nos1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	nos1
Entrez Gene ID	103046065
Full Name	nitric oxide synthase 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015604434.1	XP_015459920.1	nitric oxide synthase, brain
XM_022667296.1	XP_022523017.1	LOW QUALITY PROTEIN: nitric oxide synthase, brain

Pathways from BioSystems
Gene Ontology

The following nos1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs102442	XM_022667296.1 Latest version!	Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAs14487	XM_015604434.1 Latest version!	Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nos1 ( XM_022667296.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022523017.1 Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.

Price & Availability↑

CloneID	OAs102442
Clone ID Related Accession (Same CDS sequence)	XM_022667296.1
Accession Version	XM_022667296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	LOW QUALITY PROTEIN: nitric oxide synthase, brain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019172901.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACGGATT CCGAGCCCAG CGTATGCCAG CTCCAGCCCA ACATCATTTC TGTGCGTCTC 
TTCAAGCGCA AGATTGGTGG ACTGGGCTTC CTGGTGAAGC AGAGGGTCTG CAAACCCCCC 
GTCATCATCT CGGACCTGAT CCGGGGCGGA GCGGCGGAAG AGTGTGGCCT AGTGCAGGTG 
GGTGACATCG TGCTGGCCGT CAACAACAAG CCGCTGGTGG ACCTGAGCTA CGAGCGAGCG 
CTGGAGACCC TGAAGAACGT CTCACCCGAA AGCCACGCTG TGCTGATCCT GCGGGGTCCT 
GAGGGCTTCA CCACACACCT GGAGACCACC CTGAGCGGAG ACGGGAGACC CAGGACCATC 
CGCATCACCA AACCCGTGTC CAGGCCCTGT GAACGGTGCT CGCCCCTGCA GCCGGGAACA 
CAGGCCAAAG AGCTGAGGGC CATTGAGAAC CTGTCGTCAG CTTCCACCCC CAAAAAAGAG 
GCCAAAGATG CCAAGAACTC CCTGATTGGA CAGGACGCCC TGCTGAGGGA GGGGGGTGGC 
GGCCTGCTGC TGAACGGAGT GGACGATGGG AATGACCTGC TAAAGGAGAT CGAGCCGGTT 
CTGAAGCTGA TCAAGAACAG CAACAGCAAG AAGGAGGTGA ATGGAGACGG ACAGAGAGGG 
GTGGAGAAGA AGGACGTGGA AATACAAGTG GAAAGAGACC TTGGTGTGGG AATAGGCAGC 
AATATCCAGC TGGCCGCCGA TAACGACAGA GTGTTTGATG ACCTCTGGAG GAAGGACAAC 
ATGCCCACGG TGCTCAACAA CCCGTACTCC GAGGGCGACA AGCCACCGTC ACAAGGCAAT 
NCATCACCAA CCAAGACTGT ACAAAATGGA AGTCCAACCA AATGCCCCAG ATTCATGAGG 
GTAAAGAACT GGGAGAGTGG GGCGGTGTTA AACGATACCC TACACCACAA CTCCAGCAAG 
GTGCCAATTT GCAACGAGAG GGTGTGTATA GGCTCTATTA TGACGCCGAG CCAGAACCGA 
AAACCAGATG AAGTCCGAAC CAAAGAAGAG CTGCTGCCGC TGGCCACAGA CTTCATCAAC 
CAGTACTACA CTTCAATCAA AAGGTGTGGA TCTAAGGCGC ATGTGGATCG ACTGGAAGAG 
GTGAAGAAAG AGATTGAAAC TTCTGGAACG TACCAGCTCA AAGACACAGA GCTGATATAT 
GGAGCCAAAC ATGCATGGAG GAATGCTGCC CGCTGTGTGG GAAGAATCCA GTGGTCCAAA 
CTACAGGTGT TTGATGCCCG AGACTGCACC ACAGCACACG GAATGTTTAA CTACATCTGC 
AATCACATCA AATACGCCAC TAACAAGGGC AACCTCAGGT CAGCTATCAC CATTTTCCCC 
CCGAGGACAG ACGGGAAGCA CGATTTCCGA GTGTGGAACA GCCAGCTGAT CCGCTACGCC 
GGCTACAAGC AGCCCGACGG CTCAGTGCTG GGAGACCCGG CGTCCGTGGA GCTCACCGAG 
ATCTGTATAC AGCAGGGATG GAAGGCTCCT AAAGGGCGTT TCGATGTCCT GCCTCTTCTG 
CTCCAGGCCA ACGGCAATGA CCCAGAGCTG TTCGAGATTC CAGATGACCT CGTGCTCGAG 
GTCAACATCA CACACCCCAA GTTCGAGTGG TTTAAGGAGC TGAATCTGAA GTGGTACGGT 
TTGCCGGCGG TCTCTAACAT GCTGCTGGAG ATCGGGGGGC TGGAGTTCAC CGCCTGCCCG 
TTCAGCGGTT GGTACATGGG CACGGAGATT GGCGTCAGAG ACTTCTGCGA CAGCTCTCGC 
