Dpse\GA13474 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA13474
Entrez Gene ID	4803740
Full Name	GA13474 gene product from transcript GA13474-RA
Synonyms	GA13474,dpse_GLEANR_14750
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015183820.1	XP_015039306.1	uncharacterized protein, isoform B
XM_001360369.3	XP_001360406.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA13474 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA13474 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe03044	XM_001360369.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe22480	XM_015183820.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA13474 ( XM_001360369.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360406.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13474), mRNA.

Price & Availability↑

CloneID	ODe03044
Clone ID Related Accession (Same CDS sequence)	XM_001360369.3
Accession Version	XM_001360369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360369.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGAG GCATGCAACT TACCGCCATT GGGCTGTGCC TATTGGCCAC AGCTGCCACC 
GTTGTCCGGG CCCAGGAATC ATCGGACAAG TGGTCGCGGC AGCTCGAGGA TGCTCCCACC 
TCCGACACGG ATTCCCTGGA TTGGATACCA CTCGCCCAGC CGCTGGACGG AAGCGGCGGA 
AAGGATCGTT CCGGCAACGG TCGTGTGCTG ACCTACTCGC AGACGTTTCC ACCCAGCGCC 
CGTTTCGGGG ACCTGAACAA GCCAGGAGTA TCCCCCGGAT CGCAGTTTGA TTTTTCGTAC 
CAGTTTCCAA GGTACTCGAA TGGACAGGGA CAAGCCCTGC CCCTGCGACA GGGGCCACCG 
TCATCGCTGA AGACGAACTC CGGCCAGGAG CCCACATCGC AGTCGTTCTT CAAGTTTGAG 
ATGCCCTTCC ACAGTAACGA GGGGGGACAG GCCCAGGCGC CGCCCACTCT GCAGACGGGC 
TATCAGATCC AGCACACCTT TGGAGGCGGT CTTCAGGCCC CATCTTCGCT CTTCAGTCCT 
CCCTCACTGT TTGGCCAGCA GCAGGCGCAG CCCTTCGGCT CCGAGTTCCA TACCCTGCCG 
CAGAAGCCAC TGGCACAGAC CCTGAACAAG CCCCTCCGGC AGCAAAACTA CATCCAGGAC 
ATCCTGCCGC CAAAGTCCCA TGCGAAGCCG ACGCAGCCAT CGCTGCAGAG CGTGACCCCC 
CAGGTCTTTC CCCTCGATCC CGAGCCAGAG TCGATCTTTG GGTTGAACAA GCCGGCGCCC 
TCGTCCGCTC CGGCCTCGAG TGGAGGCCGG CAGCAGGAGG TCCAGCTGCT GTACGTGCCC 
TACGACACTC TGTACAACCA GCAGCGACAG CAGTCGGGCT CCAGGCCAGG ATCTTCCAAC 
TCCAACTTTG AGGTTAACAA GTTCAATGTG AATCCAGGAC TTCCTGCCGT GAATCCCTAC 
CAGATCAACC AGTTCTACAC CCAGGATCCC AACGTGGGCA GCGACTTCTT CAGCGCAGGA 
ACCACCTCTC GACCGAGCAC CACCACCAGC CAGCCGCATA GCATCTTCCA GAGCTTTGGC 
CAGTCCCAGC CGCAGCCACA ACCGCAGGCA CAGACGCAGT TCTCCACCGC CAAGCCCAAG 
CCGAAGGCTC ACCAGCCCCC GCTGGCGATG TTCCTGCTGC GCTCCAGCTC CCGGCCATCG 
CAGTCGGATG TGGTGGCTGC CCTGCGCAGT GCCCACAGCA TTTCCGTGAT CGATTCCCCA 
ACGAAACAGA CGCCGGAGAT CTTTGTGGGA CCCGCAGGAA TGCCCATTCC CGATGGCTAT 
GTGAAGTTTG ATCTTCCGTA TTTGTCGCAA CTGGCGCAGA CCCGCGACCT CAGCGATGTC 
TCCTTCTTCG TGGCTCCGCT CAGCTATCGT ACACCGAATG GATTCAACAA GATCCTCCTG 
CCCGAGCCTC ATGTGGGTTC CATTGTGGTC AACCGCGCCA AGGACTCTGT TTCGGTGCAG 
ACTCAGGCAC CGCCACAGCG TCAGCAGCGT CCCTATGCAG CCGTGAGCAG CACGCGGAAT 
CCCTTCGAGA CCACAACCCA GGCACAGGCA CAGTCCCCGT TGCAGTCGCA GTCACAGGGT 
GCATCGGGTG CACCTGTACG CGGGAACAAG TTCAGCTACT ATTATGTGCA GGACGGCATC 
CAGGAGGTGA GTTCTCCGAA GATTCCCGCC AAGCAGGAGC GCCACAAGAA GAAGCGACCC 
CAGAACGTTC AGGTGGAGCA TCAGCATCAT CAGCAGCAGC AGCAGCAGCC CATCTACCGT 
CCACCCACCA AGACGCAGAA CCCCTTCGAC ACGCTCAATC AGATCGGAGG CGATGACTTC 
TTCAAGTCGC TAACCAACAA GCCCACTACT ACCAGCTCCA CATCCACCAG CACAACATCG 
ACCACAACCT CGACGACATC CACCACGGCG GCGCCCACCC AGCCCCTCTT TACGTACAGC 
ACCCGGCCGC AGGTGGAATT CCCCGGCTCC AGTGGCCACC TGTATCCCGA GTACCTGCAG 
CCCGTCGTCC AGGAACAGCA GCCCCGTCTG ACCACGAGAC CGCCGACGGC CACCACAGTG 
GAAGAAGAGC ATCGCATGAA GCAGTACTTC CGGCAGCAAG ATGCCTTCCG CCAGCGTCCG 
