LOC5579840 gene cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

mRNA	Protein	Name
XM_001651466.1	XP_001651516.1	AAEL005843-PB
XM_021855631.1	XP_021711323.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855626.1	XP_021711318.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855628.1	XP_021711320.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855629.1	XP_021711321.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_001651466.2	XP_001651516.2	mediator of RNA polymerase II transcription subunit 12 isoform X4
XM_021855632.1	XP_021711324.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855636.1	XP_021711328.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855638.1	XP_021711330.1	protein split ends isoform X3
XM_001651465.1	XP_001651515.1	AAEL005843-PA
XM_001651465.2	XP_001651515.2	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855635.1	XP_021711327.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855637.1	XP_021711329.1	protein split ends isoform X2
XM_021855627.1	XP_021711319.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_021855630.1	XP_021711322.1	mediator of RNA polymerase II transcription subunit 12 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe13980	XM_001651466.1 Latest version!	Aedes aegypti AAEL005843-RB mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAe13981	XM_001651465.1 Latest version!	Aedes aegypti AAEL005843-RA mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAe130012	XM_001651465.2 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855631.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855635.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855632.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855627.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855626.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855636.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855629.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855630.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130012	XM_021855628.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe130013	XM_021855637.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAe130014	XM_021855638.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAe130015	XM_001651466.2 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

LOC5579840 ( XM_001651466.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001651516.1 Aedes aegypti AAEL005843-RB mRNA.

Price & Availability↑

CloneID	OAe13980
Clone ID Related Accession (Same CDS sequence)	XM_001651466.1
Accession Version	XM_001651466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL005843-PB
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810616).

ATGTATCCGA GTATTACTTC ACAGTTTCGA ATGGGCGGTG CCGTCCAAAG TCCGTACAAT 
CCTTCTCCGT CGCAACAGAT GAACAATCCA AGTACAATGC TGATCTCTTC GAGCACAAAC 
TCGCTCATGT CGTCGTCGGT AAAACCGTCA TCCCAACAAA TCGGTGCAAT AGGTTCTAAG 
AGTGGATCAG TTGGACAACC TTATGGCCAA CAATACATGG GGATGTTCCA ACCCGCTCCA 
CCACTGCAGA ACAATAGCTT CTACTCAAAT TCCGCTGGAG GCCAGAGTGC ATTTTTCAGC 
GGTGCAGGAG CTACACAAAA TTACGGAATA CCGGCAGCTG CTGCTGGAAT GTTCGGTGGA 
CACGGTGGCC AAACCCCATC GAACGCTCCG CCTCCGCAAC AGCAACTTCC GAACTATCCC 
AACGCATCGC AGTTCTTAAA TTCACCGTTA GCGTTGGCGG CAAACGCTAT TTCTCAGCAA 
TTCCGCGGAG GGCCCACAAC GAATCCGCAA TCTGCCGCTG CAGCCGCAGC ATACATGAAA 
GGAAACCAAC CCCAATCGCA TATGCAGGAT CCCAATGGTC GGCAGCTGAA AAGCCCTTTG 
AATTCTGATG CAATGAATAA TTTAAAACAG ATTCCACCCC AAGCGAGTCC GCCACATCAT 
AAGGGTTACG CAACAGCATG GGAGATACAA AATCAACTGA TTCAACAACA ACAGCAGCAG 
CAAAACCAGC AGTCTAACCA ACAGCAGCCA CCACAACAGC AAAATCCACA ACAACATCCC 
CAACAGCAAA TGAGTCAACC ACCGCAGTCT CAGCAGCCCA ATCCAAATCG AAATATGATG 
CCCCCCAACC AGCAACCTCC AGCAGGACTG GGAGGTGCCG GTGGGGGTCG TCCTCCACAG 
GGTGTCCAGG GTGGGGGCAT GCCTCAGCGA TACCCAGCTC CCATCCAACG ACCGACTAAC 
TACTCCCAGG CGCCAGTACC GGGAATGCAT CAGCAACGGC CCATTCGTCA GTCAGGCCAT 
AACCCTGGTC AACAACAACA GGCAAACATG GCACAGGCAA TGGGCAATCC CAACAAACAT 
TACTACGGTG GGAATCGAGG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651516.1
CDS	20..1123
Translation	MYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRG

Target ORF information:

RefSeq Version	XM_001651466.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL005843-RB mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651466.1

ORF Insert Sequence:

ATGTATCCGA GTATTACTTC ACAGTTTCGA ATGGGCGGTG CCGTCCAAAG TCCGTACAAT 
CCTTCTCCGT CGCAACAGAT GAACAATCCA AGTACAATGC TGATCTCTTC GAGCACAAAC 
TCGCTCATGT CGTCGTCGGT AAAACCGTCA TCCCAACAAA TCGGTGCAAT AGGTTCTAAG 
AGTGGATCAG TTGGACAACC TTATGGCCAA CAATACATGG GGATGTTCCA ACCCGCTCCA 
CCACTGCAGA ACAATAGCTT CTACTCAAAT TCCGCTGGAG GCCAGAGTGC ATTTTTCAGC 
GGTGCAGGAG CTACACAAAA TTACGGAATA CCGGCAGCTG CTGCTGGAAT GTTCGGTGGA 
CACGGTGGCC AAACCCCATC GAACGCTCCG CCTCCGCAAC AGCAACTTCC GAACTATCCC 
AACGCATCGC AGTTCTTAAA TTCACCGTTA GCGTTGGCGG CAAACGCTAT TTCTCAGCAA 
TTCCGCGGAG GGCCCACAAC GAATCCGCAA TCTGCCGCTG CAGCCGCAGC ATACATGAAA 
GGAAACCAAC CCCAATCGCA TATGCAGGAT CCCAATGGTC GGCAGCTGAA AAGCCCTTTG 
AATTCTGATG CAATGAATAA TTTAAAACAG ATTCCACCCC AAGCGAGTCC GCCACATCAT 
AAGGGTTACG CAACAGCATG GGAGATACAA AATCAACTGA TTCAACAACA ACAGCAGCAG 
CAAAACCAGC AGTCTAACCA ACAGCAGCCA CCACAACAGC AAAATCCACA ACAACATCCC 
CAACAGCAAA TGAGTCAACC ACCGCAGTCT CAGCAGCCCA ATCCAAATCG AAATATGATG 
CCCCCCAACC AGCAACCTCC AGCAGGACTG GGAGGTGCCG GTGGGGGTCG TCCTCCACAG 
GGTGTCCAGG GTGGGGGCAT GCCTCAGCGA TACCCAGCTC CCATCCAACG ACCGACTAAC 
TACTCCCAGG CGCCAGTACC GGGAATGCAT CAGCAACGGC CCATTCGTCA GTCAGGCCAT 
AACCCTGGTC AACAACAACA GGCAAACATG GCACAGGCAA TGGGCAATCC CAACAAACAT 
TACTACGGTG GGAATCGAGG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_001651465.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001651515.1 Aedes aegypti AAEL005843-RA mRNA.

Price & Availability↑

CloneID	OAe13981
Clone ID Related Accession (Same CDS sequence)	XM_001651465.1
Accession Version	XM_001651465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL005843-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810616).

ATGTATCCGA GTATTACTTC ACAGTTTCGA ATGGGCGGTG CCGTCCAAAG TCCGTACAAT 
CCTTCTCCGT CGCAACAGAT GAACAATCCA AGTACAATGC TGATCTCTTC GAGCACAAAC 
TCGCTCATGT CGTCGTCGGT AAAACCGTCA TCCCAACAAA TCGGTGCAAT AGGTTCTAAG 
AGTGGATCAG TTGGACAACC TTATGGCCAA CAATACATGG GGATGTTCCA ACCCGCTCCA 
CCACTGCAGA ACAATAGCTT CTACTCAAAT TCCGCTGGAG GCCAGAGTGC ATTTTTCAGC 
GGTGCAGGAG CTACACAAAA TTACGGAATA CCGGCAGCTG CTGCTGGAAT GTTCGGTGGA 
CACGGTGGCC AAACCCCATC GAACGCTCCG CCTCCGCAAC AGCAACTTCC GAACTATCCC 
AACGCATCGC AGTTCTTAAA TTCACCGTTA GCGTTGGCGG CAAACGCTAT TTCTCAGCAA 
TTCCGCGGAG GGCCCACAAC GAATCCGCAA TCTGCCGCTG CAGCCGCAGC ATACATGAAA 
GGAAACCAAC CCCAATCGCA TATGCAGGAT CCCAATGGTC GGCAGCTGAA AAGCCCTTTG 
AATTCTGATG CAATGAATAA TTTAAAACAG ATTCCACCCC AAGCGAGTCC GCCACATCAT 
AAGGGTTACG CAACAGCATG GGAGATACAA AATCAACTGA TTCAACAACA ACAGCAGCAG 
CAAAACCAGC AGTCTAACCA ACAGCAGCCA CCACAACAGC AAAATCCACA ACAACATCCC 
CAACAGCAAA TGAGTCAACC ACCGCAGTCT CAGCAGCCCA ATCCAAATCG AAATATGATG 
CCCCCCAACC AGCAACCTCC AGCAGGACTG GGAGGTGCCG GTGGGGGTCG TCCTCCACAG 
GGTGTCCAGG GTGGGGGCAT GCCTCAGCGA TACCCAGCTC CCATCCAACG ACCGACTAAC 
TACTCCCAGG CGCCAGTACC GGGAATGCAT CAGCAACGGC CCATTCGTCA GTCAGGCCAT 
AACCCTGGTC AACAACAACA GGCAAACATG GCACAGGCAA TGGGCAATCC CAACAAACAT 
TACTACGGTG GGAATCGAGG TGGCGGTCAC GTTAACGCGA TGAACGAGCA AAGCCTTGGC 
GGAAAACCTG GCGGTGGAAA TGAGAAACCA GTGCTCGACG GAGATGTTAA CAAGGATGAT 
TCCAAGAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651515.1
CDS	20..1234
Translation	MYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_001651465.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL005843-RA mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651465.1

ORF Insert Sequence:

