OT05G03050 cDNA ORF clone, Ostreococcus tauri

Gene
Clones
gRNAs

Summary

Gene Symbol	OT05G03050
Entrez Gene ID	9835204
Full Name	Sad1/UNC-like, C-terminal
Synonyms	Ot05g03050
Gene Type	protein-coding
Organism	Ostreococcus tauri

mRNA and Protein(s)

mRNA	Protein	Name
XM_022984286.1	XP_022839032.1	Sad1/UNC-like, C-terminal
XM_003079354.1	XP_003079402.1	unc-84 homolog B-like (ISS)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
Blanc-Mathieu R, Verhelst B, Derelle E, Rombauts S, Bouget FY, Carré I, Château A, Eyre-Walker A, Grimsley N, Moreau H, Piégu B, Rivals E, Schackwitz W, Van de Peer Y, Piganeau G
BMC genomics151103(2014 Dec)

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Derelle E, Ferraz C, Rombauts S, Rouzé P, Worden AZ, Robbens S, Partensky F, Degroeve S, Echeynié S, Cooke R, Saeys Y, Wuyts J, Jabbari K, Bowler C, Panaud O, Piégu B, Ball SG, Ral JP, Bouget FY, Piganeau G, De Baets B, Picard A, Delseny M, Demaille J, Van de Peer Y, Moreau H
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OT05G03050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OT05G03050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd03863	XM_003079354.1 Latest version!	Ostreococcus tauri unc-84 homolog B-like (ISS) (Ot05g03050) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOd75287	XM_022984286.1 Latest version!	Ostreococcus tauri Sad1/UNC-like, C-terminal (OT_ostta05g02920), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OT05G03050 ( XM_003079354.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_003079402.1 Ostreococcus tauri unc-84 homolog B-like (ISS) (Ot05g03050) mRNA, complete cds.

Price & Availability↑

CloneID	OOd03863
Clone ID Related Accession (Same CDS sequence)	XM_003079354.1
Accession Version	XM_003079354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1373472000000
Organism	Ostreococcus tauri
Product	unc-84 homolog B-like (ISS)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014430). COMPLETENESS: incomplete on both ends.