CTCACTACGA TTCCGCCCGC CTTCGAACAG GTTCAGTACA CGCCCAGTGC ACCCGACTAC 
GAGGTGCCTA CGACGCAGAG GGTCAAGAGC AAGCATCGCG TGAACATCTA TACGCCTGCA 
GCAGTGCCGG TTACCACCTC GGATGTGGGC TACCAAGTGG ACCAAGAGCA GGCGCAGGCG 
CCGCTGAATC ACAGGCCCAG CTTCAACCAA GTGCAGAACG AGATTCTGGA TAACAGCAAC 
GTGGATTACG AGCCGAATCC CTACCATGTG CCGTCGGAGC TGCCGGCCTT GACGCCCGAC 
ATTCCCGGAT TGGTCAATAA TCTCCAGGAG AAAGACAAGC TCCAGCCAGG AACAACCATC 
ACGCCACAGA GCGAGCCCGA GGCGGAAACG CGACCCACCC GCCGTCCCCT GCTGCGCACC 
CGCAAGCCAG CCGTTTCCAA GGTGGTGACC ACCTCCTCCG TGTCGTACTC CGAGTCCGAG 
TCAAGCGGAA AGGTGCCGCA TCGCATCCGT CGACCATACG GTAACCGAGG CACTGCAGCC 
GGAACCGCCT CCACCACGGG CGGCGGCGGA GACCAGGGAG ACGACTCTGC ACCGATCCCC 
ACCCGGCGAC CAACCAGCGC CCGGAATCCC CTCATTCGGA ATCCAAATCG TATCCGCTAC 
AGGCCCACCA CCGAGGAGCG CCAGTCGCTG AAGGTGAAGA CCAGGAAGGG ATCAAAGAAC 
GGCCAACGCG AAGACCAGGA TCTCGACTAC CAACGTGATG TGCTCAAGCA GAACTATCCG 
GTGTTCAAGG GCTCCTCCTC CGCCCCCTCG CGCCGACCCA CAAGCCCCAC CGCCATTCCC 
AGTTCGTACG ACTTCCAGGC CACCACCGAA GGCCCGGCTT CGGAGTCACA GCAGGTGTAC 
ACGGTGACGC CCAGCAACAG CATCGACAGC GCCAGCGTTC AGGGTTCGGG ATTCCCGTCC 
AGCCTGCTGG AGCCCATGCA GATCGCCCAG CATTACCAGG AGTTCTCCAA GGACAACTAC 
GGGCCCGGCT ACTTCCCCAA TCAGGAGGAT CTCACACCCA CCGAGGCCAT TGTTCGCAAT 
GAGGTGAGTC CCATTATCAC CACGCTGGCT ACCACAACGC CCTCGACAAC TACGACCAGC 
ACCACGACAA CCACAGAGGC AGCGCCCACG ACCACAACAC GTCGCTCGCC GTTCGTGCGC 
CGAAACTATC CCAGGGTGAG GACTACCACC ACAACAGAGC CGCCAGTGAC CAGCACCACG 
CCCTCGGAGG AGCGTCCTTC CATTCGCACT CGGCTGCCGC CTCGTCGTGT GGTCAAGGTG 
CGACAACGTC AGCGTCGTCC AGGACACCCT GCCTCCTCCA CTGCGGCCCC CGAAGAGGAA 
GTCCAGGAAG CAGCAACCCA GAGGACAAAT CTCCGCAAGC TGAGCCGCTA CAATCAAGAG 
CCCAAAGAAA AAGAGACAAC CATCACCCAG CGGCGTCGCT ATAAGCAGCC ATTCCAGCTG 
GAGGGCCAAG AGTCCCAGTG GTCGCCATCC TCGGAGACTT CCAACAGCAA CAACAATGCC 
AATGCCAACA CCAACAGTTT CAAGCCACTG AATCCCAAGT ACAAGACAGA GTCCCACAAC 
TTCGAGAACG AGCCAGAGAT CGTGACTGTG GGACCCACAG CGCAGCCCGA GACGTACGAG 
TTCAACGTAG CTGCGGATCT GGCTGGATCA GCCGTGCAAC GCACCACCAC CCTAAAGGCG 
CCAAACCTGA AACCAGAGAA ATCCTTTGCT GAACTGCTCG AGGAAGTGAT GGGCAAGGTT 
CCCGAGGAAC TGGCCACAGA GTCACCCAAC ACCAGCAGCA CCATTGGCAG GCTGAATAGG 
CGTGGCAAGT GGAAGAAGAG CCGCGTGACT AGTGGATCGG AAAATGGCGA AAACTTCGAG 
ACGGCCGAGT CCCAGAATCT GGGACCTCAG CTCTACAATG CCCTGCAGGC GGCCAGCGAG 
AAGGAGGAGC AGCAGCCGAA GACCACCACC CCGCTGACAC CTGCAGCCAC CGAGGCTGAG 
ACAGCAGAAA CGATAGTCCC CACCACGGCT GCCTCGCCCA CGACACCAGA GGAGTATGAG 
GTGACCACCG CCAGAGCCTT GGAGGAGGCA ACACTGGCAT CGACGCTGCC AATGGAGACG 
CTGGAGAGTG CCACGCGTCG CATGGATACC GCGGCGGATG TGGATGTGGA TGGGGATGGC 
GACCTGGAGA CGCAGCCCAG CATCTTCTCA GAGGTGAAGA AGCAGCTGCA TGATCTGTTT 
GCCATCGAAG AGAGCGAGGA CGAGACGGTG AACGCTGCCC TGGCTGCCGT GGGTAAGCGT 
CGCCAGGAGT ACACGAGCAT CAAGCGCAGC ACTACTCCAG CCACGTCCAC GGACAAAACC 
ACTCTCTCAT CAGCGGACGA TGAGCTGACA GCCACCACCA CCACAGCAAC AGAAGGTGCT 
TTGGAGGGTA GCAAAGATAA CTTCCACAAG GACCTCATGG AGCATGTGGT TTATGCCACA 
TCCACATCGA CCAAAGTCAC CTCCGAGACA GAGATCTGCT ATCGCGGACG CTGCATAAGG 
TCCGAGGATC TGCCCGCCAA CCACAAACTG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360406.2
CDS	407..5182
