LOC100165283 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100165283
Entrez Gene ID	100165283
Full Name	bifunctional glutamate/proline--tRNA ligase
Gene Type	protein-coding
Organism	Acyrthosiphon pisum(pea aphid)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029485418.1	XP_029341278.1	bifunctional glutamate/proline--tRNA ligase isoform X2
XM_001946225.4	XP_001946260.2	bifunctional glutamate/proline--tRNA ligase
XM_001946225.5	XP_001946260.2	bifunctional glutamate/proline--tRNA ligase isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100165283 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100165283 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAd16962	XM_001946225.5 Latest version!	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAd29969	XM_029485418.1 Latest version!	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OAd16962	XM_001946225.4	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100165283 ( XM_001946225.4 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_001946260.2 Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), mRNA.

Price & Availability↑

CloneID	OAd16962
Clone ID Related Accession (Same CDS sequence)	XM_001946225.4 , XM_001946225.5
Accession Version	XM_001946225.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463328000000
Organism	Acyrthosiphon pisum(pea aphid)
Product	bifunctional glutamate/proline--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003384880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2016 this sequence version replaced XM_001946225.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acyrthosiphon pisum Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TTGCAGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCACA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCAGGTGGT 
ATTCCCACAC CAAAGCTAGT CAGTAACGCT AGTTGTGATA AAAGTAAACC TTCTGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAATAAA 
GCTTCTAAAG CAGACATCGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAAGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATTGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTGA TCAAAGTAAA 
CCTTCAGTAG ATTCTATAGT TGTGAAAATT AATGAACAGG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGAGTT GATTTTCCTG CGGGTGGTAT TCCCACACCA 
CAGCTAGTCA GTAATGCCAA TTCTAATCAA AGTAAACCTT TAGTAGATTC TATAGTTGTG 
AAAATTAATG AACAAGGAGA TAAAGTGCGA TCAATGAAAA ACAGTAAAGC TTCTAAAGCA 
GACATCGGGG AAGCTGTCAA AGTCTTACTT GCTTTGAAAG CAGAGTATAA AAGTATTGCT 
GGGGTTGATT TTCCTGCAAG TGGAATTCCC ACACCACAAC AAGCAGATAA ATGTAACTCC 
GAGTTAAGTA AACCTTCTTC TGCTGATGCA GTAGTACAGC ATATAAATGA ACAAGGTGAA 
AAAACAATGT TGACGAAAAA CAGTAATACT TCCAAAGATA AAAAATCGAA GAAAAAAGAG 
AATCTTGCTG TGGCCACTAC TAAAACATCT GATAGTACAA ACACCAAAAA AGTAACAAAA 
TTAGGGTTGG AAGTATTAAA GGTATCCGAA AATCTTGGAG AATGGTATTC TCAGGTATTG 
GTTAAAGGCG AAATGATTGA ATATTATGAT GTAAGTGGAT GTTACGTGCT CAGGCCTTGG 
GCTTACAGAA TATGGGAATT AATACAAGAA TGGTTCGATG CACGAATTAA GAGAAAGCCT 
CTTAATATAC GGAATTGTTA TTTCCCAATG TTTGTATCAC AAGGAGCATT AGAAAGAGAA 
AAAGAACATA TTGCAGATTT TGCACCAGAG GTAGCTTGGG TTACCAAATC TGGTGAAACG 
