AAEL_AAEL010924 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL010924
Entrez Gene ID	5574121
Full Name	calcium-activated chloride channel regulator 2
Synonyms	AAEL010924
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021837259.1	XP_021692951.1	calcium-activated chloride channel regulator 2
XM_001661141.1	XP_001661191.1	AAEL010924-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL010924 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL010924 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe09279	XM_001661141.1 Latest version!	Aedes aegypti AAEL010924-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe116342	XM_021837259.1 Latest version!	Aedes aegypti calcium-activated chloride channel regulator 2 (LOC5574121), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL010924 ( XM_001661141.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001661191.1 Aedes aegypti AAEL010924-RA partial mRNA.

Price & Availability↑

CloneID	OAe09279
Clone ID Related Accession (Same CDS sequence)	XM_001661141.1
Accession Version	XM_001661141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL010924-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811265). COMPLETENESS: incomplete on the 5' end.

ATGCTAACAT CGGCTTCGCA ATACCTTTTC TCAGCGCTGG ACGGCCGGGT CTACTTTGGT 
GATATAACGG TAATTCTACC GCCCCATTGG CCCAACTCTT GCATTCCGTA CAACCAAACG 
AGAACTTCTG CCAGCGGAGA ACGTAGTGAC GTAACTATCA AAACCCACTC GAAAACCGAA 
TCGCCCATTT GGACAAAGCA ATACGCAGGC TGCGGAGAAC CCGGGGAACA AATCTTCATC 
GAGTCGGACA TTCTCGGCAG GGATACCATC GGGCGGGAGT TCGTTCGAGA GTGGGCCAAG 
TACCGATACG GCGTTTTTGA CGAAATCGGT TTCACCAAAG ATCCCGTTTA TCCACGGTGC 
TACATCAATG ACGATCACGA ACTCAAGCTT ACCGGATGTT CCGACGCTCC CGTTCACGAT 
CGCGGTCTTT GTGGCAATCC CTCCTTATAC AACATTACAG ACATCATAGA TCGCAACGCC 
CGCTCCAGTA TCATGTTCGC TGCAGAATCC CCCAGCGTAA CCATGTTCTG CGATGAGGGA 
ACCCACAACC GCTACGCCCC CACCAAACAT AACCAACTCT GCGATCGCCG TAGCACCTTG 
GACGTCATTC TCAAACATCC AGACTTCATC ATGGAAAACA AGATCAATGT AAACCCATCG 
GCAATCGTTA ACACCGCTCC CAAATTTAGC TACAAAACCC GAAAATCCAC GCGCTACGTC 
ATCATTATCG ATGAAACCCT AGACATGCAG CTGCGTGAGT CCTGGAGCTT CCTGCGCTCT 
GCCATCCGAA AATGGGTCGT CTACGACCTC CCGGCCAACA CCGAAGTCGG AATGGTTCTC 
GCCAACGATA CTGCCACAGA AAAAATGCTC CAAATCTCCT CCCTCTACAT CCAGGAAAAC 
AAAGACCTTG TAGCCTCCTT CATACCGTAC TCCCCAAGTG ACTCCCGTCA GCCCGCTTGT 
CTCACCTGCG CAGTTACCGA TGCCATCCAC ATGCTCAACG AGCGCACCCG CATGAACGGC 
CCCGCCAATT CCATAATCCT GATAATCGCC CCTGGCATGG AGTACTCCAT CGACCACACC 
GCTCTGTCAA ACTCTGCCCG ATCCTACCAT ATCCGCATCG CCACCATCAA CTACCCAAAT 
GTCATCCGTC GCCAACCACT TGACGCACTC GCTCTCTCCA CCGGTGGAAC CGCTTACTCC 
GTCTTCGAAA GCAAGTACAA CCTCGAAAAA AGCTACATCA GCACCTACTT CGAGCTATCC 