Translation	MKRGMQLTAIGLCLLATAATVVRAQESSDKWSRQLEDAPTSDTDSLDWIPLAQPLDGSGGKDRSGNGRVLTYSQTFPPSARFGDLNKPGVSPGSQFDFSYQFPRYSNGQGQALPLRQGPPSSLKTNSGQEPTSQSFFKFEMPFHSNEGGQAQAPPTLQTGYQIQHTFGGGLQAPSSLFSPPSLFGQQQAQPFGSEFHTLPQKPLAQTLNKPLRQQNYIQDILPPKSHAKPTQPSLQSVTPQVFPLDPEPESIFGLNKPAPSSAPASSGGRQQEVQLLYVPYDTLYNQQRQQSGSRPGSSNSNFEVNKFNVNPGLPAVNPYQINQFYTQDPNVGSDFFSAGTTSRPSTTTSQPHSIFQSFGQSQPQPQPQAQTQFSTAKPKPKAHQPPLAMFLLRSSSRPSQSDVVAALRSAHSISVIDSPTKQTPEIFVGPAGMPIPDGYVKFDLPYLSQLAQTRDLSDVSFFVAPLSYRTPNGFNKILLPEPHVGSIVVNRAKDSVSVQTQAPPQRQQRPYAAVSSTRNPFETTTQAQAQSPLQSQSQGASGAPVRGNKFSYYYVQDGIQEVSSPKIPAKQERHKKKRPQNVQVEHQHHQQQQQQPIYRPPTKTQNPFDTLNQIGGDDFFKSLTNKPTTTSSTSTSTTSTTTSTTSTTAAPTQPLFTYSTRPQVEFPGSSGHLYPEYLQPVVQEQQPRLTTRPPTATTVEEEHRMKQYFRQQDAFRQRPLTTIPPAFEQVQYTPSAPDYEVPTTQRVKSKHRVNIYTPAAVPVTTSDVGYQVDQEQAQAPLNHRPSFNQVQNEILDNSNVDYEPNPYHVPSELPALTPDIPGLVNNLQEKDKLQPGTTITPQSEPEAETRPTRRPLLRTRKPAVSKVVTTSSVSYSESESSGKVPHRIRRPYGNRGTAAGTASTTGGGGDQGDDSAPIPTRRPTSARNPLIRNPNRIRYRPTTEERQSLKVKTRKGSKNGQREDQDLDYQRDVLKQNYPVFKGSSSAPSRRPTSPTAIPSSYDFQATTEGPASESQQVYTVTPSNSIDSASVQGSGFPSSLLEPMQIAQHYQEFSKDNYGPGYFPNQEDLTPTEAIVRNEVSPIITTLATTTPSTTTTSTTTTTEAAPTTTTRRSPFVRRNYPRVRTTTTTEPPVTSTTPSEERPSIRTRLPPRRVVKVRQRQRRPGHPASSTAAPEEEVQEAATQRTNLRKLSRYNQEPKEKETTITQRRRYKQPFQLEGQESQWSPSSETSNSNNNANANTNSFKPLNPKYKTESHNFENEPEIVTVGPTAQPETYEFNVAADLAGSAVQRTTTLKAPNLKPEKSFAELLEEVMGKVPEELATESPNTSSTIGRLNRRGKWKKSRVTSGSENGENFETAESQNLGPQLYNALQAASEKEEQQPKTTTPLTPAATEAETAETIVPTTAASPTTPEEYEVTTARALEEATLASTLPMETLESATRRMDTAADVDVDGDGDLETQPSIFSEVKKQLHDLFAIEESEDETVNAALAAVGKRRQEYTSIKRSTTPATSTDKTTLSSADDELTATTTTATEGALEGSKDNFHKDLMEHVVYATSTSTKVTSETEICYRGRCIRSEDLPANHKLH

Target ORF information:

RefSeq Version	XM_001360369.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360369.3

ORF Insert Sequence:

ATGAAGCGAG GCATGCAACT TACCGCCATT GGGCTGTGCC TATTGGCCAC AGCTGCCACC 
GTTGTCCGGG CCCAGGAATC ATCGGACAAG TGGTCGCGGC AGCTCGAGGA TGCTCCCACC 
TCCGACACGG ATTCCCTGGA TTGGATACCA CTCGCCCAGC CGCTGGACGG AAGCGGCGGA 
AAGGATCGTT CCGGCAACGG TCGTGTGCTG ACCTACTCGC AGACGTTTCC ACCCAGCGCC 
CGTTTCGGGG ACCTGAACAA GCCAGGAGTA TCCCCCGGAT CGCAGTTTGA TTTTTCGTAC 
CAGTTTCCAA GGTACTCGAA TGGACAGGGA CAAGCCCTGC CCCTGCGACA GGGGCCACCG 
TCATCGCTGA AGACGAACTC CGGCCAGGAG CCCACATCGC AGTCGTTCTT CAAGTTTGAG 
ATGCCCTTCC ACAGTAACGA GGGGGGACAG GCCCAGGCGC CGCCCACTCT GCAGACGGGC 
TATCAGATCC AGCACACCTT TGGAGGCGGT CTTCAGGCCC CATCTTCGCT CTTCAGTCCT 
CCCTCACTGT TTGGCCAGCA GCAGGCGCAG CCCTTCGGCT CCGAGTTCCA TACCCTGCCG 
CAGAAGCCAC TGGCACAGAC CCTGAACAAG CCCCTCCGGC AGCAAAACTA CATCCAGGAC 
ATCCTGCCGC CAAAGTCCCA TGCGAAGCCG ACGCAGCCAT CGCTGCAGAG CGTGACCCCC 
CAGGTCTTTC CCCTCGATCC CGAGCCAGAG TCGATCTTTG GGTTGAACAA GCCGGCGCCC 
TCGTCCGCTC CGGCCTCGAG TGGAGGCCGG CAGCAGGAGG TCCAGCTGCT GTACGTGCCC 
TACGACACTC TGTACAACCA GCAGCGACAG CAGTCGGGCT CCAGGCCAGG ATCTTCCAAC 
TCCAACTTTG AGGTTAACAA GTTCAATGTG AATCCAGGAC TTCCTGCCGT GAATCCCTAC 
CAGATCAACC AGTTCTACAC CCAGGATCCC AACGTGGGCA GCGACTTCTT CAGCGCAGGA 
ACCACCTCTC GACCGAGCAC CACCACCAGC CAGCCGCATA GCATCTTCCA GAGCTTTGGC 
CAGTCCCAGC CGCAGCCACA ACCGCAGGCA CAGACGCAGT TCTCCACCGC CAAGCCCAAG 
CCGAAGGCTC ACCAGCCCCC GCTGGCGATG TTCCTGCTGC GCTCCAGCTC CCGGCCATCG 
CAGTCGGATG TGGTGGCTGC CCTGCGCAGT GCCCACAGCA TTTCCGTGAT CGATTCCCCA 
ACGAAACAGA CGCCGGAGAT CTTTGTGGGA CCCGCAGGAA TGCCCATTCC CGATGGCTAT 
GTGAAGTTTG ATCTTCCGTA TTTGTCGCAA CTGGCGCAGA CCCGCGACCT CAGCGATGTC 
TCCTTCTTCG TGGCTCCGCT CAGCTATCGT ACACCGAATG GATTCAACAA GATCCTCCTG 
CCCGAGCCTC ATGTGGGTTC CATTGTGGTC AACCGCGCCA AGGACTCTGT TTCGGTGCAG 
ACTCAGGCAC CGCCACAGCG TCAGCAGCGT CCCTATGCAG CCGTGAGCAG CACGCGGAAT 
CCCTTCGAGA CCACAACCCA GGCACAGGCA CAGTCCCCGT TGCAGTCGCA GTCACAGGGT 
GCATCGGGTG CACCTGTACG CGGGAACAAG TTCAGCTACT ATTATGTGCA GGACGGCATC 
CAGGAGGTGA GTTCTCCGAA GATTCCCGCC AAGCAGGAGC GCCACAAGAA GAAGCGACCC 
CAGAACGTTC AGGTGGAGCA TCAGCATCAT CAGCAGCAGC AGCAGCAGCC CATCTACCGT 
CCACCCACCA AGACGCAGAA CCCCTTCGAC ACGCTCAATC AGATCGGAGG CGATGACTTC 
