LOC5577522 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5577522
Entrez Gene ID	5577522
Full Name	tubby-related protein 4
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021855529.1	XP_021711221.1	tubby-related protein 4 isoform X1
XM_001656493.1	XP_001656543.1	AAEL003208-PA
XM_021855537.1	XP_021711229.1	tubby-related protein 4 isoform X1
XM_021855552.1	XP_021711244.1	tubby-related protein 4 isoform X2
XM_021855559.1	XP_021711251.1	tubby-related protein 4 isoform X3
XM_021855522.1	XP_021711214.1	tubby-related protein 4 isoform X1
XM_021855544.1	XP_021711236.1	tubby-related protein 4 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5577522 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5577522 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe12278	XM_001656493.1 Latest version!	Aedes aegypti AAEL003208-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAe115723	XM_021855537.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe115723	XM_021855529.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe115723	XM_021855522.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe115723	XM_021855544.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe115724	XM_021855552.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe115725	XM_021855559.1 Latest version!	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5577522 ( XM_001656493.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001656543.1 Aedes aegypti AAEL003208-RA partial mRNA.

Price & Availability↑

CloneID	OAe12278
Clone ID Related Accession (Same CDS sequence)	XM_001656493.1
Accession Version	XM_001656493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL003208-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811585). COMPLETENESS: incomplete on both ends.

ATGGTGGCGG GGTTGTACAG GGAGGAAGGA TGGAAGTTGA ACCGTACGAA CTACTATCAG 
GAAGGATGGC TAGCAACTGG CAACGTGCGA GGCATCGTTG GAGTTACCTT CACAACGTCA 
CACTGCAAGA AGAACGTCGA ATATCCCCTG AGGACAAACT ACAACCTGCG AGGGCACCGC 
TCAGACGTGA TTCTGGTGAA ATGGAACGAG CCGTACCAGA AGTTGGCAAG CTGTGATAGC 
TCCGGTATCA TCTTCGTGTG GATAAAATAC GAAGGACGAT GGAGCGTGGA GCTAATAAAC 
GACAGAAACA CGCCGGTAAC ACATTTTTCC TGGTCACACG ACGGAAGGAT GGCACTAATA 
TGCTATCAGG ATGGTTTCGT GTTGGTTGGC TCCGTCGCTG GACAACGGTA CTGGTCATCA 
ATGCTGAACC TGGATGCTAC TATCACGTGC GGAATCTGGA CGCCAGACGA TCAGCAGGTG 
TATTTTGGCA CAACGCAGGG CCAGATAATT GTGATGGACG TGCACGGGGC GATGGTTTCA 
CAGGTTCAGC TTGGTTCGGA TACCGGAATC ACTGCAATGG CATGGTCATG CGAAAAGTTC 
AAAATGGAGG AAGGTGAAGA CACGGAACCA GGAGTTACAA ATGCTTCCAA ACGCTCTTTT 
GTGTTAGCAG TAAGCTTCCA GAATGGATAC ATTTACTTAC TGAAGTCTTT CGATGATGTT 
TCGCCTTGTC ACATACATAC GGAAATGAAC GGAAATCTAG GCTTTGTGAT GGAGTGGAGT 
AATTCGCGTG AGTTATTGGC TGTGGCTGGT AGTGAAATAG CCGCTGAATC GGAAACGACT 
GAAACACCTG CAATGCCAGT GTACAATAAT CTTCTGAAGT TTTACACAGA ATCAGGAAAT 
CTACTATACA CAGCTAAAAT ACCTTGCACT ACGACTCCAG TATCTGCCCT AACGTGGGGT 
CACAACGACA AACGAATATT TATTGCTACG GGAACACAGG TTCACATCGC ATGGGTGTCG 
CGAAGGGTTG CATCTTTGCA ATTGTTTTGT CGACTACAAG TACAATCTTC GCTAACTTCA 
GAAGCCATGC TGCCCAAATT ACCACTACCA GGTCGAATCA AAGCACTGAT TGGCAATCTT 
TTCGCCCAAA CCATTCGGTG CTGTGTTCCG GATCTGAAAT ATTTACGAGA ATTTGTGTCT 
CGACCACCAG TGTGTTCCAC CCGGTTACAT TGTACGATGA TCCGACATGA TGAAGACGCA 
AATCAAAGTT CTGGTACTTG TTACACATTG TACCTGGAAT TTCTTGGAGG TTTAGTTCCG 
CTTCTCAAAG GCAAAAGGAC GTCGAAAATC CGGCCGGAAT TTGTGATATT TGATCCACAG 
ATCGATGAAA TATCCTCTTA CGTCACCTAC TCATCAGATT CAACGATCAC AACTTCAAAA 
AGTTCATCAG CGTCCAGTCA TTCGACGAGT GGGACCACCG GAAGATCAGA CAGTTCGGAA 
AGTGATATAG AAGAAGGTTG TCGTTCGCCT CGATTGTCCA GAAAACGAAA AGCACGAACT 
AAAAAGCGAC AACCGAGTAA TAGCGATCGC TCTCCTAATC AAAACTCTGA TTCTACTGAG 
AATAACGAGG AATTGGCATA CCTGGATACA TTACCAGAGC ATGTCAAACT GGTGGAAGTC 
ACGTCAAATA TCTGGGGAAC TAAGTTTAAA ATTCACGGTT TAGCAAAAAC ACTTCCGGCA 
AATCTTGGTC AAGTTACTTA TAAAACGTCT TTACTTCACC TACAACCACG CCAAATGACC 
TTAGTTATTA CTGAACTTCG GGATGATTTC CCAACTGGGC CGGACCCGAA CTTTAATCCC 
AACATATTTT CAGAAGATGA AGAAGAACAC AATCAAACTC ATTTTGGTTC TGGTGGTCGC 
AAACTTGTAG AAACCGCTCC TCCGATAGCC CCTATGTCAC CTCGTCCAAA TCGATTTCCT 
TCTCGACCGC GTCGTCAAAC ACCTTCTTCG CTGGCATCTG CCTCCAACGG TCGTCCTCCA 
TTGCCTCATC TTGGCCCTCT AGCTCGTGCC GAATCCTATG AAGATGACAC CGATGGACCA 
GATGGAGAAC CGTCACGCAC TGTTGCAAAT CCTCCCCGTC AAGGAATTAG TTATTCTAGT 
TTAGTTAGCC AATGCAGTAG ACCATCATCT GCGTCTAGTA GTCAATCACG TGTTGCCATA 
TCTCCACTGT ACTGCGAAGG TTCCGTTCCA ACGCTACAGT CACCGAAGAA TGCTGTAGCT 
CCTAGTGATA TTATTTTTGA TAGACCACCA GCCGGACAAA CTACCCTTAT GAGTTATTCA 
AGTAATACGG ATTACGTTAA CAATGTTGTT CAGGTGAAAA ACGCCATTAT GTCCTCGGAT 