AACACTCTGT TCAACATCGG CAAGCAGTAC TACGAAGGCA ACCGGAATGA CCTCCCGATC 
GAAATCTACC GCAAGGAGCT TGTCGATTCG GACGACAACG ACGCCGCCTC AGCGCACCAT 
ATGCTGTCGA AGCGGACCAG CCGCACCGCC ACCGGAAGCT TCACGTTAGA CTCGTTCATG 
GGACCTCCGG CGTACTTTTT CGTGTACGTG CACAACACGG AGAACCCGCT GATTGCGAGC 
ATGAAGCTGA CCAGTCCGAG CGGCATCGTT TACTCATCTA GAAGTGACGC TAGATCGTTG 
GTTAAGCAAC TGATGGTGTC CGCTACTCTG AACGAAACCG GAAGTTGGAC GTATAAGATT 
GATCGGTTCA ATGGAAATCC TCAGTCTCAC TACGTTCAGG TTATTGCCAC GCCACGGTCC 
AAGTACGCTC CGACTATCCA GGCCAGATCG TGGATCAGAC AGCGCAGTGG TAGCCCTCCG 
GTAATCTACG CTGAAGTAAA GAAAGGTGAT CTACCGATCA TTGCGGCGTT GGTTGAGGTT 
ACTGTGACGC GTCCGGATGG AATCTGCCCG ACTGGCAGTG GAAACGAGTG CCGTCAGAAA 
ATTAGGTTGT TGGACTCGGG TTCGGGAGAT CCAGATGTTA CTCGAAACGA TGGGATTTAT 
TCTCGTTATT TCAATGCTGA TGACTTTGGG GGCGCGGGAG CTTATCAATT TGAAGTCACC 
ATTACGGATA ATGAGAACAT GGCTTATACT AGGTCGGAGA GCGTGTTGAA GGGGGCTCCT 
CCTCGATGTT GTGGAAGTGC TATTAGAACG ACTCTCATGG CATCCATGGT ATCATTCCAA 
AGAACGCTTC AACCGATGAC AGTATTTTTG TCACAGGATT TGTTGGACTC AATCGGTCAC 
CGAACCATGG GAAAAATCAG TGATCTTACG GTAGATGTCG AAGGAATGAG GGTACGACTG 
AATTGGACCA CTCCAGATAT GGGAGGCCGT GGTATTGCTC GGTATGAGAT TAAGTATGCT 
ACTTCAATTC GGGATATCGT TGACTTTTAT GACACAGCAG CATTTCTATG GCATCACGAT 
ACACCGTTGA TAAATTCGAT TGGTGAGGAG AGCTCTTTTA CTATGAACCT CTCACATGAA 
CCGCATCTGA TTGGTCAGGT TCTGTATTTC TCTGTGAGGG CGCATGCTCT AGGAGTCATG 
GGAAGCTCAG TTTCAAACTA CGTTCGTGTA TTCGTACCCA AACCAAAGCG AGCAACTTCA 
CTATCGCCAT CAACGTCCAT TGGAACCGAA AGTTATGGGG AAGTTTGGTC ATCCTACGAT 
GAGATCCGTG AGGGTCATAA CCGACGTGTG AACATTCATC AGCGTATTGC CGATACGATT 
AGTCTAGAGG TCGAAGTCCT TCTGGCGATC ATCGCTGGAA CTATATTCTT GATCATCATC 
ATTCTTGTTT GTTGCTACTA CTACTTTCTC AAAAAACGCA ACATCACTCA AGAGGATGAT 
CAGAAGCGAC CGATCAAGCC CTTTCCAACG GAAACCAAGA CATCACTCGG ACACAGCGTC 
TTTGTAACTC CGGGAAACGT AACTTCCTCA TCATCTAACG CCACTAGTTC AACGTCCGCA 
TCTCCAGACT ATGTTCAGCA AACCAACGTA TCCACCAACA TTCCAACCAT CTACAACTCG 
CGTACGCTCC CAATCGAGCA AAAGCTCATC GACGAACTCA AACAGCAACA GCAGATGGCC 
GAGACGCAGT CCTACATCAT CACTCAAGCC GACAACATGC AATACAACAC AACACTAACC 
CGAGGTTTCA AACCAGCTCC GACGATCACT GACAACCGAA CCCTTAGCCC GTTCGAGTCA 
TGGTCTGCCT CGCAGCTACT CCAAGAACAC GAACAGCATC AACGCCGAAG AAGTCCCAGC 
TTGCTGGACA ACTATCTGGA CAACGATGTG CAGCAGTTTT ATGCGAGTCA ACACGATCAG 
ATGTCTTTGC TGAACACGGG AGGAGTTGGT GGTCACACTA TGGGTCACAA CCCCCCGGTG 
CCTCCACCTC CGATATACTC CACGGCAAAT CCTCCGTATT TCTACGAACA GCATCAACAG 
CCACCACCGC AGCAGCAGCA CCATCATCTG CAACAACAGC AACAGCAACA ATACGGGGGT 
CACGACGGGT CGATGAACCC CATGACGACC CCGCTCCACG TCACCATAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001661191.1
CDS	1..3474