TTCAAGTCGC TAACCAACAA GCCCACTACT ACCAGCTCCA CATCCACCAG CACAACATCG 
ACCACAACCT CGACGACATC CACCACGGCG GCGCCCACCC AGCCCCTCTT TACGTACAGC 
ACCCGGCCGC AGGTGGAATT CCCCGGCTCC AGTGGCCACC TGTATCCCGA GTACCTGCAG 
CCCGTCGTCC AGGAACAGCA GCCCCGTCTG ACCACGAGAC CGCCGACGGC CACCACAGTG 
GAAGAAGAGC ATCGCATGAA GCAGTACTTC CGGCAGCAAG ATGCCTTCCG CCAGCGTCCG 
CTCACTACGA TTCCGCCCGC CTTCGAACAG GTTCAGTACA CGCCCAGTGC ACCCGACTAC 
GAGGTGCCTA CGACGCAGAG GGTCAAGAGC AAGCATCGCG TGAACATCTA TACGCCTGCA 
GCAGTGCCGG TTACCACCTC GGATGTGGGC TACCAAGTGG ACCAAGAGCA GGCGCAGGCG 
CCGCTGAATC ACAGGCCCAG CTTCAACCAA GTGCAGAACG AGATTCTGGA TAACAGCAAC 
GTGGATTACG AGCCGAATCC CTACCATGTG CCGTCGGAGC TGCCGGCCTT GACGCCCGAC 
ATTCCCGGAT TGGTCAATAA TCTCCAGGAG AAAGACAAGC TCCAGCCAGG AACAACCATC 
ACGCCACAGA GCGAGCCCGA GGCGGAAACG CGACCCACCC GCCGTCCCCT GCTGCGCACC 
CGCAAGCCAG CCGTTTCCAA GGTGGTGACC ACCTCCTCCG TGTCGTACTC CGAGTCCGAG 
TCAAGCGGAA AGGTGCCGCA TCGCATCCGT CGACCATACG GTAACCGAGG CACTGCAGCC 
GGAACCGCCT CCACCACGGG CGGCGGCGGA GACCAGGGAG ACGACTCTGC ACCGATCCCC 
ACCCGGCGAC CAACCAGCGC CCGGAATCCC CTCATTCGGA ATCCAAATCG TATCCGCTAC 
AGGCCCACCA CCGAGGAGCG CCAGTCGCTG AAGGTGAAGA CCAGGAAGGG ATCAAAGAAC 
GGCCAACGCG AAGACCAGGA TCTCGACTAC CAACGTGATG TGCTCAAGCA GAACTATCCG 
GTGTTCAAGG GCTCCTCCTC CGCCCCCTCG CGCCGACCCA CAAGCCCCAC CGCCATTCCC 
AGTTCGTACG ACTTCCAGGC CACCACCGAA GGCCCGGCTT CGGAGTCACA GCAGGTGTAC 
ACGGTGACGC CCAGCAACAG CATCGACAGC GCCAGCGTTC AGGGTTCGGG ATTCCCGTCC 
AGCCTGCTGG AGCCCATGCA GATCGCCCAG CATTACCAGG AGTTCTCCAA GGACAACTAC 
GGGCCCGGCT ACTTCCCCAA TCAGGAGGAT CTCACACCCA CCGAGGCCAT TGTTCGCAAT 
GAGGTGAGTC CCATTATCAC CACGCTGGCT ACCACAACGC CCTCGACAAC TACGACCAGC 
ACCACGACAA CCACAGAGGC AGCGCCCACG ACCACAACAC GTCGCTCGCC GTTCGTGCGC 
CGAAACTATC CCAGGGTGAG GACTACCACC ACAACAGAGC CGCCAGTGAC CAGCACCACG 
CCCTCGGAGG AGCGTCCTTC CATTCGCACT CGGCTGCCGC CTCGTCGTGT GGTCAAGGTG 
CGACAACGTC AGCGTCGTCC AGGACACCCT GCCTCCTCCA CTGCGGCCCC CGAAGAGGAA 
GTCCAGGAAG CAGCAACCCA GAGGACAAAT CTCCGCAAGC TGAGCCGCTA CAATCAAGAG 
CCCAAAGAAA AAGAGACAAC CATCACCCAG CGGCGTCGCT ATAAGCAGCC ATTCCAGCTG 
GAGGGCCAAG AGTCCCAGTG GTCGCCATCC TCGGAGACTT CCAACAGCAA CAACAATGCC 
AATGCCAACA CCAACAGTTT CAAGCCACTG AATCCCAAGT ACAAGACAGA GTCCCACAAC 
TTCGAGAACG AGCCAGAGAT CGTGACTGTG GGACCCACAG CGCAGCCCGA GACGTACGAG 
TTCAACGTAG CTGCGGATCT GGCTGGATCA GCCGTGCAAC GCACCACCAC CCTAAAGGCG 
CCAAACCTGA AACCAGAGAA ATCCTTTGCT GAACTGCTCG AGGAAGTGAT GGGCAAGGTT 
CCCGAGGAAC TGGCCACAGA GTCACCCAAC ACCAGCAGCA CCATTGGCAG GCTGAATAGG 
CGTGGCAAGT GGAAGAAGAG CCGCGTGACT AGTGGATCGG AAAATGGCGA AAACTTCGAG 
ACGGCCGAGT CCCAGAATCT GGGACCTCAG CTCTACAATG CCCTGCAGGC GGCCAGCGAG 
AAGGAGGAGC AGCAGCCGAA GACCACCACC CCGCTGACAC CTGCAGCCAC CGAGGCTGAG 
ACAGCAGAAA CGATAGTCCC CACCACGGCT GCCTCGCCCA CGACACCAGA GGAGTATGAG 
GTGACCACCG CCAGAGCCTT GGAGGAGGCA ACACTGGCAT CGACGCTGCC AATGGAGACG 
CTGGAGAGTG CCACGCGTCG CATGGATACC GCGGCGGATG TGGATGTGGA TGGGGATGGC 
GACCTGGAGA CGCAGCCCAG CATCTTCTCA GAGGTGAAGA AGCAGCTGCA TGATCTGTTT 
GCCATCGAAG AGAGCGAGGA CGAGACGGTG AACGCTGCCC TGGCTGCCGT GGGTAAGCGT 
CGCCAGGAGT ACACGAGCAT CAAGCGCAGC ACTACTCCAG CCACGTCCAC GGACAAAACC 
ACTCTCTCAT CAGCGGACGA TGAGCTGACA GCCACCACCA CCACAGCAAC AGAAGGTGCT 
TTGGAGGGTA GCAAAGATAA CTTCCACAAG GACCTCATGG AGCATGTGGT TTATGCCACA 
TCCACATCGA CCAAAGTCAC CTCCGAGACA GAGATCTGCT ATCGCGGACG CTGCATAAGG 
TCCGAGGATC TGCCCGCCAA CCACAAACTG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13474 ( XM_015183820.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039306.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13474), mRNA.