CATAGTCGTA CCAACAATCA GTCTCACGTC AACCCGGTAC CATTAAATCT AAATCTCAAT 
TTGGAACGGA TAGAAACCAA AGCTAACAAT AGTAGTAGTA GAGACTCGTC ACATTCCACG 
GGTAGTGGTA GTAAGCATGG AACTCCGAAG AAGCAAAATT TAAAATACAT CGACGAAGAA 
ACTCCATCTA CCTCTGCTAC GCCAGTTGTT CCAGAGCCTA GCACCAGTGG AACTAGCAAC 
TGCAGCAGTA ACCCATCTGG TGCTATCATG AAACGCACTC CGACCGTAAT TTCAATGACT 
CCTGTTGTGG CGAGTGGTAT TCCAGAGTCT ATCACACGTA GCTGTAGTGT AGGCTATCTA 
GATAATATGG AAATTGTCCC TAGTGATGCT GTACTTACGA TGATGCGTAA AGAAACTCCT 
TATAAACGGT TAGTTTTGGT TGATAAGAAG CGGCAGAAAA AGTACCGTCG TAATTTTGAT 
GATATGAAGA AAGCAAAACT GCGCCAAGGT TGCAAATCTA AAAGTTTAGA CTCGTGCGAC 
ATACTGCAGG ATTCGCCCAA TTTGAACCGA GACCTGATAA ATCTTTTCCG GCAGATGCCC 
AAAGTGAACG AGCTTTCAGA GAATGAAACT GAATACTCTT CGTCGGATCA GACCGATCGA 
ATGCGTCCGA ATGCGCCAAC TGGATCATCC TCCGCAGCAT CAACAAAACG AAGACCATCA 
ATGAAATTTC CAGGAGTAAC AGGCCCTATT CTACCTATTT CCAAAACACC TATTCTCAAT 
CGGAAGGAGA AGCCAAAATC TTGTTCTATA TTTACAAGCT TTACCGACAG TCCTCTATTT 
AGTCGAAGAC ATCGTTCCTT GAATAGAACC GACTCGTCAA AATTGCAGAG TGAAAGCAGC 
ACTCCAGTAA TGGCTCGCAA GTCCGAAACA AGTAAAAGTG AAAACGGCGA AAGTCGAAAA 
AGCAGACGAA AAGACGAATC ACCTCGGCGA CATCGTCGCA ACGATAGTTG TCCTGGTCCA 
AGTATAGAAA ACAACAATGG CGAGGGACCA TCCCTTCAAC CAGTGGAAAT TACTCCCGTT 
GAGCAGAAAG CTTCGGTTTC GCTTCACACG CAAGCTTTGA CCACCCTTGA AAACATCATC 
AGCCGGCTGA GGGATCTTGA CGAAGGCCGT CTAACGCCAC CCTCATCTCC GTCGAATTCT 
CGTTTGCCAC GCAGTTCACC GGCTTCACCT GCTCCGAGTA AAAAGAGCAA ACGTCACCAA 
AGTGCTTCGC CTATTCGTCA TATATTGAAT TCACCGCTAC TGAATCGGAG GCAGCGGAAG 
AAGCAGCATA CGGAAAGCTC TGACGATGAA AATCAATTGT TGACCCAGAC AGACGACAGT 
AGCGGAGGAA GTGGGAGTAA CAACAACAAG CAGTATCGTG ACTTGGAAAC GTTTCAAAAG 
GCTCAACTCC GACAAAAGCT AAAACGTGGT AAAATTGAGC CAAATGGAAT TACGAGTTCC 
TTTCAGCCGC AGGCTCCAGT GAGACGAGAA TTCGTTATGC ACAATAAGGC TCCCATGTGG 
AATGAAAACA GCCAAGTGTA TCAACTGGAC TTCGGTGGAC GAGTGACCCA GGAGTCGGCT 
AAGAATTTCC AGATAGAATT CCGTGGCAAA CAGGTCATGC AGTTCGGTCG GATTGACGGT 
AATGCGTACA CGTTGGATTT CCAATATCCC TTTTCGGCAC TGCAAGCTTT CGCCGTCGCT 
CTAGCGAACG TTACTCAGCG GTTAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001656543.1
CDS	1..4170
Translation	MVAGLYREEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIETKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSIFTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_001656493.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL003208-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001656493.1

ORF Insert Sequence:

ATGGTGGCGG GGTTGTACAG GGAGGAAGGA TGGAAGTTGA ACCGTACGAA CTACTATCAG 
GAAGGATGGC TAGCAACTGG CAACGTGCGA GGCATCGTTG GAGTTACCTT CACAACGTCA 
CACTGCAAGA AGAACGTCGA ATATCCCCTG AGGACAAACT ACAACCTGCG AGGGCACCGC 
TCAGACGTGA TTCTGGTGAA ATGGAACGAG CCGTACCAGA AGTTGGCAAG CTGTGATAGC 
TCCGGTATCA TCTTCGTGTG GATAAAATAC GAAGGACGAT GGAGCGTGGA GCTAATAAAC 
GACAGAAACA CGCCGGTAAC ACATTTTTCC TGGTCACACG ACGGAAGGAT GGCACTAATA 
TGCTATCAGG ATGGTTTCGT GTTGGTTGGC TCCGTCGCTG GACAACGGTA CTGGTCATCA 
ATGCTGAACC TGGATGCTAC TATCACGTGC GGAATCTGGA CGCCAGACGA TCAGCAGGTG 
TATTTTGGCA CAACGCAGGG CCAGATAATT GTGATGGACG TGCACGGGGC GATGGTTTCA 
CAGGTTCAGC TTGGTTCGGA TACCGGAATC ACTGCAATGG CATGGTCATG CGAAAAGTTC 
AAAATGGAGG AAGGTGAAGA CACGGAACCA GGAGTTACAA ATGCTTCCAA ACGCTCTTTT 
GTGTTAGCAG TAAGCTTCCA GAATGGATAC ATTTACTTAC TGAAGTCTTT CGATGATGTT 
TCGCCTTGTC ACATACATAC GGAAATGAAC GGAAATCTAG GCTTTGTGAT GGAGTGGAGT 
AATTCGCGTG AGTTATTGGC TGTGGCTGGT AGTGAAATAG CCGCTGAATC GGAAACGACT 
GAAACACCTG CAATGCCAGT GTACAATAAT CTTCTGAAGT TTTACACAGA ATCAGGAAAT 
CTACTATACA CAGCTAAAAT ACCTTGCACT ACGACTCCAG TATCTGCCCT AACGTGGGGT 
CACAACGACA AACGAATATT TATTGCTACG GGAACACAGG TTCACATCGC ATGGGTGTCG 
CGAAGGGTTG CATCTTTGCA ATTGTTTTGT CGACTACAAG TACAATCTTC GCTAACTTCA 
GAAGCCATGC TGCCCAAATT ACCACTACCA GGTCGAATCA AAGCACTGAT TGGCAATCTT 
TTCGCCCAAA CCATTCGGTG CTGTGTTCCG GATCTGAAAT ATTTACGAGA ATTTGTGTCT 
CGACCACCAG TGTGTTCCAC CCGGTTACAT TGTACGATGA TCCGACATGA TGAAGACGCA 
AATCAAAGTT CTGGTACTTG TTACACATTG TACCTGGAAT TTCTTGGAGG TTTAGTTCCG 
CTTCTCAAAG GCAAAAGGAC GTCGAAAATC CGGCCGGAAT TTGTGATATT TGATCCACAG 
ATCGATGAAA TATCCTCTTA CGTCACCTAC TCATCAGATT CAACGATCAC AACTTCAAAA 
AGTTCATCAG CGTCCAGTCA TTCGACGAGT GGGACCACCG GAAGATCAGA CAGTTCGGAA 
AGTGATATAG AAGAAGGTTG TCGTTCGCCT CGATTGTCCA GAAAACGAAA AGCACGAACT 
AAAAAGCGAC AACCGAGTAA TAGCGATCGC TCTCCTAATC AAAACTCTGA TTCTACTGAG 
AATAACGAGG AATTGGCATA CCTGGATACA TTACCAGAGC ATGTCAAACT GGTGGAAGTC 
ACGTCAAATA TCTGGGGAAC TAAGTTTAAA ATTCACGGTT TAGCAAAAAC ACTTCCGGCA 
AATCTTGGTC AAGTTACTTA TAAAACGTCT TTACTTCACC TACAACCACG CCAAATGACC 
TTAGTTATTA CTGAACTTCG GGATGATTTC CCAACTGGGC CGGACCCGAA CTTTAATCCC 
