LOC100169177 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100169177
Entrez Gene ID	100169177
Full Name	muscle M-line assembly protein unc-89
Gene Type	protein-coding
Organism	Acyrthosiphon pisum(pea aphid)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008184935.2	XP_008183157.1	muscle M-line assembly protein unc-89
XM_029487060.1	XP_029342920.1	muscle M-line assembly protein unc-89 isoform X2
XM_029487061.1	XP_029342921.1	muscle M-line assembly protein unc-89 isoform X3
XM_029487062.1	XP_029342922.1	titin isoform X4
XM_008184935.3	XP_008183157.1	muscle M-line assembly protein unc-89 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100169177 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100169177 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAd09620	XM_029487062.1 Latest version!	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAd09617	XM_008184935.3 Latest version!	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAd09618	XM_029487060.1 Latest version!	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAd09619	XM_029487061.1 Latest version!	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OAd09617	XM_008184935.2	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100169177 ( XM_029487062.1 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_029342922.1 Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAd09620
Clone ID Related Accession (Same CDS sequence)	XM_029487062.1
Accession Version	XM_029487062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	titin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGC TGAGTTTTTG 
GAAAACGAAC GCCGTCAACC GCACCGGGCA CATCCGACGC AGTGGGCAAA CCAGTACAAT 
CAACACAGTC GGACTCAAGT CCGGAGCCCC GCGAAGTTCG TCAGGGGTGA ATTCCGAGAG 
AGCGATTACG AGAGCGACTA CGAGTGCAGG ATACAACCAA TATGGCGCCC GTACGATTCG 
GAATCGGAGG AACCGGTGTA CAAGCCGGTC AGGCCTGGTT TCAAGACTCC CGTAAAAAAG 
CCCGAGGGGT CGCGACTCTT GCAGCCAGTG CAGCGATCGG CGGTGCCGCC GGTGGTGGCT 
TTCGAACTGA AACCAGGGTC ACCGCCCGAA ATGGGTTTCG CGCCGTCGCC GGACGTGCAT 
AGGTCGGTGA GCTTCGTCGA GTCCGAACGC GACGAAAGCG TGCAGAGGAC CACACACTTC 
TTGTCGTCCG ACGGTCGCAG TCACGGGACG CTGGAGCGAA ATGCCGAGGC GAAGCGGTTA 
CAAAGGGTAG ACGAGATGAG GAAGAGGTTC GAACAAAAGT CACTGTGTCC GGCCGAATCG 
AGTTCGGTGC AACAGACGGT CTCGTCTCAA CTCGGGCAAG GTACTAACCA AATCGATTTG 
TCCCAAACCG GAATTCCGTA TTTCAAACAC GATTTCGGAT ATGAGTTCGG TGTAGTGTAT 
CCCGTGAACA CGAGAAACGG TAAAACGGCT AACCCGACGG ATAGCGTCAG CATACCAGTG 
ATACACGAGT TTTCCAAATC CGTTCGGACA CAGACATCCG AGATGTCTAA TTTTAACACA 
CGCCGAGAAC ATTTTAAGAC AACAACAGAT GGAACCGTTA TTAGCCCCGT TTACCAAAAA 
GAACTGAACG CCGCCGAACA CTCGGATCCA TCGCACAGTT CACCTTTCTT CGTAACCCCG 
TTAAAAGACA TAGCTGTAGT TAGCGGCAAG ACTGCCAGAT TCGAGTGCAT AGTGCAAGGT 
GAACCCGTCA GTGAAATCGT GTGGTCGTGT AACGGCCAAG TGCTGGAGAA CTCGGAGTGC 
TACCAGGCGC AGTACAGGAA CGGCGTGTGC AGACTGACGA TACCCAGGAC GTACCAATTC 
AATGCCGGAT CGTACTCGTG CACCGTATCC AATTACCTGG GGTCCAGCAG CACGTCGGCC 
GACCTCCGAG TGTCAGGTGA GTCTCGCACG TTCATTCAAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029342922.1
CDS	605..8329
Translation	MESDDDYYTVSECLSSKVSSYGSCTPIASDQDEEFDTDTVVREVLQTRKLMLSPVRGCVETSEYIYKDTPILRTEKQPKCRDVLELKVLELCPELTLLGETLEEASSLQAEHERVLEKIQEKQSPVNELLKKADYLIATQNTQPQVYAAMADSLGLAWKDVNDVLQKRSYLLHLNVEYHRQAEVCMERIGTLEMMCHSYLPTDVESIKNHLEHIQEVRKSILEALMVALGNGTQLLDVLKEMQAKGTLDSRPGYNYHSISLAVSQVEHWLEKLHDKRHSLDIDCHERKTMLEKRLTISLLVTDLNLLENILSERRNSLERVKNCLGDSQYECSKFTEVNSNLITEAKDIRDQALRVARATEQLVLTKGYMEEDYKFKAYKLLELATEYLEELNRRETLLDAAMKFFESAEKVLRAWETILQHSSDEQLASKNTYVTETVKRTGASVIQEGNLILKDLPEAEGVKRVVDELERKKQLILSIVMKDRDKYMESSEAVNTFSENYNKIFSWLVSLAESFLHEHNSMGTNLTEAREFLISHKTLQRDLRTRGIDINALVNSTSLPPLTYIDAETRVDLEGKSIVLKDHWTYLDRILEWRIQLSELYVKFYTSCEHLNIEFSNLDSIIGDHVDVSDQRLDSAHKYWTNILQLYIQLKNTGEKFLHQSSKSEDAHLELDVARDRVRSLLDTFVDRKTRTTSVWETWQRASAENKLDKVLWSETMCESSKTVDWVSKLESQLYPVLKSEPSSVDRHISEVKQKLNNVLPEVQRAQDDIESRIKTAEELIGKGNIQGDNISIPPRLKELHSRLVGITTEYQNLLDMFLSFFNSLQEFHDIVKESERGRPTDIFNHQLNEVEELLKKHNQSRQTVLDSFKTVYAENIRLMDKIKQQEPQKSGEQDMYVVRYLMENERNLWDQSCETYCQRIEQQQQLSQFDNDLIQINNNLNDLSQQLSSTRGQYGANLASAKSASLAFHYFEKTILLLEQRIETFINAANSLMNFDHNKSEHIREELNKLKSRWDTFQAQVLESRNHIDLCVQYFTLINEAQDWFHEGSILLMNIARKSTEVKTSEEASALLKEIEIFLKPGDIRQDERLNQIHELSVQLFGEHAIAEVPQVVTENKELLDSFSLIAKELKSFDDKLKSREKYEINNEVNQIITDTYEEIITSTSMTNGVSQNEGNVPLSKKIKLEEIHKPKPTIILPLQDNTVDEGKQFTFECSIEGEPSKVTWFKDNISIDNNPDYQTSKIDNRYILRIEETFVEDSAKFTCRAENDSDFIETSAYLSVKECAPEHQISPSVFTKLLSDLLVKEGDACSLSCKAEGNPLPTVQWFKDDICIDNSPQYQITYNNGEALLKIEHTNSSTHSGKYTCVATNRLGSDSTASKLLIDALEKPEDGPTFVVPLSNVMARAGQKLKLECEVETNKPPILIWFHNGKVMKEIKDFKVSENRGKINLIIPEAFPKDAGTYSLTIKTEQGEATSSCQVSVKGILPNETSDSEIASDLEPIKPSIPLLLKEQTVLEGKSVHLKCIIVGQPEPEVIWYHDGRPVKESKDIQLLFQGDQCSLVIKEAFIEDAGEYKVVAINSAGEASSTCVLSVEPKAVPDIDELVVSPKFTKLLSDVLVREGDSITLECNAEGCPKPEFKWIRNTVEIKPDQRITLSSDEDGTATLHIHNALSVDKGQYTVRAVNKAGEAKCFSHVIVKAISTFDTAVNPPTDKVQFEEKLEKPSFTETFPSVVNAFTGESVKFECIVVGKPAPKVRWLFNEKPVSGKSFLVSTSGDRQVLTIPEADTGHGGIVECVAENEAGKCKCVSSLKIKESEAVPLTMAYNGGGDLKHTVSEVYESSSKFESKQSTFVSSSSSISGDNAPKFKLDSFSLQSEKQSRQFGDNAPIQSESLRTEEYHNTDGKQSQHVSDLSSTNVSEEHQTSFFERGLYNQSGLVKDASQKSLLERNISKQSRKEMAPRFVSAPQGKIAEQGKDVFLDAIIDSYPPSDITWSKNGIDLVPDGVKLKITSEVNKTRLDIKRLTVEDGGQYTCKAMNTAGGTSCTTDVIVKKNVFPPVMCRRLLPNTVAEGERVVLEIEVAGTPEPTVSWFKNNQPLSATSSLYRLRQQGSCSAVVIDKATTEHAGEYKVMAKNEGGEAVSCADLRVVQPLPDIGLNLLPTYAPNDNTDKVNVAEFLENERRQPHRAHPTQWANQYNQHSRTQVRSPAKFVRGEFRESDYESDYECRIQPIWRPYDSESEEPVYKPVRPGFKTPVKKPEGSRLLQPVQRSAVPPVVAFELKPGSPPEMGFAPSPDVHRSVSFVESERDESVQRTTHFLSSDGRSHGTLERNAEAKRLQRVDEMRKRFEQKSLCPAESSSVQQTVSSQLGQGTNQIDLSQTGIPYFKHDFGYEFGVVYPVNTRNGKTANPTDSVSIPVIHEFSKSVRTQTSEMSNFNTRREHFKTTTDGTVISPVYQKELNAAEHSDPSHSSPFFVTPLKDIAVVSGKTARFECIVQGEPVSEIVWSCNGQVLENSECYQAQYRNGVCRLTIPRTYQFNAGSYSCTVSNYLGSSSTSADLRVSGESRTFIQH

Target ORF information:

RefSeq Version	XM_029487062.1
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029487062.1

ORF Insert Sequence:

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGC TGAGTTTTTG 
GAAAACGAAC GCCGTCAACC GCACCGGGCA CATCCGACGC AGTGGGCAAA CCAGTACAAT 
CAACACAGTC GGACTCAAGT CCGGAGCCCC GCGAAGTTCG TCAGGGGTGA ATTCCGAGAG 
AGCGATTACG AGAGCGACTA CGAGTGCAGG ATACAACCAA TATGGCGCCC GTACGATTCG 
GAATCGGAGG AACCGGTGTA CAAGCCGGTC AGGCCTGGTT TCAAGACTCC CGTAAAAAAG 
CCCGAGGGGT CGCGACTCTT GCAGCCAGTG CAGCGATCGG CGGTGCCGCC GGTGGTGGCT 
TTCGAACTGA AACCAGGGTC ACCGCCCGAA ATGGGTTTCG CGCCGTCGCC GGACGTGCAT 
AGGTCGGTGA GCTTCGTCGA GTCCGAACGC GACGAAAGCG TGCAGAGGAC CACACACTTC 
TTGTCGTCCG ACGGTCGCAG TCACGGGACG CTGGAGCGAA ATGCCGAGGC GAAGCGGTTA 
CAAAGGGTAG ACGAGATGAG GAAGAGGTTC GAACAAAAGT CACTGTGTCC GGCCGAATCG 
AGTTCGGTGC AACAGACGGT CTCGTCTCAA CTCGGGCAAG GTACTAACCA AATCGATTTG 
TCCCAAACCG GAATTCCGTA TTTCAAACAC GATTTCGGAT ATGAGTTCGG TGTAGTGTAT 
CCCGTGAACA CGAGAAACGG TAAAACGGCT AACCCGACGG ATAGCGTCAG CATACCAGTG 
ATACACGAGT TTTCCAAATC CGTTCGGACA CAGACATCCG AGATGTCTAA TTTTAACACA 
CGCCGAGAAC ATTTTAAGAC AACAACAGAT GGAACCGTTA TTAGCCCCGT TTACCAAAAA 
GAACTGAACG CCGCCGAACA CTCGGATCCA TCGCACAGTT CACCTTTCTT CGTAACCCCG 
TTAAAAGACA TAGCTGTAGT TAGCGGCAAG ACTGCCAGAT TCGAGTGCAT AGTGCAAGGT 
GAACCCGTCA GTGAAATCGT GTGGTCGTGT AACGGCCAAG TGCTGGAGAA CTCGGAGTGC 
TACCAGGCGC AGTACAGGAA CGGCGTGTGC AGACTGACGA TACCCAGGAC GTACCAATTC 
AATGCCGGAT CGTACTCGTG CACCGTATCC AATTACCTGG GGTCCAGCAG CACGTCGGCC 
GACCTCCGAG TGTCAGGTGA GTCTCGCACG TTCATTCAAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100169177 ( XM_008184935.2 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_008183157.1 Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), mRNA.