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CloneID	ODe22480
Clone ID Related Accession (Same CDS sequence)	XM_015183820.1
Accession Version	XM_015183820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGAG GCATGCAACT TACCGCCATT GGGCTGTGCC TATTGGCCAC AGCTGCCACC 
GTTGTCCGGG CCCAGGAATC ATCGGACAAG TGGTCGCGGC AGCTCGAGGA TGCTCCCACC 
TCCGACACGG ATTCCCTGGA TTGGATACCA CTCGCCCAGC CGCTGGACGG AAGCGGCGGA 
AAGGATCGTT CCGGCAACGG TCGTGTGCTG ACCTACTCGC AGACGTTTCC ACCCAGCGCC 
CGTTTCGGGG ACCTGAACAA GCCAGGAGTA TCCCCCGGAT CGCAGTTTGA TTTTTCGTAC 
CAGTTTCCAA GGTACTCGAA TGGACAGGGA CAAGCCCTGC CCCTGCGACA GGGGCCACCG 
TCATCGCTGA AGACGAACTC CGGCCAGGAG CCCACATCGC AGTCGTTCTT CAAGTTTGAG 
ATGCCCTTCC ACAGTAACGA GGGGGGACAG GCCCAGGCGC CGCCCACTCT GCAGACGGGC 
TATCAGATCC AGCACACCTT TGGAGGCGGT CTTCAGGCCC CATCTTCGCT CTTCAGTCCT 
CCCTCACTGT TTGGCCAGCA GCAGGCGCAG CCCTTCGGCT CCGAGTTCCA TACCCTGCCG 
CAGAAGCCAC TGGCACAGAC CCTGAACAAG CCCCTCCGGC AGCAAAACTA CATCCAGGAC 
ATCCTGCCGC CAAAGTCCCA TGCGAAGCCG ACGCAGCCAT CGCTGCAGAG CGTGACCCCC 
CAGGTCTTTC CCCTCGATCC CGAGCCAGAG TCGATCTTTG GGTTGAACAA GCCGGCGCCC 
TCGTCCGCTC CGGCCTCGAG TGGAGGCCGG CAGCAGGAGG TCCAGCTGCT GTACGTGCCC 
TACGACACTC TGTACAACCA GCAGCGACAG CAGTCGGGCT CCAGGCCAGG ATCTTCCAAC 
TCCAACTTTG AGGTTAACAA GTTCAATGTG AATCCAGGAC TTCCTGCCGT GAATCCCTAC 
CAGATCAACC AGTTCTACAC CCAGGATCCC AACGTGGGCA GCGACTTCTT CAGCGCAGGA 
ACCACCTCTC GACCGAGCAC CACCACCAGC CAGCCGCATA GCATCTTCCA GAGCTTTGGC 
CAGTCCCAGC CGCAGCCACA ACCGCAGGCA CAGACGCAGT TCTCCACCGC CAAGCCCAAG 
CCGAAGGCTC ACCAGCCCCC GCTGGCGATG TTCCTGCTGC GCTCCAGCTC CCGGCCATCG 
CAGTCGGATG TGGTGGCTGC CCTGCGCAGT GCCCACAGCA TTTCCGTGAT CGATTCCCCA 
ACGAAACAGA CGCCGGAGAT CTTTGTGGGA CCCGCAGGAA TGCCCATTCC CGATGGCTAT 
GTGAAGTTTG ATCTTCCGTA TTTGTCGCAA CTGGCGCAGA CCCGCGACCT CAGCGATGTC 
TCCTTCTTCG TGGCTCCGCT CAGCTATCGT ACACCGAATG GATTCAACAA GATCCTCCTG 
CCCGAGCCTC ATGTGGGTTC CATTGTGGTC AACCGCGCCA AGGACTCTGT TTCGGTGCAG 
ACTCAGGCAC CGCCACAGCG TCAGCAGCGT CCCTATGCAG CCGTGAGCAG CACGCGGAAT 
CCCTTCGAGA CCACAACCCA GGCACAGGCA CAGTCCCCGT TGCAGTCGCA GTCACAGGGT 
GCATCGGGTG CACCTGTACG CGGGAACAAG TTCAGCTACT ATTATGTGCA GGACGGCATC 
CAGGAGGTGA GTTCTCCGAA GATTCCCGCC AAGCAGGAGC GCCACAAGAA GAAGCGACCC 
CAGAACGTTC AGGTGGAGCA TCAGCATCAT CAGCAGCAGC AGCAGCAGCC CATCTACCGT 
CCACCCACCA AGACGCAGAA CCCCTTCGAC ACGCTCAATC AGATCGGAGG CGATGACTTC 
TTCAAGTCGC TAACCAACAA GCCCACTACT ACCAGCTCCA CATCCACCAG CACAACATCG 
ACCACAACCT CGACGACATC CACCACGGCG GCGCCCACCC AGCCCCTCTT TACGTACAGC 
ACCCGGCCGC AGGTGGAATT CCCCGGCTCC AGTGGCCACC TGTATCCCGA GTACCTGCAG 
CCCGTCGTCC AGGAACAGCA GCCCCGTCTG ACCACGAGAC CGCCGACGGC CACCACAGTG 
GAAGAAGAGC ATCGCATGAA GCAGTACTTC CGGCAGCAAG ATGCCTTCCG CCAGCGTCCG 
CTCACTACGA TTCCGCCCGC CTTCGAACAG GTTCAGTACA CGCCCAGTGC ACCCGACTAC 
GAGGTGCCTA CGACGCAGAG GGTCAAGAGC AAGCATCGCG TGAACATCTA TACGCCTGCA 
GCAGTGCCGG TTACCACCTC GGATGTGGGC TACCAAGTGG ACCAAGAGCA GGCGCAGGCG 
CCGCTGAATC ACAGGCCCAG CTTCAACCAA GTGCAGAACG AGATTCTGGA TAACAGCAAC 
GTGGATTACG AGCCGAATCC CTACCATGTG CCGTCGGAGC TGCCGGCCTT GACGCCCGAC 
ATTCCCGGAT TGGTCAATAA TCTCCAGGAG AAAGACAAGC TCCAGCCAGG AACAACCATC 
ACGCCACAGA GCGAGCCCGA GGCGGAAACG CGACCCACCC GCCGTCCCCT GCTGCGCACC 
CGCAAGCCAG CCGTTTCCAA GGTGGTGACC ACCTCCTCCG TGTCGTACTC CGAGTCCGAG 
TCAAGCGGAA AGGTGCCGCA TCGCATCCGT CGACCATACG GTAACCGAGG CACTGCAGCC 