AACATATTTT CAGAAGATGA AGAAGAACAC AATCAAACTC ATTTTGGTTC TGGTGGTCGC 
AAACTTGTAG AAACCGCTCC TCCGATAGCC CCTATGTCAC CTCGTCCAAA TCGATTTCCT 
TCTCGACCGC GTCGTCAAAC ACCTTCTTCG CTGGCATCTG CCTCCAACGG TCGTCCTCCA 
TTGCCTCATC TTGGCCCTCT AGCTCGTGCC GAATCCTATG AAGATGACAC CGATGGACCA 
GATGGAGAAC CGTCACGCAC TGTTGCAAAT CCTCCCCGTC AAGGAATTAG TTATTCTAGT 
TTAGTTAGCC AATGCAGTAG ACCATCATCT GCGTCTAGTA GTCAATCACG TGTTGCCATA 
TCTCCACTGT ACTGCGAAGG TTCCGTTCCA ACGCTACAGT CACCGAAGAA TGCTGTAGCT 
CCTAGTGATA TTATTTTTGA TAGACCACCA GCCGGACAAA CTACCCTTAT GAGTTATTCA 
AGTAATACGG ATTACGTTAA CAATGTTGTT CAGGTGAAAA ACGCCATTAT GTCCTCGGAT 
CATAGTCGTA CCAACAATCA GTCTCACGTC AACCCGGTAC CATTAAATCT AAATCTCAAT 
TTGGAACGGA TAGAAACCAA AGCTAACAAT AGTAGTAGTA GAGACTCGTC ACATTCCACG 
GGTAGTGGTA GTAAGCATGG AACTCCGAAG AAGCAAAATT TAAAATACAT CGACGAAGAA 
ACTCCATCTA CCTCTGCTAC GCCAGTTGTT CCAGAGCCTA GCACCAGTGG AACTAGCAAC 
TGCAGCAGTA ACCCATCTGG TGCTATCATG AAACGCACTC CGACCGTAAT TTCAATGACT 
CCTGTTGTGG CGAGTGGTAT TCCAGAGTCT ATCACACGTA GCTGTAGTGT AGGCTATCTA 
GATAATATGG AAATTGTCCC TAGTGATGCT GTACTTACGA TGATGCGTAA AGAAACTCCT 
TATAAACGGT TAGTTTTGGT TGATAAGAAG CGGCAGAAAA AGTACCGTCG TAATTTTGAT 
GATATGAAGA AAGCAAAACT GCGCCAAGGT TGCAAATCTA AAAGTTTAGA CTCGTGCGAC 
ATACTGCAGG ATTCGCCCAA TTTGAACCGA GACCTGATAA ATCTTTTCCG GCAGATGCCC 
AAAGTGAACG AGCTTTCAGA GAATGAAACT GAATACTCTT CGTCGGATCA GACCGATCGA 
ATGCGTCCGA ATGCGCCAAC TGGATCATCC TCCGCAGCAT CAACAAAACG AAGACCATCA 
ATGAAATTTC CAGGAGTAAC AGGCCCTATT CTACCTATTT CCAAAACACC TATTCTCAAT 
CGGAAGGAGA AGCCAAAATC TTGTTCTATA TTTACAAGCT TTACCGACAG TCCTCTATTT 
AGTCGAAGAC ATCGTTCCTT GAATAGAACC GACTCGTCAA AATTGCAGAG TGAAAGCAGC 
ACTCCAGTAA TGGCTCGCAA GTCCGAAACA AGTAAAAGTG AAAACGGCGA AAGTCGAAAA 
AGCAGACGAA AAGACGAATC ACCTCGGCGA CATCGTCGCA ACGATAGTTG TCCTGGTCCA 
AGTATAGAAA ACAACAATGG CGAGGGACCA TCCCTTCAAC CAGTGGAAAT TACTCCCGTT 
GAGCAGAAAG CTTCGGTTTC GCTTCACACG CAAGCTTTGA CCACCCTTGA AAACATCATC 
AGCCGGCTGA GGGATCTTGA CGAAGGCCGT CTAACGCCAC CCTCATCTCC GTCGAATTCT 
CGTTTGCCAC GCAGTTCACC GGCTTCACCT GCTCCGAGTA AAAAGAGCAA ACGTCACCAA 
AGTGCTTCGC CTATTCGTCA TATATTGAAT TCACCGCTAC TGAATCGGAG GCAGCGGAAG 
AAGCAGCATA CGGAAAGCTC TGACGATGAA AATCAATTGT TGACCCAGAC AGACGACAGT 
AGCGGAGGAA GTGGGAGTAA CAACAACAAG CAGTATCGTG ACTTGGAAAC GTTTCAAAAG 
GCTCAACTCC GACAAAAGCT AAAACGTGGT AAAATTGAGC CAAATGGAAT TACGAGTTCC 
TTTCAGCCGC AGGCTCCAGT GAGACGAGAA TTCGTTATGC ACAATAAGGC TCCCATGTGG 
AATGAAAACA GCCAAGTGTA TCAACTGGAC TTCGGTGGAC GAGTGACCCA GGAGTCGGCT 
AAGAATTTCC AGATAGAATT CCGTGGCAAA CAGGTCATGC AGTTCGGTCG GATTGACGGT 
AATGCGTACA CGTTGGATTT CCAATATCCC TTTTCGGCAC TGCAAGCTTT CGCCGTCGCT 
CTAGCGAACG TTACTCAGCG GTTAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855529.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711221.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAe115723
Clone ID Related Accession (Same CDS sequence)	XM_021855529.1 , XM_021855537.1 , XM_021855522.1 , XM_021855544.1
Accession Version	XM_021855529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711221.1
CDS	704..5311
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855529.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855529.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855537.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711229.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X3, mRNA.

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CloneID	OAe115723
Clone ID Related Accession (Same CDS sequence)	XM_021855529.1 , XM_021855537.1 , XM_021855522.1 , XM_021855544.1
Accession Version	XM_021855537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711229.1
CDS	1040..5647
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855537.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855537.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855522.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711214.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X1, mRNA.

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CloneID	OAe115723
Clone ID Related Accession (Same CDS sequence)	XM_021855529.1 , XM_021855537.1 , XM_021855522.1 , XM_021855544.1
Accession Version	XM_021855522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711214.1
CDS	1143..5750
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855522.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855522.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855544.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711236.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X4, mRNA.