ATGTATCCGA GTATTACTTC ACAGTTTCGA ATGGGCGGTG CCGTCCAAAG TCCGTACAAT 
CCTTCTCCGT CGCAACAGAT GAACAATCCA AGTACAATGC TGATCTCTTC GAGCACAAAC 
TCGCTCATGT CGTCGTCGGT AAAACCGTCA TCCCAACAAA TCGGTGCAAT AGGTTCTAAG 
AGTGGATCAG TTGGACAACC TTATGGCCAA CAATACATGG GGATGTTCCA ACCCGCTCCA 
CCACTGCAGA ACAATAGCTT CTACTCAAAT TCCGCTGGAG GCCAGAGTGC ATTTTTCAGC 
GGTGCAGGAG CTACACAAAA TTACGGAATA CCGGCAGCTG CTGCTGGAAT GTTCGGTGGA 
CACGGTGGCC AAACCCCATC GAACGCTCCG CCTCCGCAAC AGCAACTTCC GAACTATCCC 
AACGCATCGC AGTTCTTAAA TTCACCGTTA GCGTTGGCGG CAAACGCTAT TTCTCAGCAA 
TTCCGCGGAG GGCCCACAAC GAATCCGCAA TCTGCCGCTG CAGCCGCAGC ATACATGAAA 
GGAAACCAAC CCCAATCGCA TATGCAGGAT CCCAATGGTC GGCAGCTGAA AAGCCCTTTG 
AATTCTGATG CAATGAATAA TTTAAAACAG ATTCCACCCC AAGCGAGTCC GCCACATCAT 
AAGGGTTACG CAACAGCATG GGAGATACAA AATCAACTGA TTCAACAACA ACAGCAGCAG 
CAAAACCAGC AGTCTAACCA ACAGCAGCCA CCACAACAGC AAAATCCACA ACAACATCCC 
CAACAGCAAA TGAGTCAACC ACCGCAGTCT CAGCAGCCCA ATCCAAATCG AAATATGATG 
CCCCCCAACC AGCAACCTCC AGCAGGACTG GGAGGTGCCG GTGGGGGTCG TCCTCCACAG 
GGTGTCCAGG GTGGGGGCAT GCCTCAGCGA TACCCAGCTC CCATCCAACG ACCGACTAAC 
TACTCCCAGG CGCCAGTACC GGGAATGCAT CAGCAACGGC CCATTCGTCA GTCAGGCCAT 
AACCCTGGTC AACAACAACA GGCAAACATG GCACAGGCAA TGGGCAATCC CAACAAACAT 
TACTACGGTG GGAATCGAGG TGGCGGTCAC GTTAACGCGA TGAACGAGCA AAGCCTTGGC 
GGAAAACCTG GCGGTGGAAA TGAGAAACCA GTGCTCGACG GAGATGTTAA CAAGGATGAT 
TCCAAGAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855631.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711323.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711323.1
CDS	692..7708
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855631.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855631.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855626.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711318.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X2, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711318.1
CDS	854..7870
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855626.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855626.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855628.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711320.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X4, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711320.1
CDS	726..7742
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855628.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855628.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855629.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711321.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X5, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711321.1
CDS	824..7840
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855629.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855629.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855632.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711324.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X8, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711324.1
CDS	703..7719
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855632.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855632.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855636.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711328.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X10, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711328.1
CDS	467..7483
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855636.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855636.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_001651465.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001651515.2 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X1, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_001651465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001651465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651515.2
CDS	899..7915
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_001651465.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651465.2

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855635.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711327.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X9, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711327.1
CDS	879..7895
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855635.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855635.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855627.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711319.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X3, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711319.1
CDS	774..7790
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855627.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855627.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855630.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711322.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X6, mRNA.

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CloneID	OAe130012
Clone ID Related Accession (Same CDS sequence)	XM_021855631.1 , XM_021855626.1 , XM_021855628.1 , XM_021855629.1 , XM_021855632.1 , XM_021855636.1 , XM_001651465.2 , XM_021855635.1 , XM_021855627.1 , XM_021855630.1
Accession Version	XM_021855630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711322.1
CDS	794..7810
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855630.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855630.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTGGCGGTC ACGTTAACGC GATGAACGAG CAAAGCCTTG GCGGAAAACC TGGCGGTGGA 
AATGAGAAAC CAGTGCTCGA CGGAGATGTT AACAAGGATG ATTCCAAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855637.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711329.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X11, mRNA.