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CloneID	OAd09617
Clone ID Related Accession (Same CDS sequence)	XM_008184935.2 , XM_008184935.3
Accession Version	XM_008184935.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463328000000
Organism	Acyrthosiphon pisum(pea aphid)
Product	muscle M-line assembly protein unc-89
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2016 this sequence version replaced XM_008184935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acyrthosiphon pisum Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGGTACTAA CCAAATCGAT 
TTGTCCCAAA CCGGAATTCC GTATTTCAAA CACGATTTCG GATATGAGTT CGGTGTAGTG 
TATCCCGTGA ACACGAGAAA CGGTAAAACG GCTAACCCGA CGGATAGCGT CAGCATACCA 
GTGATACACG AGTTTTCCAA ATCCGTTCGG ACACAGACAT CCGAGATGTC TAATTTTAAC 
ACACGCCGAG AACATTTTAA GACAACAACA GATGGAACCG TTATTAGCCC CGTTTACCAA 
AAAGAACTGA ACGCCGCCGA ACACTCGGAT CCATCGCACA GTTCACCTTT CTTCGTAACC 
CCGTTAAAAG ACATAGCTGT AGTTAGCGGC AAGACTGCCA GATTCGAGTG CATAGTGCAA 
GGTGAACCCG TCAGTGAAAT CGTGTGGTCG TGTAACGGCC AAGTGCTGGA GAACTCGGAG 
TGCTACCAGG CGCAGTACAG GAACGGCGTG TGCAGACTGA CGATACCCAG GACGTACCAA 
TTCAATGCCG GATCGTACTC GTGCACCGTA TCCAATTACC TGGGGTCCAG CAGCACGTCG 
GCCGACCTCC GAGTGTCAGG TGAGTCTCGC ACGTTCATTC AACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008183157.1
CDS	46..9873
Translation	MESDDDYYTVSECLSSKVSSYGSCTPIASDQDEEFDTDTVVREVLQTRKLMLSPVRGCVETSEYIYKDTPILRTEKQPKCRDVLELKVLELCPELTLLGETLEEASSLQAEHERVLEKIQEKQSPVNELLKKADYLIATQNTQPQVYAAMADSLGLAWKDVNDVLQKRSYLLHLNVEYHRQAEVCMERIGTLEMMCHSYLPTDVESIKNHLEHIQEVRKSILEALMVALGNGTQLLDVLKEMQAKGTLDSRPGYNYHSISLAVSQVEHWLEKLHDKRHSLDIDCHERKTMLEKRLTISLLVTDLNLLENILSERRNSLERVKNCLGDSQYECSKFTEVNSNLITEAKDIRDQALRVARATEQLVLTKGYMEEDYKFKAYKLLELATEYLEELNRRETLLDAAMKFFESAEKVLRAWETILQHSSDEQLASKNTYVTETVKRTGASVIQEGNLILKDLPEAEGVKRVVDELERKKQLILSIVMKDRDKYMESSEAVNTFSENYNKIFSWLVSLAESFLHEHNSMGTNLTEAREFLISHKTLQRDLRTRGIDINALVNSTSLPPLTYIDAETRVDLEGKSIVLKDHWTYLDRILEWRIQLSELYVKFYTSCEHLNIEFSNLDSIIGDHVDVSDQRLDSAHKYWTNILQLYIQLKNTGEKFLHQSSKSEDAHLELDVARDRVRSLLDTFVDRKTRTTSVWETWQRASAENKLDKVLWSETMCESSKTVDWVSKLESQLYPVLKSEPSSVDRHISEVKQKLNNVLPEVQRAQDDIESRIKTAEELIGKGNIQGDNISIPPRLKELHSRLVGITTEYQNLLDMFLSFFNSLQEFHDIVKESERGRPTDIFNHQLNEVEELLKKHNQSRQTVLDSFKTVYAENIRLMDKIKQQEPQKSGEQDMYVVRYLMENERNLWDQSCETYCQRIEQQQQLSQFDNDLIQINNNLNDLSQQLSSTRGQYGANLASAKSASLAFHYFEKTILLLEQRIETFINAANSLMNFDHNKSEHIREELNKLKSRWDTFQAQVLESRNHIDLCVQYFTLINEAQDWFHEGSILLMNIARKSTEVKTSEEASALLKEIEIFLKPGDIRQDERLNQIHELSVQLFGEHAIAEVPQVVTENKELLDSFSLIAKELKSFDDKLKSREKYEINNEVNQIITDTYEEIITSTSMTNGVSQNEGNVPLSKKIKLEEIHKPKPTIILPLQDNTVDEGKQFTFECSIEGEPSKVTWFKDNISIDNNPDYQTSKIDNRYILRIEETFVEDSAKFTCRAENDSDFIETSAYLSVKECAPEHQISPSVFTKLLSDLLVKEGDACSLSCKAEGNPLPTVQWFKDDICIDNSPQYQITYNNGEALLKIEHTNSSTHSGKYTCVATNRLGSDSTASKLLIDALEKPEDGPTFVVPLSNVMARAGQKLKLECEVETNKPPILIWFHNGKVMKEIKDFKVSENRGKINLIIPEAFPKDAGTYSLTIKTEQGEATSSCQVSVKGILPNETSDSEIASDLEPIKPSIPLLLKEQTVLEGKSVHLKCIIVGQPEPEVIWYHDGRPVKESKDIQLLFQGDQCSLVIKEAFIEDAGEYKVVAINSAGEASSTCVLSVEPKAVPDIDELVVSPKFTKLLSDVLVREGDSITLECNAEGCPKPEFKWIRNTVEIKPDQRITLSSDEDGTATLHIHNALSVDKGQYTVRAVNKAGEAKCFSHVIVKAISTFDTAVNPPTDKVQFEEKLEKPSFTETFPSVVNAFTGESVKFECIVVGKPAPKVRWLFNEKPVSGKSFLVSTSGDRQVLTIPEADTGHGGIVECVAENEAGKCKCVSSLKIKESEAVPLTMAYNGGGDLKHTVSEVYESSSKFESKQSTFVSSSSSISGDNAPKFKLDSFSLQSEKQSRQFGDNAPIQSESLRTEEYHNTDGKQSQHVSDLSSTNVSEEHQTSFFERGLYNQSGLVKDASQKSLLERNISKQSRKEMAPRFVSAPQGKIAEQGKDVFLDAIIDSYPPSDITWSKNGIDLVPDGVKLKITSEVNKTRLDIKRLTVEDGGQYTCKAMNTAGGTSCTTDVIVKKNVFPPVMCRRLLPNTVAEGERVVLEIEVAGTPEPTVSWFKNNQPLSATSSLYRLRQQGSCSAVVIDKATTEHAGEYKVMAKNEGGEAVSCADLRVVQPLPDIGLNLLPTYAPNDNTDKVNVDYSKSIVPTNQSKQFNITSKTEEKSHFSKSVFVQESINSSKDVIMTVNRSEANDFDLNSNNANANYENTEKQISVQNENIENASITKKSALQFFKNVIEDNKVEKSSNSTIPPSAGIYKRESPIDNSSNFASDVHNKTYNIEENVSSSSFKQSNDTEVILEPGPPPTFDYMPRAQSVKKDQMTERLRRLSTNQKLLSPEQIPSGAVRIFPDVTSEIKQEVVEESCIKKEVIKSGLSEDTIAHTHLPTSYTPLPHSEPHVPLLRPQADIEVRPGSPRPSAEAISMEKLWTKGHTPHVQKTPVSYSPIHMKTYSSTESQSFVSETIEKNGELVKNESREEKQGSLKIDDGVKKVSESFSEISIRPTKHVEPPRNEIKRPASAHLFNEVKSKDAFFGNKVQTQHFSEESSQIKKSSMFDVKSNISEKVERNSSNVKSEHQLQNNQSFDRNMQNTYQFLPERSIGPIKHVEPPNNSKIISNFTRSHSVETAKEKQWLSQSPVAFRGPPQNKTYSSFEAHSSFSKTLEKDGVLVASEHNEEGGRVVDDGVQKAYDSFSERSKLPTKFVDLSKNTNKSNSIKTMQKMFEQTGSSSTVSNVGSNVRPYSRIKSRASDSDFESEAELSKYNVEQKVSESFNSFETKMYSSKTQLQEIKNTSIIMQSPIKESGYAADTDEPRGFQSESVVNNQTFNSKRNSGFQSTSIKKAEFLENERRQPHRAHPTQWANQYNQHSRTQVRSPAKFVRGEFRESDYESDYECRIQPIWRPYDSESEEPVYKPVRPGFKTPVKKPEGSRLLQPVQRSAVPPVVAFELKPGSPPEMGFAPSPDVHRSVSFVESERDESVQRTTHFLSSDGRSHGTLERNAEAKRLQRVDEMRKRFEQKSLCPAESSSVQQTVSSQLGQGTNQIDLSQTGIPYFKHDFGYEFGVVYPVNTRNGKTANPTDSVSIPVIHEFSKSVRTQTSEMSNFNTRREHFKTTTDGTVISPVYQKELNAAEHSDPSHSSPFFVTPLKDIAVVSGKTARFECIVQGEPVSEIVWSCNGQVLENSECYQAQYRNGVCRLTIPRTYQFNAGSYSCTVSNYLGSSSTSADLRVSGESRTFIQH

Target ORF information:

RefSeq Version	XM_008184935.2
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008184935.2

ORF Insert Sequence:

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGGTACTAA CCAAATCGAT 
TTGTCCCAAA CCGGAATTCC GTATTTCAAA CACGATTTCG GATATGAGTT CGGTGTAGTG 
TATCCCGTGA ACACGAGAAA CGGTAAAACG GCTAACCCGA CGGATAGCGT CAGCATACCA 
GTGATACACG AGTTTTCCAA ATCCGTTCGG ACACAGACAT CCGAGATGTC TAATTTTAAC 
ACACGCCGAG AACATTTTAA GACAACAACA GATGGAACCG TTATTAGCCC CGTTTACCAA 
AAAGAACTGA ACGCCGCCGA ACACTCGGAT CCATCGCACA GTTCACCTTT CTTCGTAACC 
CCGTTAAAAG ACATAGCTGT AGTTAGCGGC AAGACTGCCA GATTCGAGTG CATAGTGCAA 
GGTGAACCCG TCAGTGAAAT CGTGTGGTCG TGTAACGGCC AAGTGCTGGA GAACTCGGAG 
TGCTACCAGG CGCAGTACAG GAACGGCGTG TGCAGACTGA CGATACCCAG GACGTACCAA 
TTCAATGCCG GATCGTACTC GTGCACCGTA TCCAATTACC TGGGGTCCAG CAGCACGTCG 
GCCGACCTCC GAGTGTCAGG TGAGTCTCGC ACGTTCATTC AACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100169177 ( XM_008184935.3 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_008183157.1 Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X1, mRNA.

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CloneID	OAd09617
Clone ID Related Accession (Same CDS sequence)	XM_008184935.2 , XM_008184935.3
Accession Version	XM_008184935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	muscle M-line assembly protein unc-89 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2019 this sequence version replaced XM_008184935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGGTACTAA CCAAATCGAT 
TTGTCCCAAA CCGGAATTCC GTATTTCAAA CACGATTTCG GATATGAGTT CGGTGTAGTG 
TATCCCGTGA ACACGAGAAA CGGTAAAACG GCTAACCCGA CGGATAGCGT CAGCATACCA 
GTGATACACG AGTTTTCCAA ATCCGTTCGG ACACAGACAT CCGAGATGTC TAATTTTAAC 
ACACGCCGAG AACATTTTAA GACAACAACA GATGGAACCG TTATTAGCCC CGTTTACCAA 
AAAGAACTGA ACGCCGCCGA ACACTCGGAT CCATCGCACA GTTCACCTTT CTTCGTAACC 
CCGTTAAAAG ACATAGCTGT AGTTAGCGGC AAGACTGCCA GATTCGAGTG CATAGTGCAA 
GGTGAACCCG TCAGTGAAAT CGTGTGGTCG TGTAACGGCC AAGTGCTGGA GAACTCGGAG 
TGCTACCAGG CGCAGTACAG GAACGGCGTG TGCAGACTGA CGATACCCAG GACGTACCAA 
TTCAATGCCG GATCGTACTC GTGCACCGTA TCCAATTACC TGGGGTCCAG CAGCACGTCG 
GCCGACCTCC GAGTGTCAGG TGAGTCTCGC ACGTTCATTC AACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008183157.1
CDS	605..10432
Translation	MESDDDYYTVSECLSSKVSSYGSCTPIASDQDEEFDTDTVVREVLQTRKLMLSPVRGCVETSEYIYKDTPILRTEKQPKCRDVLELKVLELCPELTLLGETLEEASSLQAEHERVLEKIQEKQSPVNELLKKADYLIATQNTQPQVYAAMADSLGLAWKDVNDVLQKRSYLLHLNVEYHRQAEVCMERIGTLEMMCHSYLPTDVESIKNHLEHIQEVRKSILEALMVALGNGTQLLDVLKEMQAKGTLDSRPGYNYHSISLAVSQVEHWLEKLHDKRHSLDIDCHERKTMLEKRLTISLLVTDLNLLENILSERRNSLERVKNCLGDSQYECSKFTEVNSNLITEAKDIRDQALRVARATEQLVLTKGYMEEDYKFKAYKLLELATEYLEELNRRETLLDAAMKFFESAEKVLRAWETILQHSSDEQLASKNTYVTETVKRTGASVIQEGNLILKDLPEAEGVKRVVDELERKKQLILSIVMKDRDKYMESSEAVNTFSENYNKIFSWLVSLAESFLHEHNSMGTNLTEAREFLISHKTLQRDLRTRGIDINALVNSTSLPPLTYIDAETRVDLEGKSIVLKDHWTYLDRILEWRIQLSELYVKFYTSCEHLNIEFSNLDSIIGDHVDVSDQRLDSAHKYWTNILQLYIQLKNTGEKFLHQSSKSEDAHLELDVARDRVRSLLDTFVDRKTRTTSVWETWQRASAENKLDKVLWSETMCESSKTVDWVSKLESQLYPVLKSEPSSVDRHISEVKQKLNNVLPEVQRAQDDIESRIKTAEELIGKGNIQGDNISIPPRLKELHSRLVGITTEYQNLLDMFLSFFNSLQEFHDIVKESERGRPTDIFNHQLNEVEELLKKHNQSRQTVLDSFKTVYAENIRLMDKIKQQEPQKSGEQDMYVVRYLMENERNLWDQSCETYCQRIEQQQQLSQFDNDLIQINNNLNDLSQQLSSTRGQYGANLASAKSASLAFHYFEKTILLLEQRIETFINAANSLMNFDHNKSEHIREELNKLKSRWDTFQAQVLESRNHIDLCVQYFTLINEAQDWFHEGSILLMNIARKSTEVKTSEEASALLKEIEIFLKPGDIRQDERLNQIHELSVQLFGEHAIAEVPQVVTENKELLDSFSLIAKELKSFDDKLKSREKYEINNEVNQIITDTYEEIITSTSMTNGVSQNEGNVPLSKKIKLEEIHKPKPTIILPLQDNTVDEGKQFTFECSIEGEPSKVTWFKDNISIDNNPDYQTSKIDNRYILRIEETFVEDSAKFTCRAENDSDFIETSAYLSVKECAPEHQISPSVFTKLLSDLLVKEGDACSLSCKAEGNPLPTVQWFKDDICIDNSPQYQITYNNGEALLKIEHTNSSTHSGKYTCVATNRLGSDSTASKLLIDALEKPEDGPTFVVPLSNVMARAGQKLKLECEVETNKPPILIWFHNGKVMKEIKDFKVSENRGKINLIIPEAFPKDAGTYSLTIKTEQGEATSSCQVSVKGILPNETSDSEIASDLEPIKPSIPLLLKEQTVLEGKSVHLKCIIVGQPEPEVIWYHDGRPVKESKDIQLLFQGDQCSLVIKEAFIEDAGEYKVVAINSAGEASSTCVLSVEPKAVPDIDELVVSPKFTKLLSDVLVREGDSITLECNAEGCPKPEFKWIRNTVEIKPDQRITLSSDEDGTATLHIHNALSVDKGQYTVRAVNKAGEAKCFSHVIVKAISTFDTAVNPPTDKVQFEEKLEKPSFTETFPSVVNAFTGESVKFECIVVGKPAPKVRWLFNEKPVSGKSFLVSTSGDRQVLTIPEADTGHGGIVECVAENEAGKCKCVSSLKIKESEAVPLTMAYNGGGDLKHTVSEVYESSSKFESKQSTFVSSSSSISGDNAPKFKLDSFSLQSEKQSRQFGDNAPIQSESLRTEEYHNTDGKQSQHVSDLSSTNVSEEHQTSFFERGLYNQSGLVKDASQKSLLERNISKQSRKEMAPRFVSAPQGKIAEQGKDVFLDAIIDSYPPSDITWSKNGIDLVPDGVKLKITSEVNKTRLDIKRLTVEDGGQYTCKAMNTAGGTSCTTDVIVKKNVFPPVMCRRLLPNTVAEGERVVLEIEVAGTPEPTVSWFKNNQPLSATSSLYRLRQQGSCSAVVIDKATTEHAGEYKVMAKNEGGEAVSCADLRVVQPLPDIGLNLLPTYAPNDNTDKVNVDYSKSIVPTNQSKQFNITSKTEEKSHFSKSVFVQESINSSKDVIMTVNRSEANDFDLNSNNANANYENTEKQISVQNENIENASITKKSALQFFKNVIEDNKVEKSSNSTIPPSAGIYKRESPIDNSSNFASDVHNKTYNIEENVSSSSFKQSNDTEVILEPGPPPTFDYMPRAQSVKKDQMTERLRRLSTNQKLLSPEQIPSGAVRIFPDVTSEIKQEVVEESCIKKEVIKSGLSEDTIAHTHLPTSYTPLPHSEPHVPLLRPQADIEVRPGSPRPSAEAISMEKLWTKGHTPHVQKTPVSYSPIHMKTYSSTESQSFVSETIEKNGELVKNESREEKQGSLKIDDGVKKVSESFSEISIRPTKHVEPPRNEIKRPASAHLFNEVKSKDAFFGNKVQTQHFSEESSQIKKSSMFDVKSNISEKVERNSSNVKSEHQLQNNQSFDRNMQNTYQFLPERSIGPIKHVEPPNNSKIISNFTRSHSVETAKEKQWLSQSPVAFRGPPQNKTYSSFEAHSSFSKTLEKDGVLVASEHNEEGGRVVDDGVQKAYDSFSERSKLPTKFVDLSKNTNKSNSIKTMQKMFEQTGSSSTVSNVGSNVRPYSRIKSRASDSDFESEAELSKYNVEQKVSESFNSFETKMYSSKTQLQEIKNTSIIMQSPIKESGYAADTDEPRGFQSESVVNNQTFNSKRNSGFQSTSIKKAEFLENERRQPHRAHPTQWANQYNQHSRTQVRSPAKFVRGEFRESDYESDYECRIQPIWRPYDSESEEPVYKPVRPGFKTPVKKPEGSRLLQPVQRSAVPPVVAFELKPGSPPEMGFAPSPDVHRSVSFVESERDESVQRTTHFLSSDGRSHGTLERNAEAKRLQRVDEMRKRFEQKSLCPAESSSVQQTVSSQLGQGTNQIDLSQTGIPYFKHDFGYEFGVVYPVNTRNGKTANPTDSVSIPVIHEFSKSVRTQTSEMSNFNTRREHFKTTTDGTVISPVYQKELNAAEHSDPSHSSPFFVTPLKDIAVVSGKTARFECIVQGEPVSEIVWSCNGQVLENSECYQAQYRNGVCRLTIPRTYQFNAGSYSCTVSNYLGSSSTSADLRVSGESRTFIQH