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CloneID	OAe115723
Clone ID Related Accession (Same CDS sequence)	XM_021855529.1 , XM_021855537.1 , XM_021855522.1 , XM_021855544.1
Accession Version	XM_021855544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711236.1
CDS	364..4971
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855544.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855544.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTGTCGTT CGCCTCGATT GTCCAGAAAA 
CGAAAAGCAC GAACTAAAAA GCGACAACCG AGTAATAGCG ATCGCTCTCC TAATCAAAAC 
TCTGATTCTA CTGAGAATAA CGAGGAATTG GCATACCTGG ATACATTACC AGAGCATGTC 
AAACTGGTGG AAGTCACGTC AAATATCTGG GGAACTAAGT TTAAAATTCA CGGTTTAGCA 
AAAACACTTC CGGCAAATCT TGGTCAAGTT ACTTATAAAA CGTCTTTACT TCACCTACAA 
CCACGCCAAA TGACCTTAGT TATTACTGAA CTTCGGGATG ATTTCCCAAC TGGGCCGGAC 
CCGAACTTTA ATCCCAACAT ATTTTCAGAA GATGAAGAAG AACACAATCA AACTCATTTT 
GGTTCTGGTG GTCGCAAACT TGTAGAAACC GCTCCTCCGA TAGCCCCTAT GTCACCTCGT 
CCAAATCGAT TTCCTTCTCG ACCGCGTCGT CAAACACCTT CTTCGCTGGC ATCTGCCTCC 
AACGGTCGTC CTCCATTGCC TCATCTTGGC CCTCTAGCTC GTGCCGAATC CTATGAAGAT 
GACACCGATG GACCAGATGG AGAACCGTCA CGCACTGTTG CAAATCCTCC CCGTCAAGGA 
ATTAGTTATT CTAGTTTAGT TAGCCAATGC AGTAGACCAT CATCTGCGTC TAGTAGTCAA 
TCACGTGTTG CCATATCTCC ACTGTACTGC GAAGGTTCCG TTCCAACGCT ACAGTCACCG 
AAGAATGCTG TAGCTCCTAG TGATATTATT TTTGATAGAC CACCAGCCGG ACAAACTACC 
CTTATGAGTT ATTCAAGTAA TACGGATTAC GTTAACAATG TTGTTCAGGT GAAAAACGCC 
ATTATGTCCT CGGATCATAG TCGTACCAAC AATCAGTCTC ACGTCAACCC GGTACCATTA 
AATCTAAATC TCAATTTGGA ACGGATAGAA GCCAAAGCTA ACAATAGTAG TAGTAGAGAC 
TCGTCACATT CCACGGGTAG TGGTAGTAAG CATGGAACTC CGAAGAAGCA AAATTTAAAA 
TACATCGACG AAGAAACTCC ATCTACCTCT GCTACGCCAG TTGTTCCAGA GCCTAGCACC 
AGTGGAACTA GCAACTGCAG CAGTAACCCA TCTGGTGCTA TCATGAAACG CACTCCGACC 
GTAATTTCAA TGACTCCTGT TGTGGCGAGT GGTATTCCAG AGTCTATCAC ACGTAGCTGT 
AGTGTAGGCT ATCTAGATAA TATGGAAATT GTCCCTAGTG ATGCTGTACT TACGATGATG 
CGTAAAGAAA CTCCTTATAA ACGGTTAGTT TTGGTTGATA AGAAGCGGCA GAAAAAGTAC 
CGTCGTAATT TTGATGATAT GAAGAAAGCA AAACTGCGCC AAGGTTGCAA ATCTAAAAGT 
TTAGACTCGT GCGACATACT GCAGGATTCG CCCAATTTGA ACCGAGACCT GATAAATCTT 
TTCCGGCAGA TGCCCAAAGT GAACGAGCTT TCAGAGAATG AAACTGAATA CTCTTCGTCG 
GATCAGACCG ATCGAATGCG TCCGAATGCG CCAACTGGAT CATCCTCCGC AGCATCAACA 
AAACGAAGAC CATCAATGAA ATTTCCAGGA GTAACAGGCC CTATTCTACC TATTTCCAAA 
ACACCTATTC TCAATCGGAA GGAGAAGCCA AAATCTTGTT CTATATGTAA AACGATTTCT 
CCGACTGTTA ACTCCACAGA AACAGTATGT GTGAAGTGCC GAAAGAATGG AGGAACTTCC 
AATGGAGTAG ACAAAGAAAA TAATAGTATA GAAGACCACC AAAACCACAT TTACGTAGAT 
ATAATAAGCG CGAAACCTCC ACAAGCTCCC CCCAAAGCCA AGCGATCAAT ACTTGAGCAA 
TACATTAGTT CAGTTTATAG TAATAACGGA GAATTGGTAC TTAAGGATAC CTCTAGTGGA 
ACCAGTGGAA CGAAAACTAC TAACGACAAG AAAAACAACA ATAGTAATGG AGATACTAAT 
GGATCGTGTA GCACGCCGAA GTCCAAAATT AGGAAAAATT TCGAGATAGT TACAAGCTTT 
ACCGACAGTC CTCTATTTAG TCGAAGACAT CGTTCCTTGA ATAGAACCGA CTCGTCAAAA 
TTGCAGAGTG AAAGCAGCAC TCCAGTAATG GCTCGCAAGT CCGAAACAAG TAAAAGTGAA 
AACGGCGAAA GTCGAAAAAG CAGACGAAAA GACGAATCAC CTCGGCGACA TCGTCGCAAC 
GATAGTTGTC CTGGTCCAAG TATAGAAAAC AACAATGGCG AGGGACCATC CCTTCAACCA 
GTGGAAATTA CTCCCGTTGA GCAGAAAGCT TCGGTTTCGC TTCACACGCA AGCTTTGACC 
ACCCTTGAAA ACATCATCAG CCGGCTGAGG GATCTTGACG AAGGCCGTCT AACGCCACCC 
TCATCTCCGT CGAATTCTCG TTTGCCACGC AGTTCACCGG CTTCACCTGC TCCGAGTAAA 
AAGAGCAAAC GTCACCAAAG TGCTTCGCCT ATTCGTCATA TATTGAATTC ACCGCTACTG 
AATCGGAGGC AGCGGAAGAA GCAGCATACG GAAAGCTCTG ACGATGAAAA TCAATTGTTG 
ACCCAGACAG ACGACAGTAG CGGAGGAAGT GGGAGTAACA ACAACAAGCA GTATCGTGAC 
TTGGAAACGT TTCAAAAGGC TCAACTCCGA CAAAAGCTAA AACGTGGTAA AATTGAGCCA 
AATGGAATTA CGAGTTCCTT TCAGCCGCAG GCTCCAGTGA GACGAGAATT CGTTATGCAC 
AATAAGGCTC CCATGTGGAA TGAAAACAGC CAAGTGTATC AACTGGACTT CGGTGGACGA 
GTGACCCAGG AGTCGGCTAA GAATTTCCAG ATAGAATTCC GTGGCAAACA GGTCATGCAG 
TTCGGTCGGA TTGACGGTAA TGCGTACACG TTGGATTTCC AATATCCCTT TTCGGCACTG 
CAAGCTTTCG CCGTCGCTCT AGCGAACGTT ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855552.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711244.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAe115724
Clone ID Related Accession (Same CDS sequence)	XM_021855552.1
Accession Version	XM_021855552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTCGCCTC GATTGTCCAG AAAACGAAAA 
GCACGAACTA AAAAGCGACA ACCGAGTAAT AGCGATCGCT CTCCTAATCA AAACTCTGAT 
TCTACTGAGA ATAACGAGGA ATTGGCATAC CTGGATACAT TACCAGAGCA TGTCAAACTG 
GTGGAAGTCA CGTCAAATAT CTGGGGAACT AAGTTTAAAA TTCACGGTTT AGCAAAAACA 
CTTCCGGCAA ATCTTGGTCA AGTTACTTAT AAAACGTCTT TACTTCACCT ACAACCACGC 