ATGAAGACCA CGGCGGTGCT CAACGCGAAG GTTCCTTTCG CCGTCGCCGC GTCGCTGGTG 
ATCGCGACCG CGGGATGCGC GACGGCGGCG CTGGGATGGC ACGCGCGAGA GACGGCGTTG 
GCGCTCGCGA GCGCGCGGGT GAGCGCGACG AGCGTCGATT TAGGGCCGGC GACGCGAAGG 
ATACGGGTGC TTGAGGAGCA AGTGGAAGCC CTTCAAAGGA ATCGCGAGGA TGGTCGCAAA 
TCGGCGGAGC GAGTGGTGCG CGAGGCCTTG CTTGACGCCC AAGTCAAGGC TCTTGGAACG 
CAAAAGGGCA GCAAGGTTGC CGGTTTGTTC AAGCGCGACG CGGATACGCC TGCGTTGAAG 
AAGGACTTGA AGGCCATCGA GACGACGCTC GCGTCCTTGA CGAAGAGTCA AGGTGCGTTT 
GCGACATCCG CGCAGTTGAA GGAACTCGTC GACGCCGTCG ACGCCTTGCG AAAGTCGTCG 
AGTGGGTACG CTAGCTCGGA TGTCGTTGAG ACTTTGAAGG TGAGTGTGAC GGAGCTGACG 
AATAAGACGA AGTCGGGATC GATGAAGGAA GTCGCCGCGC TCCGGAAGGC GCTCGAGGAG 
TTGGCGTCAA CACAAAAGTC GGTTGACGCA CGAGCCACGT CTGAAGTGAA GAGCTTGCGG 
GATGACATCA CCTCTCTGAA GACGAATATT CAGGAGACGT ACGCCTCGAA GTCGGCGTTG 
CAAACGCTGG ACGTTTCTAG TGAGCTCAAG TCTTTGGAAG CTGCGATCGC GGGCTTATCC 
AAGGATCGTG ACTCATACGC GACGGCGACT CAACTCAAGT TGCTTGACGC CCAAGTCAAG 
GCTCTTGGAA CGCAAAAGGG CAGCAAGGTT GCCGGTTTGT TCAAGCGCGA CGCGGATACG 
GCTACGTTGA AGAAGGACTT GAAGGCCATC GAGACGACGC TCGCGTCCTT GACGAAGAGT 
CAAGGTGCGT TTGCGACATC CGCGCAGTTG AAGGAACTCG TCGACGCCGT CGACGCCTTG 
CGAAAGTCGT CGAGTGGGTA CGCTAGCTCG GATGTCGTTG AGACTTTGAA GGTGAGTGTG 
ACGGAGCTGA CGAATAAGAC GAAGTCGGGA TCGATGAAGG AAGTCGCCGC GCTCCGGAAG 
GCGCTCGAGG AGTTGGCGTC AACACAAAAG TCGGTTGACG CGCGAGCCAC GTCTGAAGTG 
AAGAGCTTGC GGGATGACAT CACCTCTCTG AAGACGAATA TTCAGGAGAC GTACGCCTCG 
AAGTCGGCGT TGCAAACGCT GGACGTTTCT AGTGAGCTCA AGTCTTTGGA AGCTGCGATC 
GCGGGCTTAT CCAAGGATCG TGACTCATAC GCGACGGCGA CTCAACTCAA GTTGCTTGAC 
GCCCAAGTCA AGGCTCTTGG AACGCAAAAG GGCAGCAAGG TTGCCGGTTT GTTCAAGCGC 
GACGCGGATA CGGCTACGTT GAAGAAGGAC TTGAAGGCCA TCGAGACGAC GCTCGTGTCC 
TTGACGAAGA GTCAAGGTGC GTTTGCGACA TCCGCGCAGT TGAAGGAACT CGTCGACGCC 
GTCGACGCCT TGCGGAAGTC GTCGAGTGGG TACGCTAGCT CGGATGTCGT TGAGACTTTG 
AAGGTGAGTG TGACGGAGCT GACGAATAAG ACGAAGTCGG GATCGATGAA GGAAGTCGCC 
GCGCTCCGGA AGGCGCTCGA GGAGTTGGCG TCAACACAAA AGTCGGTTGA CGCGCGAGCC 
ACGTCTGAAG TGAAGAGCTT GCGGGATGAT ATCACCTCTC TGAAGACGAA TATTCAGGAG 
ACGTACGCCT CGAAGTCGGC GTTGCAAACG CTGGACGTTT CTAGTGAGCT CAAGTCTTTG 