Translation	MLTSASQYLFSALDGRVYFGDITVILPPHWPNSCIPYNQTRTSASGERSDVTIKTHSKTESPIWTKQYAGCGEPGEQIFIESDILGRDTIGREFVREWAKYRYGVFDEIGFTKDPVYPRCYINDDHELKLTGCSDAPVHDRGLCGNPSLYNITDIIDRNARSSIMFAAESPSVTMFCDEGTHNRYAPTKHNQLCDRRSTLDVILKHPDFIMENKINVNPSAIVNTAPKFSYKTRKSTRYVIIIDETLDMQLRESWSFLRSAIRKWVVYDLPANTEVGMVLANDTATEKMLQISSLYIQENKDLVASFIPYSPSDSRQPACLTCAVTDAIHMLNERTRMNGPANSIILIIAPGMEYSIDHTALSNSARSYHIRIATINYPNVIRRQPLDALALSTGGTAYSVFESKYNLEKSYISTYFELSNTLFNIGKQYYEGNRNDLPIEIYRKELVDSDDNDAASAHHMLSKRTSRTATGSFTLDSFMGPPAYFFVYVHNTENPLIASMKLTSPSGIVYSSRSDARSLVKQLMVSATLNETGSWTYKIDRFNGNPQSHYVQVIATPRSKYAPTIQARSWIRQRSGSPPVIYAEVKKGDLPIIAALVEVTVTRPDGICPTGSGNECRQKIRLLDSGSGDPDVTRNDGIYSRYFNADDFGGAGAYQFEVTITDNENMAYTRSESVLKGAPPRCCGSAIRTTLMASMVSFQRTLQPMTVFLSQDLLDSIGHRTMGKISDLTVDVEGMRVRLNWTTPDMGGRGIARYEIKYATSIRDIVDFYDTAAFLWHHDTPLINSIGEESSFTMNLSHEPHLIGQVLYFSVRAHALGVMGSSVSNYVRVFVPKPKRATSLSPSTSIGTESYGEVWSSYDEIREGHNRRVNIHQRIADTISLEVEVLLAIIAGTIFLIIIILVCCYYYFLKKRNITQEDDQKRPIKPFPTETKTSLGHSVFVTPGNVTSSSSNATSSTSASPDYVQQTNVSTNIPTIYNSRTLPIEQKLIDELKQQQQMAETQSYIITQADNMQYNTTLTRGFKPAPTITDNRTLSPFESWSASQLLQEHEQHQRRRSPSLLDNYLDNDVQQFYASQHDQMSLLNTGGVGGHTMGHNPPVPPPPIYSTANPPYFYEQHQQPPPQQQHHHLQQQQQQQYGGHDGSMNPMTTPLHVTIT

Target ORF information:

RefSeq Version	XM_001661141.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL010924-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001661141.1

ORF Insert Sequence:

ATGCTAACAT CGGCTTCGCA ATACCTTTTC TCAGCGCTGG ACGGCCGGGT CTACTTTGGT 
GATATAACGG TAATTCTACC GCCCCATTGG CCCAACTCTT GCATTCCGTA CAACCAAACG 
AGAACTTCTG CCAGCGGAGA ACGTAGTGAC GTAACTATCA AAACCCACTC GAAAACCGAA 
TCGCCCATTT GGACAAAGCA ATACGCAGGC TGCGGAGAAC CCGGGGAACA AATCTTCATC 
GAGTCGGACA TTCTCGGCAG GGATACCATC GGGCGGGAGT TCGTTCGAGA GTGGGCCAAG 
TACCGATACG GCGTTTTTGA CGAAATCGGT TTCACCAAAG ATCCCGTTTA TCCACGGTGC 
TACATCAATG ACGATCACGA ACTCAAGCTT ACCGGATGTT CCGACGCTCC CGTTCACGAT 
CGCGGTCTTT GTGGCAATCC CTCCTTATAC AACATTACAG ACATCATAGA TCGCAACGCC 
CGCTCCAGTA TCATGTTCGC TGCAGAATCC CCCAGCGTAA CCATGTTCTG CGATGAGGGA 
ACCCACAACC GCTACGCCCC CACCAAACAT AACCAACTCT GCGATCGCCG TAGCACCTTG 
GACGTCATTC TCAAACATCC AGACTTCATC ATGGAAAACA AGATCAATGT AAACCCATCG 
GCAATCGTTA ACACCGCTCC CAAATTTAGC TACAAAACCC GAAAATCCAC GCGCTACGTC 
ATCATTATCG ATGAAACCCT AGACATGCAG CTGCGTGAGT CCTGGAGCTT CCTGCGCTCT 
GCCATCCGAA AATGGGTCGT CTACGACCTC CCGGCCAACA CCGAAGTCGG AATGGTTCTC 
GCCAACGATA CTGCCACAGA AAAAATGCTC CAAATCTCCT CCCTCTACAT CCAGGAAAAC 
AAAGACCTTG TAGCCTCCTT CATACCGTAC TCCCCAAGTG ACTCCCGTCA GCCCGCTTGT 
CTCACCTGCG CAGTTACCGA TGCCATCCAC ATGCTCAACG AGCGCACCCG CATGAACGGC 
CCCGCCAATT CCATAATCCT GATAATCGCC CCTGGCATGG AGTACTCCAT CGACCACACC 
GCTCTGTCAA ACTCTGCCCG ATCCTACCAT ATCCGCATCG CCACCATCAA CTACCCAAAT 
GTCATCCGTC GCCAACCACT TGACGCACTC GCTCTCTCCA CCGGTGGAAC CGCTTACTCC 
GTCTTCGAAA GCAAGTACAA CCTCGAAAAA AGCTACATCA GCACCTACTT CGAGCTATCC 