CAAATGACCT TAGTTATTAC TGAACTTCGG GATGATTTCC CAACTGGGCC GGACCCGAAC 
TTTAATCCCA ACATATTTTC AGAAGATGAA GAAGAACACA ATCAAACTCA TTTTGGTTCT 
GGTGGTCGCA AACTTGTAGA AACCGCTCCT CCGATAGCCC CTATGTCACC TCGTCCAAAT 
CGATTTCCTT CTCGACCGCG TCGTCAAACA CCTTCTTCGC TGGCATCTGC CTCCAACGGT 
CGTCCTCCAT TGCCTCATCT TGGCCCTCTA GCTCGTGCCG AATCCTATGA AGATGACACC 
GATGGACCAG ATGGAGAACC GTCACGCACT GTTGCAAATC CTCCCCGTCA AGGAATTAGT 
TATTCTAGTT TAGTTAGCCA ATGCAGTAGA CCATCATCTG CGTCTAGTAG TCAATCACGT 
GTTGCCATAT CTCCACTGTA CTGCGAAGGT TCCGTTCCAA CGCTACAGTC ACCGAAGAAT 
GCTGTAGCTC CTAGTGATAT TATTTTTGAT AGACCACCAG CCGGACAAAC TACCCTTATG 
AGTTATTCAA GTAATACGGA TTACGTTAAC AATGTTGTTC AGGTGAAAAA CGCCATTATG 
TCCTCGGATC ATAGTCGTAC CAACAATCAG TCTCACGTCA ACCCGGTACC ATTAAATCTA 
AATCTCAATT TGGAACGGAT AGAAGCCAAA GCTAACAATA GTAGTAGTAG AGACTCGTCA 
CATTCCACGG GTAGTGGTAG TAAGCATGGA ACTCCGAAGA AGCAAAATTT AAAATACATC 
GACGAAGAAA CTCCATCTAC CTCTGCTACG CCAGTTGTTC CAGAGCCTAG CACCAGTGGA 
ACTAGCAACT GCAGCAGTAA CCCATCTGGT GCTATCATGA AACGCACTCC GACCGTAATT 
TCAATGACTC CTGTTGTGGC GAGTGGTATT CCAGAGTCTA TCACACGTAG CTGTAGTGTA 
GGCTATCTAG ATAATATGGA AATTGTCCCT AGTGATGCTG TACTTACGAT GATGCGTAAA 
GAAACTCCTT ATAAACGGTT AGTTTTGGTT GATAAGAAGC GGCAGAAAAA GTACCGTCGT 
AATTTTGATG ATATGAAGAA AGCAAAACTG CGCCAAGGTT GCAAATCTAA AAGTTTAGAC 
TCGTGCGACA TACTGCAGGA TTCGCCCAAT TTGAACCGAG ACCTGATAAA TCTTTTCCGG 
CAGATGCCCA AAGTGAACGA GCTTTCAGAG AATGAAACTG AATACTCTTC GTCGGATCAG 
ACCGATCGAA TGCGTCCGAA TGCGCCAACT GGATCATCCT CCGCAGCATC AACAAAACGA 
AGACCATCAA TGAAATTTCC AGGAGTAACA GGCCCTATTC TACCTATTTC CAAAACACCT 
ATTCTCAATC GGAAGGAGAA GCCAAAATCT TGTTCTATAT GTAAAACGAT TTCTCCGACT 
GTTAACTCCA CAGAAACAGT ATGTGTGAAG TGCCGAAAGA ATGGAGGAAC TTCCAATGGA 
GTAGACAAAG AAAATAATAG TATAGAAGAC CACCAAAACC ACATTTACGT AGATATAATA 
AGCGCGAAAC CTCCACAAGC TCCCCCCAAA GCCAAGCGAT CAATACTTGA GCAATACATT 
AGTTCAGTTT ATAGTAATAA CGGAGAATTG GTACTTAAGG ATACCTCTAG TGGAACCAGT 
GGAACGAAAA CTACTAACGA CAAGAAAAAC AACAATAGTA ATGGAGATAC TAATGGATCG 
TGTAGCACGC CGAAGTCCAA AATTAGGAAA AATTTCGAGA TAGTTACAAG CTTTACCGAC 
AGTCCTCTAT TTAGTCGAAG ACATCGTTCC TTGAATAGAA CCGACTCGTC AAAATTGCAG 
AGTGAAAGCA GCACTCCAGT AATGGCTCGC AAGTCCGAAA CAAGTAAAAG TGAAAACGGC 
GAAAGTCGAA AAAGCAGACG AAAAGACGAA TCACCTCGGC GACATCGTCG CAACGATAGT 
TGTCCTGGTC CAAGTATAGA AAACAACAAT GGCGAGGGAC CATCCCTTCA ACCAGTGGAA 
ATTACTCCCG TTGAGCAGAA AGCTTCGGTT TCGCTTCACA CGCAAGCTTT GACCACCCTT 
GAAAACATCA TCAGCCGGCT GAGGGATCTT GACGAAGGCC GTCTAACGCC ACCCTCATCT 
CCGTCGAATT CTCGTTTGCC ACGCAGTTCA CCGGCTTCAC CTGCTCCGAG TAAAAAGAGC 
AAACGTCACC AAAGTGCTTC GCCTATTCGT CATATATTGA ATTCACCGCT ACTGAATCGG 
AGGCAGCGGA AGAAGCAGCA TACGGAAAGC TCTGACGATG AAAATCAATT GTTGACCCAG 
ACAGACGACA GTAGCGGAGG AAGTGGGAGT AACAACAACA AGCAGTATCG TGACTTGGAA 
ACGTTTCAAA AGGCTCAACT CCGACAAAAG CTAAAACGTG GTAAAATTGA GCCAAATGGA 
ATTACGAGTT CCTTTCAGCC GCAGGCTCCA GTGAGACGAG AATTCGTTAT GCACAATAAG 
GCTCCCATGT GGAATGAAAA CAGCCAAGTG TATCAACTGG ACTTCGGTGG ACGAGTGACC 
CAGGAGTCGG CTAAGAATTT CCAGATAGAA TTCCGTGGCA AACAGGTCAT GCAGTTCGGT 
CGGATTGACG GTAATGCGTA CACGTTGGAT TTCCAATATC CCTTTTCGGC ACTGCAAGCT 
TTCGCCGTCG CTCTAGCGAA CGTTACTCAG CGGTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711244.1
CDS	1409..6010
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDEISSYVTYSSDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855552.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855552.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGAAATATCC TCTTACGTCA CCTACTCATC AGATTCAACG 
ATCACAACTT CAAAAAGTTC ATCAGCGTCC AGTCATTCGA CGAGTGGGAC CACCGGAAGA 
TCAGACAGTT CGGAAAGTGA TATAGAAGAA GGTTCGCCTC GATTGTCCAG AAAACGAAAA 
GCACGAACTA AAAAGCGACA ACCGAGTAAT AGCGATCGCT CTCCTAATCA AAACTCTGAT 
TCTACTGAGA ATAACGAGGA ATTGGCATAC CTGGATACAT TACCAGAGCA TGTCAAACTG 
GTGGAAGTCA CGTCAAATAT CTGGGGAACT AAGTTTAAAA TTCACGGTTT AGCAAAAACA 
CTTCCGGCAA ATCTTGGTCA AGTTACTTAT AAAACGTCTT TACTTCACCT ACAACCACGC 
CAAATGACCT TAGTTATTAC TGAACTTCGG GATGATTTCC CAACTGGGCC GGACCCGAAC 
TTTAATCCCA ACATATTTTC AGAAGATGAA GAAGAACACA ATCAAACTCA TTTTGGTTCT 
GGTGGTCGCA AACTTGTAGA AACCGCTCCT CCGATAGCCC CTATGTCACC TCGTCCAAAT 
CGATTTCCTT CTCGACCGCG TCGTCAAACA CCTTCTTCGC TGGCATCTGC CTCCAACGGT 
CGTCCTCCAT TGCCTCATCT TGGCCCTCTA GCTCGTGCCG AATCCTATGA AGATGACACC 
GATGGACCAG ATGGAGAACC GTCACGCACT GTTGCAAATC CTCCCCGTCA AGGAATTAGT 
TATTCTAGTT TAGTTAGCCA ATGCAGTAGA CCATCATCTG CGTCTAGTAG TCAATCACGT 
GTTGCCATAT CTCCACTGTA CTGCGAAGGT TCCGTTCCAA CGCTACAGTC ACCGAAGAAT 
GCTGTAGCTC CTAGTGATAT TATTTTTGAT AGACCACCAG CCGGACAAAC TACCCTTATG 
AGTTATTCAA GTAATACGGA TTACGTTAAC AATGTTGTTC AGGTGAAAAA CGCCATTATG 