GATTTAGCTG AACCTATTGC TATAAGACCA ACTAGTGAGA CTGGGATGTA TCCAGCTTTT 
GCGAAATGGA TTCAGTCGTA CAGAGACTTG CCATTGAAAC TCAATCAATG GAACAGCGTT 
GTGAGGTGGG AATTCAAACA CCCTCAACCC TTCTTGAGAA CTAGAGAGTT TTTGTGGCAA 
GAGGGTCATA ATGCGTATGC AACTAGAGAA GAAGCAGAAC GTGATGTATT AATAACATTA 
GGTTATTATG AGTATGTCTA CAAAAATATG CTAGCAGTAC CAGTGATAAA AGGTAAAAAA 
ACTGAAAAAG AAAAGTTTGC TGGAGGAATA TTTACTACTA CTTGTGAAGC ATATGTAGCA 
TCTAGTGGTC GCGCAATTCA AGGTGCCACC TCACATTATC TGGGTACCAA TTTTGCTAAA 
ATGTTTGATG TTCATTTTGA ACATCCAGAC ACACACGAGA AATCATTTGT TCATCAAATT 
TCATTTGGAA TGACAACTAG AACTATTGGT GTTATGGTAA TGGTTCATGG TGATGACCGT 
GGGCTGGTTT TACCTCCACG CATAGCATCT ATTCAAGTAA TTATTGTACC ATGTGGTGTA 
GGCGTTAGCA CAGCAAACGA AGTCAAAGAT AATTTGATTA GAACTTGTTT AGATGTATGT 
AACAGATTAA AAGGAGATAG TCTGATAGAA ACTGATGATG AAAATGACAA TAGTGTTATC 
CAACATCGTA ATTCTTCCTT AATGATAAGA GCAGATACCG ACTTGCGAGA TAATTATTCA 
CCTGGATGGA AGTTCAACCA CTGGGAGTTG AAGGGGGTTC CGTTGAGGTT AGAAATTGGA 
CCAAAAGATT TGGATCGAAA CCAAATGGTA GCTGTGCGGC GAGATAATGG AGATAAACTA 
ACTATTCCCT TAGAGGGACG TCCAAATGAT GGTGGAAACG ACAAGATGGT TGTAAGAATA 
ACCGAGCTGT TGAATCAAAT ACAACAAGAT ATGTACAATA AAGCAGAACA AGAAATGAAA 
GAAAATGTTG TGATATGCCG TAAATGGTCA GAGTGTGCTG CGCAGTTAGC AGCAAAACAT 
TTATTATTGA TACCGTTTTG TGGACGTCCT ATTTGCGAGG ATAACATCAA ACGGGATACC 
ACATTGGACG ATGGTACTGC GGATGTTGGA GCTTCGACAA TGGGAGCAAA GTCGTTATGT 
GTGCCATTTG AACAGCCCAA GGGAGCAGAT ATATTGAAAT CCAATGACCA CTGTATACAT 
CCAGAATGTG GGCAGCCAGC TGGGTCTTTC ACTCTATTTG GCCGCAGTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001946260.2
CDS	190..5163
Translation	MSNAPEKDLTSLEQQLATPDLQSTNAKKQGSTSVLKSKKTETKKNEKAPKLQKSVEDNSKQQGKFVELPGAEMGKVVVRFPPEASGYLHIGHAKAALLNQHYQLAFNGRLIMRFDDTNPAKEKEDFEKVILEDVDMLKIKPDIFTYTSDHFEKMLDICENGMLKEGLAYVDDTDGDKIRLEREQRIDSINRNNSIEKNLELWKQMVLGTEIGLKCCVRAKIDMQSANGCLRDPTIYRCKPEHHPRTGDKYKVYPTYDFACPIVDSFEGVTHCLRTTEYTDRDAQYYWFCDALKLGTRSNETRPYVWSYSRLNMTNTVLSKRKLTWFVEQGHVDGWDDPRMPTVRGVFRRGMQVEALKQFIVAQGSSRTVTAMQWDKIWSLNRSIVDSIAPRHTALLLSNSNKENVVLSYPPVEVVVENVEKPYKIDNVPKHPKNSQLGLKNCVWAAPKILIDYSDAELLEAGRRATFINWGNLLINRIERNQNNNKIERIHAVSDLENTDFKSTVKVTWLPKTDYSESEEVLPILVPVVCVYFDHLISKAVLAKDDDFKQYLNTENSRWELQMIGDSELKDLKKGDVIQLQRRGYFICDSTYQPYSIHTGKETPLVLFNIPDGHTKSATPITPVPLPITTSNIKKNTNKDIDTVISQINDQANKINVLTADLEEAGKVLHALKAEYKACTGIDFPATGISTPQTVSNSVSGQSNPSADAIVLKINEQGDIVRSMKNNKASKADIAEAVKVLLALKAQYKSIAGVDFPAGGIPTPKLVSNASCDKSKPSVDSIVVKINEQGDKVRSMKNNKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSNQSKPLVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPASGIPTPQQADKCNSELSKPSSADAVVQHINEQGEKTMLTKNSNTSKDKKSKKKENLAVATTKTSDSTNTKKVTKLGLEVLKVSENLGEWYSQVLVKGEMIEYYDVSGCYVLRPWAYRIWELIQEWFDARIKRKPLNIRNCYFPMFVSQGALEREKEHIADFAPEVAWVTKSGETDLAEPIAIRPTSETGMYPAFAKWIQSYRDLPLKLNQWNSVVRWEFKHPQPFLRTREFLWQEGHNAYATREEAERDVLITLGYYEYVYKNMLAVPVIKGKKTEKEKFAGGIFTTTCEAYVASSGRAIQGATSHYLGTNFAKMFDVHFEHPDTHEKSFVHQISFGMTTRTIGVMVMVHGDDRGLVLPPRIASIQVIIVPCGVGVSTANEVKDNLIRTCLDVCNRLKGDSLIETDDENDNSVIQHRNSSLMIRADTDLRDNYSPGWKFNHWELKGVPLRLEIGPKDLDRNQMVAVRRDNGDKLTIPLEGRPNDGGNDKMVVRITELLNQIQQDMYNKAEQEMKENVVICRKWSECAAQLAAKHLLLIPFCGRPICEDNIKRDTTLDDGTADVGASTMGAKSLCVPFEQPKGADILKSNDHCIHPECGQPAGSFTLFGRSY

