGGACACCG GCCTCATCAC GGTCGAGTCG 
AACGCGGCCA TCAAACTGTG GAACAAAGAC TGCAAAGTCT CAACCATATT GAACGCGAGG 
CAAATGGATA CTTACATCAC GTGCGCCGCT TTCCAGAACA ACGTTCTCGT TATATGCGAT 
AGTGAAAACA AAGGATTTCA GTCGTACGAA CTAAAATTCG ACAACACAAT AGATTTGTGC 
CTCATCCAAG AGTACAAACT GAACAATGTG GTGACGTCAT GCGATATCAC ATCCGACGGC 
TACATACTAG CGATGGGACT AGACTGTGGT AATGTTGTGC TTTGGAACGT AAAAGGGAAG 
CGTCAAATCA GTTTACTCAA ACACCACAAC TCGAGAGTGC AGGCGTGCTC GTTCTCGCCG 
GTACCGGACC GACTGTACCG GAGCTCCGTG CACTCGCCCG GTGTGACGTC ACCGGCGGAC 
GCCGTCGCCG ACGACCAGCC TCCACTGGTC CTGGTCACGA TGGCTTCAGA GATAGTCTGG 
TGGAACATCA CGTACGTCAT ACGGATACGA GCGAGTAATA AGTCATTGTG GAGGAGCGGA 
CTCAATGTTA TATCGCCAAT TGGAACGCCA TTAGAGAATC GAGCTGAGAA TCACCAGACG 
ACAGAAAATT CGGGCAACGT TAATACCAAT TTCTTCTTCA GAGATATGTT CGCAGATAGA 
CATTGTTGGA AGAACATTTG GAAAGGAAAA ACATGTAAAG AAGGGTCGAA ACGCAAAGAA 
ATATTGGCGT GTATAAAACT ATCGGGTATA AACGCGACGA GGTTGTGCCA CGACGAGAAT 
TTCTCGTGCT TCGTGACCGT GGACAATCCG GGCCACGTCC ACATCATGAA CGTTATGAGG 
GATCTTTGTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apaf-1 ( XM_021353580.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021209255.1 Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X2, mRNA.

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CloneID	OBg22410
Clone ID Related Accession (Same CDS sequence)	XM_012697713.2 , XM_021353580.1
Accession Version	XM_021353580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	apaf-1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTCA AGAACAGGAA GCTGCTGCAG CACAATCAGC AGGCTATCAT CAAAGATCTA 
GATGTTCAGT ACATTCTAGA CGAACTTTAC ACGAAACAAG CTATATCTTC AGAGGTGTAC 
GAACATATCG CTAATCTGGT CATAGTTAAA AATCGAGTCG AAGGCGTCAG GTATTTGATA 
GATTGCCTCC TAAGGTATGG CACGAATCAG GCCTACGAAG CATTCGTTGA TAGCCTTGCC 
AAAGATTACA ACTGGCTCTG GGAGAAGTTT GCTACACAAT CCAACGGGCC AATGTTTAAC 
GACAGCTTCG AGGACAGCCT TAGCAAAGGC GACGTGCCAA GGCTTCCCGA ACACCATGTC 
AGGAGAAAGG CTGTGGAAAC TGAAGTCCTA TCCAAGATTA AAGAGTTGAC CCGTCACAAG 
ATACTGGTGC TTCACGGTAT GCTTGGCAGC GGAAAGACCT GCGTCGCCAT CAACGTACTC 