Target ORF information:

RefSeq Version	XM_008184935.3
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008184935.3

ORF Insert Sequence:

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGGTACTAA CCAAATCGAT 
TTGTCCCAAA CCGGAATTCC GTATTTCAAA CACGATTTCG GATATGAGTT CGGTGTAGTG 
TATCCCGTGA ACACGAGAAA CGGTAAAACG GCTAACCCGA CGGATAGCGT CAGCATACCA 
GTGATACACG AGTTTTCCAA ATCCGTTCGG ACACAGACAT CCGAGATGTC TAATTTTAAC 
ACACGCCGAG AACATTTTAA GACAACAACA GATGGAACCG TTATTAGCCC CGTTTACCAA 
AAAGAACTGA ACGCCGCCGA ACACTCGGAT CCATCGCACA GTTCACCTTT CTTCGTAACC 
CCGTTAAAAG ACATAGCTGT AGTTAGCGGC AAGACTGCCA GATTCGAGTG CATAGTGCAA 
GGTGAACCCG TCAGTGAAAT CGTGTGGTCG TGTAACGGCC AAGTGCTGGA GAACTCGGAG 
TGCTACCAGG CGCAGTACAG GAACGGCGTG TGCAGACTGA CGATACCCAG GACGTACCAA 
TTCAATGCCG GATCGTACTC GTGCACCGTA TCCAATTACC TGGGGTCCAG CAGCACGTCG 
GCCGACCTCC GAGTGTCAGG TGAGTCTCGC ACGTTCATTC AACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100169177 ( XM_029487060.1 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_029342920.1 Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X2, mRNA.

