LOC103643560 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103643560
Entrez Gene ID	103643560
Full Name	protein NETWORKED 1D
Synonyms	GRMZM2G324390
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_020546786.1	XP_020402375.1	protein NETWORKED 1D
XM_020546787.1	XP_020402376.1	protein NETWORKED 1D
XM_008666729.2	XP_008664951.2	protein NETWORKED 1D
XM_020546788.1	XP_020402377.1	protein NETWORKED 1D
XM_020546785.1	XP_020402374.1	protein NETWORKED 1D

Pathways from BioSystems
Gene Ontology

The following LOC103643560 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103643560 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa55760	XM_020546787.1 Latest version!	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OZa55760	XM_020546786.1 Latest version!	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OZa55760	XM_020546785.1 Latest version!	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OZa55760	XM_008666729.2 Latest version!	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OZa55760	XM_020546788.1 Latest version!	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103643560 ( XM_020546786.1 ) cDNA ORF clone, Zea mays -> XP_020402375.1 Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X2, mRNA.

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CloneID	OZa55760
Clone ID Related Accession (Same CDS sequence)	XM_020546786.1 , XM_020546787.1 , XM_008666729.2 , XM_020546788.1 , XM_020546785.1
Accession Version	XM_020546786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	protein NETWORKED 1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020402375.1
CDS	400..8118
Translation	MATLVRHDSRQYSWWISHISPKNSKWLQENVSDMDSKVKAMIKLINEDADSFAKRAEMYYKKRPELMNLVEEFYRAYRALAERYDQSTGALRQAHRTISEAFPNQMPSMSDESPSAFSQEMEPHTPDTSTLTRVAFDYDDLQKDGVGVSPQSFTSKRNGTHPEETSALSSSVENAPRAGFDGKVWKGLTFEGPNVKGKDDISNEMANLQQEVARLLAESQDLKQQMFSESERANKAENEMQMLKATVLQLNAEKDTSLMQYNNSSERISTLESELSKAQSDLEKLTDEMAADVQKLINAETLNIAIQSEAEGLDQKMKMQQQALEQKLKELESFRSSFQEEHEKRMQAESALLSQRKELAQSHEEVQRLAIEIKTANEKLNEFKQTKEVLEYTVCELKKDVERLTEQNQSSEVLVQKLGDQINTLKDSNNELQSEIQSLKSIISQLNTEKNAAVLQHQQFVELVSVLESQLSKLQSELDETEQKVQMLTHDLEKKKEEADNVHLKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDGSTKKLSELEKNKLDLESILKELNNTILGLNSEKDATLLQQQQSLQKVSDLELELSKMQLEMEVLEDTVCELKKDVERHTEQNQSSEVLIQKLGEEINTIKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSIELVSVLESQLSKLHSELDEIEQKVQLLTHDLEKKKEEADNVHIKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDRSTKKLSELENNKLDLESILKELNKTILGLNSEKDAALLQQQQYLQKVSDLELELSKMQLEMEVLEDTVCDLKKDVERHTEQNQSSEVLIQKLGEEINTLKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSVELVSVLESQLSKLQSELDETEQRVQLLTQDLEKKKEEADNVHLKLQDECHRRMQIEAALLMTEGLHSQLQEKMQMLTQDFDGSTKKLSELENNKLDLESTLKELNNTILGLNSEKDAALLQQQQSLQKVSNLELELSMMQLEMDKSEQKILLLEQEIARKNGSVDSLEISLKDECEKRLQAQKMYSQSQEDVSRLYIEIEKQNGKLNELENLISELNNTILLINTEKDATLHENQQSSVRISDLESELMALKTELENVEGKVHAVEQELIYKKEEADNLQISLQDEIQKRVEGEESLLMMTNLHSGSQNEVRGLALELEKLNGNLRQVENSKVDLENIVTKHTEEIHILRERNLSTDLMIKDLHLELDALKDLNVKLQTEMGLHIGEKEALQREFTSQREEKKNLEGIHHTLVDEMSTLKTTATMNQKLIEELQITNSKLKEVCAKSEVEKALLSEKLQEVEKLSEECSLLENSLSDANAEMDALRDKIKALEALESSLKDIISCHVSEKAVLVSELEILGKRLSDASEKNSIMDSSLFDMKMDLEDLRTKLKDSEESCQALLANNSALSGEVDALREKIKALQDSESSLKDAISCYVSEKAVLASELEILGKSLSEVSEKNSILDISLCDMKTELDELRTKLKDSEESCQAHLTNNSALSAEKNNLFSQLESITLAMKALEGMHANLEQKHSSVSREKDFAYDQVRELQDQLRIKNEEFEVSAKSHQLQANSYEKQIFSLQEKNHYMEEVLQQEQQKNINASISTVILENCLVDEQDKKVALFTECQKYAVENHSANMLVSELMEEARYHGEERKKLLAHNEKLREGISNQMKFLNICKDLGPADLAEDEVLLQTVSDETINIMRLKDETEDVNRLMDTELSVLSVVLLQVGMELRDLHLQKCALEKEVESGEKETLSLQNQNQQMLEQNEQLRSGLQESSEGVEVLKTEVFVIQEKLSCLRESYQTSQDEISNLTKKNESLSKEYQSLSEKYNALEDENDTIFEECMMLKNLCLFFRGHNNEIASALASLTDEMALLSLAKGDLDLQVNELNRRSVMLESENYHLKDYIIYLVEILRTRLVLSEFDLDTNRSVFQELVVELENCMTQLVQKDDELMEAEEKVQLLQEKNRELCGVVGSLQVAMEGAKVVKGELEKKITILIEQCSSKDDKILLLHQDNEALQSEIEQCEREFVELMDDAITFSVNSAVYEEKAFELMMNGKATENRAISQKELLMKEISSRDANIEELQKRLAGIQDEHAELKAELNTHLALIASLVDHVSVLEEDVRSLSKPCSTEGKEETARVHHVQEGDDGLESHCLVNGTPKLQGLIARIEALQVVVLNAKDRQDRKSAESAAKLAAASAEIQELKTRGGSRMEAKEICSDNEKQDVEVSKGKQVQIMKDIELDQISTCPPYGAGAALYPHGTGANAELDDDMLQLWEAAERSCKNQTAKSSSSEHDIQAVEDLESECPSSELARGRDLGTNKLEVPKGAVEPHEAWSKNVLERLTSDAQRLLSIQASIEELKKKMEGSSKGTNSEYGSVSAQVHETEGLVLEQINSNNKLSRKAENYPALSDNTSTERGGYSSRRKISEQVQRGSENVARLELELQKIQYVLLKLEEEHEYTRLKVSDKRTRVLLRDYLYGRKDRGGGQKGKKKRVPFCGCVRSKSRTEP

Target ORF information:

RefSeq Version	XM_020546786.1
Organism	Zea mays
Definition	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020546786.1

ORF Insert Sequence:

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103643560 ( XM_020546787.1 ) cDNA ORF clone, Zea mays -> XP_020402376.1 Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X3, mRNA.