CGGGACAACC CCGAACTCAT AACGAACAAT TTCAATGATA TTGTCTTTTG GATGAACTTT 
GCGAATTGCA AGACCGATGA CGACATCATC GCTCAACAGA ATACGTTACT ACGTAAAATA 
TCATCGATGT ACATTCAAAA TTCTCACATG AACTCTTCGA TATCAATGTC GAGTATGGGC 
TCGAATATCG ACAGCCATTC GCTCTCCTAC TACGATCGCA CGTGGAACGA CTTCAGAGAC 
AGACTGAAGA ACCTATTCTC GGAGCCCCCG CTGAAGGAAG GTTTGCTCGT AATGGACGAA 
GTCAACGATC GGAAATGCGT CGAAGCGTTC GATATCGGTT GCAAGATACT GATCACGACC 
AGAGATACGG ACGTTGTGGC TAATTATCAC CCGCAAATCG TTACGGTGGA GAACAGTTTC 
ACAGAGAGGG AGTCCCTAGA ACTGTTTGCT TCTTGCTTAG ACGAACCTGT ATCGAAACTT 
CCATCGTATG CGAAAGAGCT CAACGAATTA TGCAAAGGAA GCCCCTTCCA CGTGGCCCTG 
ATCGGTGCCC AGCTCGCAGA AGACAGGGAG AGACTGAAAC ACGATCGAAG TCATTGGAAA 
TATTACTTGA AGAAATTAAA AAAGAATTTC TTTTCTGTGT GGAAAACGAA CAACGATAAC 
CCGATGAAAA CTATACAAGT GTGTATAAAC TCGTTGAACC CCAACACGCT TGCGATGTTC 
ATGAAACTGG CAGTGCTTCC GGACAGCGTC AAAGTCACCG CTCAAGTCTA CAGCAAACTG 
TGGAACGTAG ACGTCAACGA AGTCGAAAAC ATAACGAAGA AACTCAAGAG CAAGTCTCTG 
ATCATAAAGA ACTACGATAG AGATCAAAAA AAGTATTTAT ACGAAGTGCA CGATCTCATA 
ATGAACTATC TAAGGTCTTG TACGCACGAA GAGGAAATGA AGAAGCTACA CGACGAACTG 
TTGAACAGTT ACAGATATGA TAGCGAAACT GCACCAACGG AGATAGTCGA TGACGGATAC 
ATAGCCTTCT ACATAGGATT TCACATTTTG AAAACGAAAA ATTTGCACCA GAAATGGAGT 
TTGTTCAGTA AGCTGTATTT AGATTTGAGG TTTCTAGGGA ATAAAGTCAG ACTGACCGGA 
CCGGACGATG TCATGCTGGA TCTGCAGAAA TACGAAAATT ATATTGTGAG AGATGAAATC 
GACAGAAACT TAGTGTACAG TATAAAAGCG TTTCTAAGCA CGCACGGCAC AGACCTGCAC 
CACTACCCCT GCACGAACAT TGTGCAAAGT ATAATACAAC ACGAAACGAA AGGAATATTG 
TGTTGTAAGG CTTCGTTAAT AGCACGGGAT AACTGCGTAA ACAACGAATT GTATTTTGAG 
TTTATGCACG AACATGATGT AGAAGAAATT AAACATTCAA CTATCGACGT TAAGGAAGTT 
ATCAAATCTG TTTGCTTCTT GAGAAATTAT GTAATTGTTG GGACGTCCAA TGGCTTGATA 
AAGTTCTTCA ACATAGAAAC TAATAAGCAG AAGAAGGAAC TAAAGGGCAC CGGTGCCGCC 
ATTAAATGGA TAGGCGTCGC GTGCACTAAA GACACGACTA TTGTAGCCGC TCTGTCGACG 
GACGGATATC TCAAACTTTG GTTCGTCGAT GACTTCGATG ATGACGACAA CGCTAATAAT 
CTCGTCGAAG AAGAACCGAA CGATGCCTAC AACAACTATC CCAGTCCGTT AACATTTGAA 