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CloneID	OAe130013
Clone ID Related Accession (Same CDS sequence)	XM_021855637.1
Accession Version	XM_021855637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein split ends isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCATTCC ACCCCAAGCG AGTCCGCCAC ATCATAAGGG TTACGCAACA 
GCATGGGAGA TACAAAATCA ACTGATTCAA CAACAACAGC AGCAGCAAAA CCAGCAGTCT 
AACCAACAGC AGCCACCACA ACAGCAAAAT CCACAACAAC ATCCCCAACA GCAAATGAGT 
CAACCACCGC AGTCTCAGCA GCCCAATCCA AATCGAAATA TGATGCCCCC CAACCAGCAA 
CCTCCAGCAG GACTGGGAGG TGCCGGTGGG GGTCGTCCTC CACAGGGTGT CCAGGGTGGG 
GGCATGCCTC AGCGATACCC AGCTCCCATC CAACGACCGA CTAACTACTC CCAGGCGCCA 
GTACCGGGAA TGCATCAGCA ACGGCCCATT CGTCAGTCAG GCCATAACCC TGGTCAACAA 
CAACAGGCAA ACATGGCACA GGCAATGGGC AATCCCAACA AACATTACTA CGGTGGGAAT 
CGAGGTGGCG GTCACGTTAA CGCGATGAAC GAGCAAAGCC TTGGCGGAAA ACCTGGCGGT 
GGAAATGAGA AACCAGTGCT CGACGGAGAT GTTAACAAGG ATGATTCCAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711329.1
CDS	879..7838
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855637.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855637.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCATTCC ACCCCAAGCG AGTCCGCCAC ATCATAAGGG TTACGCAACA 
GCATGGGAGA TACAAAATCA ACTGATTCAA CAACAACAGC AGCAGCAAAA CCAGCAGTCT 
AACCAACAGC AGCCACCACA ACAGCAAAAT CCACAACAAC ATCCCCAACA GCAAATGAGT 
CAACCACCGC AGTCTCAGCA GCCCAATCCA AATCGAAATA TGATGCCCCC CAACCAGCAA 
CCTCCAGCAG GACTGGGAGG TGCCGGTGGG GGTCGTCCTC CACAGGGTGT CCAGGGTGGG 
GGCATGCCTC AGCGATACCC AGCTCCCATC CAACGACCGA CTAACTACTC CCAGGCGCCA 
GTACCGGGAA TGCATCAGCA ACGGCCCATT CGTCAGTCAG GCCATAACCC TGGTCAACAA 
CAACAGGCAA ACATGGCACA GGCAATGGGC AATCCCAACA AACATTACTA CGGTGGGAAT 
CGAGGTGGCG GTCACGTTAA CGCGATGAAC GAGCAAAGCC TTGGCGGAAA ACCTGGCGGT 
GGAAATGAGA AACCAGTGCT CGACGGAGAT GTTAACAAGG ATGATTCCAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_021855638.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711330.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X12, mRNA.

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CloneID	OAe130014
Clone ID Related Accession (Same CDS sequence)	XM_021855638.1
Accession Version	XM_021855638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein split ends isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCTGGGA GATACAAAAT CAACTGATTC AACAACAACA GCAGCAGCAA 
AACCAGCAGT CTAACCAACA GCAGCCACCA CAACAGCAAA ATCCACAACA ACATCCCCAA 
CAGCAAATGA GTCAACCACC GCAGTCTCAG CAGCCCAATC CAAATCGAAA TATGATGCCC 
CCCAACCAGC AACCTCCAGC AGGACTGGGA GGTGCCGGTG GGGGTCGTCC TCCACAGGGT 
GTCCAGGGTG GGGGCATGCC TCAGCGATAC CCAGCTCCCA TCCAACGACC GACTAACTAC 
TCCCAGGCGC CAGTACCGGG AATGCATCAG CAACGGCCCA TTCGTCAGTC AGGCCATAAC 
CCTGGTCAAC AACAACAGGC AAACATGGCA CAGGCAATGG GCAATCCCAA CAAACATTAC 
TACGGTGGGA ATCGAGGTGG CGGTCACGTT AACGCGATGA ACGAGCAAAG CCTTGGCGGA 
AAACCTGGCG GTGGAAATGA GAAACCAGTG CTCGACGGAG ATGTTAACAA GGATGATTCC 
AAGAAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711330.1
CDS	879..7790
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRGGGHVNAMNEQSLGGKPGGGNEKPVLDGDVNKDDSKKK

Target ORF information:

RefSeq Version	XM_021855638.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855638.1

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCTGGGA GATACAAAAT CAACTGATTC AACAACAACA GCAGCAGCAA 
AACCAGCAGT CTAACCAACA GCAGCCACCA CAACAGCAAA ATCCACAACA ACATCCCCAA 
CAGCAAATGA GTCAACCACC GCAGTCTCAG CAGCCCAATC CAAATCGAAA TATGATGCCC 
CCCAACCAGC AACCTCCAGC AGGACTGGGA GGTGCCGGTG GGGGTCGTCC TCCACAGGGT 
GTCCAGGGTG GGGGCATGCC TCAGCGATAC CCAGCTCCCA TCCAACGACC GACTAACTAC 
TCCCAGGCGC CAGTACCGGG AATGCATCAG CAACGGCCCA TTCGTCAGTC AGGCCATAAC 
CCTGGTCAAC AACAACAGGC AAACATGGCA CAGGCAATGG GCAATCCCAA CAAACATTAC 
TACGGTGGGA ATCGAGGTGG CGGTCACGTT AACGCGATGA ACGAGCAAAG CCTTGGCGGA 
AAACCTGGCG GTGGAAATGA GAAACCAGTG CTCGACGGAG ATGTTAACAA GGATGATTCC 
AAGAAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579840 ( XM_001651466.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001651516.2 Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X13, mRNA.