GAAGCTGCGA TCGCGGGCTT ATCCAAGGAT CGTGACTCAT ACGCGACGGC GACTCAACTC 
AAGTTGCTTG ACGCCCAAGT CAAGGCTCTT GGAACGCAAA AGGGCAGCAA GGTTGCCGGT 
TTGTTCAAGC GCGACGCGGA TACGGCTACG TTGAAGAAGG ACTTGAAGGC CATTGAGACG 
ACGCTCGTGT CCTTGACGAA GAGTCAAGGT GCGTTTGCGA CATCCGCGCA GTTGAAGGAA 
CTCGTCGACG CCGTCGACGC CTTGCGAAAG TCGTCGAGTG GGTACGCTAG CTCGGATGTC 
GTTGAGACTT TGAAGGTGAG TGTGACGGAG CTGACGGATA AGACGAAGTC GGGATCCGGT 
TCGGCGTCGA AGAACCTTCG TGATTTGGAA CAGCAAGTGA TGGAGCGCAT AGAAGGCTAC 
ATCAACGCCA TTCCGAAGGA CACGTCGATG AAGCTCAAGA AGCACATTGA AAAAGCCGCG 
ACGTTGTGGT TTGCAGATAG GACCGGTCGA CAAGATTTTG CGCTATCCAC CGGTGGCGGA 
CGCGTCGTCG GACACTCGCA ACTTACGCCG TTCGTTGCTC GAGGCGATGG ACCGCTGATG 
TCGGCTGTTT CGTTCCTTCG ATCTGGAGTA CATCCAAAAA GCGATGAGTG GATGCTCACC 
CCCTCGATGG AACAGCCGGG CGATTGTCTT GCACTGCATG GATCTTACGG ATATGTCGAT 
GTTCGTCTGC GTCAACCCGT CAAGGTGGAT GCGGTTACTC TCGAGCACAC CAATAGCCTA 
AACGCGTACG ACATGCACAG CGCTCCACGA GACATACAAA TATTCGGCTG GTACGCCCAT 
GGCAATAACT GCAAGCATAG CAAACCACCA AAGTCATTAA TTGCGATGGG AAATTACACG 
TATCACACGG CAGGGGACAG TGTGCAGTCG TTTGAGGTCA GCGCGCCTCA TACGGTCGAT 
CACGTGCGTC TTATAGTGAA AAACAACCAC GGGCACGCGC GATGGACGTG CATATATCGC 
TTCCGCGTGC ATGGCATACC GTCGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003079402.1
CDS	1..2910
Translation	MKTTAVLNAKVPFAVAASLVIATAGCATAALGWHARETALALASARVSATSVDLGPATRRIRVLEEQVEALQRNREDGRKSAERVVREALLDAQVKALGTQKGSKVAGLFKRDADTPALKKDLKAIETTLASLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTNKTKSGSMKEVAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLASLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTNKTKSGSMKEVAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLVSLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTNKTKSGSMKEVAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLVSLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTDKTKSGSGSASKNLRDLEQQVMERIEGYINAIPKDTSMKLKKHIEKAATLWFADRTGRQDFALSTGGGRVVGHSQLTPFVARGDGPLMSAVSFLRSGVHPKSDEWMLTPSMEQPGDCLALHGSYGYVDVRLRQPVKVDAVTLEHTNSLNAYDMHSAPRDIQIFGWYAHGNNCKHSKPPKSLIAMGNYTYHTAGDSVQSFEVSAPHTVDHVRLIVKNNHGHARWTCIYRFRVHGIPSS