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CloneID	OZa55760
Clone ID Related Accession (Same CDS sequence)	XM_020546786.1 , XM_020546787.1 , XM_008666729.2 , XM_020546788.1 , XM_020546785.1
Accession Version	XM_020546787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	protein NETWORKED 1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020402376.1
CDS	392..8110
Translation	MATLVRHDSRQYSWWISHISPKNSKWLQENVSDMDSKVKAMIKLINEDADSFAKRAEMYYKKRPELMNLVEEFYRAYRALAERYDQSTGALRQAHRTISEAFPNQMPSMSDESPSAFSQEMEPHTPDTSTLTRVAFDYDDLQKDGVGVSPQSFTSKRNGTHPEETSALSSSVENAPRAGFDGKVWKGLTFEGPNVKGKDDISNEMANLQQEVARLLAESQDLKQQMFSESERANKAENEMQMLKATVLQLNAEKDTSLMQYNNSSERISTLESELSKAQSDLEKLTDEMAADVQKLINAETLNIAIQSEAEGLDQKMKMQQQALEQKLKELESFRSSFQEEHEKRMQAESALLSQRKELAQSHEEVQRLAIEIKTANEKLNEFKQTKEVLEYTVCELKKDVERLTEQNQSSEVLVQKLGDQINTLKDSNNELQSEIQSLKSIISQLNTEKNAAVLQHQQFVELVSVLESQLSKLQSELDETEQKVQMLTHDLEKKKEEADNVHLKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDGSTKKLSELEKNKLDLESILKELNNTILGLNSEKDATLLQQQQSLQKVSDLELELSKMQLEMEVLEDTVCELKKDVERHTEQNQSSEVLIQKLGEEINTIKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSIELVSVLESQLSKLHSELDEIEQKVQLLTHDLEKKKEEADNVHIKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDRSTKKLSELENNKLDLESILKELNKTILGLNSEKDAALLQQQQYLQKVSDLELELSKMQLEMEVLEDTVCDLKKDVERHTEQNQSSEVLIQKLGEEINTLKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSVELVSVLESQLSKLQSELDETEQRVQLLTQDLEKKKEEADNVHLKLQDECHRRMQIEAALLMTEGLHSQLQEKMQMLTQDFDGSTKKLSELENNKLDLESTLKELNNTILGLNSEKDAALLQQQQSLQKVSNLELELSMMQLEMDKSEQKILLLEQEIARKNGSVDSLEISLKDECEKRLQAQKMYSQSQEDVSRLYIEIEKQNGKLNELENLISELNNTILLINTEKDATLHENQQSSVRISDLESELMALKTELENVEGKVHAVEQELIYKKEEADNLQISLQDEIQKRVEGEESLLMMTNLHSGSQNEVRGLALELEKLNGNLRQVENSKVDLENIVTKHTEEIHILRERNLSTDLMIKDLHLELDALKDLNVKLQTEMGLHIGEKEALQREFTSQREEKKNLEGIHHTLVDEMSTLKTTATMNQKLIEELQITNSKLKEVCAKSEVEKALLSEKLQEVEKLSEECSLLENSLSDANAEMDALRDKIKALEALESSLKDIISCHVSEKAVLVSELEILGKRLSDASEKNSIMDSSLFDMKMDLEDLRTKLKDSEESCQALLANNSALSGEVDALREKIKALQDSESSLKDAISCYVSEKAVLASELEILGKSLSEVSEKNSILDISLCDMKTELDELRTKLKDSEESCQAHLTNNSALSAEKNNLFSQLESITLAMKALEGMHANLEQKHSSVSREKDFAYDQVRELQDQLRIKNEEFEVSAKSHQLQANSYEKQIFSLQEKNHYMEEVLQQEQQKNINASISTVILENCLVDEQDKKVALFTECQKYAVENHSANMLVSELMEEARYHGEERKKLLAHNEKLREGISNQMKFLNICKDLGPADLAEDEVLLQTVSDETINIMRLKDETEDVNRLMDTELSVLSVVLLQVGMELRDLHLQKCALEKEVESGEKETLSLQNQNQQMLEQNEQLRSGLQESSEGVEVLKTEVFVIQEKLSCLRESYQTSQDEISNLTKKNESLSKEYQSLSEKYNALEDENDTIFEECMMLKNLCLFFRGHNNEIASALASLTDEMALLSLAKGDLDLQVNELNRRSVMLESENYHLKDYIIYLVEILRTRLVLSEFDLDTNRSVFQELVVELENCMTQLVQKDDELMEAEEKVQLLQEKNRELCGVVGSLQVAMEGAKVVKGELEKKITILIEQCSSKDDKILLLHQDNEALQSEIEQCEREFVELMDDAITFSVNSAVYEEKAFELMMNGKATENRAISQKELLMKEISSRDANIEELQKRLAGIQDEHAELKAELNTHLALIASLVDHVSVLEEDVRSLSKPCSTEGKEETARVHHVQEGDDGLESHCLVNGTPKLQGLIARIEALQVVVLNAKDRQDRKSAESAAKLAAASAEIQELKTRGGSRMEAKEICSDNEKQDVEVSKGKQVQIMKDIELDQISTCPPYGAGAALYPHGTGANAELDDDMLQLWEAAERSCKNQTAKSSSSEHDIQAVEDLESECPSSELARGRDLGTNKLEVPKGAVEPHEAWSKNVLERLTSDAQRLLSIQASIEELKKKMEGSSKGTNSEYGSVSAQVHETEGLVLEQINSNNKLSRKAENYPALSDNTSTERGGYSSRRKISEQVQRGSENVARLELELQKIQYVLLKLEEEHEYTRLKVSDKRTRVLLRDYLYGRKDRGGGQKGKKKRVPFCGCVRSKSRTEP

Target ORF information:

RefSeq Version	XM_020546787.1
Organism	Zea mays
Definition	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020546787.1

ORF Insert Sequence:

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103643560 ( XM_008666729.2 ) cDNA ORF clone, Zea mays -> XP_008664951.2 Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X5, mRNA.