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CloneID	OAe130015
Clone ID Related Accession (Same CDS sequence)	XM_001651466.2
Accession Version	XM_001651466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001651466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651516.2
CDS	879..7784
Translation	MNALGGTRGERNAKPKFAALDINKLYSTSRGESLEPSTQKSAAPRKHGMQSLGKVPSARRPPANLPSLKAEISIPSDQQGTWGSEAGDNQNNNSSITSTSAAPSAAAGSSTNNNATVGAGGVHSTSHASSSQIPHSGASSSSQWSSNEFPSLDGTGQYGPSGKQQHHHLHDGTDGRAMGQYMDGPQVSLRPQTDAASWMQQQQQSSGGGNNAGGMNGPNSNQQGQGPQQQPAAPLPPQFRALMPPFMYRGNGGGGGNSVTPPPSSNEDINRGNTGGGNGGSSGGGMRSNNSNMGYNSMLPSFVAPPTNHHQSHGSNYSPTQSGGRYRDSYSGGRRGQMQPPRLANRQQQHQNDDHRSQTAAPAYVEPDILVQRPIIRDEELQRIEAIAKDEGWAKDDEIDYNQKLQFSDDELEPSPPKEIPMSVVKQEKSPSQIQQQQQQMQQQQLQQQQSQQEDDKRMAWSREVEREQERLEQEQIERLDRERQEKEREQTNMRPVQQNGHIGGRGLDAEAKERLKQRREEDQKREMERKQAAARKLQELEQKLSQKKNEDNVVGSVTSPATTKDGEDRDLVVAGFNESGESKERSSSSVRGGDRERLDSKEYRDRDRDRFEYGRYDRDYRDRDRGERDRTERDRTERARGEQDRERDAWGEVPPYSKNFQSNLPPRFQKRKLERNPSGNSLQGSGSYITRDQNITRNSSTANQGSMMSQSQPQTENKSVPFAQQYDPRFIHSQQNFGKSQSSRRAAPGEDSSRGRDLRDRGDDRDSMTKDREQQRDREMIPREIMKRRIDSEEDDRFSSSGRESSSKLSGGRTTPQLVRSVSDTSQRKTSVSSDDNTRQLDHHHHHNSSREIIGSWESETEKEDRRQAEAAAALAKENQMQQLQLQQQQQPDSRRISESSEGEPKQILQRIKESSPALDEISKGRNDELKTNEPKLLSTAWEESVEAAEEEELKASEASLTSIDDKKDVSTLEVTKTDSGKVIDQENKMSVDTGREMDDSKYGSKKPMHSRGTGRHETRGGGHSRGGFAGGSGFHRGGSSSSWGRRGGPGGNRMNQGRNYNQDYWSESDYSDDGDEEYRKDGKYERKDGMGNQYRNSGSSVGQAQNMKEGFAPRGEPSRRGRGGMSSAMSGPGFRKTTVGKKMDGYGPPSSKSPFGSSEEKTESKNQSVPKDMKPADQTLNADDRTKQKQAALSAGLTNKPVGGPKPPTSGPLPQRSPSGNMKPPGKPAEETLSDQEKLKASLEKKTRNDSKTESHDEKSKEKPRTESESHDTDDASKESLKTGQPRPKQQQPPGIKSVQPSSTVPVGKPSTTIAKPNTVVPSGAPTTAGPKPTTPAAQPVMQQPKPNQDPRGQTNNRSMNITPRMVKPKERREGGFQDGQQSGGETNESEANVVASSQPQPVQEKVNTLNVNSSKTDNSKQHLDGATPPVNTIIFENTNYKSSTVVVSTVPQHMLQPQQIQQPLQIQVQQQQPQGHAPAQIKRQHSGGAAPGQLIALHQQQQQQQQQQQQQQQLQHQQQMVQLVKQEHTVRHPSQEEAFKVAAGVPQSGSMHDIMEQQQQQQQPPQRPQPTNGPQQPQQQQQLQQNQVPLQQQVPQQQQPSSQTDVITSALHNMSFPKPAEADYDKEMKPFTFDADLSQLIEAKNKAAGLCLPKAQNMISPSTAELNMKIASVKKVWEMPSVPEHVTEDPSSAANAAAAAVQLAANFAAVNSQHQQHLHQFQHSHSNLTHAHQAHTLAAAAAAGQSYAGGFGQEQQSLDQHYSKANNDSVVIGGTTGGNDGDNNYNNPQHAVVGQGNNVQHQSAANMNMKHDVLANNVNVCKVKPTQQNMHQSTGLGLSPPPMQQGTIPSAPQTYFQPSQYGMSAIPSPPAVIYNSSAMPSQGGLYNAFQIEAAGRSQFSQFPGHYGTTGTGPYNAYMTTPPNLQTAPTPDMYPSITSQFRMGGAVQSPYNPSPSQQMNNPSTMLISSSTNSLMSSSVKPSSQQIGAIGSKSGSVGQPYGQQYMGMFQPAPPLQNNSFYSNSAGGQSAFFSGAGATQNYGIPAAAAGMFGGHGGQTPSNAPPPQQQLPNYPNASQFLNSPLALAANAISQQFRGGPTTNPQSAAAAAAYMKGNQPQSHMQDPNGRQLKSPLNSDAMNNLKQIPPQASPPHHKGYATAWEIQNQLIQQQQQQQNQQSNQQQPPQQQNPQQHPQQQMSQPPQSQQPNPNRNMMPPNQQPPAGLGGAGGGRPPQGVQGGGMPQRYPAPIQRPTNYSQAPVPGMHQQRPIRQSGHNPGQQQQANMAQAMGNPNKHYYGGNRG

Target ORF information:

RefSeq Version	XM_001651466.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 12 (LOC5579840), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651466.2

ORF Insert Sequence:

ATGAATGCAT TGGGGGGAAC TAGGGGGGAG CGAAATGCAA AGCCCAAATT CGCAGCGTTA 
GATATCAACA AACTCTACAG CACAAGTCGG GGAGAATCTC TTGAACCATC AACTCAGAAA 
AGTGCAGCTC CTCGTAAACA TGGAATGCAA AGTTTGGGAA AGGTTCCCTC GGCTCGTCGA 
CCGCCAGCAA ACCTTCCGTC TCTGAAAGCC GAAATCTCCA TTCCATCGGA TCAGCAAGGC 
ACCTGGGGTA GCGAAGCAGG AGACAACCAA AACAACAACT CTAGTATTAC TTCAACATCA 
GCAGCACCAT CAGCAGCCGC CGGTAGCAGC ACAAATAACA ACGCCACGGT TGGTGCCGGA 
GGTGTGCACA GCACTTCACA CGCCAGCAGT TCACAGATTC CCCACTCGGG CGCATCATCC 
TCTTCACAAT GGAGTTCGAA CGAGTTCCCC TCGCTGGATG GCACTGGTCA GTATGGTCCG 
AGCGGCAAAC AGCAGCATCA TCATCTCCAC GATGGCACAG ACGGCAGAGC GATGGGTCAG 
TACATGGATG GTCCACAAGT CAGTCTGCGG CCTCAAACGG ATGCTGCGAG TTGGATGCAG 
CAACAACAGC AGAGCAGTGG GGGCGGAAAC AACGCCGGAG GTATGAACGG GCCAAACAGC 
AACCAACAGG GCCAGGGCCC CCAACAGCAA CCAGCTGCAC CCCTACCGCC TCAGTTCCGG 
GCACTGATGC CACCGTTCAT GTATCGTGGC AACGGAGGCG GAGGTGGAAA TTCTGTAACG 
CCGCCACCTT CTTCGAACGA GGATATCAAT CGTGGCAATA CTGGAGGCGG CAATGGTGGA 
AGCAGTGGAG GTGGAATGCG AAGCAACAAC AGCAACATGG GCTACAACAG CATGTTGCCA 
AGCTTTGTGG CTCCTCCAAC GAATCATCAT CAATCTCATG GATCAAATTA TTCGCCAACA 
CAGTCGGGTG GTAGATACCG AGACTCGTAT TCAGGCGGAC GCCGTGGACA GATGCAACCG 
CCTCGTCTTG CCAACAGGCA ACAGCAGCAT CAAAACGATG ATCATCGCTC TCAAACGGCT 
GCTCCAGCTT ATGTTGAACC GGACATACTT GTGCAGCGAC CGATTATACG AGATGAAGAA 
CTTCAACGCA TTGAAGCGAT TGCTAAAGAC GAAGGATGGG CCAAGGATGA TGAAATTGAT 
TATAACCAGA AATTACAATT CTCGGATGAC GAATTGGAAC CAAGTCCCCC AAAAGAAATT 
CCAATGTCCG TTGTTAAGCA AGAGAAATCC CCTTCACAGA TACAACAGCA ACAACAGCAA 
ATGCAACAGC AACAATTGCA GCAGCAGCAG TCTCAACAGG AAGATGACAA ACGAATGGCT 
TGGAGTCGAG AAGTGGAACG GGAACAAGAG AGGCTTGAAC AGGAACAAAT TGAACGTCTT 
GATCGTGAGC GCCAGGAAAA AGAAAGAGAG CAGACGAATA TGCGTCCAGT GCAACAAAAT 
GGGCATATAG GAGGACGAGG CTTAGATGCG GAAGCGAAGG AAAGATTGAA GCAACGTCGA 
GAAGAAGATC AGAAACGAGA AATGGAACGG AAACAAGCGG CCGCAAGAAA GCTGCAAGAA 
CTTGAACAAA AGTTAAGTCA GAAGAAGAAC GAAGACAACG TTGTTGGTTC TGTTACTTCA 
CCTGCAACTA CGAAAGATGG CGAAGATCGT GATCTAGTAG TGGCAGGTTT TAACGAATCT 
GGTGAAAGTA AGGAACGCAG CAGTTCAAGT GTTCGTGGCG GAGACCGAGA AAGACTAGAT 
TCGAAAGAAT ATAGAGACCG CGATCGAGAT CGTTTTGAAT ATGGACGATA TGATAGAGAT 
TACCGCGATC GGGACAGAGG AGAGCGGGAT AGGACAGAGC GAGATAGAAC AGAAAGAGCT 
AGAGGCGAAC AGGATCGTGA GCGTGATGCT TGGGGTGAAG TACCGCCCTA CTCTAAAAAT 
TTCCAGTCCA ATTTACCGCC ACGTTTTCAG AAACGTAAAC TTGAACGAAA TCCATCGGGA 
AATAGTTTAC AGGGAAGTGG AAGCTACATT ACTAGAGATC AGAACATCAC TCGCAACAGT 
AGCACTGCCA ATCAAGGATC GATGATGTCG CAGTCACAAC CACAAACTGA GAACAAAAGT 
GTTCCGTTCG CTCAACAGTA TGATCCACGA TTCATCCATA GCCAACAAAA TTTTGGCAAG 
AGTCAATCAT CTAGAAGAGC TGCTCCTGGT GAAGATAGTT CTCGTGGTCG TGATCTTAGG 
GATCGCGGAG ATGACCGTGA CTCGATGACG AAAGACAGAG AGCAACAACG AGATCGTGAG 