Target ORF information:

RefSeq Version	XM_003079354.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri unc-84 homolog B-like (ISS) (Ot05g03050) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003079354.1

ORF Insert Sequence:

ATGAAGACCA CGGCGGTGCT CAACGCGAAG GTTCCTTTCG CCGTCGCCGC GTCGCTGGTG 
ATCGCGACCG CGGGATGCGC GACGGCGGCG CTGGGATGGC ACGCGCGAGA GACGGCGTTG 
GCGCTCGCGA GCGCGCGGGT GAGCGCGACG AGCGTCGATT TAGGGCCGGC GACGCGAAGG 
ATACGGGTGC TTGAGGAGCA AGTGGAAGCC CTTCAAAGGA ATCGCGAGGA TGGTCGCAAA 
TCGGCGGAGC GAGTGGTGCG CGAGGCCTTG CTTGACGCCC AAGTCAAGGC TCTTGGAACG 
CAAAAGGGCA GCAAGGTTGC CGGTTTGTTC AAGCGCGACG CGGATACGCC TGCGTTGAAG 
AAGGACTTGA AGGCCATCGA GACGACGCTC GCGTCCTTGA CGAAGAGTCA AGGTGCGTTT 
GCGACATCCG CGCAGTTGAA GGAACTCGTC GACGCCGTCG ACGCCTTGCG AAAGTCGTCG 
AGTGGGTACG CTAGCTCGGA TGTCGTTGAG ACTTTGAAGG TGAGTGTGAC GGAGCTGACG 
AATAAGACGA AGTCGGGATC GATGAAGGAA GTCGCCGCGC TCCGGAAGGC GCTCGAGGAG 
TTGGCGTCAA CACAAAAGTC GGTTGACGCA CGAGCCACGT CTGAAGTGAA GAGCTTGCGG 
GATGACATCA CCTCTCTGAA GACGAATATT CAGGAGACGT ACGCCTCGAA GTCGGCGTTG 
CAAACGCTGG ACGTTTCTAG TGAGCTCAAG TCTTTGGAAG CTGCGATCGC GGGCTTATCC 
AAGGATCGTG ACTCATACGC GACGGCGACT CAACTCAAGT TGCTTGACGC CCAAGTCAAG 
GCTCTTGGAA CGCAAAAGGG CAGCAAGGTT GCCGGTTTGT TCAAGCGCGA CGCGGATACG 
GCTACGTTGA AGAAGGACTT GAAGGCCATC GAGACGACGC TCGCGTCCTT GACGAAGAGT 
CAAGGTGCGT TTGCGACATC CGCGCAGTTG AAGGAACTCG TCGACGCCGT CGACGCCTTG 
CGAAAGTCGT CGAGTGGGTA CGCTAGCTCG GATGTCGTTG AGACTTTGAA GGTGAGTGTG 
ACGGAGCTGA CGAATAAGAC GAAGTCGGGA TCGATGAAGG AAGTCGCCGC GCTCCGGAAG 
GCGCTCGAGG AGTTGGCGTC AACACAAAAG TCGGTTGACG CGCGAGCCAC GTCTGAAGTG 
AAGAGCTTGC GGGATGACAT CACCTCTCTG AAGACGAATA TTCAGGAGAC GTACGCCTCG 
AAGTCGGCGT TGCAAACGCT GGACGTTTCT AGTGAGCTCA AGTCTTTGGA AGCTGCGATC 
GCGGGCTTAT CCAAGGATCG TGACTCATAC GCGACGGCGA CTCAACTCAA GTTGCTTGAC 
GCCCAAGTCA AGGCTCTTGG AACGCAAAAG GGCAGCAAGG TTGCCGGTTT GTTCAAGCGC 
GACGCGGATA CGGCTACGTT GAAGAAGGAC TTGAAGGCCA TCGAGACGAC GCTCGTGTCC 
TTGACGAAGA GTCAAGGTGC GTTTGCGACA TCCGCGCAGT TGAAGGAACT CGTCGACGCC 
GTCGACGCCT TGCGGAAGTC GTCGAGTGGG TACGCTAGCT CGGATGTCGT TGAGACTTTG 
AAGGTGAGTG TGACGGAGCT GACGAATAAG ACGAAGTCGG GATCGATGAA GGAAGTCGCC 
GCGCTCCGGA AGGCGCTCGA GGAGTTGGCG TCAACACAAA AGTCGGTTGA CGCGCGAGCC 
ACGTCTGAAG TGAAGAGCTT GCGGGATGAT ATCACCTCTC TGAAGACGAA TATTCAGGAG 
ACGTACGCCT CGAAGTCGGC GTTGCAAACG CTGGACGTTT CTAGTGAGCT CAAGTCTTTG 
GAAGCTGCGA TCGCGGGCTT ATCCAAGGAT CGTGACTCAT ACGCGACGGC GACTCAACTC 
AAGTTGCTTG ACGCCCAAGT CAAGGCTCTT GGAACGCAAA AGGGCAGCAA GGTTGCCGGT 
TTGTTCAAGC GCGACGCGGA TACGGCTACG TTGAAGAAGG ACTTGAAGGC CATTGAGACG 
ACGCTCGTGT CCTTGACGAA GAGTCAAGGT GCGTTTGCGA CATCCGCGCA GTTGAAGGAA 
CTCGTCGACG CCGTCGACGC CTTGCGAAAG TCGTCGAGTG GGTACGCTAG CTCGGATGTC 
GTTGAGACTT TGAAGGTGAG TGTGACGGAG CTGACGGATA AGACGAAGTC GGGATCCGGT 
TCGGCGTCGA AGAACCTTCG TGATTTGGAA CAGCAAGTGA TGGAGCGCAT AGAAGGCTAC 
ATCAACGCCA TTCCGAAGGA CACGTCGATG AAGCTCAAGA AGCACATTGA AAAAGCCGCG 
ACGTTGTGGT TTGCAGATAG GACCGGTCGA CAAGATTTTG CGCTATCCAC CGGTGGCGGA 
CGCGTCGTCG GACACTCGCA ACTTACGCCG TTCGTTGCTC GAGGCGATGG ACCGCTGATG 
TCGGCTGTTT CGTTCCTTCG ATCTGGAGTA CATCCAAAAA GCGATGAGTG GATGCTCACC 
CCCTCGATGG AACAGCCGGG CGATTGTCTT GCACTGCATG GATCTTACGG ATATGTCGAT 
GTTCGTCTGC GTCAACCCGT CAAGGTGGAT GCGGTTACTC TCGAGCACAC CAATAGCCTA 
AACGCGTACG ACATGCACAG CGCTCCACGA GACATACAAA TATTCGGCTG GTACGCCCAT 
GGCAATAACT GCAAGCATAG CAAACCACCA AAGTCATTAA TTGCGATGGG AAATTACACG 
TATCACACGG CAGGGGACAG TGTGCAGTCG TTTGAGGTCA GCGCGCCTCA TACGGTCGAT 
CACGTGCGTC TTATAGTGAA AAACAACCAC GGGCACGCGC GATGGACGTG CATATATCGC 
TTCCGCGTGC ATGGCATACC GTCGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OT05G03050 ( XM_022984286.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_022839032.1 Ostreococcus tauri Sad1/UNC-like, C-terminal (OT_ostta05g02920), partial mRNA.

Price & Availability↑

CloneID	OOd75287
Clone ID Related Accession (Same CDS sequence)	XM_022984286.1
Accession Version	XM_022984286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509984000000
Organism	Ostreococcus tauri
Product	Sad1/UNC-like, C-terminal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014430). COMPLETENESS: incomplete on both ends.