Target ORF information:

RefSeq Version	XM_001946225.4
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001946225.4

ORF Insert Sequence:

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TTGCAGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCACA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCAGGTGGT 
ATTCCCACAC CAAAGCTAGT CAGTAACGCT AGTTGTGATA AAAGTAAACC TTCTGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAATAAA 
GCTTCTAAAG CAGACATCGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAAGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATTGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTGA TCAAAGTAAA 
CCTTCAGTAG ATTCTATAGT TGTGAAAATT AATGAACAGG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGAGTT GATTTTCCTG CGGGTGGTAT TCCCACACCA 
CAGCTAGTCA GTAATGCCAA TTCTAATCAA AGTAAACCTT TAGTAGATTC TATAGTTGTG 
AAAATTAATG AACAAGGAGA TAAAGTGCGA TCAATGAAAA ACAGTAAAGC TTCTAAAGCA 
GACATCGGGG AAGCTGTCAA AGTCTTACTT GCTTTGAAAG CAGAGTATAA AAGTATTGCT 
GGGGTTGATT TTCCTGCAAG TGGAATTCCC ACACCACAAC AAGCAGATAA ATGTAACTCC 
GAGTTAAGTA AACCTTCTTC TGCTGATGCA GTAGTACAGC ATATAAATGA ACAAGGTGAA 
AAAACAATGT TGACGAAAAA CAGTAATACT TCCAAAGATA AAAAATCGAA GAAAAAAGAG 
AATCTTGCTG TGGCCACTAC TAAAACATCT GATAGTACAA ACACCAAAAA AGTAACAAAA 
TTAGGGTTGG AAGTATTAAA GGTATCCGAA AATCTTGGAG AATGGTATTC TCAGGTATTG 
GTTAAAGGCG AAATGATTGA ATATTATGAT GTAAGTGGAT GTTACGTGCT CAGGCCTTGG 
GCTTACAGAA TATGGGAATT AATACAAGAA TGGTTCGATG CACGAATTAA GAGAAAGCCT 
CTTAATATAC GGAATTGTTA TTTCCCAATG TTTGTATCAC AAGGAGCATT AGAAAGAGAA 
AAAGAACATA TTGCAGATTT TGCACCAGAG GTAGCTTGGG TTACCAAATC TGGTGAAACG 
GATTTAGCTG AACCTATTGC TATAAGACCA ACTAGTGAGA CTGGGATGTA TCCAGCTTTT 
GCGAAATGGA TTCAGTCGTA CAGAGACTTG CCATTGAAAC TCAATCAATG GAACAGCGTT 
GTGAGGTGGG AATTCAAACA CCCTCAACCC TTCTTGAGAA CTAGAGAGTT TTTGTGGCAA 
GAGGGTCATA ATGCGTATGC AACTAGAGAA GAAGCAGAAC GTGATGTATT AATAACATTA 
GGTTATTATG AGTATGTCTA CAAAAATATG CTAGCAGTAC CAGTGATAAA AGGTAAAAAA 
ACTGAAAAAG AAAAGTTTGC TGGAGGAATA TTTACTACTA CTTGTGAAGC ATATGTAGCA 
TCTAGTGGTC GCGCAATTCA AGGTGCCACC TCACATTATC TGGGTACCAA TTTTGCTAAA 
ATGTTTGATG TTCATTTTGA ACATCCAGAC ACACACGAGA AATCATTTGT TCATCAAATT 
TCATTTGGAA TGACAACTAG AACTATTGGT GTTATGGTAA TGGTTCATGG TGATGACCGT 
GGGCTGGTTT TACCTCCACG CATAGCATCT ATTCAAGTAA TTATTGTACC ATGTGGTGTA 
GGCGTTAGCA CAGCAAACGA AGTCAAAGAT AATTTGATTA GAACTTGTTT AGATGTATGT 
AACAGATTAA AAGGAGATAG TCTGATAGAA ACTGATGATG AAAATGACAA TAGTGTTATC 
CAACATCGTA ATTCTTCCTT AATGATAAGA GCAGATACCG ACTTGCGAGA TAATTATTCA 
CCTGGATGGA AGTTCAACCA CTGGGAGTTG AAGGGGGTTC CGTTGAGGTT AGAAATTGGA 
CCAAAAGATT TGGATCGAAA CCAAATGGTA GCTGTGCGGC GAGATAATGG AGATAAACTA 
ACTATTCCCT TAGAGGGACG TCCAAATGAT GGTGGAAACG ACAAGATGGT TGTAAGAATA 
ACCGAGCTGT TGAATCAAAT ACAACAAGAT ATGTACAATA AAGCAGAACA AGAAATGAAA 
GAAAATGTTG TGATATGCCG TAAATGGTCA GAGTGTGCTG CGCAGTTAGC AGCAAAACAT 
TTATTATTGA TACCGTTTTG TGGACGTCCT ATTTGCGAGG ATAACATCAA ACGGGATACC 
ACATTGGACG ATGGTACTGC GGATGTTGGA GCTTCGACAA TGGGAGCAAA GTCGTTATGT 
GTGCCATTTG AACAGCCCAA GGGAGCAGAT ATATTGAAAT CCAATGACCA CTGTATACAT 
CCAGAATGTG GGCAGCCAGC TGGGTCTTTC ACTCTATTTG GCCGCAGTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100165283 ( XM_001946225.5 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_001946260.2 Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAd16962
Clone ID Related Accession (Same CDS sequence)	XM_001946225.4 , XM_001946225.5
Accession Version	XM_001946225.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2019 this sequence version replaced XM_001946225.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TTGCAGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCACA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCAGGTGGT 
ATTCCCACAC CAAAGCTAGT CAGTAACGCT AGTTGTGATA AAAGTAAACC TTCTGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAATAAA 
GCTTCTAAAG CAGACATCGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAAGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATTGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTGA TCAAAGTAAA 