AACACTCTGT TCAACATCGG CAAGCAGTAC TACGAAGGCA ACCGGAATGA CCTCCCGATC 
GAAATCTACC GCAAGGAGCT TGTCGATTCG GACGACAACG ACGCCGCCTC AGCGCACCAT 
ATGCTGTCGA AGCGGACCAG CCGCACCGCC ACCGGAAGCT TCACGTTAGA CTCGTTCATG 
GGACCTCCGG CGTACTTTTT CGTGTACGTG CACAACACGG AGAACCCGCT GATTGCGAGC 
ATGAAGCTGA CCAGTCCGAG CGGCATCGTT TACTCATCTA GAAGTGACGC TAGATCGTTG 
GTTAAGCAAC TGATGGTGTC CGCTACTCTG AACGAAACCG GAAGTTGGAC GTATAAGATT 
GATCGGTTCA ATGGAAATCC TCAGTCTCAC TACGTTCAGG TTATTGCCAC GCCACGGTCC 
AAGTACGCTC CGACTATCCA GGCCAGATCG TGGATCAGAC AGCGCAGTGG TAGCCCTCCG 
GTAATCTACG CTGAAGTAAA GAAAGGTGAT CTACCGATCA TTGCGGCGTT GGTTGAGGTT 
ACTGTGACGC GTCCGGATGG AATCTGCCCG ACTGGCAGTG GAAACGAGTG CCGTCAGAAA 
ATTAGGTTGT TGGACTCGGG TTCGGGAGAT CCAGATGTTA CTCGAAACGA TGGGATTTAT 
TCTCGTTATT TCAATGCTGA TGACTTTGGG GGCGCGGGAG CTTATCAATT TGAAGTCACC 
ATTACGGATA ATGAGAACAT GGCTTATACT AGGTCGGAGA GCGTGTTGAA GGGGGCTCCT 
CCTCGATGTT GTGGAAGTGC TATTAGAACG ACTCTCATGG CATCCATGGT ATCATTCCAA 
AGAACGCTTC AACCGATGAC AGTATTTTTG TCACAGGATT TGTTGGACTC AATCGGTCAC 
CGAACCATGG GAAAAATCAG TGATCTTACG GTAGATGTCG AAGGAATGAG GGTACGACTG 
AATTGGACCA CTCCAGATAT GGGAGGCCGT GGTATTGCTC GGTATGAGAT TAAGTATGCT 
ACTTCAATTC GGGATATCGT TGACTTTTAT GACACAGCAG CATTTCTATG GCATCACGAT 
ACACCGTTGA TAAATTCGAT TGGTGAGGAG AGCTCTTTTA CTATGAACCT CTCACATGAA 
CCGCATCTGA TTGGTCAGGT TCTGTATTTC TCTGTGAGGG CGCATGCTCT AGGAGTCATG 
GGAAGCTCAG TTTCAAACTA CGTTCGTGTA TTCGTACCCA AACCAAAGCG AGCAACTTCA 
CTATCGCCAT CAACGTCCAT TGGAACCGAA AGTTATGGGG AAGTTTGGTC ATCCTACGAT 
GAGATCCGTG AGGGTCATAA CCGACGTGTG AACATTCATC AGCGTATTGC CGATACGATT 
AGTCTAGAGG TCGAAGTCCT TCTGGCGATC ATCGCTGGAA CTATATTCTT GATCATCATC 
ATTCTTGTTT GTTGCTACTA CTACTTTCTC AAAAAACGCA ACATCACTCA AGAGGATGAT 
CAGAAGCGAC CGATCAAGCC CTTTCCAACG GAAACCAAGA CATCACTCGG ACACAGCGTC 
TTTGTAACTC CGGGAAACGT AACTTCCTCA TCATCTAACG CCACTAGTTC AACGTCCGCA 
TCTCCAGACT ATGTTCAGCA AACCAACGTA TCCACCAACA TTCCAACCAT CTACAACTCG 
CGTACGCTCC CAATCGAGCA AAAGCTCATC GACGAACTCA AACAGCAACA GCAGATGGCC 
GAGACGCAGT CCTACATCAT CACTCAAGCC GACAACATGC AATACAACAC AACACTAACC 
CGAGGTTTCA AACCAGCTCC GACGATCACT GACAACCGAA CCCTTAGCCC GTTCGAGTCA 
TGGTCTGCCT CGCAGCTACT CCAAGAACAC GAACAGCATC AACGCCGAAG AAGTCCCAGC 
TTGCTGGACA ACTATCTGGA CAACGATGTG CAGCAGTTTT ATGCGAGTCA ACACGATCAG 
ATGTCTTTGC TGAACACGGG AGGAGTTGGT GGTCACACTA TGGGTCACAA CCCCCCGGTG 
CCTCCACCTC CGATATACTC CACGGCAAAT CCTCCGTATT TCTACGAACA GCATCAACAG 
CCACCACCGC AGCAGCAGCA CCATCATCTG CAACAACAGC AACAGCAACA ATACGGGGGT 
CACGACGGGT CGATGAACCC CATGACGACC CCGCTCCACG TCACCATAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL010924 ( XM_021837259.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021692951.1 Aedes aegypti calcium-activated chloride channel regulator 2 (LOC5574121), mRNA.