CCCAAACTGG GACCGATACT AAACTGTCGT TGGACGACCA GCGAAGACTT GTTAATAACT 
CACACGTCAC AAATGATTAT ACTGTACCGT CCCAAAGGCG CCGAGTATGA CGTCATAAGT 
GAGTTCGACA GGGAACAGAA GCTGCTATGC TGCGTCCCCT GTAACAACGA CAAATACATT 
GCTGTAGCTA GTTTCAACGC CGTATACAGA GTGGACTTGA TCGACATTAG AACTAAGCAG 
AAAGTTGTTT ATTTCGAAGA AACAGGTGCC ATATTGGATA TGCTTGTTGT TCCTGGTACC 
AATAAAATAA TAACGCTGAA ACAAAACGAG ATAATGGCAC ACGAGTTCCG GGTAAAGCCT 
GCTTCGATAA ACAGAGGCGG GACGGTTATC GATAGCGGTG CGGTCAAAGC GGATGTCCGG 
TTCGCGGCGA TGGCCGTCAA CAACTCGGGC TCGCTGCTGT TCGCGGCCAC GGACGATAGC 
CGGGTCGTTT GTGTCGACTT GAAAACTAAC TCAAGAGTTT TCGATATCGA GAACAGAAGG 
GGCAATGTGG TGTCAATGGC GGTGAGCGAA GTGCTGATGT GGGATGAATT CGAACCGAGC 
TCCGACGTAC TTCTAACCGG TACTGATAGT ACCGAAAATT CTGCCAAAGT TTGGTATTTA 
GACGCTTCAT ACGTGTCGCA GACTGCACAA AGAAAAGGAA AAGTTCGTCT AACAAGTAAA 
TTCGACGTGA GTTTCCTAAA CGCGACAACG CCAACATCTC CGAGCGCCTC GCCTCTGAAC 
AGCGGCGTGG AAACAGTTCA CAACCCTCTA AAATCACACC AGTCGTTCAC GAACAAGGAC 
CCAGTAAAGA AACCGATTAA AACTACCATG AGCTTGGACA GGAATACATT GAAACCGCTC 
AATCTCAAAG GAATATGCAA CAACAATGAA GGGCTCATAC ACCCATTACT GGCTGTCGTC 
GATGATATGA ACAATATACA GGTGATGCGT GGTAAGAAAG TCCTAACCGA GATCTTAGAG 
AAATCTGATG AGAAGATAAC AGCCGTCAAG GTATCGCCGT GCAATCACTA CATCATGTAC 
GGGCTCAAGT CGGGAAAAGT CAAGAGATAC ACATTGAGAA CCAAGGAGAT GACAACTATA 
ATGCACGTTA ACAGTCCGGT GCAATACCTG AACTACATCA ACAACAGGTT GATGCTGGTC 
GCGTGGAAGA ACCGATGTCT AATGTCGTAT AAACTGGGCC ACGAGGAGAC CTGGAAGACT 
GAAATGCTAC AGGCCGGTAA CACGCACCTC GGCTCGCAAG AATCGCAGCT AGGAAGCGAT 
TGGCAGAGTA TGAAAATAAG GAACGGCCAC TCCGACTTGT CGACGTCGAG CAGCGAGGCC 
AGCGTGAGCT CCCGCGAGAA CCGACTGTTC CACGGCGGGG AGATGAAGGA GCTGTACAAA 
CAGAGCGACC TCGTCGACTG CTTCTGGGTC GCGGACACCG GCCTCATCAC GGTCGAGTCG 
AACGCGGCCA TCAAACTGTG GAACAAAGAC TGCAAAGTCT CAACCATATT GAACGCGAGG 
CAAATGGATA CTTACATCAC GTGCGCCGCT TTCCAGAACA ACGTTCTCGT TATATGCGAT 
AGTGAAAACA AAGGATTTCA GTCGTACGAA CTAAAATTCG ACAACACAAT AGATTTGTGC 
CTCATCCAAG AGTACAAACT GAACAATGTG GTGACGTCAT GCGATATCAC ATCCGACGGC 