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CloneID	OAd09618
Clone ID Related Accession (Same CDS sequence)	XM_029487060.1
Accession Version	XM_029487060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	muscle M-line assembly protein unc-89 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG ACTCCCGGCC GCCGGCCACC ACCGTCAGCA CAATAGCCGT CCGAAGTGGC 
GCCACCAGGA TCGTCATAGC CCTATTACAG TGTCGTGACG TTTTAGAATT AAAAGTTCTC 
GAACTATGCC CGGAACTTAC GTTGCTCGGG GAGACGTTGG AAGAGGCCTC GAGCTTACAG 
GCCGAACACG AGAGAGTGTT GGAAAAAATA CAGGAAAAGC AGAGTCCCGT CAACGAGTTG 
TTGAAGAAAG CCGATTATCT AATAGCTACA CAGAACACGC AGCCCCAGGT GTATGCCGCT 
ATGGCCGATT CGCTGGGATT AGCCTGGAAA GACGTAAACG ATGTACTTCA AAAGAGAAGT 
TACTTGTTAC ACCTGAACGT TGAATATCAC CGACAAGCTG AAGTATGCAT GGAACGAATC 
GGAACTTTGG AAATGATGTG CCACAGTTAC CTGCCGACCG ACGTCGAATC GATCAAAAAC 
CATCTGGAGC ATATCCAGGA AGTCCGCAAG TCTATACTGG AAGCGCTAAT GGTCGCTTTG 
GGCAATGGCA CGCAGCTACT GGACGTTTTG AAAGAGATGC AAGCCAAAGG AACCCTGGAT 
TCGAGGCCGG GATATAATTA TCATTCTATT TCATTAGCCG TGTCTCAAGT AGAACATTGG 
TTGGAGAAAC TTCACGATAA ACGTCATTCG TTGGACATTG ACTGTCACGA ACGTAAGACA 
ATGTTGGAAA AACGACTGAC AATATCTTTA CTAGTCACCG ATCTAAATTT ACTTGAAAAC 
ATACTATCTG AAAGAAGAAA TTCTCTGGAA AGAGTTAAAA ATTGTCTAGG CGACTCCCAG 
TATGAATGTT CTAAATTTAC GGAAGTCAAT TCGAACTTGA TAACCGAAGC AAAGGATATA 
AGAGACCAAG CGCTGAGGGT TGCAAGAGCC ACCGAACAAC TGGTATTAAC TAAAGGCTAC 
ATGGAAGAGG ATTATAAATT TAAAGCGTAT AAACTATTAG AATTAGCTAC CGAATACTTG 
GAAGAACTAA ATAGAAGAGA AACTCTTTTA GATGCCGCTA TGAAATTTTT CGAGTCGGCT 
GAAAAAGTTC TCCGAGCATG GGAAACCATA CTTCAGCATT CATCCGATGA GCAATTGGCA 
TCAAAGAACA CGTACGTTAC AGAGACGGTA AAGCGAACCG GCGCCTCAGT AATTCAAGAA 
GGAAATTTAA TACTGAAAGA CTTACCAGAG GCAGAGGGCG TTAAACGGGT TGTAGACGAG 
TTGGAAAGGA AAAAACAGCT AATACTGTCA ATTGTTATGA AAGACCGCGA CAAATATATG 
GAATCTTCAG AAGCAGTCAA CACGTTTTCA GAGAATTATA ATAAAATATT TTCCTGGCTT 
GTAAGTTTAG CTGAATCGTT TTTACACGAA CATAATTCTA TGGGTACAAA TCTCACGGAA 
GCGCGAGAGT TTTTAATATC CCATAAAACT TTACAAAGAG ATCTAAGGAC TCGAGGGATC 
GACATTAACG CATTGGTAAA CTCGACTAGT CTGCCACCGT TAACGTACAT CGATGCTGAA 
ACAAGAGTCG ATCTGGAAGG CAAATCAATC GTGTTAAAAG ACCATTGGAC TTATTTGGAT 
AGAATATTGG AATGGAGGAT ACAATTGTCC GAGTTGTATG TCAAATTTTA CACGTCGTGT 
GAACATTTGA ACATCGAATT TTCAAACTTG GACTCGATCA TTGGAGATCA CGTCGACGTG 
TCTGATCAAA GACTAGATTC GGCGCACAAG TATTGGACAA ACATTTTACA ATTGTATATC 
CAACTTAAGA ACACAGGAGA AAAGTTTTTG CATCAAAGTT CAAAATCGGA GGACGCTCAT 
CTGGAACTAG ACGTAGCACG GGACCGCGTG CGATCGCTAC TGGACACGTT CGTGGACAGG 
AAAACGAGAA CTACTTCCGT CTGGGAGACT TGGCAGCGGG CATCGGCTGA AAACAAACTG 
GATAAAGTGC TCTGGTCGGA GACGATGTGC GAGAGCAGCA AGACAGTAGA TTGGGTTTCC 
AAATTGGAAT CTCAATTATA TCCAGTTTTG AAATCTGAGC CGTCATCAGT TGATCGACAT 
ATTTCAGAGG TTAAACAGAA GTTAAATAAT GTTCTACCGG AAGTTCAAAG GGCTCAAGAC 
GATATCGAGT CCAGAATAAA AACAGCCGAA GAACTTATTG GAAAAGGAAA TATTCAAGGT 
GATAATATTT CTATACCTCC AAGGTTAAAA GAACTTCATA GCCGTCTTGT TGGAATCACT 
ACAGAGTATC AAAATCTTTT AGATATGTTC TTATCATTTT TTAATTCTCT ACAAGAATTT 
CACGATATTG TGAAAGAATC TGAAAGAGGT AGGCCCACCG ACATTTTCAA CCATCAATTG 
AACGAAGTAG AAGAGCTCCT TAAGAAACAC AACCAATCAA GACAAACAGT TTTAGATAGT 
TTTAAAACTG TTTATGCTGA AAATATTCGT TTAATGGATA AAATTAAGCA ACAGGAACCT 
CAAAAATCTG GAGAGCAAGA TATGTATGTA GTTCGTTATT TGATGGAAAA TGAAAGAAAC 
CTTTGGGATC AATCATGTGA AACTTATTGT CAAAGAATCG AACAACAGCA ACAATTAAGT 
CAGTTTGACA ATGATCTCAT TCAGATCAAC AATAATTTAA ATGATCTTTC ACAACAACTG 
TCATCTACGC GAGGGCAATA TGGTGCAAAT TTAGCATCTG CCAAGTCAGC TTCACTTGCC 
TTTCATTATT TCGAAAAAAC TATATTACTT TTAGAACAAC GAATCGAAAC ATTCATTAAC 
GCAGCAAACT CATTGATGAA CTTTGATCAT AATAAGTCTG AACACATCCG AGAAGAATTA 
AATAAACTGA AGTCAAGATG GGATACGTTC CAAGCACAAG TGTTAGAAAG TCGTAACCAT 
ATTGATCTGT GTGTTCAATA TTTTACGTTA ATTAATGAAG CACAAGATTG GTTCCACGAA 
GGAAGTATAC TTCTTATGAA CATTGCTCGA AAATCAACCG AAGTCAAAAC AAGCGAAGAA 
GCTTCAGCAT TATTAAAAGA AATTGAAATA TTTTTAAAAC CTGGTGATAT AAGACAAGAT 
GAGAGATTAA ATCAAATACA CGAACTTAGC GTTCAACTTT TTGGAGAACA TGCAATAGCT 
GAAGTACCTC AAGTTGTTAC AGAAAATAAA GAGCTACTTG ATTCATTCTC ATTGATTGCA 
AAAGAATTAA AGTCTTTTGA CGATAAGCTT AAATCTAGAG AAAAATATGA AATAAATAAT 
GAAGTTAATC AAATAATTAC TGATACGTAC GAAGAAATAA TAACATCCAC ATCAATGACT 
AATGGCGTGT CACAAAATGA AGGTAATGTT CCATTATCAA AGAAAATAAA ACTCGAAGAA 
ATCCATAAAC CCAAACCTAC GATAATTCTA CCATTGCAAG ATAACACGGT TGATGAAGGG 
AAACAGTTTA CATTTGAATG CAGCATTGAA GGGGAACCTT CAAAAGTAAC CTGGTTCAAG 
GACAACATTT CCATCGACAA TAATCCAGAT TATCAGACAT CTAAAATTGA TAATCGATAT 
ATATTGAGAA TCGAGGAAAC GTTTGTAGAA GATTCAGCAA AATTTACATG TCGGGCCGAA 
AACGATTCTG ATTTTATTGA AACAAGTGCA TATCTTTCAG TTAAAGAATG TGCACCTGAA 
CACCAAATTA GTCCATCTGT ATTTACAAAA CTACTATCAG ATTTGCTAGT AAAAGAAGGC 
GATGCTTGTT CGTTATCTTG CAAAGCAGAA GGTAATCCAC TCCCAACAGT GCAGTGGTTT 
AAAGATGACA TTTGTATCGA TAATAGCCCT CAATACCAAA TTACATATAA TAATGGCGAA 
GCATTATTAA AAATTGAACA CACCAATTCG TCTACCCACA GTGGAAAATA TACTTGTGTT 
GCAACTAACA GACTTGGTTC AGATTCAACT GCGTCAAAGT TATTAATTGA TGCTCTGGAA 
AAGCCTGAAG ATGGGCCAAC GTTTGTTGTA CCTCTATCCA ATGTAATGGC TAGAGCCGGT 
CAAAAATTGA AATTGGAATG TGAAGTTGAA ACAAATAAGC CACCGATATT AATTTGGTTC 
CATAATGGGA AAGTCATGAA AGAAATAAAA GATTTCAAGG TTTCTGAAAA TCGTGGAAAA 
ATTAATTTAA TCATTCCGGA AGCCTTTCCC AAAGACGCTG GCACTTACAG TTTAACAATC 
AAAACGGAAC AGGGTGAAGC GACCAGTAGT TGTCAAGTAT CGGTGAAAGG TATACTCCCA 
AATGAAACTT CCGATTCGGA AATTGCAAGT GATTTGGAAC CGATCAAACC GTCAATACCG 
TTACTTTTAA AAGAACAAAC CGTTTTAGAA GGCAAAAGTG TTCATTTGAA ATGCATAATT 
GTTGGACAAC CCGAACCAGA GGTAATATGG TATCATGACG GAAGACCCGT TAAAGAATCC 
AAAGACATTC AACTTTTATT CCAAGGTGAT CAGTGTTCAC TTGTAATCAA GGAGGCTTTT 
ATCGAAGACG CGGGTGAATA CAAAGTAGTG GCTATTAACT CGGCAGGAGA AGCAAGCAGC 
ACATGCGTGT TAAGTGTCGA GCCTAAAGCA GTACCAGATA TAGACGAACT AGTAGTTTCA 
CCAAAGTTCA CTAAGCTGCT GTCGGACGTA TTAGTGAGGG AAGGTGATTC TATTACTCTA 
GAATGTAATG CCGAAGGTTG TCCGAAACCC GAATTTAAAT GGATTAGGAA CACTGTGGAA 
ATCAAGCCCG ACCAAAGGAT AACATTGAGT TCGGACGAAG ATGGTACGGC GACTCTTCAC 
ATACATAACG CTTTGTCCGT GGACAAAGGA CAGTACACCG TGAGAGCCGT GAATAAAGCC 
GGAGAAGCCA AATGTTTTTC GCACGTGATT GTAAAAGCCA TTTCGACGTT TGATACCGCG 
GTCAACCCAC CGACGGACAA AGTCCAATTC GAAGAAAAGT TGGAAAAACC ATCATTTACC 
GAGACGTTTC CGTCAGTCGT GAATGCATTC ACCGGCGAAA GTGTGAAATT CGAATGCATC 
GTTGTTGGCA AACCCGCTCC AAAGGTCCGT TGGCTTTTCA ACGAGAAGCC GGTGAGTGGA 
AAATCGTTTT TGGTGTCTAC CAGTGGCGAT CGTCAGGTAC TCACCATCCC CGAAGCGGAC 
ACCGGGCACG GCGGAATAGT CGAGTGCGTG GCCGAGAACG AAGCAGGCAA ATGCAAATGT 
GTGTCTTCGC TTAAAATAAA AGAAAGCGAA GCTGTTCCAT TAACCATGGC CTACAACGGC 
GGCGGTGATT TAAAACATAC GGTTTCCGAA GTTTATGAAT CCAGTTCGAA ATTCGAGTCG 
AAACAATCGA CGTTCGTGTC GTCATCGTCG TCGATCAGCG GTGACAACGC TCCTAAATTT 
AAATTGGACT CTTTCAGTTT ACAGTCGGAA AAACAAAGTA GGCAGTTTGG AGACAACGCT 
CCAATACAGT CTGAATCTTT ACGAACGGAA GAGTACCACA ACACGGACGG AAAGCAAAGT 
CAACATGTGA GTGATCTGAG TTCCACCAAC GTATCCGAAG AACATCAAAC TTCATTTTTC 
GAACGTGGAC TGTACAACCA AAGCGGGCTC GTTAAGGACG CCTCACAAAA ATCTTTGCTC 
GAAAGAAACA TTTCGAAACA ATCAAGGAAA GAAATGGCAC CGCGTTTCGT GTCGGCTCCA 
CAAGGAAAAA TCGCTGAACA GGGAAAAGAC GTATTTTTGG ATGCTATAAT AGACAGTTAT 
CCGCCATCTG ACATCACGTG GAGCAAAAAC GGTATCGACT TGGTTCCCGA CGGTGTGAAA 
TTGAAAATAA CCAGCGAAGT CAACAAAACT CGGTTGGACA TTAAACGTCT AACTGTGGAA 
GACGGTGGCC AATATACGTG CAAGGCTATG AACACCGCTG GGGGCACGAG TTGTACAACA 
GACGTCATTG TTAAAAAAAA CGTATTTCCA CCGGTCATGT GTCGAAGACT TTTACCGAAC 
ACGGTCGCTG AAGGCGAAAG AGTAGTTTTA GAAATTGAAG TGGCCGGTAC GCCCGAACCA 
ACAGTGAGTT GGTTTAAAAA TAATCAACCA CTATCGGCCA CGTCGAGTTT ATACAGATTA 