ATGTCCGCCG CGCGCAGGCG CTCGTCCTCG CGCGCGTCGA CGCGGCGTCA GTCCCCAGCG 
AGCGCGGAGG AAAACGAATA TGAAGAATTT GAAGAAATCG TCGACGTGGA GACGCGCCGC 
GAGGTCGTCG CGATGAAGAC CACGGCGGTG CTCAACGCGA AGGTTCCTTT CGCCGTCGCC 
GCGTCGCTGG TGATCGCGAC CGCGGGATGC GCGACGGCGG CGCTGGGATG GCACGCGCGA 
GAGACGGCGT TGGCGCTCGC GAGCGCGCGG GTGAGCGCGA CGAGCGTCGA TTTAGGGCCG 
GCGACGCGAA GGATACGGGT GCTTGAGGAG CAAGTGGAAG CCCTTCAAAG GAATCGCGAG 
GATGGTCGCA AATCGGCGGA GCGAGTGGTG CGCGAGGCCG TGCGTACGTT CGAGCTCGAG 
CGGGTGGAGT GGACGACCAA GGCAGAGGCG GAGGCGAGAA AGGGCGAGAC GCGCGTCACG 
CAGGAGATCA AGGCGGTCGC GGAGTCCGTG TCGGCGCTCA CGGAGAAGGT GCGAAAAACC 
GCTAAGCTCA AGAGCGAGAT TACGAGACTA GAGGCGAGCG TGGCAAAGTT GAAGAGCGCC 
CAAGCGTCGC TCGACAAATC GAGCAAGTCA TCCTTTGCGA GCTCAAAAGA TCTCGAAAAG 
ATTGCGAGCT CGGTAGCAGT CTTAGGTGAA CGACAGGTGG AGTTCGTCAA AACAACGGAA 
ATCGCCGCGC TCCGGAAGGC GCTCGAGGAG TTGGCGTCAA CACAAAAGTC GGTTGACGCG 
CGAGCCACGT CTGAAGTGAA GAGCTTGCGG GATGATATCA CCTCTCTGAA GACGAATATT 
CAGGAGACGT ACGCCTCGAA GTCGGCGTTG CAAACGCTGG ACGTTTCTAG TGAGCTCAAG 
TCTTTGGAAG CTGCGATCGC GGGCTTATCC AAGGATCGTG ACTCATACGC GACGGCGACT 
CAACTCAAGT TGCTTGACGC CCAAGTCAAG GCTCTTGGAA CGCAAAAGGG CAGCAAGGTT 
GCCGGTTTGT TCAATGAGCT CAAGTCTTTG GAAGCTGCGA TCGCGGGCTT ATCCAAGGAT 
CGTGACTCAT ACGCGACGGC GACTCAACTC AAGTTGCTTG ACGCCCAAGT CAAGGCTCTT 
GGAACGCAAA AGGGCAGCAA GGTTGCCGGT TTGTTCAAGC GCGACGCGGA TACGGCTACG 
TTGAAGAAGG ACTTGAAGGC CATCGAGACG ACGCTCGCGT CCTTGACGAA GAGTCAAGGT 
GCGTTTGCGA CATCCGCGCA GTTGAAGGAA CTCGTCGACG CCGTCGACGC CTTGCGAAAG 
TCGTCGAGTG GGTACGCTAG CTCGGATGTC GTTGAGACTT TGAAGGTGAG TGTGACGGAG 
CTGACGAATA AGACGAAGTC GGGATCGATG AAGGAAGTCG CCGCGCTCCG GAAGGCGCTC 
GAGGAGTTGG CGTCAACACA AAAGTCGGTT GACGCGCGAG CCACGTCTGA AGTGAAGAGC 
TTGCGGGATG ACATCACCTC TCTGAAGACG AATATTCAGG AGACGTACGC CTCGAAGTCG 
GCGTTGCAAA CGCTGGACGT TTCTAGTGAG CTCAAGTCTT TGGAAGCTGC GATCGCGGGC 
TTATCCAAGG ATCGTGACTC ATACGCGACG GCGACTCAAC TCAAGTTGCT TGACGCCCAA 
GTCAAGGCTC TTGGAACGCA AAAGGGCAGC AAGGTTGCCG GTTTGTTCAA GCGCGACGCG 
GATACGGCTA CGTTGAAGAA GGACTTGAAG GCCATCGAGA CGACGCTCGT GTCCTTGACG 
AAGAGTCAAG GTGCGTTTGC GACATCCGCG CAGTTGAAGG AACTCGTCGA CGCCGTCGAC 
GCCTTGCGGA AGTCGTCGAG TGGGTACGCT AGCTCGGATG TCGTTGAGAC TTTGAAGGTG 
AGTGTGACGG AGCTGACGAA TAAGACGAAG TCGGGATCGA TGAAGGAAGT CGCCGCGCTC 
CGGAAGGCGC TCGAGGAGTT GGCGTCAACA CAAAAGTCGG TTGACGCGCG AGCCACGTCT 