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CloneID	OZa55760
Clone ID Related Accession (Same CDS sequence)	XM_020546786.1 , XM_020546787.1 , XM_008666729.2 , XM_020546788.1 , XM_020546785.1
Accession Version	XM_008666729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	protein NETWORKED 1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008666729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008664951.2
CDS	298..8016
Translation	MATLVRHDSRQYSWWISHISPKNSKWLQENVSDMDSKVKAMIKLINEDADSFAKRAEMYYKKRPELMNLVEEFYRAYRALAERYDQSTGALRQAHRTISEAFPNQMPSMSDESPSAFSQEMEPHTPDTSTLTRVAFDYDDLQKDGVGVSPQSFTSKRNGTHPEETSALSSSVENAPRAGFDGKVWKGLTFEGPNVKGKDDISNEMANLQQEVARLLAESQDLKQQMFSESERANKAENEMQMLKATVLQLNAEKDTSLMQYNNSSERISTLESELSKAQSDLEKLTDEMAADVQKLINAETLNIAIQSEAEGLDQKMKMQQQALEQKLKELESFRSSFQEEHEKRMQAESALLSQRKELAQSHEEVQRLAIEIKTANEKLNEFKQTKEVLEYTVCELKKDVERLTEQNQSSEVLVQKLGDQINTLKDSNNELQSEIQSLKSIISQLNTEKNAAVLQHQQFVELVSVLESQLSKLQSELDETEQKVQMLTHDLEKKKEEADNVHLKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDGSTKKLSELEKNKLDLESILKELNNTILGLNSEKDATLLQQQQSLQKVSDLELELSKMQLEMEVLEDTVCELKKDVERHTEQNQSSEVLIQKLGEEINTIKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSIELVSVLESQLSKLHSELDEIEQKVQLLTHDLEKKKEEADNVHIKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDRSTKKLSELENNKLDLESILKELNKTILGLNSEKDAALLQQQQYLQKVSDLELELSKMQLEMEVLEDTVCDLKKDVERHTEQNQSSEVLIQKLGEEINTLKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSVELVSVLESQLSKLQSELDETEQRVQLLTQDLEKKKEEADNVHLKLQDECHRRMQIEAALLMTEGLHSQLQEKMQMLTQDFDGSTKKLSELENNKLDLESTLKELNNTILGLNSEKDAALLQQQQSLQKVSNLELELSMMQLEMDKSEQKILLLEQEIARKNGSVDSLEISLKDECEKRLQAQKMYSQSQEDVSRLYIEIEKQNGKLNELENLISELNNTILLINTEKDATLHENQQSSVRISDLESELMALKTELENVEGKVHAVEQELIYKKEEADNLQISLQDEIQKRVEGEESLLMMTNLHSGSQNEVRGLALELEKLNGNLRQVENSKVDLENIVTKHTEEIHILRERNLSTDLMIKDLHLELDALKDLNVKLQTEMGLHIGEKEALQREFTSQREEKKNLEGIHHTLVDEMSTLKTTATMNQKLIEELQITNSKLKEVCAKSEVEKALLSEKLQEVEKLSEECSLLENSLSDANAEMDALRDKIKALEALESSLKDIISCHVSEKAVLVSELEILGKRLSDASEKNSIMDSSLFDMKMDLEDLRTKLKDSEESCQALLANNSALSGEVDALREKIKALQDSESSLKDAISCYVSEKAVLASELEILGKSLSEVSEKNSILDISLCDMKTELDELRTKLKDSEESCQAHLTNNSALSAEKNNLFSQLESITLAMKALEGMHANLEQKHSSVSREKDFAYDQVRELQDQLRIKNEEFEVSAKSHQLQANSYEKQIFSLQEKNHYMEEVLQQEQQKNINASISTVILENCLVDEQDKKVALFTECQKYAVENHSANMLVSELMEEARYHGEERKKLLAHNEKLREGISNQMKFLNICKDLGPADLAEDEVLLQTVSDETINIMRLKDETEDVNRLMDTELSVLSVVLLQVGMELRDLHLQKCALEKEVESGEKETLSLQNQNQQMLEQNEQLRSGLQESSEGVEVLKTEVFVIQEKLSCLRESYQTSQDEISNLTKKNESLSKEYQSLSEKYNALEDENDTIFEECMMLKNLCLFFRGHNNEIASALASLTDEMALLSLAKGDLDLQVNELNRRSVMLESENYHLKDYIIYLVEILRTRLVLSEFDLDTNRSVFQELVVELENCMTQLVQKDDELMEAEEKVQLLQEKNRELCGVVGSLQVAMEGAKVVKGELEKKITILIEQCSSKDDKILLLHQDNEALQSEIEQCEREFVELMDDAITFSVNSAVYEEKAFELMMNGKATENRAISQKELLMKEISSRDANIEELQKRLAGIQDEHAELKAELNTHLALIASLVDHVSVLEEDVRSLSKPCSTEGKEETARVHHVQEGDDGLESHCLVNGTPKLQGLIARIEALQVVVLNAKDRQDRKSAESAAKLAAASAEIQELKTRGGSRMEAKEICSDNEKQDVEVSKGKQVQIMKDIELDQISTCPPYGAGAALYPHGTGANAELDDDMLQLWEAAERSCKNQTAKSSSSEHDIQAVEDLESECPSSELARGRDLGTNKLEVPKGAVEPHEAWSKNVLERLTSDAQRLLSIQASIEELKKKMEGSSKGTNSEYGSVSAQVHETEGLVLEQINSNNKLSRKAENYPALSDNTSTERGGYSSRRKISEQVQRGSENVARLELELQKIQYVLLKLEEEHEYTRLKVSDKRTRVLLRDYLYGRKDRGGGQKGKKKRVPFCGCVRSKSRTEP

Target ORF information:

RefSeq Version	XM_008666729.2
Organism	Zea mays
Definition	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008666729.2

ORF Insert Sequence:

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103643560 ( XM_020546788.1 ) cDNA ORF clone, Zea mays -> XP_020402377.1 Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X4, mRNA.