CCTTCAGTAG ATTCTATAGT TGTGAAAATT AATGAACAGG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGAGTT GATTTTCCTG CGGGTGGTAT TCCCACACCA 
CAGCTAGTCA GTAATGCCAA TTCTAATCAA AGTAAACCTT TAGTAGATTC TATAGTTGTG 
AAAATTAATG AACAAGGAGA TAAAGTGCGA TCAATGAAAA ACAGTAAAGC TTCTAAAGCA 
GACATCGGGG AAGCTGTCAA AGTCTTACTT GCTTTGAAAG CAGAGTATAA AAGTATTGCT 
GGGGTTGATT TTCCTGCAAG TGGAATTCCC ACACCACAAC AAGCAGATAA ATGTAACTCC 
GAGTTAAGTA AACCTTCTTC TGCTGATGCA GTAGTACAGC ATATAAATGA ACAAGGTGAA 
AAAACAATGT TGACGAAAAA CAGTAATACT TCCAAAGATA AAAAATCGAA GAAAAAAGAG 
AATCTTGCTG TGGCCACTAC TAAAACATCT GATAGTACAA ACACCAAAAA AGTAACAAAA 
TTAGGGTTGG AAGTATTAAA GGTATCCGAA AATCTTGGAG AATGGTATTC TCAGGTATTG 
GTTAAAGGCG AAATGATTGA ATATTATGAT GTAAGTGGAT GTTACGTGCT CAGGCCTTGG 
GCTTACAGAA TATGGGAATT AATACAAGAA TGGTTCGATG CACGAATTAA GAGAAAGCCT 
CTTAATATAC GGAATTGTTA TTTCCCAATG TTTGTATCAC AAGGAGCATT AGAAAGAGAA 
AAAGAACATA TTGCAGATTT TGCACCAGAG GTAGCTTGGG TTACCAAATC TGGTGAAACG 
GATTTAGCTG AACCTATTGC TATAAGACCA ACTAGTGAGA CTGGGATGTA TCCAGCTTTT 
GCGAAATGGA TTCAGTCGTA CAGAGACTTG CCATTGAAAC TCAATCAATG GAACAGCGTT 
GTGAGGTGGG AATTCAAACA CCCTCAACCC TTCTTGAGAA CTAGAGAGTT TTTGTGGCAA 
GAGGGTCATA ATGCGTATGC AACTAGAGAA GAAGCAGAAC GTGATGTATT AATAACATTA 
GGTTATTATG AGTATGTCTA CAAAAATATG CTAGCAGTAC CAGTGATAAA AGGTAAAAAA 
ACTGAAAAAG AAAAGTTTGC TGGAGGAATA TTTACTACTA CTTGTGAAGC ATATGTAGCA 
TCTAGTGGTC GCGCAATTCA AGGTGCCACC TCACATTATC TGGGTACCAA TTTTGCTAAA 
ATGTTTGATG TTCATTTTGA ACATCCAGAC ACACACGAGA AATCATTTGT TCATCAAATT 
TCATTTGGAA TGACAACTAG AACTATTGGT GTTATGGTAA TGGTTCATGG TGATGACCGT 
GGGCTGGTTT TACCTCCACG CATAGCATCT ATTCAAGTAA TTATTGTACC ATGTGGTGTA 
GGCGTTAGCA CAGCAAACGA AGTCAAAGAT AATTTGATTA GAACTTGTTT AGATGTATGT 
AACAGATTAA AAGGAGATAG TCTGATAGAA ACTGATGATG AAAATGACAA TAGTGTTATC 
CAACATCGTA ATTCTTCCTT AATGATAAGA GCAGATACCG ACTTGCGAGA TAATTATTCA 
CCTGGATGGA AGTTCAACCA CTGGGAGTTG AAGGGGGTTC CGTTGAGGTT AGAAATTGGA 
CCAAAAGATT TGGATCGAAA CCAAATGGTA GCTGTGCGGC GAGATAATGG AGATAAACTA 
ACTATTCCCT TAGAGGGACG TCCAAATGAT GGTGGAAACG ACAAGATGGT TGTAAGAATA 
ACCGAGCTGT TGAATCAAAT ACAACAAGAT ATGTACAATA AAGCAGAACA AGAAATGAAA 
GAAAATGTTG TGATATGCCG TAAATGGTCA GAGTGTGCTG CGCAGTTAGC AGCAAAACAT 
TTATTATTGA TACCGTTTTG TGGACGTCCT ATTTGCGAGG ATAACATCAA ACGGGATACC 
ACATTGGACG ATGGTACTGC GGATGTTGGA GCTTCGACAA TGGGAGCAAA GTCGTTATGT 
GTGCCATTTG AACAGCCCAA GGGAGCAGAT ATATTGAAAT CCAATGACCA CTGTATACAT 
CCAGAATGTG GGCAGCCAGC TGGGTCTTTC ACTCTATTTG GCCGCAGTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001946260.2
CDS	218..5191
Translation	MSNAPEKDLTSLEQQLATPDLQSTNAKKQGSTSVLKSKKTETKKNEKAPKLQKSVEDNSKQQGKFVELPGAEMGKVVVRFPPEASGYLHIGHAKAALLNQHYQLAFNGRLIMRFDDTNPAKEKEDFEKVILEDVDMLKIKPDIFTYTSDHFEKMLDICENGMLKEGLAYVDDTDGDKIRLEREQRIDSINRNNSIEKNLELWKQMVLGTEIGLKCCVRAKIDMQSANGCLRDPTIYRCKPEHHPRTGDKYKVYPTYDFACPIVDSFEGVTHCLRTTEYTDRDAQYYWFCDALKLGTRSNETRPYVWSYSRLNMTNTVLSKRKLTWFVEQGHVDGWDDPRMPTVRGVFRRGMQVEALKQFIVAQGSSRTVTAMQWDKIWSLNRSIVDSIAPRHTALLLSNSNKENVVLSYPPVEVVVENVEKPYKIDNVPKHPKNSQLGLKNCVWAAPKILIDYSDAELLEAGRRATFINWGNLLINRIERNQNNNKIERIHAVSDLENTDFKSTVKVTWLPKTDYSESEEVLPILVPVVCVYFDHLISKAVLAKDDDFKQYLNTENSRWELQMIGDSELKDLKKGDVIQLQRRGYFICDSTYQPYSIHTGKETPLVLFNIPDGHTKSATPITPVPLPITTSNIKKNTNKDIDTVISQINDQANKINVLTADLEEAGKVLHALKAEYKACTGIDFPATGISTPQTVSNSVSGQSNPSADAIVLKINEQGDIVRSMKNNKASKADIAEAVKVLLALKAQYKSIAGVDFPAGGIPTPKLVSNASCDKSKPSVDSIVVKINEQGDKVRSMKNNKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSNQSKPLVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPASGIPTPQQADKCNSELSKPSSADAVVQHINEQGEKTMLTKNSNTSKDKKSKKKENLAVATTKTSDSTNTKKVTKLGLEVLKVSENLGEWYSQVLVKGEMIEYYDVSGCYVLRPWAYRIWELIQEWFDARIKRKPLNIRNCYFPMFVSQGALEREKEHIADFAPEVAWVTKSGETDLAEPIAIRPTSETGMYPAFAKWIQSYRDLPLKLNQWNSVVRWEFKHPQPFLRTREFLWQEGHNAYATREEAERDVLITLGYYEYVYKNMLAVPVIKGKKTEKEKFAGGIFTTTCEAYVASSGRAIQGATSHYLGTNFAKMFDVHFEHPDTHEKSFVHQISFGMTTRTIGVMVMVHGDDRGLVLPPRIASIQVIIVPCGVGVSTANEVKDNLIRTCLDVCNRLKGDSLIETDDENDNSVIQHRNSSLMIRADTDLRDNYSPGWKFNHWELKGVPLRLEIGPKDLDRNQMVAVRRDNGDKLTIPLEGRPNDGGNDKMVVRITELLNQIQQDMYNKAEQEMKENVVICRKWSECAAQLAAKHLLLIPFCGRPICEDNIKRDTTLDDGTADVGASTMGAKSLCVPFEQPKGADILKSNDHCIHPECGQPAGSFTLFGRSY