GAAGTGAAGA GCTTGCGGGA TGATATCACC TCTCTGAAGA CGAATATTCA GGAGACGTAC 
GCCTCGAAGT CGGCGTTGCA AACGCTGGAC GTTTCTAGTG AGCTCAAGTC TTTGGAAGCT 
GCGATCGCGG GCTTATCCAA GGATCGTGAC TCATACGCGA CGGCGACTCA ACTCAAGTTG 
CTTGACGCCC AAGTCAAGGC TCTTGGAACG CAAAAGGGCA GCAAGGTTGC CGGTTTGTTC 
AAGCGCGACG CGGATACGGC TACGTTGAAG AAGGACTTGA AGGCCATCGA GACGACGCTC 
GTGTCCTTGA CGAAGAGTCA AGGTGCGTTT GCGACATCCG CGCAGTTGAA GGAACTCGTC 
GACGCCGTCG ACGCCTTGCG AAAGTCGTCG AGTGGGTACG CTAGCTCGGA TGTCGTTGAG 
ACTTTGAAGG TGAGTGTGAC GGAGCTGACG GATAAGACGA AGTCGGGATC CGGTTCGGCG 
TCGAAGAACC TTCGTGATTT GGAACAGCAA GTGATGGAGC GCATAGAAGG CTACATCAAC 
GCCATTCCGA AGGACACGTC GATGAAGCTC AAGAAGCACA TTGAAAAAGC CGCGACGTTG 
TGGTTTGCAG ATAGGACCGG TCGACAAGAT TTTGCGCTAT CCACCGGTGG CGGACGCGTC 
GTCGGACACT CGCAACTTAC GCCGTTCGTT GCTCGAGGCG ATGGACCGCT GATGTCGGCT 
GTTTCGTTCC TTCGATCTGG AGTACATCCA AAAAGCGATG AGTGGATGCT CACCCCCTCG 
ATGGAACAGC CGGGCGATTG TCTTGCACTG CATGGATCTT ACGGATATGT CGATGTTCGT 
CTGCGTCAAC CCGTCAAGGT GGATGCGGTT ACTCTCGAGC ACACCAATAG CCTAAACGCG 
TACGACATGC ACAGCGCTCC ACGAGACATA CAAATATTCG GCTGGTACGC CCATGGCAAT 
AACTGCAAGC ATAGCAAACC ACCAAAGTCA TTAATTGCGA TGGGAAATTA CACGTATCAC 
ACGGCAGGGG ACAGTGTGCA GTCGTTTGAG GTCAGCGCGC CTCATACGGT CGATCACGTG 
CGTCTTATAG TGAAAAACAA CCACGGGCAC GCGCGATGGA CGTGCATATA TCGCTTCCGC 
GTGCATGGCA TACCGTCGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022839032.1
CDS	1..3204
Translation	MSAARRRSSSRASTRRQSPASAEENEYEEFEEIVDVETRREVVAMKTTAVLNAKVPFAVAASLVIATAGCATAALGWHARETALALASARVSATSVDLGPATRRIRVLEEQVEALQRNREDGRKSAERVVREAVRTFELERVEWTTKAEAEARKGETRVTQEIKAVAESVSALTEKVRKTAKLKSEITRLEASVAKLKSAQASLDKSSKSSFASSKDLEKIASSVAVLGERQVEFVKTTEIAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFNELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLASLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTNKTKSGSMKEVAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLVSLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTNKTKSGSMKEVAALRKALEELASTQKSVDARATSEVKSLRDDITSLKTNIQETYASKSALQTLDVSSELKSLEAAIAGLSKDRDSYATATQLKLLDAQVKALGTQKGSKVAGLFKRDADTATLKKDLKAIETTLVSLTKSQGAFATSAQLKELVDAVDALRKSSSGYASSDVVETLKVSVTELTDKTKSGSGSASKNLRDLEQQVMERIEGYINAIPKDTSMKLKKHIEKAATLWFADRTGRQDFALSTGGGRVVGHSQLTPFVARGDGPLMSAVSFLRSGVHPKSDEWMLTPSMEQPGDCLALHGSYGYVDVRLRQPVKVDAVTLEHTNSLNAYDMHSAPRDIQIFGWYAHGNNCKHSKPPKSLIAMGNYTYHTAGDSVQSFEVSAPHTVDHVRLIVKNNHGHARWTCIYRFRVHGIPSS