TACATACTAG CGATGGGACT AGACTGTGGT AATGTTGTGC TTTGGAACGT AAAAGGGAAG 
CGTCAAATCA GTTTACTCAA ACACCACAAC TCGAGAGTGC AGGCGTGCTC GTTCTCGCCG 
GTACCGGACC GACTGTACCG GAGCTCCGTG CACTCGCCCG GTGTGACGTC ACCGGCGGAC 
GCCGTCGCCG ACGACCAGCC TCCACTGGTC CTGGTCACGA TGGCTTCAGA GATAGTCTGG 
TGGAACATCA CGTACGTCAT ACGGATACGA GCGAGTAATA AGTCATTGTG GAGGAGCGGA 
CTCAATGTTA TATCGCCAAT TGGAACGCCA TTAGAGAATC GAGCTGAGAA TCACCAGACG 
ACAGAAAATT CGGGCAACGT TAATACCAAT TTCTTCTTCA GAGATATGTT CGCAGATAGA 
CATTGTTGGA AGAACATTTG GAAAGGAAAA ACATGTAAAG AAGGGTCGAA ACGCAAAGAA 
ATATTGGCGT GTATAAAACT ATCGGGTATA AACGCGACGA GGTTGTGCCA CGACGAGAAT 
TTCTCGTGCT TCGTGACCGT GGACAATCCG GGCCACGTCC ACATCATGAA CGTTATGAGG 
GATCTTTGTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021209255.1
CDS	1043..5617
Translation	MDLKNRKLLQHNQQAIIKDLDVQYILDELYTKQAISSEVYEHIANLVIVKNRVEGVRYLIDCLLRYGTNQAYEAFVDSLAKDYNWLWEKFATQSNGPMFNDSFEDSLSKGDVPRLPEHHVRRKAVETEVLSKIKELTRHKILVLHGMLGSGKTCVAINVLRDNPELITNNFNDIVFWMNFANCKTDDDIIAQQNTLLRKISSMYIQNSHMNSSISMSSMGSNIDSHSLSYYDRTWNDFRDRLKNLFSEPPLKEGLLVMDEVNDRKCVEAFDIGCKILITTRDTDVVANYHPQIVTVENSFTERESLELFASCLDEPVSKLPSYAKELNELCKGSPFHVALIGAQLAEDRERLKHDRSHWKYYLKKLKKNFFSVWKTNNDNPMKTIQVCINSLNPNTLAMFMKLAVLPDSVKVTAQVYSKLWNVDVNEVENITKKLKSKSLIIKNYDRDQKKYLYEVHDLIMNYLRSCTHEEEMKKLHDELLNSYRYDSETAPTEIVDDGYIAFYIGFHILKTKNLHQKWSLFSKLYLDLRFLGNKVRLTGPDDVMLDLQKYENYIVRDEIDRNLVYSIKAFLSTHGTDLHHYPCTNIVQSIIQHETKGILCCKASLIARDNCVNNELYFEFMHEHDVEEIKHSTIDVKEVIKSVCFLRNYVIVGTSNGLIKFFNIETNKQKKELKGTGAAIKWIGVACTKDTTIVAALSTDGYLKLWFVDDFDDDDNANNLVEEEPNDAYNNYPSPLTFEPKLGPILNCRWTTSEDLLITHTSQMIILYRPKGAEYDVISEFDREQKLLCCVPCNNDKYIAVASFNAVYRVDLIDIRTKQKVVYFEETGAILDMLVVPGTNKIITLKQNEIMAHEFRVKPASINRGGTVIDSGAVKADVRFAAMAVNNSGSLLFAATDDSRVVCVDLKTNSRVFDIENRRGNVVSMAVSEVLMWDEFEPSSDVLLTGTDSTENSAKVWYLDASYVSQTAQRKGKVRLTSKFDVSFLNATTPTSPSASPLNSGVETVHNPLKSHQSFTNKDPVKKPIKTTMSLDRNTLKPLNLKGICNNNEGLIHPLLAVVDDMNNIQVMRGKKVLTEILEKSDEKITAVKVSPCNHYIMYGLKSGKVKRYTLRTKEMTTIMHVNSPVQYLNYINNRLMLVAWKNRCLMSYKLGHEETWKTEMLQAGNTHLGSQESQLGSDWQSMKIRNGHSDLSTSSSEASVSSRENRLFHGGEMKELYKQSDLVDCFWVADTGLITVESNAAIKLWNKDCKVSTILNARQMDTYITCAAFQNNVLVICDSENKGFQSYELKFDNTIDLCLIQEYKLNNVVTSCDITSDGYILAMGLDCGNVVLWNVKGKRQISLLKHHNSRVQACSFSPVPDRLYRSSVHSPGVTSPADAVADDQPPLVLVTMASEIVWWNITYVIRIRASNKSLWRSGLNVISPIGTPLENRAENHQTTENSGNVNTNFFFRDMFADRHCWKNIWKGKTCKEGSKRKEILACIKLSGINATRLCHDENFSCFVTVDNPGHVHIMNVMRDLCT