Target ORF information:

RefSeq Version	XM_001946225.5
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001946225.5

ORF Insert Sequence:

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TTGCAGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCACA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCAGGTGGT 
ATTCCCACAC CAAAGCTAGT CAGTAACGCT AGTTGTGATA AAAGTAAACC TTCTGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAATAAA 
GCTTCTAAAG CAGACATCGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAAGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATTGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTGA TCAAAGTAAA 
CCTTCAGTAG ATTCTATAGT TGTGAAAATT AATGAACAGG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGAGTT GATTTTCCTG CGGGTGGTAT TCCCACACCA 
CAGCTAGTCA GTAATGCCAA TTCTAATCAA AGTAAACCTT TAGTAGATTC TATAGTTGTG 
AAAATTAATG AACAAGGAGA TAAAGTGCGA TCAATGAAAA ACAGTAAAGC TTCTAAAGCA 
GACATCGGGG AAGCTGTCAA AGTCTTACTT GCTTTGAAAG CAGAGTATAA AAGTATTGCT 
GGGGTTGATT TTCCTGCAAG TGGAATTCCC ACACCACAAC AAGCAGATAA ATGTAACTCC 
GAGTTAAGTA AACCTTCTTC TGCTGATGCA GTAGTACAGC ATATAAATGA ACAAGGTGAA 
AAAACAATGT TGACGAAAAA CAGTAATACT TCCAAAGATA AAAAATCGAA GAAAAAAGAG 
AATCTTGCTG TGGCCACTAC TAAAACATCT GATAGTACAA ACACCAAAAA AGTAACAAAA 
TTAGGGTTGG AAGTATTAAA GGTATCCGAA AATCTTGGAG AATGGTATTC TCAGGTATTG 
GTTAAAGGCG AAATGATTGA ATATTATGAT GTAAGTGGAT GTTACGTGCT CAGGCCTTGG 
GCTTACAGAA TATGGGAATT AATACAAGAA TGGTTCGATG CACGAATTAA GAGAAAGCCT 
CTTAATATAC GGAATTGTTA TTTCCCAATG TTTGTATCAC AAGGAGCATT AGAAAGAGAA 
AAAGAACATA TTGCAGATTT TGCACCAGAG GTAGCTTGGG TTACCAAATC TGGTGAAACG 
GATTTAGCTG AACCTATTGC TATAAGACCA ACTAGTGAGA CTGGGATGTA TCCAGCTTTT 
GCGAAATGGA TTCAGTCGTA CAGAGACTTG CCATTGAAAC TCAATCAATG GAACAGCGTT 
GTGAGGTGGG AATTCAAACA CCCTCAACCC TTCTTGAGAA CTAGAGAGTT TTTGTGGCAA 
GAGGGTCATA ATGCGTATGC AACTAGAGAA GAAGCAGAAC GTGATGTATT AATAACATTA 
GGTTATTATG AGTATGTCTA CAAAAATATG CTAGCAGTAC CAGTGATAAA AGGTAAAAAA 
ACTGAAAAAG AAAAGTTTGC TGGAGGAATA TTTACTACTA CTTGTGAAGC ATATGTAGCA 
TCTAGTGGTC GCGCAATTCA AGGTGCCACC TCACATTATC TGGGTACCAA TTTTGCTAAA 
ATGTTTGATG TTCATTTTGA ACATCCAGAC ACACACGAGA AATCATTTGT TCATCAAATT 
TCATTTGGAA TGACAACTAG AACTATTGGT GTTATGGTAA TGGTTCATGG TGATGACCGT 
GGGCTGGTTT TACCTCCACG CATAGCATCT ATTCAAGTAA TTATTGTACC ATGTGGTGTA 
GGCGTTAGCA CAGCAAACGA AGTCAAAGAT AATTTGATTA GAACTTGTTT AGATGTATGT 
AACAGATTAA AAGGAGATAG TCTGATAGAA ACTGATGATG AAAATGACAA TAGTGTTATC 
CAACATCGTA ATTCTTCCTT AATGATAAGA GCAGATACCG ACTTGCGAGA TAATTATTCA 
CCTGGATGGA AGTTCAACCA CTGGGAGTTG AAGGGGGTTC CGTTGAGGTT AGAAATTGGA 
CCAAAAGATT TGGATCGAAA CCAAATGGTA GCTGTGCGGC GAGATAATGG AGATAAACTA 
ACTATTCCCT TAGAGGGACG TCCAAATGAT GGTGGAAACG ACAAGATGGT TGTAAGAATA 
ACCGAGCTGT TGAATCAAAT ACAACAAGAT ATGTACAATA AAGCAGAACA AGAAATGAAA 
GAAAATGTTG TGATATGCCG TAAATGGTCA GAGTGTGCTG CGCAGTTAGC AGCAAAACAT 
TTATTATTGA TACCGTTTTG TGGACGTCCT ATTTGCGAGG ATAACATCAA ACGGGATACC 
ACATTGGACG ATGGTACTGC GGATGTTGGA GCTTCGACAA TGGGAGCAAA GTCGTTATGT 
GTGCCATTTG AACAGCCCAA GGGAGCAGAT ATATTGAAAT CCAATGACCA CTGTATACAT 
CCAGAATGTG GGCAGCCAGC TGGGTCTTTC ACTCTATTTG GCCGCAGTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100165283 ( XM_029485418.1 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_029341278.1 Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X2, mRNA.