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CloneID	OZa55760
Clone ID Related Accession (Same CDS sequence)	XM_020546786.1 , XM_020546787.1 , XM_008666729.2 , XM_020546788.1 , XM_020546785.1
Accession Version	XM_020546788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	protein NETWORKED 1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020402377.1
CDS	438..8156
Translation	MATLVRHDSRQYSWWISHISPKNSKWLQENVSDMDSKVKAMIKLINEDADSFAKRAEMYYKKRPELMNLVEEFYRAYRALAERYDQSTGALRQAHRTISEAFPNQMPSMSDESPSAFSQEMEPHTPDTSTLTRVAFDYDDLQKDGVGVSPQSFTSKRNGTHPEETSALSSSVENAPRAGFDGKVWKGLTFEGPNVKGKDDISNEMANLQQEVARLLAESQDLKQQMFSESERANKAENEMQMLKATVLQLNAEKDTSLMQYNNSSERISTLESELSKAQSDLEKLTDEMAADVQKLINAETLNIAIQSEAEGLDQKMKMQQQALEQKLKELESFRSSFQEEHEKRMQAESALLSQRKELAQSHEEVQRLAIEIKTANEKLNEFKQTKEVLEYTVCELKKDVERLTEQNQSSEVLVQKLGDQINTLKDSNNELQSEIQSLKSIISQLNTEKNAAVLQHQQFVELVSVLESQLSKLQSELDETEQKVQMLTHDLEKKKEEADNVHLKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDGSTKKLSELEKNKLDLESILKELNNTILGLNSEKDATLLQQQQSLQKVSDLELELSKMQLEMEVLEDTVCELKKDVERHTEQNQSSEVLIQKLGEEINTIKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSIELVSVLESQLSKLHSELDEIEQKVQLLTHDLEKKKEEADNVHIKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDRSTKKLSELENNKLDLESILKELNKTILGLNSEKDAALLQQQQYLQKVSDLELELSKMQLEMEVLEDTVCDLKKDVERHTEQNQSSEVLIQKLGEEINTLKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSVELVSVLESQLSKLQSELDETEQRVQLLTQDLEKKKEEADNVHLKLQDECHRRMQIEAALLMTEGLHSQLQEKMQMLTQDFDGSTKKLSELENNKLDLESTLKELNNTILGLNSEKDAALLQQQQSLQKVSNLELELSMMQLEMDKSEQKILLLEQEIARKNGSVDSLEISLKDECEKRLQAQKMYSQSQEDVSRLYIEIEKQNGKLNELENLISELNNTILLINTEKDATLHENQQSSVRISDLESELMALKTELENVEGKVHAVEQELIYKKEEADNLQISLQDEIQKRVEGEESLLMMTNLHSGSQNEVRGLALELEKLNGNLRQVENSKVDLENIVTKHTEEIHILRERNLSTDLMIKDLHLELDALKDLNVKLQTEMGLHIGEKEALQREFTSQREEKKNLEGIHHTLVDEMSTLKTTATMNQKLIEELQITNSKLKEVCAKSEVEKALLSEKLQEVEKLSEECSLLENSLSDANAEMDALRDKIKALEALESSLKDIISCHVSEKAVLVSELEILGKRLSDASEKNSIMDSSLFDMKMDLEDLRTKLKDSEESCQALLANNSALSGEVDALREKIKALQDSESSLKDAISCYVSEKAVLASELEILGKSLSEVSEKNSILDISLCDMKTELDELRTKLKDSEESCQAHLTNNSALSAEKNNLFSQLESITLAMKALEGMHANLEQKHSSVSREKDFAYDQVRELQDQLRIKNEEFEVSAKSHQLQANSYEKQIFSLQEKNHYMEEVLQQEQQKNINASISTVILENCLVDEQDKKVALFTECQKYAVENHSANMLVSELMEEARYHGEERKKLLAHNEKLREGISNQMKFLNICKDLGPADLAEDEVLLQTVSDETINIMRLKDETEDVNRLMDTELSVLSVVLLQVGMELRDLHLQKCALEKEVESGEKETLSLQNQNQQMLEQNEQLRSGLQESSEGVEVLKTEVFVIQEKLSCLRESYQTSQDEISNLTKKNESLSKEYQSLSEKYNALEDENDTIFEECMMLKNLCLFFRGHNNEIASALASLTDEMALLSLAKGDLDLQVNELNRRSVMLESENYHLKDYIIYLVEILRTRLVLSEFDLDTNRSVFQELVVELENCMTQLVQKDDELMEAEEKVQLLQEKNRELCGVVGSLQVAMEGAKVVKGELEKKITILIEQCSSKDDKILLLHQDNEALQSEIEQCEREFVELMDDAITFSVNSAVYEEKAFELMMNGKATENRAISQKELLMKEISSRDANIEELQKRLAGIQDEHAELKAELNTHLALIASLVDHVSVLEEDVRSLSKPCSTEGKEETARVHHVQEGDDGLESHCLVNGTPKLQGLIARIEALQVVVLNAKDRQDRKSAESAAKLAAASAEIQELKTRGGSRMEAKEICSDNEKQDVEVSKGKQVQIMKDIELDQISTCPPYGAGAALYPHGTGANAELDDDMLQLWEAAERSCKNQTAKSSSSEHDIQAVEDLESECPSSELARGRDLGTNKLEVPKGAVEPHEAWSKNVLERLTSDAQRLLSIQASIEELKKKMEGSSKGTNSEYGSVSAQVHETEGLVLEQINSNNKLSRKAENYPALSDNTSTERGGYSSRRKISEQVQRGSENVARLELELQKIQYVLLKLEEEHEYTRLKVSDKRTRVLLRDYLYGRKDRGGGQKGKKKRVPFCGCVRSKSRTEP

Target ORF information:

RefSeq Version	XM_020546788.1
Organism	Zea mays
Definition	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020546788.1

ORF Insert Sequence:

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103643560 ( XM_020546785.1 ) cDNA ORF clone, Zea mays -> XP_020402374.1 Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X1, mRNA.