Target ORF information:

RefSeq Version	XM_021353580.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori Apaf-1 protein (Apaf-1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021353580.1

ORF Insert Sequence:

ATGGATCTCA AGAACAGGAA GCTGCTGCAG CACAATCAGC AGGCTATCAT CAAAGATCTA 
GATGTTCAGT ACATTCTAGA CGAACTTTAC ACGAAACAAG CTATATCTTC AGAGGTGTAC 
GAACATATCG CTAATCTGGT CATAGTTAAA AATCGAGTCG AAGGCGTCAG GTATTTGATA 
GATTGCCTCC TAAGGTATGG CACGAATCAG GCCTACGAAG CATTCGTTGA TAGCCTTGCC 
AAAGATTACA ACTGGCTCTG GGAGAAGTTT GCTACACAAT CCAACGGGCC AATGTTTAAC 
GACAGCTTCG AGGACAGCCT TAGCAAAGGC GACGTGCCAA GGCTTCCCGA ACACCATGTC 
AGGAGAAAGG CTGTGGAAAC TGAAGTCCTA TCCAAGATTA AAGAGTTGAC CCGTCACAAG 
ATACTGGTGC TTCACGGTAT GCTTGGCAGC GGAAAGACCT GCGTCGCCAT CAACGTACTC 
CGGGACAACC CCGAACTCAT AACGAACAAT TTCAATGATA TTGTCTTTTG GATGAACTTT 
GCGAATTGCA AGACCGATGA CGACATCATC GCTCAACAGA ATACGTTACT ACGTAAAATA 
TCATCGATGT ACATTCAAAA TTCTCACATG AACTCTTCGA TATCAATGTC GAGTATGGGC 
TCGAATATCG ACAGCCATTC GCTCTCCTAC TACGATCGCA CGTGGAACGA CTTCAGAGAC 
AGACTGAAGA ACCTATTCTC GGAGCCCCCG CTGAAGGAAG GTTTGCTCGT AATGGACGAA 
GTCAACGATC GGAAATGCGT CGAAGCGTTC GATATCGGTT GCAAGATACT GATCACGACC 
AGAGATACGG ACGTTGTGGC TAATTATCAC CCGCAAATCG TTACGGTGGA GAACAGTTTC 
ACAGAGAGGG AGTCCCTAGA ACTGTTTGCT TCTTGCTTAG ACGAACCTGT ATCGAAACTT 
CCATCGTATG CGAAAGAGCT CAACGAATTA TGCAAAGGAA GCCCCTTCCA CGTGGCCCTG 
ATCGGTGCCC AGCTCGCAGA AGACAGGGAG AGACTGAAAC ACGATCGAAG TCATTGGAAA 
TATTACTTGA AGAAATTAAA AAAGAATTTC TTTTCTGTGT GGAAAACGAA CAACGATAAC 
CCGATGAAAA CTATACAAGT GTGTATAAAC TCGTTGAACC CCAACACGCT TGCGATGTTC 
ATGAAACTGG CAGTGCTTCC GGACAGCGTC AAAGTCACCG CTCAAGTCTA CAGCAAACTG 
TGGAACGTAG ACGTCAACGA AGTCGAAAAC ATAACGAAGA AACTCAAGAG CAAGTCTCTG 