Price & Availability↑

CloneID	OAe116342
Clone ID Related Accession (Same CDS sequence)	XM_021837259.1
Accession Version	XM_021837259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	calcium-activated chloride channel regulator 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA TAAGCGTATT CGTCAAAGTG TTGGCGGTGG CAGCGGCTTT ACTGGTGGTC 
CATGTTGGAC ACATTTCGGC GGCCACCGCT ACCAGTGCGG TCTATGTGGA AAAATCCGCG 
TACAAAAATG TCGTCATCGA GATAAGGGAT AACGTACCGG TGGAAAATTG CCAAACCATC 
CTGCACAACG TGGAGATCAT GCTAACATCG GCTTCGCAAT ACCTTTTCTC AGCGCTGGAC 
GGCCGGGTCT ACTTCGGTGA CATAACGGTA ATTCTACCGC CCCATTGGCC CAACTCTTGC 
ATTCCGTACA ACCAAACGAG AACTTCTGCC AGCGGAGAAC GTAGTGACGT AACTATCAAA 
ACCCACTCGA AAACCGAATC GCCCATTTGG ACAAAGCAAT ACGCAGGCTG CGGAGAACCC 
GGGGAACAAA TCTTCATCGA GTCGGACATT CTCGGCAGGG ATACCATCGG GCGGGAGTTC 
GTTCGAGAGT GGGCCAAGTA CCGATACGGA GTTTTTGACG AAATCGGTTT CACCAAAGAT 
CCCGTTTATC CACGGTGCTA CATCAATGAC GATCACGAAC TCAAGCTTAC CGGATGTTCC 
GACGCTCCCG TTCACGATCG CGGTCTTTGT GGCAATCCCT CCTCGTACAA CATTACAGAC 
ATCATAGATC GCAACGCCCG CTCCAGTATC ATGTTCGCTG CAGAATCCCC CAGCGTAACC 
ATGTTCTGCG ATGAAGGAAC CCACAACCGC TACGCCCCCA CCAAACATAA CCAACTCTGC 
GATCGCCGTA GCACCTTGGA CGTCATTCTT AAACATCCAG ACTTCATCAT GGAAAACAAG 
ATCAATGTAA ACCCATCGGT AATCATTAAC ACCGCCCCCA AATTTAGCTA CAAAACCCGT 
AAATCCACGC GCTACGTTAT CATTATCGAT GAAACCCTTG ACATGCAGCT GCGTGAATCC 
TGGAGCTTCT TGCGGTCAGC CATCCGAAAA TGGGTCGTCT ACGACCTCCC GGCCAATACC 
GAAGTCGGAA TGGTTCTCGC CAACGATACC GCCACAGAAA AGATGCTCCA AATTTCCTCC 
CTCTACATCC AGGAAAACAA AGACCTTGTA GCCTCCTTCA TACCGTACTC CCCAAGTGAC 
TCCCGTCAGC CCGCCTGTCT CACCTGCGCA GTTACCGATG CCATCGACAT GCTCAACGAG 
CGCACCCGCA TGAACGGCCC CGCCAATTCC ATCATCCTGA TAATCGCCCC TGGCATGGAG 
TACTCCATCG ACCACACCGC TCTGTCAAAC TCTGCCCGAT CCTCCCATAT CCGCATCGCC 
ACCATCAACT ACCCAAATGT CATCCGTCGC CAACCACTTG ACGCACTCGC TCTCTCCACC 
GGTGGAACCG CATACTCCGT CTTCGAAAGC AAGTACAACC TCGAAAAAAG CTACATCAGC 
ACCTACTTCG AGCTATCCAA CACTCTGTTC AACATCGGCA AGCAGTACTA CGAAGGCAAC 
CGGAATGACC TCCCGATCGA AATCTACCGC AAGGAGCTTG TCGATTCGGA CGACAACGAC 
GCCGCCTCAG CGCACCATAT GCTGTCGAAG CGGACCAGCC GCACCGCCAC CGGAAGCTTC 
ACGTTAGACT CGTTTATGGG ACCTCCGGCG TACTTTTTCG TGTACGTGCA CAACACGGAG 
AACCCGCTGA TCGCGAGCAT GAAGCTGACC AGTCCGAGCG GCATCGTTTA CTCGTCTAGA 
AGTGACGCTA GATCGTTGGT TAAGCAACTG ATGGTGTCCG CTACGCTGAA CGAAACCGGA 
AGCTGGACGT ACAAGATTGA TCGCTTCAAT GGTAACCCTC AGTCTCACTA CGTTCAGGTT 
ATTGCCACGC CACGTTCCAA GTACGCTCCG ACTATCCAGG CCAGATCGTG GATCAGACAG 
CGCAGTGGTA GCCCTCCGGT CATCTACGCT GAAGTGAAGA AAGGTGATCT ACCGATCATT 
GCGGCGCTGG TTGAAGTTAC TGTGACGCGT CCGGATGGAA TCTGTCCGAC CGGTAGTGGA 