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CloneID	OZa55760
Clone ID Related Accession (Same CDS sequence)	XM_020546786.1 , XM_020546787.1 , XM_008666729.2 , XM_020546788.1 , XM_020546785.1
Accession Version	XM_020546785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	protein NETWORKED 1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020402374.1
CDS	387..8105
Translation	MATLVRHDSRQYSWWISHISPKNSKWLQENVSDMDSKVKAMIKLINEDADSFAKRAEMYYKKRPELMNLVEEFYRAYRALAERYDQSTGALRQAHRTISEAFPNQMPSMSDESPSAFSQEMEPHTPDTSTLTRVAFDYDDLQKDGVGVSPQSFTSKRNGTHPEETSALSSSVENAPRAGFDGKVWKGLTFEGPNVKGKDDISNEMANLQQEVARLLAESQDLKQQMFSESERANKAENEMQMLKATVLQLNAEKDTSLMQYNNSSERISTLESELSKAQSDLEKLTDEMAADVQKLINAETLNIAIQSEAEGLDQKMKMQQQALEQKLKELESFRSSFQEEHEKRMQAESALLSQRKELAQSHEEVQRLAIEIKTANEKLNEFKQTKEVLEYTVCELKKDVERLTEQNQSSEVLVQKLGDQINTLKDSNNELQSEIQSLKSIISQLNTEKNAAVLQHQQFVELVSVLESQLSKLQSELDETEQKVQMLTHDLEKKKEEADNVHLKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDGSTKKLSELEKNKLDLESILKELNNTILGLNSEKDATLLQQQQSLQKVSDLELELSKMQLEMEVLEDTVCELKKDVERHTEQNQSSEVLIQKLGEEINTIKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSIELVSVLESQLSKLHSELDEIEQKVQLLTHDLEKKKEEADNVHIKLQDECHRRMQIEATLLMTEGLHSQLQEEMQMLTQDFDRSTKKLSELENNKLDLESILKELNKTILGLNSEKDAALLQQQQYLQKVSDLELELSKMQLEMEVLEDTVCDLKKDVERHTEQNQSSEVLIQKLGEEINTLKDSKNELQSEIQSLKSIISQLNTEKNAAVLQHQQSVELVSVLESQLSKLQSELDETEQRVQLLTQDLEKKKEEADNVHLKLQDECHRRMQIEAALLMTEGLHSQLQEKMQMLTQDFDGSTKKLSELENNKLDLESTLKELNNTILGLNSEKDAALLQQQQSLQKVSNLELELSMMQLEMDKSEQKILLLEQEIARKNGSVDSLEISLKDECEKRLQAQKMYSQSQEDVSRLYIEIEKQNGKLNELENLISELNNTILLINTEKDATLHENQQSSVRISDLESELMALKTELENVEGKVHAVEQELIYKKEEADNLQISLQDEIQKRVEGEESLLMMTNLHSGSQNEVRGLALELEKLNGNLRQVENSKVDLENIVTKHTEEIHILRERNLSTDLMIKDLHLELDALKDLNVKLQTEMGLHIGEKEALQREFTSQREEKKNLEGIHHTLVDEMSTLKTTATMNQKLIEELQITNSKLKEVCAKSEVEKALLSEKLQEVEKLSEECSLLENSLSDANAEMDALRDKIKALEALESSLKDIISCHVSEKAVLVSELEILGKRLSDASEKNSIMDSSLFDMKMDLEDLRTKLKDSEESCQALLANNSALSGEVDALREKIKALQDSESSLKDAISCYVSEKAVLASELEILGKSLSEVSEKNSILDISLCDMKTELDELRTKLKDSEESCQAHLTNNSALSAEKNNLFSQLESITLAMKALEGMHANLEQKHSSVSREKDFAYDQVRELQDQLRIKNEEFEVSAKSHQLQANSYEKQIFSLQEKNHYMEEVLQQEQQKNINASISTVILENCLVDEQDKKVALFTECQKYAVENHSANMLVSELMEEARYHGEERKKLLAHNEKLREGISNQMKFLNICKDLGPADLAEDEVLLQTVSDETINIMRLKDETEDVNRLMDTELSVLSVVLLQVGMELRDLHLQKCALEKEVESGEKETLSLQNQNQQMLEQNEQLRSGLQESSEGVEVLKTEVFVIQEKLSCLRESYQTSQDEISNLTKKNESLSKEYQSLSEKYNALEDENDTIFEECMMLKNLCLFFRGHNNEIASALASLTDEMALLSLAKGDLDLQVNELNRRSVMLESENYHLKDYIIYLVEILRTRLVLSEFDLDTNRSVFQELVVELENCMTQLVQKDDELMEAEEKVQLLQEKNRELCGVVGSLQVAMEGAKVVKGELEKKITILIEQCSSKDDKILLLHQDNEALQSEIEQCEREFVELMDDAITFSVNSAVYEEKAFELMMNGKATENRAISQKELLMKEISSRDANIEELQKRLAGIQDEHAELKAELNTHLALIASLVDHVSVLEEDVRSLSKPCSTEGKEETARVHHVQEGDDGLESHCLVNGTPKLQGLIARIEALQVVVLNAKDRQDRKSAESAAKLAAASAEIQELKTRGGSRMEAKEICSDNEKQDVEVSKGKQVQIMKDIELDQISTCPPYGAGAALYPHGTGANAELDDDMLQLWEAAERSCKNQTAKSSSSEHDIQAVEDLESECPSSELARGRDLGTNKLEVPKGAVEPHEAWSKNVLERLTSDAQRLLSIQASIEELKKKMEGSSKGTNSEYGSVSAQVHETEGLVLEQINSNNKLSRKAENYPALSDNTSTERGGYSSRRKISEQVQRGSENVARLELELQKIQYVLLKLEEEHEYTRLKVSDKRTRVLLRDYLYGRKDRGGGQKGKKKRVPFCGCVRSKSRTEP