AGACAACAGG GGTCTTGTAG TGCCGTAGTT ATCGATAAAG CTACTACAGA ACATGCTGGA 
GAGTACAAAG TTATGGCGAA AAACGAAGGC GGGGAGGCTG TAAGTTGTGC CGATTTGAGA 
GTGGTTCAGC CTTTACCCGA TATCGGATTA AATTTGTTAC CCACTTATGC ACCGAATGAC 
AACACGGATA AAGTGAATGT GGATTACTCC AAAAGTATTG TACCGACAAA TCAATCGAAG 
CAATTTAATA TTACTAGCAA AACAGAAGAG AAATCACATT TTTCTAAATC TGTCTTCGTT 
CAAGAATCGA TAAACAGCAG TAAGGATGTA ATTATGACTG TTAACCGCTC TGAAGCAAAT 
GACTTTGATT TAAATTCGAA TAATGCAAAC GCTAATTATG AAAATACTGA AAAACAGATA 
TCTGTTCAAA ACGAAAATAT CGAAAATGCA TCGATAACGA AAAAAAGTGC ACTCCAGTTC 
TTTAAAAATG TCATTGAAGA TAATAAAGTT GAAAAGTCTA GTAATTCAAC TATTCCGCCA 
TCAGCAGGAA TTTATAAAAG AGAAAGTCCC ATAGATAATT CAAGTAATTT TGCATCCGAT 
GTTCATAATA AGACGTATAA TATTGAAGAA AACGTATCAA GTAGCTCATT TAAACAGTCA 
AACGACACGG AAGTCATTTT AGAACCAGGT CCACCGCCCA CTTTTGATTA TATGCCAAGA 
GCGCAATCTG TAAAAAAAGA TCAAATGACC GAACGGTTGA GAAGACTATC AACAAATCAA 
AAATTATTAT CACCCGAACA AATTCCGTCC GGCGCTGTTA GAATATTTCC TGACGTAACC 
TCTGAGATAA AACAAGAAGT TGTTGAGGAA AGTTGTATAA AAAAAGAAGT TATTAAATCT 
GGGTTATCCG AAGACACAAT AGCACACACA CATTTGCCTA CATCCTATAC GCCACTGCCA 
CACAGTGAAC CTCATGTTCC ACTTTTGAGA CCCCAAGCCG ACATAGAAGT GAGACCAGGA 
TCTCCAAGAC CTTCTGCAGA AGCTATTTCT ATGGAAAAGC TATGGACAAA AGGGCATACT 
CCACATGTTC AAAAAACTCC AGTATCGTAT TCACCGATTC ATATGAAAAC ATATTCATCG 
ACGGAAAGCC AATCGTTTGT TTCAGAGACC ATTGAAAAAA ATGGTGAACT GGTAAAAAAT 
GAAAGCAGAG AAGAGAAACA AGGTAGTCTA AAAATAGACG ATGGTGTTAA AAAAGTTTCA 
GAATCGTTTT CAGAAATATC AATTCGTCCG ACGAAACACG TCGAACCACC ACGAAATGAA 
ATTAAACGCC CAGCATCAGC ACATTTGTTT AATGAAGTAA AGTCTAAAGA TGCGTTCTTC 
GGCAACAAAG TCCAAACACA ACATTTTTCT GAAGAATCGT CACAAATTAA AAAAAGTTCA 
ATGTTTGACG TGAAATCAAA TATTTCTGAA AAAGTAGAAA GAAACAGTTC CAATGTAAAA 
TCTGAACATC AATTGCAAAA TAATCAATCG TTTGATAGAA ATATGCAAAA CACATATCAA 
TTTTTACCAG AACGTTCAAT AGGTCCAATA AAACACGTGG AACCACCGAA CAACAGCAAG 
ATAATTTCTA ATTTTACACG GTCACACAGC GTAGAAACTG CCAAGGAAAA GCAATGGTTG 
TCTCAATCAC CGGTAGCTTT TAGAGGACCG CCTCAAAATA AAACGTATTC ATCGTTTGAA 
GCGCATTCAA GTTTTTCAAA AACCTTAGAA AAGGACGGAG TGTTAGTAGC CTCCGAACAT 
AACGAAGAAG GCGGTCGAGT AGTGGACGAC GGCGTTCAAA AAGCATATGA TTCATTTTCA 
GAACGTTCAA AATTACCGAC AAAGTTTGTG GACCTTTCAA AAAATACTAA TAAATCAAAT 
TCTATAAAAA CCATGCAAAA AATGTTCGAG CAGACTGGTT CGTCGTCTAC GGTCTCTAAT 
GTTGGTTCAA ACGTAAGACC ATACAGTAGA ATTAAGTCCC GAGCAAGTGA TAGCGATTTC 
GAAAGTGAAG CGGAATTGTC AAAATACAAC GTAGAGCAAA AAGTTTCAGA AAGTTTTAAC 
AGTTTTGAAA CGAAAATGTA TTCTTCAAAA ACTCAATTAC AAGAAATTAA AAACACTTCG 
ATAATCATGC AAAGTCCGAT TAAGGAAAGT GGTTACGCGG CTGACACGGA CGAGCCAAGG 
GGATTTCAGA GCGAATCGGT AGTTAATAAT CAAACGTTTA ACTCCAAAAG GAATTCGGGG 
TTTCAAAGCA CTTCAATAAA GAAGGCTGAG TTTTTGGAAA ACGAACGCCG TCAACCGCAC 
CGGGCACATC CGACGCAGTG GGCAAACCAG TACAATCAAC ACAGTCGGAC TCAAGTCCGG 
AGCCCCGCGA AGTTCGTCAG GGGTGAATTC CGAGAGAGCG ATTACGAGAG CGACTACGAG 
TGCAGGATAC AACCAATATG GCGCCCGTAC GATTCGGAAT CGGAGGAACC GGTGTACAAG 
CCGGTCAGGC CTGGTTTCAA GACTCCCGTA AAAAAGCCCG AGGGGTCGCG ACTCTTGCAG 
CCAGTGCAGC GATCGGCGGT GCCGCCGGTG GTGGCTTTCG AACTGAAACC AGGGTCACCG 
CCCGAAATGG GTTTCGCGCC GTCGCCGGAC GTGCATAGGT CGGTGAGCTT CGTCGAGTCC 
GAACGCGACG AAAGCGTGCA GAGGACCACA CACTTCTTGT CGTCCGACGG TCGCAGTCAC 
GGGACGCTGG AGCGAAATGC CGAGGCGAAG CGGTTACAAA GGGTAGACGA GATGAGGAAG 
AGGTTCGAAC AAAAGTCACT GTGTCCGGCC GAATCGAGTT CGGTGCAACA GACGGTCTCG 
TCTCAACTCG GGCAAGGTAC TAACCAAATC GATTTGTCCC AAACCGGAAT TCCGTATTTC 
AAACACGATT TCGGATATGA GTTCGGTGTA GTGTATCCCG TGAACACGAG AAACGGTAAA 
ACGGCTAACC CGACGGATAG CGTCAGCATA CCAGTGATAC ACGAGTTTTC CAAATCCGTT 
CGGACACAGA CATCCGAGAT GTCTAATTTT AACACACGCC GAGAACATTT TAAGACAACA 
ACAGATGGAA CCGTTATTAG CCCCGTTTAC CAAAAAGAAC TGAACGCCGC CGAACACTCG 
GATCCATCGC ACAGTTCACC TTTCTTCGTA ACCCCGTTAA AAGACATAGC TGTAGTTAGC 
GGCAAGACTG CCAGATTCGA GTGCATAGTG CAAGGTGAAC CCGTCAGTGA AATCGTGTGG 
TCGTGTAACG GCCAAGTGCT GGAGAACTCG GAGTGCTACC AGGCGCAGTA CAGGAACGGC 
GTGTGCAGAC TGACGATACC CAGGACGTAC CAATTCAATG CCGGATCGTA CTCGTGCACC 
GTATCCAATT ACCTGGGGTC CAGCAGCACG TCGGCCGACC TCCGAGTGTC AGGTGAGTCT 
CGCACGTTCA TTCAACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029342920.1
CDS	195..9875
Translation	MDRDSRPPATTVSTIAVRSGATRIVIALLQCRDVLELKVLELCPELTLLGETLEEASSLQAEHERVLEKIQEKQSPVNELLKKADYLIATQNTQPQVYAAMADSLGLAWKDVNDVLQKRSYLLHLNVEYHRQAEVCMERIGTLEMMCHSYLPTDVESIKNHLEHIQEVRKSILEALMVALGNGTQLLDVLKEMQAKGTLDSRPGYNYHSISLAVSQVEHWLEKLHDKRHSLDIDCHERKTMLEKRLTISLLVTDLNLLENILSERRNSLERVKNCLGDSQYECSKFTEVNSNLITEAKDIRDQALRVARATEQLVLTKGYMEEDYKFKAYKLLELATEYLEELNRRETLLDAAMKFFESAEKVLRAWETILQHSSDEQLASKNTYVTETVKRTGASVIQEGNLILKDLPEAEGVKRVVDELERKKQLILSIVMKDRDKYMESSEAVNTFSENYNKIFSWLVSLAESFLHEHNSMGTNLTEAREFLISHKTLQRDLRTRGIDINALVNSTSLPPLTYIDAETRVDLEGKSIVLKDHWTYLDRILEWRIQLSELYVKFYTSCEHLNIEFSNLDSIIGDHVDVSDQRLDSAHKYWTNILQLYIQLKNTGEKFLHQSSKSEDAHLELDVARDRVRSLLDTFVDRKTRTTSVWETWQRASAENKLDKVLWSETMCESSKTVDWVSKLESQLYPVLKSEPSSVDRHISEVKQKLNNVLPEVQRAQDDIESRIKTAEELIGKGNIQGDNISIPPRLKELHSRLVGITTEYQNLLDMFLSFFNSLQEFHDIVKESERGRPTDIFNHQLNEVEELLKKHNQSRQTVLDSFKTVYAENIRLMDKIKQQEPQKSGEQDMYVVRYLMENERNLWDQSCETYCQRIEQQQQLSQFDNDLIQINNNLNDLSQQLSSTRGQYGANLASAKSASLAFHYFEKTILLLEQRIETFINAANSLMNFDHNKSEHIREELNKLKSRWDTFQAQVLESRNHIDLCVQYFTLINEAQDWFHEGSILLMNIARKSTEVKTSEEASALLKEIEIFLKPGDIRQDERLNQIHELSVQLFGEHAIAEVPQVVTENKELLDSFSLIAKELKSFDDKLKSREKYEINNEVNQIITDTYEEIITSTSMTNGVSQNEGNVPLSKKIKLEEIHKPKPTIILPLQDNTVDEGKQFTFECSIEGEPSKVTWFKDNISIDNNPDYQTSKIDNRYILRIEETFVEDSAKFTCRAENDSDFIETSAYLSVKECAPEHQISPSVFTKLLSDLLVKEGDACSLSCKAEGNPLPTVQWFKDDICIDNSPQYQITYNNGEALLKIEHTNSSTHSGKYTCVATNRLGSDSTASKLLIDALEKPEDGPTFVVPLSNVMARAGQKLKLECEVETNKPPILIWFHNGKVMKEIKDFKVSENRGKINLIIPEAFPKDAGTYSLTIKTEQGEATSSCQVSVKGILPNETSDSEIASDLEPIKPSIPLLLKEQTVLEGKSVHLKCIIVGQPEPEVIWYHDGRPVKESKDIQLLFQGDQCSLVIKEAFIEDAGEYKVVAINSAGEASSTCVLSVEPKAVPDIDELVVSPKFTKLLSDVLVREGDSITLECNAEGCPKPEFKWIRNTVEIKPDQRITLSSDEDGTATLHIHNALSVDKGQYTVRAVNKAGEAKCFSHVIVKAISTFDTAVNPPTDKVQFEEKLEKPSFTETFPSVVNAFTGESVKFECIVVGKPAPKVRWLFNEKPVSGKSFLVSTSGDRQVLTIPEADTGHGGIVECVAENEAGKCKCVSSLKIKESEAVPLTMAYNGGGDLKHTVSEVYESSSKFESKQSTFVSSSSSISGDNAPKFKLDSFSLQSEKQSRQFGDNAPIQSESLRTEEYHNTDGKQSQHVSDLSSTNVSEEHQTSFFERGLYNQSGLVKDASQKSLLERNISKQSRKEMAPRFVSAPQGKIAEQGKDVFLDAIIDSYPPSDITWSKNGIDLVPDGVKLKITSEVNKTRLDIKRLTVEDGGQYTCKAMNTAGGTSCTTDVIVKKNVFPPVMCRRLLPNTVAEGERVVLEIEVAGTPEPTVSWFKNNQPLSATSSLYRLRQQGSCSAVVIDKATTEHAGEYKVMAKNEGGEAVSCADLRVVQPLPDIGLNLLPTYAPNDNTDKVNVDYSKSIVPTNQSKQFNITSKTEEKSHFSKSVFVQESINSSKDVIMTVNRSEANDFDLNSNNANANYENTEKQISVQNENIENASITKKSALQFFKNVIEDNKVEKSSNSTIPPSAGIYKRESPIDNSSNFASDVHNKTYNIEENVSSSSFKQSNDTEVILEPGPPPTFDYMPRAQSVKKDQMTERLRRLSTNQKLLSPEQIPSGAVRIFPDVTSEIKQEVVEESCIKKEVIKSGLSEDTIAHTHLPTSYTPLPHSEPHVPLLRPQADIEVRPGSPRPSAEAISMEKLWTKGHTPHVQKTPVSYSPIHMKTYSSTESQSFVSETIEKNGELVKNESREEKQGSLKIDDGVKKVSESFSEISIRPTKHVEPPRNEIKRPASAHLFNEVKSKDAFFGNKVQTQHFSEESSQIKKSSMFDVKSNISEKVERNSSNVKSEHQLQNNQSFDRNMQNTYQFLPERSIGPIKHVEPPNNSKIISNFTRSHSVETAKEKQWLSQSPVAFRGPPQNKTYSSFEAHSSFSKTLEKDGVLVASEHNEEGGRVVDDGVQKAYDSFSERSKLPTKFVDLSKNTNKSNSIKTMQKMFEQTGSSSTVSNVGSNVRPYSRIKSRASDSDFESEAELSKYNVEQKVSESFNSFETKMYSSKTQLQEIKNTSIIMQSPIKESGYAADTDEPRGFQSESVVNNQTFNSKRNSGFQSTSIKKAEFLENERRQPHRAHPTQWANQYNQHSRTQVRSPAKFVRGEFRESDYESDYECRIQPIWRPYDSESEEPVYKPVRPGFKTPVKKPEGSRLLQPVQRSAVPPVVAFELKPGSPPEMGFAPSPDVHRSVSFVESERDESVQRTTHFLSSDGRSHGTLERNAEAKRLQRVDEMRKRFEQKSLCPAESSSVQQTVSSQLGQGTNQIDLSQTGIPYFKHDFGYEFGVVYPVNTRNGKTANPTDSVSIPVIHEFSKSVRTQTSEMSNFNTRREHFKTTTDGTVISPVYQKELNAAEHSDPSHSSPFFVTPLKDIAVVSGKTARFECIVQGEPVSEIVWSCNGQVLENSECYQAQYRNGVCRLTIPRTYQFNAGSYSCTVSNYLGSSSTSADLRVSGESRTFIQH