TACAACCTGC TGGAGGAAGT GGCCACCATG ATGGGGCTGG ACACCAGGAA AACGTCCTCG 
CTGTGGAAGG ACCAGGCTCT GGTAGAGATC AACATCGCTG TGCTTTACAG TTACCAGGTC 
TCTAAGGTGA CCATCGTGGA TCATCACTCA GCTACAGAGT CGTTTATTAA GCACATGGAG 
AACGAGTACA GGGTACGGGG CGGATGCCCG GGTGACTGGG TGTGGATCGT TCCTCCAATG 
TCTGGAAGCA TCACGCCTGT CTTCCACCAG GAGATGCTCA ACTACAGACT CACTCCGTCC 
TTTGAGTATC AGCCTGATCC GTGGAACACT CATGTGTGGA AAGGTGTGAA CGGAACGCCC 
ACCAAGAAAC GAGCCATCGG CTTCAAGAAA CTTGCGAAGG CCGTGAAATT CTCAGCCAAG 
CTGATGGGTC AAGCCATGGC GAAGCGAGTG AAGGCCACCA TCCTGTTCGC CACAGAGACG 
GGCAAATCTC AGGACTACGC AAAGACCCTC TGTGAGATCT TCAAGCACGC TTTTGATGCC 
AAGGTCATGT CGATGGAGGA GTATGACGTG GTGGATCTGG AGCACGAGAC GCTGGTGTTG 
GCGGTCACCA GCACGTTCGG AAACGGAGAC CCACCGGAGA ACGGCGAGAA ATTCGGTGCA 
GCGCTGATGG AGATGAGGCA CCCCACCACC AGCGCAGAGG ACAGAAAGAG TTACAAGGTT 
CGCTTTAACA GCGTCTCCTC GTACTCCGAC ACCCGGAAGT CCTCCAGCGA TGAACCCGAG 
TCTAAAGCCA ACTTCGAGAG CACTGGACCT CTCGCTAACG TCAGGTTCTC AGTGTTTGGC 
CTTGGCTCGC GGGCCTACCC ACACTTCTGC GCCTTTGCCC ACGCAGTGGA CACGCTGTTC 
GAGGAGCTCG GGGGGGAGCG CATCCTCCGC ATGGGCGAGG GAGACGAACT CTGCGGCCAG 
GAGGAGTCCT TCAGAACCTG GGCCAAGAAA GTTTTTAAGG CCGCCTGCGA CGTGTTCTGT 
GTAGGAGACG ACGTGAACAT CGAGAAGGCG AACGATTCGC TGATTAGTAA CGACCGCAGC 
TGGAAAAAGA GCAAGTTCCG CGTGACGTAC ACGGCCGAGG CTCCGGCGCT AACGCAAGCT 
CTCTACAACA TCCACAAGAA GAAGGTGTAT GGCGCCAAAG TCCTGATGAG ACAGAACCTT 
CAAAGCGTCA AATCAAACCG ATCCACCATA TACATCCAGC TCCACGCCAA CAACCACGAG 
AGTCTGAAAT ACCTGCCTGG AGATCACTTG GGCATCTACC CTGGCAACTA CGAGGACCTG 
GTGACGGCTC TCATAGACAA GCTGGAGGAT GCGCCGCCTG CCAATCAGAT CGTGAGGGTG 
GAGTTTCTGG AGGAGAGGAA CACAGCGCTG GGTGTGATCA GTAACTGGAC GGATGAGAGT 
CGTATTCCTC CCTGCACCAT CCATCAGGCC TTTAAATACT TTCTGGACAT CACGACTCCA 
CCCAGTCCAC TCCTGCTCCA ACAGTTCGCT CCTCTCGCCA CCAACGAGAA ACAGAGGAAG 
AGACTGGAGG TGCTCAGCAA GGGTCTGCAG GAGTATGAGG AGTGGAAGTG GTATAATAAC 
CCCACCATGG TGGAGGTGTT GGAGGAGTTC CCGTCGCTGC AGGTTCCCTG CACCCTGCTC 
CTCACCCAGC TCCCCCTGCT CCAGCCCCGC TACTACTCCA TCAGCTCCTC CCCAGACCTC 
CATCCAGGAG AGATCCACCT CACCGTGGCT GTGGTCTCCT ACCGTACCAG AGATGGTGCA 
GGTCCCGTAC ACCACGGGGT GTGTTCCTCC TGGCTCAACA GTGTGGAAGC GGGAGACATG 
GTGCCATGCT TCGTCAGACG AGCTCCATCA TTCCGGATGC CGAAGGACAG TAGAGTTCCC 
TGTATTCTGG TCGGGCCGGG AACAGGAATC GCTCCATTCA GAAGCTTCTG GCAACAGAGA 
CTCTACGACC TGGAAAATAA AGGGATAAAG GCCTGTCCGA TGATTTTAGT GTTCGGGTGT 
CGACAGTCTC AAATGGACCA CATCTACAAA GAAGAGACCA TCCAGGCCAA GAACAAGGAG 
GTGTTTAAGG AGCTCTACAC CGCTTACTCT CGAGAACCTG GAAGACCAAA GAAATATGTT 
CAGGATGTTC TGCGGGAGCA GTTATCTGAG ACGGTGTATA GGTGTTTGAG GGAGGAGGGG 
GGTCATATCT ACGTCTGTGG GGACGTCACC ATGGCCGGAG ACGTGCTGAA GACCGTCCAG 
CAGATCGTCA AACTGCAAGG CAACATGTCC CTGGAGGACG CGGGATTCTA CATCAGCAAA 
CTGAGGGATG AGAATCGCTA CCATGAAGAC ATTTTCGGCG TGACTCTACG TACGTACGAG 
GTCACGAACC GGCTGCGGTC AGAATCCATC GCATACATCG AGGAAAGCAA GAAAGACTCA 
GACGAGGTGT TTTGTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022523017.1
CDS	295..4575