TCCTCGGATC ATAGTCGTAC CAACAATCAG TCTCACGTCA ACCCGGTACC ATTAAATCTA 
AATCTCAATT TGGAACGGAT AGAAGCCAAA GCTAACAATA GTAGTAGTAG AGACTCGTCA 
CATTCCACGG GTAGTGGTAG TAAGCATGGA ACTCCGAAGA AGCAAAATTT AAAATACATC 
GACGAAGAAA CTCCATCTAC CTCTGCTACG CCAGTTGTTC CAGAGCCTAG CACCAGTGGA 
ACTAGCAACT GCAGCAGTAA CCCATCTGGT GCTATCATGA AACGCACTCC GACCGTAATT 
TCAATGACTC CTGTTGTGGC GAGTGGTATT CCAGAGTCTA TCACACGTAG CTGTAGTGTA 
GGCTATCTAG ATAATATGGA AATTGTCCCT AGTGATGCTG TACTTACGAT GATGCGTAAA 
GAAACTCCTT ATAAACGGTT AGTTTTGGTT GATAAGAAGC GGCAGAAAAA GTACCGTCGT 
AATTTTGATG ATATGAAGAA AGCAAAACTG CGCCAAGGTT GCAAATCTAA AAGTTTAGAC 
TCGTGCGACA TACTGCAGGA TTCGCCCAAT TTGAACCGAG ACCTGATAAA TCTTTTCCGG 
CAGATGCCCA AAGTGAACGA GCTTTCAGAG AATGAAACTG AATACTCTTC GTCGGATCAG 
ACCGATCGAA TGCGTCCGAA TGCGCCAACT GGATCATCCT CCGCAGCATC AACAAAACGA 
AGACCATCAA TGAAATTTCC AGGAGTAACA GGCCCTATTC TACCTATTTC CAAAACACCT 
ATTCTCAATC GGAAGGAGAA GCCAAAATCT TGTTCTATAT GTAAAACGAT TTCTCCGACT 
GTTAACTCCA CAGAAACAGT ATGTGTGAAG TGCCGAAAGA ATGGAGGAAC TTCCAATGGA 
GTAGACAAAG AAAATAATAG TATAGAAGAC CACCAAAACC ACATTTACGT AGATATAATA 
AGCGCGAAAC CTCCACAAGC TCCCCCCAAA GCCAAGCGAT CAATACTTGA GCAATACATT 
AGTTCAGTTT ATAGTAATAA CGGAGAATTG GTACTTAAGG ATACCTCTAG TGGAACCAGT 
GGAACGAAAA CTACTAACGA CAAGAAAAAC AACAATAGTA ATGGAGATAC TAATGGATCG 
TGTAGCACGC CGAAGTCCAA AATTAGGAAA AATTTCGAGA TAGTTACAAG CTTTACCGAC 
AGTCCTCTAT TTAGTCGAAG ACATCGTTCC TTGAATAGAA CCGACTCGTC AAAATTGCAG 
AGTGAAAGCA GCACTCCAGT AATGGCTCGC AAGTCCGAAA CAAGTAAAAG TGAAAACGGC 
GAAAGTCGAA AAAGCAGACG AAAAGACGAA TCACCTCGGC GACATCGTCG CAACGATAGT 
TGTCCTGGTC CAAGTATAGA AAACAACAAT GGCGAGGGAC CATCCCTTCA ACCAGTGGAA 
ATTACTCCCG TTGAGCAGAA AGCTTCGGTT TCGCTTCACA CGCAAGCTTT GACCACCCTT 
GAAAACATCA TCAGCCGGCT GAGGGATCTT GACGAAGGCC GTCTAACGCC ACCCTCATCT 
CCGTCGAATT CTCGTTTGCC ACGCAGTTCA CCGGCTTCAC CTGCTCCGAG TAAAAAGAGC 
AAACGTCACC AAAGTGCTTC GCCTATTCGT CATATATTGA ATTCACCGCT ACTGAATCGG 
AGGCAGCGGA AGAAGCAGCA TACGGAAAGC TCTGACGATG AAAATCAATT GTTGACCCAG 
ACAGACGACA GTAGCGGAGG AAGTGGGAGT AACAACAACA AGCAGTATCG TGACTTGGAA 
ACGTTTCAAA AGGCTCAACT CCGACAAAAG CTAAAACGTG GTAAAATTGA GCCAAATGGA 
ATTACGAGTT CCTTTCAGCC GCAGGCTCCA GTGAGACGAG AATTCGTTAT GCACAATAAG 
GCTCCCATGT GGAATGAAAA CAGCCAAGTG TATCAACTGG ACTTCGGTGG ACGAGTGACC 
CAGGAGTCGG CTAAGAATTT CCAGATAGAA TTCCGTGGCA AACAGGTCAT GCAGTTCGGT 
CGGATTGACG GTAATGCGTA CACGTTGGAT TTCCAATATC CCTTTTCGGC ACTGCAAGCT 
TTCGCCGTCG CTCTAGCGAA CGTTACTCAG CGGTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5577522 ( XM_021855559.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711251.1 Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAe115725
Clone ID Related Accession (Same CDS sequence)	XM_021855559.1
Accession Version	XM_021855559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tubby-related protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGATTCAACG ATCACAACTT CAAAAAGTTC ATCAGCGTCC 
AGTCATTCGA CGAGTGGGAC CACCGGAAGA TCAGACAGTT CGGAAAGTGA TATAGAAGAA 
GGTTGTCGTT CGCCTCGATT GTCCAGAAAA CGAAAAGCAC GAACTAAAAA GCGACAACCG 
AGTAATAGCG ATCGCTCTCC TAATCAAAAC TCTGATTCTA CTGAGAATAA CGAGGAATTG 
GCATACCTGG ATACATTACC AGAGCATGTC AAACTGGTGG AAGTCACGTC AAATATCTGG 
GGAACTAAGT TTAAAATTCA CGGTTTAGCA AAAACACTTC CGGCAAATCT TGGTCAAGTT 
ACTTATAAAA CGTCTTTACT TCACCTACAA CCACGCCAAA TGACCTTAGT TATTACTGAA 
CTTCGGGATG ATTTCCCAAC TGGGCCGGAC CCGAACTTTA ATCCCAACAT ATTTTCAGAA 
GATGAAGAAG AACACAATCA AACTCATTTT GGTTCTGGTG GTCGCAAACT TGTAGAAACC 
GCTCCTCCGA TAGCCCCTAT GTCACCTCGT CCAAATCGAT TTCCTTCTCG ACCGCGTCGT 
CAAACACCTT CTTCGCTGGC ATCTGCCTCC AACGGTCGTC CTCCATTGCC TCATCTTGGC 
CCTCTAGCTC GTGCCGAATC CTATGAAGAT GACACCGATG GACCAGATGG AGAACCGTCA 
CGCACTGTTG CAAATCCTCC CCGTCAAGGA ATTAGTTATT CTAGTTTAGT TAGCCAATGC 
AGTAGACCAT CATCTGCGTC TAGTAGTCAA TCACGTGTTG CCATATCTCC ACTGTACTGC 
GAAGGTTCCG TTCCAACGCT ACAGTCACCG AAGAATGCTG TAGCTCCTAG TGATATTATT 
TTTGATAGAC CACCAGCCGG ACAAACTACC CTTATGAGTT ATTCAAGTAA TACGGATTAC 
GTTAACAATG TTGTTCAGGT GAAAAACGCC ATTATGTCCT CGGATCATAG TCGTACCAAC 
AATCAGTCTC ACGTCAACCC GGTACCATTA AATCTAAATC TCAATTTGGA ACGGATAGAA 
GCCAAAGCTA ACAATAGTAG TAGTAGAGAC TCGTCACATT CCACGGGTAG TGGTAGTAAG 
CATGGAACTC CGAAGAAGCA AAATTTAAAA TACATCGACG AAGAAACTCC ATCTACCTCT 
GCTACGCCAG TTGTTCCAGA GCCTAGCACC AGTGGAACTA GCAACTGCAG CAGTAACCCA 
TCTGGTGCTA TCATGAAACG CACTCCGACC GTAATTTCAA TGACTCCTGT TGTGGCGAGT 
GGTATTCCAG AGTCTATCAC ACGTAGCTGT AGTGTAGGCT ATCTAGATAA TATGGAAATT 
GTCCCTAGTG ATGCTGTACT TACGATGATG CGTAAAGAAA CTCCTTATAA ACGGTTAGTT 
TTGGTTGATA AGAAGCGGCA GAAAAAGTAC CGTCGTAATT TTGATGATAT GAAGAAAGCA 