ATGATTCCCA GAGAGATTAT GAAACGACGT ATCGACTCAG AAGAAGATGA CCGTTTCAGC 
AGCAGTGGAA GGGAATCATC AAGCAAACTC AGCGGTGGTA GAACTACGCC ACAGCTGGTC 
AGAAGTGTAT CTGATACATC TCAAAGGAAA ACTAGTGTAT CCAGCGATGA TAACACTCGT 
CAACTGGACC ATCACCATCA TCATAACAGT TCGAGAGAAA TCATTGGTTC GTGGGAAAGT 
GAAACGGAGA AAGAAGATAG ACGACAGGCA GAAGCTGCAG CAGCATTGGC AAAGGAAAAC 
CAAATGCAAC AACTGCAGTT ACAGCAGCAG CAGCAACCGG ACAGTCGTAG AATTTCTGAA 
TCTTCTGAGG GTGAGCCGAA ACAAATTCTT CAAAGGATCA AAGAATCCTC ACCAGCATTG 
GATGAAATTT CAAAAGGAAG AAACGACGAA TTAAAAACAA ATGAGCCTAA ACTGTTATCA 
ACAGCTTGGG AAGAATCAGT AGAAGCCGCT GAAGAAGAAG AACTGAAGGC ATCTGAAGCG 
AGTTTGACTT CAATTGACGA TAAAAAGGAT GTGTCTACTT TGGAGGTAAC TAAAACCGAT 
AGTGGAAAAG TAATCGACCA GGAAAACAAG ATGAGTGTTG ATACTGGAAG GGAAATGGAT 
GACAGCAAAT ATGGTTCAAA GAAACCAATG CACTCAAGAG GAACTGGCAG ACATGAAACA 
CGCGGAGGAG GACATTCTCG AGGAGGCTTT GCTGGTGGTA GTGGATTCCA TCGTGGTGGA 
AGCAGTTCAA GTTGGGGTCG TAGGGGTGGT CCTGGCGGCA ACAGGATGAA CCAGGGTCGT 
AACTACAATC AAGATTACTG GAGTGAGTCA GACTATTCTG ATGATGGCGA CGAAGAATAC 
AGAAAAGATG GCAAATACGA ACGAAAGGAT GGAATGGGTA ATCAATATCG AAATAGCGGT 
TCATCAGTTG GGCAGGCTCA AAATATGAAA GAAGGGTTTG CTCCACGCGG AGAACCATCC 
CGGAGAGGGC GTGGTGGGAT GAGTTCTGCT ATGAGCGGCC CAGGTTTCCG CAAAACTACG 
GTTGGGAAGA AAATGGATGG ATATGGTCCA CCCAGTTCGA AGAGTCCTTT CGGAAGTAGT 
GAAGAAAAAA CAGAATCAAA AAATCAATCT GTGCCGAAAG ATATGAAGCC AGCAGATCAA 
ACATTGAACG CAGACGACCG AACAAAGCAG AAACAAGCTG CTTTGAGTGC TGGGCTTACA 
AACAAACCTG TCGGAGGTCC GAAGCCACCT ACCAGTGGTC CACTGCCTCA AAGAAGCCCT 
TCTGGAAACA TGAAGCCACC TGGCAAGCCA GCTGAAGAAA CCCTTTCTGA TCAAGAAAAA 
TTAAAAGCTT CCTTGGAAAA GAAAACGCGC AATGACAGTA AAACAGAATC TCATGACGAG 
AAGTCGAAAG AGAAACCCAG AACTGAAAGT GAAAGTCATG ATACGGATGA TGCCAGCAAG 
GAGAGCTTGA AAACTGGCCA ACCTCGACCC AAACAACAGC AACCTCCAGG CATTAAGTCA 
GTGCAACCGA GTTCGACGGT ACCCGTGGGA AAACCTTCGA CAACGATTGC AAAGCCAAAT 
ACCGTTGTTC CATCTGGAGC ACCAACAACC GCCGGTCCAA AGCCAACAAC ACCTGCAGCT 
CAACCTGTCA TGCAACAACC TAAGCCGAAT CAAGATCCCA GAGGTCAAAC AAACAATCGA 
TCAATGAACA TTACACCTCG TATGGTCAAA CCAAAGGAAA GACGTGAGGG TGGCTTCCAG 
GATGGACAAC AAAGCGGTGG CGAAACCAAT GAGTCTGAAG CAAACGTTGT GGCTAGTTCC 
CAGCCACAGC CAGTGCAAGA GAAAGTAAAC ACGTTGAACG TCAACTCTTC CAAGACTGAT 
AATAGTAAGC AACATTTAGA TGGAGCCACG CCACCCGTAA ATACGATCAT TTTTGAAAAT 
ACCAACTACA AATCTTCGAC AGTCGTTGTT TCCACAGTGC CACAGCACAT GCTTCAGCCA 
CAACAAATAC AGCAACCACT GCAAATTCAA GTGCAACAGC AACAACCACA AGGTCACGCA 
CCTGCCCAAA TCAAACGGCA ACACTCTGGT GGAGCTGCAC CAGGTCAATT GATTGCACTC 
CATCAACAAC AGCAACAGCA GCAGCAGCAA CAACAACAGC AACAGCAGCT TCAACATCAA 
CAACAAATGG TGCAATTGGT CAAGCAAGAA CATACTGTGC GACATCCATC ACAGGAAGAA 
GCATTCAAGG TCGCAGCTGG TGTCCCACAA TCTGGTTCAA TGCATGACAT TATGGAACAA 