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CloneID	OAd29969
Clone ID Related Accession (Same CDS sequence)	XM_029485418.1
Accession Version	XM_029485418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	bifunctional glutamate/proline--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TCGGGGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCAGA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCGGGTGGT 
ATTCCCACAC CACAGCTAGT CAGTAATGCC AATTCTGATC AAAGTAAACC TTCAGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAGTAAA 
GCTTCTAAAG CAGACATTGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAGGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATCGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTAA TCAAAGTAAA 
CCTTTAGTAG ATTCTATAGT TGTGAAAATT AATGAACAAG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGGGTT GATTTTCCTG CAAGTGGAAT TCCCACACCA 
CAACAAGCAG ATAAATGTAA CTCCGAGTTA AGTAAACCTT CTTCTGCTGA TGCAGTAGTA 
CAGCATATAA ATGAACAAGG TGAAAAAACA ATGTTGACGA AAAACAGTAA TACTTCCAAA 
GATAAAAAAT CGAAGAAAAA AGAGAATCTT GCTGTGGCCA CTACTAAAAC ATCTGATAGT 
ACAAACACCA AAAAAGTAAC AAAATTAGGG TTGGAAGTAT TAAAGGTATC CGAAAATCTT 
GGAGAATGGT ATTCTCAGGT ATTGGTTAAA GGCGAAATGA TTGAATATTA TGATGTAAGT 
GGATGTTACG TGCTCAGGCC TTGGGCTTAC AGAATATGGG AATTAATACA AGAATGGTTC 
GATGCACGAA TTAAGAGAAA GCCTCTTAAT ATACGGAATT GTTATTTCCC AATGTTTGTA 
TCACAAGGAG CATTAGAAAG AGAAAAAGAA CATATTGCAG ATTTTGCACC AGAGGTAGCT 
TGGGTTACCA AATCTGGTGA AACGGATTTA GCTGAACCTA TTGCTATAAG ACCAACTAGT 
GAGACTGGGA TGTATCCAGC TTTTGCGAAA TGGATTCAGT CGTACAGAGA CTTGCCATTG 
AAACTCAATC AATGGAACAG CGTTGTGAGG TGGGAATTCA AACACCCTCA ACCCTTCTTG 
AGAACTAGAG AGTTTTTGTG GCAAGAGGGT CATAATGCGT ATGCAACTAG AGAAGAAGCA 
GAACGTGATG TATTAATAAC ATTAGGTTAT TATGAGTATG TCTACAAAAA TATGCTAGCA 
GTACCAGTGA TAAAAGGTAA AAAAACTGAA AAAGAAAAGT TTGCTGGAGG AATATTTACT 
ACTACTTGTG AAGCATATGT AGCATCTAGT GGTCGCGCAA TTCAAGGTGC CACCTCACAT 
TATCTGGGTA CCAATTTTGC TAAAATGTTT GATGTTCATT TTGAACATCC AGACACACAC 
GAGAAATCAT TTGTTCATCA AATTTCATTT GGAATGACAA CTAGAACTAT TGGTGTTATG 
GTAATGGTTC ATGGTGATGA CCGTGGGCTG GTTTTACCTC CACGCATAGC ATCTATTCAA 
GTAATTATTG TACCATGTGG TGTAGGCGTT AGCACAGCAA ACGAAGTCAA AGATAATTTG 
ATTAGAACTT GTTTAGATGT ATGTAACAGA TTAAAAGGAG ATAGTCTGAT AGAAACTGAT 
GATGAAAATG ACAATAGTGT TATCCAACAT CGTAATTCTT CCTTAATGAT AAGAGCAGAT 
ACCGACTTGC GAGATAATTA TTCACCTGGA TGGAAGTTCA ACCACTGGGA GTTGAAGGGG 
GTTCCGTTGA GGTTAGAAAT TGGACCAAAA GATTTGGATC GAAACCAAAT GGTAGCTGTG 
CGGCGAGATA ATGGAGATAA ACTAACTATT CCCTTAGAGG GACGTCCAAA TGATGGTGGA 
AACGACAAGA TGGTTGTAAG AATAACCGAG CTGTTGAATC AAATACAACA AGATATGTAC 
AATAAAGCAG AACAAGAAAT GAAAGAAAAT GTTGTGATAT GCCGTAAATG GTCAGAGTGT 
GCTGCGCAGT TAGCAGCAAA ACATTTATTA TTGATACCGT TTTGTGGACG TCCTATTTGC 
GAGGATAACA TCAAACGGGA TACCACATTG GACGATGGTA CTGCGGATGT TGGAGCTTCG 
ACAATGGGAG CAAAGTCGTT ATGTGTGCCA TTTGAACAGC CCAAGGGAGC AGATATATTG 
AAATCCAATG ACCACTGTAT ACATCCAGAA TGTGGGCAGC CAGCTGGGTC TTTCACTCTA 
TTTGGCCGCA GTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029341278.1
CDS	218..4975
Translation	MSNAPEKDLTSLEQQLATPDLQSTNAKKQGSTSVLKSKKTETKKNEKAPKLQKSVEDNSKQQGKFVELPGAEMGKVVVRFPPEASGYLHIGHAKAALLNQHYQLAFNGRLIMRFDDTNPAKEKEDFEKVILEDVDMLKIKPDIFTYTSDHFEKMLDICENGMLKEGLAYVDDTDGDKIRLEREQRIDSINRNNSIEKNLELWKQMVLGTEIGLKCCVRAKIDMQSANGCLRDPTIYRCKPEHHPRTGDKYKVYPTYDFACPIVDSFEGVTHCLRTTEYTDRDAQYYWFCDALKLGTRSNETRPYVWSYSRLNMTNTVLSKRKLTWFVEQGHVDGWDDPRMPTVRGVFRRGMQVEALKQFIVAQGSSRTVTAMQWDKIWSLNRSIVDSIAPRHTALLLSNSNKENVVLSYPPVEVVVENVEKPYKIDNVPKHPKNSQLGLKNCVWAAPKILIDYSDAELLEAGRRATFINWGNLLINRIERNQNNNKIERIHAVSDLENTDFKSTVKVTWLPKTDYSESEEVLPILVPVVCVYFDHLISKAVLAKDDDFKQYLNTENSRWELQMIGDSELKDLKKGDVIQLQRRGYFICDSTYQPYSIHTGKETPLVLFNIPDGHTKSATPITPVPLPITTSNIKKNTNKDIDTVISQINDQANKINVLTADLEEAGKVLHALKAEYKACTGIDFPATGISTPQTVSNSVSGQSNPSADAIVLKINEQGDIVRSMKNNKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSDQSKPSVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPAGGIPTPQLVSNANSNQSKPLVDSIVVKINEQGDKVRSMKNSKASKADIGEAVKVLLALKAEYKSIAGVDFPASGIPTPQQADKCNSELSKPSSADAVVQHINEQGEKTMLTKNSNTSKDKKSKKKENLAVATTKTSDSTNTKKVTKLGLEVLKVSENLGEWYSQVLVKGEMIEYYDVSGCYVLRPWAYRIWELIQEWFDARIKRKPLNIRNCYFPMFVSQGALEREKEHIADFAPEVAWVTKSGETDLAEPIAIRPTSETGMYPAFAKWIQSYRDLPLKLNQWNSVVRWEFKHPQPFLRTREFLWQEGHNAYATREEAERDVLITLGYYEYVYKNMLAVPVIKGKKTEKEKFAGGIFTTTCEAYVASSGRAIQGATSHYLGTNFAKMFDVHFEHPDTHEKSFVHQISFGMTTRTIGVMVMVHGDDRGLVLPPRIASIQVIIVPCGVGVSTANEVKDNLIRTCLDVCNRLKGDSLIETDDENDNSVIQHRNSSLMIRADTDLRDNYSPGWKFNHWELKGVPLRLEIGPKDLDRNQMVAVRRDNGDKLTIPLEGRPNDGGNDKMVVRITELLNQIQQDMYNKAEQEMKENVVICRKWSECAAQLAAKHLLLIPFCGRPICEDNIKRDTTLDDGTADVGASTMGAKSLCVPFEQPKGADILKSNDHCIHPECGQPAGSFTLFGRSY