Translation	MTDSEPSVCQLQPNIISVRLFKRKIGGLGFLVKQRVCKPPVIISDLIRGGAAEECGLVQVGDIVLAVNNKPLVDLSYERALETLKNVSPESHAVLILRGPEGFTTHLETTLSGDGRPRTIRITKPVSRPCERCSPLQPGTQAKELRAIENLSSASTPKKEAKDAKNSLIGQDALLREGGGGLLLNGVDDGNDLLKEIEPVLKLIKNSNSKKEVNGDGQRGVEKKDVEIQVERDLGVGIGSNIQLAADNDRVFDDLWRKDNMPTVLNNPYSEGDKPPSQGNnullSPTKTVQNGSPTKCPRFMRVKNWESGAVLNDTLHHNSSKVPICNERVCIGSIMTPSQNRKPDEVRTKEELLPLATDFINQYYTSIKRCGSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPPRTDGKHDFRVWNSQLIRYAGYKQPDGSVLGDPASVELTEICIQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVNITHPKFEWFKELNLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDFCDSSRYNLLEEVATMMGLDTRKTSSLWKDQALVEINIAVLYSYQVSKVTIVDHHSATESFIKHMENEYRVRGGCPGDWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILFATETGKSQDYAKTLCEIFKHAFDAKVMSMEEYDVVDLEHETLVLAVTSTFGNGDPPENGEKFGAALMEMRHPTTSAEDRKSYKVRFNSVSSYSDTRKSSSDEPESKANFESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANDSLISNDRSWKKSKFRVTYTAEAPALTQALYNIHKKKVYGAKVLMRQNLQSVKSNRSTIYIQLHANNHESLKYLPGDHLGIYPGNYEDLVTALIDKLEDAPPANQIVRVEFLEERNTALGVISNWTDESRIPPCTIHQAFKYFLDITTPPSPLLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQVPCTLLLTQLPLLQPRYYSISSSPDLHPGEIHLTVAVVSYRTRDGAGPVHHGVCSSWLNSVEAGDMVPCFVRRAPSFRMPKDSRVPCILVGPGTGIAPFRSFWQQRLYDLENKGIKACPMILVFGCRQSQMDHIYKEETIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYRCLREEGGHIYVCGDVTMAGDVLKTVQQIVKLQGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTYEVTNRLRSESIAYIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_022667296.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022667296.1

ORF Insert Sequence:

ATGACGGATT CCGAGCCCAG CGTATGCCAG CTCCAGCCCA ACATCATTTC TGTGCGTCTC 
TTCAAGCGCA AGATTGGTGG ACTGGGCTTC CTGGTGAAGC AGAGGGTCTG CAAACCCCCC 
GTCATCATCT CGGACCTGAT CCGGGGCGGA GCGGCGGAAG AGTGTGGCCT AGTGCAGGTG 
GGTGACATCG TGCTGGCCGT CAACAACAAG CCGCTGGTGG ACCTGAGCTA CGAGCGAGCG 
CTGGAGACCC TGAAGAACGT CTCACCCGAA AGCCACGCTG TGCTGATCCT GCGGGGTCCT 
GAGGGCTTCA CCACACACCT GGAGACCACC CTGAGCGGAG ACGGGAGACC CAGGACCATC 
CGCATCACCA AACCCGTGTC CAGGCCCTGT GAACGGTGCT CGCCCCTGCA GCCGGGAACA 
CAGGCCAAAG AGCTGAGGGC CATTGAGAAC CTGTCGTCAG CTTCCACCCC CAAAAAAGAG 
GCCAAAGATG CCAAGAACTC CCTGATTGGA CAGGACGCCC TGCTGAGGGA GGGGGGTGGC 
GGCCTGCTGC TGAACGGAGT GGACGATGGG AATGACCTGC TAAAGGAGAT CGAGCCGGTT 
CTGAAGCTGA TCAAGAACAG CAACAGCAAG AAGGAGGTGA ATGGAGACGG ACAGAGAGGG 
GTGGAGAAGA AGGACGTGGA AATACAAGTG GAAAGAGACC TTGGTGTGGG AATAGGCAGC 
AATATCCAGC TGGCCGCCGA TAACGACAGA GTGTTTGATG ACCTCTGGAG GAAGGACAAC 
ATGCCCACGG TGCTCAACAA CCCGTACTCC GAGGGCGACA AGCCACCGTC ACAAGGCAAT 
NCATCACCAA CCAAGACTGT ACAAAATGGA AGTCCAACCA AATGCCCCAG ATTCATGAGG 
GTAAAGAACT GGGAGAGTGG GGCGGTGTTA AACGATACCC TACACCACAA CTCCAGCAAG 
GTGCCAATTT GCAACGAGAG GGTGTGTATA GGCTCTATTA TGACGCCGAG CCAGAACCGA 
AAACCAGATG AAGTCCGAAC CAAAGAAGAG CTGCTGCCGC TGGCCACAGA CTTCATCAAC 
CAGTACTACA CTTCAATCAA AAGGTGTGGA TCTAAGGCGC ATGTGGATCG ACTGGAAGAG 
GTGAAGAAAG AGATTGAAAC TTCTGGAACG TACCAGCTCA AAGACACAGA GCTGATATAT 
GGAGCCAAAC ATGCATGGAG GAATGCTGCC CGCTGTGTGG GAAGAATCCA GTGGTCCAAA 
CTACAGGTGT TTGATGCCCG AGACTGCACC ACAGCACACG GAATGTTTAA CTACATCTGC 
AATCACATCA AATACGCCAC TAACAAGGGC AACCTCAGGT CAGCTATCAC CATTTTCCCC 
CCGAGGACAG ACGGGAAGCA CGATTTCCGA GTGTGGAACA GCCAGCTGAT CCGCTACGCC 
GGCTACAAGC AGCCCGACGG CTCAGTGCTG GGAGACCCGG CGTCCGTGGA GCTCACCGAG 
ATCTGTATAC AGCAGGGATG GAAGGCTCCT AAAGGGCGTT TCGATGTCCT GCCTCTTCTG 
CTCCAGGCCA ACGGCAATGA CCCAGAGCTG TTCGAGATTC CAGATGACCT CGTGCTCGAG 