Target ORF information:

RefSeq Version	XM_029487060.1
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029487060.1

ORF Insert Sequence:

ATGGACCGCG ACTCCCGGCC GCCGGCCACC ACCGTCAGCA CAATAGCCGT CCGAAGTGGC 
GCCACCAGGA TCGTCATAGC CCTATTACAG TGTCGTGACG TTTTAGAATT AAAAGTTCTC 
GAACTATGCC CGGAACTTAC GTTGCTCGGG GAGACGTTGG AAGAGGCCTC GAGCTTACAG 
GCCGAACACG AGAGAGTGTT GGAAAAAATA CAGGAAAAGC AGAGTCCCGT CAACGAGTTG 
TTGAAGAAAG CCGATTATCT AATAGCTACA CAGAACACGC AGCCCCAGGT GTATGCCGCT 
ATGGCCGATT CGCTGGGATT AGCCTGGAAA GACGTAAACG ATGTACTTCA AAAGAGAAGT 
TACTTGTTAC ACCTGAACGT TGAATATCAC CGACAAGCTG AAGTATGCAT GGAACGAATC 
GGAACTTTGG AAATGATGTG CCACAGTTAC CTGCCGACCG ACGTCGAATC GATCAAAAAC 
CATCTGGAGC ATATCCAGGA AGTCCGCAAG TCTATACTGG AAGCGCTAAT GGTCGCTTTG 
GGCAATGGCA CGCAGCTACT GGACGTTTTG AAAGAGATGC AAGCCAAAGG AACCCTGGAT 
TCGAGGCCGG GATATAATTA TCATTCTATT TCATTAGCCG TGTCTCAAGT AGAACATTGG 
TTGGAGAAAC TTCACGATAA ACGTCATTCG TTGGACATTG ACTGTCACGA ACGTAAGACA 
ATGTTGGAAA AACGACTGAC AATATCTTTA CTAGTCACCG ATCTAAATTT ACTTGAAAAC 
ATACTATCTG AAAGAAGAAA TTCTCTGGAA AGAGTTAAAA ATTGTCTAGG CGACTCCCAG 
TATGAATGTT CTAAATTTAC GGAAGTCAAT TCGAACTTGA TAACCGAAGC AAAGGATATA 
AGAGACCAAG CGCTGAGGGT TGCAAGAGCC ACCGAACAAC TGGTATTAAC TAAAGGCTAC 
ATGGAAGAGG ATTATAAATT TAAAGCGTAT AAACTATTAG AATTAGCTAC CGAATACTTG 
GAAGAACTAA ATAGAAGAGA AACTCTTTTA GATGCCGCTA TGAAATTTTT CGAGTCGGCT 
GAAAAAGTTC TCCGAGCATG GGAAACCATA CTTCAGCATT CATCCGATGA GCAATTGGCA 
TCAAAGAACA CGTACGTTAC AGAGACGGTA AAGCGAACCG GCGCCTCAGT AATTCAAGAA 
GGAAATTTAA TACTGAAAGA CTTACCAGAG GCAGAGGGCG TTAAACGGGT TGTAGACGAG 
TTGGAAAGGA AAAAACAGCT AATACTGTCA ATTGTTATGA AAGACCGCGA CAAATATATG 
GAATCTTCAG AAGCAGTCAA CACGTTTTCA GAGAATTATA ATAAAATATT TTCCTGGCTT 
GTAAGTTTAG CTGAATCGTT TTTACACGAA CATAATTCTA TGGGTACAAA TCTCACGGAA 
GCGCGAGAGT TTTTAATATC CCATAAAACT TTACAAAGAG ATCTAAGGAC TCGAGGGATC 
GACATTAACG CATTGGTAAA CTCGACTAGT CTGCCACCGT TAACGTACAT CGATGCTGAA 
ACAAGAGTCG ATCTGGAAGG CAAATCAATC GTGTTAAAAG ACCATTGGAC TTATTTGGAT 
AGAATATTGG AATGGAGGAT ACAATTGTCC GAGTTGTATG TCAAATTTTA CACGTCGTGT 
GAACATTTGA ACATCGAATT TTCAAACTTG GACTCGATCA TTGGAGATCA CGTCGACGTG 
TCTGATCAAA GACTAGATTC GGCGCACAAG TATTGGACAA ACATTTTACA ATTGTATATC 
CAACTTAAGA ACACAGGAGA AAAGTTTTTG CATCAAAGTT CAAAATCGGA GGACGCTCAT 
CTGGAACTAG ACGTAGCACG GGACCGCGTG CGATCGCTAC TGGACACGTT CGTGGACAGG 
AAAACGAGAA CTACTTCCGT CTGGGAGACT TGGCAGCGGG CATCGGCTGA AAACAAACTG 
GATAAAGTGC TCTGGTCGGA GACGATGTGC GAGAGCAGCA AGACAGTAGA TTGGGTTTCC 
AAATTGGAAT CTCAATTATA TCCAGTTTTG AAATCTGAGC CGTCATCAGT TGATCGACAT 
ATTTCAGAGG TTAAACAGAA GTTAAATAAT GTTCTACCGG AAGTTCAAAG GGCTCAAGAC 
GATATCGAGT CCAGAATAAA AACAGCCGAA GAACTTATTG GAAAAGGAAA TATTCAAGGT 
GATAATATTT CTATACCTCC AAGGTTAAAA GAACTTCATA GCCGTCTTGT TGGAATCACT 
ACAGAGTATC AAAATCTTTT AGATATGTTC TTATCATTTT TTAATTCTCT ACAAGAATTT 
CACGATATTG TGAAAGAATC TGAAAGAGGT AGGCCCACCG ACATTTTCAA CCATCAATTG 
AACGAAGTAG AAGAGCTCCT TAAGAAACAC AACCAATCAA GACAAACAGT TTTAGATAGT 
TTTAAAACTG TTTATGCTGA AAATATTCGT TTAATGGATA AAATTAAGCA ACAGGAACCT 
CAAAAATCTG GAGAGCAAGA TATGTATGTA GTTCGTTATT TGATGGAAAA TGAAAGAAAC 
CTTTGGGATC AATCATGTGA AACTTATTGT CAAAGAATCG AACAACAGCA ACAATTAAGT 
CAGTTTGACA ATGATCTCAT TCAGATCAAC AATAATTTAA ATGATCTTTC ACAACAACTG 
TCATCTACGC GAGGGCAATA TGGTGCAAAT TTAGCATCTG CCAAGTCAGC TTCACTTGCC 
TTTCATTATT TCGAAAAAAC TATATTACTT TTAGAACAAC GAATCGAAAC ATTCATTAAC 
GCAGCAAACT CATTGATGAA CTTTGATCAT AATAAGTCTG AACACATCCG AGAAGAATTA 
AATAAACTGA AGTCAAGATG GGATACGTTC CAAGCACAAG TGTTAGAAAG TCGTAACCAT 
ATTGATCTGT GTGTTCAATA TTTTACGTTA ATTAATGAAG CACAAGATTG GTTCCACGAA 
GGAAGTATAC TTCTTATGAA CATTGCTCGA AAATCAACCG AAGTCAAAAC AAGCGAAGAA 
GCTTCAGCAT TATTAAAAGA AATTGAAATA TTTTTAAAAC CTGGTGATAT AAGACAAGAT 
GAGAGATTAA ATCAAATACA CGAACTTAGC GTTCAACTTT TTGGAGAACA TGCAATAGCT 
GAAGTACCTC AAGTTGTTAC AGAAAATAAA GAGCTACTTG ATTCATTCTC ATTGATTGCA 
AAAGAATTAA AGTCTTTTGA CGATAAGCTT AAATCTAGAG AAAAATATGA AATAAATAAT 
GAAGTTAATC AAATAATTAC TGATACGTAC GAAGAAATAA TAACATCCAC ATCAATGACT 
AATGGCGTGT CACAAAATGA AGGTAATGTT CCATTATCAA AGAAAATAAA ACTCGAAGAA 
ATCCATAAAC CCAAACCTAC GATAATTCTA CCATTGCAAG ATAACACGGT TGATGAAGGG 
AAACAGTTTA CATTTGAATG CAGCATTGAA GGGGAACCTT CAAAAGTAAC CTGGTTCAAG 
GACAACATTT CCATCGACAA TAATCCAGAT TATCAGACAT CTAAAATTGA TAATCGATAT 
ATATTGAGAA TCGAGGAAAC GTTTGTAGAA GATTCAGCAA AATTTACATG TCGGGCCGAA 
AACGATTCTG ATTTTATTGA AACAAGTGCA TATCTTTCAG TTAAAGAATG TGCACCTGAA 
CACCAAATTA GTCCATCTGT ATTTACAAAA CTACTATCAG ATTTGCTAGT AAAAGAAGGC 
GATGCTTGTT CGTTATCTTG CAAAGCAGAA GGTAATCCAC TCCCAACAGT GCAGTGGTTT 
AAAGATGACA TTTGTATCGA TAATAGCCCT CAATACCAAA TTACATATAA TAATGGCGAA 
GCATTATTAA AAATTGAACA CACCAATTCG TCTACCCACA GTGGAAAATA TACTTGTGTT 
GCAACTAACA GACTTGGTTC AGATTCAACT GCGTCAAAGT TATTAATTGA TGCTCTGGAA 
AAGCCTGAAG ATGGGCCAAC GTTTGTTGTA CCTCTATCCA ATGTAATGGC TAGAGCCGGT 
CAAAAATTGA AATTGGAATG TGAAGTTGAA ACAAATAAGC CACCGATATT AATTTGGTTC 
CATAATGGGA AAGTCATGAA AGAAATAAAA GATTTCAAGG TTTCTGAAAA TCGTGGAAAA 
ATTAATTTAA TCATTCCGGA AGCCTTTCCC AAAGACGCTG GCACTTACAG TTTAACAATC 
AAAACGGAAC AGGGTGAAGC GACCAGTAGT TGTCAAGTAT CGGTGAAAGG TATACTCCCA 
AATGAAACTT CCGATTCGGA AATTGCAAGT GATTTGGAAC CGATCAAACC GTCAATACCG 
TTACTTTTAA AAGAACAAAC CGTTTTAGAA GGCAAAAGTG TTCATTTGAA ATGCATAATT 
GTTGGACAAC CCGAACCAGA GGTAATATGG TATCATGACG GAAGACCCGT TAAAGAATCC 
AAAGACATTC AACTTTTATT CCAAGGTGAT CAGTGTTCAC TTGTAATCAA GGAGGCTTTT 
ATCGAAGACG CGGGTGAATA CAAAGTAGTG GCTATTAACT CGGCAGGAGA AGCAAGCAGC 
ACATGCGTGT TAAGTGTCGA GCCTAAAGCA GTACCAGATA TAGACGAACT AGTAGTTTCA 
CCAAAGTTCA CTAAGCTGCT GTCGGACGTA TTAGTGAGGG AAGGTGATTC TATTACTCTA 
GAATGTAATG CCGAAGGTTG TCCGAAACCC GAATTTAAAT GGATTAGGAA CACTGTGGAA 
ATCAAGCCCG ACCAAAGGAT AACATTGAGT TCGGACGAAG ATGGTACGGC GACTCTTCAC 
ATACATAACG CTTTGTCCGT GGACAAAGGA CAGTACACCG TGAGAGCCGT GAATAAAGCC 
GGAGAAGCCA AATGTTTTTC GCACGTGATT GTAAAAGCCA TTTCGACGTT TGATACCGCG 
GTCAACCCAC CGACGGACAA AGTCCAATTC GAAGAAAAGT TGGAAAAACC ATCATTTACC 
GAGACGTTTC CGTCAGTCGT GAATGCATTC ACCGGCGAAA GTGTGAAATT CGAATGCATC 
GTTGTTGGCA AACCCGCTCC AAAGGTCCGT TGGCTTTTCA ACGAGAAGCC GGTGAGTGGA 
AAATCGTTTT TGGTGTCTAC CAGTGGCGAT CGTCAGGTAC TCACCATCCC CGAAGCGGAC 
ACCGGGCACG GCGGAATAGT CGAGTGCGTG GCCGAGAACG AAGCAGGCAA ATGCAAATGT 
GTGTCTTCGC TTAAAATAAA AGAAAGCGAA GCTGTTCCAT TAACCATGGC CTACAACGGC 
GGCGGTGATT TAAAACATAC GGTTTCCGAA GTTTATGAAT CCAGTTCGAA ATTCGAGTCG 
AAACAATCGA CGTTCGTGTC GTCATCGTCG TCGATCAGCG GTGACAACGC TCCTAAATTT 
AAATTGGACT CTTTCAGTTT ACAGTCGGAA AAACAAAGTA GGCAGTTTGG AGACAACGCT 
CCAATACAGT CTGAATCTTT ACGAACGGAA GAGTACCACA ACACGGACGG AAAGCAAAGT 
CAACATGTGA GTGATCTGAG TTCCACCAAC GTATCCGAAG AACATCAAAC TTCATTTTTC 
GAACGTGGAC TGTACAACCA AAGCGGGCTC GTTAAGGACG CCTCACAAAA ATCTTTGCTC 
GAAAGAAACA TTTCGAAACA ATCAAGGAAA GAAATGGCAC CGCGTTTCGT GTCGGCTCCA 
CAAGGAAAAA TCGCTGAACA GGGAAAAGAC GTATTTTTGG ATGCTATAAT AGACAGTTAT 
CCGCCATCTG ACATCACGTG GAGCAAAAAC GGTATCGACT TGGTTCCCGA CGGTGTGAAA 
TTGAAAATAA CCAGCGAAGT CAACAAAACT CGGTTGGACA TTAAACGTCT AACTGTGGAA 
GACGGTGGCC AATATACGTG CAAGGCTATG AACACCGCTG GGGGCACGAG TTGTACAACA 
GACGTCATTG TTAAAAAAAA CGTATTTCCA CCGGTCATGT GTCGAAGACT TTTACCGAAC 
ACGGTCGCTG AAGGCGAAAG AGTAGTTTTA GAAATTGAAG TGGCCGGTAC GCCCGAACCA 
ACAGTGAGTT GGTTTAAAAA TAATCAACCA CTATCGGCCA CGTCGAGTTT ATACAGATTA 
AGACAACAGG GGTCTTGTAG TGCCGTAGTT ATCGATAAAG CTACTACAGA ACATGCTGGA 
GAGTACAAAG TTATGGCGAA AAACGAAGGC GGGGAGGCTG TAAGTTGTGC CGATTTGAGA 
GTGGTTCAGC CTTTACCCGA TATCGGATTA AATTTGTTAC CCACTTATGC ACCGAATGAC 
AACACGGATA AAGTGAATGT GGATTACTCC AAAAGTATTG TACCGACAAA TCAATCGAAG 
CAATTTAATA TTACTAGCAA AACAGAAGAG AAATCACATT TTTCTAAATC TGTCTTCGTT 
CAAGAATCGA TAAACAGCAG TAAGGATGTA ATTATGACTG TTAACCGCTC TGAAGCAAAT 
GACTTTGATT TAAATTCGAA TAATGCAAAC GCTAATTATG AAAATACTGA AAAACAGATA 
TCTGTTCAAA ACGAAAATAT CGAAAATGCA TCGATAACGA AAAAAAGTGC ACTCCAGTTC 
TTTAAAAATG TCATTGAAGA TAATAAAGTT GAAAAGTCTA GTAATTCAAC TATTCCGCCA 
TCAGCAGGAA TTTATAAAAG AGAAAGTCCC ATAGATAATT CAAGTAATTT TGCATCCGAT 
GTTCATAATA AGACGTATAA TATTGAAGAA AACGTATCAA GTAGCTCATT TAAACAGTCA 
AACGACACGG AAGTCATTTT AGAACCAGGT CCACCGCCCA CTTTTGATTA TATGCCAAGA 
GCGCAATCTG TAAAAAAAGA TCAAATGACC GAACGGTTGA GAAGACTATC AACAAATCAA 
AAATTATTAT CACCCGAACA AATTCCGTCC GGCGCTGTTA GAATATTTCC TGACGTAACC 
TCTGAGATAA AACAAGAAGT TGTTGAGGAA AGTTGTATAA AAAAAGAAGT TATTAAATCT 
GGGTTATCCG AAGACACAAT AGCACACACA CATTTGCCTA CATCCTATAC GCCACTGCCA 
CACAGTGAAC CTCATGTTCC ACTTTTGAGA CCCCAAGCCG ACATAGAAGT GAGACCAGGA 
TCTCCAAGAC CTTCTGCAGA AGCTATTTCT ATGGAAAAGC TATGGACAAA AGGGCATACT 
CCACATGTTC AAAAAACTCC AGTATCGTAT TCACCGATTC ATATGAAAAC ATATTCATCG 
ACGGAAAGCC AATCGTTTGT TTCAGAGACC ATTGAAAAAA ATGGTGAACT GGTAAAAAAT 
GAAAGCAGAG AAGAGAAACA AGGTAGTCTA AAAATAGACG ATGGTGTTAA AAAAGTTTCA 
GAATCGTTTT CAGAAATATC AATTCGTCCG ACGAAACACG TCGAACCACC ACGAAATGAA 
ATTAAACGCC CAGCATCAGC ACATTTGTTT AATGAAGTAA AGTCTAAAGA TGCGTTCTTC 
GGCAACAAAG TCCAAACACA ACATTTTTCT GAAGAATCGT CACAAATTAA AAAAAGTTCA 
ATGTTTGACG TGAAATCAAA TATTTCTGAA AAAGTAGAAA GAAACAGTTC CAATGTAAAA 
TCTGAACATC AATTGCAAAA TAATCAATCG TTTGATAGAA ATATGCAAAA CACATATCAA 
TTTTTACCAG AACGTTCAAT AGGTCCAATA AAACACGTGG AACCACCGAA CAACAGCAAG 
ATAATTTCTA ATTTTACACG GTCACACAGC GTAGAAACTG CCAAGGAAAA GCAATGGTTG 
TCTCAATCAC CGGTAGCTTT TAGAGGACCG CCTCAAAATA AAACGTATTC ATCGTTTGAA 
GCGCATTCAA GTTTTTCAAA AACCTTAGAA AAGGACGGAG TGTTAGTAGC CTCCGAACAT 
AACGAAGAAG GCGGTCGAGT AGTGGACGAC GGCGTTCAAA AAGCATATGA TTCATTTTCA 
GAACGTTCAA AATTACCGAC AAAGTTTGTG GACCTTTCAA AAAATACTAA TAAATCAAAT 
TCTATAAAAA CCATGCAAAA AATGTTCGAG CAGACTGGTT CGTCGTCTAC GGTCTCTAAT 
GTTGGTTCAA ACGTAAGACC ATACAGTAGA ATTAAGTCCC GAGCAAGTGA TAGCGATTTC 
GAAAGTGAAG CGGAATTGTC AAAATACAAC GTAGAGCAAA AAGTTTCAGA AAGTTTTAAC 
AGTTTTGAAA CGAAAATGTA TTCTTCAAAA ACTCAATTAC AAGAAATTAA AAACACTTCG 
ATAATCATGC AAAGTCCGAT TAAGGAAAGT GGTTACGCGG CTGACACGGA CGAGCCAAGG 
GGATTTCAGA GCGAATCGGT AGTTAATAAT CAAACGTTTA ACTCCAAAAG GAATTCGGGG 
TTTCAAAGCA CTTCAATAAA GAAGGCTGAG TTTTTGGAAA ACGAACGCCG TCAACCGCAC 
CGGGCACATC CGACGCAGTG GGCAAACCAG TACAATCAAC ACAGTCGGAC TCAAGTCCGG 
AGCCCCGCGA AGTTCGTCAG GGGTGAATTC CGAGAGAGCG ATTACGAGAG CGACTACGAG 
TGCAGGATAC AACCAATATG GCGCCCGTAC GATTCGGAAT CGGAGGAACC GGTGTACAAG 
CCGGTCAGGC CTGGTTTCAA GACTCCCGTA AAAAAGCCCG AGGGGTCGCG ACTCTTGCAG 
CCAGTGCAGC GATCGGCGGT GCCGCCGGTG GTGGCTTTCG AACTGAAACC AGGGTCACCG 
CCCGAAATGG GTTTCGCGCC GTCGCCGGAC GTGCATAGGT CGGTGAGCTT CGTCGAGTCC 
GAACGCGACG AAAGCGTGCA GAGGACCACA CACTTCTTGT CGTCCGACGG TCGCAGTCAC 
GGGACGCTGG AGCGAAATGC CGAGGCGAAG CGGTTACAAA GGGTAGACGA GATGAGGAAG 
AGGTTCGAAC AAAAGTCACT GTGTCCGGCC GAATCGAGTT CGGTGCAACA GACGGTCTCG 
TCTCAACTCG GGCAAGGTAC TAACCAAATC GATTTGTCCC AAACCGGAAT TCCGTATTTC 
AAACACGATT TCGGATATGA GTTCGGTGTA GTGTATCCCG TGAACACGAG AAACGGTAAA 
ACGGCTAACC CGACGGATAG CGTCAGCATA CCAGTGATAC ACGAGTTTTC CAAATCCGTT 
CGGACACAGA CATCCGAGAT GTCTAATTTT AACACACGCC GAGAACATTT TAAGACAACA 
ACAGATGGAA CCGTTATTAG CCCCGTTTAC CAAAAAGAAC TGAACGCCGC CGAACACTCG 
GATCCATCGC ACAGTTCACC TTTCTTCGTA ACCCCGTTAA AAGACATAGC TGTAGTTAGC 
GGCAAGACTG CCAGATTCGA GTGCATAGTG CAAGGTGAAC CCGTCAGTGA AATCGTGTGG 
TCGTGTAACG GCCAAGTGCT GGAGAACTCG GAGTGCTACC AGGCGCAGTA CAGGAACGGC 
GTGTGCAGAC TGACGATACC CAGGACGTAC CAATTCAATG CCGGATCGTA CTCGTGCACC 
GTATCCAATT ACCTGGGGTC CAGCAGCACG TCGGCCGACC TCCGAGTGTC AGGTGAGTCT 
CGCACGTTCA TTCAACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100169177 ( XM_029487061.1 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_029342921.1 Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X3, mRNA.