AACGAATGCC GTCAGAAAAT TAGGTTGTTG GACTCGGGTT CGGGAGATCC GGATGTTACT 
CGAAACGATG GAATTTATTC TCGTTATTTC AATGCTGATG ACTTTGGGGG CGCGGGAGCT 
TATCAATTTG AAGTCACCAT TACGGATAAT GAGAACATGG CTTATACTAG GTCGGAGAGC 
GTGTTGAAGG GGGCTCCTCC TCGATGTTGT GGAAGTGCTA TTAGAACGAC TCTCATGGCA 
TCCATGGTAT CATTCCAAAG AAAGCTTCAA CCGATGACAG TATTTTTGTC ACAGGATTTG 
TTGGACTCAA TCGGTCACCG AACCATGGGA AAAATCAGTG ATCTTACGGT AGATGTCGAA 
GGAATGAGGG TACGACTGAA TTGGACCACT CCAGATATGG GAGGCCGTGG TATTGCTCGG 
TATGAGATTA AGTATGCTAC TTCGATTCGA GATATCGTTG ACTTTTATGA CACAGCAGCA 
TTTCTATGGC ATCACGATAC ACCATTGATA AATTCGATTG GTGAGGAGAG CTCTTTTACT 
ATGAACCTCT CACATGAACC GCATCTGATT GGTCAGGTTC TGTATTTCTC TGTGAGGGCG 
CATGCTCTAG GAGTCATGGG AGGCTCAGTT TCAAACTACG TTCGTGTATT CGTACCCAAA 
CCAAAGCGAG CAACTTCACT ATCGCCATCA ACGTCCATTG GAACCGAAAG TTATGGAGAA 
GTTTGGTCAT CCTACGATGA GATCCGTGAG GGTCATAACC GACGTGTGAA CATTCATCAG 
CGTATTGCCG ATACGATTAG TCTAGAGGTC GAAGTCCTCC TGGCGATCAT CGCTGGAACT 
ATATTCTTGA TCATCATCAT TCTTGTTTGC TGCTACTACT ACTTTCTCAA AAAACGCAAC 
ATCACTCAAG AGGATGATCA GAAGCGACCG ATCAAGCCCT TTTCAACGGA AACCAAGACA 
TCACTCGGAC ACAGCGTCTT TGTAACTCCG GGAAACGTAA CTTCCTCATC ATCTAACGCC 
ACTAGTTCAA CGTCCGCATC TCCAGACTAT GTTCAGCAAA CCAACGTATC CACCAACATT 
CCAACCATCT ACAACTCTCG TACGCTCCCA ATCGAGCAAA AGCTCATCGA TGAACTCAAA 
CAGCAACAGC AGATGGCCGA GACGCAGTCC TACATCATCA CTCAAGCCGA CAACATGCAA 
TACAACACAA CACTAACCCG AGGTTTCAAG CCAGCTCCGA CGATCACTGA CAACCGAACC 
CTTAGCCCGT TCGAGTCATG GTCTGCCTCG CAGCTACTCC AAGAACACGA ACAGCATCAA 
CGCCGAAGAA GTCCCAGCTT GCTGGACAAC TATCTGGACA ACGATGTGCA GCAGTTTTAT 
GCGAGTCAAC ACGATCAGAT GTCTTTGCTG AACACGGGAG GAGTTGGTGG TCACACTATG 
GGTCACAACC CCCCGGTGCC TCCACCACCG ATATACTCCA CGGCAAATCC TCCGTATTTC 
TACGAACAGC ATCAACAGCC ACCACCGCAG CAGCAGCACC ATCACCTGCA ACAACAGCAG 
CAGCAACAAT ACGGGGGTCA CGACGGGTCG ATGAACCCCA TGACGACCCC GCTCCACGTC 
ACCATAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021692951.1
CDS	866..4537
Translation	MLPISVFVKVLAVAAALLVVHVGHISAATATSAVYVEKSAYKNVVIEIRDNVPVENCQTILHNVEIMLTSASQYLFSALDGRVYFGDITVILPPHWPNSCIPYNQTRTSASGERSDVTIKTHSKTESPIWTKQYAGCGEPGEQIFIESDILGRDTIGREFVREWAKYRYGVFDEIGFTKDPVYPRCYINDDHELKLTGCSDAPVHDRGLCGNPSSYNITDIIDRNARSSIMFAAESPSVTMFCDEGTHNRYAPTKHNQLCDRRSTLDVILKHPDFIMENKINVNPSVIINTAPKFSYKTRKSTRYVIIIDETLDMQLRESWSFLRSAIRKWVVYDLPANTEVGMVLANDTATEKMLQISSLYIQENKDLVASFIPYSPSDSRQPACLTCAVTDAIDMLNERTRMNGPANSIILIIAPGMEYSIDHTALSNSARSSHIRIATINYPNVIRRQPLDALALSTGGTAYSVFESKYNLEKSYISTYFELSNTLFNIGKQYYEGNRNDLPIEIYRKELVDSDDNDAASAHHMLSKRTSRTATGSFTLDSFMGPPAYFFVYVHNTENPLIASMKLTSPSGIVYSSRSDARSLVKQLMVSATLNETGSWTYKIDRFNGNPQSHYVQVIATPRSKYAPTIQARSWIRQRSGSPPVIYAEVKKGDLPIIAALVEVTVTRPDGICPTGSGNECRQKIRLLDSGSGDPDVTRNDGIYSRYFNADDFGGAGAYQFEVTITDNENMAYTRSESVLKGAPPRCCGSAIRTTLMASMVSFQRKLQPMTVFLSQDLLDSIGHRTMGKISDLTVDVEGMRVRLNWTTPDMGGRGIARYEIKYATSIRDIVDFYDTAAFLWHHDTPLINSIGEESSFTMNLSHEPHLIGQVLYFSVRAHALGVMGGSVSNYVRVFVPKPKRATSLSPSTSIGTESYGEVWSSYDEIREGHNRRVNIHQRIADTISLEVEVLLAIIAGTIFLIIIILVCCYYYFLKKRNITQEDDQKRPIKPFSTETKTSLGHSVFVTPGNVTSSSSNATSSTSASPDYVQQTNVSTNIPTIYNSRTLPIEQKLIDELKQQQQMAETQSYIITQADNMQYNTTLTRGFKPAPTITDNRTLSPFESWSASQLLQEHEQHQRRRSPSLLDNYLDNDVQQFYASQHDQMSLLNTGGVGGHTMGHNPPVPPPPIYSTANPPYFYEQHQQPPPQQQHHHLQQQQQQQYGGHDGSMNPMTTPLHVTIT

Target ORF information:

RefSeq Version	XM_021837259.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti calcium-activated chloride channel regulator 2 (LOC5574121), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021837259.1

ORF Insert Sequence:

ATGTTGCCAA TAAGCGTATT CGTCAAAGTG TTGGCGGTGG CAGCGGCTTT ACTGGTGGTC 
CATGTTGGAC ACATTTCGGC GGCCACCGCT ACCAGTGCGG TCTATGTGGA AAAATCCGCG 
TACAAAAATG TCGTCATCGA GATAAGGGAT AACGTACCGG TGGAAAATTG CCAAACCATC 
CTGCACAACG TGGAGATCAT GCTAACATCG GCTTCGCAAT ACCTTTTCTC AGCGCTGGAC 
GGCCGGGTCT ACTTCGGTGA CATAACGGTA ATTCTACCGC CCCATTGGCC CAACTCTTGC 
ATTCCGTACA ACCAAACGAG AACTTCTGCC AGCGGAGAAC GTAGTGACGT AACTATCAAA 
ACCCACTCGA AAACCGAATC GCCCATTTGG ACAAAGCAAT ACGCAGGCTG CGGAGAACCC 
GGGGAACAAA TCTTCATCGA GTCGGACATT CTCGGCAGGG ATACCATCGG GCGGGAGTTC 
GTTCGAGAGT GGGCCAAGTA CCGATACGGA GTTTTTGACG AAATCGGTTT CACCAAAGAT 
CCCGTTTATC CACGGTGCTA CATCAATGAC GATCACGAAC TCAAGCTTAC CGGATGTTCC 
GACGCTCCCG TTCACGATCG CGGTCTTTGT GGCAATCCCT CCTCGTACAA CATTACAGAC 
ATCATAGATC GCAACGCCCG CTCCAGTATC ATGTTCGCTG CAGAATCCCC CAGCGTAACC 
ATGTTCTGCG ATGAAGGAAC CCACAACCGC TACGCCCCCA CCAAACATAA CCAACTCTGC 
GATCGCCGTA GCACCTTGGA CGTCATTCTT AAACATCCAG ACTTCATCAT GGAAAACAAG 
ATCAATGTAA ACCCATCGGT AATCATTAAC ACCGCCCCCA AATTTAGCTA CAAAACCCGT 
AAATCCACGC GCTACGTTAT CATTATCGAT GAAACCCTTG ACATGCAGCT GCGTGAATCC 
TGGAGCTTCT TGCGGTCAGC CATCCGAAAA TGGGTCGTCT ACGACCTCCC GGCCAATACC 
GAAGTCGGAA TGGTTCTCGC CAACGATACC GCCACAGAAA AGATGCTCCA AATTTCCTCC 
CTCTACATCC AGGAAAACAA AGACCTTGTA GCCTCCTTCA TACCGTACTC CCCAAGTGAC 
TCCCGTCAGC CCGCCTGTCT CACCTGCGCA GTTACCGATG CCATCGACAT GCTCAACGAG 
CGCACCCGCA TGAACGGCCC CGCCAATTCC ATCATCCTGA TAATCGCCCC TGGCATGGAG 
TACTCCATCG ACCACACCGC TCTGTCAAAC TCTGCCCGAT CCTCCCATAT CCGCATCGCC 
ACCATCAACT ACCCAAATGT CATCCGTCGC CAACCACTTG ACGCACTCGC TCTCTCCACC 
GGTGGAACCG CATACTCCGT CTTCGAAAGC AAGTACAACC TCGAAAAAAG CTACATCAGC 
ACCTACTTCG AGCTATCCAA CACTCTGTTC AACATCGGCA AGCAGTACTA CGAAGGCAAC 
CGGAATGACC TCCCGATCGA AATCTACCGC AAGGAGCTTG TCGATTCGGA CGACAACGAC 