GTCAACATCA CACACCCCAA GTTCGAGTGG TTTAAGGAGC TGAATCTGAA GTGGTACGGT 
TTGCCGGCGG TCTCTAACAT GCTGCTGGAG ATCGGGGGGC TGGAGTTCAC CGCCTGCCCG 
TTCAGCGGTT GGTACATGGG CACGGAGATT GGCGTCAGAG ACTTCTGCGA CAGCTCTCGC 
TACAACCTGC TGGAGGAAGT GGCCACCATG ATGGGGCTGG ACACCAGGAA AACGTCCTCG 
CTGTGGAAGG ACCAGGCTCT GGTAGAGATC AACATCGCTG TGCTTTACAG TTACCAGGTC 
TCTAAGGTGA CCATCGTGGA TCATCACTCA GCTACAGAGT CGTTTATTAA GCACATGGAG 
AACGAGTACA GGGTACGGGG CGGATGCCCG GGTGACTGGG TGTGGATCGT TCCTCCAATG 
TCTGGAAGCA TCACGCCTGT CTTCCACCAG GAGATGCTCA ACTACAGACT CACTCCGTCC 
TTTGAGTATC AGCCTGATCC GTGGAACACT CATGTGTGGA AAGGTGTGAA CGGAACGCCC 
ACCAAGAAAC GAGCCATCGG CTTCAAGAAA CTTGCGAAGG CCGTGAAATT CTCAGCCAAG 
CTGATGGGTC AAGCCATGGC GAAGCGAGTG AAGGCCACCA TCCTGTTCGC CACAGAGACG 
GGCAAATCTC AGGACTACGC AAAGACCCTC TGTGAGATCT TCAAGCACGC TTTTGATGCC 
AAGGTCATGT CGATGGAGGA GTATGACGTG GTGGATCTGG AGCACGAGAC GCTGGTGTTG 
GCGGTCACCA GCACGTTCGG AAACGGAGAC CCACCGGAGA ACGGCGAGAA ATTCGGTGCA 
GCGCTGATGG AGATGAGGCA CCCCACCACC AGCGCAGAGG ACAGAAAGAG TTACAAGGTT 
CGCTTTAACA GCGTCTCCTC GTACTCCGAC ACCCGGAAGT CCTCCAGCGA TGAACCCGAG 
TCTAAAGCCA ACTTCGAGAG CACTGGACCT CTCGCTAACG TCAGGTTCTC AGTGTTTGGC 
CTTGGCTCGC GGGCCTACCC ACACTTCTGC GCCTTTGCCC ACGCAGTGGA CACGCTGTTC 
GAGGAGCTCG GGGGGGAGCG CATCCTCCGC ATGGGCGAGG GAGACGAACT CTGCGGCCAG 
GAGGAGTCCT TCAGAACCTG GGCCAAGAAA GTTTTTAAGG CCGCCTGCGA CGTGTTCTGT 
GTAGGAGACG ACGTGAACAT CGAGAAGGCG AACGATTCGC TGATTAGTAA CGACCGCAGC 
TGGAAAAAGA GCAAGTTCCG CGTGACGTAC ACGGCCGAGG CTCCGGCGCT AACGCAAGCT 
CTCTACAACA TCCACAAGAA GAAGGTGTAT GGCGCCAAAG TCCTGATGAG ACAGAACCTT 
CAAAGCGTCA AATCAAACCG ATCCACCATA TACATCCAGC TCCACGCCAA CAACCACGAG 
AGTCTGAAAT ACCTGCCTGG AGATCACTTG GGCATCTACC CTGGCAACTA CGAGGACCTG 
GTGACGGCTC TCATAGACAA GCTGGAGGAT GCGCCGCCTG CCAATCAGAT CGTGAGGGTG 
GAGTTTCTGG AGGAGAGGAA CACAGCGCTG GGTGTGATCA GTAACTGGAC GGATGAGAGT 
CGTATTCCTC CCTGCACCAT CCATCAGGCC TTTAAATACT TTCTGGACAT CACGACTCCA 
CCCAGTCCAC TCCTGCTCCA ACAGTTCGCT CCTCTCGCCA CCAACGAGAA ACAGAGGAAG 
AGACTGGAGG TGCTCAGCAA GGGTCTGCAG GAGTATGAGG AGTGGAAGTG GTATAATAAC 
CCCACCATGG TGGAGGTGTT GGAGGAGTTC CCGTCGCTGC AGGTTCCCTG CACCCTGCTC 
CTCACCCAGC TCCCCCTGCT CCAGCCCCGC TACTACTCCA TCAGCTCCTC CCCAGACCTC 
CATCCAGGAG AGATCCACCT CACCGTGGCT GTGGTCTCCT ACCGTACCAG AGATGGTGCA 
GGTCCCGTAC ACCACGGGGT GTGTTCCTCC TGGCTCAACA GTGTGGAAGC GGGAGACATG 
GTGCCATGCT TCGTCAGACG AGCTCCATCA TTCCGGATGC CGAAGGACAG TAGAGTTCCC 
TGTATTCTGG TCGGGCCGGG AACAGGAATC GCTCCATTCA GAAGCTTCTG GCAACAGAGA 
CTCTACGACC TGGAAAATAA AGGGATAAAG GCCTGTCCGA TGATTTTAGT GTTCGGGTGT 
CGACAGTCTC AAATGGACCA CATCTACAAA GAAGAGACCA TCCAGGCCAA GAACAAGGAG 
GTGTTTAAGG AGCTCTACAC CGCTTACTCT CGAGAACCTG GAAGACCAAA GAAATATGTT 
CAGGATGTTC TGCGGGAGCA GTTATCTGAG ACGGTGTATA GGTGTTTGAG GGAGGAGGGG 
GGTCATATCT ACGTCTGTGG GGACGTCACC ATGGCCGGAG ACGTGCTGAA GACCGTCCAG 
CAGATCGTCA AACTGCAAGG CAACATGTCC CTGGAGGACG CGGGATTCTA CATCAGCAAA 
CTGAGGGATG AGAATCGCTA CCATGAAGAC ATTTTCGGCG TGACTCTACG TACGTACGAG 
GTCACGAACC GGCTGCGGTC AGAATCCATC GCATACATCG AGGAAAGCAA GAAAGACTCA 
GACGAGGTGT TTTGTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_015604434.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015459920.1 Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.