GGAACCGCCT CCACCACGGG CGGCGGCGGA GACCAGGGAG ACGACTCTGC ACCGATCCCC 
ACCCGGCGAC CAACCAGCGC CCGGAATCCC CTCATTCGGA ATCCAAATCG TATCCGCTAC 
AGGCCCACCA CCGAGGAGCG CCAGTCGCTG AAGGTGAAGA CCAGGAAGGG ATCAAAGAAC 
GGCCAACGCG AAGACCAGGA TCTCGACTAC CAACGTGATG TGCTCAAGCA GAACTATCCG 
GTGTTCAAGG GCTCCTCCTC CGCCCCCTCG CGCCGACCCA CAAGCCCCAC CGCCATTCCC 
AGTTCGTACG ACTTCCAGGC CACCACCGAA GGCCCGGCTT CGGAGTCACA GCAGGTGTAC 
ACGGTGACGC CCAGCAACAG CATCGACAGC GCCAGCGTTC AGGGTTCGGG ATTCCCGTCC 
AGCCTGCTGG AGCCCATGCA GATCGCCCAG CATTACCAGG AGTTCTCCAA GGACAACTAC 
GGGCCCGGCT ACTTCCCCAA TCAGGAGGAT CTCACACCCA CCGAGGCCAT TGTTCGCAAT 
GAGGTGAGTC CCATTATCAC CACGCTGGCT ACCACAACGC CCTCGACAAC TACGACCAGC 
ACCACGACAA CCACAGAGGC AGCGCCCACG ACCACAACAC GTCGCTCGCC GTTCGTGCGC 
CGAAACTATC CCAGGGTGAG GACTACCACC ACAACAGAGC CGCCAGTGAC CAGCACCACG 
CCCTCGGAGG AGCGTCCTTC CATTCGCACT CGGCTGCCGC CTCGTCGTGT GGTCAAGGTG 
CGACAACGTC AGCGTCGTCC AGGACACCCT GCCTCCTCCA CTGCGGCCCC CGAAGAGGAA 
GTCCAGGAAG CAGCAACCCA GAGGACAAAT CTCCGCAAGC TGAGCCGCTA CAATCAAGAG 
CCCAAAGAAA AAGAGCAGAC AACCATCACC CAGCGGCGTC GCTATAAGCA GCCATTCCAG 
CTGGAGGGCC AAGAGTCCCA GTGGTCGCCA TCCTCGGAGA CTTCCAACAG CAACAACAAT 
GCCAATGCCA ACACCAACAG TTTCAAGCCA CTGAATCCCA AGTACAAGAC AGAGTCCCAC 
AACTTCGAGA ACGAGCCAGA GATCGTGACT GTGGGACCCA CAGCGCAGCC CGAGACGTAC 
GAGTTCAACG TAGCTGCGGA TCTGGCTGGA TCAGCCGTGC AACGCACCAC CACCCTAAAG 
GCGCCAAACC TGAAACCAGA GAAATCCTTT GCTGAACTGC TCGAGGAAGT GATGGGCAAG 
GTTCCCGAGG AACTGGCCAC AGAGTCACCC AACACCAGCA GCACCATTGG CAGGCTGAAT 
AGGCGTGGCA AGTGGAAGAA GAGCCGCGTG ACTAGTGGAT CGGAAAATGG CGAAAACTTC 
GAGACGGCCG AGTCCCAGAA TCTGGGACCT CAGCTCTACA ATGCCCTGCA GGCGGCCAGC 
GAGAAGGAGG AGCAGCAGCC GAAGACCACC ACCCCGCTGA CACCTGCAGC CACCGAGGCT 
GAGACAGCAG AAACGATAGT CCCCACCACG GCTGCCTCGC CCACGACACC AGAGGAGTAT 
GAGGTGACCA CCGCCAGAGC CTTGGAGGAG GCAACACTGG CATCGACGCT GCCAATGGAG 
ACGCTGGAGA GTGCCACGCG TCGCATGGAT ACCGCGGCGG ATGTGGATGT GGATGGGGAT 
GGCGACCTGG AGACGCAGCC CAGCATCTTC TCAGAGGTGA AGAAGCAGCT GCATGATCTG 
TTTGCCATCG AAGAGAGCGA GGACGAGACG GTGAACGCTG CCCTGGCTGC CGTGGGTAAG 
CGTCGCCAGG AGTACACGAG CATCAAGCGC AGCACTACTC CAGCCACGTC CACGGACAAA 
ACCACTCTCT CATCAGCGGA CGATGAGCTG ACAGCCACCA CCACCACAGC AACAGAAGGT 
GCTTTGGAGG GTAGCAAAGA TAACTTCCAC AAGGACCTCA TGGAGCATGT GGTTTATGCC 
ACATCCACAT CGACCAAAGT CACCTCCGAG ACAGAGATCT GCTATCGCGG ACGCTGCATA 
AGGTCCGAGG ATCTGCCCGC CAACCACAAA CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039306.1
CDS	407..5185
Translation	MKRGMQLTAIGLCLLATAATVVRAQESSDKWSRQLEDAPTSDTDSLDWIPLAQPLDGSGGKDRSGNGRVLTYSQTFPPSARFGDLNKPGVSPGSQFDFSYQFPRYSNGQGQALPLRQGPPSSLKTNSGQEPTSQSFFKFEMPFHSNEGGQAQAPPTLQTGYQIQHTFGGGLQAPSSLFSPPSLFGQQQAQPFGSEFHTLPQKPLAQTLNKPLRQQNYIQDILPPKSHAKPTQPSLQSVTPQVFPLDPEPESIFGLNKPAPSSAPASSGGRQQEVQLLYVPYDTLYNQQRQQSGSRPGSSNSNFEVNKFNVNPGLPAVNPYQINQFYTQDPNVGSDFFSAGTTSRPSTTTSQPHSIFQSFGQSQPQPQPQAQTQFSTAKPKPKAHQPPLAMFLLRSSSRPSQSDVVAALRSAHSISVIDSPTKQTPEIFVGPAGMPIPDGYVKFDLPYLSQLAQTRDLSDVSFFVAPLSYRTPNGFNKILLPEPHVGSIVVNRAKDSVSVQTQAPPQRQQRPYAAVSSTRNPFETTTQAQAQSPLQSQSQGASGAPVRGNKFSYYYVQDGIQEVSSPKIPAKQERHKKKRPQNVQVEHQHHQQQQQQPIYRPPTKTQNPFDTLNQIGGDDFFKSLTNKPTTTSSTSTSTTSTTTSTTSTTAAPTQPLFTYSTRPQVEFPGSSGHLYPEYLQPVVQEQQPRLTTRPPTATTVEEEHRMKQYFRQQDAFRQRPLTTIPPAFEQVQYTPSAPDYEVPTTQRVKSKHRVNIYTPAAVPVTTSDVGYQVDQEQAQAPLNHRPSFNQVQNEILDNSNVDYEPNPYHVPSELPALTPDIPGLVNNLQEKDKLQPGTTITPQSEPEAETRPTRRPLLRTRKPAVSKVVTTSSVSYSESESSGKVPHRIRRPYGNRGTAAGTASTTGGGGDQGDDSAPIPTRRPTSARNPLIRNPNRIRYRPTTEERQSLKVKTRKGSKNGQREDQDLDYQRDVLKQNYPVFKGSSSAPSRRPTSPTAIPSSYDFQATTEGPASESQQVYTVTPSNSIDSASVQGSGFPSSLLEPMQIAQHYQEFSKDNYGPGYFPNQEDLTPTEAIVRNEVSPIITTLATTTPSTTTTSTTTTTEAAPTTTTRRSPFVRRNYPRVRTTTTTEPPVTSTTPSEERPSIRTRLPPRRVVKVRQRQRRPGHPASSTAAPEEEVQEAATQRTNLRKLSRYNQEPKEKEQTTITQRRRYKQPFQLEGQESQWSPSSETSNSNNNANANTNSFKPLNPKYKTESHNFENEPEIVTVGPTAQPETYEFNVAADLAGSAVQRTTTLKAPNLKPEKSFAELLEEVMGKVPEELATESPNTSSTIGRLNRRGKWKKSRVTSGSENGENFETAESQNLGPQLYNALQAASEKEEQQPKTTTPLTPAATEAETAETIVPTTAASPTTPEEYEVTTARALEEATLASTLPMETLESATRRMDTAADVDVDGDGDLETQPSIFSEVKKQLHDLFAIEESEDETVNAALAAVGKRRQEYTSIKRSTTPATSTDKTTLSSADDELTATTTTATEGALEGSKDNFHKDLMEHVVYATSTSTKVTSETEICYRGRCIRSEDLPANHKLH

Target ORF information:

RefSeq Version	XM_015183820.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183820.1

ORF Insert Sequence:

ATGAAGCGAG GCATGCAACT TACCGCCATT GGGCTGTGCC TATTGGCCAC AGCTGCCACC 
GTTGTCCGGG CCCAGGAATC ATCGGACAAG TGGTCGCGGC AGCTCGAGGA TGCTCCCACC 
TCCGACACGG ATTCCCTGGA TTGGATACCA CTCGCCCAGC CGCTGGACGG AAGCGGCGGA 
AAGGATCGTT CCGGCAACGG TCGTGTGCTG ACCTACTCGC AGACGTTTCC ACCCAGCGCC 
CGTTTCGGGG ACCTGAACAA GCCAGGAGTA TCCCCCGGAT CGCAGTTTGA TTTTTCGTAC 
CAGTTTCCAA GGTACTCGAA TGGACAGGGA CAAGCCCTGC CCCTGCGACA GGGGCCACCG 
TCATCGCTGA AGACGAACTC CGGCCAGGAG CCCACATCGC AGTCGTTCTT CAAGTTTGAG 
ATGCCCTTCC ACAGTAACGA GGGGGGACAG GCCCAGGCGC CGCCCACTCT GCAGACGGGC 
TATCAGATCC AGCACACCTT TGGAGGCGGT CTTCAGGCCC CATCTTCGCT CTTCAGTCCT 
CCCTCACTGT TTGGCCAGCA GCAGGCGCAG CCCTTCGGCT CCGAGTTCCA TACCCTGCCG 
CAGAAGCCAC TGGCACAGAC CCTGAACAAG CCCCTCCGGC AGCAAAACTA CATCCAGGAC 
ATCCTGCCGC CAAAGTCCCA TGCGAAGCCG ACGCAGCCAT CGCTGCAGAG CGTGACCCCC 
CAGGTCTTTC CCCTCGATCC CGAGCCAGAG TCGATCTTTG GGTTGAACAA GCCGGCGCCC 
TCGTCCGCTC CGGCCTCGAG TGGAGGCCGG CAGCAGGAGG TCCAGCTGCT GTACGTGCCC 
TACGACACTC TGTACAACCA GCAGCGACAG CAGTCGGGCT CCAGGCCAGG ATCTTCCAAC 
TCCAACTTTG AGGTTAACAA GTTCAATGTG AATCCAGGAC TTCCTGCCGT GAATCCCTAC 
CAGATCAACC AGTTCTACAC CCAGGATCCC AACGTGGGCA GCGACTTCTT CAGCGCAGGA 
ACCACCTCTC GACCGAGCAC CACCACCAGC CAGCCGCATA GCATCTTCCA GAGCTTTGGC 
CAGTCCCAGC CGCAGCCACA ACCGCAGGCA CAGACGCAGT TCTCCACCGC CAAGCCCAAG 
CCGAAGGCTC ACCAGCCCCC GCTGGCGATG TTCCTGCTGC GCTCCAGCTC CCGGCCATCG 
CAGTCGGATG TGGTGGCTGC CCTGCGCAGT GCCCACAGCA TTTCCGTGAT CGATTCCCCA 
ACGAAACAGA CGCCGGAGAT CTTTGTGGGA CCCGCAGGAA TGCCCATTCC CGATGGCTAT 
GTGAAGTTTG ATCTTCCGTA TTTGTCGCAA CTGGCGCAGA CCCGCGACCT CAGCGATGTC 
TCCTTCTTCG TGGCTCCGCT CAGCTATCGT ACACCGAATG GATTCAACAA GATCCTCCTG 
CCCGAGCCTC ATGTGGGTTC CATTGTGGTC AACCGCGCCA AGGACTCTGT TTCGGTGCAG 
ACTCAGGCAC CGCCACAGCG TCAGCAGCGT CCCTATGCAG CCGTGAGCAG CACGCGGAAT 
CCCTTCGAGA CCACAACCCA GGCACAGGCA CAGTCCCCGT TGCAGTCGCA GTCACAGGGT 
GCATCGGGTG CACCTGTACG CGGGAACAAG TTCAGCTACT ATTATGTGCA GGACGGCATC 
CAGGAGGTGA GTTCTCCGAA GATTCCCGCC AAGCAGGAGC GCCACAAGAA GAAGCGACCC 
CAGAACGTTC AGGTGGAGCA TCAGCATCAT CAGCAGCAGC AGCAGCAGCC CATCTACCGT 
CCACCCACCA AGACGCAGAA CCCCTTCGAC ACGCTCAATC AGATCGGAGG CGATGACTTC 
TTCAAGTCGC TAACCAACAA GCCCACTACT ACCAGCTCCA CATCCACCAG CACAACATCG 
ACCACAACCT CGACGACATC CACCACGGCG GCGCCCACCC AGCCCCTCTT TACGTACAGC 
ACCCGGCCGC AGGTGGAATT CCCCGGCTCC AGTGGCCACC TGTATCCCGA GTACCTGCAG 