GCCGCCTCAG CGCACCATAT GCTGTCGAAG CGGACCAGCC GCACCGCCAC CGGAAGCTTC 
ACGTTAGACT CGTTTATGGG ACCTCCGGCG TACTTTTTCG TGTACGTGCA CAACACGGAG 
AACCCGCTGA TCGCGAGCAT GAAGCTGACC AGTCCGAGCG GCATCGTTTA CTCGTCTAGA 
AGTGACGCTA GATCGTTGGT TAAGCAACTG ATGGTGTCCG CTACGCTGAA CGAAACCGGA 
AGCTGGACGT ACAAGATTGA TCGCTTCAAT GGTAACCCTC AGTCTCACTA CGTTCAGGTT 
ATTGCCACGC CACGTTCCAA GTACGCTCCG ACTATCCAGG CCAGATCGTG GATCAGACAG 
CGCAGTGGTA GCCCTCCGGT CATCTACGCT GAAGTGAAGA AAGGTGATCT ACCGATCATT 
GCGGCGCTGG TTGAAGTTAC TGTGACGCGT CCGGATGGAA TCTGTCCGAC CGGTAGTGGA 
AACGAATGCC GTCAGAAAAT TAGGTTGTTG GACTCGGGTT CGGGAGATCC GGATGTTACT 
CGAAACGATG GAATTTATTC TCGTTATTTC AATGCTGATG ACTTTGGGGG CGCGGGAGCT 
TATCAATTTG AAGTCACCAT TACGGATAAT GAGAACATGG CTTATACTAG GTCGGAGAGC 
GTGTTGAAGG GGGCTCCTCC TCGATGTTGT GGAAGTGCTA TTAGAACGAC TCTCATGGCA 
TCCATGGTAT CATTCCAAAG AAAGCTTCAA CCGATGACAG TATTTTTGTC ACAGGATTTG 
TTGGACTCAA TCGGTCACCG AACCATGGGA AAAATCAGTG ATCTTACGGT AGATGTCGAA 
GGAATGAGGG TACGACTGAA TTGGACCACT CCAGATATGG GAGGCCGTGG TATTGCTCGG 
TATGAGATTA AGTATGCTAC TTCGATTCGA GATATCGTTG ACTTTTATGA CACAGCAGCA 
TTTCTATGGC ATCACGATAC ACCATTGATA AATTCGATTG GTGAGGAGAG CTCTTTTACT 
ATGAACCTCT CACATGAACC GCATCTGATT GGTCAGGTTC TGTATTTCTC TGTGAGGGCG 
CATGCTCTAG GAGTCATGGG AGGCTCAGTT TCAAACTACG TTCGTGTATT CGTACCCAAA 
CCAAAGCGAG CAACTTCACT ATCGCCATCA ACGTCCATTG GAACCGAAAG TTATGGAGAA 
GTTTGGTCAT CCTACGATGA GATCCGTGAG GGTCATAACC GACGTGTGAA CATTCATCAG 
CGTATTGCCG ATACGATTAG TCTAGAGGTC GAAGTCCTCC TGGCGATCAT CGCTGGAACT 
ATATTCTTGA TCATCATCAT TCTTGTTTGC TGCTACTACT ACTTTCTCAA AAAACGCAAC 
ATCACTCAAG AGGATGATCA GAAGCGACCG ATCAAGCCCT TTTCAACGGA AACCAAGACA 
TCACTCGGAC ACAGCGTCTT TGTAACTCCG GGAAACGTAA CTTCCTCATC ATCTAACGCC 
ACTAGTTCAA CGTCCGCATC TCCAGACTAT GTTCAGCAAA CCAACGTATC CACCAACATT 
CCAACCATCT ACAACTCTCG TACGCTCCCA ATCGAGCAAA AGCTCATCGA TGAACTCAAA 
CAGCAACAGC AGATGGCCGA GACGCAGTCC TACATCATCA CTCAAGCCGA CAACATGCAA 
TACAACACAA CACTAACCCG AGGTTTCAAG CCAGCTCCGA CGATCACTGA CAACCGAACC 
CTTAGCCCGT TCGAGTCATG GTCTGCCTCG CAGCTACTCC AAGAACACGA ACAGCATCAA 
CGCCGAAGAA GTCCCAGCTT GCTGGACAAC TATCTGGACA ACGATGTGCA GCAGTTTTAT 
GCGAGTCAAC ACGATCAGAT GTCTTTGCTG AACACGGGAG GAGTTGGTGG TCACACTATG 
GGTCACAACC CCCCGGTGCC TCCACCACCG ATATACTCCA CGGCAAATCC TCCGTATTTC 
TACGAACAGC ATCAACAGCC ACCACCGCAG CAGCAGCACC ATCACCTGCA ACAACAGCAG 
CAGCAACAAT ACGGGGGTCA CGACGGGTCG ATGAACCCCA TGACGACCCC GCTCCACGTC 
ACCATAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL010924 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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