AAACTGCGCC AAGGTTGCAA ATCTAAAAGT TTAGACTCGT GCGACATACT GCAGGATTCG 
CCCAATTTGA ACCGAGACCT GATAAATCTT TTCCGGCAGA TGCCCAAAGT GAACGAGCTT 
TCAGAGAATG AAACTGAATA CTCTTCGTCG GATCAGACCG ATCGAATGCG TCCGAATGCG 
CCAACTGGAT CATCCTCCGC AGCATCAACA AAACGAAGAC CATCAATGAA ATTTCCAGGA 
GTAACAGGCC CTATTCTACC TATTTCCAAA ACACCTATTC TCAATCGGAA GGAGAAGCCA 
AAATCTTGTT CTATATGTAA AACGATTTCT CCGACTGTTA ACTCCACAGA AACAGTATGT 
GTGAAGTGCC GAAAGAATGG AGGAACTTCC AATGGAGTAG ACAAAGAAAA TAATAGTATA 
GAAGACCACC AAAACCACAT TTACGTAGAT ATAATAAGCG CGAAACCTCC ACAAGCTCCC 
CCCAAAGCCA AGCGATCAAT ACTTGAGCAA TACATTAGTT CAGTTTATAG TAATAACGGA 
GAATTGGTAC TTAAGGATAC CTCTAGTGGA ACCAGTGGAA CGAAAACTAC TAACGACAAG 
AAAAACAACA ATAGTAATGG AGATACTAAT GGATCGTGTA GCACGCCGAA GTCCAAAATT 
AGGAAAAATT TCGAGATAGT TACAAGCTTT ACCGACAGTC CTCTATTTAG TCGAAGACAT 
CGTTCCTTGA ATAGAACCGA CTCGTCAAAA TTGCAGAGTG AAAGCAGCAC TCCAGTAATG 
GCTCGCAAGT CCGAAACAAG TAAAAGTGAA AACGGCGAAA GTCGAAAAAG CAGACGAAAA 
GACGAATCAC CTCGGCGACA TCGTCGCAAC GATAGTTGTC CTGGTCCAAG TATAGAAAAC 
AACAATGGCG AGGGACCATC CCTTCAACCA GTGGAAATTA CTCCCGTTGA GCAGAAAGCT 
TCGGTTTCGC TTCACACGCA AGCTTTGACC ACCCTTGAAA ACATCATCAG CCGGCTGAGG 
GATCTTGACG AAGGCCGTCT AACGCCACCC TCATCTCCGT CGAATTCTCG TTTGCCACGC 
AGTTCACCGG CTTCACCTGC TCCGAGTAAA AAGAGCAAAC GTCACCAAAG TGCTTCGCCT 
ATTCGTCATA TATTGAATTC ACCGCTACTG AATCGGAGGC AGCGGAAGAA GCAGCATACG 
GAAAGCTCTG ACGATGAAAA TCAATTGTTG ACCCAGACAG ACGACAGTAG CGGAGGAAGT 
GGGAGTAACA ACAACAAGCA GTATCGTGAC TTGGAAACGT TTCAAAAGGC TCAACTCCGA 
CAAAAGCTAA AACGTGGTAA AATTGAGCCA AATGGAATTA CGAGTTCCTT TCAGCCGCAG 
GCTCCAGTGA GACGAGAATT CGTTATGCAC AATAAGGCTC CCATGTGGAA TGAAAACAGC 
CAAGTGTATC AACTGGACTT CGGTGGACGA GTGACCCAGG AGTCGGCTAA GAATTTCCAG 
ATAGAATTCC GTGGCAAACA GGTCATGCAG TTCGGTCGGA TTGACGGTAA TGCGTACACG 
TTGGATTTCC AATATCCCTT TTCGGCACTG CAAGCTTTCG CCGTCGCTCT AGCGAACGTT 
ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711251.1
CDS	1409..5986
Translation	MHLHFEKSTNTKCDCSILSLSWMGKVPDDIPEEEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCKKNVEYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSVELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLDATITCGIWTPDDQQVYFGTTQGQIIVMDVHGAMVSQVQLGSDTGITAMAWSCEKFKMEEGEDTEPGVTNASKRSFVLAVSFQNGYIYLLKSFDDVSPCHIHTEMNGNLGFVMEWSNSRELLAVAGSEIAAESETTETPAMPVYNNLLKFYTESGNLLYTAKIPCTTTPVSALTWGHNDKRIFIATGTQVHIAWVSRRVASLQLFCRLQVQSSLTSEAMLPKLPLPGRIKALIGNLFAQTIRCCVPDLKYLREFVSRPPVCSTRLHCTMIRHDEDANQSSGTCYTLYLEFLGGLVPLLKGKRTSKIRPEFVIFDPQIDDSTITTSKSSSASSHSTSGTTGRSDSSESDIEEGCRSPRLSRKRKARTKKRQPSNSDRSPNQNSDSTENNEELAYLDTLPEHVKLVEVTSNIWGTKFKIHGLAKTLPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPTGPDPNFNPNIFSEDEEEHNQTHFGSGGRKLVETAPPIAPMSPRPNRFPSRPRRQTPSSLASASNGRPPLPHLGPLARAESYEDDTDGPDGEPSRTVANPPRQGISYSSLVSQCSRPSSASSSQSRVAISPLYCEGSVPTLQSPKNAVAPSDIIFDRPPAGQTTLMSYSSNTDYVNNVVQVKNAIMSSDHSRTNNQSHVNPVPLNLNLNLERIEAKANNSSSRDSSHSTGSGSKHGTPKKQNLKYIDEETPSTSATPVVPEPSTSGTSNCSSNPSGAIMKRTPTVISMTPVVASGIPESITRSCSVGYLDNMEIVPSDAVLTMMRKETPYKRLVLVDKKRQKKYRRNFDDMKKAKLRQGCKSKSLDSCDILQDSPNLNRDLINLFRQMPKVNELSENETEYSSSDQTDRMRPNAPTGSSSAASTKRRPSMKFPGVTGPILPISKTPILNRKEKPKSCSICKTISPTVNSTETVCVKCRKNGGTSNGVDKENNSIEDHQNHIYVDIISAKPPQAPPKAKRSILEQYISSVYSNNGELVLKDTSSGTSGTKTTNDKKNNNSNGDTNGSCSTPKSKIRKNFEIVTSFTDSPLFSRRHRSLNRTDSSKLQSESSTPVMARKSETSKSENGESRKSRRKDESPRRHRRNDSCPGPSIENNNGEGPSLQPVEITPVEQKASVSLHTQALTTLENIISRLRDLDEGRLTPPSSPSNSRLPRSSPASPAPSKKSKRHQSASPIRHILNSPLLNRRQRKKQHTESSDDENQLLTQTDDSSGGSGSNNNKQYRDLETFQKAQLRQKLKRGKIEPNGITSSFQPQAPVRREFVMHNKAPMWNENSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_021855559.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tubby-related protein 4 (LOC5577522), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855559.1

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGAA AAGCACCAAC ACGAAATGCG ACTGCTCGAT ACTTTCGCTG 
TCATGGATGG GAAAAGTTCC AGACGATATT CCAGAGGAGG AAGGATGGAA GTTGAACCGT 
ACGAACTACT ATCAGGAAGG ATGGCTAGCA ACTGGCAACG TGCGAGGCAT CGTTGGAGTT 
ACCTTCACAA CGTCACACTG CAAGAAGAAC GTCGAATATC CCCTGAGGAC AAACTACAAC 
CTGCGAGGGC ACCGCTCAGA CGTGATTCTG GTGAAATGGA ACGAGCCGTA CCAGAAGTTG 
GCAAGCTGTG ATAGCTCCGG TATCATCTTC GTGTGGATAA AATACGAAGG ACGATGGAGC 
GTGGAGCTAA TAAACGACAG AAACACGCCG GTAACACATT TTTCCTGGTC ACACGACGGA 
AGGATGGCAC TAATATGCTA TCAGGATGGT TTCGTGTTGG TTGGCTCCGT CGCTGGACAA 
CGGTACTGGT CATCAATGCT GAACCTGGAT GCTACTATCA CGTGCGGAAT CTGGACGCCA 
GACGATCAGC AGGTGTATTT TGGCACAACG CAGGGCCAGA TAATTGTGAT GGACGTGCAC 
GGGGCGATGG TTTCACAGGT TCAGCTTGGT TCGGATACCG GAATCACTGC AATGGCATGG 
TCATGCGAAA AGTTCAAAAT GGAGGAAGGT GAAGACACGG AACCAGGAGT TACAAATGCT 