CCCGTCGTCC AGGAACAGCA GCCCCGTCTG ACCACGAGAC CGCCGACGGC CACCACAGTG 
GAAGAAGAGC ATCGCATGAA GCAGTACTTC CGGCAGCAAG ATGCCTTCCG CCAGCGTCCG 
CTCACTACGA TTCCGCCCGC CTTCGAACAG GTTCAGTACA CGCCCAGTGC ACCCGACTAC 
GAGGTGCCTA CGACGCAGAG GGTCAAGAGC AAGCATCGCG TGAACATCTA TACGCCTGCA 
GCAGTGCCGG TTACCACCTC GGATGTGGGC TACCAAGTGG ACCAAGAGCA GGCGCAGGCG 
CCGCTGAATC ACAGGCCCAG CTTCAACCAA GTGCAGAACG AGATTCTGGA TAACAGCAAC 
GTGGATTACG AGCCGAATCC CTACCATGTG CCGTCGGAGC TGCCGGCCTT GACGCCCGAC 
ATTCCCGGAT TGGTCAATAA TCTCCAGGAG AAAGACAAGC TCCAGCCAGG AACAACCATC 
ACGCCACAGA GCGAGCCCGA GGCGGAAACG CGACCCACCC GCCGTCCCCT GCTGCGCACC 
CGCAAGCCAG CCGTTTCCAA GGTGGTGACC ACCTCCTCCG TGTCGTACTC CGAGTCCGAG 
TCAAGCGGAA AGGTGCCGCA TCGCATCCGT CGACCATACG GTAACCGAGG CACTGCAGCC 
GGAACCGCCT CCACCACGGG CGGCGGCGGA GACCAGGGAG ACGACTCTGC ACCGATCCCC 
ACCCGGCGAC CAACCAGCGC CCGGAATCCC CTCATTCGGA ATCCAAATCG TATCCGCTAC 
AGGCCCACCA CCGAGGAGCG CCAGTCGCTG AAGGTGAAGA CCAGGAAGGG ATCAAAGAAC 
GGCCAACGCG AAGACCAGGA TCTCGACTAC CAACGTGATG TGCTCAAGCA GAACTATCCG 
GTGTTCAAGG GCTCCTCCTC CGCCCCCTCG CGCCGACCCA CAAGCCCCAC CGCCATTCCC 
AGTTCGTACG ACTTCCAGGC CACCACCGAA GGCCCGGCTT CGGAGTCACA GCAGGTGTAC 
ACGGTGACGC CCAGCAACAG CATCGACAGC GCCAGCGTTC AGGGTTCGGG ATTCCCGTCC 
AGCCTGCTGG AGCCCATGCA GATCGCCCAG CATTACCAGG AGTTCTCCAA GGACAACTAC 
GGGCCCGGCT ACTTCCCCAA TCAGGAGGAT CTCACACCCA CCGAGGCCAT TGTTCGCAAT 
GAGGTGAGTC CCATTATCAC CACGCTGGCT ACCACAACGC CCTCGACAAC TACGACCAGC 
ACCACGACAA CCACAGAGGC AGCGCCCACG ACCACAACAC GTCGCTCGCC GTTCGTGCGC 
CGAAACTATC CCAGGGTGAG GACTACCACC ACAACAGAGC CGCCAGTGAC CAGCACCACG 
CCCTCGGAGG AGCGTCCTTC CATTCGCACT CGGCTGCCGC CTCGTCGTGT GGTCAAGGTG 
CGACAACGTC AGCGTCGTCC AGGACACCCT GCCTCCTCCA CTGCGGCCCC CGAAGAGGAA 
GTCCAGGAAG CAGCAACCCA GAGGACAAAT CTCCGCAAGC TGAGCCGCTA CAATCAAGAG 
CCCAAAGAAA AAGAGCAGAC AACCATCACC CAGCGGCGTC GCTATAAGCA GCCATTCCAG 
CTGGAGGGCC AAGAGTCCCA GTGGTCGCCA TCCTCGGAGA CTTCCAACAG CAACAACAAT 
GCCAATGCCA ACACCAACAG TTTCAAGCCA CTGAATCCCA AGTACAAGAC AGAGTCCCAC 
AACTTCGAGA ACGAGCCAGA GATCGTGACT GTGGGACCCA CAGCGCAGCC CGAGACGTAC 
GAGTTCAACG TAGCTGCGGA TCTGGCTGGA TCAGCCGTGC AACGCACCAC CACCCTAAAG 
GCGCCAAACC TGAAACCAGA GAAATCCTTT GCTGAACTGC TCGAGGAAGT GATGGGCAAG 
GTTCCCGAGG AACTGGCCAC AGAGTCACCC AACACCAGCA GCACCATTGG CAGGCTGAAT 
AGGCGTGGCA AGTGGAAGAA GAGCCGCGTG ACTAGTGGAT CGGAAAATGG CGAAAACTTC 
GAGACGGCCG AGTCCCAGAA TCTGGGACCT CAGCTCTACA ATGCCCTGCA GGCGGCCAGC 
GAGAAGGAGG AGCAGCAGCC GAAGACCACC ACCCCGCTGA CACCTGCAGC CACCGAGGCT 
GAGACAGCAG AAACGATAGT CCCCACCACG GCTGCCTCGC CCACGACACC AGAGGAGTAT 
GAGGTGACCA CCGCCAGAGC CTTGGAGGAG GCAACACTGG CATCGACGCT GCCAATGGAG 
ACGCTGGAGA GTGCCACGCG TCGCATGGAT ACCGCGGCGG ATGTGGATGT GGATGGGGAT 
GGCGACCTGG AGACGCAGCC CAGCATCTTC TCAGAGGTGA AGAAGCAGCT GCATGATCTG 
TTTGCCATCG AAGAGAGCGA GGACGAGACG GTGAACGCTG CCCTGGCTGC CGTGGGTAAG 
CGTCGCCAGG AGTACACGAG CATCAAGCGC AGCACTACTC CAGCCACGTC CACGGACAAA 
ACCACTCTCT CATCAGCGGA CGATGAGCTG ACAGCCACCA CCACCACAGC AACAGAAGGT 
GCTTTGGAGG GTAGCAAAGA TAACTTCCAC AAGGACCTCA TGGAGCATGT GGTTTATGCC 
ACATCCACAT CGACCAAAGT CACCTCCGAG ACAGAGATCT GCTATCGCGG ACGCTGCATA 
AGGTCCGAGG ATCTGCCCGC CAACCACAAA CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA13474 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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