Price & Availability↑

CloneID	OAs14487
Clone ID Related Accession (Same CDS sequence)	XM_015604434.1
Accession Version	XM_015604434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	nitric oxide synthase, brain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006738796.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGACGGATT CCGAGCCCAG CGTATGCCAG CTCCAGCCCA ACATCATTTC TGTGCGTCTC 
TTCAAGCGCA AGATTGGTGG ACTGGGCTTC CTGGTGAAGC AGAGGGTCTG CAAACCCCCC 
GTCATCATCT CGGACCTGAT CCGGGGCGGA GCGGCGGAAG AGTGTGGCCT AGTGCAGGTG 
GGCGACATCG TGCTGGCCGT CAACAACAAG CCGCTGGTGG ACCTGAGCTA CGAGCGAGCG 
CTGGAGACCC TGAAGAACGT CTCACCCGAA AGCCACGCTG TGCTGATCCT GCGGGGTCCC 
GAGGGCTTCA CCACACACCT GGAGACCACC CTGAGCGGAG ACGGGAGACC CAGGACCGTC 
CGCATCACCA AACCCGTGTC CAGGCCCTGT GAACGGTGCT CGCCCCTGCA GCCGGGAACA 
CAGGCCAAAG AGCTGAGGGC CATTGAGAAC CTGTCGTCAG CTTCCACCCC CAAAAAAGAG 
GCCAAAGATG CCAAGAACTC CCTGATTGGA CAGGACGCCC TGCTGAGGGA GGGGGGTGGC 
GGCCTGCTGC TGAACGGAGT GGACGATGGG AATGACCTGC TAAAGGAGAT CGAGCCGGTT 
CTGAAGCTGA TCAAGAACAG CAACAGCAAG AAGGAGGTGA ATGGAGACGG ACAGAGAGGG 
GTGGAGAAGA AGGACGTGGA AATACAAGTA AGCTTTGGGG CTGGGTTGGG TTGGGTTGAG 
TTTGAGTGGT TTGTTTTGGG TTGGGCTTGG CCACCGTCAC AAGGCAGATC ATCACCAACC 
AAGACTGTAC AAAATGGAAG TCCAACCAAA TGCCCCAGAT TCATGAGGGT AAAGAACTGG 
GAGAGTGGGG CGGTGTTAAA CGATACCCTA CACCACAACT CCAGCAAGGT GCCAATTTGC 
AACGAGAGGG TGTGTATAGG CTCTATTATG ACGCCGAGCC AGAACCGAAA ACCAGACGAA 
GTCCGAACCA AAGAAGAGCT GCTGCCGCTG GCCACAGACT TCATCAACCA GTACTACACT 
TCAATCAAAA GGTGTGGATC TAAGGCGCAT GTGGATCGAC TGGAAGAGGT GAAGAAAGAG 
ATTGAAACTT CTGGAACGTA CCAGCTCAAA GACACAGAGC TGATATATGG AGCCAAACAT 
GCATGGAGGA ATGCTGCCCG CTGTGTGGGA AGAATCCAGT GGTCCAAACT ACAGGTGTTT 
GATGCCCGAG ACTGCACCAC AGCACACGGA ATGTTTAACT ACATCTGCAA TCACATCAAA 
TACGCCACTA ACAAGGGCAA CCTCAGGTAC GCGCTGGAGC GGATCTGTAT ACAGCAGGGA 
TGGAAGGCTC CTAAAGGGCG TTTCGATGTC CTGCCTCTTC TGCTCCAGGC CAACGGCAAT 
GACCCAGAGC TGTTCGAGAT TCCAGATGAC CTCGTGCTGG AGGTCAACAT CACACACCCC 
AACTCTCCGA GTCTCGGAGG CTGGCAGATC CATAGCAACA AGAGTGCCAT GCTGAGCACA 
AGCGCCCTGC ATGCACAAGT GAGGGACTCG GGCCAAGCCA ACATGAAACT GGCTCCTTCC 
CCAGATCCTA GATCAGTGGC GGAAGTGGCC ACCATGATGG GGCTGGACAC CAGGAAAACG 
TCCTCGCTGT GGAAGGACCA GGCTCTGGTA GAGATCAACA TCGCTGTGCT TTACAGTTAC 
CAGGTCTCTA AGGTGACCAT CGTGGATCAT CACTCAGCTA CAGAGTCGTT TATTAAGCAC 
ATGGAGAACG AGTACAGGGT ACGGGGCGGA TGCCCGGGTG ACTGGGTGTG GATCGTTCCT 
CCAATGTCTG GAAGCATCAC GCCTGTCTTC CACCAGGAGA TGCTCAACTA CAGACTCACT 
CCGTCCTTTG AGTATCAGCC TGATCCGTGG AACACTCATG TGTGGAAAGG TGTGAACGGA 
ACGCCCACCA AGAAACGAGC CATCGGCTTC AAGAAACTTG CGAAATTGTT AATAAGGATG 
ATCTTTTCTC AGGTCATGTC GATGGAGGAG TATGACGTGG TGGATCTGGA GCACGAGACG 
CTGGTGTTGG CGGTCACCAG CACGTTCGGA AACGGAGACC CACCGGAGAA CGGCGAGAAA 