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CloneID	OAd09619
Clone ID Related Accession (Same CDS sequence)	XM_029487061.1
Accession Version	XM_029487061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	muscle M-line assembly protein unc-89 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGATGGAAC CGTTATTAGC 
CCCGTTTACC AAAAAGAACT GAACGCCGCC GAACACTCGG ATCCATCGCA CAGTTCACCT 
TTCTTCGTAA CCCCGTTAAA AGACATAGCT GTAGTTAGCG GCAAGACTGC CAGATTCGAG 
TGCATAGTGC AAGGTGAACC CGTCAGTGAA ATCGTGTGGT CGTGTAACGG CCAAGTGCTG 
GAGAACTCGG AGTGCTACCA GGCGCAGTAC AGGAACGGCG TGTGCAGACT GACGATACCC 
AGGACGTACC AATTCAATGC CGGATCGTAC TCGTGCACCG TATCCAATTA CCTGGGGTCC 
AGCAGCACGT CGGCCGACCT CCGAGTGTCA GGTGAGTCTC GCACGTTCAT TCAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029342921.1
CDS	605..10204
Translation	MESDDDYYTVSECLSSKVSSYGSCTPIASDQDEEFDTDTVVREVLQTRKLMLSPVRGCVETSEYIYKDTPILRTEKQPKCRDVLELKVLELCPELTLLGETLEEASSLQAEHERVLEKIQEKQSPVNELLKKADYLIATQNTQPQVYAAMADSLGLAWKDVNDVLQKRSYLLHLNVEYHRQAEVCMERIGTLEMMCHSYLPTDVESIKNHLEHIQEVRKSILEALMVALGNGTQLLDVLKEMQAKGTLDSRPGYNYHSISLAVSQVEHWLEKLHDKRHSLDIDCHERKTMLEKRLTISLLVTDLNLLENILSERRNSLERVKNCLGDSQYECSKFTEVNSNLITEAKDIRDQALRVARATEQLVLTKGYMEEDYKFKAYKLLELATEYLEELNRRETLLDAAMKFFESAEKVLRAWETILQHSSDEQLASKNTYVTETVKRTGASVIQEGNLILKDLPEAEGVKRVVDELERKKQLILSIVMKDRDKYMESSEAVNTFSENYNKIFSWLVSLAESFLHEHNSMGTNLTEAREFLISHKTLQRDLRTRGIDINALVNSTSLPPLTYIDAETRVDLEGKSIVLKDHWTYLDRILEWRIQLSELYVKFYTSCEHLNIEFSNLDSIIGDHVDVSDQRLDSAHKYWTNILQLYIQLKNTGEKFLHQSSKSEDAHLELDVARDRVRSLLDTFVDRKTRTTSVWETWQRASAENKLDKVLWSETMCESSKTVDWVSKLESQLYPVLKSEPSSVDRHISEVKQKLNNVLPEVQRAQDDIESRIKTAEELIGKGNIQGDNISIPPRLKELHSRLVGITTEYQNLLDMFLSFFNSLQEFHDIVKESERGRPTDIFNHQLNEVEELLKKHNQSRQTVLDSFKTVYAENIRLMDKIKQQEPQKSGEQDMYVVRYLMENERNLWDQSCETYCQRIEQQQQLSQFDNDLIQINNNLNDLSQQLSSTRGQYGANLASAKSASLAFHYFEKTILLLEQRIETFINAANSLMNFDHNKSEHIREELNKLKSRWDTFQAQVLESRNHIDLCVQYFTLINEAQDWFHEGSILLMNIARKSTEVKTSEEASALLKEIEIFLKPGDIRQDERLNQIHELSVQLFGEHAIAEVPQVVTENKELLDSFSLIAKELKSFDDKLKSREKYEINNEVNQIITDTYEEIITSTSMTNGVSQNEGNVPLSKKIKLEEIHKPKPTIILPLQDNTVDEGKQFTFECSIEGEPSKVTWFKDNISIDNNPDYQTSKIDNRYILRIEETFVEDSAKFTCRAENDSDFIETSAYLSVKECAPEHQISPSVFTKLLSDLLVKEGDACSLSCKAEGNPLPTVQWFKDDICIDNSPQYQITYNNGEALLKIEHTNSSTHSGKYTCVATNRLGSDSTASKLLIDALEKPEDGPTFVVPLSNVMARAGQKLKLECEVETNKPPILIWFHNGKVMKEIKDFKVSENRGKINLIIPEAFPKDAGTYSLTIKTEQGEATSSCQVSVKGILPNETSDSEIASDLEPIKPSIPLLLKEQTVLEGKSVHLKCIIVGQPEPEVIWYHDGRPVKESKDIQLLFQGDQCSLVIKEAFIEDAGEYKVVAINSAGEASSTCVLSVEPKAVPDIDELVVSPKFTKLLSDVLVREGDSITLECNAEGCPKPEFKWIRNTVEIKPDQRITLSSDEDGTATLHIHNALSVDKGQYTVRAVNKAGEAKCFSHVIVKAISTFDTAVNPPTDKVQFEEKLEKPSFTETFPSVVNAFTGESVKFECIVVGKPAPKVRWLFNEKPVSGKSFLVSTSGDRQVLTIPEADTGHGGIVECVAENEAGKCKCVSSLKIKESEAVPLTMAYNGGGDLKHTVSEVYESSSKFESKQSTFVSSSSSISGDNAPKFKLDSFSLQSEKQSRQFGDNAPIQSESLRTEEYHNTDGKQSQHVSDLSSTNVSEEHQTSFFERGLYNQSGLVKDASQKSLLERNISKQSRKEMAPRFVSAPQGKIAEQGKDVFLDAIIDSYPPSDITWSKNGIDLVPDGVKLKITSEVNKTRLDIKRLTVEDGGQYTCKAMNTAGGTSCTTDVIVKKNVFPPVMCRRLLPNTVAEGERVVLEIEVAGTPEPTVSWFKNNQPLSATSSLYRLRQQGSCSAVVIDKATTEHAGEYKVMAKNEGGEAVSCADLRVVQPLPDIGLNLLPTYAPNDNTDKVNVDYSKSIVPTNQSKQFNITSKTEEKSHFSKSVFVQESINSSKDVIMTVNRSEANDFDLNSNNANANYENTEKQISVQNENIENASITKKSALQFFKNVIEDNKVEKSSNSTIPPSAGIYKRESPIDNSSNFASDVHNKTYNIEENVSSSSFKQSNDTEVILEPGPPPTFDYMPRAQSVKKDQMTERLRRLSTNQKLLSPEQIPSGAVRIFPDVTSEIKQEVVEESCIKKEVIKSGLSEDTIAHTHLPTSYTPLPHSEPHVPLLRPQADIEVRPGSPRPSAEAISMEKLWTKGHTPHVQKTPVSYSPIHMKTYSSTESQSFVSETIEKNGELVKNESREEKQGSLKIDDGVKKVSESFSEISIRPTKHVEPPRNEIKRPASAHLFNEVKSKDAFFGNKVQTQHFSEESSQIKKSSMFDVKSNISEKVERNSSNVKSEHQLQNNQSFDRNMQNTYQFLPERSIGPIKHVEPPNNSKIISNFTRSHSVETAKEKQWLSQSPVAFRGPPQNKTYSSFEAHSSFSKTLEKDGVLVASEHNEEGGRVVDDGVQKAYDSFSERSKLPTKFVDLSKNTNKSNSIKTMQKMFEQTGSSSTVSNVGSNVRPYSRIKSRASDSDFESEAELSKYNVEQKVSESFNSFETKMYSSKTQLQEIKNTSIIMQSPIKESGYAADTDEPRGFQSESVVNNQTFNSKRNSGFQSTSIKKAEFLENERRQPHRAHPTQWANQYNQHSRTQVRSPAKFVRGEFRESDYESDYECRIQPIWRPYDSESEEPVYKPVRPGFKTPVKKPEGSRLLQPVQRSAVPPVVAFELKPGSPPEMGFAPSPDVHRSVSFVESERDESVQRTTHFLSSDGRSHGTLERNAEAKRLQRVDEMRKRFEQKSLCPAESSSVQQTVSSQLGQDGTVISPVYQKELNAAEHSDPSHSSPFFVTPLKDIAVVSGKTARFECIVQGEPVSEIVWSCNGQVLENSECYQAQYRNGVCRLTIPRTYQFNAGSYSCTVSNYLGSSSTSADLRVSGESRTFIQH

Target ORF information:

RefSeq Version	XM_029487061.1
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum muscle M-line assembly protein unc-89 (LOC100169177), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029487061.1

ORF Insert Sequence:

ATGGAGAGTG ATGATGATTA TTATACCGTT TCGGAGTGTC TAAGTTCAAA AGTTTCTTCA 
TATGGATCAT GTACGCCTAT CGCTTCTGAT CAAGATGAAG AATTTGATAC GGACACTGTC 
GTCCGAGAAG TGTTGCAGAC TAGAAAATTA ATGCTTTCGC CTGTTCGAGG ATGTGTGGAA 
ACTAGTGAAT ATATTTATAA AGATACGCCA ATACTGCGAA CTGAAAAACA GCCTAAATGT 
CGTGACGTTT TAGAATTAAA AGTTCTCGAA CTATGCCCGG AACTTACGTT GCTCGGGGAG 
ACGTTGGAAG AGGCCTCGAG CTTACAGGCC GAACACGAGA GAGTGTTGGA AAAAATACAG 
GAAAAGCAGA GTCCCGTCAA CGAGTTGTTG AAGAAAGCCG ATTATCTAAT AGCTACACAG 
AACACGCAGC CCCAGGTGTA TGCCGCTATG GCCGATTCGC TGGGATTAGC CTGGAAAGAC 
GTAAACGATG TACTTCAAAA GAGAAGTTAC TTGTTACACC TGAACGTTGA ATATCACCGA 
CAAGCTGAAG TATGCATGGA ACGAATCGGA ACTTTGGAAA TGATGTGCCA CAGTTACCTG 
CCGACCGACG TCGAATCGAT CAAAAACCAT CTGGAGCATA TCCAGGAAGT CCGCAAGTCT 
ATACTGGAAG CGCTAATGGT CGCTTTGGGC AATGGCACGC AGCTACTGGA CGTTTTGAAA 
GAGATGCAAG CCAAAGGAAC CCTGGATTCG AGGCCGGGAT ATAATTATCA TTCTATTTCA 
TTAGCCGTGT CTCAAGTAGA ACATTGGTTG GAGAAACTTC ACGATAAACG TCATTCGTTG 
GACATTGACT GTCACGAACG TAAGACAATG TTGGAAAAAC GACTGACAAT ATCTTTACTA 
GTCACCGATC TAAATTTACT TGAAAACATA CTATCTGAAA GAAGAAATTC TCTGGAAAGA 
GTTAAAAATT GTCTAGGCGA CTCCCAGTAT GAATGTTCTA AATTTACGGA AGTCAATTCG 
AACTTGATAA CCGAAGCAAA GGATATAAGA GACCAAGCGC TGAGGGTTGC AAGAGCCACC 
GAACAACTGG TATTAACTAA AGGCTACATG GAAGAGGATT ATAAATTTAA AGCGTATAAA 
CTATTAGAAT TAGCTACCGA ATACTTGGAA GAACTAAATA GAAGAGAAAC TCTTTTAGAT 
GCCGCTATGA AATTTTTCGA GTCGGCTGAA AAAGTTCTCC GAGCATGGGA AACCATACTT 
CAGCATTCAT CCGATGAGCA ATTGGCATCA AAGAACACGT ACGTTACAGA GACGGTAAAG 
CGAACCGGCG CCTCAGTAAT TCAAGAAGGA AATTTAATAC TGAAAGACTT ACCAGAGGCA 
GAGGGCGTTA AACGGGTTGT AGACGAGTTG GAAAGGAAAA AACAGCTAAT ACTGTCAATT 
GTTATGAAAG ACCGCGACAA ATATATGGAA TCTTCAGAAG CAGTCAACAC GTTTTCAGAG 
AATTATAATA AAATATTTTC CTGGCTTGTA AGTTTAGCTG AATCGTTTTT ACACGAACAT 
AATTCTATGG GTACAAATCT CACGGAAGCG CGAGAGTTTT TAATATCCCA TAAAACTTTA 
CAAAGAGATC TAAGGACTCG AGGGATCGAC ATTAACGCAT TGGTAAACTC GACTAGTCTG 
CCACCGTTAA CGTACATCGA TGCTGAAACA AGAGTCGATC TGGAAGGCAA ATCAATCGTG 
TTAAAAGACC ATTGGACTTA TTTGGATAGA ATATTGGAAT GGAGGATACA ATTGTCCGAG 
TTGTATGTCA AATTTTACAC GTCGTGTGAA CATTTGAACA TCGAATTTTC AAACTTGGAC 
TCGATCATTG GAGATCACGT CGACGTGTCT GATCAAAGAC TAGATTCGGC GCACAAGTAT 
TGGACAAACA TTTTACAATT GTATATCCAA CTTAAGAACA CAGGAGAAAA GTTTTTGCAT 
CAAAGTTCAA AATCGGAGGA CGCTCATCTG GAACTAGACG TAGCACGGGA CCGCGTGCGA 
TCGCTACTGG ACACGTTCGT GGACAGGAAA ACGAGAACTA CTTCCGTCTG GGAGACTTGG 
CAGCGGGCAT CGGCTGAAAA CAAACTGGAT AAAGTGCTCT GGTCGGAGAC GATGTGCGAG 
AGCAGCAAGA CAGTAGATTG GGTTTCCAAA TTGGAATCTC AATTATATCC AGTTTTGAAA 
TCTGAGCCGT CATCAGTTGA TCGACATATT TCAGAGGTTA AACAGAAGTT AAATAATGTT 
CTACCGGAAG TTCAAAGGGC TCAAGACGAT ATCGAGTCCA GAATAAAAAC AGCCGAAGAA 
CTTATTGGAA AAGGAAATAT TCAAGGTGAT AATATTTCTA TACCTCCAAG GTTAAAAGAA 
CTTCATAGCC GTCTTGTTGG AATCACTACA GAGTATCAAA ATCTTTTAGA TATGTTCTTA 
TCATTTTTTA ATTCTCTACA AGAATTTCAC GATATTGTGA AAGAATCTGA AAGAGGTAGG 
CCCACCGACA TTTTCAACCA TCAATTGAAC GAAGTAGAAG AGCTCCTTAA GAAACACAAC 
CAATCAAGAC AAACAGTTTT AGATAGTTTT AAAACTGTTT ATGCTGAAAA TATTCGTTTA 
ATGGATAAAA TTAAGCAACA GGAACCTCAA AAATCTGGAG AGCAAGATAT GTATGTAGTT 
CGTTATTTGA TGGAAAATGA AAGAAACCTT TGGGATCAAT CATGTGAAAC TTATTGTCAA 
AGAATCGAAC AACAGCAACA ATTAAGTCAG TTTGACAATG ATCTCATTCA GATCAACAAT 
AATTTAAATG ATCTTTCACA ACAACTGTCA TCTACGCGAG GGCAATATGG TGCAAATTTA 
GCATCTGCCA AGTCAGCTTC ACTTGCCTTT CATTATTTCG AAAAAACTAT ATTACTTTTA 
GAACAACGAA TCGAAACATT CATTAACGCA GCAAACTCAT TGATGAACTT TGATCATAAT 
AAGTCTGAAC ACATCCGAGA AGAATTAAAT AAACTGAAGT CAAGATGGGA TACGTTCCAA 
GCACAAGTGT TAGAAAGTCG TAACCATATT GATCTGTGTG TTCAATATTT TACGTTAATT 
AATGAAGCAC AAGATTGGTT CCACGAAGGA AGTATACTTC TTATGAACAT TGCTCGAAAA 
TCAACCGAAG TCAAAACAAG CGAAGAAGCT TCAGCATTAT TAAAAGAAAT TGAAATATTT 
TTAAAACCTG GTGATATAAG ACAAGATGAG AGATTAAATC AAATACACGA ACTTAGCGTT 
CAACTTTTTG GAGAACATGC AATAGCTGAA GTACCTCAAG TTGTTACAGA AAATAAAGAG 
CTACTTGATT CATTCTCATT GATTGCAAAA GAATTAAAGT CTTTTGACGA TAAGCTTAAA 
TCTAGAGAAA AATATGAAAT AAATAATGAA GTTAATCAAA TAATTACTGA TACGTACGAA 
GAAATAATAA CATCCACATC AATGACTAAT GGCGTGTCAC AAAATGAAGG TAATGTTCCA 
TTATCAAAGA AAATAAAACT CGAAGAAATC CATAAACCCA AACCTACGAT AATTCTACCA 
TTGCAAGATA ACACGGTTGA TGAAGGGAAA CAGTTTACAT TTGAATGCAG CATTGAAGGG 
GAACCTTCAA AAGTAACCTG GTTCAAGGAC AACATTTCCA TCGACAATAA TCCAGATTAT 
CAGACATCTA AAATTGATAA TCGATATATA TTGAGAATCG AGGAAACGTT TGTAGAAGAT 
TCAGCAAAAT TTACATGTCG GGCCGAAAAC GATTCTGATT TTATTGAAAC AAGTGCATAT 
CTTTCAGTTA AAGAATGTGC ACCTGAACAC CAAATTAGTC CATCTGTATT TACAAAACTA 
CTATCAGATT TGCTAGTAAA AGAAGGCGAT GCTTGTTCGT TATCTTGCAA AGCAGAAGGT 
AATCCACTCC CAACAGTGCA GTGGTTTAAA GATGACATTT GTATCGATAA TAGCCCTCAA 
TACCAAATTA CATATAATAA TGGCGAAGCA TTATTAAAAA TTGAACACAC CAATTCGTCT 
ACCCACAGTG GAAAATATAC TTGTGTTGCA ACTAACAGAC TTGGTTCAGA TTCAACTGCG 
TCAAAGTTAT TAATTGATGC TCTGGAAAAG CCTGAAGATG GGCCAACGTT TGTTGTACCT 
CTATCCAATG TAATGGCTAG AGCCGGTCAA AAATTGAAAT TGGAATGTGA AGTTGAAACA 
AATAAGCCAC CGATATTAAT TTGGTTCCAT AATGGGAAAG TCATGAAAGA AATAAAAGAT 
TTCAAGGTTT CTGAAAATCG TGGAAAAATT AATTTAATCA TTCCGGAAGC CTTTCCCAAA 
GACGCTGGCA CTTACAGTTT AACAATCAAA ACGGAACAGG GTGAAGCGAC CAGTAGTTGT 
CAAGTATCGG TGAAAGGTAT ACTCCCAAAT GAAACTTCCG ATTCGGAAAT TGCAAGTGAT 
TTGGAACCGA TCAAACCGTC AATACCGTTA CTTTTAAAAG AACAAACCGT TTTAGAAGGC 
AAAAGTGTTC ATTTGAAATG CATAATTGTT GGACAACCCG AACCAGAGGT AATATGGTAT 
CATGACGGAA GACCCGTTAA AGAATCCAAA GACATTCAAC TTTTATTCCA AGGTGATCAG 
TGTTCACTTG TAATCAAGGA GGCTTTTATC GAAGACGCGG GTGAATACAA AGTAGTGGCT 
ATTAACTCGG CAGGAGAAGC AAGCAGCACA TGCGTGTTAA GTGTCGAGCC TAAAGCAGTA 
CCAGATATAG ACGAACTAGT AGTTTCACCA AAGTTCACTA AGCTGCTGTC GGACGTATTA 
GTGAGGGAAG GTGATTCTAT TACTCTAGAA TGTAATGCCG AAGGTTGTCC GAAACCCGAA 
TTTAAATGGA TTAGGAACAC TGTGGAAATC AAGCCCGACC AAAGGATAAC ATTGAGTTCG 
GACGAAGATG GTACGGCGAC TCTTCACATA CATAACGCTT TGTCCGTGGA CAAAGGACAG 
TACACCGTGA GAGCCGTGAA TAAAGCCGGA GAAGCCAAAT GTTTTTCGCA CGTGATTGTA 
AAAGCCATTT CGACGTTTGA TACCGCGGTC AACCCACCGA CGGACAAAGT CCAATTCGAA 
GAAAAGTTGG AAAAACCATC ATTTACCGAG ACGTTTCCGT CAGTCGTGAA TGCATTCACC 
GGCGAAAGTG TGAAATTCGA ATGCATCGTT GTTGGCAAAC CCGCTCCAAA GGTCCGTTGG 
CTTTTCAACG AGAAGCCGGT GAGTGGAAAA TCGTTTTTGG TGTCTACCAG TGGCGATCGT 
CAGGTACTCA CCATCCCCGA AGCGGACACC GGGCACGGCG GAATAGTCGA GTGCGTGGCC 
GAGAACGAAG CAGGCAAATG CAAATGTGTG TCTTCGCTTA AAATAAAAGA AAGCGAAGCT 
GTTCCATTAA CCATGGCCTA CAACGGCGGC GGTGATTTAA AACATACGGT TTCCGAAGTT 
TATGAATCCA GTTCGAAATT CGAGTCGAAA CAATCGACGT TCGTGTCGTC ATCGTCGTCG 
ATCAGCGGTG ACAACGCTCC TAAATTTAAA TTGGACTCTT TCAGTTTACA GTCGGAAAAA 
CAAAGTAGGC AGTTTGGAGA CAACGCTCCA ATACAGTCTG AATCTTTACG AACGGAAGAG 
TACCACAACA CGGACGGAAA GCAAAGTCAA CATGTGAGTG ATCTGAGTTC CACCAACGTA 
TCCGAAGAAC ATCAAACTTC ATTTTTCGAA CGTGGACTGT ACAACCAAAG CGGGCTCGTT 
AAGGACGCCT CACAAAAATC TTTGCTCGAA AGAAACATTT CGAAACAATC AAGGAAAGAA 
ATGGCACCGC GTTTCGTGTC GGCTCCACAA GGAAAAATCG CTGAACAGGG AAAAGACGTA 
TTTTTGGATG CTATAATAGA CAGTTATCCG CCATCTGACA TCACGTGGAG CAAAAACGGT 
ATCGACTTGG TTCCCGACGG TGTGAAATTG AAAATAACCA GCGAAGTCAA CAAAACTCGG 
TTGGACATTA AACGTCTAAC TGTGGAAGAC GGTGGCCAAT ATACGTGCAA GGCTATGAAC 
ACCGCTGGGG GCACGAGTTG TACAACAGAC GTCATTGTTA AAAAAAACGT ATTTCCACCG 
GTCATGTGTC GAAGACTTTT ACCGAACACG GTCGCTGAAG GCGAAAGAGT AGTTTTAGAA 
ATTGAAGTGG CCGGTACGCC CGAACCAACA GTGAGTTGGT TTAAAAATAA TCAACCACTA 
TCGGCCACGT CGAGTTTATA CAGATTAAGA CAACAGGGGT CTTGTAGTGC CGTAGTTATC 
GATAAAGCTA CTACAGAACA TGCTGGAGAG TACAAAGTTA TGGCGAAAAA CGAAGGCGGG 
GAGGCTGTAA GTTGTGCCGA TTTGAGAGTG GTTCAGCCTT TACCCGATAT CGGATTAAAT 
TTGTTACCCA CTTATGCACC GAATGACAAC ACGGATAAAG TGAATGTGGA TTACTCCAAA 
AGTATTGTAC CGACAAATCA ATCGAAGCAA TTTAATATTA CTAGCAAAAC AGAAGAGAAA 
TCACATTTTT CTAAATCTGT CTTCGTTCAA GAATCGATAA ACAGCAGTAA GGATGTAATT 
ATGACTGTTA ACCGCTCTGA AGCAAATGAC TTTGATTTAA ATTCGAATAA TGCAAACGCT 
AATTATGAAA ATACTGAAAA ACAGATATCT GTTCAAAACG AAAATATCGA AAATGCATCG 
ATAACGAAAA AAAGTGCACT CCAGTTCTTT AAAAATGTCA TTGAAGATAA TAAAGTTGAA 
AAGTCTAGTA ATTCAACTAT TCCGCCATCA GCAGGAATTT ATAAAAGAGA AAGTCCCATA 
GATAATTCAA GTAATTTTGC ATCCGATGTT CATAATAAGA CGTATAATAT TGAAGAAAAC 
GTATCAAGTA GCTCATTTAA ACAGTCAAAC GACACGGAAG TCATTTTAGA ACCAGGTCCA 
CCGCCCACTT TTGATTATAT GCCAAGAGCG CAATCTGTAA AAAAAGATCA AATGACCGAA 
CGGTTGAGAA GACTATCAAC AAATCAAAAA TTATTATCAC CCGAACAAAT TCCGTCCGGC 
GCTGTTAGAA TATTTCCTGA CGTAACCTCT GAGATAAAAC AAGAAGTTGT TGAGGAAAGT 
TGTATAAAAA AAGAAGTTAT TAAATCTGGG TTATCCGAAG ACACAATAGC ACACACACAT 
TTGCCTACAT CCTATACGCC ACTGCCACAC AGTGAACCTC ATGTTCCACT TTTGAGACCC 
CAAGCCGACA TAGAAGTGAG ACCAGGATCT CCAAGACCTT CTGCAGAAGC TATTTCTATG 
GAAAAGCTAT GGACAAAAGG GCATACTCCA CATGTTCAAA AAACTCCAGT ATCGTATTCA 
CCGATTCATA TGAAAACATA TTCATCGACG GAAAGCCAAT CGTTTGTTTC AGAGACCATT 
GAAAAAAATG GTGAACTGGT AAAAAATGAA AGCAGAGAAG AGAAACAAGG TAGTCTAAAA 
ATAGACGATG GTGTTAAAAA AGTTTCAGAA TCGTTTTCAG AAATATCAAT TCGTCCGACG 
AAACACGTCG AACCACCACG AAATGAAATT AAACGCCCAG CATCAGCACA TTTGTTTAAT 
GAAGTAAAGT CTAAAGATGC GTTCTTCGGC AACAAAGTCC AAACACAACA TTTTTCTGAA 
GAATCGTCAC AAATTAAAAA AAGTTCAATG TTTGACGTGA AATCAAATAT TTCTGAAAAA 
GTAGAAAGAA ACAGTTCCAA TGTAAAATCT GAACATCAAT TGCAAAATAA TCAATCGTTT 
GATAGAAATA TGCAAAACAC ATATCAATTT TTACCAGAAC GTTCAATAGG TCCAATAAAA 
CACGTGGAAC CACCGAACAA CAGCAAGATA ATTTCTAATT TTACACGGTC ACACAGCGTA 
GAAACTGCCA AGGAAAAGCA ATGGTTGTCT CAATCACCGG TAGCTTTTAG AGGACCGCCT 
CAAAATAAAA CGTATTCATC GTTTGAAGCG CATTCAAGTT TTTCAAAAAC CTTAGAAAAG 
GACGGAGTGT TAGTAGCCTC CGAACATAAC GAAGAAGGCG GTCGAGTAGT GGACGACGGC 
GTTCAAAAAG CATATGATTC ATTTTCAGAA CGTTCAAAAT TACCGACAAA GTTTGTGGAC 
CTTTCAAAAA ATACTAATAA ATCAAATTCT ATAAAAACCA TGCAAAAAAT GTTCGAGCAG 
ACTGGTTCGT CGTCTACGGT CTCTAATGTT GGTTCAAACG TAAGACCATA CAGTAGAATT 
AAGTCCCGAG CAAGTGATAG CGATTTCGAA AGTGAAGCGG AATTGTCAAA ATACAACGTA 
GAGCAAAAAG TTTCAGAAAG TTTTAACAGT TTTGAAACGA AAATGTATTC TTCAAAAACT 
CAATTACAAG AAATTAAAAA CACTTCGATA ATCATGCAAA GTCCGATTAA GGAAAGTGGT 
TACGCGGCTG ACACGGACGA GCCAAGGGGA TTTCAGAGCG AATCGGTAGT TAATAATCAA 
ACGTTTAACT CCAAAAGGAA TTCGGGGTTT CAAAGCACTT CAATAAAGAA GGCTGAGTTT 
TTGGAAAACG AACGCCGTCA ACCGCACCGG GCACATCCGA CGCAGTGGGC AAACCAGTAC 
AATCAACACA GTCGGACTCA AGTCCGGAGC CCCGCGAAGT TCGTCAGGGG TGAATTCCGA 
GAGAGCGATT ACGAGAGCGA CTACGAGTGC AGGATACAAC CAATATGGCG CCCGTACGAT 
TCGGAATCGG AGGAACCGGT GTACAAGCCG GTCAGGCCTG GTTTCAAGAC TCCCGTAAAA 
AAGCCCGAGG GGTCGCGACT CTTGCAGCCA GTGCAGCGAT CGGCGGTGCC GCCGGTGGTG 
GCTTTCGAAC TGAAACCAGG GTCACCGCCC GAAATGGGTT TCGCGCCGTC GCCGGACGTG 
CATAGGTCGG TGAGCTTCGT CGAGTCCGAA CGCGACGAAA GCGTGCAGAG GACCACACAC 
TTCTTGTCGT CCGACGGTCG CAGTCACGGG ACGCTGGAGC GAAATGCCGA GGCGAAGCGG 
TTACAAAGGG TAGACGAGAT GAGGAAGAGG TTCGAACAAA AGTCACTGTG TCCGGCCGAA 
TCGAGTTCGG TGCAACAGAC GGTCTCGTCT CAACTCGGGC AAGATGGAAC CGTTATTAGC 
CCCGTTTACC AAAAAGAACT GAACGCCGCC GAACACTCGG ATCCATCGCA CAGTTCACCT 
TTCTTCGTAA CCCCGTTAAA AGACATAGCT GTAGTTAGCG GCAAGACTGC CAGATTCGAG 
TGCATAGTGC AAGGTGAACC CGTCAGTGAA ATCGTGTGGT CGTGTAACGG CCAAGTGCTG 
GAGAACTCGG AGTGCTACCA GGCGCAGTAC AGGAACGGCG TGTGCAGACT GACGATACCC 
AGGACGTACC AATTCAATGC CGGATCGTAC TCGTGCACCG TATCCAATTA CCTGGGGTCC 
AGCAGCACGT CGGCCGACCT CCGAGTGTCA GGTGAGTCTC GCACGTTCAT TCAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100169177 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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