Target ORF information:

RefSeq Version	XM_020546785.1
Organism	Zea mays
Definition	Zea mays protein NETWORKED 1D (LOC103643560), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020546785.1

ORF Insert Sequence:

ATGGCGACTT TGGTCCGTCA TGATTCAAGG CAGTACTCGT GGTGGATTAG CCATATCAGC 
CCCAAGAACT CGAAATGGCT CCAGGAAAAT GTCAGCGACA TGGACTCAAA GGTTAAAGCA 
ATGATCAAAC TTATCAATGA AGATGCTGAT TCTTTTGCAA AGAGAGCAGA GATGTACTAC 
AAGAAACGTC CAGAGCTAAT GAATTTGGTG GAGGAATTCT ATCGAGCGTA CCGTGCACTG 
GCAGAAAGAT ACGATCAGTC CACTGGGGCA CTTCGCCAGG CTCACAGAAC AATTTCTGAA 
GCATTTCCAA ATCAGATGCC ATCAATGTCA GACGAGTCAC CCTCTGCTTT CAGCCAAGAG 
ATGGAGCCTC ATACTCCAGA TACGTCTACT CTCACACGTG TGGCATTTGA TTACGATGAT 
CTTCAGAAGG ATGGAGTTGG TGTGTCACCA CAAAGTTTCA CCTCCAAGAG GAATGGTACA 
CACCCTGAGG AAACCAGTGC GCTGTCATCG AGTGTTGAAA ATGCTCCTCG TGCTGGTTTT 
GATGGAAAGG TATGGAAAGG ACTAACTTTT GAAGGCCCAA ATGTCAAAGG GAAAGATGAT 
ATTAGCAACG AGATGGCAAA TTTGCAACAA GAAGTTGCAA GACTATTAGC TGAGAGCCAG 
GATCTGAAAC AGCAGATGTT TTCAGAATCC GAGCGGGCAA ACAAAGCTGA AAATGAGATG 
CAAATGCTCA AGGCAACAGT ATTGCAATTA AATGCTGAAA AAGACACATC ACTTATGCAA 
TATAATAATT CCTCTGAGCG AATATCTACA TTGGAGTCTG AGCTATCGAA GGCTCAGTCT 
GACCTTGAGA AGTTAACTGA TGAAATGGCT GCAGATGTCC AGAAGCTAAT TAATGCTGAA 
ACACTCAATA TTGCTATACA GTCTGAGGCT GAGGGTTTGG ATCAGAAGAT GAAGATGCAG 
CAACAAGCAC TAGAACAAAA GCTTAAGGAA TTGGAGAGTT TCCGCTCAAG CTTCCAGGAG 
GAGCATGAAA AGCGCATGCA GGCTGAAAGT GCTCTACTTT CCCAGAGGAA GGAACTTGCT 
CAATCTCATG AAGAAGTGCA GAGGTTGGCT ATAGAAATAA AGACAGCGAA TGAAAAGTTG 
AATGAGTTCA AGCAGACCAA GGAGGTCCTT GAGTACACTG TTTGTGAGCT GAAGAAGGAT 
GTTGAGCGCC TTACAGAACA AAACCAGTCC TCTGAGGTGC TTGTACAGAA ACTTGGTGAC 
CAGATTAATA CACTCAAGGA TTCAAATAAT GAACTTCAAA GTGAAATACA AAGCCTTAAG 
AGCATCATTT CACAGCTAAA CACTGAGAAG AATGCAGCTG TGCTTCAACA CCAGCAGTTT 
GTGGAACTGG TTTCGGTACT TGAATCTCAG TTGTCGAAGC TACAGTCAGA GCTAGATGAG 
ACTGAGCAGA AAGTACAGAT GCTGACACAT GATCTTGAAA AGAAGAAAGA AGAAGCGGAC 
AATGTCCATC TCAAGCTGCA AGATGAATGC CATAGGCGTA TGCAAATTGA GGCAACTCTT 
CTCATGACAG AGGGTCTGCA TTCCCAGTTG CAAGAAGAAA TGCAAATGTT GACACAAGAT 
TTTGACGGGT CAACAAAGAA GCTCAGTGAG TTGGAAAAAA ATAAGTTAGA CCTGGAGAGC 
ATACTGAAAG AACTGAATAA CACCATTTTA GGTTTGAACT CGGAGAAGGA TGCAACACTC 
CTTCAGCAAC AGCAGTCTTT ACAGAAAGTA TCTGATTTGG AGTTAGAACT CTCAAAGATG 
CAGTTGGAAA TGGAGGTCCT TGAAGACACT GTTTGTGAGC TGAAGAAGGA TGTTGAGCGC 
CATACAGAAC AAAACCAGTC CTCTGAGGTG CTTATACAGA AACTTGGTGA GGAGATAAAT 
ACAATCAAGG ATTCGAAGAA TGAACTTCAA AGTGAAATAC AAAGCCTTAA GAGCATCATT 
TCACAGCTAA ACACTGAGAA GAATGCAGCT GTGCTTCAAC ACCAGCAGTC TATAGAGCTG 
GTTTCGGTAC TCGAGTCTCA GCTGTCGAAG CTACACTCAG AGCTAGATGA GATTGAGCAG 
AAAGTACAGT TGCTGACACA TGATCTTGAA AAGAAGAAAG AAGAAGCGGA CAATGTCCAT 
ATCAAGCTGC AAGATGAATG CCATAGGCGT ATGCAAATTG AAGCAACTCT TCTCATGACA 
GAGGGTCTGC ATTCCCAGTT GCAAGAAGAA ATGCAAATGT TGACACAAGA TTTTGACAGG 
TCAACAAAGA AGCTCAGTGA GTTGGAAAAT AATAAGTTAG ACCTGGAGAG CATACTGAAA 
GAACTGAATA AGACCATTTT AGGTTTGAAC TCGGAGAAGG ATGCAGCACT CCTTCAGCAA 
CAGCAGTATT TACAGAAAGT ATCTGATTTG GAGTTAGAAC TCTCAAAGAT GCAGTTGGAA 
ATGGAGGTCC TTGAGGACAC TGTTTGTGAC CTGAAGAAGG ATGTTGAGCG CCATACAGAA 