CAACAACAAC AACAGCAACC CCCACAGAGG CCACAGCCAA CAAATGGTCC GCAACAACCA 
CAGCAACAAC AACAATTGCA GCAAAATCAA GTACCATTGC AGCAACAAGT TCCTCAACAG 
CAACAACCTA GTTCTCAAAC CGATGTTATT ACTAGTGCAT TGCATAATAT GTCCTTCCCT 
AAACCTGCGG AAGCCGACTA TGATAAAGAA ATGAAACCAT TCACATTCGA CGCTGATCTT 
TCCCAACTGA TTGAGGCAAA AAATAAAGCA GCTGGGCTCT GTCTTCCTAA GGCACAAAAT 
ATGATTTCGC CATCTACGGC AGAGTTGAAT ATGAAGATTG CTAGTGTGAA AAAAGTATGG 
GAGATGCCTA GTGTCCCGGA ACATGTAACA GAAGATCCGT CAAGTGCGGC AAATGCAGCT 
GCTGCAGCTG TGCAACTGGC AGCCAACTTC GCAGCTGTAA ATTCTCAGCA TCAACAGCAT 
CTTCATCAGT TCCAACACTC ACACAGCAAC TTAACCCATG CTCACCAGGC CCACACTTTG 
GCCGCTGCGG CGGCTGCTGG TCAGTCGTAC GCAGGAGGTT TCGGACAAGA GCAACAATCA 
CTTGATCAAC ACTACAGCAA GGCCAATAAC GATAGCGTAG TGATTGGTGG TACGACAGGA 
GGCAACGATG GTGATAATAA TTATAACAAC CCACAACACG CAGTTGTCGG TCAAGGAAAC 
AATGTTCAGC ATCAATCGGC TGCAAATATG AACATGAAAC ATGATGTTCT AGCCAACAAT 
GTAAATGTAT GCAAGGTGAA ACCAACGCAA CAGAACATGC ATCAATCGAC AGGCTTAGGA 
CTATCGCCTC CACCAATGCA GCAAGGAACT ATTCCATCCG CGCCCCAGAC TTATTTCCAG 
CCATCCCAGT ACGGAATGTC TGCGATTCCT TCGCCACCAG CGGTGATTTA TAATTCGTCA 
GCAATGCCTT CTCAAGGTGG GCTCTACAAT GCATTCCAAA TAGAAGCAGC GGGTCGTTCG 
CAGTTTTCGC AATTCCCCGG TCATTACGGA ACTACCGGAA CTGGTCCATA CAACGCGTAT 
ATGACTACGC CTCCAAATCT GCAAACTGCC CCAACGCCAG ACATGTATCC GAGTATTACT 
TCACAGTTTC GAATGGGCGG TGCCGTCCAA AGTCCGTACA ATCCTTCTCC GTCGCAACAG 
ATGAACAATC CAAGTACAAT GCTGATCTCT TCGAGCACAA ACTCGCTCAT GTCGTCGTCG 
GTAAAACCGT CATCCCAACA AATCGGTGCA ATAGGTTCTA AGAGTGGATC AGTTGGACAA 
CCTTATGGCC AACAATACAT GGGGATGTTC CAACCCGCTC CACCACTGCA GAACAATAGC 
TTCTACTCAA ATTCCGCTGG AGGCCAGAGT GCATTTTTCA GCGGTGCAGG AGCTACACAA 
AATTACGGAA TACCGGCAGC TGCTGCTGGA ATGTTCGGTG GACACGGTGG CCAAACCCCA 
TCGAACGCTC CGCCTCCGCA ACAGCAACTT CCGAACTATC CCAACGCATC GCAGTTCTTA 
AATTCACCGT TAGCGTTGGC GGCAAACGCT ATTTCTCAGC AATTCCGCGG AGGGCCCACA 
ACGAATCCGC AATCTGCCGC TGCAGCCGCA GCATACATGA AAGGAAACCA ACCCCAATCG 
CATATGCAGG ATCCCAATGG TCGGCAGCTG AAAAGCCCTT TGAATTCTGA TGCAATGAAT 
AATTTAAAAC AGATTCCACC CCAAGCGAGT CCGCCACATC ATAAGGGTTA CGCAACAGCA 
TGGGAGATAC AAAATCAACT GATTCAACAA CAACAGCAGC AGCAAAACCA GCAGTCTAAC 
CAACAGCAGC CACCACAACA GCAAAATCCA CAACAACATC CCCAACAGCA AATGAGTCAA 
CCACCGCAGT CTCAGCAGCC CAATCCAAAT CGAAATATGA TGCCCCCCAA CCAGCAACCT 
CCAGCAGGAC TGGGAGGTGC CGGTGGGGGT CGTCCTCCAC AGGGTGTCCA GGGTGGGGGC 
ATGCCTCAGC GATACCCAGC TCCCATCCAA CGACCGACTA ACTACTCCCA GGCGCCAGTA 
CCGGGAATGC ATCAGCAACG GCCCATTCGT CAGTCAGGCC ATAACCCTGG TCAACAACAA 
CAGGCAAACA TGGCACAGGC AATGGGCAAT CCCAACAAAC ATTACTACGG TGGGAATCGA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC5579840 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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