TTCGGTGCAG CGCTGATGGA GATGAGGCAC CCCACCACCA GCGCAGAGGA CAGAAAGAGT 
TACAAGGTTC GCTTTAACAG CGTCTCCTCG TACTCCGACA CCCGGAAGTC CTCCAGCGAT 
GAACCCGAGT CTAAAGCCAA CTTCGAGAGC ACTGGACCTC TCGCTAACGT CAGGTTCTCA 
GTGTTTGGCC TTGGCTCGCG GGCCTACCCA CACTTCTGCG CCTTTGCCCA CGCAGTGGAC 
ACGCTGTTCG AGGAGCTCGG GGGGGAGCGC ATCCTCCGCA TGGGCGAGGG AGACGAACTC 
TGCGGCCAGG AGGAGTCCTT CAGAACCTGG GCCAAGAAAG TTTTTAAGAG AAGGGCTCTC 
TACAACATCC ACAAGAAGAA GGTGTATGGC GCCAAAGTCC TGATGAGACA GAACCTTCAA 
AGCGTCAAAT CAAACCGATC CACCATATAC ATCCAGCTCC ACGCCAACAA CCACGAGAGT 
CTGAAATACC TGCCTGGAGA CCACTTGGGC ATCTACCCTG GCAACCACGA GGACCTGGTG 
ACGGCTCTCA TAGACAAGCT GGAGGATGCG CCGCCTGCCG ATCAGATCGT GAGGGTGGAG 
TTTCTGGAGG AGAGGAACAC AGCGCTGGGT GTGATCAGTA ACTGGACGGA TGAGAGTCGT 
ATTCCTCCCT GCACCATCCA TCAGGCCTTT AAATACTTTC TGGACATCAC GACTCCACCC 
AGTCCACTCC TGCTCCAACA GTTCGCTCCT CTCGCCACCA ACGAGAAACA GAGGAAGAGA 
CTGGAGGTGC TCAGCAAGGG TCTGCAGGAG TATGAGGAGT GGAAGTGGTA TAATAACCCC 
ACCATGGTGG AGGTGTTGGA GGAGTTCCCG TCGCTGCAGG TTCCCTGCAC CCTGCTCCTC 
ACCCAGCTCC CCCTGCTCCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACCTCCAT 
CCAGGAGAGA TCCACCTCAC CGTGGCTGTG GTCTCCTACC GTACCAGAGA TGGTGCAGGT 
CCCGTACACC ACGGGGTGTG TTCCTCCTGG CTCAACAGTG TGGAAGCGGG AGACATGGTG 
CCATGCTTCG TCAGACGAGC TCCATCGTTC CGGATGCCGA AGGACAGTAG AGTTCCCTGT 
ATTCTGGTCG GGCCGGGAAC AGGAATCGCT CCATTCAGAA GCTTCTGGCA ACAGAGACTC 
TACGACCTGG AAAATAAAGG GATAAAGGCC TGTCCGATGA TTTTAGTGTT CGGGTGTCGA 
CAGTCTCAAA TGGACCACAT CTACAAAGAA GAGACCATCC AGGCCAAGAA CAAGGAGGTG 
TTTAAGGAGC TCTACACCGC TTACTCTCGA GAACCTGGAA GACCAAAGAA ATATGTTCAG 
GATGTTCTGC GGGAGCAGTT ATCTGAGACG GTGTATAGGT GTTTGAGGGA GGAGGGGGGT 
CATATCTACG TCTGTGGGGA CGTCACCATG GCCGGAGACG TGCTGAAGAC CGTCCAGCAG 
ATCGTCAAAC TGCACGGCAA CATGTCCCTG GAGGACGCGG GATTCTACAT CAGCAAACTG 
AGGGATGAGA ATCGCTACCA TGAAGACATT TTCGGCGTGA CTCTACGTAC GTACGAGGTC 
ACGAACCGGC TGCGGTCAGA ATCCATCGCA TACATCGAGG AAAGCAAGAA AGACTCAGAC 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015459920.1
CDS	1..3786
Translation	MTDSEPSVCQLQPNIISVRLFKRKIGGLGFLVKQRVCKPPVIISDLIRGGAAEECGLVQVGDIVLAVNNKPLVDLSYERALETLKNVSPESHAVLILRGPEGFTTHLETTLSGDGRPRTVRITKPVSRPCERCSPLQPGTQAKELRAIENLSSASTPKKEAKDAKNSLIGQDALLREGGGGLLLNGVDDGNDLLKEIEPVLKLIKNSNSKKEVNGDGQRGVEKKDVEIQVSFGAGLGWVEFEWFVLGWAWPPSQGRSSPTKTVQNGSPTKCPRFMRVKNWESGAVLNDTLHHNSSKVPICNERVCIGSIMTPSQNRKPDEVRTKEELLPLATDFINQYYTSIKRCGSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRYALERICIQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVNITHPNSPSLGGWQIHSNKSAMLSTSALHAQVRDSGQANMKLAPSPDPRSVAEVATMMGLDTRKTSSLWKDQALVEINIAVLYSYQVSKVTIVDHHSATESFIKHMENEYRVRGGCPGDWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKKRAIGFKKLAKLLIRMIFSQVMSMEEYDVVDLEHETLVLAVTSTFGNGDPPENGEKFGAALMEMRHPTTSAEDRKSYKVRFNSVSSYSDTRKSSSDEPESKANFESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGGERILRMGEGDELCGQEESFRTWAKKVFKRRALYNIHKKKVYGAKVLMRQNLQSVKSNRSTIYIQLHANNHESLKYLPGDHLGIYPGNHEDLVTALIDKLEDAPPADQIVRVEFLEERNTALGVISNWTDESRIPPCTIHQAFKYFLDITTPPSPLLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQVPCTLLLTQLPLLQPRYYSISSSPDLHPGEIHLTVAVVSYRTRDGAGPVHHGVCSSWLNSVEAGDMVPCFVRRAPSFRMPKDSRVPCILVGPGTGIAPFRSFWQQRLYDLENKGIKACPMILVFGCRQSQMDHIYKEETIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYRCLREEGGHIYVCGDVTMAGDVLKTVQQIVKLHGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTYEVTNRLRSESIAYIEESKKDSDE

Target ORF information:

RefSeq Version	XM_015604434.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus nitric oxide synthase 1 (nos1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015604434.1

ORF Insert Sequence:

ATGACGGATT CCGAGCCCAG CGTATGCCAG CTCCAGCCCA ACATCATTTC TGTGCGTCTC 
TTCAAGCGCA AGATTGGTGG ACTGGGCTTC CTGGTGAAGC AGAGGGTCTG CAAACCCCCC 
GTCATCATCT CGGACCTGAT CCGGGGCGGA GCGGCGGAAG AGTGTGGCCT AGTGCAGGTG 
GGCGACATCG TGCTGGCCGT CAACAACAAG CCGCTGGTGG ACCTGAGCTA CGAGCGAGCG 
CTGGAGACCC TGAAGAACGT CTCACCCGAA AGCCACGCTG TGCTGATCCT GCGGGGTCCC 
GAGGGCTTCA CCACACACCT GGAGACCACC CTGAGCGGAG ACGGGAGACC CAGGACCGTC 
CGCATCACCA AACCCGTGTC CAGGCCCTGT GAACGGTGCT CGCCCCTGCA GCCGGGAACA 
CAGGCCAAAG AGCTGAGGGC CATTGAGAAC CTGTCGTCAG CTTCCACCCC CAAAAAAGAG 
GCCAAAGATG CCAAGAACTC CCTGATTGGA CAGGACGCCC TGCTGAGGGA GGGGGGTGGC 
GGCCTGCTGC TGAACGGAGT GGACGATGGG AATGACCTGC TAAAGGAGAT CGAGCCGGTT 
CTGAAGCTGA TCAAGAACAG CAACAGCAAG AAGGAGGTGA ATGGAGACGG ACAGAGAGGG 
GTGGAGAAGA AGGACGTGGA AATACAAGTA AGCTTTGGGG CTGGGTTGGG TTGGGTTGAG 
TTTGAGTGGT TTGTTTTGGG TTGGGCTTGG CCACCGTCAC AAGGCAGATC ATCACCAACC 
AAGACTGTAC AAAATGGAAG TCCAACCAAA TGCCCCAGAT TCATGAGGGT AAAGAACTGG 
GAGAGTGGGG CGGTGTTAAA CGATACCCTA CACCACAACT CCAGCAAGGT GCCAATTTGC 
AACGAGAGGG TGTGTATAGG CTCTATTATG ACGCCGAGCC AGAACCGAAA ACCAGACGAA 
GTCCGAACCA AAGAAGAGCT GCTGCCGCTG GCCACAGACT TCATCAACCA GTACTACACT 
TCAATCAAAA GGTGTGGATC TAAGGCGCAT GTGGATCGAC TGGAAGAGGT GAAGAAAGAG 
ATTGAAACTT CTGGAACGTA CCAGCTCAAA GACACAGAGC TGATATATGG AGCCAAACAT 
GCATGGAGGA ATGCTGCCCG CTGTGTGGGA AGAATCCAGT GGTCCAAACT ACAGGTGTTT 
GATGCCCGAG ACTGCACCAC AGCACACGGA ATGTTTAACT ACATCTGCAA TCACATCAAA 
TACGCCACTA ACAAGGGCAA CCTCAGGTAC GCGCTGGAGC GGATCTGTAT ACAGCAGGGA 
TGGAAGGCTC CTAAAGGGCG TTTCGATGTC CTGCCTCTTC TGCTCCAGGC CAACGGCAAT 
GACCCAGAGC TGTTCGAGAT TCCAGATGAC CTCGTGCTGG AGGTCAACAT CACACACCCC 
AACTCTCCGA GTCTCGGAGG CTGGCAGATC CATAGCAACA AGAGTGCCAT GCTGAGCACA 
AGCGCCCTGC ATGCACAAGT GAGGGACTCG GGCCAAGCCA ACATGAAACT GGCTCCTTCC 