Target ORF information:

RefSeq Version	XM_022984286.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri Sad1/UNC-like, C-terminal (OT_ostta05g02920), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022984286.1

ORF Insert Sequence:

ATGTCCGCCG CGCGCAGGCG CTCGTCCTCG CGCGCGTCGA CGCGGCGTCA GTCCCCAGCG 
AGCGCGGAGG AAAACGAATA TGAAGAATTT GAAGAAATCG TCGACGTGGA GACGCGCCGC 
GAGGTCGTCG CGATGAAGAC CACGGCGGTG CTCAACGCGA AGGTTCCTTT CGCCGTCGCC 
GCGTCGCTGG TGATCGCGAC CGCGGGATGC GCGACGGCGG CGCTGGGATG GCACGCGCGA 
GAGACGGCGT TGGCGCTCGC GAGCGCGCGG GTGAGCGCGA CGAGCGTCGA TTTAGGGCCG 
GCGACGCGAA GGATACGGGT GCTTGAGGAG CAAGTGGAAG CCCTTCAAAG GAATCGCGAG 
GATGGTCGCA AATCGGCGGA GCGAGTGGTG CGCGAGGCCG TGCGTACGTT CGAGCTCGAG 
CGGGTGGAGT GGACGACCAA GGCAGAGGCG GAGGCGAGAA AGGGCGAGAC GCGCGTCACG 
CAGGAGATCA AGGCGGTCGC GGAGTCCGTG TCGGCGCTCA CGGAGAAGGT GCGAAAAACC 
GCTAAGCTCA AGAGCGAGAT TACGAGACTA GAGGCGAGCG TGGCAAAGTT GAAGAGCGCC 
CAAGCGTCGC TCGACAAATC GAGCAAGTCA TCCTTTGCGA GCTCAAAAGA TCTCGAAAAG 
ATTGCGAGCT CGGTAGCAGT CTTAGGTGAA CGACAGGTGG AGTTCGTCAA AACAACGGAA 
ATCGCCGCGC TCCGGAAGGC GCTCGAGGAG TTGGCGTCAA CACAAAAGTC GGTTGACGCG 
CGAGCCACGT CTGAAGTGAA GAGCTTGCGG GATGATATCA CCTCTCTGAA GACGAATATT 
CAGGAGACGT ACGCCTCGAA GTCGGCGTTG CAAACGCTGG ACGTTTCTAG TGAGCTCAAG 
TCTTTGGAAG CTGCGATCGC GGGCTTATCC AAGGATCGTG ACTCATACGC GACGGCGACT 
CAACTCAAGT TGCTTGACGC CCAAGTCAAG GCTCTTGGAA CGCAAAAGGG CAGCAAGGTT 
GCCGGTTTGT TCAATGAGCT CAAGTCTTTG GAAGCTGCGA TCGCGGGCTT ATCCAAGGAT 
CGTGACTCAT ACGCGACGGC GACTCAACTC AAGTTGCTTG ACGCCCAAGT CAAGGCTCTT 
GGAACGCAAA AGGGCAGCAA GGTTGCCGGT TTGTTCAAGC GCGACGCGGA TACGGCTACG 
TTGAAGAAGG ACTTGAAGGC CATCGAGACG ACGCTCGCGT CCTTGACGAA GAGTCAAGGT 
GCGTTTGCGA CATCCGCGCA GTTGAAGGAA CTCGTCGACG CCGTCGACGC CTTGCGAAAG 
TCGTCGAGTG GGTACGCTAG CTCGGATGTC GTTGAGACTT TGAAGGTGAG TGTGACGGAG 
CTGACGAATA AGACGAAGTC GGGATCGATG AAGGAAGTCG CCGCGCTCCG GAAGGCGCTC 
GAGGAGTTGG CGTCAACACA AAAGTCGGTT GACGCGCGAG CCACGTCTGA AGTGAAGAGC 
TTGCGGGATG ACATCACCTC TCTGAAGACG AATATTCAGG AGACGTACGC CTCGAAGTCG 
GCGTTGCAAA CGCTGGACGT TTCTAGTGAG CTCAAGTCTT TGGAAGCTGC GATCGCGGGC 
TTATCCAAGG ATCGTGACTC ATACGCGACG GCGACTCAAC TCAAGTTGCT TGACGCCCAA 
GTCAAGGCTC TTGGAACGCA AAAGGGCAGC AAGGTTGCCG GTTTGTTCAA GCGCGACGCG 
GATACGGCTA CGTTGAAGAA GGACTTGAAG GCCATCGAGA CGACGCTCGT GTCCTTGACG 
AAGAGTCAAG GTGCGTTTGC GACATCCGCG CAGTTGAAGG AACTCGTCGA CGCCGTCGAC 