ATCATAAAGA ACTACGATAG AGATCAAAAA AAGTATTTAT ACGAAGTGCA CGATCTCATA 
ATGAACTATC TAAGGTCTTG TACGCACGAA GAGGAAATGA AGAAGCTACA CGACGAACTG 
TTGAACAGTT ACAGATATGA TAGCGAAACT GCACCAACGG AGATAGTCGA TGACGGATAC 
ATAGCCTTCT ACATAGGATT TCACATTTTG AAAACGAAAA ATTTGCACCA GAAATGGAGT 
TTGTTCAGTA AGCTGTATTT AGATTTGAGG TTTCTAGGGA ATAAAGTCAG ACTGACCGGA 
CCGGACGATG TCATGCTGGA TCTGCAGAAA TACGAAAATT ATATTGTGAG AGATGAAATC 
GACAGAAACT TAGTGTACAG TATAAAAGCG TTTCTAAGCA CGCACGGCAC AGACCTGCAC 
CACTACCCCT GCACGAACAT TGTGCAAAGT ATAATACAAC ACGAAACGAA AGGAATATTG 
TGTTGTAAGG CTTCGTTAAT AGCACGGGAT AACTGCGTAA ACAACGAATT GTATTTTGAG 
TTTATGCACG AACATGATGT AGAAGAAATT AAACATTCAA CTATCGACGT TAAGGAAGTT 
ATCAAATCTG TTTGCTTCTT GAGAAATTAT GTAATTGTTG GGACGTCCAA TGGCTTGATA 
AAGTTCTTCA ACATAGAAAC TAATAAGCAG AAGAAGGAAC TAAAGGGCAC CGGTGCCGCC 
ATTAAATGGA TAGGCGTCGC GTGCACTAAA GACACGACTA TTGTAGCCGC TCTGTCGACG 
GACGGATATC TCAAACTTTG GTTCGTCGAT GACTTCGATG ATGACGACAA CGCTAATAAT 
CTCGTCGAAG AAGAACCGAA CGATGCCTAC AACAACTATC CCAGTCCGTT AACATTTGAA 
CCCAAACTGG GACCGATACT AAACTGTCGT TGGACGACCA GCGAAGACTT GTTAATAACT 
CACACGTCAC AAATGATTAT ACTGTACCGT CCCAAAGGCG CCGAGTATGA CGTCATAAGT 
GAGTTCGACA GGGAACAGAA GCTGCTATGC TGCGTCCCCT GTAACAACGA CAAATACATT 
GCTGTAGCTA GTTTCAACGC CGTATACAGA GTGGACTTGA TCGACATTAG AACTAAGCAG 
AAAGTTGTTT ATTTCGAAGA AACAGGTGCC ATATTGGATA TGCTTGTTGT TCCTGGTACC 
AATAAAATAA TAACGCTGAA ACAAAACGAG ATAATGGCAC ACGAGTTCCG GGTAAAGCCT 
GCTTCGATAA ACAGAGGCGG GACGGTTATC GATAGCGGTG CGGTCAAAGC GGATGTCCGG 
TTCGCGGCGA TGGCCGTCAA CAACTCGGGC TCGCTGCTGT TCGCGGCCAC GGACGATAGC 
CGGGTCGTTT GTGTCGACTT GAAAACTAAC TCAAGAGTTT TCGATATCGA GAACAGAAGG 
GGCAATGTGG TGTCAATGGC GGTGAGCGAA GTGCTGATGT GGGATGAATT CGAACCGAGC 
TCCGACGTAC TTCTAACCGG TACTGATAGT ACCGAAAATT CTGCCAAAGT TTGGTATTTA 
GACGCTTCAT ACGTGTCGCA GACTGCACAA AGAAAAGGAA AAGTTCGTCT AACAAGTAAA 
TTCGACGTGA GTTTCCTAAA CGCGACAACG CCAACATCTC CGAGCGCCTC GCCTCTGAAC 
AGCGGCGTGG AAACAGTTCA CAACCCTCTA AAATCACACC AGTCGTTCAC GAACAAGGAC 