CCAGATCCTA GATCAGTGGC GGAAGTGGCC ACCATGATGG GGCTGGACAC CAGGAAAACG 
TCCTCGCTGT GGAAGGACCA GGCTCTGGTA GAGATCAACA TCGCTGTGCT TTACAGTTAC 
CAGGTCTCTA AGGTGACCAT CGTGGATCAT CACTCAGCTA CAGAGTCGTT TATTAAGCAC 
ATGGAGAACG AGTACAGGGT ACGGGGCGGA TGCCCGGGTG ACTGGGTGTG GATCGTTCCT 
CCAATGTCTG GAAGCATCAC GCCTGTCTTC CACCAGGAGA TGCTCAACTA CAGACTCACT 
CCGTCCTTTG AGTATCAGCC TGATCCGTGG AACACTCATG TGTGGAAAGG TGTGAACGGA 
ACGCCCACCA AGAAACGAGC CATCGGCTTC AAGAAACTTG CGAAATTGTT AATAAGGATG 
ATCTTTTCTC AGGTCATGTC GATGGAGGAG TATGACGTGG TGGATCTGGA GCACGAGACG 
CTGGTGTTGG CGGTCACCAG CACGTTCGGA AACGGAGACC CACCGGAGAA CGGCGAGAAA 
TTCGGTGCAG CGCTGATGGA GATGAGGCAC CCCACCACCA GCGCAGAGGA CAGAAAGAGT 
TACAAGGTTC GCTTTAACAG CGTCTCCTCG TACTCCGACA CCCGGAAGTC CTCCAGCGAT 
GAACCCGAGT CTAAAGCCAA CTTCGAGAGC ACTGGACCTC TCGCTAACGT CAGGTTCTCA 
GTGTTTGGCC TTGGCTCGCG GGCCTACCCA CACTTCTGCG CCTTTGCCCA CGCAGTGGAC 
ACGCTGTTCG AGGAGCTCGG GGGGGAGCGC ATCCTCCGCA TGGGCGAGGG AGACGAACTC 
TGCGGCCAGG AGGAGTCCTT CAGAACCTGG GCCAAGAAAG TTTTTAAGAG AAGGGCTCTC 
TACAACATCC ACAAGAAGAA GGTGTATGGC GCCAAAGTCC TGATGAGACA GAACCTTCAA 
AGCGTCAAAT CAAACCGATC CACCATATAC ATCCAGCTCC ACGCCAACAA CCACGAGAGT 
CTGAAATACC TGCCTGGAGA CCACTTGGGC ATCTACCCTG GCAACCACGA GGACCTGGTG 
ACGGCTCTCA TAGACAAGCT GGAGGATGCG CCGCCTGCCG ATCAGATCGT GAGGGTGGAG 
TTTCTGGAGG AGAGGAACAC AGCGCTGGGT GTGATCAGTA ACTGGACGGA TGAGAGTCGT 
ATTCCTCCCT GCACCATCCA TCAGGCCTTT AAATACTTTC TGGACATCAC GACTCCACCC 
AGTCCACTCC TGCTCCAACA GTTCGCTCCT CTCGCCACCA ACGAGAAACA GAGGAAGAGA 
CTGGAGGTGC TCAGCAAGGG TCTGCAGGAG TATGAGGAGT GGAAGTGGTA TAATAACCCC 
ACCATGGTGG AGGTGTTGGA GGAGTTCCCG TCGCTGCAGG TTCCCTGCAC CCTGCTCCTC 
ACCCAGCTCC CCCTGCTCCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACCTCCAT 
CCAGGAGAGA TCCACCTCAC CGTGGCTGTG GTCTCCTACC GTACCAGAGA TGGTGCAGGT 
CCCGTACACC ACGGGGTGTG TTCCTCCTGG CTCAACAGTG TGGAAGCGGG AGACATGGTG 
CCATGCTTCG TCAGACGAGC TCCATCGTTC CGGATGCCGA AGGACAGTAG AGTTCCCTGT 
ATTCTGGTCG GGCCGGGAAC AGGAATCGCT CCATTCAGAA GCTTCTGGCA ACAGAGACTC 
TACGACCTGG AAAATAAAGG GATAAAGGCC TGTCCGATGA TTTTAGTGTT CGGGTGTCGA 
CAGTCTCAAA TGGACCACAT CTACAAAGAA GAGACCATCC AGGCCAAGAA CAAGGAGGTG 
TTTAAGGAGC TCTACACCGC TTACTCTCGA GAACCTGGAA GACCAAAGAA ATATGTTCAG 
GATGTTCTGC GGGAGCAGTT ATCTGAGACG GTGTATAGGT GTTTGAGGGA GGAGGGGGGT 
CATATCTACG TCTGTGGGGA CGTCACCATG GCCGGAGACG TGCTGAAGAC CGTCCAGCAG 
ATCGTCAAAC TGCACGGCAA CATGTCCCTG GAGGACGCGG GATTCTACAT CAGCAAACTG 
AGGGATGAGA ATCGCTACCA TGAAGACATT TTCGGCGTGA CTCTACGTAC GTACGAGGTC 
ACGAACCGGC TGCGGTCAGA ATCCATCGCA TACATCGAGG AAAGCAAGAA AGACTCAGAC 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nos1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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