CAAAACCAGT CCTCTGAGGT GCTTATACAG AAACTTGGTG AGGAGATAAA TACACTCAAG 
GATTCGAAGA ATGAACTTCA AAGTGAAATA CAAAGCCTTA AAAGCATCAT TTCACAGCTA 
AACACTGAGA AGAATGCAGC TGTGCTTCAA CACCAGCAGT CTGTGGAGCT GGTTTCGGTA 
CTCGAATCTC AGCTGTCAAA GCTACAGTCA GAGCTAGATG AGACTGAGCA GAGAGTACAG 
TTGCTAACAC AAGATCTTGA AAAGAAGAAA GAAGAAGCAG ATAATGTCCA TCTCAAGCTG 
CAAGATGAAT GCCATAGGCG TATGCAAATT GAAGCAGCTC TTCTCATGAC AGAGGGTCTG 
CATTCCCAGT TGCAAGAAAA AATGCAAATG TTGACACAAG ATTTTGATGG ATCAACAAAG 
AAGCTCAGTG AGTTGGAAAA TAATAAGTTA GACCTGGAGA GCACACTGAA AGAACTGAAT 
AACACCATTT TAGGTTTGAA CTCGGAGAAG GATGCAGCAC TCCTTCAACA ACAGCAGTCT 
TTACAGAAAG TATCTAATTT GGAGTTAGAA CTCTCAATGA TGCAGTTGGA AATGGACAAG 
TCTGAACAAA AAATTCTCTT ACTGGAACAA GAAATTGCAC GGAAGAATGG AAGTGTCGAC 
AGCCTGGAGA TAAGTTTGAA AGATGAATGT GAGAAGAGGC TACAAGCCCA AAAAATGTAT 
TCCCAGTCAC AGGAAGACGT TAGTAGGTTG TACATAGAGA TAGAGAAGCA GAATGGCAAG 
TTGAATGAGT TGGAGAACTT GATATCCGAA CTTAACAACA CCATCCTACT TATAAACACT 
GAGAAAGATG CCACCCTCCA TGAGAACCAG CAGTCCTCAG TGAGAATATC AGATCTTGAA 
TCTGAACTCA TGGCCTTGAA AACCGAACTG GAAAATGTTG AAGGGAAAGT TCATGCGGTA 
GAGCAAGAAC TCATATATAA GAAAGAAGAA GCAGATAACC TCCAAATTAG CCTACAAGAT 
GAAATTCAAA AACGAGTTGA AGGTGAAGAA TCTCTTCTTA TGATGACAAA TCTTCACTCT 
GGGTCACAAA ATGAAGTGAG AGGGTTGGCA TTGGAACTTG AGAAGCTGAA TGGTAATTTA 
AGACAGGTGG AGAACAGTAA GGTTGATCTT GAGAACATTG TAACCAAGCA TACCGAGGAA 
ATTCATATCC TTCGTGAACG GAATCTTTCT ACTGACCTTA TGATAAAGGA CCTTCATCTT 
GAATTGGACG CACTGAAGGA TTTGAACGTG AAACTTCAGA CAGAAATGGG GTTACACATA 
GGTGAAAAAG AAGCACTTCA GAGAGAATTT ACTTCTCAAA GGGAAGAGAA GAAAAATCTA 
GAGGGGATAC ACCACACTCT GGTTGATGAG ATGAGTACCC TAAAAACCAC TGCAACAATG 
AACCAGAAGC TGATTGAGGA ACTGCAAATT ACAAACTCAA AACTGAAGGA GGTGTGTGCA 
AAGAGTGAGG TTGAGAAGGC ACTTCTCTCA GAAAAACTCC AAGAGGTGGA GAAGCTATCT 
GAAGAATGTT CACTCTTGGA GAACTCACTT TCAGATGCAA ATGCTGAAAT GGATGCATTG 
AGGGATAAAA TAAAGGCACT TGAGGCTTTA GAAAGCTCTC TCAAGGATAT AATTTCCTGT 
CATGTTTCTG AGAAGGCTGT TCTTGTATCA GAGCTAGAGA TCCTTGGTAA AAGATTATCT 
GATGCTTCAG AGAAGAATTC TATCATGGAT AGCTCATTGT TTGATATGAA AATGGACCTA 
GAAGACTTGA GAACAAAGTT GAAAGATTCT GAAGAATCCT GTCAGGCTCT CCTTGCAAAT 
AATTCAGCTC TTTCTGGTGA AGTGGATGCA TTAAGAGAGA AGATAAAAGC ACTACAGGAC 
TCAGAAAGCT CTCTGAAGGA TGCAATTTCC TGTTATGTTT CTGAAAAGGC TGTTCTTGCA 
TCAGAGCTAG AGATCCTTGG TAAAAGTTTA TCTGAGGTTT CAGAGAAGAA CTCTATTTTG 
GATATCTCAT TATGTGATAT GAAAACTGAG CTAGATGAAT TGAGAACAAA GCTGAAAGAT 
TCTGAAGAAT CCTGCCAGGC TCACCTAACA AATAATTCAG CTCTTTCTGC TGAAAAGAAC 
AATCTGTTCT CTCAGTTGGA GAGCATTACA TTGGCTATGA AAGCCCTAGA AGGTATGCAT 
GCCAATCTGG AACAGAAGCA CTCCTCTGTA TCAAGAGAGA AGGATTTTGC ATATGATCAA 
GTAAGGGAAC TGCAGGATCA ATTGAGAATT AAGAACGAGG AATTTGAAGT GTCAGCAAAG 
TCACATCAAC TTCAAGCAAA CAGCTATGAG AAACAAATTT TTTCTCTACA AGAGAAAAAC 
CATTATATGG AGGAGGTGCT TCAACAGGAA CAACAGAAAA ACATAAATGC TTCTATTAGT 
ACAGTTATTT TGGAGAACTG TTTGGTCGAT GAGCAAGACA AGAAGGTTGC TCTTTTCACT 
GAATGCCAAA AGTATGCTGT GGAAAATCAT TCAGCCAATA TGTTAGTTTC AGAGTTAATG 
GAGGAAGCTA GATATCATGG GGAGGAGAGA AAAAAATTGC TAGCGCACAA TGAAAAACTG 
AGGGAAGGGA TTTCAAATCA AATGAAGTTT CTGAATATCT GCAAAGATCT AGGACCTGCT 