CCAGTAAAGA AACCGATTAA AACTACCATG AGCTTGGACA GGAATACATT GAAACCGCTC 
AATCTCAAAG GAATATGCAA CAACAATGAA GGGCTCATAC ACCCATTACT GGCTGTCGTC 
GATGATATGA ACAATATACA GGTGATGCGT GGTAAGAAAG TCCTAACCGA GATCTTAGAG 
AAATCTGATG AGAAGATAAC AGCCGTCAAG GTATCGCCGT GCAATCACTA CATCATGTAC 
GGGCTCAAGT CGGGAAAAGT CAAGAGATAC ACATTGAGAA CCAAGGAGAT GACAACTATA 
ATGCACGTTA ACAGTCCGGT GCAATACCTG AACTACATCA ACAACAGGTT GATGCTGGTC 
GCGTGGAAGA ACCGATGTCT AATGTCGTAT AAACTGGGCC ACGAGGAGAC CTGGAAGACT 
GAAATGCTAC AGGCCGGTAA CACGCACCTC GGCTCGCAAG AATCGCAGCT AGGAAGCGAT 
TGGCAGAGTA TGAAAATAAG GAACGGCCAC TCCGACTTGT CGACGTCGAG CAGCGAGGCC 
AGCGTGAGCT CCCGCGAGAA CCGACTGTTC CACGGCGGGG AGATGAAGGA GCTGTACAAA 
CAGAGCGACC TCGTCGACTG CTTCTGGGTC GCGGACACCG GCCTCATCAC GGTCGAGTCG 
AACGCGGCCA TCAAACTGTG GAACAAAGAC TGCAAAGTCT CAACCATATT GAACGCGAGG 
CAAATGGATA CTTACATCAC GTGCGCCGCT TTCCAGAACA ACGTTCTCGT TATATGCGAT 
AGTGAAAACA AAGGATTTCA GTCGTACGAA CTAAAATTCG ACAACACAAT AGATTTGTGC 
CTCATCCAAG AGTACAAACT GAACAATGTG GTGACGTCAT GCGATATCAC ATCCGACGGC 
TACATACTAG CGATGGGACT AGACTGTGGT AATGTTGTGC TTTGGAACGT AAAAGGGAAG 
CGTCAAATCA GTTTACTCAA ACACCACAAC TCGAGAGTGC AGGCGTGCTC GTTCTCGCCG 
GTACCGGACC GACTGTACCG GAGCTCCGTG CACTCGCCCG GTGTGACGTC ACCGGCGGAC 
GCCGTCGCCG ACGACCAGCC TCCACTGGTC CTGGTCACGA TGGCTTCAGA GATAGTCTGG 
TGGAACATCA CGTACGTCAT ACGGATACGA GCGAGTAATA AGTCATTGTG GAGGAGCGGA 
CTCAATGTTA TATCGCCAAT TGGAACGCCA TTAGAGAATC GAGCTGAGAA TCACCAGACG 
ACAGAAAATT CGGGCAACGT TAATACCAAT TTCTTCTTCA GAGATATGTT CGCAGATAGA 
CATTGTTGGA AGAACATTTG GAAAGGAAAA ACATGTAAAG AAGGGTCGAA ACGCAAAGAA 
ATATTGGCGT GTATAAAACT ATCGGGTATA AACGCGACGA GGTTGTGCCA CGACGAGAAT 
TTCTCGTGCT TCGTGACCGT GGACAATCCG GGCCACGTCC ACATCATGAA CGTTATGAGG 
GATCTTTGTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.
BMC genomics11611(2010 Oct)
Zhang JY,Pan MH,Sun ZY,Huang SJ,Yu ZS,Liu D,Zhao DH,Lu C

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Apaf-1 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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