Target ORF information:

RefSeq Version	XM_029485418.1
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum bifunctional glutamate/proline--tRNA ligase (LOC100165283), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029485418.1

ORF Insert Sequence:

ATGAGTAACG CTCCTGAAAA AGATCTGACC AGTCTGGAGC AGCAATTGGC TACTCCAGAC 
TTGCAAAGTA CAAATGCAAA AAAACAAGGT TCTACTTCTG TCCTCAAAAG TAAGAAAACG 
GAAACTAAAA AAAATGAAAA AGCCCCAAAA CTGCAAAAGT CTGTTGAAGA CAATAGTAAA 
CAACAAGGAA AGTTTGTGGA ATTACCGGGA GCTGAAATGG GAAAAGTTGT TGTTAGATTT 
CCTCCAGAAG CAAGCGGATA TTTGCATATT GGGCATGCAA AAGCTGCTCT ATTAAATCAA 
CACTATCAGT TGGCTTTTAA TGGCCGTCTT ATAATGCGTT TTGATGATAC AAATCCAGCA 
AAGGAGAAGG AAGATTTTGA GAAGGTTATA TTAGAAGACG TTGATATGTT AAAAATTAAA 
CCCGATATTT TTACATATAC TTCTGATCAT TTTGAGAAAA TGTTGGATAT ATGTGAGAAT 
GGAATGTTGA AAGAAGGCTT AGCTTATGTG GATGACACGG ATGGAGATAA AATACGTTTG 
GAACGCGAGC AAAGAATAGA TTCAATAAAT CGAAACAACT CAATTGAGAA GAACCTAGAA 
CTGTGGAAAC AAATGGTTTT AGGTACAGAA ATCGGTCTTA AGTGTTGTGT TCGGGCTAAA 
ATTGATATGC AATCTGCAAA TGGATGTTTG CGAGATCCAA CAATATACCG TTGCAAGCCT 
GAACACCATC CTAGAACTGG TGATAAGTAT AAGGTATATC CAACTTATGA CTTTGCTTGT 
CCTATTGTGG ATAGTTTTGA AGGTGTTACT CATTGTTTAC GTACCACAGA GTATACTGAT 
CGTGATGCTC AATATTATTG GTTTTGTGAT GCATTAAAGC TTGGTACTCG CAGCAATGAA 
ACCAGACCTT ATGTGTGGTC GTATTCAAGA TTAAATATGA CTAATACTGT TCTGTCGAAA 
AGAAAGTTAA CTTGGTTTGT GGAACAAGGC CACGTAGATG GATGGGATGA TCCACGAATG 
CCGACTGTCC GTGGTGTCTT CAGGAGAGGA ATGCAGGTGG AAGCATTGAA ACAATTCATA 
GTGGCCCAAG GATCTTCCAG AACTGTTACA GCTATGCAAT GGGATAAAAT TTGGTCATTG 
AATCGCAGTA TCGTTGATTC GATAGCACCA AGGCATACTG CTTTGTTGTT ATCCAATTCA 
AATAAAGAAA ATGTAGTATT ATCTTATCCA CCAGTTGAAG TTGTTGTAGA GAATGTTGAA 
AAACCATATA AAATTGACAA TGTCCCTAAA CATCCTAAAA ATTCTCAATT AGGACTAAAG 
AATTGTGTCT GGGCCGCACC TAAAATTCTT ATAGATTATA GTGATGCAGA GTTATTAGAA 
GCTGGACGAC GAGCAACATT TATTAACTGG GGAAATCTCC TGATAAACCG TATAGAAAGG 
AACCAAAATA ATAATAAAAT AGAGAGAATT CATGCAGTGT CTGATCTTGA AAACACAGAT 
TTTAAATCTA CAGTCAAAGT TACGTGGTTA CCAAAAACAG ATTATTCAGA ATCTGAAGAA 
GTTTTGCCTA TTCTGGTACC AGTTGTTTGT GTTTATTTTG ATCACTTAAT TAGTAAAGCA 
GTATTAGCCA AAGATGATGA TTTCAAGCAA TACTTAAATA CAGAAAATAG TCGTTGGGAA 
TTACAAATGA TAGGTGATTC AGAGTTGAAA GATTTAAAGA AGGGAGATGT GATTCAGTTA 
CAACGTAGAG GTTATTTTAT ATGTGATTCA ACGTACCAGC CATACAGCAT TCACACGGGA 
AAAGAAACGC CACTAGTGTT GTTTAACATA CCTGATGGTC ACACAAAATC AGCAACTCCA 
ATAACACCCG TTCCTTTACC AATCACCACA AGTAATATTA AGAAAAATAC AAACAAGGAC 
ATTGACACTG TAATCAGCCA GATAAATGAT CAAGCTAATA AAATAAACGT CTTGACGGCT 
GACTTAGAAG AAGCTGGAAA AGTCTTACAT GCATTAAAAG CAGAATATAA GGCTTGCACA 
GGGATTGACT TTCCTGCTAC AGGAATTTCC ACACCACAGA CAGTTAGTAA CTCTGTTTCT 
GGACAAAGTA ATCCATCTGC AGATGCAATA GTCTTAAAAA TTAATGAACA AGGAGACATA 
GTACGATCAA TGAAAAATAA TAAAGCTTCT AAGGCAGACA TCGGGGAAGC TGTCAAAGTC 
TTACTTGCTT TGAAAGCAGA GTATAAAAGT ATTGCTGGAG TTGATTTTCC TGCGGGTGGT 
ATTCCCACAC CACAGCTAGT CAGTAATGCC AATTCTGATC AAAGTAAACC TTCAGTAGAT 