GATTTAGCTG AGGACGAAGT TCTGTTGCAG ACTGTTTCAG ATGAAACTAT TAACATTATG 
AGACTTAAGG ATGAAACTGA AGATGTGAAT AGGCTCATGG ACACCGAGCT CTCAGTTCTC 
TCAGTAGTTT TGTTGCAAGT AGGGATGGAG CTTAGAGACT TGCATTTACA GAAGTGTGCC 
CTTGAGAAAG AAGTGGAGAG TGGAGAAAAA GAGACGCTTT CTTTGCAAAA TCAAAACCAG 
CAGATGTTGG AACAGAATGA ACAACTAAGG AGTGGATTAC AGGAAAGCAG TGAAGGGGTG 
GAGGTGCTGA AAACTGAAGT ATTTGTGATA CAAGAGAAAC TATCTTGCTT GAGAGAGTCC 
TACCAGACCT CACAAGATGA AATTTCCAAC CTGACTAAAA AAAATGAGTC CTTGTCTAAG 
GAATACCAGT CCTTGAGTGA GAAGTACAAT GCCTTGGAAG ATGAGAATGA TACTATTTTT 
GAAGAATGCA TGATGCTCAA GAACTTGTGC CTGTTCTTTA GGGGACATAA TAATGAGATT 
GCATCTGCAT TGGCTTCTCT TACCGATGAG ATGGCTTTGT TGAGTTTGGC TAAGGGCGAC 
CTCGATCTCC AAGTTAATGA GCTGAACAGA AGGTCAGTAA TGCTTGAATC AGAAAATTAT 
CACTTAAAGG ACTATATCAT CTACTTGGTA GAGATTCTCA GGACTAGATT AGTCCTTTCA 
GAGTTTGATT TGGACACCAA TCGAAGTGTC TTCCAGGAGT TAGTTGTGGA ACTTGAGAAT 
TGCATGACAC AGTTGGTGCA GAAGGATGAT GAGCTAATGG AAGCAGAAGA GAAGGTTCAA 
CTCTTGCAAG AAAAGAACCG GGAACTCTGT GGAGTTGTTG GGTCTCTTCA GGTTGCAATG 
GAAGGTGCTA AAGTGGTGAA AGGAGAACTG GAGAAGAAAA TCACAATACT GATTGAACAG 
TGTTCTAGCA AGGACGACAA AATTTTACTT CTTCACCAAG ACAATGAGGC ATTGCAATCA 
GAGATTGAGC AATGTGAGAG GGAATTTGTT GAGCTGATGG ATGATGCCAT TACTTTTTCA 
GTGAATTCTG CGGTCTATGA AGAAAAAGCA TTCGAGCTTA TGATGAATGG TAAAGCTACA 
GAGAATAGAG CAATATCCCA AAAGGAGTTG CTAATGAAGG AGATCTCTTC TCGAGATGCT 
AATATTGAGG AGTTGCAGAA AAGGCTGGCT GGTATTCAGG ATGAACATGC AGAACTAAAA 
GCTGAGTTGA ACACACATCT AGCTCTCATA GCATCATTGG TTGATCATGT CAGTGTGCTG 
GAAGAAGATG TTCGTTCTCT GTCAAAACCA TGTAGTACAG AAGGCAAAGA GGAAACTGCA 
AGGGTACATC ATGTGCAAGA GGGCGACGAT GGGCTGGAAT CTCATTGCTT AGTCAATGGA 
ACTCCAAAGT TACAAGGGTT GATTGCAAGA ATAGAAGCAC TTCAAGTGGT TGTATTAAAT 
GCTAAAGATC GTCAAGATCG TAAATCAGCC GAGTCTGCAG CTAAACTGGC GGCAGCAAGT 
GCAGAAATTC AAGAGCTTAA AACAAGAGGC GGCTCACGCA TGGAGGCAAA AGAAATATGT 
TCTGACAATG AGAAACAAGA TGTTGAGGTC TCCAAAGGGA AGCAAGTCCA GATCATGAAG 
GATATTGAGC TGGACCAAAT ATCTACTTGC CCTCCATATG GCGCTGGAGC TGCCCTCTAC 
CCCCATGGAA CTGGTGCAAA TGCTGAACTA GATGACGATA TGCTTCAGCT GTGGGAGGCT 
GCGGAGAGGA GCTGCAAGAA CCAAACGGCC AAGTCCTCGT CATCTGAGCA CGACATACAG 
GCGGTCGAGG ATCTGGAGAG CGAGTGCCCT TCGTCTGAGC TAGCGAGGGG AAGAGACCTT 
GGAACCAATA AGCTAGAAGT GCCAAAGGGA GCTGTAGAGC CTCACGAGGC GTGGAGCAAG 
AACGTGCTTG AGAGGCTCAC TTCTGATGCC CAGAGGCTGT TGAGCATCCA AGCGAGCATC 
GAGGAACTGA AGAAGAAAAT GGAGGGGTCC TCGAAGGGTA CGAATTCTGA GTACGGCAGT 
GTGAGCGCTC AGGTGCATGA GACGGAGGGT CTTGTCTTGG AACAGATCAA TTCCAACAAC 
AAGTTGAGCA GGAAGGCTGA GAACTACCCC GCATTGTCAG ATAATACGAG CACCGAGCGA 
GGAGGGTATT CCAGCAGGAG GAAGATCTCG GAGCAGGTGC AGAGAGGGTC GGAAAACGTG 
GCAAGGTTGG AACTGGAGCT CCAGAAGATC CAGTACGTCT TGCTGAAGCT TGAGGAGGAG 
CACGAGTACA CAAGGCTCAA AGTCTCTGAT AAGCGCACTC GTGTGCTTTT GAGGGACTAC 
CTGTATGGAA GGAAGGACCG TGGAGGCGGG CAGAAGGGAA AGAAGAAGCG GGTCCCGTTC 
TGTGGTTGTG TTCGTTCGAA ATCAAGAACA GAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC103643560 cDNA ORF clone, Zea mays

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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