TCCAAACGCT CTTTTGTGTT AGCAGTAAGC TTCCAGAATG GATACATTTA CTTACTGAAG 
TCTTTCGATG ATGTTTCGCC TTGTCACATA CATACGGAAA TGAACGGAAA TCTAGGCTTT 
GTGATGGAGT GGAGTAATTC GCGTGAGTTA TTGGCTGTGG CTGGTAGTGA AATAGCCGCT 
GAATCGGAAA CGACTGAAAC ACCTGCAATG CCAGTGTACA ATAATCTTCT GAAGTTTTAC 
ACAGAATCAG GAAATCTACT ATACACAGCT AAAATACCTT GCACTACGAC TCCAGTATCT 
GCCCTAACGT GGGGTCACAA CGACAAACGA ATATTTATTG CTACGGGAAC ACAGGTTCAC 
ATCGCATGGG TGTCGCGAAG GGTTGCATCT TTGCAATTGT TTTGTCGACT ACAAGTACAA 
TCTTCGCTAA CTTCAGAAGC CATGCTGCCC AAATTACCAC TACCAGGTCG AATCAAAGCA 
CTGATTGGCA ATCTTTTCGC CCAAACCATT CGGTGCTGTG TTCCGGATCT GAAATATTTA 
CGAGAATTTG TGTCTCGACC ACCAGTGTGT TCGACCCGGT TACATTGTAC GATGATCCGA 
CATGATGAAG ACGCAAATCA AAGTTCTGGT ACTTGTTACA CATTGTACCT GGAATTTCTT 
GGAGGTTTAG TTCCGCTTCT CAAAGGCAAA AGGACGTCGA AAATCCGGCC GGAATTTGTG 
ATATTTGATC CACAGATCGA TGATTCAACG ATCACAACTT CAAAAAGTTC ATCAGCGTCC 
AGTCATTCGA CGAGTGGGAC CACCGGAAGA TCAGACAGTT CGGAAAGTGA TATAGAAGAA 
GGTTGTCGTT CGCCTCGATT GTCCAGAAAA CGAAAAGCAC GAACTAAAAA GCGACAACCG 
AGTAATAGCG ATCGCTCTCC TAATCAAAAC TCTGATTCTA CTGAGAATAA CGAGGAATTG 
GCATACCTGG ATACATTACC AGAGCATGTC AAACTGGTGG AAGTCACGTC AAATATCTGG 
GGAACTAAGT TTAAAATTCA CGGTTTAGCA AAAACACTTC CGGCAAATCT TGGTCAAGTT 
ACTTATAAAA CGTCTTTACT TCACCTACAA CCACGCCAAA TGACCTTAGT TATTACTGAA 
CTTCGGGATG ATTTCCCAAC TGGGCCGGAC CCGAACTTTA ATCCCAACAT ATTTTCAGAA 
GATGAAGAAG AACACAATCA AACTCATTTT GGTTCTGGTG GTCGCAAACT TGTAGAAACC 
GCTCCTCCGA TAGCCCCTAT GTCACCTCGT CCAAATCGAT TTCCTTCTCG ACCGCGTCGT 
CAAACACCTT CTTCGCTGGC ATCTGCCTCC AACGGTCGTC CTCCATTGCC TCATCTTGGC 
CCTCTAGCTC GTGCCGAATC CTATGAAGAT GACACCGATG GACCAGATGG AGAACCGTCA 
CGCACTGTTG CAAATCCTCC CCGTCAAGGA ATTAGTTATT CTAGTTTAGT TAGCCAATGC 
AGTAGACCAT CATCTGCGTC TAGTAGTCAA TCACGTGTTG CCATATCTCC ACTGTACTGC 
GAAGGTTCCG TTCCAACGCT ACAGTCACCG AAGAATGCTG TAGCTCCTAG TGATATTATT 
TTTGATAGAC CACCAGCCGG ACAAACTACC CTTATGAGTT ATTCAAGTAA TACGGATTAC 
GTTAACAATG TTGTTCAGGT GAAAAACGCC ATTATGTCCT CGGATCATAG TCGTACCAAC 
AATCAGTCTC ACGTCAACCC GGTACCATTA AATCTAAATC TCAATTTGGA ACGGATAGAA 
GCCAAAGCTA ACAATAGTAG TAGTAGAGAC TCGTCACATT CCACGGGTAG TGGTAGTAAG 
CATGGAACTC CGAAGAAGCA AAATTTAAAA TACATCGACG AAGAAACTCC ATCTACCTCT 
GCTACGCCAG TTGTTCCAGA GCCTAGCACC AGTGGAACTA GCAACTGCAG CAGTAACCCA 
TCTGGTGCTA TCATGAAACG CACTCCGACC GTAATTTCAA TGACTCCTGT TGTGGCGAGT 
GGTATTCCAG AGTCTATCAC ACGTAGCTGT AGTGTAGGCT ATCTAGATAA TATGGAAATT 
GTCCCTAGTG ATGCTGTACT TACGATGATG CGTAAAGAAA CTCCTTATAA ACGGTTAGTT 
TTGGTTGATA AGAAGCGGCA GAAAAAGTAC CGTCGTAATT TTGATGATAT GAAGAAAGCA 
AAACTGCGCC AAGGTTGCAA ATCTAAAAGT TTAGACTCGT GCGACATACT GCAGGATTCG 
CCCAATTTGA ACCGAGACCT GATAAATCTT TTCCGGCAGA TGCCCAAAGT GAACGAGCTT 
TCAGAGAATG AAACTGAATA CTCTTCGTCG GATCAGACCG ATCGAATGCG TCCGAATGCG 
CCAACTGGAT CATCCTCCGC AGCATCAACA AAACGAAGAC CATCAATGAA ATTTCCAGGA 
GTAACAGGCC CTATTCTACC TATTTCCAAA ACACCTATTC TCAATCGGAA GGAGAAGCCA 
AAATCTTGTT CTATATGTAA AACGATTTCT CCGACTGTTA ACTCCACAGA AACAGTATGT 
GTGAAGTGCC GAAAGAATGG AGGAACTTCC AATGGAGTAG ACAAAGAAAA TAATAGTATA 
GAAGACCACC AAAACCACAT TTACGTAGAT ATAATAAGCG CGAAACCTCC ACAAGCTCCC 
CCCAAAGCCA AGCGATCAAT ACTTGAGCAA TACATTAGTT CAGTTTATAG TAATAACGGA 
GAATTGGTAC TTAAGGATAC CTCTAGTGGA ACCAGTGGAA CGAAAACTAC TAACGACAAG 
AAAAACAACA ATAGTAATGG AGATACTAAT GGATCGTGTA GCACGCCGAA GTCCAAAATT 
AGGAAAAATT TCGAGATAGT TACAAGCTTT ACCGACAGTC CTCTATTTAG TCGAAGACAT 
CGTTCCTTGA ATAGAACCGA CTCGTCAAAA TTGCAGAGTG AAAGCAGCAC TCCAGTAATG 
GCTCGCAAGT CCGAAACAAG TAAAAGTGAA AACGGCGAAA GTCGAAAAAG CAGACGAAAA 
GACGAATCAC CTCGGCGACA TCGTCGCAAC GATAGTTGTC CTGGTCCAAG TATAGAAAAC 
AACAATGGCG AGGGACCATC CCTTCAACCA GTGGAAATTA CTCCCGTTGA GCAGAAAGCT 
TCGGTTTCGC TTCACACGCA AGCTTTGACC ACCCTTGAAA ACATCATCAG CCGGCTGAGG 
GATCTTGACG AAGGCCGTCT AACGCCACCC TCATCTCCGT CGAATTCTCG TTTGCCACGC 
AGTTCACCGG CTTCACCTGC TCCGAGTAAA AAGAGCAAAC GTCACCAAAG TGCTTCGCCT 
ATTCGTCATA TATTGAATTC ACCGCTACTG AATCGGAGGC AGCGGAAGAA GCAGCATACG 
GAAAGCTCTG ACGATGAAAA TCAATTGTTG ACCCAGACAG ACGACAGTAG CGGAGGAAGT 
GGGAGTAACA ACAACAAGCA GTATCGTGAC TTGGAAACGT TTCAAAAGGC TCAACTCCGA 
CAAAAGCTAA AACGTGGTAA AATTGAGCCA AATGGAATTA CGAGTTCCTT TCAGCCGCAG 
GCTCCAGTGA GACGAGAATT CGTTATGCAC AATAAGGCTC CCATGTGGAA TGAAAACAGC 
CAAGTGTATC AACTGGACTT CGGTGGACGA GTGACCCAGG AGTCGGCTAA GAATTTCCAG 
ATAGAATTCC GTGGCAAACA GGTCATGCAG TTCGGTCGGA TTGACGGTAA TGCGTACACG 
TTGGATTTCC AATATCCCTT TTCGGCACTG CAAGCTTTCG CCGTCGCTCT AGCGAACGTT 
ACTCAGCGGT TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5577522 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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