GCCTTGCGGA AGTCGTCGAG TGGGTACGCT AGCTCGGATG TCGTTGAGAC TTTGAAGGTG 
AGTGTGACGG AGCTGACGAA TAAGACGAAG TCGGGATCGA TGAAGGAAGT CGCCGCGCTC 
CGGAAGGCGC TCGAGGAGTT GGCGTCAACA CAAAAGTCGG TTGACGCGCG AGCCACGTCT 
GAAGTGAAGA GCTTGCGGGA TGATATCACC TCTCTGAAGA CGAATATTCA GGAGACGTAC 
GCCTCGAAGT CGGCGTTGCA AACGCTGGAC GTTTCTAGTG AGCTCAAGTC TTTGGAAGCT 
GCGATCGCGG GCTTATCCAA GGATCGTGAC TCATACGCGA CGGCGACTCA ACTCAAGTTG 
CTTGACGCCC AAGTCAAGGC TCTTGGAACG CAAAAGGGCA GCAAGGTTGC CGGTTTGTTC 
AAGCGCGACG CGGATACGGC TACGTTGAAG AAGGACTTGA AGGCCATCGA GACGACGCTC 
GTGTCCTTGA CGAAGAGTCA AGGTGCGTTT GCGACATCCG CGCAGTTGAA GGAACTCGTC 
GACGCCGTCG ACGCCTTGCG AAAGTCGTCG AGTGGGTACG CTAGCTCGGA TGTCGTTGAG 
ACTTTGAAGG TGAGTGTGAC GGAGCTGACG GATAAGACGA AGTCGGGATC CGGTTCGGCG 
TCGAAGAACC TTCGTGATTT GGAACAGCAA GTGATGGAGC GCATAGAAGG CTACATCAAC 
GCCATTCCGA AGGACACGTC GATGAAGCTC AAGAAGCACA TTGAAAAAGC CGCGACGTTG 
TGGTTTGCAG ATAGGACCGG TCGACAAGAT TTTGCGCTAT CCACCGGTGG CGGACGCGTC 
GTCGGACACT CGCAACTTAC GCCGTTCGTT GCTCGAGGCG ATGGACCGCT GATGTCGGCT 
GTTTCGTTCC TTCGATCTGG AGTACATCCA AAAAGCGATG AGTGGATGCT CACCCCCTCG 
ATGGAACAGC CGGGCGATTG TCTTGCACTG CATGGATCTT ACGGATATGT CGATGTTCGT 
CTGCGTCAAC CCGTCAAGGT GGATGCGGTT ACTCTCGAGC ACACCAATAG CCTAAACGCG 
TACGACATGC ACAGCGCTCC ACGAGACATA CAAATATTCG GCTGGTACGC CCATGGCAAT 
AACTGCAAGC ATAGCAAACC ACCAAAGTCA TTAATTGCGA TGGGAAATTA CACGTATCAC 
ACGGCAGGGG ACAGTGTGCA GTCGTTTGAG GTCAGCGCGC CTCATACGGT CGATCACGTG 
CGTCTTATAG TGAAAAACAA CCACGGGCAC GCGCGATGGA CGTGCATATA TCGCTTCCGC 
GTGCATGGCA TACCGTCGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
BMC genomics151103(2014 Dec)
Blanc-Mathieu R,Verhelst B,Derelle E,Rombauts S,Bouget FY,Carré I,Château A,Eyre-Walker A,Grimsley N,Moreau H,Piégu B,Rivals E,Schackwitz W,Van de Peer Y,Piganeau G

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)
Derelle E,Ferraz C,Rombauts S,Rouzé P,Worden AZ,Robbens S,Partensky F,Degroeve S,Echeynié S,Cooke R,Saeys Y,Wuyts J,Jabbari K,Bowler C,Panaud O,Piégu B,Ball SG,Ral JP,Bouget FY,Piganeau G,De Baets B,Picard A,Delseny M,Demaille J,Van de Peer Y,Moreau H

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OT05G03050 cDNA ORF clone, Ostreococcus tauri

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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