TCTATAGTTG TGAAAATTAA TGAACAAGGA GATAAAGTGC GATCAATGAA AAACAGTAAA 
GCTTCTAAAG CAGACATTGG GGAAGCTGTC AAAGTCTTAC TTGCTTTGAA AGCAGAGTAT 
AAAAGTATTG CTGGAGTTGA TTTTCCTGCG GGTGGTATTC CCACACCACA GCTAGTCAGT 
AATGCCAATT CTGATCAAAG TAAACCTTCA GTAGATTCTA TAGTTGTGAA AATTAATGAA 
CAGGGAGATA AAGTGCGATC AATGAAAAAC AGTAAAGCTT CTAAAGCAGA CATCGGGGAA 
GCTGTCAAAG TCTTACTTGC TTTGAAAGCA GAGTATAAAA GTATTGCTGG AGTTGATTTT 
CCTGCGGGTG GTATTCCCAC ACCACAGCTA GTCAGTAATG CCAATTCTAA TCAAAGTAAA 
CCTTTAGTAG ATTCTATAGT TGTGAAAATT AATGAACAAG GAGATAAAGT GCGATCAATG 
AAAAACAGTA AAGCTTCTAA AGCAGACATC GGGGAAGCTG TCAAAGTCTT ACTTGCTTTG 
AAAGCAGAGT ATAAAAGTAT TGCTGGGGTT GATTTTCCTG CAAGTGGAAT TCCCACACCA 
CAACAAGCAG ATAAATGTAA CTCCGAGTTA AGTAAACCTT CTTCTGCTGA TGCAGTAGTA 
CAGCATATAA ATGAACAAGG TGAAAAAACA ATGTTGACGA AAAACAGTAA TACTTCCAAA 
GATAAAAAAT CGAAGAAAAA AGAGAATCTT GCTGTGGCCA CTACTAAAAC ATCTGATAGT 
ACAAACACCA AAAAAGTAAC AAAATTAGGG TTGGAAGTAT TAAAGGTATC CGAAAATCTT 
GGAGAATGGT ATTCTCAGGT ATTGGTTAAA GGCGAAATGA TTGAATATTA TGATGTAAGT 
GGATGTTACG TGCTCAGGCC TTGGGCTTAC AGAATATGGG AATTAATACA AGAATGGTTC 
GATGCACGAA TTAAGAGAAA GCCTCTTAAT ATACGGAATT GTTATTTCCC AATGTTTGTA 
TCACAAGGAG CATTAGAAAG AGAAAAAGAA CATATTGCAG ATTTTGCACC AGAGGTAGCT 
TGGGTTACCA AATCTGGTGA AACGGATTTA GCTGAACCTA TTGCTATAAG ACCAACTAGT 
GAGACTGGGA TGTATCCAGC TTTTGCGAAA TGGATTCAGT CGTACAGAGA CTTGCCATTG 
AAACTCAATC AATGGAACAG CGTTGTGAGG TGGGAATTCA AACACCCTCA ACCCTTCTTG 
AGAACTAGAG AGTTTTTGTG GCAAGAGGGT CATAATGCGT ATGCAACTAG AGAAGAAGCA 
GAACGTGATG TATTAATAAC ATTAGGTTAT TATGAGTATG TCTACAAAAA TATGCTAGCA 
GTACCAGTGA TAAAAGGTAA AAAAACTGAA AAAGAAAAGT TTGCTGGAGG AATATTTACT 
ACTACTTGTG AAGCATATGT AGCATCTAGT GGTCGCGCAA TTCAAGGTGC CACCTCACAT 
TATCTGGGTA CCAATTTTGC TAAAATGTTT GATGTTCATT TTGAACATCC AGACACACAC 
GAGAAATCAT TTGTTCATCA AATTTCATTT GGAATGACAA CTAGAACTAT TGGTGTTATG 
GTAATGGTTC ATGGTGATGA CCGTGGGCTG GTTTTACCTC CACGCATAGC ATCTATTCAA 
GTAATTATTG TACCATGTGG TGTAGGCGTT AGCACAGCAA ACGAAGTCAA AGATAATTTG 
ATTAGAACTT GTTTAGATGT ATGTAACAGA TTAAAAGGAG ATAGTCTGAT AGAAACTGAT 
GATGAAAATG ACAATAGTGT TATCCAACAT CGTAATTCTT CCTTAATGAT AAGAGCAGAT 
ACCGACTTGC GAGATAATTA TTCACCTGGA TGGAAGTTCA ACCACTGGGA GTTGAAGGGG 
GTTCCGTTGA GGTTAGAAAT TGGACCAAAA GATTTGGATC GAAACCAAAT GGTAGCTGTG 
CGGCGAGATA ATGGAGATAA ACTAACTATT CCCTTAGAGG GACGTCCAAA TGATGGTGGA 
AACGACAAGA TGGTTGTAAG AATAACCGAG CTGTTGAATC AAATACAACA AGATATGTAC 
AATAAAGCAG AACAAGAAAT GAAAGAAAAT GTTGTGATAT GCCGTAAATG GTCAGAGTGT 
GCTGCGCAGT TAGCAGCAAA ACATTTATTA TTGATACCGT TTTGTGGACG TCCTATTTGC 
GAGGATAACA TCAAACGGGA TACCACATTG GACGATGGTA CTGCGGATGT TGGAGCTTCG 
ACAATGGGAG CAAAGTCGTT ATGTGTGCCA TTTGAACAGC CCAAGGGAGC AGATATATTG 
AAATCCAATG ACCACTGTAT ACATCCAGAA TGTGGGCAGC CAGCTGGGTC TTTCACTCTA 
TTTGGCCGCA GTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100165283 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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